Skocz do pozycji: 1.

Tytuł:

Testing of the synchronous fluorescence in application to used lubricate oil characterisation

Autorzy:

Baszanowska, E.
Otremba, Z.

Powiązania:

https://bibliotekanauki.pl/articles/243560.pdf

Data publikacji:

2016

Wydawca:

Instytut Techniczny Wojsk Lotniczych

Tematy:

marine fleet exploitation
lubricate oil
fluorescence spectroscopy
synchronous fluorescence spectra

Opis:

Reliability of the ship propulsion system depends, inter alia, on the quality of lubricate oils. Research indicates that exploitive features of oil are reduced with it runtime in the ship engine circuit. At the present the basic parameters representing exploitive features of oil are: water content, total acid number and total base number or viscosity vs. temperature, anti-wear performance, further: content of additives and their concentrations, reaction products, and other contaminating substances, also magnetically separated large ferromagnetic particles, the size, the shape, the composition and concentration of the abnormal wear particles. Additional properties of oil are described based on atomic emission spectroscopy, granulometry of separated from the lubricant sample by magnets and gravity, permeability changes, as well as rheometric and tribometric characteristics. Complexity of composition of lubricate oil raises the idea to use changes of relative content of any compounds for characterization of oil exploitive features. Fluorescence method is possible to use for oil properties characterisation due to the presence mono and polycyclic compounds in oil that induces fluorescence phenomenon. Therefore identification the fluorescence spectra when exploitation time of oil increases could be novel indicator to monitor of lubricate oil exploitive quality. In this study we present changes of oil fluorescence expressed by synchronous spectra, taking into account working time of exemplary lubricate oil in the ship engine.

Źródło:

Journal of KONES; 2016, 23, 2; 39-44
1231-4005
2354-0133

Pojawia się w:

Journal of KONES

Dostawca treści:

Biblioteka Nauki

/5

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Skocz do pozycji: 2.

Tytuł:

Spectroscopic methods in application to oil pollution detection in the sea

Autorzy:

Baszanowska, E.
Otremba, Z.

Powiązania:

https://bibliotekanauki.pl/articles/248141.pdf

Data publikacji:

2012

Wydawca:

Instytut Techniczny Wojsk Lotniczych

Tematy:

marine transport
oil pollution
spectroscopic methods
fluorescence
time-resolved fluorescence

Opis:

The escalating maritime transport as well as offshore crude oil exploitation and pipeline transportation leads to the increase of feasibility of the discharge of petroleum substances to the sea environment events. Therefore, it is necessary to rapidly detect of oil pollution to minimize the extent of contamination in the seawater. The aim of this study is to discuss the assumptions of the optical methods using for detection of oil pollution in the seawater. We consider the different approaches of oil-in-water detection using spectral active and passive techniques with emphasis on fluorescence techniques. The intensive development of scientific apparatus and methods in light spectroscopy leads to build various airborne or underwater specialized devices (UV-, IR-scanners, fluorosensors), which allows to detect oil pollution in relatively large areas of the sea. The possibility of measurements of oil pollution in the seawater is particularly important for monitoring, especially in very difficult weather conditions and highly waved sea surface. Such possibilities should be given by devices for in-situ measurements based on time-resolved fluorescence. Such measurements allow obtaining the significant information about presence of oil in really short time. As we show in this study, the wide-range development of fluorescence techniques opens the diagnostic opportunity not only for detection of the oil-in-water content but also for identification the type of oil directly in seawater or even determination of the source of oil pollution.

Źródło:

Journal of KONES; 2012, 19, 1; 15-20
1231-4005
2354-0133

Pojawia się w:

Journal of KONES

Dostawca treści:

Biblioteka Nauki

/5

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Skocz do pozycji: 3.

Tytuł:

O nieznanej naturze meteorytów
On the unknown nature of meteorites

Autorzy:

Kosina, Romuald

Powiązania:

https://bibliotekanauki.pl/articles/1033006.pdf

Data publikacji:

2014

Wydawca:

Polskie Towarzystwo Meteorytowe

Tematy:

UV fluorescence
meteorites
variation

Opis:

The variable UV-fluorescence of minerals is well known, and this is attributed to some defects in crystallographic structure or some admixtures, e.g. REE elements. So far, UV-fluorescence has not been applied to describe the nature of meteorites. The plates of forty-five meteorites were examined in three simultaneous emission spectra under an epi-fluorescence microscope. Meteorites were characterized quantitatively according to twelve colours of fluorescence, arbitrarily identified. A numerical method of non-metric multidimensional scaling was used to put colour characteristics as well as the meteorites into an ordination space. Each colour characteristic carries its own information independent of the others. NWA 4039, Bilanga, Gujba and NWA 5437 are extremes in the ordination space. Sahara 560 and NWA 791 expressed very poor and very rich fluorescence, respectively. UV-fluorescence can be a good screening method to quickly distinguish space minerals with crystallographic defects and/or those which are contaminated by REE elements. In subsequent research, other more advanced methods will have to be used.

Źródło:

Acta Societatis Metheoriticae Polonorum; 2014, 5; 72-80
2080-5497

Pojawia się w:

Acta Societatis Metheoriticae Polonorum

Dostawca treści:

Biblioteka Nauki

/5

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Skocz do pozycji: 4.

Tytuł:

In vitro fluorescence studies of transcription factor IIB-DNA interaction

Autorzy:

Górecki, Andrzej
Figiel, Małgorzata
Dziedzicka-Wasylewska, Marta

Powiązania:

https://bibliotekanauki.pl/articles/1038975.pdf

Data publikacji:

2015

Wydawca:

Polskie Towarzystwo Biochemiczne

Tematy:

transcription factors
TF2B
TFIIB
fluorescence spectroscopy
fluorescence quenching
site directed mutagenesis

Opis:

General transcription factor TFIIB is one of the basal constituents of the preinitiation complex of eukaryotic RNA polymerase II, acting as a bridge between the preinitiation complex and the polymerase, and binding promoter DNA in an asymmetric manner, thereby defining the direction of the transcription. Methods of fluorescence spectroscopy together with circular dichroism spectroscopy were used to observe conformational changes in the structure of recombinant human TFIIB after binding to specific DNA sequence. To facilitate the exploration of the structural changes, several site-directed mutations have been introduced altering the fluorescence properties of the protein. Our observations showed that binding of specific DNA sequences changed the protein structure and dynamics, and TFIIB may exist in two conformational states, which can be described by a different microenvironment of W52. Fluorescence studies using both intrinsic and exogenous fluorophores showed that these changes significantly depended on the recognition sequence and concerned various regions of the protein, including those interacting with other transcription factors and RNA polymerase II. DNA binding can cause rearrangements in regions of proteins interacting with the polymerase in a manner dependent on the recognized sequences, and therefore, influence the gene expression.

