Skocz do pozycji: 1.

Tytuł:

The expression of UVR3 and two putative photolyases, PHR2 and At4g25290, is regulated by light

Autorzy:

Sztatelman, O.
Labuz, J.
Banas, A.K.

Powiązania:

https://bibliotekanauki.pl/articles/80793.pdf

Data publikacji:

2013

Wydawca:

Polska Akademia Nauk. Czytelnia Czasopism PAN

Tematy:

conference
DNA strand
RNA polymerase
DNA polymerase
replication
transcription
light regulation
putative photolyase

Źródło:

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2013, 94, 3
0860-7796

Pojawia się w:

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

Dostawca treści:

Biblioteka Nauki

/5

Artykuł

Zmień widok

• na półce

Skocz do pozycji: 2.

Tytuł:

Epi-genes potentiate plant biodiversity

Autorzy:

Szopa, J.

Powiązania:

https://bibliotekanauki.pl/articles/951285.pdf

Data publikacji:

2015

Wydawca:

Polska Akademia Nauk. Czytelnia Czasopism PAN

Tematy:

biodiversity
oligonucleotide
DNA methylation
gene expression
protein
methylase
polymerase
RNA polymerase

Źródło:

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2015, 96, 1
0860-7796

Pojawia się w:

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

Dostawca treści:

Biblioteka Nauki

/5

Artykuł

Zmień widok

• na półce

Skocz do pozycji: 3.

Tytuł:

Adaptation of PCR technique for quantitative estimation of genetic material from different regions of chromosome 21 in cases of trisomy 21.

Autorzy:

Nowacka, Joanna
Helszer, Zofia
Walter, Zofia
Płucienniczak, Andrzej
Kałużewski, Bogdan

Powiązania:

https://bibliotekanauki.pl/articles/1041513.pdf

Data publikacji:

2004

Wydawca:

Polskie Towarzystwo Biochemiczne

Tematy:

quantitative polymerase chain reaction
trisomy 21

Opis:

Pre- and postnatal diagnosis of chromosomal aberrations is generally based on conventional cytogenetic analysis. In this paper, we have devised a quantitative polymerase chain reaction (Q-PCR) method to determine gene dose effects and applied it in cases of regular trisomy 21 as a model. The method is based on quantitative assessment of PCR products after using primers amplifying DNA fragments located in the pericentromeric, heterochromatic, euchromatic and telomeric regions of chromosome 21. A gene dose effect on the amount of PCR product in cases of trisomy 21 was confirmed. Moreover, a correlation between the amount of the PCR product of the examined sequences and their location in the chromosome was observed. The obtained results suggest that the Q-PCR technique can be applied in the diagnosis of aneuploidies.

Źródło:

Acta Biochimica Polonica; 2004, 51, 4; 995-1001
0001-527X

Pojawia się w:

Acta Biochimica Polonica

Dostawca treści:

Biblioteka Nauki

/5

Artykuł

Zmień widok

• na półce

Skocz do pozycji: 4.

Tytuł:

Quantitative analysis of the ternary complex of RNA polymerase, cyclic AMP receptor protein and DNA by fluorescence anisotropy measurements

Autorzy:

Bonarek, Piotr
Kędracka-Krok, Sylwia
Kępys, Barbara
Wasylewski, Zygmunt

Powiązania:

https://bibliotekanauki.pl/articles/1040712.pdf

Data publikacji:

2008

Wydawca:

Polskie Towarzystwo Biochemiczne

Tematy:

cAMP receptor protein
fluorescence anisotropy
RNA polymerase

Opis:

The in vitro formation of transcription complexes with Escherichia coli RNA polymerase was monitored using fluorescence anisotropy measurements of labeled fragments of DNA. The multicomponent system consisted of holo or core RNA polymerase (RNAP) and lac or gal promoter fragments of DNA (in different configurations), in the presence or absence of CRP activator protein (wt or mutants) with its ligand, cAMP. Values of the apparent binding constants characterizing the system were obtained, as a result of all processes taking place in the system. The interaction of the promoters with core RNAP in the absence of CRP protein was characterized by apparent binding constants of 0.67 and 1.9 × 106 M-1 for lac166 and gal178, respectively, and could be regarded as nonspecific. The presence of wt CRP enhanced the strength of the interaction of core RNAP with the promoter, and even in the case of gal promoter it made this interaction specific (apparent binding constant 2.93 × 107 M-1). Holo RNAP bound the promoters significantly more strongly than core RNAP did (apparent binding constants 1.46 and 40.14 × 106 M-1 for lac166 and gal178, respectively), and the presence of CRP also enhanced the strength of these interactions. The mutation in activator region 1 of CRP did not cause any significant disturbances in the holo RNAP-lac promoter interaction, but mutation in activator region 2 of the activator protein substantially weakened the RNAP-gal promoter interaction.

Źródło:

Acta Biochimica Polonica; 2008, 55, 3; 537-547
0001-527X

Pojawia się w:

Acta Biochimica Polonica

Dostawca treści:

Biblioteka Nauki

/5

Artykuł

Zmień widok

• na półce

Skocz do pozycji: 5.

Tytuł:

Improved method of isolation of total nucleic acids from hop plants and grapevine before the RT-PCR by addition of polyvinylpolypyrrolidone
Usprawniona metoda izolacji calkowitych kwasow nukleinowych z chmielu i winorosli przez RT-PCR przez dodanie poliwinylopolipirolidonu

Autorzy:

Cajza, M
Folkman, W.

Powiązania:

https://bibliotekanauki.pl/articles/65582.pdf

Data publikacji:

2003

Wydawca:

Polska Akademia Nauk. Czytelnia Czasopism PAN

Tematy:

isolation
total nucleic acid
nucleic acid
hop plant
grapevine
RT-PCR method zob.reverse transcription polymerase chain reaction
addition
polyvinylpolypyrrolidone
reverse transcription polymerase chain reaction
polymerase chain reaction

Źródło:

Journal of Plant Protection Research; 2003, 43, 4; 375-380
1427-4345

Pojawia się w:

Journal of Plant Protection Research

Dostawca treści:

Biblioteka Nauki

/5

Artykuł

Zmień widok

• na półce

Skocz do pozycji: 6.

Tytuł:

Cloning and expression of the two DNA fragments of the I-18 C region of Chironomus tentans. II. The effects of the applied technology

Autorzy:

Borowicz, B P

Powiązania:

https://bibliotekanauki.pl/articles/66157.pdf

Data publikacji:

1998

Wydawca:

Polska Akademia Nauk. Czytelnia Czasopism PAN

Tematy:

promoter system
cloning
I-18 C gene
T7 RNA polymerase
Chironomus tentans
polymerase chain reaction
DNA fragment

Opis:

The chromosomal I-18 C region of Chironomus tentans contains the I-18 C gene. Two different open reading frames (i.e. the ORF I and II) of two different transcripts (i.e. the 1.8 kb and 4.6 kb RNA) of the I-18 C gene of Chironomus tentans were isolated, at the DNA level, by the Polymerase Chain Reaction, then were cloned into the bluescript vector and finally were cloned into the pET-3a vector in order to translate them in T7 RNA polymerase / promoter system of E. coli. It was possible to obtain the ORF I overexpressed. In a case of the ORF II the low molecular weight polypeptide was detected, however it was not overexpressed, but still this polypeptide was strongly visible on the SDS-polyacrylamide gel.
Region chromosomalny I-18 C Chironomus tentans zawiera gen I-18 C. Dwie odrębne otwarte ramy odczytu (tj. ORF I i II) genu I-18 C Chironomus tentans zostały wyizolowane, na poziomie DNA, przy użyciu Reakcji Łańcuchowej Polimerazy (PCR), a następnie zostały wklonowane do wektora blueskrypt i ostatecznie zostały wklonowane do wektora pET-3a w celu ich translacji w systemie promotora T7 RNA polimerazy w E. coli. Uzyskano bardzo dużą ekspresję ORF I. W przypadku ORF II wykryto obecność polipeptydu o niskiej masie cząsteczkowej i chociaż nie uzyskano jego bardzo dużej ekspresji, tym niemniej polipeptyd ten był silnie widoczny w żelu poliakryloamidowym z SDS.

Źródło:

Journal of Plant Protection Research; 1998, 38, 1
1427-4345

Pojawia się w:

Journal of Plant Protection Research

Dostawca treści:

Biblioteka Nauki

/5

Artykuł

Zmień widok

• na półce

Skocz do pozycji: 7.

Tytuł:

Properties of Escherichia coli RNA polymerase from a strain devoid of the stringent response alarmone ppGpp

Autorzy:

Szalewska-Pałasz, Agnieszka

Powiązania:

https://bibliotekanauki.pl/articles/1040748.pdf

Data publikacji:

2008

Wydawca:

Polskie Towarzystwo Biochemiczne

Tematy:

RNA polymerase
ppGpp
stringent response
transcription

Opis:

The stringent response alarmone guanosine tetraphosphate (ppGpp) affects transcription from many promoters. ppGpp binds directly to the transcription enzyme of Escherichia coli, RNA polymerase. Analysis of the crystal structure of RNA polymerase with ppGpp suggested that binding of this nucleotide may result in some conformational or post-translational alterations to the enzyme. These changes might affect in vitro performance of the enzyme. Here, a comparison of the in vitro properties of RNA polymerases isolated from wild type and ppGpp-deficient bacteria shows that both enzymes do not differ in i) transcription activity of various promoters (e.g. σ70-rrnB P1, λpL, T7A1), ii) response to ppGpp, iii) promoter-RNA polymerase open complex stability. Thus, it may be concluded that ppGpp present in the bacterial cell prior to purification of the RNA polymerase does not result in the alterations to the enzyme that could be permanent and affect its in vitro transcription capacity.

Źródło:

Acta Biochimica Polonica; 2008, 55, 2; 317-323
0001-527X

Pojawia się w:

Acta Biochimica Polonica

Dostawca treści:

Biblioteka Nauki

/5

Artykuł

Zmień widok

• na półce

Skocz do pozycji: 8.

Tytuł:

Effect of amyloid beta peptide on poly(ADP-ribose) polymerase activity in adult and aged rat hippocampus.

Autorzy:

Strosznajder, Joanna
Jęśko, Henryk
Strosznajder, Robert

Powiązania:

https://bibliotekanauki.pl/articles/1044342.pdf

Data publikacji:

2000

Wydawca:

Polskie Towarzystwo Biochemiczne

Tematy:

aging
amyloid
poly(ADP-ribose) polymerase
hippocampus
neurotoxicity

Opis:

It is suggested that the fibrillar amyloid beta peptide (Aβ) in brain plays a direct role in neurodegeneration in Alzheimer's disease, probably through activation of reactive oxygen species formation. Free radicals and numerous neurotoxins elicit DNA damage that subsequently activates poly(ADP-ribose) polymerase (PARP, EC 2.4.2.30). In this study the effect of neurotoxic fragment (25-35) of full length Aβ peptide on PARP activity in adult and aged rat hippocampus was investigated. In adult (4 month old) rat hippocampus the Aβ 25-35 peptide significantly enhanced PARP activity by about 80% but had no effect on PARP activity in cerebral cortex and in hippocampus from aged (24-27 month old) rats. The effect of Aβ peptide was reduced by half by the nitric oxide synthase inhibitor N-nitro-L-arginine. Stimulation of glutamate receptor(s) itself enhanced PARP activity by about 80% in adult hippocampus. However, Aβ 25-35 did not exert any additional stimulatory effect. These results indicate that Aβ, through NO and probably other free radicals, induces activation of DNA bound PARP activity exclusively in adult but not in aged hippocampus.

Źródło:

Acta Biochimica Polonica; 2000, 47, 3; 847-854
0001-527X

Pojawia się w:

Acta Biochimica Polonica

Dostawca treści:

Biblioteka Nauki

/5

Artykuł

Zmień widok

• na półce

Skocz do pozycji: 9.

Tytuł:

Polymorphism in Syringa rDNA regions assessed by PCR technique

Autorzy:

Smolik, M.
Andrys, D.
Franas, A.
Krupa-Malkiewicz, M.
Malinowska, K.

Powiązania:

https://bibliotekanauki.pl/articles/41641.pdf

Data publikacji:

2010

Wydawca:

Polska Akademia Nauk. Instytut Dendrologii PAN

Tematy:

polymorphism
lilac
Syringa
rDNA region
polymerase chain reaction

Opis:

The Syringa genus is characterizedby a multiplicity of forms. Its chief asset is the ornamental value of thousands of accessions, species or hybrids. From a phylogenetic point of view the genus is difficult in an explicit classification due to its frequently complex genome. The aim of this study was to determine the possibility for the identification of genotypic diversity and genetic relationships in the nrDNA sequence of some selected Syringa accessions – part of a collection of the Dendrological Garden in Przelewice (Poland). For this purpose, the PCR technique together with a combination of various ‘universal’ primers designed for the nrDNA sequence analysis were employed. Fourteen Syringa accessions: Syringa × chinensis Willd., S. × prestoniae Mc Kelv., S. × prestoniae ‘Telimena’, S. × prestoniae ‘Jaga’, S. × prestoniae ‘Basia’, S. meyeri ‘Palibin’, S. vulgaris ‘Miss Ellen Willmott’, S. vulgaris, S. vulgaris ‘Jules Simon’, S. vulgaris ‘Katherine Havemeyer’, S. vulgaris ‘Krasawica Moskvy’, S. vulgaris ‘Mirabeau’, S. vulgaris ‘Madame Lemoine’ and S. vulgaris ‘Niebo Moskvy’ made up the research material. In the conducted amplifications, genetic profiles were obtained for 14 combinations among the 25 combinations of different pairs of primers used. The nrDNA templates coding the small subunit (SSU), 5.8S subunit andITS1, ITS2 andIGS sequences were amplified. In PCR reactions a total of 33 PCR products were generated, of which 21 (64%) products were polymorphic, 6 (18%) monomorphic and6 (18%) were genotype-specific. For the lilac accessions examined246 amplicons were generated from ~230 to ~1100 bp in length. The analysis of both the dendrogram and the genetic similarity matrix revealedlow diversity between the examinedaccessions. For most they rangedfrom 70 to 80%, andthe greatest diversity (87%) was foundbetween the S. × prestoniae: ‘Basia’ and‘Telimena’ accessions, while the lowest (57%) was observed between S. vulgaris ‘Katherine Havermeyer’ and S. × chinensis.

Źródło:

Dendrobiology; 2010, 64
1641-1307

Pojawia się w:

Dendrobiology

Dostawca treści:

Biblioteka Nauki

/5

Artykuł

Zmień widok

• na półce

Skocz do pozycji: 10.

Tytuł:

Evaluation of the activity of thermostable DNA polymerases in the presence of heme, as a key inhibitor in the real time PCR method in diagnostics of sepsis

Autorzy:

Gosiewski, Tomasz
Brzychczy-Włoch, Monika
Pietrzyk, Agata
Sroka, Agnieszka
Bulanda, Małgorzata

Powiązania:

https://bibliotekanauki.pl/articles/1039451.pdf

Data publikacji:

2013

Wydawca:

Polskie Towarzystwo Biochemiczne

Tematy:

polymerase inhibitor
heme
real time PCR
sepsis

Opis:

The study aim was evaluation of the usefulness of several thermostable DNA polymerases in real time PCR conducted in the presence of the heme. Our study had the advantage of testing several different polymerases, one of which proved to be the least sensitive to heme activity. We also found that there is no need of supplementing the reaction mixture with protective substances like BSA. Selection of the appropriate polymerase can increase the efficiency of the PCR reaction which is very important for diagnosis of sepsis and for other analyses performed on DNA template isolated from the blood.

Źródło:

Acta Biochimica Polonica; 2013, 60, 4; 603-606
0001-527X

Pojawia się w:

Acta Biochimica Polonica

Dostawca treści:

Biblioteka Nauki

/5

Artykuł

Zmień widok

• na półce

Skocz do pozycji: 11.

Tytuł:

Pathways of tRNA turnover in eukaryotic cells

Autorzy:

Turowski, T. W.

Powiązania:

https://bibliotekanauki.pl/articles/115770.pdf

Data publikacji:

2011

Wydawca:

Fundacja na Rzecz Młodych Naukowców

Tematy:

tRNA turnover
rapid tRNA decay
polymerase III

Opis:

Cell ability to control amount of a transfer RNA is one of the ways to regulate rate of protein synthesis. Because 80–90% dry mass of cells are proteins, the level of translation is determinant to the cell growth. Growth of cells is a key question in tumors therapy and biotechnology. tRNA turnover consist of a three pathways described in a last few years: exosome and TRAMP complex dependent pathway in nucleus, directed to hypomodified or affected tRNA; rapid tRNA decay pathway involving two 5’–3’ exonucleases Rat1 and Xrn1, proposed to occur in nucleus and cytoplasm; stress-activated endonucleolytic cleavage to tRNA halves pathway, founded in cytoplasm with a clear role to direct regulation of translation by tRNA half-molecules inhibition.

Źródło:

Challenges of Modern Technology; 2011, 2, 2; 63-65
2082-2863
2353-4419

Pojawia się w:

Challenges of Modern Technology

Dostawca treści:

Biblioteka Nauki

/5

Artykuł

Zmień widok

• na półce

Skocz do pozycji: 12.

Tytuł:

Application of polymerase chain reaction-restriction fragment length polymorphism (RFLP-PCR) in the analysis of single nucleotide polymorphisms (SNPs)

Autorzy:

Tarach, Piotr

Powiązania:

https://bibliotekanauki.pl/articles/1830648.pdf

Data publikacji:

2021-09-29

Wydawca:

Uniwersytet Łódzki. Wydawnictwo Uniwersytetu Łódzkiego

Tematy:

nucleotide polymorphisms
DNA analysis
polymerase chain reaction

Opis:

Polymerase chain reaction-restriction fragment length polymorphism (RFLP-PCR) is a technique used to identify single nucleotide polymorphisms (SNPs) based on the recognition of restriction sites by restriction enzymes. RFLP-PCR is an easy-to-perform and inexpensive tool for initial analysis of SNPs potentially associated with some monogenic diseases, as well as in genotyping, genetic mapping, lineage screening, forensics and ancient DNA analysis. The RFLP-PCR method employs four steps: (1) isolation of genetic material and PCR; (2) restriction digestion of amplicons; (3) electrophoresis of digested fragments; and (4) visualisation. Despite its obsolescence and the presence of high-throughput DNA analysis techniques, it is still applied in the analysis of SNPs associated with disease entities and in the analysis of genetic variation of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). RFLP-PCR is a low-cost and low-throughput research method allowing for the analysis of SNPs in the absence of specialised equipment, and it is useful when there is a limited budget.

Źródło:

Acta Universitatis Lodziensis. Folia Biologica et Oecologica; 2021, 17; 48-53
1730-2366
2083-8484

Pojawia się w:

Acta Universitatis Lodziensis. Folia Biologica et Oecologica

Dostawca treści:

Biblioteka Nauki

/5

Artykuł

Zmień widok

• na półce

Skocz do pozycji: 13.

Tytuł:

Cloning of the fragments reflecting the open reading frame I and II of the I-18 C gene of Chironomus tentans. IX. The effects of the cloning experiments

Autorzy:

Borowicz, B P

Powiązania:

https://bibliotekanauki.pl/articles/65584.pdf

Data publikacji:

1999

Wydawca:

Polska Akademia Nauk. Czytelnia Czasopism PAN

Tematy:

pET-3a vector
promoter system
cloning
I-18 C gene
T7 RNA polymerase
Chironomus tentans
polymerase chain reaction
cloning experiment

Opis:

The goal of these experiments was to clone the open reading frame (ORF) I and II reflecting DNA fragments of the I-18 C gene of Chironomus tentans into the pET-3a vector to translate them in the T7 RNA polymerase / promoter system in BL21(DE3) cells of E. coli. This goal has been achieved and the results of the cloning experiments are presented and discussed.
Celem wykonanych eksperymentów było wklonowanie fragmentów DNA odzwierciedlających otwartą ramę odczytu I i II genu I-18 C Chironomus tentans, do wektora pET-3a w celu ich translacji w systemie promotora T7 RNA polimerazy w komórkach E. coli szczepu BL21(DE3). Cel ten został zrealizowany, a wyniki eksperymentów klonowania przedstawiono i omówiono.

Źródło:

Journal of Plant Protection Research; 1999, 39, 1
1427-4345

Pojawia się w:

Journal of Plant Protection Research

Dostawca treści:

Biblioteka Nauki

/5

Artykuł

Zmień widok

• na półce

Skocz do pozycji: 14.

Tytuł:

Advanced methods of bacteriological identification in a clinical microbiology laboratory

Autorzy:

Zukowska, M.E.

Powiązania:

https://bibliotekanauki.pl/articles/2098275.pdf

Data publikacji:

2020

Wydawca:

Instytut Medycyny Wsi

Tematy:

clinical microbiology
molecular method
modern method
multiplex polymerase chain reaction
real-time polymerase chain reaction
next generation sequencing
MALDI-TOF mass spectrometry

Opis:

Introduction and objective. Conventional, culture-based methods of bacterial identification and drug-susceptibility testing are considered the gold standard in medical microbiology. In recent years, classical microbiological methods have been supplemented with modern analytical and molecular methods. The aim of the review was to discusses the methods which have been permanently adapted to bacteriological microbiological diagnostics. Abbreviated description of the state of knowledge. Currently, PCR, as well as other nucleic acid amplification tests and sequencing techniques, are part of the standard repertoire of microbiological diagnostics. With regard to the quality and speed of pathogen identification, the introduction of mass spectrometry techniques into routine microbiological diagnostics work-up has been revolutionary. Within a short time in many laboratories, Matrix-Assisted Laser Desorption/Ionisation – Time of Flight Mass Spectrometry (MALDI TOF MS) systems have almost completely replaced conventional biochemical pathogen identification. Conclusions. Microbiological diagnostics is an indispensable element of a targeted therapy. The techniques used in the laboratory depend primarily on the laboratory’s apparatus, the costs of the analysis, as well as the sensitivity and specificity of a method. However, regardless of the culture-based methods universality, advanced techniques have permanently established themselves in diagnostics. Confident information about the detected organism and treatment possibilities in a combination with the clinical context are conducive to successful therapy. Although modern methods still require validation and close collaboration between clinicians, microbiologists and bioinformaticians, these methods, once deemed to be the future, have already arrived.