Źródło:

Acta Biochimica Polonica; 2015, 62, 3; 413-421
0001-527X

Pojawia się w:

Acta Biochimica Polonica

Dostawca treści:

Biblioteka Nauki

/5

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Skocz do pozycji: 5.

Tytuł:

A coumarin-based fluorescent probe for specific and rapid detection of fluoride ions

Autorzy:

Xin, Z.-H.
Meng, Y.-L.
Wu, Y.-H.
Feng, Z.-K.
Kang, Y.-F.

Powiązania:

https://bibliotekanauki.pl/articles/778907.pdf

Data publikacji:

2018

Wydawca:

Zachodniopomorski Uniwersytet Technologiczny w Szczecinie. Wydawnictwo Uczelniane ZUT w Szczecinie

Tematy:

Fluorion
Fluorescence
Coumarin
Probe
Bioimaging

Opis:

A simple and coumarin-based fluorescence probe has been designed and synthesized with silyl group as recognition group of fluoride ions (F−) in this study. The results showed that the fluorescence intensity of the probe displayed prominent enhancement with addition of F− at 445 nm with incubation of 1 min. There was an excellent linear relationship between fluorescence intensity and fluoride concentration from 0 to 30 μM (0~0.57 ppm), which offered the important condition for the quantitative analysis. In addition, the highly selective response to fluorion, the low detection limit with 28 nM (0.532 ppb), low toxicity and bioimaging afforded an advantage for practical application and detecting fluoride in biological systerms.

Źródło:

Polish Journal of Chemical Technology; 2018, 20, 4; 1-5
1509-8117
1899-4741

Pojawia się w:

Polish Journal of Chemical Technology

Dostawca treści:

Biblioteka Nauki

/5

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Skocz do pozycji: 6.

Tytuł:

Future prospects for investigating ciliate biodiversity

Autorzy:

Rajter, Ľubomír
Lu, Borong
Rassoshanska, Erika
Dunthorn, Micah

Powiązania:

https://bibliotekanauki.pl/articles/52000785.pdf

Data publikacji:

2022

Wydawca:

Uniwersytet Jagielloński. Wydawnictwo Uniwersytetu Jagiellońskiego

Tematy:

Biodiversity
Ciliophora
fluorescence
staining methods

Opis:

Ciliates have a long history of being central in evolutionary and ecological studies on eukaryotic microorganisms. Although thousands of species have been discovered, their total diversity still remains unknown. Here, we will discuss two unsolved problems that hinder the further exploration of ciliate diversity at the species level, and potential solutions to these problems are proposed. First, ciliate morphospecies are difficult to identify because the different silver stains are not scalable (they do not represent high-throughput methods) and basic supplies are lacking (e.g., protargol); a solution may be the development of fluorescent staining techniques. Second, ciliate phylogenetic species are difficult to identify because of extensive paralogy in nuclear-protein-coding genes; a solution may be to concentrate on sequencing mitochondrial genomes. These two approaches could be integrated into a high-throughput fluorescent-single-cell sorting and mitochondrial genomes sequencing process that would enable the observation and better understanding of ciliate species on a massive scale.

Źródło:

Acta Protozoologica; 2022, 61; 35-46
0065-1583
1689-0027

Pojawia się w:

Acta Protozoologica

Dostawca treści:

Biblioteka Nauki

/5

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Skocz do pozycji: 7.

Tytuł:

Obrazowanie struktury chondrytu Kuźnica w mikroskopie konfokalnym
Imaging the structure of the Kuźnica chondrite with a confocal microscope

Autorzy:

Paterczyk, Bohdan
Woźniak, Marek

Powiązania:

https://bibliotekanauki.pl/articles/30146481.pdf

Data publikacji:

2024-04

Wydawca:

Polskie Towarzystwo Meteorytowe

Tematy:

meteorite
Kuźnica
confocal microscopy
fluorescence

Opis:

Confocal microscopy is commonly used in mineralogy for structural analysis and to investigate the chemical composition of minerals. In our work we performed imaging of structural defects in meteorites i.e. imaging of microstructures, inclusions, fractures and cracks. Information obtained in this way enables better understanding of minerals' origin and evolution. The aim of our work was not concentrated on the deep description of physical processes related to confocal microscope (CSLM) imaging. This paper is limited to the presentation of a few CSLM functions and possibilities, which have practical application in the interpretation of fluorescence microscope imaging of minerals. Confocal microscopy enables identification and preview of localization of selected minerals and therefore can be used to image of the distribution of chemical compounds in minerals.

Źródło:

Acta Societatis Metheoriticae Polonorum; 2024, 15; 96-115
2080-5497

Pojawia się w:

Acta Societatis Metheoriticae Polonorum

Dostawca treści:

Biblioteka Nauki

/5

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Skocz do pozycji: 8.

Tytuł:

Antiproliferative effect of β-escin - an in vitro study

Autorzy:

Mojžišová, Gabriela
Kello, Martin
Pilátová, Martina
Tomečková, Vladimíra
Vašková, Janka
Vaško, Ladislav
Bernátová, Silvia
Mirossay, Ladislav
Mojžiš, Ján

Powiązania:

https://bibliotekanauki.pl/articles/1038845.pdf

Data publikacji:

2016

Wydawca:

Polskie Towarzystwo Biochemiczne

Tematy:

escin
apoptosis
mitochondria
fluorescence fingerprint

Opis:

This study examined the antiproliferative effects of β-escin (E) in cancer cells. The study showed that E inhibited cancer cells growth in a dose-dependent manner. The flow cytometric analysis revealed an escin-induced increase in the sub-G1 DNA content, which is considered to be a marker of apoptosis. Apoptosis was also confirmed by annexin V staining and DNA fragmentation assay. These effects were associated with increased generation of reactive oxygen species (ROS), caspase-3 activation and decreased mitochondrial membrane potential (MMP). Moreover, escin decreased mitochondrial protein content and mitochondrial fluorescence intensity as well as caused depletion of glutathione (GSH). However, activity of glutathione peroxidase (GPx) and glutathione reductase (GR) was not significantly changed in escin-treated cells. In conclusion, our results demonstrated that E has apoptotic effects in human cancer cells through the mechanisms involving mitochondrial perturbation. Although the exact mechanism needs to be investigated further, it can be concluded that E may be a useful candidate agent for cancer treatment.

Źródło:

Acta Biochimica Polonica; 2016, 63, 1; 79-87
0001-527X

Pojawia się w:

Acta Biochimica Polonica

Dostawca treści:

Biblioteka Nauki

/5

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Skocz do pozycji: 9.

Tytuł:

Zastosowanie techniki stroboskopowej do pomiarów czasów życia fluorescencji materiałów biologicznych. Cz. 1, Podstawy metody, aparatura i metodyka pomiarów
Application of stroboscopic technique for measurement of biological materials fluorescence lifetimes. Pt. 1, Basics of stroboscopic technique, instruments and methods

Autorzy:

Włodarski, M.
Kwaśny, M.
Kaliszewski, M.
Rutecka, B.