Źródło:

Journal of Pre-Clinical and Clinical Research; 2021, 15, 2; 68-72
1898-2395

Pojawia się w:

Journal of Pre-Clinical and Clinical Research

Dostawca treści:

Biblioteka Nauki

/5

Artykuł

Zmień widok

• na półce

Skocz do pozycji: 15.

Tytuł:

Molecular diagnostics of Sarcocystis spp. infections

Autorzy:

Stojecki, K.
Karamon, J.
Sroka, J.
Cencek, T.

Powiązania:

https://bibliotekanauki.pl/articles/2088002.pdf

Data publikacji:

2012

Wydawca:

Polska Akademia Nauk. Czytelnia Czasopism PAN

Tematy:

molecular diagnostics
Sarcocystis
infection
sarcocystosis
polymerase chain reaction

Opis:

Protozoa of the genus Sarcocystis (phylum Apicomplexa, family Sarcocystidae) is one of the most common parasites affecting animals. Interspecies diagnostic of Sarcocystis genus was based on electron microscopy for many years. Because of absence of visible differences between species with reachable magnifications, light microscopy is useless. In many cases serological diagnostic method have lack of sensitivity. A variety of molecular methods have been developed and used to detect and identify Sarcocystis spp. and to assess the genetic diversity among this protozoan from different population/hosts. Nowadays, molecular diagnostic is the common, time/cost effective method used all over the world to interspecies differentiation.

Źródło:

Polish Journal of Veterinary Sciences; 2012, 15, 3
1505-1773

Pojawia się w:

Polish Journal of Veterinary Sciences

Dostawca treści:

Biblioteka Nauki

/5

Artykuł

Zmień widok

• na półce

Skocz do pozycji: 16.

Tytuł:

Differential stability of mitochondrial mRNA in HeLa cells

Autorzy:

Piechota, Janusz
Tomecki, Rafał
Gewartowski, Kamil
Szczęsny, Roman
Dmochowska, Aleksandra
Kudła, Marek
Dybczyńska, Lien
Stepien, Piotr
Bartnik, Ewa

Powiązania:

https://bibliotekanauki.pl/articles/1041282.pdf

Data publikacji:

2006

Wydawca:

Polskie Towarzystwo Biochemiczne

Tematy:

polyadenylation
HeLa
mitochondria
poly(A) polymerase
mRNA turnover

Opis:

The physiological significance and metabolism of oligoadenylated and polyadenylated human mitochondrial mRNAs are not known to date. After study of eight mitochondrial transcripts (ND1, ND2, ND3, ND5, CO1, CO2, ATP6/8 and Cyt. b) we found a direct correlation between the half-lives of mitochondrial mRNAs and their steady-state levels. Investigation of the mt-mRNA decay after thiamphenicol treatment indicated that three transcripts (ND2, ND3 and Cyt. b) are significantly stabilized after inhibition of mitochondrial translation. Careful analysis one of them, ND3, showed that inaccurate processing of the H-strand RNA precursor may occasionally occur between the ND3 and tRNAArg locus leading to synthesis of ND3 mRNAs lacking the STOP codon. However, analysis of the oligo(A) fraction observed in case of the ND3 indicates that partially polyadenylated mRNAs are linked rather to the transcription process than to the translation-dependent deadenylation. Analysis of ND3 mRNA turnover in cells with siRNA-mediated knock-down of the mitochondrial poly(A) polymerase shows that strongly decreased polyadenylation does not markedly affect the decay of this transcript. We present a model where oligoadenylated mitochondrial transcripts are precursors of molecules containing full length poly(A) tails.

Źródło:

Acta Biochimica Polonica; 2006, 53, 1; 157-168
0001-527X

Pojawia się w:

Acta Biochimica Polonica

Dostawca treści:

Biblioteka Nauki

/5

Artykuł

Zmień widok

• na półce

Skocz do pozycji: 17.

Tytuł:

Mutations in DNA polymerase gamma cause error prone DNA synthesis in human mitochondrial disorders

Autorzy:

Copeland, William
Ponamarev, Mikhail
Nguyen, Dinh
Kunkel, Thomas
Longley, Matthew

Powiązania:

https://bibliotekanauki.pl/articles/1043659.pdf

Data publikacji:

2003

Wydawca:

Polskie Towarzystwo Biochemiczne

Tematy:

aging
DNA replication
mitochondria
DNA repair
DNA polymerase

Opis:

This paper summarizes recent advances in understanding the links between the cell's ability to maintain integrity of its mitochondrial genome and mitochondrial genetic diseases. Human mitochondrial DNA is replicated by the two-subunit DNA polymerase γ (pol γ). We investigated the fidelity of DNA replication by pol γ with and without exonucleolytic proofreading and its p55 accessory subunit. Pol γ has high base substitution fidelity due to efficient base selection and exonucleolytic proofreading, but low frameshift fidelity when copying homopolymeric sequences longer than four nucleotides. Progressive external ophthalmoplegia (PEO) is a rare disease characterized by the accumulation of large deletions in mitochondrial DNA. Recently, several mutations in the polymerase and exonuclease domains of the human pol γ have been shown to be associated with PEO. We are analyzing the effect of these mutations on the human pol γ enzyme. In particular, three autosomal dominant mutations alter amino acids located within polymerase motif B of pol γ. These residues are highly conserved among family A DNA polymerases, which include T7 DNA polymerase and E. coli pol I. These PEO mutations have been generated in pol γ to analyze their effects on overall polymerase function as well as the effects on the fidelity of DNA synthesis. One mutation in particular, Y955C, was found in several families throughout Europe, including one Belgian family and five unrelated Italian families. The Y955C mutant pol γ retains a wild-type catalytic rate but suffers a 45-fold decrease in apparent binding affinity for the incoming dNTP. The Y955C derivative is also much less accurate than is wild-type pol γ, with error rates for certain mismatches elevated by 10- to 100-fold. The error prone DNA synthesis observed for the Y955C pol γ is consistent with the accumulation of mtDNA mutations in patients with PEO. The effects of other pol γ mutations associated with PEO are discussed.

Źródło:

Acta Biochimica Polonica; 2003, 50, 1; 155-167
0001-527X

Pojawia się w:

Acta Biochimica Polonica

Dostawca treści:

Biblioteka Nauki

/5

Artykuł

Zmień widok

• na półce

Skocz do pozycji: 18.

Tytuł:

Effects of distortions by A-tracts of promoter B-DNA spacer region on the kinetics of open complex formation by Escherichia coli RNA polymerase.

Autorzy:

Kolasa, Iwona
Łoziński, Tomasz
Wierzchowski, Kazimierz

Powiązania:

https://bibliotekanauki.pl/articles/1043363.pdf

Data publikacji:

2003

Wydawca:

Polskie Towarzystwo Biochemiczne

Tematy:

RNA polymerase-promoter interaction
A-tract
DNA bending
kinetics of open complex formation
promoter spacer region
Escherichia coli RNA polymerase

Opis:

A-tracts in DNA due to their structural morphology distinctly different from the canonical B-DNA form play an important role in specific recognition of bacterial upstream promoter elements by the carboxyl terminal domain of RNA polymerase α subunit and, in turn, in the process of transcription initiation. They are only rarely found in the spacer promoter regions separating the -35 and -10 recognition hexamers. At present, the nature of the protein-DNA contacts formed between RNA polymerase and promoter DNA in transcription initiation can only be inferred from low resolution structural data and mutational and crosslinking experiments. To probe these contacts further, we constructed derivatives of a model Pa promoter bearing in the spacer region one or two An (n = 5 or 6) tracts, in phase with the DNA helical repeat, and studied the effects of thereby induced perturbation of promoter DNA structure on the kinetics of open complex (RPo) formation in vitro by Escherichia coli RNA polymerase. We found that the overall second-order rate constant ka of RPo formation, relative to that at the control promoter, was strongly reduced by one to two orders of magnitude only when the A-tracts were located in the nontemplate strand. A particularly strong 30-fold down effect on ka was exerted by nontemplate A-tracts in the -10 extended promoter region, where an involvement of nontemplate TG (-14, -15) sequence in a specific interaction with region 3 of σ-subunit is postulated. A-tracts in the latter location caused also 3-fold slower isomerization of the first closed transcription complex into the intermediate one that precedes formation of RPo, and led to two-fold faster dissociation of the latter. All these findings are discussed in relation to recent structural and kinetic models of RPo formation.

Źródło:

Acta Biochimica Polonica; 2003, 50, 4; 909-920
0001-527X

Pojawia się w:

Acta Biochimica Polonica

Dostawca treści:

Biblioteka Nauki

/5

Artykuł

Zmień widok

• na półce

Skocz do pozycji: 19.

Tytuł:

Regulation of RNA polymerase III transcription by Maf1 protein

Autorzy:

Cieśla, Małgorzata
Boguta, Magdalena

Powiązania:

https://bibliotekanauki.pl/articles/1040733.pdf

Data publikacji:

2008

Wydawca:

Polskie Towarzystwo Biochemiczne

Tematy:

transcription regulation
RNA polymerase III
tRNA synthesis
Maf1 repressor

Opis:

Maf1 was the first protein discovered to regulate polymerase III RNA in yeast and because it is evolutionarily conserved, a Maf1 ortholog also serves to restrain transcription in mouse and human cells. Understanding the mechanism of the regulation has been made possible by recent studies showing that Maf1 is a nuclear/cytoplasmic protein whose subcellular distribution and hence negative regulation of Pol III transcription is mediated by the nutrient-sensing signaling pathways, TOR and RAS. Under stress conditions and during growth in a nonfermentable carbon source Maf1 is dephosphorylated and imported to the nucleus. In its non-phosphorylated form, Maf1 interacts with the polymerase III transcription machinery. Phosphorylation serves to locate Maf1 to the cytoplasm under favorable growth conditions, thereby preventing it from non-negatively regulating polymerase III when high levels of tRNA transcription are required. Relocation of Maf1 to the cytoplasm is dependent on Msn5, a carrier responsible for export of several other phosphoproteins out of the nucleus. The absence of Maf1-mediated control of tRNA synthesis impairs yeast viability in nonfermentable carbon sources. Moreover, in cells grown in a nonfermentable carbon source, Maf1 regulates the levels of different tRNAs to various extents. This differential regulation may contribute to the physiological role of Maf1.

Źródło:

Acta Biochimica Polonica; 2008, 55, 2; 215-225
0001-527X

Pojawia się w:

Acta Biochimica Polonica

Dostawca treści:

Biblioteka Nauki

/5

Artykuł

Zmień widok

• na półce

Skocz do pozycji: 20.

Tytuł:

Detection of bovine leucocyte adhesion deficiency [BLAD] carriers using a new PCR test

Autorzy:

Kaminski, S
Czarnik, U

Powiązania:

https://bibliotekanauki.pl/articles/2046599.pdf

Data publikacji:

1997

Wydawca:

Polska Akademia Nauk. Czytelnia Czasopism PAN

Tematy:

cattle
bovine leucocyte adhesion deficiency
polymerase chain reaction
bacterial infection

Opis:

In this report we demonstrate a simple, effective and reliable diagnostic test of BLAD carrier detection based on specific PCR amplification of a 367 bp CD18 gene fragment and RFLP analysis using Taq I restriction enzyme. In a non-random population of 220 animals we found 48 BLAD carriers. Within the amplified PCR fragment an unknown intron sequence of 159 bp was identified.

Źródło:

Journal of Applied Genetics; 1997, 38, 1; 51-55
1234-1983

Pojawia się w:

Journal of Applied Genetics

Dostawca treści:

Biblioteka Nauki

/5

Artykuł

Zmień widok

• na półce

Skocz do pozycji: 21.

Tytuł:

Vegetable oil plant wastewater treatment by bacterial isolates : a study in the city of Hila, Iraq

Autorzy:

Salim, Hanan Kareem
Al-Ahmed, Suad Ghali Kadhim

Powiązania:

https://bibliotekanauki.pl/articles/2048496.pdf

Data publikacji:

2021

Wydawca:

Instytut Technologiczno-Przyrodniczy

Tematy:

biotreatment
polymerase chain reaction
PCR
sewage
vegetable oil plant
wastewater

Opis:

The present study was to reflect the use of some bacteria in the treatment and removal of pollutants in three selected wastewater sites, including a vegetable oil plant (viz. Al-Etihad Food Industries), the main wastewater treatment station in the city of Hila, and Al-Hila River water from October 2019 to January 2020. The bacterial isolates identified in these three sites were Klebsiella pneumoniae, Escherichia coli, Enterobacteria cloacae, Pseudomonas aeruginosa, Thalasobacillus devorans, Acinetobacter baumannii, and Bacillus subtilis. The molecular study of the bacterial isolates involved the detection of bacterial genera using the polymerase chain reaction (PCR). The results showed that water had a variable nature, depending on the substances in it. It recorded varying chemical and physical property values, ranging between 6.36 and 7.82 for pH and from 2500 to 7100 mg∙dm-3 for total alkalinity. Additional values were 713–2051 μS∙cm-1 for electrical conductivity (EC), 5.90–9.80 mg∙dm-3 for chemical oxygen demand (COD), and 480–960 mg∙dm-3 for total hardness. The given values were also 0.20–0.65 μg∙dm-3, 0.03-0.23 μg∙dm-3, and 0–107 mg∙dm-3 for nitrite (NO2), phosphate (PO4) oils, respectively.

Źródło:

Journal of Water and Land Development; 2021, 51; 163-167
1429-7426
2083-4535

Pojawia się w:

Journal of Water and Land Development

Dostawca treści:

Biblioteka Nauki

/5

Artykuł

Zmień widok

• na półce

Skocz do pozycji: 22.

Tytuł:

Hantavirus RNA not detected in Ixodes ricinus ticks

Autorzy:

Wojcik-Fatla, A.
Zajac, V.
Knap, J.P.
Dutkiewicz, J.

Powiązania:

https://bibliotekanauki.pl/articles/49890.pdf

Data publikacji:

2011

Wydawca:

Instytut Medycyny Wsi

Tematy:

hantavirus
RNA
Ixodes ricinus
tick
epidemiology
polymerase chain reaction
Polska

Źródło:

Annals of Agricultural and Environmental Medicine; 2011, 18, 2
1232-1966

Pojawia się w:

Annals of Agricultural and Environmental Medicine

Dostawca treści:

Biblioteka Nauki

/5

Artykuł

Zmień widok

• na półce

Skocz do pozycji: 23.

Tytuł:

Isolation, Identification and Preservation of Pectinolytic Bacteria Pathogenic to Potato

Autorzy:

Lebecka, Renata

Powiązania:

https://bibliotekanauki.pl/articles/2199695.pdf

Data publikacji:

2017-06-20

Wydawca:

Instytut Hodowli i Aklimatyzacji Roślin

Tematy:

Blackleg
Dickeya
Pectobacterium
Polymerase Chain Reaction
selective medium
soft rot

Opis:

Blackleg of potato plants and soft rot of tubers are caused by several species of pectinolytic bacte-ria from genera Pectobacterium and Dickeya. The text describes simple methods of isolating bacteria from symptomatic and symptomless organs of potato plants, their identification using Polymerase Chain Reaction (PCR) and preservation.

Źródło:

Plant Breeding and Seed Science; 2017, 75; 87-96
1429-3862
2083-599X

Pojawia się w:

Plant Breeding and Seed Science

Dostawca treści:

Biblioteka Nauki

/5

Artykuł

Zmień widok

• na półce

Skocz do pozycji: 24.

Tytuł:

The influence of LDHA gene polymorphism on relative level of its expression in racing pigeons
Polimorfizm w genie LDHA i jego wpływ na względny poziom ekspresji u gołębi pocztowych

Autorzy:

Jędrzejczak-Silicka, M.
Yu, Y.-H.
Cheng, Y.-H.
Dybus, A.

Powiązania:

https://bibliotekanauki.pl/articles/2606361.pdf

Data publikacji:

2018

Wydawca:

Zachodniopomorski Uniwersytet Technologiczny w Szczecinie. Wydawnictwo Uczelniane ZUT w Szczecinie

Tematy:

gene polymorphism
gene expression
pigeon
real-time polymerase chain reaction

Źródło:

Acta Scientiarum Polonorum. Zootechnica; 2018, 17, 3; 9-15
1644-0714

Pojawia się w:

Acta Scientiarum Polonorum. Zootechnica

Dostawca treści:

Biblioteka Nauki

/5

Artykuł

Zmień widok

• na półce

Skocz do pozycji: 25.

Tytuł:

Cloning of the DNA fragments reflecting the open reading frame I and II of the I-18 C gene of Chironomus tentans. V. Identification of the bacterial colonies, containing the recombinant bluescript plasmids

Autorzy:

Borowicz, B P

Powiązania:

https://bibliotekanauki.pl/articles/65003.pdf

Data publikacji:

1999

Wydawca:

Polska Akademia Nauk. Czytelnia Czasopism PAN

Tematy:

pET-3a vector
promoter system
cloning
I-18 C gene
T7 RNA polymerase
plasmid
Chironomus tentans
polymerase chain reaction
DNA fragment
identification

Opis:

The technology applied for the cloning of the open reading frame (ORF) I and II reflecting DNA fragments in regard to the identification of the bacterial colonies, of E. coli XL-1 strain, containing the recombinant bluescript plasmids, is described. The particular steps include: the α-complementation test, growing of the preliminary selected bacterial colonies in culture, isolation of the plasmids from these colonies, the Nde I and Bam HI digestion of the plamids mini-preparations, analysis of the digestion products and the identification of the bacterial colonies, containing the bluescript plasmids with the cloned inserts by the agarose gel electrophoresis (2% gel, 1 x TBE buffer).
Opisano technologię zastosowaną do klonowania fragmentów DNA odzwierciedlających otwartą ramę odczytu I i II genu I-18 C Chironomus tentans, w zakresie: identyfikacji kolonii bakteryjnych szczepu XL-1, zawierających zrekombinowane plazmidy blueskrypt. Poszczególne etapy obejmowały: test α-komplementacji, hodowlę wstępnie wybranych kolonii bakteryjnych, izolację plazmidów bakteryjnych poszczególnych kolonii z tych hodowli, trawienie mini-izolatów plazmidów Nde I i Bam HI, analizę produktów trawienia i identyfikację kolonii bakteryjnych, zawierających plazmidy blueskrypt z wklonowanymi wstawkami przy zastosowaniu elektroforezy w żelu agarozowym (2% żel, 1 x stężony bufor TBE).

Źródło:

Journal of Plant Protection Research; 1999, 39, 1
1427-4345

Pojawia się w:

Journal of Plant Protection Research

Dostawca treści:

Biblioteka Nauki

/5

Artykuł

Zmień widok

• na półce

Skocz do pozycji: 26.

Tytuł:

Cloning and expression of two DNA fregments of the I-18 C region of Chironomus tentans. I. The scheme of the performed experiments and the applied technology

Autorzy:

Borowicz, B P

Powiązania:

https://bibliotekanauki.pl/articles/65883.pdf

Data publikacji:

1998

Wydawca:

Polska Akademia Nauk. Czytelnia Czasopism PAN

Tematy:

promoter system
cloning
I-18 C gene
T7 RNA polymerase
Chironomus tentans
polymerase chain reaction
expression
pET vector
DNA fragment

Opis:

The I-18 C region of the polytenic chromosome of Chironomus tentans contains the I-18 C gene. Two DNA fragments, reflecting two different open reading frames (i.e.ORF I and II) of two different transcripts (i.e. 1.8 and 4.6 kb RNA) of the I-18 C gene were isolated, then were cloned into the intermediate vector and next to the final vector in order to translate them in T7 RNA polymerase / promoter system. The translation of the ORF I and II was performed to obtain the polypeptides of these ORFs for further study. Here, the scheme of the performed experiments and the applied technology that were necessary to realize the goal of this research, are presented.
Region i-18 C chromosomu politenicznego Chironomus tentans zawiera gen I-18 C. Dwa fragmenty DNA, odzwierciedlające dwie różne otwarte ramy odczytu (tj. ORF I i ORF II) dwóch różnych transkryptów (tj. 1.8 i 4.6 kpz RNA) genu I-18 C, zostały wyizolowane i następnie wklonowane do wektora pośredniego, a potem do wektora końcowego, w celu ich translacji w systemie promotora T7 RNA polimerazy. Translacja ORF I i ORF II była wykonana w celu uzyskania polipeptydów określonych przez te ORF do dalszych badań. W niniejszej pracy przedstawiono schemat wykonanych eksperymentów i zastosowaną technologię, która była potrzebna do zrealizowania celu badań.

Źródło:

Journal of Plant Protection Research; 1998, 38, 1
1427-4345

Pojawia się w:

Journal of Plant Protection Research

Dostawca treści:

Biblioteka Nauki

/5

Artykuł

Zmień widok

• na półce

Skocz do pozycji: 27.