Powiązania:

https://bibliotekanauki.pl/articles/209313.pdf

Data publikacji:

2009

Wydawca:

Wojskowa Akademia Techniczna im. Jarosława Dąbrowskiego

Tematy:

czas życia fluorescencji
metoda stroboskopowa
fluorescencja białek
UV LED
fluorescence lifetime
stroboscopic method
protein fluorescence

Opis:

W pracy przedstawiono podstawy stroboskopowej metody pomiarów czasów życia fluorescencji, aparaturę, opracowaną metodykę pomiarów i analizę procesu dekonwolucji sygnałów. Pokazano, że krzywe zaniku fluorescencji z wykorzystaniem źródeł światła typu LED UV, (280 i 340 nm) mogą znaleźć zastosowanie do charakterystyki aminokwasów, białek, bakterii - w tym symulantów i interferentów bojowych środków biologicznych. Podstawowymi zaletami metody są: jej szybkość, możliwość wykorzystania tanich źródeł wzbudzenia, wysoka czułość, prostota obsługi, modułowa budowa umożliwiająca szybką wymianę źródeł wzbudzenia. Badania przeprowadzone na standardowych wzorcach - NATA, NADH wykazały zgodność czasów życia fluorescencji z danymi literaturowymi.
Stroboscopic method for determination of fluorescence lifetimes, using 280 nm and 340 nm UV LED sources, may be used for characterization of biological substances - including simulants and interferents of Biological Warfare Agents (BWA). Main advantages of this method are: quickness of measurement, cheap UV sources, high sensitivity, simplicity of operation, modular construction allowing quick changing of sources. Principle of stroboscopic method, developed method of measurements and analysis of deconvolution process are presented in this work. Results of measurement of NATA and NADH standards correspond to data published by other authors.

Źródło:

Biuletyn Wojskowej Akademii Technicznej; 2009, 58, 4; 165-176
1234-5865

Pojawia się w:

Biuletyn Wojskowej Akademii Technicznej

Dostawca treści:

Biblioteka Nauki

/5

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Skocz do pozycji: 10.

Tytuł:

Synthesis and Characterization of an Efficient New Liquid Laser Dye Material - Chalcone (DMAPPP)

Autorzy:

Elzupir, A.
Ibnaouf, K.
Idriss, H.
Ibrahem, M.
Prasad, S.
Alrajhi, M.
AlSalhi, M.
Alaamer, A.

Powiązania:

https://bibliotekanauki.pl/articles/1030569.pdf

Data publikacji:

2018-01

Wydawca:

Polska Akademia Nauk. Instytut Fizyki PAN

Tematy:

chalcone
DMAPPP
solvents effect
fluorescence
ASE

Opis:

This paper comprises the synthesis and characterization of 3-(4-(dimethylamino)phenyl)-1-phenyl-(2E)-propen-1-one (DMAPPP) and its application as a new laser medium. The absorption and fluorescence spectra of DMAPPP under different solvents and concentrations have been investigated. The amplified spontaneous emission performance of DMAPPP under various concentrations, organic solvents and pump pulse energies of Nd:YAG laser (355 nm) was also studied. The amplified spontaneous emission spectra of DMAPPP in solution were compared with a conventional laser dye of coumarin 503, under the same identical conditions. The gain and the fluorescence quantum yield of DMAPPP were determined. The most important features are: (1) DMAPPP has an excellent photochemical stability, (2) the amplified spontaneous emission from the DMAPPP was tuned in the wavelength region between 515 and 548 nm. This could be the first detailed paper on laser properties of DMAPPP.

Źródło:

Acta Physica Polonica A; 2018, 133, 1; 121-125
0587-4246
1898-794X

Pojawia się w:

Acta Physica Polonica A

Dostawca treści:

Biblioteka Nauki

/5

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Skocz do pozycji: 11.

Tytuł:

Aktywowana termicznie opóźniona fluorescencja (TADF) – jako rozwiązanie problemu niskich wydajności układów fluorescencyjnych OLED
Thermally activated delayed fluorescence – as a solution to the low yield problem of the fluorescent OLEDs

Autorzy:

Pander, P.

Powiązania:

https://bibliotekanauki.pl/articles/1215145.pdf

Data publikacji:

2016

Wydawca:

Stowarzyszenie Inżynierów i Techników Przemysłu Chemicznego. Zakład Wydawniczy CHEMPRESS-SITPChem

Tematy:

OLED
TADF
opóźniona fluorescencja
delayed fluorescence

Opis:

Rozwój technologii wyświetlania obrazu doprowadził do opracowania ekranów typu OLED, których użycie jest coraz powszechne. Współcześnie dąży się do poprawy kwantowej wydajności (EQE) OLED. Organiczne diody elektroluminescencyjne zawsze cierpiały z powodu ograniczonej wydajności kwantowej, co jest wywołane zdolnością wykorzystania zaledwie 25% stanów wzbudzonych. TADF, czyli aktywowana termicznie opóźniona fluorescencja, umożliwia użycie do 100% stanów wzbudzonych powstających w diodzie, a zatem zmniejszeniu ulega udział bezemisyjnej relaksacji cząsteczek. Zjawisko to występuje dla cząsteczek o małej całce nakładania HOMO i LUMO, co skutkuje małą przerwą energetyczną singlet-tryplet.
The development of display technology led to invention of OLED screens whose use is becoming more common nowadays. Today one of the goals is to improve OLED external quantum efficiency (EQE). Organic light-emitting diodes have always suffered from limited quantum efficiency due to harvesting of only 25% of excited states. TADF, which is thermally activated delayed fluorescence, allows to harvest up to 100% of excited states formed in the OLED, thus to decrease a contribution of the non-radiative relaxation. This phenomenon occurs for molecules possessing a small integral overlap of HOMO and LUMO, which results in a small singlet-triplet energy gap.

Źródło:

Chemik; 2016, 70, 6; 318-325
0009-2886

Pojawia się w:

Chemik

Dostawca treści:

Biblioteka Nauki

/5

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Skocz do pozycji: 12.

Tytuł:

Predicting emission spectra of fluorescent materials from their absorbance spectra using the artificial neural network

Autorzy:

Shams-Nateri, A
Piri, N

Powiązania:

https://bibliotekanauki.pl/articles/173176.pdf

Data publikacji:

2015

Wydawca:

Politechnika Wrocławska. Oficyna Wydawnicza Politechniki Wrocławskiej

Tematy:

fluorescence
prediction
absorbance
emission
neural networks

Opis:

Artificial neural networks have been shown to be able to approximate any continuous nonlinear functions and have been used to build data based empirical models for nonlinear processes. This work studies primarily the performance of neural networks as a tool for predicting the emission spectra of fluorescent materials from their absorbance, and further, tends to the determination of the optimal topology of the neural network for this purpose. In order to do this, spectral data were initially analyzed by a principal component analysis technique. The first four principal components were used as input nodes of neural networks with various training algorithms – namely cascade- and feed-forward algorithms – and also, various numbers of hidden layers and nodes. The obtained results indicate that the RMS error in a testing data set decreased with increasing the number of neurons and the minimal network architecture for a data prediction problem consists of two hidden layers, respectively with 9 and 1 nodes for both neural networks. Additionally, a better performance was obtained with the cascade-forward neural network, especially in a small number of nodes. The obtained results indicate that the neural networks can be used to provide a relationship between the absorbance as an input and the emission as a target.

Źródło:

Optica Applicata; 2015, 45, 4; 545-557
0078-5466
1899-7015

Pojawia się w:

Optica Applicata

Dostawca treści:

Biblioteka Nauki

/5

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Skocz do pozycji: 13.

Tytuł:

Uptake of photofrin II, a photosensitizer used in photodynamic therapy, by tumour cells in vitro

Autorzy:

Chwiłkowska, Agnieszka
Saczko, Jolanta
Modrzycka, Teresa
Marcinkowska, Anna
Malarska, Anna
Bielewicz, Julita
Patalas, Dorota
Banaś, Teresa

Powiązania:

https://bibliotekanauki.pl/articles/1043629.pdf

Data publikacji:

2003

Wydawca:

Polskie Towarzystwo Biochemiczne

Tematy:

photofrin II
photodynamic therapy
fluorescence spectra

Opis:

Photosensitizing dyes are used in fluorescence diagnostics and photodynamic therapy (PDT). These usually hematoporphyrin derivatives (HpD) accumulate preferentially within neoplastic tissues. HpD is a mixture of ether and ester linked porphyrins. Its partially purified form is known under the commercial name of photofrin II (PII). PII emission spectra were studied in a hydrophilic (PBS) and a lipophilic (PC liposomes) environment. Red shift was observed in their emission maxima from 615 nm in buffer solution to 635 nm in lipid. Identical red shift was obtained when the intracellular fluorescence of two cancer cell lines, MCF 7 and Jurkat, incubated with PII was investigated. Thus, intramembrane localization of PII may be suggested. As determined from the total fluorescence intensity, the uptake of PII was only slightly higher for Jurkat than for MCF 7 cells. Nevertheless the kinetics of the uptake was found to be different for both cell lines.

Źródło:

Acta Biochimica Polonica; 2003, 50, 2; 509-513
0001-527X

Pojawia się w:

Acta Biochimica Polonica

Dostawca treści:

Biblioteka Nauki

/5

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Skocz do pozycji: 14.

Tytuł:

The phenomenon of fluorescence in immunosensors

Autorzy:

Kłos-Witkowska, Aleksandra

Powiązania:

https://bibliotekanauki.pl/articles/1038798.pdf

Data publikacji:

2016

Wydawca:

Polskie Towarzystwo Biochemiczne

Tematy:

biosensor
immunosensor
fluorescence
absorbance
UV/Vis

Opis:

The phenomenon of fluorescence in immunosensors is described in this paper. Both structure and characteristics of biosensors and immunosensors are presented. Types of immunosensors and the response of bioreceptor layers to the reaction with analytes as well as measurements of electrochemical, piezoelectric and optical parameters in immunosensors are also presented. In addition, detection techniques used in studies of optical immunosensors based on light-matter interactions (absorbance, reflectance, dispersion, emission) such as: UV/VIS spectroscopy, reflectometric interference spectroscopy (RIfs), surface plasmon resonance (SPR), optical waveguide light-mode spectroscopy (OWLS), fluorescence spectroscopy. The phenomenon of fluorescence in immunosensors and standard configurations of immunoreactions between an antigen and an antibody (direct, competitive, sandwich, displacement) is described. Fluorescence parameters taken into account in analyses and fluorescence detection techniques used in research of immunosensors are presented. Examples of immunosensor applications are given.

Źródło:

Acta Biochimica Polonica; 2016, 63, 2; 215-221
0001-527X

Pojawia się w:

Acta Biochimica Polonica

Dostawca treści:

Biblioteka Nauki

/5

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Skocz do pozycji: 15.

Tytuł:

Spectroscopic Methods Studies in Polymer

Autorzy:

Rahi, Saad Khalid
Hassan, Ehssan Salah
Abd, Ahmed N.

Powiązania:

https://bibliotekanauki.pl/articles/1190136.pdf

Data publikacji:

2016

Wydawca:

Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN

Tematy:

ESCA
polymer
Fluorescence spectra
Excitation wavelength

Opis:

The physico – chemical changes induced in polymers following to radiation can be studied by a range of spectroscopic techniques. Recent developments in instrumentation and data analysis procedures in electronic, vibrational and magnetic resonance spectroscopes have provided considerable new insights, into polymer structure and behavior. The applications of these spectroscopic methods in polymer studies are reviewed with emphasis on their utility in investigations of radiation effects on macromolecules

Źródło:

World Scientific News; 2016, 42; 41-53
2392-2192

Pojawia się w:

World Scientific News

Dostawca treści:

Biblioteka Nauki

/5

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Skocz do pozycji: 16.

Tytuł:

Chemical characterization of PM10 in two small towns located in South Poland

Autorzy:

Turek-Fijak, Anna
Brania, Joanna
Styszko, Katarzyna
Zięba, Damian
Stęgowski, Zdzisław
Samek, Lucyna

Powiązania:

https://bibliotekanauki.pl/articles/1841646.pdf

Data publikacji:

2021

Wydawca:

Instytut Chemii i Techniki Jądrowej

Tematy:

air pollution
EDXFR fluorescence
PM10

Opis:

The purpose of this study is to analyse the elements and PM10 concentrations in air samples gathered in the winter of 2017/2018 in two small towns, namely Skala and Wadowice. The chemical elements were identified for each sample using the energy dispersive X-ray method. The spectrometer was equipped, among others, with an Mo-X-ray tube which was the source of the photons and the Si(Li) detector. The following chemical elements: Cl, K, Fe, Ca, Zn, Pb, Br, Ti, Cu, Mn, V, Co, Rb, Ni, Sr, and Cr were identifi ed in the samples. In addition, As and Se were identifi ed in Wadowice. First, the results were compared with each other and then with the results for the nearest city. It was observed that the PM10 concentrations were significantly higher than the UE limit value for PM10, which equals 50 gm−3 per 24 h. Moreover, the high concentrations of, among others, K, Pb, Cl or Zn, are likely to be linked with fossil fuels combustion and biomass burning. The levels of element concentrations in Wadowice and Skala resemble the levels observed several years earlier in Krakow.