Tytuł:

Antimicrobial activities and phylogenetic study of Erythrina senegalensis DC (Fabaceae) seed lectin

Autorzy:

Enoma, Samuel
Adewole, Taiwo S.
Agunbiade, Titilayo O.
Kuku, Adenike

Powiązania:

https://bibliotekanauki.pl/articles/16672367.pdf

Data publikacji:

2023

Wydawca:

Polska Akademia Nauk. Czasopisma i Monografie PAN

Tematy:

antimicrobial
Erythrina senegalensis
lectin
phylogenetic analysis
polymerase chain reaction

Opis:

Erythrina senegalensis (Fabaceae) have been traditionally used in the treatment of microbial ailments, and the specific agent mediating its efficacy has been investigated in several studies. In this study, the antimicrobial activity of purified E. senegalensis lectin (ESL) was analyzed. The phylogenetic relationship of the gene encoding lectin with other legume lectins was also established to investigate their evolutionary relationship via comparative genomics. Antimicrobial activity of ESL against selected pathogenic bacteria and fungi isolates was evaluated by the agar well diffusion method, using fluconazole (1 mg/ml) and streptomycin (1 mg/ml) as positive controls for fungi and bacteria sensitivity, respectively. Potent antimicrobial activity of ESL against Erwinia carotovora, Pseudomonas aeruginosa, Klebsiella pneumonia, Staphylococcus aureus, Aspergillus niger, Penicillium camemberti, and Scopulariopsis brevicaulis was observed, with inhibition zones ranging from 18 to 24 mm. Minimum inhibitory concentrations of ESL ranged between 50 and 400 μg/ml. Primer-directed polymerase chain reaction of E. senegalensis genomic DNA detected a 465-bp lectin gene with an open reading frame encoding a 134-amino acid polypeptide. The obtained nucleotide sequence of the ESL gene shared high sequence homology: 100, 100, and 98.18% with Erythrina crista-galli, Erythrina corallodendron, and Erythrina variegata lectin genes, respectively, suggesting that the divergence of Erythrina lectins might follow species evolution. This study concluded that ESL could be used to develop lectin-based antimicrobials, which could find applications in the agricultural and health sectors.

Źródło:

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2023, 104, 1; 21-32
0860-7796

Pojawia się w:

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

Dostawca treści:

Biblioteka Nauki

/5

Artykuł

Zmień widok

• na półce

Skocz do pozycji: 28.

Tytuł:

DNA isolation from Cryptosporidium oocysts directly from stool samples for diagnostic goals using PCR

Autorzy:

Sulima, P.

Powiązania:

https://bibliotekanauki.pl/articles/840154.pdf

Data publikacji:

1998

Wydawca:

Polskie Towarzystwo Parazytologiczne

Tematy:

diagnostic goal
stool
polymerase chain reaction
Cryptosporidium
DNA
oocyst

Źródło:

Annals of Parasitology; 1998, 44, 3
0043-5163

Pojawia się w:

Annals of Parasitology

Dostawca treści:

Biblioteka Nauki

/5

Artykuł

Zmień widok

• na półce

Skocz do pozycji: 29.

Tytuł:

Cloning of the DNA fragments reflecting the open reading frame I and II of the I-18 C gene of Chironomus tentans. III. Preparation of the inserts for ligation with the bluescript and the modification of the vector

Autorzy:

Borowicz, B P

Powiązania:

https://bibliotekanauki.pl/articles/66473.pdf

Data publikacji:

1999

Wydawca:

Polska Akademia Nauk. Czytelnia Czasopism PAN

Tematy:

cloning
pET-3a vector expression
I-18 C gene
T7 RNA polymerase
Chironomus tentans
promotor system
polymerase chain reaction
bluescript vector
DNA fragment

Opis:

The technology applied for the cloning of the open reading frame (ORF) I and II reflecting DNA fragments of the I-18 C gene of Chironomus tentans in regard to the preparation of the inserts for ligation with the bluescript and the modification of this intermediate vector, is described. The ORF I reflecting DNA fragment prepared for ligation with the bluescript vector, had one end blunt (i.e. the 5’ end with the Nde I restriction site) and the second end was sticky (i.e. the 3’ end after the Bam HI digestion of this insert). Subsequently, the bluescript vector for this ligation had also one end blunt (i.e. the 5’ end with the Nde I restriction site) and the second end was sticky (i.e. the 3’ end after the Bam HI digestion). The ORF II reflecting DNA fragment prepared for the ligation had both ends blunt and so the vector.
Opisano technologię zastosowaną do klonowania fragmentów DNA, odzwierciedlających translacyjną otwartą ramę odczytu I i II genu I-18 C w zakresie przygotowania wstawek do ligacji z plazmidem blueskrypt i niezbędnych modyfikacji wektora pośredniego. Fragment DNA odzwierciedlający otwartą ramę odczytu (ORF) I przygotowano do ligacji z wektorem blueskrypt w ten sposób, że fragment ten posiadał jeden koniec tępy (tj. koniec 5’ z miejscem restrykcyjnym dla Nde I) i drugi koniec lepki (tj. koniec 3’ po trawieniu tej wstawki Bam HI). Także wektor blueskrypt, użyty do ligacji ze wspomnianą sekwencją wstawki, miał jeden koniec tępy (tj. koniec 5’ z miejscem restrykcyjnym dla Nde I) i drugi koniec lepki (tj. koniec 3’ po trawieniu Bam HI). Fragment DNA odzwierciedlający ORF II, przygotowany do ligacji z wektorem blueskrypt, miał oba końce tępe i stosownie do tego oba końce wektora były też tępe.

Źródło:

Journal of Plant Protection Research; 1999, 39, 1
1427-4345

Pojawia się w:

Journal of Plant Protection Research

Dostawca treści:

Biblioteka Nauki

/5

Artykuł

Zmień widok

• na półce

Skocz do pozycji: 30.

Tytuł:

Cloning of the DNA fragments reflecting the open reading frame I and II of the I-18 C gene of Chironomus tentans. VI. The DNA sequencing of the cloned inserts

Autorzy:

Borowicz, B P

Powiązania:

https://bibliotekanauki.pl/articles/66720.pdf

Data publikacji:

1999

Wydawca:

Polska Akademia Nauk. Czytelnia Czasopism PAN

Tematy:

DNA sequence
pET-3a vector
promoter system
cloning
I-18 C gene
T7 RNA polymerase
Chironomus tentans
polymerase chain reaction
expression
DNA fragment

Opis:

The technology used for the cloning of the open reading frame (ORF) I and II reflecting DNA fragments in regard to the DNA sequencing of the cloned inserts, is presented. The object of the DNA sequencing of the cloned inserts, was to finally confirm and prove the sequence of the inserts as the sequence of the ORF I and II reflecting DNA fragments of the I-18 C gene of Chironomus tentans.
Przedstawiono technologię, zastosowaną do klonowania fragmentów DNA odzwierciedlających otwartą ramę odczytu I i II genu I-18 C Chironomus tentans w zakresie sekwencjonowania sklonowanych wstawek. Celem sekwencjonowania DNA sklonowanych wstawek było ostateczne potwierdzenie i udowodnienie, że sekwencje wklonowanych wstawek były sekwencjami fragmentów DNA odzwierciedlających otwartą ramę odczytu I i II genu I-18 C Chironomus tentans.

Źródło:

Journal of Plant Protection Research; 1999, 39, 1
1427-4345

Pojawia się w:

Journal of Plant Protection Research

Dostawca treści:

Biblioteka Nauki

/5

Artykuł

Zmień widok

• na półce

Skocz do pozycji: 31.

Tytuł:

Cloning of the DNA fragments reflecting the open reading frame I and II of the I-18 C gene of Chironomus tentans. I. Preparation of the bacterial cells, transformation and isolation of the bluescript plasmid

Autorzy:

Borowicz, B P

Powiązania:

https://bibliotekanauki.pl/articles/66849.pdf

Data publikacji:

1999

Wydawca:

Polska Akademia Nauk. Czytelnia Czasopism PAN

Tematy:

transformation
promoter system
gene expression
cloning
I-18 C gene
isolation
T7 RNA polymerase
plasmid
Chironomus tentans
bacterial cell
polymerase chain reaction
DNA fragment

Opis:

The technology applied for the cloning of the open reading frame (ORF) I and II reflecting DNA fragments of the I-18 C gene of Chironomus tentans in regard to the preparation of the competent E. coli cells of the XL-1 strain needed for the transformation with the ligation reaction products, is presented. Also the transformation of these bacterial cells with the bluescript plasmid and its isolation for these cloning experiments, are described.
Opisano technologię, którą zastosowano do klonowania fragmentów DNA odzwierciedlających translacyjną otwartą ramę odczytu I i II genu I-18 C Chironomus tentans w zakresie: przygotowania kompetentnych komórek E. coli szczepu XL-1, poddawanych następnie transformacji produktami reakcji ligacji. Przedstawiono także zastosowaną technologię transformacji wymienionych komórek bakteryjnych przy użyciu plazmidu - blueskrypt oraz izolację tego plazmidu, w celu jego zastosowania w wymienionych eksperymentach klonowania.

Źródło:

Journal of Plant Protection Research; 1999, 39, 1
1427-4345

Pojawia się w:

Journal of Plant Protection Research

Dostawca treści:

Biblioteka Nauki

/5

Artykuł

Zmień widok

• na półce

Skocz do pozycji: 32.

Tytuł:

A universal method for the identification of genes encoding amatoxins and phallotoxins in poisonous mushrooms

Autorzy:

Woloszyn, A.
Kotlowski, R.

Powiązania:

https://bibliotekanauki.pl/articles/875623.pdf

Data publikacji:

2017

Wydawca:

Narodowy Instytut Zdrowia Publicznego. Państwowy Zakład Higieny

Tematy:

identification method
gene encoding
amatoxin
phallotoxin
poisonous mushroom
polymerase chain reaction

Opis:

Background. As the currently known diagnostic DNA targets amplified in the PCR assays for detection of poisonous mushrooms have their counterparts in edible species, there is a need to design PCR primers specific to the genes encoding amanitins and phallotoxins, which occur only in poisonous mushrooms. Objective. The aim of the study was testing of PCR-based method for detection of all genes encoding hepatotoxic cyclic peptides - amanitins and phallotoxins present in the most dangerous poisonous mushrooms. Material and Methods. Degenerate primers in the PCR were designed on the basis of amanitins (n=13) and phallotoxins (n=5) genes in 18 species of poisonous mushrooms deposited to Genbank of the National Center for Biotechnology Information. Results. The specificity of the PCR assays was confirmed against 9 species of edible mushrooms, death cap - Amanita phalloides and panther cap - Amanita pantherina. Conclusions. Designed two couples of PCR-primers specific to amanitins and phallotoxins genes can be recommended for detection of Amanita phalloides and other mushroom species producing hepatotoxic cyclic peptides - amanitins and phallotoxins.
Wprowadzenie. Ponieważ, obecnie znane diagnostyczne cele molekularne w genomach trujących grzybów kapeluszowych amplifikowane metodą PCR mają swoje odpowiedniki u grzybów jadalnych, istnieje potrzeba zastosowania specyficznych sekwencji starterowych wobec amanityn i fallotoksyn, występujących jedynie u grzybów trujących. Cel. Celem prowadzonych badań było sprawdzenie przydatności sekwencji starterowych do reakcji PCR specyficznych wobec wszystkich aktualnie poznanych genów kodujących hepatotoksyczne cykliczne peptydy - amanityny oraz fallotoksyny trujących grzybów kapeluszowych. Materiał i Metody. Sekwencje oligonokleotydowe starterów do reakcji PCR zaprojektowane zostały w oparciu o zdeponowane w Genbanku geny amanityn (n=13) oraz fallotoksyn (n=5). Wyniki. Specyficzność opracowanych testów PCR potwierdzono wobec 9 gatunków grzybów jadalnych oraz muchomora sromotnikowego - Amanita phalloides, jak i muchomora plamistego - Amanita pantherina. Wnioski. Zastosowane sekwencje starterowe do wykrywania genów kodujących amanityny i fallotoksyny metodą PCR, mogą być wykorzystane do wykrywania muchomora sromotnikowego oraz innych gatunków zdolnych do syntezy amanityn oraz fallotoksyn.

Źródło:

Roczniki Państwowego Zakładu Higieny; 2017, 68, 3
0035-7715

Pojawia się w:

Roczniki Państwowego Zakładu Higieny

Dostawca treści:

Biblioteka Nauki

/5

Artykuł

Zmień widok

• na półce

Skocz do pozycji: 33.

Tytuł:

Principles and applications of Ligation Mediated PCR methods for DNA-based typing of microbial organisms

Autorzy:

Krawczyk, Beata
Kur, Józef
Stojowska-Swędrzyńska, Karolina
Śpibida, Marta

Powiązania:

https://bibliotekanauki.pl/articles/1038839.pdf

Data publikacji:

2016

Wydawca:

Polskie Towarzystwo Biochemiczne

Tematy:

rapid methods
molecular epidemiology
genotyping
microbial phylogenetics
PCR (polymerase chain reaction)

Opis:

A significant number of DNA-based techniques has been introduced into the field of microorganisms' characterization and taxonomy. These genomic fingerprinting methods were developed to detect DNA sequence polymorphisms by using general principles, such as restriction endonuclease analysis, molecular hybridization, and PCR amplification. In recent years, some alternative techniques based on ligation of oligonucleotide adapters before DNA amplification by PCR, known as Ligation-Mediated PCR methods (LM PCR), have been successfully applied for the typing of microorganisms below the species level. These molecular methods include: Amplified Fragment Length Polymorphism (AFLP), Amplification of DNA fragments Surrounding Rare Restriction Sites (ADSRRS), PCR Melting Profiles (PCR MP), Ligation Mediated PCR/Shifter (LM PCR/Shifter), Infrequent-Restriction-Site Amplification (IRS PCR), double digestion Ligation Mediated Suppression PCR (ddLMS PCR). These techniques are now applied more and more often because they involve less time, are comparably inexpensive, and require only standard lab equipment. Here, we present a general review of this group of methods showing their possibilities and limitations. We also identify questions and propose solutions which may be helpful in choosing a particular LM PCR method for the achievement of the required goal.

Źródło:

Acta Biochimica Polonica; 2016, 63, 1; 39-52
0001-527X

Pojawia się w:

Acta Biochimica Polonica

Dostawca treści:

Biblioteka Nauki

/5

Artykuł

Zmień widok

• na półce

Skocz do pozycji: 34.

Tytuł:

Isolation of the DNA fragment reflecting the open reading frame II of the I-18 C gene of Chironomus tentans by the polymerase chain reaction. IV. The product of the applied technologies

Autorzy:

Borowicz, B P

Powiązania:

https://bibliotekanauki.pl/articles/65915.pdf

Data publikacji:

1997

Wydawca:

Polska Akademia Nauk. Czytelnia Czasopism PAN

Tematy:

I-18 C gene
Chironomus tentans
polymerase chain reaction
DNA fragment

Opis:

The Polymerase Chain Reaction and other molecular technologies were applied to isolate the DNA fragment reflecting the Open Reading Frame II of the I-18 C gene. This research object was realized and the importance of this result has been discussed in view of the mechanisms of the regulation of gene expression.
Reakcja Łańcuchowa Polimerazy i inne technologie molekularne zostały zastosowane w celu izolacji fragmentu DNA odpowiadającego Otwartej Ramie Odczytu II genu I-18 C. Cel badawczy został zrealizowany i omówiono znaczenie tego wyniku w świetle mechanizmów regulacji ekspresji genu.

Źródło:

Journal of Plant Protection Research; 1997, 37, 1-2
1427-4345

Pojawia się w:

Journal of Plant Protection Research

Dostawca treści:

Biblioteka Nauki

/5

Artykuł

Zmień widok

• na półce

Skocz do pozycji: 35.

Tytuł:

Cloning of the DNA fragments reflecting the open reading frame I and II of the I-18 C gene of Chironomus tentans. VIII. The ligations of the inserts with the pET-3a vector and the identification of the bacterial colonies, containing the recombinant plasmids

Autorzy:

Borowicz, B P

Powiązania:

https://bibliotekanauki.pl/articles/66584.pdf

Data publikacji:

1999

Wydawca:

Polska Akademia Nauk. Czytelnia Czasopism PAN

Tematy:

pET-3a vector
promoter system
cloning
I-18 C gene
T7 RNA polymerase
Chironomus tentans
polymerase chain reaction
expression
DNA fragment
recombinant plasmid
identification

Opis:

The technology used for the cloning of the open reading frame (ORF) I and II reflecting DNA fragments in regard to the ligations of the inserts with the pET-3a vector and the identification of the bacterial colonies, containing the recombinant plasmids, is presented. The main steps include: calculation of the molar ratios of the vector to insert DNA, the ligation reactions, transformation of the competent E. coli cells of the XL-1 strain, isolation of the plasmids, digestion of the plasmid preparations with the Nde I and Bam HI to indicate the presence of the inserts cloned into the plasmids, using the agarose gel electrophoresis (2% gel, 1 x TBE buffer) of the plasmids samples, with the released inserts, after the digestion, transformation of the competent BL21 (DE3) cells of E. coli with the isolated recombinant plasmids, identification of the bacterial cells, carrying the pET-3a plasmids with the cloned inserts and the storage of these cells.
Przedstawiono technologię użytą do klonowania fragmentów DNA odzwierciedlających otwartą ramę odczytu I i II genu I-18 C Chironomus tentans w zakresie ligacji wstawek z wektorem pET-3a oraz identyfikacji kolonii bakteryjnych, zawierających rekombinacyjne plazmidy. Główne etapy obejmują: obliczenie stosunków molarnych wektora do DNA wstawki, reakcje ligacji, transformację komórek kompetentnych E. coli szczepu XL-1, izolację plazmidów, trawienie preparatów plazmidów Nde I i Bam Hl w celu wskazania obecności wklonowanych do plazmidów wstawek, uwolnionych z tych plazmidów poprzez trawienie wymienionymi enzymami restrykcyjnymi. Analizę produktów trawienia poszczególnych próbek plazmidów wykonano przy użyciu elektroforezy w żelu agarozowym (2% żel, 1 x stężony bufor TBE). Następne etapy to transformacja komórek kompetentnych E. coli BL21(DE3) preparatami wyizolowanych zrekombinowanych plazmidów pET-3a i identyfikacja komórek bakteryjnych, zawierających plazmidy pET-3a z wklonowanymi wstawkami oraz przechowywanie tych komórek.

Źródło:

Journal of Plant Protection Research; 1999, 39, 1
1427-4345

Pojawia się w:

Journal of Plant Protection Research

Dostawca treści:

Biblioteka Nauki

/5

Artykuł

Zmień widok

• na półce

Skocz do pozycji: 36.

Tytuł:

Cloning of the DNA fragments reflecting the open reading frame I and II of the I-18 C gene of Chironomus tentans. IV. The ligation of the bluescript vector with the inserts

Autorzy:

Borowicz, B P

Powiązania:

https://bibliotekanauki.pl/articles/65392.pdf

Data publikacji:

1999

Wydawca:

Polska Akademia Nauk. Czytelnia Czasopism PAN

Tematy:

pET-3a vector
promoter system
cloning
I-18 C gene
T7 RNA polymerase
Chironomus tentans
polymerase chain reaction
expression
DNA fragment

Opis:

The technology used for the cloning of the open reading frame (ORF) I and II reflecting DNA fragments of the I-18 C gene in regard to the ligation reactions of the bluescript vector with the inserts, is presented. The main steps include: the calculation of the molar ratio of the bluescript plasmid to the ORF I and II reflecting DNA fragments as the inserts, the ligation reaction, the transformation of the E. coli cells of the XL- 1 strain with the ligation reactions products and growing of the transformed bacterial cells.
Przedstawiono technologię użytą do klonowania fragmentów DNA odzwierciedlających otwartą ramę odczytu I i II genu I-18 C Chironomus tentans w zakresie: reakcji ligacji wektora blueskrypt ze wstawkami sekwencji klonowanych wymienionych wyżej. Przedstawione główne etapy obejmują: obliczenie stosunku molarnego plazmidu blueskrypt do fragmentu DNA odzwierciedlającego otwartą ramę odczytu I i II. Fragmenty te zostały użyte jako wstawki. Przedstawiono także zastosowaną technologię dotyczącą reakcji ligacji, transformacji komórek bakteryjnych E. coli szczepu XL-1 produktami reakcji ligacji oraz hodowlę na płytkach agarowych stransformowanych komórek bakterii.

Źródło:

Journal of Plant Protection Research; 1999, 39, 1
1427-4345

Pojawia się w:

Journal of Plant Protection Research

Dostawca treści:

Biblioteka Nauki

/5

Artykuł

Zmień widok

• na półce

Skocz do pozycji: 37.