Źródło:

Nukleonika; 2021, 66, 1; 29-34
0029-5922
1508-5791

Pojawia się w:

Nukleonika

Dostawca treści:

Biblioteka Nauki

/5

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Skocz do pozycji: 17.

Tytuł:

Spectrofluorometric characterization of oil from pyrolysis of scrap tires

Autorzy:

Ambrosewicz-Walacik, M.
Piętak, A.

Powiązania:

https://bibliotekanauki.pl/articles/950184.pdf

Data publikacji:

2016

Wydawca:

Instytut Techniczny Wojsk Lotniczych

Tematy:

fuels
used car tires
pyrolysis
fluorescence

Opis:

Almost all kinds of fuel applied in combustion engines come from refining industry, in which crude oil serves as basic raw material. However, there are also searched other sources of hydrocarbons which can be used directly or as additives to conventional fuels. The most popular in this regard are vegetable oils such as rapeseed oil, from which are produced esters used as fuel for diesel engines. On the other hand, as additives to gasoline can be used alcohols derived from the fermentation of agricultural products. Another way to obtain liquid hydrocarbons is pyrolysis of scrap tires. In this article, such pyrolytic product is analysed in terms of its fluorescence properties compared to the fluorescent properties of substances derived from the distillation of the pyrolysis product in three following temperatures: 160, 204 and 350 ºC. All the tested substances show relatively strong fluorescence in ultraviolet range. Fluorometric spectra were performed applying the spectrofluorometer Hitachi F-7000 FL with 1x1 cm quartz cuvette. Studied substances were previously diluted in n-hexane to obtain concentration 4 ppm. Amount of fluorescent peaks and their positions depends on kind of distillate. Ipso facto fluorescence method seems be suitable much more – than chemical analyse – for quick identification of substances constituting a components of distillate obtained from pyrolytic product.

Źródło:

Journal of KONES; 2016, 23, 1; 25-30
1231-4005
2354-0133

Pojawia się w:

Journal of KONES

Dostawca treści:

Biblioteka Nauki

/5

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Skocz do pozycji: 18.

Tytuł:

Aqueous Dispersion and Temperature Induced Reversible Fluorescence Properties of 1-Pyrenecarboxaldehyde

Autorzy:

Ashaduzzaman, Md.
Kunitake, M.

Powiązania:

https://bibliotekanauki.pl/articles/411942.pdf

Data publikacji:

2013

Wydawca:

Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN

Tematy:

monomeric distribution
1-pyrenecarboxaldehyde
reversible fluorescence

Opis:

The fluorescence intensities for various vibronic fine structures in the 1-pyrenecarboxaldehyde (PyCHO) fluorescence show strong environment dependence. In aqueous solvent, the distribution of dye is highly depended on its concentration labels and varies from excimer to monomeric state. UVVis Spectroscopic analysis could not able to detect dye below 10-7M concentration, whereas a New monomeric peak at 342 nm was observed after heating and cooling treatment at above the concentration. At 10-8M concentration, only monomeric distribution of 1-PyCHO reveals a strong temperature (20-50 ?C) induced reversible perturbation of the vibronic band intensities. This suggests the operation of some specific solute-solvent dipole-dipole interaction mechanism strongly influence by heating.

Źródło:

International Letters of Chemistry, Physics and Astronomy; 2013, 1; 55-62
2299-3843

Pojawia się w:

International Letters of Chemistry, Physics and Astronomy

Dostawca treści:

Biblioteka Nauki

/5

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Skocz do pozycji: 19.

Tytuł:

Biosensory proteinowe i ich właściwości fluorescencyjne
Protein based biosensors and their fluorescent properties

Autorzy:

Kłos-Witkowska, A.

Powiązania:

https://bibliotekanauki.pl/articles/157734.pdf

Data publikacji:

2014

Wydawca:

Stowarzyszenie Inżynierów i Techników Mechaników Polskich

Tematy:

biosensor
fluorescencja
proteina
analit
fluorescence
protein

Opis:

W pracy zostały opisane biosensory proteinowe oraz ich właściwości fluorescencyjne. Przybliżono podstawy projektowania biosensorów proteinowych oraz strategii stosowanych przy projektowaniu sensorów białkowych:rational protein design (racjonalne projektowanie), directed evolution (ewolucja ukierunkowana), de novo design (projektowanie białek de novo).Zestawiono anality oraz proteiny receptorowe wykorzystywane w badaniach fluorescencyjnych. Opisano zastosowania biosensorów w medycynie.
This paper describes the basics of protein biosensors with particular emphasis on their fluorescent properties. Biosensors are classified on receptor layers with particular emphasis on protein biosensors. There is brought closer the base of protein sensor design, taking into account three strategies commonly use in protein engineering in order to improve sensivity, selectivity and stability of protein biosensor: rational design, directed evolution, de novo design. The main construction problems encountered in the design of biosensor and ways of they solving have been described. The recent research on fluorescent protein biosensors receptor layer is presented. In Table 1 there is given the list of proteins and appropriate fluorescent methods: FRET (Föster Resonance energy Transfer), change in fluorescence emission spectra, for Zn2+, Glc ,Gal ,Fruc, Arab, DMAPIP-b, Ca2+, H2O2, Ca2+, β-CD,CB6, CB7, CFP detection. There is described the use of fluorescent protein based biosensors for clinical application. Biosensors as modern and widely used tools in environmental, medical, food industry have been presented and the future trends in scientific research have been given in the paper.

Źródło:

Pomiary Automatyka Kontrola; 2014, R. 60, nr 6, 6; 378-381
0032-4140

Pojawia się w:

Pomiary Automatyka Kontrola

Dostawca treści:

Biblioteka Nauki

/5

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Skocz do pozycji: 20.

Tytuł:

Analizatory DNA/PCR typu lab-chip z detekcją fluorymetryczną
Lab-on-a-chip DNA/PCR analyzers with fluorometric detection

Autorzy:

Walczak, R.

Powiązania:

https://bibliotekanauki.pl/articles/154783.pdf

Data publikacji:

2010

Wydawca:

Stowarzyszenie Inżynierów i Techników Mechaników Polskich

Tematy:

real-time PCR
lab-chip
fluorescencja
fluorescence

Opis:

W artykule przedstawiono przegląd analizatorów DNA wykorzystujących technikę real-time PCR i lab-chipy. Zaprezentowano opis opracowanej metodologii i instrumentarium do detekcji fluorescencji z wykorzystaniem miniaturowych komponentów optoelektronicznych i "inteligentnego" oprogramowania analizującego. Przedstawiono dwa przykłady miniaturowych analizatorów real-time PCR/DNA opracowanych w ramach projektów europejskich i krajowych wykorzystujących lab-chipy i nowatorską metodę detekcji fluorymetrycznej.
The paper presents a novel miniaturized optical instrumentation for fluorescence excitation and detection for miniaturized real-time PCR analyzers using lab-on-a-chip (LOC) devices. Application of miniaturized semiconductor laser to fluorescence excitation, CCD-minicamera as fluorescence photodetector and specialized software for optical signal conditioning led to development of low-cost and highly sensitive optical instrumentation. To carry out full characterization of the optical instrumentation, miniaturized thermocycler co-working with LOC was built. The optical instrumentation was tested with three LOC made of different materials and technologies, giving proper detection of fluorescence signals during real-time PCR. Finally, two miniaturized devices for real-time PCR detection and identification of DNA and utilizing described here novel optical instrumentation were briefly described. The scheme and technical realization of the novel instrumentation in laboratory version is shown in Fig. 4. The detection limit of DNA was about 0,1 ng/ml (Fig. 5). It was also found that the optical instrumentation co-works with different constructions of lab-on-a-chips for DNA real-time PCR amplification (Fig. 6). The developed optical instrumentation was successfully applied to two miniaturized devices for DNA detection and identification by real-time PCR. Technical realizations of the devices and views of the lab-on-a-chips are shown in Figs. 7 and 9. In both cases, proper detection of fluorescence signals generated during real-time PCR of Campylobacter j. DNA (Fig. 8) or complementary DNA (Fig. 10) were observed. It confirmed high sensitivity of the developed optical instrumentation.

Źródło:

Pomiary Automatyka Kontrola; 2010, R. 56, nr 7, 7; 805-808
0032-4140

Pojawia się w:

Pomiary Automatyka Kontrola

Dostawca treści:

Biblioteka Nauki

/5

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Skocz do pozycji: 21.

Tytuł:

Quantitative analysis of the ternary complex of RNA polymerase, cyclic AMP receptor protein and DNA by fluorescence anisotropy measurements

Autorzy:

Bonarek, Piotr
Kędracka-Krok, Sylwia
Kępys, Barbara
Wasylewski, Zygmunt

Powiązania:

https://bibliotekanauki.pl/articles/1040712.pdf

Data publikacji:

2008

Wydawca:

Polskie Towarzystwo Biochemiczne

Tematy:

cAMP receptor protein
fluorescence anisotropy
RNA polymerase

Opis:

The in vitro formation of transcription complexes with Escherichia coli RNA polymerase was monitored using fluorescence anisotropy measurements of labeled fragments of DNA. The multicomponent system consisted of holo or core RNA polymerase (RNAP) and lac or gal promoter fragments of DNA (in different configurations), in the presence or absence of CRP activator protein (wt or mutants) with its ligand, cAMP. Values of the apparent binding constants characterizing the system were obtained, as a result of all processes taking place in the system. The interaction of the promoters with core RNAP in the absence of CRP protein was characterized by apparent binding constants of 0.67 and 1.9 × 106 M-1 for lac166 and gal178, respectively, and could be regarded as nonspecific. The presence of wt CRP enhanced the strength of the interaction of core RNAP with the promoter, and even in the case of gal promoter it made this interaction specific (apparent binding constant 2.93 × 107 M-1). Holo RNAP bound the promoters significantly more strongly than core RNAP did (apparent binding constants 1.46 and 40.14 × 106 M-1 for lac166 and gal178, respectively), and the presence of CRP also enhanced the strength of these interactions. The mutation in activator region 1 of CRP did not cause any significant disturbances in the holo RNAP-lac promoter interaction, but mutation in activator region 2 of the activator protein substantially weakened the RNAP-gal promoter interaction.

Źródło:

Acta Biochimica Polonica; 2008, 55, 3; 537-547
0001-527X

Pojawia się w:

Acta Biochimica Polonica

Dostawca treści:

Biblioteka Nauki

/5

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Skocz do pozycji: 22.

Tytuł:

Allelopathic activity of the Synechococcus sp. (Cyanobacteria, Chroococcales) on selected cyanobacteria species

Autorzy:

Śliwińska-Wilczewska, Sylwia
Gergella, Kinga
Latała, Adam

Powiązania:

https://bibliotekanauki.pl/articles/2182150.pdf

Data publikacji:

2016-12-31

Wydawca:

Uniwersytet Pedagogiczny im. Komisji Edukacji Narodowej w Krakowie

Tematy:

allelopathy
cyanobacteria
fluorescence
growth
photosynthesis
photosynthetic pigments

Opis:

Picocyanobacterium Synechococcus sp. is very important but still poorly understood component of marine and freshwater ecosystems. In this study, the effect of single and multiple addition of cell-free filtrate obtained from Synechococcus sp. on selected cyanobacteria Synechocystis sp., Geitlerinema amphibium, Nodularia spumigena and Nostoc sp. was investigated. The species present in this work are groups of aquatic phototrophs known to co-occur in the Baltic Sea. The study showed that the picocyanobacterial cell-free filtrate inhibit the growth and changed the cell morphology of filamentous cyanobacteria G. amphibium, N. spumigena and Nostoc sp. It was shown that the addition of cell-free filtrate caused a decline of pigmentation and cell lysis of G. amphibium, N. spumigena and Nostoc sp. compared to the control culture. In addition, it was observed that the filtrate obtained from Synechococcus sp. did not affect the Synechocystis sp. It was found that the filtrate obtained from picocyanobacterium had the strongest effect on growth of G. amphibium, therefore for this cyanobacteria performed additional experiments to showed whether the filtrate affected also photosynthetic pigments, chlorophyll fluorescence and photosynthesis. The study proved that the picocyanobacterial allelopathic compounds reduce the efficiency of photosynthesis, which results in the inhibition of growth of target organisms. This way of interaction may explain the formation of almost monospecific cyanobacterial blooms in many aquatic ecosystems, including in the Baltic Sea.

Źródło:

Annales Universitatis Paedagogicae Cracoviensis Studia Naturae; 2016, 1; 115-126
2543-8832
2545-0999

Pojawia się w:

Annales Universitatis Paedagogicae Cracoviensis Studia Naturae

Dostawca treści:

Biblioteka Nauki

/5

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Skocz do pozycji: 23.

Tytuł:

A near-infrared fluorescent probe for selective detection of fluorion

Autorzy:

Kang, Yan-Fei
Wei, Fan
Meng, Ya-Li
Xin, Zhen-Hui
Wei, Dong

Powiązania:

https://bibliotekanauki.pl/articles/778367.pdf

Data publikacji:

2020

Wydawca:

Zachodniopomorski Uniwersytet Technologiczny w Szczecinie. Wydawnictwo Uczelniane ZUT w Szczecinie

Tematy:

Probe
Fluorescence
Fluoride anion
Selectivity
Near-infrared

Opis:

In this work, we have designed and synthesized the fluorescent probe 1, which was capable to selectively detect fluoride anion (F⁻). More importantly, the probe 1 possessed near-infrared excitation and emission wavelengths (excitation at 650 nm and emission at 695), and the probe solution had changed dramatically from yellow to cyan with the addition of F^–. In addition, the fluorescence intensity exhibited perfectly positive correlation with concentration of F⁻ concentration from 0 to 40 μM (R² = 0.9972), which offered the important condition for quantitative analysis. The probe 1 owned detection limit of 46 nM. Therefore, this near-infrared probe can be of great benefit for detecting F⁻ in practical application.