Tytuł:

Cloning of the DNA fragments reflecting the open reading frame I and II of the I-18 C gene of Chironomus tentans. VII. The preparation of the inserts and the translational vector - pET-3a for ligation

Autorzy:

Borowicz, B P

Powiązania:

https://bibliotekanauki.pl/articles/66798.pdf

Data publikacji:

1999

Wydawca:

Polska Akademia Nauk. Czytelnia Czasopism PAN

Tematy:

pET-3a vector
promoter system
cloning
I-18 C gene
T7 RNA polymerase
Chironomus tentans
polymerase chain reaction
expression
DNA fragment

Opis:

The technology applied for the cloning of the open reading frame (ORF) I and II reflecting DNA fragments in regard to the preparation of the inserts and the translational vector pET-3a for ligation, is described. The main steps of the preparation of the inserts include: digestion of the isolated recombinant bluescript plasmids, carrying the inserts, with the Nde I and Bam HI, purification and isolation of the inserts after the digestion, by the preparative agarose gel electrophoresis (2% gel, 1 x TAE buffer) and then by the centrifugation through the filtration device of the isolated DNA fragment from the gel and also estimation of the inserts concentration by the agarose gel electrophoresis (2% gel, 1 x TBE buffer). The main steps of the preparation of the pET-3a vector included: transformation of the competent E. coli cells of the XL-1 strain with the pET-3a, growing of the transformed bacteria on agar plates (with ampicillin) and then growing of the isolated transformed bacterial colonies in culture to finally isolate the plasmid, digestion of the plasmid with the Nde I and Bam HI, purification of the isolatcd plasmid, after the digestion, by the preparative agarose gel electrophoresis (0.8% gel, 1 x TAE buffer), centrifugation through the filtration device of the isolated DNA fragment from the gel, phenol extraction of the isolated plasmid, estimation of the concentration of the plasmid by the agarose gel electrophoresis (0.8% gel, 1 x TBE buffer).
Opisano technologię zastosowaną do klonowania fragmentów DNA odzwierciedlających otwartą ramę odczytu I i II w zakresie przygotowania wstawek i wektora translacyjnego - pET-3a do ligacji. Główne etapy przygotowania wstawek obejmowały: - trawienie Nde I i Bam HI, wyizolowanych rekombinacyjnych plazmidów blueskrypt, które zawierały wstawki, - oczyszczanie i izolację wstawek, po trawieniu przy użyciu preparatywnej elektroforezy w żelu agarozowym (2% żel, 1 x stężony bufor TAE) i następnie przez odwirowanie wyizolowanego fragmentu DNA z żelu, przy użyciu urządzenia filtracyjnego - oraz ocenę stężenia wstawek przy użyciu elektroforezy (2% żel, 1 x stężony bufor TBE). Główne etapy przygotowania wektora pET-3a obejmowały: transformację komórek kompetentnych E. coli szczepu XL-1, wektorem pET-3a, hodowlę stransformowanych komórek bakteryjnych na płytkach agarowych (z ampicyliną) i następnie hodowlę w kulturze wyizolowanych stransformowanych komórek bakteryjnych w celu izolacji plazmidu, trawienie wyizolowanego plazmidu Nde I i Bam HI oraz jego oczyszczenie po trawieniu, przy użyciu preparatywnej elektroforezy w żelu agarozowym (0.8% żel, 1 x stężony bufor TAE), odwirowanie wyodrębnionego z żelu fragmentu DNA przy zastosowaniu urządzenia filtracyjnego, ekstrakcję fenolową wyizolowanego plazmidu, ocenę stężenia plazmidu przy użyciu elektroforezy w żelu agarozowym (0.8% żel, 1 x stężony TBE).

Źródło:

Journal of Plant Protection Research; 1999, 39, 1
1427-4345

Pojawia się w:

Journal of Plant Protection Research

Dostawca treści:

Biblioteka Nauki

/5

Artykuł

Zmień widok

• na półce

Skocz do pozycji: 38.

Tytuł:

SYBR Green-based real-time polymerase chain reaction assay for detection of porcine parvovirus 6 in pigs

Autorzy:

Sun, P.
Bai, C.X.
Zhang, D.
Wang, J.
Yang, K.K.
Cheng, B.Z.
Li, Y.D.
Wang, Y.

Powiązania:

https://bibliotekanauki.pl/articles/2087328.pdf

Data publikacji:

2020

Wydawca:

Polska Akademia Nauk. Czytelnia Czasopism PAN

Tematy:

porcine parvovirus 6
real-time polymerase chain reaction
SYBR Green

Źródło:

Polish Journal of Veterinary Sciences; 2020, 23, 2; 197-202
1505-1773

Pojawia się w:

Polish Journal of Veterinary Sciences

Dostawca treści:

Biblioteka Nauki

/5

Artykuł

Zmień widok

• na półce

Skocz do pozycji: 39.

Tytuł:

Quantification of thioredoxin mRNA expression in the rat hippocampus by real-time PCR following oxidative stress.

Autorzy:

Yalcin, Ayfer

Powiązania:

https://bibliotekanauki.pl/articles/1041524.pdf

Data publikacji:

2004

Wydawca:

Polskie Towarzystwo Biochemiczne

Tematy:

kainic acid
real-time polymerase chain reaction
oxidative stress
thioredoxin

Opis:

Thioredoxin (Trx) is a multifunctional protein with a redox-active disulfide/dithiol in the active site. Thioredoxin, with its redox-regulating and reactive oxygen species (ROS) scavenging activities, plays several important biologic roles both in intracellular and extracellular compartments. The purpose of this report was to quantify the relative expression of Trx in rat hippocampus following an oxidative stress-involving treatment such as kainic acid (KA) using real-time PCR and the 2-ΔΔCT method. The relative changes in expression of Trx mRNA in KA-treated and control animals were significantly different as 2.02 ± 0.77 and 1.0 ± 0.26, respectively ( P <0.05). Minimum and maximum n-fold changes in Trx expression in KA-treated and control animals were determined as (1.4-5.2) and (0.8-1.3), respectively. Thus, real-time PCR and the 2-ΔΔCT method for data analysis from real-time PCR were found to be an accurate and sensitive method for quantifying Trx mRNA levels.

Źródło:

Acta Biochimica Polonica; 2004, 51, 4; 1059-1065
0001-527X

Pojawia się w:

Acta Biochimica Polonica

Dostawca treści:

Biblioteka Nauki

/5

Artykuł

Zmień widok

• na półce

Skocz do pozycji: 40.

Tytuł:

Quantification of thioredoxin mRNA expression in the rat hippocampus by real-time PCR following oxidative stress.

Autorzy:

Yalcin, Ayfer

Powiązania:

https://bibliotekanauki.pl/articles/1041529.pdf

Data publikacji:

2004

Wydawca:

Polskie Towarzystwo Biochemiczne

Tematy:

kainic acid
real-time polymerase chain reaction
oxidative stress
thioredoxin

Opis:

Thioredoxin (Trx) is a multifunctional protein with a redox-active disulfide/dithiol in the active site. Thioredoxin, with its redox-regulating and reactive oxygen species (ROS) scavenging activities, plays several important biologic roles both in intracellular and extracellular compartments. The purpose of this report was to quantify the relative expression of Trx in rat hippocampus following an oxidative stress-involving treatment such as kainic acid (KA) using real-time PCR and the 2-ΔΔCT method. The relative changes in expression of Trx mRNA in KA-treated and control animals were significantly different as 2.02 ± 0.77 and 1.0 ± 0.26, respectively ( P <0.05). Minimum and maximum n-fold changes in Trx expression in KA-treated and control animals were determined as (1.4-5.2) and (0.8-1.3), respectively. Thus, real-time PCR and the 2-ΔΔCT method for data analysis from real-time PCR were found to be an accurate and sensitive method for quantifying Trx mRNA levels.

Źródło:

Acta Biochimica Polonica; 2004, 51, 4; 1087-1090
0001-527X

Pojawia się w:

Acta Biochimica Polonica

Dostawca treści:

Biblioteka Nauki

/5

Artykuł

Zmień widok

• na półce

Skocz do pozycji: 41.

Tytuł:

Virulence and antibiotic resistance of Escherichia coli isolated from rooks

Autorzy:

Kmet, V.
Drugdova, Z.
Kmetova, M.
Stanko, M.

Powiązania:

https://bibliotekanauki.pl/articles/51141.pdf

Data publikacji:

2013

Wydawca:

Instytut Medycyny Wsi

Tematy:

virulence
antibiotic resistance
Escherichia coli
isolation
rook
polymerase chain reaction
DNA microarray

Opis:

With regard to antibiotic resistance studies in various model animals in the urban environment, the presented study focused on the rook, many behavioural and ecological aspects of which are important from an epidemiological point of view. A total of 130 Escherichia coli strains isolated from rook faeces during a two-year period (2011–2012) were investigated for antibiotic resistance and virulence. Resistance to ampicillin (60%) and streptomycin (40%) were the most frequent, followed by resistance to fluoroquinolones (ciprofloxacin-22% and enrofloxacin-24%), tetracycline (18%), cotrimoxazol (17%) and florfenicol (14%). Ceftiofur resistance occured in 10.7 % of strains and cefquinom resistance in 1.5 % of strains. Twenty-five E.coli strains with a higher level of MICs of cephalosporins (over 2mg/L of ceftazidime and ceftriaxon) and fluoroquinolones were selected for detection of betalactamase genes (CTX-M, CMY), plasmid-mediated quinolone resistance qnrS, integrase 1, and for APEC (avian pathogenic E.coli) virulence factors (iutA, cvaC, iss, tsh, ibeA, papC, kpsII). Genes of CTX-M1, CMY-2, integrase 1, papC, cvaC, iutA were detected in one strain of E.coli, and qnrS, integrase 1, iss, cvaC, tsh were detected in another E.coli. DNA microarray revealed the absence of verotoxin and enterotoxin genes and pathogenicity islands. The results show that rooks can serve as a reservoir of antibiotic-resistant E. coli with avian pathogenic virulence factors for the human population, and potentially transmit such E.coli over long distances.

Źródło:

Annals of Agricultural and Environmental Medicine; 2013, 20, 2
1232-1966

Pojawia się w:

Annals of Agricultural and Environmental Medicine

Dostawca treści:

Biblioteka Nauki

/5

Artykuł

Zmień widok

• na półce

Skocz do pozycji: 42.

Tytuł:

Segmented hybridization probes: modulating target affinity and base pairing selectivity

Autorzy:

Egetenmeyer, S.
Geiger, E.
Richert, C.

Powiązania:

https://bibliotekanauki.pl/articles/81071.pdf

Data publikacji:

2012

Wydawca:

Polska Akademia Nauk. Czytelnia Czasopism PAN

Tematy:

oligonucleotide
DNA
RNA
hybridization
base pairing
molecular biology
polymerase chain reaction

Źródło:

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2012, 93, 3
0860-7796

Pojawia się w:

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

Dostawca treści:

Biblioteka Nauki

/5

Artykuł

Zmień widok

• na półce

Skocz do pozycji: 43.

Tytuł:

Polymorphism of DNA in nematodes of genus Trichinella Railliet, 1895 according to the data of polymerase chain reaction

Autorzy:

Shendrick, A.
Benedictov, I.
Bessonov, A.

Powiązania:

https://bibliotekanauki.pl/articles/838885.pdf

Data publikacji:

1998

Wydawca:

Polskie Towarzystwo Parazytologiczne

Tematy:

polymorphism
Trichinella pseudospiralis
Trichinella
nematode
polymerase chain reaction
DNA
Trichinella spiralis

Źródło:

Annals of Parasitology; 1998, 44, 3
0043-5163

Pojawia się w:

Annals of Parasitology

Dostawca treści:

Biblioteka Nauki

/5

Artykuł

Zmień widok

• na półce

Skocz do pozycji: 44.

Tytuł:

Drummondin E and Flinderole B are potential inhibitors of RNA-dependent RNA polymerase of SARS-CoV-2: an in silico study

Autorzy:

Akhtar, N.
Verma, H.
Silkari, O.M.
Upadhyay, A.K.
Kaushik, V.
Mannan, M.A.

Powiązania:

https://bibliotekanauki.pl/articles/2096249.pdf

Data publikacji:

2022

Wydawca:

Polska Akademia Nauk. Czytelnia Czasopism PAN

Tematy:

RNA polymerase
SARS-CoV-2
RNA-dependent
Drummondin E
Flinderole B

Opis:

Coronavirus disease 2019 caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has affected 235.6 million people worldwide. In the present study, RNA-dependent RNA polymerase (RdRp) (PDB Id: 6M71) of SARS-CoV-2, an essential enzyme needed for subgenomic replication and amplification of RNA, was selected. Similar to other RdRps, it is a conserved protein and a popular target for antiviral drug therapy. Based on a computational approach, potential RdRp inhibitors were identified. The absorption, distribution, metabolism, excretion, and toxicity (ADMET) of selected molecules were determined using computation tools. The potential inhibitors were docked to the RdRp and later confirmed by Molecular Dynamics (MD) using the “Flare” module of Cresset software. Drummondin E and Flinderole B had higher drug similarity scores among the compounds selected in this study. Both these compounds are noncarcinogenic, nonirritant, nontumorigenic, and nonmutagenic. Molecular docking studies showed that both compounds can bind to RdRp. The best ligand interaction patterns were validated by MD using the “Flare” module. MD was performed for the period of 100 ns with the time step of 1 fs. The simulation results suggest that Thr-680, Arg-624, Lys-676, and Val-557 are key interacting partners in the Drummondin E-RdRp complex, while Asp-618, Asp-760, Asp-623, Arg-624, and Asp-761 are the interacting partners in the Flinderole B-RdRp complex. Based on the in silico drug-likeness score; ADMET properties; and molecular simulation result, we surmise that Flinderole B and Drummondin E could impede SARS-CoV-2 genome replication and transcription by targeting the RdRp protein.

Źródło:

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2022, 103, 1; 53-70
0860-7796

Pojawia się w:

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

Dostawca treści:

Biblioteka Nauki

/5

Artykuł

Zmień widok

• na półce

Skocz do pozycji: 45.

Tytuł:

Acute SARS-CoV-2 infection and seropositivity among healthcare workers and medical students in summer 2020, Hungary

Autorzy:

Kosztin, Annamária
Merkely, Béla
Szabó, Attila J.
Blaha, Béla
Varga, Péter
Vásárhelyi, Barna
Vokó, Zoltán

Powiązania:

https://bibliotekanauki.pl/articles/2084806.pdf

Data publikacji:

2022-04-11

Wydawca:

Instytut Medycyny Pracy im. prof. dra Jerzego Nofera w Łodzi

Tematy:

polymerase chain reaction
medical students
seropositivity
COVID-19
SARS-CoV-2
healthcare workers

Opis:

ObjectivesThe aim was to compare the prevalence of acute infection and seropositivity of SARS-CoV-2 among healthcare workers (HCWs) and medical students.Material and MethodsA high-volume, single-center analysis was conducted in the period of July 1‒August 1, 2020, at the Semmelweis University. Naso- and oropharyngeal samples were collected for polymerase chain reaction (PCR), and blood samples for anti-SARS-CoV-2 IgG. A questionnaire was also administered about the infection symptoms and the obtained results were assessed by profession and site of care delivery.ResultsFrom the total cohort (N = 7948), 4478 (56%) and 3470 (44%) were health professionals and medical students, respectively. They were mainly female (67%), and the mean age of HCWs and students was 40 and 25 years, respectively. By profession, physicians (1.5%) and other HCWs (1.8%) showed a comparable SARS-CoV-2 exposure. International students had the highest (2.1%), whereas Hungarian students had the lowest (0.6%) prevalence of seropositivity. The highest prevalence was detected among the staff of COVID-19 wards (12.1%). By PCR, medical students showed the lowest occurrence of active infection with a prevalence of 0.17%, while physicians and other HCWs had a higher prevalence (1.46% and 1.71%, respectively). By site of care delivery, positive test results were the most frequent at COVID-19 wards (3.8%).ConclusionsPhysicians and other HCWs showed comparable SARS-CoV-2 seropositivity prevalence, approximately twice as high as in the general population of Budapest. Hungarian students had lower prevalence of seropositivity than this reference. High prevalence among international students suggests that they had imported the infection. The very high prevalence of documented exposure among staff members at COVID-19 wards urges for improving the safety measures.

Źródło:

International Journal of Occupational Medicine and Environmental Health; 2022, 35, 2; 209-216
1232-1087
1896-494X

Pojawia się w:

International Journal of Occupational Medicine and Environmental Health

Dostawca treści:

Biblioteka Nauki

/5

Artykuł

Zmień widok

• na półce

Skocz do pozycji: 46.

Tytuł:

Poly(adP-ribose) polymerase inhibitor olaparib in the treatment of ovarian cancer: a comprehensive review of current literature

Autorzy:

Poboży, Kamil
Domańska, Julia
Domański, Paweł

Powiązania:

https://bibliotekanauki.pl/articles/22792529.pdf

Data publikacji:

2023-07-02

Wydawca:

Medical Education

Tematy:

Olaparib
PARP inhibitor
ovarian cancer
targeted therapy
poly(ADP-ribose) polymerase
cancer therapy

Opis:

Purpose of the review: This comprehensive review aims to provide a summary of current research on the utilization of olaparib, a poly(ADP-ribose) polymerase (PArP) inhibitor, in the treatment of ovarian cancer. The review aims to highlight the key findings from recent clinical trials and assess the potential of olaparib as a targeted therapy for improving the prognosis of ovarian cancer patients. recent findings: Ovarian cancer remains a significant global health concern with high mortality rates. While optimal debulking surgery and platinum-based chemotherapy are the standard treatments, the recurrence rates remain substantial. The emergence of PArP inhibitors, particularly olaparib, has intro duced a novel therapeutic approach that targets the genomic instability and DnA repair mechanisms in cancer cells. notable clinical trials, such as SOl O1, SOl O2, and PAOlA-1, have demonstrated the effectiveness of olaparib in significantly improving progression-free survival, particularly in patients with Br CA mutations or homologous recombination deficiency. Additionally, combination therapies involving olaparib, such as those with bevacizumab or entinostat, have shown promising results. Summary: The utilization of olaparib has brought about a paradigm shift in the treatment of ovarian cancer. notably, it has shown significant improvements in progression-free survival and overall survival, particularly in patients with Br CA mutations or homologous recombination deficiency. The exploration of olaparib through various clinical trials and combination therapies continues to provide valuable insights and offer new prospects for ovarian cancer patients. Moreover, the growing understanding of PArP inhibitors holds the potential for further advancements in the prognosis of patients with this formidable condition.

Źródło:

OncoReview; 2023, 13, 2; 39-47
2450-6125

Pojawia się w:

OncoReview

Dostawca treści:

Biblioteka Nauki

/5

Artykuł

Zmień widok

• na półce

Skocz do pozycji: 47.

Tytuł:

Optimization of the Y831C mutation detection in human DNA polymerase gamma by allelic discrimination assay

Autorzy:

Stopińska, Katarzyna
Grzybowski, Tomasz
Malyarchuk, Boris
Derenko, Miroslava
Miścicka-Śliwka, Danuta

Powiązania:

https://bibliotekanauki.pl/articles/1041222.pdf

Data publikacji:

2006

Wydawca:

Polskie Towarzystwo Biochemiczne

Tematy:

real-time PCR
polymerase γ
progressive external ophthalmoplegia
single nucleotide polymorphisms (SNPs)

Opis:

Many well-defined mutations in the gene for the catalytic subunit of polymerase γ (POLG1) have been found to be associated with disease, whereas the status of several mutations remains unresolved due to the conflicting reports on their frequencies in populations of healthy individuals. Here, we have developed a highly sensitive, real-time allelic discrimination assay enabling detection of the Y831C mutation in the POLG1 gene. The Y831C mutation is present in the Polish population at a frequency of 2.25%. The new assay is well suited to both extensive population studies and molecular diagnostics of POLG1.

Źródło:

Acta Biochimica Polonica; 2006, 53, 3; 591-595
0001-527X

Pojawia się w:

Acta Biochimica Polonica

Dostawca treści:

Biblioteka Nauki

/5

Artykuł

Zmień widok

• na półce

Skocz do pozycji: 48.

Tytuł:

Detection of nivalenol and deoxynivalenol chemotypes produced by Fusarium graminearum species complex isolated from barley in Iran using specific PCR assays

Autorzy:

Chehri, K.
Godini, R.

Powiązania:

https://bibliotekanauki.pl/articles/66014.pdf

Data publikacji:

2017

Wydawca:

Polska Akademia Nauk. Czytelnia Czasopism PAN

Tematy:

detection
nivalenol
deoxynivalenol
Fusarium graminearum
isolation
polymerase chain reaction
barley
trichothecene
Iran

Opis:

In order to identify trichothecenes chemotypes produced by Fusarium graminearum species complex (FGSC) isolated from barley, 68 barley samples were collected from markets in Kermanshah and Hamedan provinces, Iran. Thirty-one Fusarium isolates were obtained from grains and morphologically classified into three species FGSC (14), F. equiseti (9), and F. proliferatum (8). The identification of the members of FGSC was confirmed molecularly using Fg16F/Fg16R primers. Fusarium asiaticum isolates (4) were distinguished from other FGSC using Fg6CTPSf177/Fg16R primers. Polymerase chain reaction-based (PCRbased) detection of mycotoxin-synthesis-pathway gene was also used to determine the potential of the analysed strains to produce deoxynivalenol (DON), 15-acetyldeoxynivalenol (15-AcDON), 3-acetyldeoxynivalenol (3-AcDON), and nivalenol (NIV). Of 14 tested isolates, 10 and 4 isolates belonged to DON and NIV chemotype, respectively. Also, the results of DON chemotype survey using specific primers MinusTri7F/R and Tri315F/R showed 1 and 9 isolates produced 3-AcDON and 15-AcDON, respectively. These results show that DON was the most common chemotype in western Iran. To our knowledge, this is the first report on 15-AcDON, 3-AcDON, and NIV isolated from barley in Iran.

Źródło:

Journal of Plant Protection Research; 2017, 57, 3
1427-4345

Pojawia się w:

Journal of Plant Protection Research

Dostawca treści:

Biblioteka Nauki

/5

Artykuł

Zmień widok

• na półce

Skocz do pozycji: 49.

Tytuł:

Comparison between two approaches to modeling microsatellite DNA repeats: infinite dimensional model and its n-dimensional

Autorzy:

Białka, M.

Powiązania:

https://bibliotekanauki.pl/articles/229939.pdf

Data publikacji:

2011

Wydawca:

Polska Akademia Nauk. Czytelnia Czasopism PAN

Tematy:

infinite-dimensional systems
asymptotic stability
chain systems
microsatellites DNA repeats
polymerase slippage

Opis:

Two approaches to modeling microsatellite DNA repeats are considered. The former is an infinite dimensional system based on the theory of branching random walks which dynamic properties are characterized using Laplace transforms and Laplace asymptotic techniques. The latter is an n-dimensional approximation where microsatellite DNA repeats model is the example of a chain system. Both models were the subject of many numerical calculations using the MATLAB software. The results allow us to evaluate the asymptotic behavior and determine the effect of the system parameters on the run of the solution and the state variables.