Źródło:

Polish Journal of Chemical Technology; 2020, 22, 1; 1-5
1509-8117
1899-4741

Pojawia się w:

Polish Journal of Chemical Technology

Dostawca treści:

Biblioteka Nauki

/5

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Skocz do pozycji: 24.

Tytuł:

Mobile phone as a fluorescence reader

Autorzy:

Shahin, H.
Walczak, R.

Powiązania:

https://bibliotekanauki.pl/articles/173637.pdf

Data publikacji:

2013

Wydawca:

Politechnika Wrocławska. Oficyna Wydawnicza Politechniki Wrocławskiej

Tematy:

mobile phone
fluorescence detection
laser
CMOS
cocaine

Opis:

A new achievement of mobile phone application for fluorometry to identify cocaine concentration using samples made of tested person sweat implementing special designed software has been presented in this paper. Accessibility is one of most dominant features of this method since the main important part is a mobile phone which can be found and used vastly. Reliability of data collected using this technique is strong enough to compete with past time consuming and expensive devices and equipment. The results enable to have a close and precise observation about the amount of cocaine concentration, the same as reference device outputs.

Źródło:

Optica Applicata; 2013, 43, 3; 413-420
0078-5466
1899-7015

Pojawia się w:

Optica Applicata

Dostawca treści:

Biblioteka Nauki

/5

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Skocz do pozycji: 25.

Tytuł:

Comparison of Methods in Studies of Cell Death Mechanisms

Autorzy:

Borkowska, A.
Nowakowski, M.
Miszczyk, J.
Lipiec, E.
Wiltowska-Zuber, J.
Rawojć, K.
Kwiatek, W.

Powiązania:

https://bibliotekanauki.pl/articles/1030322.pdf

Data publikacji:

2018-02

Wydawca:

Polska Akademia Nauk. Instytut Fizyki PAN

Tematy:

growth and division
cell processes
fluorescence
optimization

Opis:

While studying the influence of ionizing radiation or certain chemical agents on cells, it is crucial to not only determine cytotoxicity, but also to follow cell death mechanisms. There are different methods to screen processes of cell death and still very important question remains unanswered about differences in results that could be caused by various experimental steps in procedures. Based on literature review two protocols of cell death determination were compared. First protocol regarded collecting cells floating in medium before trypsinization and following centrifugation of them. In the second protocol floating cells were discarded and attached ones were stained and fixed. In all experiments three different untreated cell lines (A172, DU145 as cancer cell lines and in comparison, fibroblasts (FB CCL 110), as a non- cancerous cell line) were used to test applied protocols. Cells were cultured and death processes were examined at different time points up to 120 h. Compared protocols showed statistically significant differences, especially in terms of necrosis, which was higher when included floating cells from culture medium and then centrifuging them. Therefore, presented results show importance of choosing a valid experimental procedure in case of evaluating cells viability and types of cell death pathways quantitatively.

Źródło:

Acta Physica Polonica A; 2018, 133, 2; 263-266
0587-4246
1898-794X

Pojawia się w:

Acta Physica Polonica A

Dostawca treści:

Biblioteka Nauki

/5

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Skocz do pozycji: 26.

Tytuł:

Fluorescence decay time distribution analysis of cyclic enkephalin analogues; Influence of solvent and Leu configuration in position 5 on conformation

Autorzy:

Malicka, Joanna
Ganzynkowicz, Robert
Groth, Małgorzata
Czaplewski, Cezary
Karolczak, Jerzy
Liwo, Adam
Wiczk, Wiesław

Powiązania:

https://bibliotekanauki.pl/articles/1044169.pdf

Data publikacji:

2001

Wydawca:

Polskie Towarzystwo Biochemiczne

Tematy:

lifetime distribution
fluorescence
energy transfer
conformation
enkephalin

Opis:

Lifetime distribution analysis were performed to study the influence of Leu configuration in position 5 on changes of the peptide chain of cyclic analogues of enkephalins containing a fluorescence donor and acceptor in different solvents. The configuration change of Leu5 in all the analogues of enkephalins studied which contain donor-acceptor pairs has no apparent influence on Trp lifetime distributions. In contrast, there is a significant solvent effect on the shape of lifetime distribution.

Źródło:

Acta Biochimica Polonica; 2001, 48, 1; 95-102
0001-527X

Pojawia się w:

Acta Biochimica Polonica

Dostawca treści:

Biblioteka Nauki

/5

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Skocz do pozycji: 27.

Tytuł:

1;25 centric fusion recognition in the cattle chromosome by fluorescence in situ hybridization

Autorzy:

Ladon, D
Solinas-Toldo, S
Fries, R
Stranzinger, G F

Powiązania:

https://bibliotekanauki.pl/articles/2047680.pdf

Data publikacji:

1996

Wydawca:

Polska Akademia Nauk. Czytelnia Czasopism PAN

Tematy:

cattle chromosome
hybridization
fluorescence
cattle
centric fusion

Opis:

Fluorescence in situ hybridization (FISH) experiments with specific probes for chromosome 29 and 25 were carried out on a Brown Swiss bull, previously diagnosed as a carrier of 1;29 centric fusion. The hybridization of the chromosome 29-specific probe (BMC 4216 - already located on 29q13), produced signals on two small acrocentrics, but not on the translocated chromosome. The signals appeared on the translocated chromosome and on a single chromosome 25 after hybridization of the chromosome-specific probe (BMC 3224 - previously located on 25q24). According to the actual nomenclature, the analysed aberration is a robertsonian translocation involving chromosomes 1 and 25.

Źródło:

Journal of Applied Genetics; 1996, 37, 1; 101-104
1234-1983

Pojawia się w:

Journal of Applied Genetics

Dostawca treści:

Biblioteka Nauki

/5

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Skocz do pozycji: 28.

Tytuł:

Engine fuel characterisation based on excitation-emission spectra

Autorzy:

Baszanowska, E.
Otremba, Z.