Źródło:

Archives of Control Sciences; 2011, 21, 4; 419-441
1230-2384

Pojawia się w:

Archives of Control Sciences

Dostawca treści:

Biblioteka Nauki

/5

Artykuł

Zmień widok

• na półce

Skocz do pozycji: 50.

Tytuł:

Wild game as a reservoir of Anaplasma phagocytophilum in North-Western Poland

Autorzy:

Adamska, M
Skotarczak, B.

Powiązania:

https://bibliotekanauki.pl/articles/840225.pdf

Data publikacji:

2007

Wydawca:

Polskie Towarzystwo Parazytologiczne

Tematy:

parasite
Polska
reservoir
roe deer
bacteria
Anaplasma phagocytophilum
polymerase chain reaction
intracellular parasite
tick

Źródło:

Annals of Parasitology; 2007, 53, 2
0043-5163

Pojawia się w:

Annals of Parasitology

Dostawca treści:

Biblioteka Nauki

/5

Artykuł

Zmień widok

• na półce

Skocz do pozycji: 51.

Tytuł:

Influence of 137Cs and 90Sr on vegetative and generative organs of Lepidium sativum L. and Tradescantia clone 02

Autorzy:

Marciulioniene, D.
Lukšiene, B.
Kiponas, D.
Montvydiene, D.
Maksimov, G.
Darginaviciene, J.
Gaveliene, V.

Powiązania:

https://bibliotekanauki.pl/articles/146171.pdf

Data publikacji:

2006

Wydawca:

Instytut Chemii i Techniki Jądrowej

Tematy:

technogenic radionuclides
activity concentration
accumulation
Tradescantia clone BNL 02
Lepidium sativum
RNA-polymerase II

Opis:

The impact of 137Cs and 90Sr activity concentrations from 0.4 to 400 kBqźL-1 and from 1 to 200 kBqźL-1, respectively, on seed germination of Lepidium sativum was insignificant; however, all concentrations of 137Cs and 30 kBqźL-1 concentration of 90Sr slightly stimulated root growth. The accumulated 137Cs and 90Sr stimulated shoot and parenchyma cell growth; 137Cs suppressed RNA-polymerase II activity, and 90Sr, on the contrary, stimulated it. Different genotoxic effects on Tradescantia clone 02 stamen hair cells were observed with comparable 137Cs and 90Sr activity concentrations. Activity concentrations of 137Cs from 0.001 to 1.3 kBqźL-1 were more effective in Tradescantia clone 02 stamen hair cell reproduction, whereas the studied activity concentrations of 90Sr (from 0.002 to 640 kBqźL-1) induced more mutations. --------------------------------------------------------------------------------

Źródło:

Nukleonika; 2006, 51, 4; 193-201
0029-5922
1508-5791

Pojawia się w:

Nukleonika

Dostawca treści:

Biblioteka Nauki

/5

Artykuł

Zmień widok

• na półce

Skocz do pozycji: 52.

Tytuł:

Familial polyposis coli - inducing mutations in APC gene in Poland

Autorzy:

Pawlak, A L
Plawski, A
Smoczkiewicz, P
Kwiatkowska, J
Meissner, W
Krokowicz, P
West, S P

Powiązania:

https://bibliotekanauki.pl/articles/2046600.pdf

Data publikacji:

1997

Wydawca:

Polska Akademia Nauk. Czytelnia Czasopism PAN

Tematy:

familial adenomatous polyposis
protein truncation test
Polish population
mutation
polymerase chain reaction
heteroduplex analysis

Opis:

Screening for molecular changes within the adenomatous polyposis coli (APC) gene, exons 11-14 and the 5’ half of exon 15, encompassing the mutation cluster region within exon 15, was performed in 30 patients with Familial Polyposis Coli (FAP). All patients were studied by heteroduplex analysis (HA) and single strand conformation polymorphism (SSCP) and molecular changes were found in 7 cases. Protein truncation test (PTT) has been performed in 17 cases in which mutations have not been found earlier, and shortening of protein product was noted in 2 cases. In three cases common deletion of 5 bp at codon 1309 and in one 5 bp deletion at codon 1061 were found. In other cases the molecular changes were demonstrated as heteroduplexes in exon 14 (1 patient), in segments E and F (one patient each) of exon 15, and in two cases the heteroduplexes were within the overlapping sequences of segments E/F and F/G of exon 15, respectively. In families where the molecular changes were found by HA, 7 persons at high risk for FAP were found and advised to undergo regular endoscopic examinations. In three persons at risk the transfer of mutation was excluded.

Źródło:

Journal of Applied Genetics; 1997, 38, 1; 77-85
1234-1983

Pojawia się w:

Journal of Applied Genetics

Dostawca treści:

Biblioteka Nauki

/5

Artykuł

Zmień widok

• na półce

Skocz do pozycji: 53.

Tytuł:

Construction of new GFP-tagged fusants for Trichoderma harzianum with enhanced biocontrol activity

Autorzy:

Kowsari, M.
Motallebi, M.
Zamani, R.M.

Powiązania:

https://bibliotekanauki.pl/articles/66878.pdf

Data publikacji:

2014

Wydawca:

Polska Akademia Nauk. Czytelnia Czasopism PAN

Tematy:

biomonitor
construction
Trichoderma harzianum
biological control
green fluorescent protein
real-time polymerase chain reaction

Opis:

Trichoderma is one of the most exploited biocontrol agents for the management of plant diseases. In biocontrol ecology, the critical factors are detection, and the monitoring and recovery of specific biocontrol agents either naturally present or deliberately released into the environment. Protoplast fusion is an appropriate tool for the improvement of biocontrol Trichoderma strains. Protoplast isolation from Trichoderma harzianum was achieved using 24 h culture age, 6.6 mg/ml Novazym L 1412 at 30°C which resulted the maximum protoplast yield of 5 × 108/ml. The self-fused protoplasts were regenerated and 12 fusants were selected based on their growth rate on 2% colloidal chitin medium. Next, a comparison was done for chitinase and antagonistic activity. Transcriptomic analysis based on quantitative real-time RT-PCR, demonstrated that T8-05 fusant expressed 1.5 fold of chit42 transcript as compared with the parental line. This fusant with 7.02±0.15U chitinase activity showed a higher growth inhibition rate (100%) than the parent strain, against Rhizoctonia solani. To obtain a genetically marked fusant that can be used as a biomonitor, the fusant was cotransformed with the gfp and amdS genes. The morphology and viability of selected cotransformant (FT8-7MK-05-2) was similar to the parent. Green fluorescing conidia were observed within the first 2 days of incubation in the soil, and this was followed by the formation of chlamydopores after 60 days. The colonisation of the gfp-tagged fusant was also monitored visually on R. solani sclerotia by scanning electron microscopy. Production of gfp-tagged fusant of Trichoderma spp. provides a potentially useful tool for monitoring hyphal growth patterns and the population of biocontrol agent isolates introduced into environmental systems.

Źródło:

Journal of Plant Protection Research; 2014, 54, 2
1427-4345

Pojawia się w:

Journal of Plant Protection Research

Dostawca treści:

Biblioteka Nauki

/5

Artykuł

Zmień widok

• na półce

Skocz do pozycji: 54.

Tytuł:

Detectability of tick-borne agents DNA in the blood of dogs, undergoing treatment for borreliosis

Autorzy:

Wodecka, B
Rymaszewska, A.
Sawczuk, M.
Skotarczak, B.

Powiązania:

https://bibliotekanauki.pl/articles/50622.pdf

Data publikacji:

2009

Wydawca:

Instytut Medycyny Wsi

Tematy:

dog
tick-borne agent
polymerase chain reaction
RFLP method
borreliosis
anaplasmosis
diagnostics
blood
treatment

Źródło:

Annals of Agricultural and Environmental Medicine; 2009, 16, 1; 9-14
1232-1966

Pojawia się w:

Annals of Agricultural and Environmental Medicine

Dostawca treści:

Biblioteka Nauki

/5

Artykuł

Zmień widok

• na półce

Skocz do pozycji: 55.

Tytuł:

Occurrence of virulence genes among Campylobacter jejuni and Campylobacter coli isolates from domestic animals and children

Autorzy:

Andrzejewska, M.
Klawe, J.J.
Szczepanska, B.
Spica, D.

Powiązania:

https://bibliotekanauki.pl/articles/30802.pdf

Data publikacji:

2011

Wydawca:

Polska Akademia Nauk. Czytelnia Czasopism PAN

Tematy:

occurrence
virulence gene
Campylobacter jejuni
Campylobacter coli
isolate
domestic animal
child
polymerase chain reaction

Opis:

The presence of the flaA, cadF, cdtB and iam genes of Campylobacter spp. was determined with the PCR method. The materials to investigate were 56 C. jejuni and 23 C. coli strains isolated from clinical samples (children and domestic animals). It was found that all of the Campylobacter spp. isolates from children with diarrhoea and domestic animals had cadF gene, responsible for adherence. The flaA gene was present in all Campylobacter spp. isolates derived from children and cats. Occurrence of flaA gene was confirmed in 100% of C. jejuni strains obtained from dogs. The high prevalence of the cdtB gene associated with toxin production was observed in this study (100%-Campylobacter spp. isolates obtained from dogs and cats, 97.9%-Campylobacter spp. isolates from children). The isolates showed a wide variation for the presence of iam gene. The lowest prevalence (23.5%) was detected in Campylobacter spp. obtained from dogs. The highest rates of iam detection (91.6%) were revealed in C. coli isolates from children.

Źródło:

Polish Journal of Veterinary Sciences; 2011, 14, 2
1505-1773

Pojawia się w:

Polish Journal of Veterinary Sciences

Dostawca treści:

Biblioteka Nauki

/5

Artykuł

Zmień widok

• na półce

Skocz do pozycji: 56.

Tytuł:

Problems, limitations, and challenges in species identifcation of Ascomycota members on the basis of ITS regions

Autorzy:

Baturo-Cieśniewska, Anna
Pusz, Wojciech
Patejuk, Katarzyna

Powiązania:

https://bibliotekanauki.pl/articles/11543330.pdf

Data publikacji:

2020

Wydawca:

Polskie Towarzystwo Botaniczne

Tematy:

Ascomycota
fungi
internal transcribed spacer
molecular identification
polymerase chain reaction
barcode
rDNA
phylogenetic analysis

Opis:

The internal transcribed spacer (ITS) region is regarded as a formal fungal primary barcode with a high probability of the correct identification for a broad group of fungi. ITS sequences have been widely used to determine many fungal species and analysis of rDNA ITS is still one of the most popular tools used in mycology. However, this region is not equally variable in all groups of fungi; therefore, identification may be problematic and result in ambiguous data, especially in some species-rich genera of Ascomycota. For these reasons, identification based on rDNA ITS is usually complemented by morphological observations and analysis of additional genes. Reliable species identification of Ascomycota members is essential in diagnosing plant diseases, verifying air quality and the effectiveness of agronomic practices, or analyzing relationships between microorganisms. Therefore, the present study aimed to verify, using specific examples, the extent to which ITS sequence analysis is useful in species identification of pathogens and saprobionts from Ascomycota and demonstrate problems related to such identification in practice. We analyzed 105 ITS sequences of isolates originating from air and plant material. Basic local alignment search tool (BLASTn) significantly contributed to the reliable species identification of nearly 80% of isolates such as Arthrinium arundinis, Beauveria bassiana, Boeremia exigua, Cladosporium cladosporioides, Epicoccum nigrum, Nigrospora oryzae, Sclerotinia sclerotiorum, or Sordaria fimicola and members of the genera Alternaria and Trichoderma. However, for most isolates, additional morphological observations, information regarding the isolate origin and, where possible, a PCR with species-specific primers were helpful and complementary. Using our practical approach, we determined that ITS-based species identification and comparative analysis with GenBank sequences significantly helps identifying Ascomycota members. However, in many cases, this should be regarded as suggestive of a taxon because the data usually require the use of additional tools to verify the results of such analysis.

Źródło:

Acta Mycologica; 2020, 55, 1; 5512
0001-625X
2353-074X

Pojawia się w:

Acta Mycologica

Dostawca treści:

Biblioteka Nauki

/5

Artykuł

Zmień widok

• na półce

Skocz do pozycji: 57.

Tytuł:

Mg2+ ions do not induce expansion of the melted DNA region in the open complex formed by Escherichia coli RNA polymerase at a cognate synthetic Pa promoter. A quantitative KMnO4 footprinting study

Autorzy:

Łoziński, Tomasz
Wierzchowski, Kazimierz

Powiązania:

https://bibliotekanauki.pl/articles/1044146.pdf

Data publikacji:

2001

Wydawca:

Polskie Towarzystwo Biochemiczne

Tematy:

permanganate footprinting
open promoter complex
thymine oxidation
effect of magnesium ions
RNA polymerase

Opis:

Footprinting studies of prokaryotic open transcription complexes (RPO), based on oxidation of pyrimidine residues by KMnO4 and/or OsO4 at a single oxidant dose, have suggested that the extent of DNA melting in the transcription bubble region increases in the presence of Mg2+. In this work, quantitative KMnO4 footprinting in function of the oxidant dose of RPO, using Escherichia coli RNA polymerase (Eσ70 ) at a fully functional synthetic promoter Pa having -35 and -10 consensus hexamers, has been used to determine individual rate constants of oxidation of T residues in this region at 37°C in the absence of Mg2+ and in the presence of 10 mM MgCl2, and to evaluate therefrom the effect of Mg2+ on the extent of DNA melting. Population distributions of end-labeled DNA fragments corresponding to oxidized Ts were quantified and analyzed according to the single-hit kinetic model. Pseudo-first order reactivity rate constants, ki, thus obtained demonstrated that Mg2+ ions bound to RPO merely enhanced the reactivity of all 11 oxidizable thymines between the +3 and -11 promoter sites by a position-dependent factor: 3-4 for those located close to the transcription start point +1 in either DNA strand, and about 1.6 for those located more distantly therefrom. On the basis of these observations, we conclude that Mg2+ ions bound to RPO at Pa do not influence the length of the melted DNA region and propose that the higher reactivity of thymines results mainly from lower local repulsive electrostatic barriers to MnO4- diffusion around carboxylate binding sites in the catalytic center of RPO and promoter DNA phosphates.

Źródło:

Acta Biochimica Polonica; 2001, 48, 2; 495-510
0001-527X

Pojawia się w:

Acta Biochimica Polonica

Dostawca treści:

Biblioteka Nauki

/5

Artykuł

Zmień widok

• na półce

Skocz do pozycji: 58.

Tytuł:

Diversity of Pseudomonas species associated with soft rot of plants in Poland

Autorzy:

Zolobowska, L
Pospieszny, H.

Powiązania:

https://bibliotekanauki.pl/articles/65211.pdf

Data publikacji:

2001

Wydawca:

Polska Akademia Nauk. Czytelnia Czasopism PAN

Tematy:

Pseudomonas fluorescens
Polska
Pseudomonas
polymerase chain reaction
occurrence
Pseudomonas putida
heterogeneity
identification
plant

Opis:

A total of 94 pectolytic and 60 nonpectolytic Pseudomonas isolates were obtained from 250 samples of rotted vegetable specimens representing various economically important vegetables. The isolates were identified on the basis of standard biochemical tests. Pseudomonas fluorescens biovar V and II and Pseudomonas putida were the most abundant species among pectolytic isolates and Pseudomonas fluorescens biovar I among nonpectolytic ones. Only 3 Pseudomonas viridiflava isolates were identified and all of them were obtained from potato. Isolates of pectolytic phenotype were scattered among nonpectolytic ones irrespective of their taxonomical status. Isolates identified biochemically, as Pseudomonas marginalis were also present in nonpectolytic group. PCR method is unsuitable for identification and differentiation of bacteria belonging to pectolytic fluorescens Pseudomonas group due to great diversity of species. However, the results of PCR amplification of the genes encoding pectate lyase suggest that genes responsible for production of this enzyme may also be present in isolates of nonpectolytic phenotype.
Z 250 prób, z różnych gatunków roślin warzywnych z objawami mokrej zgnilizny wyodrębniono 94 izolaty pektynolitycznych i 60 izolatów nie pektynolitycznych bakterii z rodzaju Pseudomonas. Identyfikację i różnicowanie izolatów przeprowadzono przy zastosowaniu standardowych testów fizjologiczno-biochemicznych. Spośród izolatów z grupy pektolitycznych Pseudomonas najliczniej występował gatunek P. fluorescens, który był reprezentowany przez 4 biowary (oprócz czwartego), przy czym dominowały biowary II i V. Mniej licznie występował gatunek P. putida, a P. viridiflava był reprezentowany jedynie przez 3 izolaty, pochodzące z ziemniaka. Podobna była struktura populacji izolatów z grupy niepektolitycznych Pseudomonas. Metoda PCR okazała się być mało przydatna do wykrywania i różnicowania izolatów z grupy pektolitycznych Pseudomonas ze względu na duże ich zróżnicowanie. Amplifikacja DNA metodą PCR wykazała, że geny kodujące enzym liazy pektynowej występują także w izolatach niepektolitycznych rodzaju Pseudomonas.

Źródło:

Journal of Plant Protection Research; 2001, 41, 1
1427-4345

Pojawia się w:

Journal of Plant Protection Research

Dostawca treści:

Biblioteka Nauki

/5

Artykuł

Zmień widok

• na półce

Skocz do pozycji: 59.

Tytuł:

Isolation of the DNA fragment reflecting the open reading frame II of the I-18 C gene of Chironomus tentans by the polymerase chain reaction. II. The technology for the preparation of the template

Autorzy:

Borowicz, B P

Powiązania:

https://bibliotekanauki.pl/articles/65476.pdf

Data publikacji:

1997

Wydawca:

Polska Akademia Nauk. Czytelnia Czasopism PAN

Tematy:

gene expression
I-18 C gene
Chironomus tentans
polymerase chain reaction
DNA fragment

Opis:

The technology for the preparation of the template in the research aimed to isolate the DNA fragment reflecting the Open Reading Frame II of the I-18 C gene is presented i.e. the linearisation of the template by Bam H I digestion, the purification of the template and the estimation of the template concentration.
Przedstawiono technologię przygotowywania matrycy DNA w badaniach zmierzających do izolacji Otwartej Ramy Odczytu II genu I-18 C Chironomus tentans. Technologia ta obejmowała linearyzację DNA matrycy poprzez trawienie Bam H I oraz oczyszczenie matrycy i oznaczenie stężenia matrycowego DNA.

Źródło:

Journal of Plant Protection Research; 1997, 37, 1-2
1427-4345

Pojawia się w:

Journal of Plant Protection Research

Dostawca treści:

Biblioteka Nauki

/5

Artykuł

Zmień widok

• na półce

Skocz do pozycji: 60.

Tytuł:

Cloning of the DNA fragments reflecting the open reading frame I and II of the I-18 C gene of Chironomus tentans. II. Preparation of the intermediate vector - bluescript plasmid for ligation with the inserts

Autorzy:

Borowicz, B P

Powiązania:

https://bibliotekanauki.pl/articles/66467.pdf

Data publikacji:

1999

Wydawca:

Polska Akademia Nauk. Czytelnia Czasopism PAN

Tematy:

cloning
I-18 C gene
plasmid
Chironomus tentans
polymerase chain reaction
DNA fragment

Opis:

The technology used for the cloning of the open reading frame (ORF) I and II reflecting DNA fragments of the I-18 C gene in regard to the preparation of the intermediate vector i.e. the bluescript for the ligation with the ORF I and II reflecting DNA fragments, is presented. The main steps include the Eco RV digestion of the bluescript plasmid, the phenol / methylene chloride extraction of the plasmid, dephosphorylation of the bluescript plasmid and finally its extraction with the phenol and ethanol precipitation.
Przedstawiono technologię, którą zastosowano do klonowania fragmentów DNA odzwierciedlających translacyjną otwartą ramę odczytu I i II genu I-18 C Chironomus tentans w zakresie: przygotowania wektora pośredniego - plazmidu blueskrypt do ligacji z wymienionymi sekwencjami wstawek. Główne etapy obejmują: trawienie plazmidu blueskrypt enzymem Eco RV, ekstrakcję plazmidu fenolem/ chlorkiem metylenu, defosforylację plazmidu blueskrypt, ostatecznie jego oczyszczenie poprzez ekstrakcję fenolową i precypitację etanolem.

Źródło:

Journal of Plant Protection Research; 1999, 39, 1
1427-4345

Pojawia się w:

Journal of Plant Protection Research

Dostawca treści:

Biblioteka Nauki

/5

Artykuł

Zmień widok

• na półce

Skocz do pozycji: 61.

Tytuł:

Diagnosis of Pneumocystis carinii infection by a serum polymerase chain reaction

Autorzy:

Golab, E.
Sobolewska, A.
Dzbenski, T.
Bitkowska, E.

Powiązania:

https://bibliotekanauki.pl/articles/836353.pdf

Data publikacji:

1998

Wydawca:

Polskie Towarzystwo Parazytologiczne

Tematy:

Pneumocystis carinii
infection
serum
diagnosis
laboratory diagnosis
pneumonia
polymerase chain reaction
rRNA gene
T cell

Źródło:

Annals of Parasitology; 1998, 44, 3
0043-5163

Pojawia się w:

Annals of Parasitology

Dostawca treści:

Biblioteka Nauki

/5

Artykuł

Zmień widok

• na półce

Skocz do pozycji: 62.

Tytuł:

A new role for thioredoxin z in modulating the redox regulation activity of other thioredoxin in chloroplasts

Autorzy:

Bohrer, A.
Issakidis-Bourguet, E.
Vanacker, H.

Powiązania:

https://bibliotekanauki.pl/articles/80975.pdf

Data publikacji:

2013

Wydawca:

Polska Akademia Nauk. Czytelnia Czasopism PAN

Tematy:

conference
plant adaptation
environmental stress condition
thioredoxin
Arabidopsis
antioxidative enzyme
RNA polymerase complex
chloroplast
ferredoxin

Źródło:

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2013, 94, 2
0860-7796

Pojawia się w:

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

Dostawca treści:

Biblioteka Nauki

/5

Artykuł

Zmień widok

• na półce

Skocz do pozycji: 63.

Tytuł:

Isolation of the DNA fragment reflecting the open reading frame II of the I-18 C gene of Chironomus tentans by the polymerase chain reaction. V. Different mechanisms of regulation regarding the open reading frames

Autorzy:

Borowicz, B P

Powiązania:

https://bibliotekanauki.pl/articles/64980.pdf

Data publikacji:

1997

Wydawca:

Polska Akademia Nauk. Czytelnia Czasopism PAN

Tematy:

regulation mechanism
gene expression
I-18 C gene
Chironomus tentans
polymerase chain reaction
DNA fragment

Opis:

Different mechanisms of regulation regarding the Open Reading Frames (ORFs) have been discussed to have a broader perspective that is necessary to evaluate the role of the ORFs of the I-18 C gene. This consideration includes the ORF II of the I-18 C gene which is the object of the presented research.
Omówiono różnorodne mechanizmy regulacji dotyczące Otwartych Ram Odczytu, w celu wytworzenia szerszego spojrzenia na to zagadnienie, które jest potrzebne do oceny roli poszczególnych Otwartych Ram Odczytu (ORF) genu I-18 C. Omówienie to dotyczy również ORF II genu I-18 C, co było przedmiotem zaprezentowanych badań.

Źródło:

Journal of Plant Protection Research; 1997, 37, 1-2
1427-4345

Pojawia się w:

Journal of Plant Protection Research

Dostawca treści:

Biblioteka Nauki

/5

Artykuł

Zmień widok

• na półce

Skocz do pozycji: 64.

Tytuł:

The use reverse transcription and polymerase chain reaction [RT-PCR] for the detection of hop latent viroid [HLVd]

Autorzy:

Cajza, M
Wypijewski, K.
Pospieszny, H.

Powiązania:

https://bibliotekanauki.pl/articles/65773.pdf

Data publikacji:

1997

Wydawca:

Polska Akademia Nauk. Czytelnia Czasopism PAN

Tematy:

hop latent viroid
reverse transcription
viroid
hop
plant pathogen
polymerase chain reaction
detection
DNA amplification

Opis:

The simple method of nucleic acids extraction, based on guanidine thiocyanate extraction buffer, was sufficient for obtaining a good templates for RT-PCR. The RT-PCR reactions were performer from the start to the end (both the reverse transcription and the HLVd-cDNA amplification) in the same reaction mixture and in the very small volume of reaction (10 μI). Both pairs of primers designed by authors were good for reverse transcription and later for amplification of the HLVd-cDNA. The presence of gelatin as a stabilizer of DNA polymerase was indispensable for successful performance of RT-PCR.
Wiroidy, najmniejsze obecnie znane patogeny roślin, zbudowane z pojedynczej nici kolistego RNA, nie zawierające w swej budowie i nie kodujące żadnych białek, są groźnymi patogenami roślin wyższych, rozpowszechnionymi we wszystkich rejonach świata, szczególnie w uprawach roślin rozmnażanych wegetatywnie. Wiroid latentny chmielu (ang. hop latent viroid - HLVd) został wykryty dopiero w 1988 roku w chmielu. Do tej pory odnotowano go w rejonach uprawy chmielu na całym świecie. Chmiel, jedyny znany żywiciel tego patogena, ulega tylko infekcjom utajonym (latentnym). HLVd powoduje zarówno spadek masy plonu szyszek jak i zawartości alfa-kwasów w szyszkach. Bezobjawowe infekcje oraz brak białek strukturalnych i innych produktów translacji powodują, że obecnie patogena tego można wykrywać tylko przy pomocy elektroforezy (metoda bardzo zawodna, wymagająca wysokiego stężenia RNA patogena w tkance), sond hybrydyzacyjnych i rozwijanej w ostatnich latach metody RT-PCR. Podczas opracowywania RT-PCR do wykrywania HLVd w ekstraktach kwasów nukleinowych wykorzystywano dwie pary primerów specyficznych dla sekwencji nukleotydowej HLVd, charakteryzujących się różnymi temperaturami topnienia produktu. Wiroida wykrywano w ekstraktach kwasów nukleinowych z blaszek liściowych i z ogonków liściowych chmielu. Uproszczona metoda guanidynowa do izolacji totalnych kwasów nukleinowych okazała się wystarczająca do przeprowadzenia reakcji RT-PCR. Opracowana metoda charakteryzowała się bardzo dużą czułością, specyficznością (produkty amplifikacji cDNA-HLVd uzyskiwano tylko z próbek materiału roślinnego pochodzącego z roślin zawierających tego wiroida) i szybkością wykonania (dwa dni łącznie z ekstrakcją kwasów nukleinowych). Ponadto w zastosowanej metodzie opracowano warunki do przeprowadzenia zarówno odwrotnej transkrypcji i następnie amplifikacji powstałego cDNA wiroida w jednej probówce, w tej samej mieszaninie reakcyjnej . Oprócz tego wszystkie reakcje RT-PCR prowadzono w bardzo małej objętości równej 10 μl (2 μl zawiesiny kwasów nukleinowych i 8 μl mieszaniny reakcji RT-PCR). Maksymalne uproszczenie metody, wielokrotne obniżenie kosztów reakcji oraz charakterystyczna dla RT-PCR czułość, specyficzność i szybkość przeprowadzenia testu sprawiają, że może być ona wykorzystywana do rutynowego wykrywania nie tylko HLVd ale również innych wiroidów, wirusoidów i wirusów RNA.

Źródło:

Journal of Plant Protection Research; 1997, 37, 1-2
1427-4345

Pojawia się w:

Journal of Plant Protection Research

Dostawca treści:

Biblioteka Nauki

/5

Artykuł

Zmień widok

• na półce

Skocz do pozycji: 65.

Tytuł:

Some evidence for skewed mating type distribution in Iranian populations of Rhynchosporium commune, the cause of barley scald disease

Autorzy:

Arzanlou, M.
Karimi, K.
Mirabi, F.

Powiązania:

https://bibliotekanauki.pl/articles/66749.pdf

Data publikacji:

2016

Wydawca:

Polska Akademia Nauk. Czytelnia Czasopism PAN

Tematy:

mating type
distribution
Rhynchosporium commune
barley
scald disease
foliar disease
scald
multiplex polymerase chain reaction

Opis:

Rhynchosporium commune (formerly known as Rhynchosporium secalis), the causal agent of scald disease on barley, is known to spread asexually by splash dispersed conidia. However, there are multiple lines of evidence for the possibility of a clandestine sexual cycle occurrence in this species including extensive genotypic diversity, equal distribution of mating type alleles across the world and expression of mating type genes. In the current study, the potential for the occurrence of a sexual cycle amongst the Iranian population of R. commune was assessed by analyzing distribution and frequency of the mating type alleles at both micro and macrospatial scales. A total of 95 single-conidial R. commune isolates were obtained from different barley fields in Kurdistan province. Previously designed primers were applied in a multiplex PCR assay to study distribution and frequency of the mating type alleles within and between populations. Totally, 67 isolates were determined as MAT1-1 and the remaining 28 isolates as MAT1-2 throughout the sampling counties. The results obtained at a macro-spatial scale revealed that unlike Kamyaran county (both MAT1-1 and MAT1-2 at an equal ratio), an unequal distribution of mating type genes was dominant among R. commune isolates in both Mariwan and Dehgolan counties. Our findings support a predominantly asexual reproduction for Mariwan and Dehgolan counties and the possibility of sexual stage occurrence in Kamyarna county.

Źródło:

Journal of Plant Protection Research; 2016, 56, 3
1427-4345

Pojawia się w:

Journal of Plant Protection Research

Dostawca treści:

Biblioteka Nauki

/5

Artykuł

Zmień widok

• na półce

Skocz do pozycji: 66.

Tytuł:

Isolation of the DNA fragment reflecting the open reading frame II of the I-18 C gene of Chironomus tentans by the polymerase chain reaction. III. The DNA amplification technology

Autorzy:

Borowicz, B P

Powiązania:

https://bibliotekanauki.pl/articles/66760.pdf

Data publikacji:

1997

Wydawca:

Polska Akademia Nauk. Czytelnia Czasopism PAN

Tematy:

gene expression
I-18 C gene
Chironomus tentans
polymerase chain reaction
DNA fragment
DNA amplification

Opis:

The technology used to amplify and isolate the DNA fragment reflecting the Open Reading Frame II of the I-18 C gene of Chironomus tentans is described i.e. the Polymerase Chain Reactioin and the agarose gel electrophoresis of this reaction product, as well as the blunting reaction of the product and its isolation.
Opisano technologię zastosowaną do powielenia i izolacji fragmentu DNA odpowiadającego Otwartej Ramie Odczytu II genu I-18 C Chironomus tentans, tj. Reakcję Łańcuchową Polimerazy (PCR) oraz elektroforezę w żelu agarozowym produktu tej reakcji jak również reakcję tzw. Stępiania końców wyżej wymienionego produktu i ostatecznie jego izolację .

Źródło:

Journal of Plant Protection Research; 1997, 37, 1-2
1427-4345

Pojawia się w:

Journal of Plant Protection Research

Dostawca treści:

Biblioteka Nauki

/5

Artykuł

Zmień widok

• na półce

Skocz do pozycji: 67.

Tytuł:

HybProbes-based real-time PCR assay for rapid detection of equine herpesvirus type 2 DNA

Autorzy:

Osinska, E.
Golke, A.
Slonska, A.
Cymerys, J.
Banbura, M.W.
Dzieciatkowski, T.

Powiązania:

https://bibliotekanauki.pl/articles/30252.pdf

Data publikacji:

2012

Wydawca:

Polska Akademia Nauk. Czytelnia Czasopism PAN

Tematy:

rapid detection
equine herpesvirus
real-time polymerase chain reaction
horse
herpesvirus
Gammaherpesvirinae
Rhadinovirus
veterinary virology

Opis:

Equid herpesvirus type 2 (EHV-2) together with equid herpesvirus type 5 are members of Gammaherpesvirinae subfamily, genus Rhadinovirus. EHV-2 is one of major agents causing diseases of horses common worldwide. A possible role of EHV-2 in reactivating latent equid herpesvirus type-1 has been suggested, because reactivation of latent EHV-1 was always accompanied by EHV-2 replication. Variety techniques, including cell culture, PCR and its modifications, have been used to diagnose EHV-2 infections. The aim of this study was to develop, optimize and determine specificity of real-time PCR (qPCR) for EHV-2 DNA detection using HybProbesR chemistry and to evaluate clinical samples with this method. The analytical sensitivity of assay was tested using serial dilutions of viral DNA in range between 70 and 7x105 copies/ml. The limit of detection (LOD) was calculated using probit analysis and was determined as 56 copies/ml. In further studies 20 different clinical samples were tested for the presence of EHV-2. Described in-house qPCR method detected viral DNA in 5 of 20 specimens used. The results of this work show that developed HybProbes-based real-time PCR assay is very reliable and valuable for detection and quantification of equid herpesvirus type 2 DNA in different clinical samples. The high level of sensitivity, accuracy and rapidity provided by the LightCycler 2.0 instrument are favorable for the use of this system in the detection of EHV-2 DNA in veterinary virology.

Źródło:

Polish Journal of Veterinary Sciences; 2012, 15, 3
1505-1773

Pojawia się w:

Polish Journal of Veterinary Sciences

Dostawca treści:

Biblioteka Nauki

/5

Artykuł

Zmień widok

• na półce

Skocz do pozycji: 68.

Tytuł:

Differentiation of Listeria monocytogenes on the basis of hemolytic and phosphatidylinositol specific phospholipase C activity and by PCR method

Autorzy:

Kotlowski, R.
Kaczmarek, M.
Kur, J.
Rudnicka, W.

Powiązania:

https://bibliotekanauki.pl/articles/1372565.pdf

Data publikacji:

1996

Wydawca:

Instytut Rozrodu Zwierząt i Badań Żywności Polskiej Akademii Nauk w Olsztynie

Tematy:

phosphatidylinositol-specific phospholipase C
phospholipase C
Listeria monocytogenes
bacteria
food preservation
polymerase chain reaction
hemolysis

Opis:

The possibility of differentiation of L.monocytogenes from other Listeria species on the basis of hemolytic activity, the production of phosphatidylinositol-specific phospholipase C (PI-PLC) and the polymerase chain reaction (PCR) for the amplification of a DNA fragment of listeriolysine O (hly A) gene was compared. The screening of Listeria colonies for PI-PLC activity allowed to distinguish the pathogenic for humans L.monocytogenes bacteria from the majority of non-pathogenic Listeria spp. The amplification of DNA from Listeria lysates with two primers selected in area of the hly A gene made possible the differentiation of L.monocytogenes from other Listeria species, including hemolytic L.ivanovii and L.seeligeri bacteria as well as hemolytic or PI-PLC positive L.innocua strains.
Żywność zanieczyszczona Listeria monocytogenes byía notowana jako źródło zakażeń pokarmowych o charakterze epidemicznym w krajach w Europie i Ameryce Północnej. W związku z tym koniecznością się stalo opracowanie szybkich i czuîych testów wykrywania i identyfikacji pałeczek L. monocytogenes. W pracy porównano przydatność trzech testów do odróżniania chorobotwórczych dla człowieka bakterii L. monocytogenes od innych niepatogennych gatunków Listeria. Uzyskane wyniki wskazują, na niewielką przydatność oceny aktywności hemolitycznej dla różnicowania szczepów L. monocytogenes od niechorobotwórczych dla ludzi szczepów L. ivanovii, L. seeligeri oraz L. innocua. Różnicowanie takich szczepów w oparciu o aktywność fosfolipazy C fosfatydyloinozytolu wykazuje wyższą specyficzność. Cechę taką wykazywały wszystkie badane szczepy L. monocytogenes i tylko jeden szczep L. innocua spośród 9 niepatogennych szczepów Listeria. Amplifikacja fragmentu DNA w obrębie genu dla listeriolizyny O (hly A) L. monocytogenes, pozwoliła odróżnić wszystkie (18) szczepy L. monocytogenes od niepatogennych dla człowieka bakterii L. ivanovii, L. innocua, L. seeligeri, L. welshimeri oraz L. grayi. Trawienie produktu amplifikacji DNA enzymem Taq I, pozwoliło na wyodrębnienie dwóch typów w obrębie grupy badanych szczepów L. monocytogenes.

Źródło:

Polish Journal of Food and Nutrition Sciences; 1996, 05, 3; 99-109
1230-0322
2083-6007

Pojawia się w:

Polish Journal of Food and Nutrition Sciences

Dostawca treści:

Biblioteka Nauki

/5

Artykuł

Zmień widok

• na półce

Skocz do pozycji: 69.

Tytuł:

Adaptor-mediated amplification of minute amounts of severely fragmented ancient nucleic acids

Autorzy:

Pusch, C M
Blin, N.
Broghammer, M.
Nicholson, G.J.
Scholz, M.

Powiązania:

https://bibliotekanauki.pl/articles/2042022.pdf

Data publikacji:

2001

Wydawca:

Polska Akademia Nauk. Czytelnia Czasopism PAN

Tematy:

mitochondrial DNA
genetics
nucleic acid
ancient DNA
polymerase chain reaction
DNA
cDNA
sex typing

Źródło:

Journal of Applied Genetics; 2000, 41, 4; 303-315
1234-1983

Pojawia się w:

Journal of Applied Genetics

Dostawca treści:

Biblioteka Nauki

/5

Artykuł

Zmień widok

• na półce

Skocz do pozycji: 70.

Tytuł:

The use amelogenin gene polymorphism in PCR embryo sexing in bovine IVF embryos

Autorzy:

Lechniak, D
Cumming, J R

Powiązania:

https://bibliotekanauki.pl/articles/2046605.pdf

Data publikacji:

1997

Wydawca:

Polska Akademia Nauk. Czytelnia Czasopism PAN

Tematy:

polymorphism
sex chromosome
Y chromosome
bovine embryo
sex
amelogenin gene
polymerase chain reaction
embryo

Opis:

The present study describes a rapid, simple method of bovine IVF embryo sexing by use of PCR technique. A pair of primers corresponding to the bovine amelogenin sequence has been used. The Rapid Cycler (Idaho Technology, USA) used in the current experiment enabled the PCR programme consisting of 55 cycles to be completed in less than 40 minutes. Therefore the total sexing procedure could be performed in less than 90 minutes. The described method succeded in case of 85% analysed embryos.

Źródło:

Journal of Applied Genetics; 1997, 38, 1; 45-49
1234-1983

Pojawia się w:

Journal of Applied Genetics

Dostawca treści:

Biblioteka Nauki

/5

Artykuł

Zmień widok

• na półce

Skocz do pozycji: 71.

Tytuł:

Wild game as a reservoir of Anaplasma phagocytophilum in North-Western Poland

Autorzy:

Adamska, M.
Skotarczak, B.

Powiązania:

https://bibliotekanauki.pl/articles/2143862.pdf

Data publikacji:

2007

Wydawca:

Polskie Towarzystwo Parazytologiczne

Tematy:

parasite
Polska
reservoir
roe deer
bacteria
Anaplasma phagocytophilum
polymerase chain reaction
intracellular parasite
tick

Opis:

Background. The aim of the study was to determine the role of game animals as reservoirs of Anaplasma phagocytophilum, a bacteria species transmitted by Ixodes ricinus ticks, from north−western Poland (Zachodniopomorskie vovoidship). The area under question is endemic for A. phagocytophilum. Material and methods. Blood and spleen samples were taken from 72 roe deer between April and December 2003. Animals culled during winter did not harbour ticks, on the other hand 155 individuals of Ixodes ricinus were collected from 35 of 43 animals taken during spring. We tested all samples for A. phagocytophilum by PCR amplification of the msp2 gene. An individual was considered infected if pathogens were detected in at least one isolate (blood or a tissue sample). Results. DNA from A. phagocytophilum was not found in isolates from ticks collected from the animals. The general level of infection for the roe deer was 31.94% (23/72). DNA of A. phagocytophilum was most commonly detected in blood samples; only in three cases was anaplasma DNA detected in spleen and not in blood. Ruminants seem to be the most competent reservoir for A. phagocytophilum in north−western Poland.

Źródło:

Wiadomości Parazytologiczne; 2007, 53, 2; 103-107
0043-5163

Pojawia się w:

Wiadomości Parazytologiczne

Dostawca treści:

Biblioteka Nauki

/5

Artykuł

Zmień widok

• na półce

Skocz do pozycji: 72.

Tytuł:

Dedifferentiation of Arabidopsis thaliana cells is accompanied by large decrease of RNA polymerase II transcription

Autorzy:

Delenko, K.
Niedojadlo, J.
Labedzka, A.
Bednarska-Kozakiewicz, E.

Powiązania:

https://bibliotekanauki.pl/articles/80652.pdf

Data publikacji:

2013

Wydawca:

Polska Akademia Nauk. Czytelnia Czasopism PAN

Tematy:

conference
Arabidopsis thaliana
dedifferentiation
RNA polymerase II
cell wall
plant
mesophyll cell
heterochromatin
monoclonal antibody
phosphoserine

Źródło:

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2013, 94, 3
0860-7796

Pojawia się w:

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

Dostawca treści:

Biblioteka Nauki

/5

Artykuł

Zmień widok

• na półce

Skocz do pozycji: 73.

Tytuł:

Aktyna i miozyny w jądrze komórkowym
Actins and myosins in the nucleus

Autorzy:

Nowak, Jolanta
Rędowicz, Maria

Powiązania:

https://bibliotekanauki.pl/articles/1033936.pdf

Data publikacji:

2018

Wydawca:

Polskie Towarzystwo Przyrodników im. Kopernika

Tematy:

actin
gene expression
myosin
nucleus
nucleolus
polymerase
transcription
aktyna
ekspresja genów
miozyna
jądro
jąderko
polimeraza
transkrypcja

Opis:

Aktyna i miozyna to białka kojarzone przede wszystkim z ich kluczową rolą w generacji skurczu mięśni. Natomiast poza izoformami charakterystycznymi dla mięśni są również izoformy aktyny i miozyny, które występują we wszystkich typach komórek i tkanek (patrz artykuł Suszek i współaut. w tym zeszycie KOSMOSU). Badania prowadzone w ostatnich dwóch dekadach wykazały niezbicie, że zarówno aktyna (i szereg białek wiążących aktynę) oraz liczne miozyny (przedstawiciele rodzin I, II, V, VI, XVI i XVIII) lokalizują się w jądrze komórkowym gdzie są zaangażowane w procesy transkrypcji i naprawy DNA, transport w nukleoplazmie oraz import i eksport jądrowy, a także w utrzymywanie architektury jądra. Niniejszy artykuł opisuje dotychczasowy stan wiedzy o roli układu akto-miozynowego w jądrze komórkowym.
Actin and myosins are the proteins mainly known from their key roles in muscle contraction. However, besides typical muscle isoforms there are actins and myosins that are present in all cell and tissue types. Studies performed within the last two decades have irrefutably shown that both the cytoplasmic actin isoforms (along with numerous actin-binding proteins) as well as many myosins (representing class I, II, V, VI, XVI and XVIII) are present within the nucleus. They play important roles in nuclear processes as they are involved in transcription and DNA repair, intranuclear transport as well as nuclear import and export, and in maintenance of nuclear architecture. This article describes the current knowledge on the acto-myosin system in this biggest cellular compartment.

Źródło:

Kosmos; 2018, 67, 1; 75-93
0023-4249

Pojawia się w:

Kosmos

Dostawca treści:

Biblioteka Nauki

/5

Artykuł

Zmień widok

• na półce

Skocz do pozycji: 74.

Tytuł:

The quantitative PCR technique resolves ambiguities concerning a small rearrangement of human chromosome 6q12-13

Autorzy:

Nowacka, J
Helszer, Z.
Walter, Z.
Plucienniczak, A.
Kaluzewski, B.

Powiązania:

https://bibliotekanauki.pl/articles/2048327.pdf

Data publikacji:

2001

Wydawca:

Polska Akademia Nauk. Czytelnia Czasopism PAN

Tematy:

duplication
chromosome
human chromosome
man
rearrangement
cytogenetic diagnosis
karyotype analysis
chromosome aberration
polymerase chain reaction
inversion

Źródło:

Journal of Applied Genetics; 2001, 42, 4; 541-545
1234-1983

Pojawia się w:

Journal of Applied Genetics

Dostawca treści:

Biblioteka Nauki

/5

Artykuł

Zmień widok

• na półce

Skocz do pozycji: 75.

Tytuł:

Association of arylamine N-acetyltransferase [NAT1 and NAT2] genotypes with urinary bladder cancer risk

Autorzy:

Jaskula-Sztul, R
Sokolowski, W.
Gajecka, M.
Szyfter, K.

Powiązania:

https://bibliotekanauki.pl/articles/2041980.pdf

Data publikacji:

2001

Wydawca:

Polska Akademia Nauk. Czytelnia Czasopism PAN

Tematy:

tobacco smoking
human genetics
genotype
urinary bladder
polymerase chain reaction
cancer
acetylator genotype
arylamine N-acetyltransferase

Źródło:

Journal of Applied Genetics; 2001, 42, 2; 223-231
1234-1983

Pojawia się w:

Journal of Applied Genetics

Dostawca treści:

Biblioteka Nauki

/5

Artykuł

Zmień widok

• na półce

Skocz do pozycji: 76.

Tytuł:

Identification and characterization of Pseudomonas syringae pv. syringae strains from various plants and geographical regions

Autorzy:

Khezri, M.
Mohammadi, M.

Powiązania:

https://bibliotekanauki.pl/articles/65669.pdf

Data publikacji:

2018

Wydawca:

Polska Akademia Nauk. Czytelnia Czasopism PAN

Tematy:

identification
Pseudomonas syringae pv.syringae
plant
phenotype
polymerase chain reaction
protein profile
plasmid
syringomycin
geographic region

Opis:

Pseudomonas syringae pv. syringae (Pss) constitutes a diverse group of bacterial strains that cause canker of stone fruits, blight of cereals and red streak of sugarcane. The purpose of this study was to determine how diverse Iranian strains of Pss are when they come from different hosts. We compared a total of 32 Pss strains isolated from stone fruits, barley, wheat and sugarcane from different geographical regions of Iran based on their phenotypic and molecular properties. Strains showed some variation regarding carbon and nitrogen utilization. Pss strains were similar in their protein banding patterns. Additional bands were found in sugarcane strains. Most strains showed one indigenous plasmid DNA and a few had two and some none. The genes of syrB and syrD encoding syringomycin synthesis and secretion, respectively, were amplified using specific primers in polymerase chain reaction. Syringomycin, producing strains amplified two DNA fragments of 752 and 446 bp representing syrB and syrD genes, respectively. Primer specificity was shown for Pss using various genera. Based on the results of this study, it is suggested that Pss strains from different hosts and geographical regions show diversity in phenotypic and molecular characters. It is thought that phenotypic variation is due to adaptation to specific hosts and niches for survival and pathogenicity.

Źródło:

Journal of Plant Protection Research; 2018, 58, 4
1427-4345

Pojawia się w:

Journal of Plant Protection Research

Dostawca treści:

Biblioteka Nauki

/5

Artykuł

Zmień widok

• na półce

Skocz do pozycji: 77.

Tytuł:

Isolation of the DNA fragment reflecting the open reading frame II of the I-18 C gene of Chironomus tentans by the polymerase chain reaction. I. The technology for the preparation of the primers

Autorzy:

Borowicz, B P

Powiązania:

https://bibliotekanauki.pl/articles/65836.pdf

Data publikacji:

1997

Wydawca:

Polska Akademia Nauk. Czytelnia Czasopism PAN

Tematy:

phosphorylation
plant protection
I-18 C gene
purification
Chironomus tentans
polymerase chain reaction
DNA fragment
oligonucleotide

Opis:

The above mentioned technology in a case of the DNA fragment reflecting the Open Reading Frame II of the I-18 C gene is described i.e. the primers design, purification and phosphorylation of the oligonucleotide primers.
Dla określonego w tytule pracy, celu badań przedstawiono technologię przygotowywania starterów, w przypadku DNA odpowiadającego Otwartej Ramie Odczytu II genu I-18 C Chironomus tentans. Technologia ta obejmowała zaprojektowanie starterów dla PCR oraz oczyszczenie i fosforylację zsyntetyzowanych oligonukleotydowych starterów.

Źródło:

Journal of Plant Protection Research; 1997, 37, 1-2
1427-4345

Pojawia się w:

Journal of Plant Protection Research

Dostawca treści:

Biblioteka Nauki

/5

Artykuł

Zmień widok

• na półce

Skocz do pozycji: 78.

Tytuł:

Identification of Clavibacter michiganensis subsp. michiganensis by the polymerase chain reaction [PCR]

Autorzy:

Cajza, M
Rezler, A.
Pospieszny, H.

Powiązania:

https://bibliotekanauki.pl/articles/65945.pdf

Data publikacji:

1997

Wydawca:

Polska Akademia Nauk. Czytelnia Czasopism PAN

Tematy:

tomato seed
pathogen
Clavibacter michiganensis
plant protection
bacteria
polymerase chain reaction
detection
DNA amplification
seed
identification

Opis:

The PCR offers the possibility of specific and very sensitive detection and/or identification of Cl. m. subsp. michiganensis in seeds. The described PCR method for identification of this pathogen is very fast (one day) and economical, because of the very small volume (10 μI) of the PCR reaction mixture. The method based on amplification of the bacterial plasmid DNA fragment may be very useful in routine identification of Cl. m. subsp. michiganensis from all other bacteria isolated from tomato seeds and may be an alternative tool in diagnostics, especially for the quarantine services.
Clavibacter michiganensis subsp. michiganensis jest bardzo groźnym patogenem kwarantannowym pomidora, wywołującym chorobę zwaną bakteryjnym rakiem pomidora. Bakteria ta łatwo przenosi się z zakażonymi nasionami i sadzonkami. Pomimo opracowania różnych technik diagnostycznych do jej wykrywania, nadal stanowi duży problem w diagnozowaniu chorób pomidora. Powszechnie stosowane w Polsce wykrywanie tej bakterii, bazujące na testach enzymoimmunologicznych (ELISA), immunofluorescencyjnych, hodowli na pożywkach półselektywnych i testach biologicznych, jest często zawodne ze względu na duże serologiczne zróżnicowanie poszczególnych szczepów tej bakterii, obecność wielu bakterii saprofitycznych, tworzących kolonie nie różniące się morfologicznie od kolonii właściwych dla Cl. m. subsp. michiganensis, czy też mało przydatne ze względu na długi czas trwania testu i obecność infekcji latentnych. Jedną z nowoczesnych metod wykrywania i identyfikacji bakterii jest amplifikacja fragmentów DNA charakterystycznych dla jej genomu przy pomocy reakcji PCR. Metoda ta dopiero zaczyna być powszechnie stosowana w diagnostyce bakterii (nieliczne doniesienia, w większości z ostatnich trzech lat). Opracowana metoda identyfikacji Cl. m. subsp. michiganensis spośród różnych bakterii saprofitycznych obecnych na nasionach pomidora, charakteryzuje się bardzo dużą czułością (do wykrycia Cl. m. subsp. michiganensis wystarczy 200-300 bakterii / 1 ml), specyficznością (produkty amplifikacji DNA bakteryjnego o oczekiwanej długości 614 bp, uzyskiwano tylko w próbkach, w których znajdował się DNA z Cl. m. subsp. michiganensis) oraz szybkością (jeden dzień, wraz z izolacją DNA bakteryjnego). Ponadto opracowana metoda charakteryzuje się bardzo niskimi kosztami, gdyż cala reakcja amplifikacji DNA zachodzi w objętości 10 μI (4 μI zawiesiny DNA i 6 μI mieszaniny reakcyjnej), co sprawia, że stosowanie tej metody w powszechnej diagnostyce chorób bakteryjnych będzie coraz częstsze i będzie ona coraz bardziej konkurencyjna w stosunku do innych, obecnie stosowanych.

Źródło:

Journal of Plant Protection Research; 1997, 37, 1-2
1427-4345

Pojawia się w:

Journal of Plant Protection Research

Dostawca treści:

Biblioteka Nauki

/5

Artykuł

Zmień widok

• na półce

Skocz do pozycji: 79.

Tytuł:

Molecular characterization of the first internal transcribed spacer of rDNA of Parabronema skrjabini for the first time in sheep

Autorzy:

Hasheminasab, S.S.

Powiązania:

https://bibliotekanauki.pl/articles/6198.pdf

Data publikacji:

2015

Wydawca:

Polskie Towarzystwo Parazytologiczne

Tematy:

molecular characteristics
nematode
rDNA
Parabronema skrjabini
food-borne parasite
parasitic disease
sheep
polymerase chain reaction
Iran

Opis:

Parabronema skrjabini is a spirurid nematode of the family Habronematidae that lives in the abomasum of ruminants such as sheep and goats. The purpose of this study was to investigate the molecular aspects of Parabronema skrjabini in sheep. The worms were collected from sheep in Sanandaj (west of Iran). The first internal transcribed spacer (ITS) of ribosomal DNA (rDNA) nucleotide fragments of Parabronema skrjabini were amplified by polymerase chain reaction (PCR) using two pairs of specific primers (Para-Ir-R and Para-Ir-F). ITS1 homology in the sequence of this study was 69% compared with the sequence data in GenBank. To our knowledge, this is the first study in the world exploring the genetic diversity of P. skrjabini in sheep based on ITS1.

Źródło:

Annals of Parasitology; 2015, 61, 4
0043-5163

Pojawia się w:

Annals of Parasitology

Dostawca treści:

Biblioteka Nauki

/5

Artykuł

Zmień widok

• na półce

Skocz do pozycji: 80.

Tytuł:

Detection and identification of banned processed animal protein in feedingstuffs by microscopic and PCR methods

Autorzy:

Weiner, A.
Golebiowska, A.
Paprocka, I.
Kwiatek, K.

Powiązania:

https://bibliotekanauki.pl/articles/30136.pdf

Data publikacji:

2012

Wydawca:

Polska Akademia Nauk. Czytelnia Czasopism PAN

Tematy:

detection
identification
processed animal protein
microscopic method
polymerase chain reaction
feeding stuff
meat meal
bone meal

Opis:

The aim of the study was to present the results of comparative evaluation of the usefulness of PCR and microscopic methods for the detection of Processed Animal Protein (PAP) in feedingstuffs. In the validation study, the limit of the detection for PCR was determined on 0.05% for beef, 0.1% for pork and 0.2% for poultry meat and bone meal (MBM). Among 62 doubtful samples of feedingstuffs examined by microscopic method 41 (66.13%) were found as positive. Based on the results obtained with the use of the microscopic and PCR methods it is possible to state that the molecular biology methods can, at present, be used as a supplementary method in PAP detection.

Źródło:

Polish Journal of Veterinary Sciences; 2012, 15, 1
1505-1773

Pojawia się w:

Polish Journal of Veterinary Sciences

Dostawca treści:

Biblioteka Nauki

/5

Artykuł

Zmień widok

• na półce

Skocz do pozycji: 81.

Tytuł:

Genetic similarity of chosen Syringa species determined by the ISSR-PCR technique

Autorzy:

Rzepka-Plevnes, D
Smolik, M.
Tanska, K.

Powiązania:

https://bibliotekanauki.pl/articles/41611.pdf

Data publikacji:

2006

Wydawca:

Polska Akademia Nauk. Instytut Dendrologii PAN

Tematy:

Przelewice Dendrological Garden
genetic variability
Syringa
lilac
genetic diversity
taxonomy
ISSR technique
polymerase chain reaction

Opis:

Inter-simple sequence repeat (ISSR) amplification was used to analyze polymorphism of microsatellite sequences in the lilacs genom and to evaluate genetic diversity among seven lilacs species (Syringa × prestoniae McKelvey., S. reflexa K.C. Schneid., S. villosa Vahl, S. × chinensis Willd., S. meyeri K.C.Schneid., S. vulgaris L.and S. reticulata (Blume) var. amurensis (Rupr.)). The plant material was originated from the collection of Dendrological Garden in Przelewice.A total of 30 primers, containing different simple sequence repeat motifs were tested for amplification.Out of the 30 primers only 13 gave interpretable banding patterns in all lilacs species.A total of 182 ISSR fragments were generated with 13 primers of which 109 (60%) were polymorphic and 57 (31.2%) species-specific. ISSR–PCR with genomic DNAs of the showed lilacs yielded DNA fragmets ranging form 2200 to 123 bp in size.Species-specific ISSR fragments were detected for each lilacs accessions.UPGMA cluster analysis was used to construct a dendrogram and to estimate the genetic distances between lilacs species.The ISSR-based phylogeny was generally consistent with Syringa taxonomy based on morphological and phenological evidence.

Źródło:

Dendrobiology; 2006, 56; 61-67
1641-1307

Pojawia się w:

Dendrobiology

Dostawca treści:

Biblioteka Nauki

/5

Artykuł

Zmień widok

• na półce

Skocz do pozycji: 82.

Tytuł:

Temporal expression of three conserved putative microRNAs in response of Citrus × Limon to Xanthomonas citri subsp. citri and Xanthomonas fuscans subsp. Aurantifolii

Autorzy:

Alizadeh, M.
Askari, H.
Najafabadi, M.S.

Powiązania:

https://bibliotekanauki.pl/articles/81264.pdf

Data publikacji:

2017

Wydawca:

Polska Akademia Nauk. Czytelnia Czasopism PAN

Tematy:

lemon
fruit canker
bacterial disease
Xanthomonas
microRNA
plant-pathogen interaction
reserve transcript polymerase chain reaction

Źródło:

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2017, 98, 3
0860-7796

Pojawia się w:

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

Dostawca treści:

Biblioteka Nauki

/5

Artykuł

Zmień widok

• na półce

Skocz do pozycji: 83.

Tytuł:

Large-scale analysis of sequence tags in Xp11.4-11.3 and evaluation of candidate genes for X-linked ocular diseases

Autorzy:

Pusch, C M

Powiązania:

https://bibliotekanauki.pl/articles/2041943.pdf

Data publikacji:

2001

Wydawca:

Polska Akademia Nauk. Czytelnia Czasopism PAN

Tematy:

X chromosome
man
pseudogene
polymerase chain reaction
X-linked ocular disease
night vision
genetic disease

Źródło:

Journal of Applied Genetics; 2001, 42, 1; 89-102
1234-1983

Pojawia się w:

Journal of Applied Genetics

Dostawca treści:

Biblioteka Nauki

/5

Artykuł

Zmień widok

• na półce

Skocz do pozycji: 84.

Tytuł:

Gene expression and peptide localization for LH-hCG receptor in porcine small and large luteal cells: possible regulation by opioid peptides

Autorzy:

Kaminski, T.
Gawronska, B.
Derecka, K.
Okrasa, S.
Przala, J.

Powiązania:

https://bibliotekanauki.pl/articles/69878.pdf

Data publikacji:

2000

Wydawca:

Polskie Towarzystwo Fizjologiczne

Tematy:

reverse transcription
opioid peptide
gene expression
immunocytochemistry
polymerase chain reaction
peptide localization
porcine luteal cell

Źródło:

Journal of Physiology and Pharmacology; 2000, 51, 2
0867-5910

Pojawia się w:

Journal of Physiology and Pharmacology

Dostawca treści:

Biblioteka Nauki

/5

Artykuł

Zmień widok

• na półce

Skocz do pozycji: 85.

Tytuł:

RCD1 regulation and recognition of poly(ADP-ribosyl)ation in Arabidopsis

Autorzy:

Vainonen, J.
Shapiguzov, A.
Wrzaczek, M.
Kangasjarvi, J.

Powiązania:

https://bibliotekanauki.pl/articles/80058.pdf

Data publikacji:

2013

Wydawca:

Polska Akademia Nauk. Czytelnia Czasopism PAN

Tematy:

conference
abiotic stress
biotic stress
plant stress
stress response
reactive oxygen species
poly[ADP ribose] polymerase
Arabidopsis

Źródło:

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2013, 94, 2
0860-7796

Pojawia się w:

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

Dostawca treści:

Biblioteka Nauki

/5

Artykuł

Zmień widok

• na półce

Skocz do pozycji: 86.

Tytuł:

Unidirectional orientation of twelve expressed tagged sites within 4o kb of human chromosomal region 22q13.1

Autorzy:

Pusch, C
Wang, Z.
Roe, B.
Blin, N.

Powiązania:

https://bibliotekanauki.pl/articles/2048293.pdf

Data publikacji:

1998

Wydawca:

Polska Akademia Nauk. Czytelnia Czasopism PAN

Tematy:

RNA
human chromosome
chromosome
hybridization
genetic change
linear map
polymerase chain reaction
DNA
cosmid
cDNA
transcriptional orientation

Opis:

The single copy sequence D22S16 from human chromosomal region 22q13.1 that carries a putative conserved gene, was used to probe a chromosome 22-specific cosmid library. Genomic sequencing of one positive, 40 kb long cosmid (C1155) revealed a hereto unmapped gene (a subunit of DNA-dependent RNA polymerase II, POLR2F), a SOX9-related sequence and 12 expressed sequence tags. Although not parts of one consecutive gene, all 12 ESTs and, in addition, the polymerase gene are oriented in the same transcriptional direction within the genomic sequence represented by cosmid C1155.

Źródło:

Journal of Applied Genetics; 1998, 39, 2; 199-204
1234-1983

Pojawia się w:

Journal of Applied Genetics

Dostawca treści:

Biblioteka Nauki

/5

Artykuł

Zmień widok

• na półce

Skocz do pozycji: 87.

Tytuł:

Genotyping of bovine beta-lactoglobulin [LGB] by PCR-SSCP technique

Autorzy:

Kaminski, S
Zabolewicz, T

Powiązania:

https://bibliotekanauki.pl/articles/2046615.pdf

Data publikacji:

1997

Wydawca:

Polska Akademia Nauk. Czytelnia Czasopism PAN

Tematy:

AB genotype
SSCP method
cattle
genotype
AA genotype
BB genotype
beta-lactoglobulin
polymerase chain reaction
allele
identification

Opis:

A new method facilitating the identification of the two most common alleles (A and B) of the bovine beta-lacoglobulin (LGB) gene is described. The method is based on two steps: PCR amplification of 240 bp fragment of LGB gene followed by the single stranded conformation polymorphism (SSCP) detection. AA, AB and BB genotypes of LGB were identified with this technique. The PCR-SSCP is simple, accurate and relatively inexpensive. Additionally, this method has a potential to detect new variants within the amplified gene fragment.

Źródło:

Journal of Applied Genetics; 1997, 38, 4; 471-476
1234-1983

Pojawia się w:

Journal of Applied Genetics

Dostawca treści:

Biblioteka Nauki

/5

Artykuł

Zmień widok

• na półce

Skocz do pozycji: 88.

Tytuł:

A simple method for extracting DNA from rhododendron plants infected with Phytophthora spp. for use in PCR

Autorzy:

Trzewik, A.
Nowak, K.J.
Orlikowska, T.

Powiązania:

https://bibliotekanauki.pl/articles/66480.pdf

Data publikacji:

2016

Wydawca:

Polska Akademia Nauk. Czytelnia Czasopism PAN

Tematy:

simple method
DNA extraction
rhododendron
leaf
plant infection
Phytophthora
polymerase chain reaction
detection
real-time PCR method

Opis:

Among the numerous protocols that describe the extraction of DNA, those relating to the isolation of DNA from infected plants, are rare. This study describes a rapid and reliable method of extracting a high quality and quantity of DNA from rhododendron leaves artificially infected with Phytophthora cactorum, P. cambivora, P. cinnamomi, P. citrophthora, and P. plurivora. The use of the modified Doyle and Doyle protocol (1987) allowed us to obtain high quantity and quality DNA (18.26 μg from 100 mg of the fresh weight of infected leaves at the ratios of A260/280 and A260/230 – 1.83 and 1.72, respectively), suitable for conventional polymerase chain reaction (PCR) and real-time PCR amplifications.

Źródło:

Journal of Plant Protection Research; 2016, 56, 1
1427-4345

Pojawia się w:

Journal of Plant Protection Research

Dostawca treści:

Biblioteka Nauki

/5

Artykuł

Zmień widok

• na półce

Skocz do pozycji: 89.

Tytuł:

A small-scale survey of hantavirus in mammals from eastern Poland

Autorzy:

Wojcik-Fatla, A.
Zajac, V.
Knap, J.P.
Sroka, J.
Cisak, E.
Sawczyn, A.
Dutkiewicz, J.

Powiązania:

https://bibliotekanauki.pl/articles/51577.pdf

Data publikacji:

2013

Wydawca:

Instytut Medycyny Wsi

Tematy:

hantavirus
Bunyaviridae
mammal
small mammal
Microtus agrestis
Myodes glareolus
Sorex araneus
epidemiology
polymerase chain reaction
flood
Polska

Opis:

Samples of 30 dead small mammals each were collected on area ‘A’ located in eastern Poland which is exposed to flooding by the Vistula river, and on the area ‘B’, also located in eastern Poland but not exposed to flooding. Kidneys and livers of the mammals were examined by the PCR and nested PCR methods for the presence of hantavirus RNA. Out of 7 species of small mammals examined, the presence of hantaviruses was detected in 4 of them. Hantavirus prevalence was low in Apodemus agrarius (2.6%), the most numerous mammal species, whereas in the remaining 3 positive species (Microtus agrestis, Myodes glareolus, Sorex araneus) this was 12.5–100%. The presence of hantaviruses was detected only in the animals found on area ‘A’ exposed to flooding, and their prevalence was statistically greater compared to area ‘B’ not exposed to flooding (16.7% vs. 0%, p=0.0345). The overall positivity of the examined small mammals population from the areas ‘A’ and ‘B’ was 8.3%. The sequence analysis of the samples positive for hantavirus proved that the amplified products showed 77–86% homology with the L segment sequence of hantavirus Fusong-Mf-731 isolated from Microtus fortis in China. The presented study is the first to demonstrate the occurrence of hantavirus infection in small mammals from eastern Poland, and the first to demonstrate the significant relationship between flooding and the prevalence of hantaviruses in small mammals.

Źródło:

Annals of Agricultural and Environmental Medicine; 2013, 20, 2
1232-1966

Pojawia się w:

Annals of Agricultural and Environmental Medicine

Dostawca treści:

Biblioteka Nauki

/5

Artykuł

Zmień widok

• na półce

Skocz do pozycji: 90.

Tytuł:

Molecular and serological diagnosis of Borrellia burgdorferi infection among patients with diagnosed erythema migrans

Autorzy:

Kondrusik, M
Grygorczuk, S.
Skotarczak, B.
Wodecka, B.
Rymaszewska, A.
Pancewicz, S.
Zajkowska, J.
Swierzbinska, R.
Hermanowska-Szpakowicz, T.

Powiązania:

https://bibliotekanauki.pl/articles/51856.pdf

Data publikacji:

2007

Wydawca:

Instytut Medycyny Wsi

Tematy:

human disease
erythema migrans phase
patient
diagnosis
ELISA test
polymerase chain reaction
serological diagnosis
Borrelia burgdorferi
borreliosis

Opis:

The aim of the study was to assess the frequency of Borrelia burgdorferi DNA detection in the blood and urine of patients diagnosed with erythema migrans, and compare the results of PCR-based methods with ELISA methodology. The latter was used to detect serum antibodies against Borrelia burgdorferi of the IgM and IgG classes, before and after antibiotic therapy. The study included 86 patients hospitalized in the Department of Infectious Diseases and Neuroinfections in the Medical Academy in Białystok, diagnosed with the erythema migrans phase of Lyme borreliosis. Examinations were carried out twice: the fi rst at the moment of diagnosis (Trial 1), the second after 4 weeks of antibiotic therapy. The study showed that antibiotic therapy in the early phase of borreliosis does not decrease the sensitivity of PCR and that after 4 weeks of therapy (Trial 2), spirochete DNA is still detectable in most patients (45/86). There was no correlation between detectability of spirochete DNA and the presence of antibodies against B. burgdorferi s.l. (assessed by ELISA) during the course of erythema migrans. The largest percentage of positive results in the detection of B. burgdorferi s.l. DNA was observed in patients who simultaneously possessed IgM and IgG antibodies against B. burgdorferi, while the lowest percentage of PCR positive results was among patients with only IgM antibodies.

Źródło:

Annals of Agricultural and Environmental Medicine; 2007, 14, 2
1232-1966

Pojawia się w:

Annals of Agricultural and Environmental Medicine

Dostawca treści:

Biblioteka Nauki

/5

Artykuł

Zmień widok

• na półce

Skocz do pozycji: 91.

Tytuł:

Evaluation of methods for Erwinia amylovora detection

Autorzy:

Kaluzna, M.
Pulawska, J.
Mikicinski, A.

Powiązania:

https://bibliotekanauki.pl/articles/1986.pdf

Data publikacji:

2013

Wydawca:

Instytut Ogrodnictwa

Tematy:

Erwinia amylovora
fire blight
real-time polymerase chain reaction
LAMP technique
DNA sequence
detection method
disease control

Źródło:

Journal of Horticultural Research; 2013, 21, 2
2300-5009

Pojawia się w:

Journal of Horticultural Research

Dostawca treści:

Biblioteka Nauki

/5

Artykuł

Zmień widok

• na półce

Skocz do pozycji: 92.

Tytuł:

Analysis of the epidemiological factors influencing vulpine trichinellosis in ecologically different regions of Slovakia

Autorzy:

Hurnikova, Z
Bartkova, D.
Dubinsky, P.

Powiązania:

https://bibliotekanauki.pl/articles/839915.pdf

Data publikacji:

2006

Wydawca:

Polskie Towarzystwo Parazytologiczne

Tematy:

red fox
parasite
Trichinella
epidemiological factor
trichinellosis
Vulpes vulpes
parasitology
polymerase chain reaction
animal disease
Slovakia

Opis:

Introduction. In the Slovak Republic, trichinellosis circulates almost exclusively in the sylvatic cycle, with main reservoir host red fox and wild boar and sporadic occurrence of human outbreaks. A detailed study was performed in five ecologically different regions of eastern Slovakia with more profound regard to eco-geographical and anthropogenic influences to natural fox habitat. Material and methods. In total of 689 red foxes (Vulpes vulpes) hunted in selected regions in 2005/2006 was examined using artificial digestion method. Larvae obtained from infected samples were on the species level characterised using multiplex PCR analysis. Results. The study revealed a total prevalence of 15.6%, with most frequent occurrence of infected foxes in the mountain of the Volovské Vrchy (25.2%) where both human habitation and fox population are very dense. High prevalence rates were found in the Košická Kotlina Basin (19.6%) with urbanised landscape, concentrated human activities and low fox population and in national park of the High Tatras (15.8%) where the inhabitants and fox population are relatively low. In the remote localities of the Nízke Beskydy Highlands that represent ideal fox habitat free of any human impact, 14.2% of foxes harboured Trichinella larvae. The lowest occurrence of infected foxes (6.9%) was found in agrarian areas of the Východoslovenská Nížina Lowland, with relatively low inhabitants and fox population density. In all localities Trichinella britovi was the most important etiological agent of sylvatic trichinellosis.

Źródło:

Annals of Parasitology; 2006, 52, 3
0043-5163

Pojawia się w:

Annals of Parasitology

Dostawca treści:

Biblioteka Nauki

/5

Artykuł

Zmień widok

• na półce

Skocz do pozycji: 93.

Tytuł:

Role of polymorphisms TNF.- alpha gene in the development of recurrent miscarriage after in vitro fertilization in the residentS of Odessa region of Ukraine

Autorzy:

Golovatyuk, K. P.

Powiązania:

https://bibliotekanauki.pl/articles/765441.pdf

Data publikacji:

2017

Wydawca:

Uniwersytet Mikołaja Kopernika w Toruniu. Wydział Nauk o Ziemi i Gospodarki Przestrzennej. Katedra Kultury Fizycznej

Tematy:

recurrent miscarriage, in vitro fertilization, tumor necrosis factor-α r, inheritance, polymorphism, genotype, polymerase chain reaction

Opis:

Golovatyuk K. P. Role of polymorphisms TNF.- alpha gene in the development of recurrent miscarriage after in vitro fertilization in the residentS of Odessa region of Ukraine. Journal of Education, Health and Sport. 2017;7(1):323 - 332. eISSN 2391-8306. DOI http://dx.doi.org/10.5281/zenodo.265805http://ojs.ukw.edu.pl/index.php/johs/article/view/4218    The journal has had 7 points in Ministry of Science and Higher Education parametric evaluation. Part B item 754 (09.12.2016).754 Journal of Education, Health and Sport eISSN 2391-8306 7© The Author (s) 2017;This article is published with open access at Licensee Open Journal Systems of Kazimierz Wielki University in Bydgoszcz, PolandOpen Access. This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and source are credited. This is an open access article licensed under the terms of the Creative Commons Attribution Non Commercial License(http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted, non commercial use, distribution and reproduction in any medium, provided the work is properly cited.This is an open access article licensed under the terms of the Creative Commons Attribution Non Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted, non commercial use, distribution and reproduction in any medium, provided the work is properly cited.The authors declare that there is no conflict of interests regarding the publication of this paper.Received: 02.01.2017. Revised 16.01.2017. Accepted: 24.01.2017. UDK 618.177-089.888.11:618.39-0796:575.162 ROLE OF POLYMORPHISMS TNF- alpha GENE IN THE DEVELOPMENT OF recurrent miscarriage after in vitro fertilization in THE  residentS of Odessa region of Ukraine K. P. Golovatyuk Odessa National medical University, Ukraine; nosenko.olena@gmail.com;  info@gameta.od.ua SummaryThe frequency of genotypes and allelic variants of the TNF-α gene (-238 G> A, -308 G> A, -1031 T > C), depending on the reproductive status and evaluation of its association with recurrent miscarriage (RM) in cycles in vitro fertilization (IVF) has been studied. The residents of the Odessaregion of Ukrainehave been under examination. It has been revealed that typing of SNPs of genes of the immune response TNF-α (rs1799964) and TNF-α (rs1800629) may be used as an early diagnostic method and pregravida prediction of reproductive losses in infertile women with RM after IVF. Genotypes AA -308G> A, (GA + AA) -308G> A and TC-1031C, (TC + CC) -1031C TNF-α gene were statistically significant associated with an increased risk of RM after IVF.Key words: recurrent miscarriage, in vitro fertilization, tumor necrosis factor-α r, inheritance, polymorphism, genotype, polymerase chain reaction.

Źródło:

Journal of Education, Health and Sport; 2017, 7, 1
2391-8306

Pojawia się w:

Journal of Education, Health and Sport

Dostawca treści:

Biblioteka Nauki

/5

Artykuł

Zmień widok

• na półce

Skocz do pozycji: 94.

Tytuł:

Analysis of the epidemiological factors influencing vulpine trichinellosis in ecologically different regions of Slovakia

Autorzy:

Hurnikova, Z.
Bartkova, D.
Dubinsky, P.

Powiązania:

https://bibliotekanauki.pl/articles/2144345.pdf

Data publikacji:

2006

Wydawca:

Polskie Towarzystwo Parazytologiczne

Tematy:

red fox
parasite
Trichinella
epidemiological factor
trichinellosis
Vulpes vulpes
parasitology
polymerase chain reaction
animal disease
Slovakia

Opis:

Introduction. In the Slovak Republic, trichinellosis circulates almost exclusively in the sylvatic cycle, with main reservoir host red fox and wild boar and sporadic occurrence of human outbreaks. A detailed study was performed in five ecologically different regions of eastern Slovakia with more profound regard to eco-geographical and anthropogenic influences to natural fox habitat. Material and methods. In total of 689 red foxes (Vulpes vulpes) hunted in selected regions in 2005/2006 was examined using artificial digestion method. Larvae obtained from infected samples were on the species level characterised using multiplex PCR analysis. Results. The study revealed a total prevalence of 15.6%, with most frequent occurrence of infected foxes in the mountain of the Volovské Vrchy (25.2%) where both human habitation and fox population are very dense. High prevalence rates were found in the Košická Kotlina Basin (19.6%) with urbanised landscape, concentrated human activities and low fox population and in national park of the High Tatras (15.8%) where the inhabitants and fox population are relatively low. In the remote localities of the Nízke Beskydy Highlands that represent ideal fox habitat free of any human impact, 14.2% of foxes harboured Trichinella larvae. The lowest occurrence of infected foxes (6.9%) was found in agrarian areas of the Východoslovenská Nížina Lowland, with relatively low inhabitants and fox population density. In all localities Trichinella britovi was the most important etiological agent of sylvatic trichinellosis.

Źródło:

Wiadomości Parazytologiczne; 2006, 52, 3; 213-218
0043-5163

Pojawia się w:

Wiadomości Parazytologiczne

Dostawca treści:

Biblioteka Nauki

/5

Artykuł

Zmień widok

• na półce

Skocz do pozycji: 95.

Tytuł:

Dissecting the action of H2O2 after its metabolic formation in chloroplasts

Autorzy:

Jaspert, N.
Balazadeh, S.
Arif, M.
Muller-Rober, B.
Maurino, V.

Powiązania:

https://bibliotekanauki.pl/articles/80107.pdf

Data publikacji:

2013

Wydawca:

Polska Akademia Nauk. Czytelnia Czasopism PAN

Tematy:

conference
reactive oxygen species
toxic cellular metabolite
signalling molecule
Arabidopsis thaliana
glycolate
chloroplast
real-time polymerase chain reaction

Źródło:

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2013, 94, 2
0860-7796

Pojawia się w:

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

Dostawca treści:

Biblioteka Nauki

/5

Artykuł

Zmień widok

• na półce

Skocz do pozycji: 96.

Tytuł:

Application of a duplex-PCR for detection of cows' milk in goats' milk

Autorzy:

Kotowicz, M
Adamczyk, E.
Bania, J.

Powiązania:

https://bibliotekanauki.pl/articles/51721.pdf

Data publikacji:

2007

Wydawca:

Instytut Medycyny Wsi

Tematy:

cow milk
human disease
goat milk
food
allergy
polymerase chain reaction
species identification
milk-derived product
detection
milk

Opis:

A duplex-PCR method, with 2 pairs of primers recognizing sequences of mitochondrial D-loop region, was developed to identify cows’ milk in the milk of goats. The PCR was shown to be specifi c and sensitive, enabling the detection of less than 1% of cows’ milk added to the milk of goats. Simultaneous use of a primer pair for goats’ and cows’ mitochondrial DNA fragment prevented false negative results. The method was applied to track the presence of cow DNA in goat milk available on the Polish market. A total of 54 milk samples from 3 Polish (34) and one foreign producer (20) were examined. In 33 samples, cow DNA was detected, while 21 samples, including all of the 20 samples from foreign producers, produced the goat-specific product only.

Źródło:

Annals of Agricultural and Environmental Medicine; 2007, 14, 2
1232-1966

Pojawia się w:

Annals of Agricultural and Environmental Medicine

Dostawca treści:

Biblioteka Nauki

/5

Artykuł

Zmień widok

• na półce

Skocz do pozycji: 97.

Tytuł:

The prevalence of Ehrlichia canis, Anaplasma platys and Babesia spp. in dogs in Nueva Ecija, Philippines based on multiplex polymerase chain reaction (mPCR) assay

Autorzy:

Corales, J.M.I.
Viloria, V.V.
Venturina, V.M.
Mingala, C.N.

Powiązania:

https://bibliotekanauki.pl/articles/6624.pdf

Data publikacji:

2014

Wydawca:

Polskie Towarzystwo Parazytologiczne

Tematy:

prevalence
Ehrlichia canis
Anaplasma platys
Babesia
dog
tick-borne disease
Nueva Ecija province
Philippines
multiplex polymerase chain reaction

Opis:

The aim of the study was to determine the prevalence of Ehrlichia canis, Anaplasma platys and Babesia spp. in dogs. It describes the practice of veterinarians in detecting tick-borne diseases in Nueva Ecija, Philippines. Seventy blood samples were collected and were subjected to multiplex PCR for the detection of E. canis, Babesia spp. and A. platys. The prevalence of babesiosis is the highest in Cabanatuan City (2/10), while a 10% prevalence (1/10) was observed in Science City of Muñoz, Talavera and Sta. Rosa. E. canis were only detected in Cabanatuan City. However, no anaplasmosis was detected in any area. The prevalence of babesiosis and ehrlichiosis in Nueva Ecija is 7.14% (5/70) and 2.85% (2/70) respectively. In addition, 70% (7/10) of the Nueva Ecija veterinary practitioners encountered cases of suspected ehrlichiosis in their practice. The diagnosis of ehrlichiosis is based primarily on presented clinical signs and complete blood counts, which include a platelet count. Of the 10 respondents, half utilized test kits while 90% interpreted blood samples. Meanwhile, only 60% of the respondents used an ELISA test kit for ehrlichiosis. For some practitioners, the main reason for not utilizing a kit is the high cost. None of the respondents had previously attended cases of suspected anaplasmosis. Only one respondent diagnosed a case of babesiosis by blood smear microscopy.

Źródło:

Annals of Parasitology; 2014, 60, 4
0043-5163

Pojawia się w:

Annals of Parasitology

Dostawca treści:

Biblioteka Nauki

/5

Artykuł

Zmień widok

• na półce

Skocz do pozycji: 98.

Tytuł:

Analysis of occurrence of virulence genes among Yersinia enterocolitica isolates belonging to different biotypes and serotypes

Autorzy:

Kot, B
Piechota, M.
Jakubczak, A.

Powiązania:

https://bibliotekanauki.pl/articles/32221.pdf

Data publikacji:

2010

Wydawca:

Polska Akademia Nauk. Czytelnia Czasopism PAN

Tematy:

virulence gene
occurrence
Yersinia enterocolitica
isolate
biotype
serotype
polymerase chain reaction
ystB gene
myfA gene
man
pig
isolation

Opis:

The 150 Y. enterocolitica strains isolated from humans and from pigs belonged to biotypes 4 (68.7%), 1A (18.7%) and 2 (4%), or were biochemically untypeable (8.6%). Biotype 4 was comprised of Y. enterocolitica strains representing serotype 0:3, within biotype 1A the strains either belonged to serotypes 0:5 and 0:6 or were untypeable, and biotype 2 was represented by the strains of serotype 0:9. The strains which were biochemically untypeable belonged to serotypes 0:5, 0:6 and 0:3. Among the strains tested there also were those of an unidentified biotype and serotype. Nearly all the strains of biotype 1A represented genotype ystB+myfA+, and few belonged to genotype ystB+. The presence of the ystB gene in the strains of biotype 1A and only occasional occurrence of the gene in the other biotypes makes ystB a distinguishing marker of biotype 1A. The strains of genotype ystA+ail+myfA+yadA+ predominated in biotype 4 (serotype 0:3). The strains of biotype 2 (serotype 0:9) represented genotype ystA+ail+myfA+, and the plasmid yadA gene was detected in some of them. Within the group of biochemically untypeable strains ystB- and myfA-specific PCR products were mainly obtained. The genotypes determined for the tested biotypes and serotypes of Y. enterocolitica, based upon the selected genes of virulence, can be applied as distinguishing markers and indicators of the potential virulence of Y. enterocolitica strains, excluding bioserotyping.

Źródło:

Polish Journal of Veterinary Sciences; 2010, 13, 1; 13-19
1505-1773

Pojawia się w:

Polish Journal of Veterinary Sciences

Dostawca treści:

Biblioteka Nauki

/5

Artykuł

Zmień widok

• na półce

Skocz do pozycji: 99.

Tytuł:

Reduced expression of AURKA in peripheral blood of breast cancer patients

Autorzy:

Goh, L.P.W.
See, E.U.H.
Chua, K.H.
Lee, P.-C.

Powiązania:

https://bibliotekanauki.pl/articles/81279.pdf

Data publikacji:

2018

Wydawca:

Polska Akademia Nauk. Czytelnia Czasopism PAN

Tematy:

breast cancer
patient
Aurora kinase
gene expression
peripheral blood
cell cycle
quantitative real-time polymerase chain reaction

Źródło:

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2018, 99, 1
0860-7796

Pojawia się w:

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

Dostawca treści:

Biblioteka Nauki

/5

Artykuł

Zmień widok

• na półce

Skocz do pozycji: 100.

Tytuł:

Inhibition of poly(ADP-ribose) polymerase activity affects its subcellular localization and DNA strand break rejoining

Autorzy:

Ryabokon, Nadezhda
Cieślar-Pobuda, Artur
Rzeszowska-Wolny, Joanna

Powiązania:

https://bibliotekanauki.pl/articles/1040571.pdf

Data publikacji:

2009

Wydawca:

Polskie Towarzystwo Biochemiczne

Tematy:

DNA strand break rejoining
efficiency of PARP inhibition
PARP foci
poly(ADP-ribose) polymerase (PARP)
PARP inhibitors

Opis:

Poly(ADP-ribose) polymerase (PARP) plays a crucial role in DNA repair. Modulation of its activity by stimulation or inhibition is considered as a potentially important strategy in clinical practice, especially to sensitize tumor cells to chemo- and radiotherapy through inhibition of DNA repair. Here we studied the effect of the three PARP inhibitors, 5-iodo-6-amino-benzopyrone (INH2BP), 1,5-isoquinolinediol (1,5-dihydroxyisoquinolinediol (1,5-IQD) and 8-hydroxy-2-methylquinazolin-4-[3H]one (NU1025), and for two of them the efficiency in slowing the rejoining of DNA strand breaks induced by H2O2 was compared. Inhibition of PARP changed its intranuclear localization markedly; cells exposed to the inhibitor NU1025 showed a significant tendency to accumulate PARP in large foci, whereas in untreated cells its distribution was more uniform. The speed and efficiency of rejoining of H2O2-induced DNA strand breaks were lower in cells incubated with a PARP inhibitor, and the kinetics of rejoining were modulated in a different manner by each inhibitor. At a concentration of 100 µM the efficiency of the inhibitors could be ranked in the order NU1025 > IQD > INH2BP. The two first compounds were able to decrease the overall PARP activity below the level detected in control cells, while INH2BP showed up to 40% PARP activity after exposure to H2O2.

Źródło:

Acta Biochimica Polonica; 2009, 56, 2; 243-248
0001-527X

Pojawia się w:

Acta Biochimica Polonica

Dostawca treści:

Biblioteka Nauki

/5

Artykuł

Zmień widok

• na półce