Powiązania:

https://bibliotekanauki.pl/articles/246580.pdf

Data publikacji:

2017

Wydawca:

Instytut Techniczny Wojsk Lotniczych

Tematy:

fuel
diesel
engine
excitation-emission spectra
fluorescence

Opis:

Considering that correctly functioning of diesel ship engine depends on the composition of applied fuel, it is necessary to easy characterise fuels in terms of quality. Therefore taking into account above mentioned, appropriate analyses have been undertaken and the intention of this article is to inform that characterisation of fuel used in marine transport can be described by the specific parameter based on fluorescence excitation-emission spectroscopy. Therefore, for this study fuel typically used in ship engine – Diesel fuel – is considered. For analysis, Diesel fuel dissolved in n-hexane was used and several oil samples for various oil concentration were prepared. Spectrofluorometer Aqualog Horiba was used to record the excitation-emission spectra (EEMs). EEM spectra determined for each of oil concentration were described by two specific peaks for this kind of oil. Based on registered EEMs, the specific indicator - the wavelength-independent fluorescence maximum – coded in two specific wavelengths – excitation and emission, respectively, described as Exmax/Emmax was determined for considered oil concentration. Obtained results indicate the independence from oil concentration only for one detected peak described by Exmax/Emmax = 240/332. Taking into account obtained results, we can conclude that the wavelength-independent fluorescence maximum detected for excitation wavelength at 240 nm corresponds to the emission wavelength at 332 nm due to the independence from the oil concentration, could be a good indicator to characterise this kind of oil – Diesel fuel. We can conclude that the changes in the composition of fuel could be detected in EEM spectra by the change of detected peaks position.

Źródło:

Journal of KONES; 2017, 24, 2; 25-30
1231-4005
2354-0133

Pojawia się w:

Journal of KONES

Dostawca treści:

Biblioteka Nauki

/5

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Skocz do pozycji: 29.

Tytuł:

Application of a carbazole derivative as a spectroscopic fluorescent probe for real time monitoring of cationic photopolymerization

Autorzy:

Ortyl, J.
Galica, M.
Popielarz, R.
Bogdał, D.

Powiązania:

https://bibliotekanauki.pl/articles/778445.pdf

Data publikacji:

2014

Wydawca:

Zachodniopomorski Uniwersytet Technologiczny w Szczecinie. Wydawnictwo Uczelniane ZUT w Szczecinie

Tematy:

spectroscopic fluorescent probe
FPT
fluorescence
carbazole
photopolymerization

Opis:

The performance of 1-(9-ethylcarbazol-3-yl)-4,4,4-trifluorobutane-1,3-dione (1) as a fluorescent probe for the monitoring of cationic photopolymerization processes by Fluorescence Probe Technique (FPT) has been evaluated in comparison with the response of 7-diethylamino-4-methylcoumarin (Coumarin 1) (2). Triethylene glycol divinyl ether and diphenyliodonium hexafluorophosphate were used as an example monomer and a cationic photoinitiator respectively. It has been found that the probe 1 withstands the cationic polymerization conditions and provides correct probe response. 1-(9-ethylcarbazol-3-yl)-4,4,4-trifluorobutane-1,3-dione shifts its fluorescence spectrum with progress of cationic photopolymerization of the monomer, which enables the monitoring of the polymerization progress using the fluorescence intensity ratio measured at two different wavelengths as the progress indicator. By comparing the behavior of 1 and 2, it has been documented that the fluorescence spectrum of probe 1 shows a spectacular hypsochromic shift (Δλ = 33 nm) upon the monomer polymerization, while the shift of 2 is three times smaller (Δλ = 11 nm). Moreover, the sensitivity of probe 1 is more than 2.5-times higher than that of any other probes suitable for monitoring cationic polymerization processes, reported previously. Therefore, application of the carbazole derivative (1) as a new probe for the monitoring of the crosslinking process of coatings cured by cationic photopolymerization has been proposed.

Źródło:

Polish Journal of Chemical Technology; 2014, 16, 1; 75-80
1509-8117
1899-4741

Pojawia się w:

Polish Journal of Chemical Technology

Dostawca treści:

Biblioteka Nauki

/5

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Skocz do pozycji: 30.

Tytuł:

Adsorption of humic acid on mesoporous carbons prepared from poly- (ethylene terephthalate) templated with magnesium compounds

Autorzy:

Karolczyk, J.
Mozia, S.
Morawski, A.W.
Przepiórski, J.

Powiązania:

https://bibliotekanauki.pl/articles/778949.pdf

Data publikacji:

2012

Wydawca:

Zachodniopomorski Uniwersytet Technologiczny w Szczecinie. Wydawnictwo Uczelniane ZUT w Szczecinie

Tematy:

porous carbon
adsorption
humic acid
fluorescence spectroscopy

Opis:

Porous carbons obtained from poly(ethylene terephtalate) contained in a mixture with either MgCO3 or Mg(OH)2 were examined as adsorbents for removal of humic acid from water. Adsorption of the model contaminants is discussed in relation to the textural parameters of the obtained carbon materials. Pore structure parameters of the carbonaceous materials were strongly influenced by preparation conditions including temperature and relative amounts of the inorganics used during preparations as template. Porous carbons prepared revealed a potential to purify water from the model contaminant of high molecular weight. The results presented confirmed a key role of mesoporosity in the adsorption of humic acid. Fluorescence spectroscopy was confirmed to be an useful method to evaluate concentration of humic acid in water.

Źródło:

Polish Journal of Chemical Technology; 2012, 14, 4; 95-99
1509-8117
1899-4741

Pojawia się w:

Polish Journal of Chemical Technology

Dostawca treści:

Biblioteka Nauki

/5

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Skocz do pozycji: 31.

Tytuł:

Dansylated analogues of the opioid peptide [Dmt1]DALDA: in vitro activity profiles and fluorescence parameters.

Autorzy:

Berezowska, Irena
Lemieux, Carole
Chung, Nga
Zelent, Bogumil
Schiller, Peter

Powiązania:

https://bibliotekanauki.pl/articles/1043324.pdf

Data publikacji:

2004

Wydawca:

Polskie Towarzystwo Biochemiczne

Tematy:

fluorescence spectroscopy
opioid activity profile in vitro
fluorescence quantum yield
opioid peptides
fluorescent opioid peptide analogues
[Dmt1]DALDA

Opis:

Dansylated analogues of the potent and selective μ opioid peptide agonist [Dmt1]DALDA (H-Dmt-D-Arg-Phe-Lys-NH2; Dmt = 2',6'-dimethyltyrosine) were prepared either by substitution of Nβ-dansyl-α,β-diaminopropionic acid or Nε-dansyllysine for Lys4, or by attachment of a dansyl group to the C-terminal carboxamide function via a linker. All three analogues displayed high μ agonist potency in vitro and the C-terminally dansylated one retained significant μ receptor selectivity. The three analogues showed interesting differences in their fluorescence emission maxima and quantum yields, indicating that the dansyl group in two of them was engaged in intramolecular hydrophobic interactions. These dansylated [Dmt1]DALDA analogues represent valuable tools for binding studies, cellular uptake and intracellular distribution studies, and tissue distribution studies.

Źródło:

Acta Biochimica Polonica; 2004, 51, 1; 107-113
0001-527X

Pojawia się w:

Acta Biochimica Polonica

Dostawca treści:

Biblioteka Nauki

/5

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Skocz do pozycji: 32.

Tytuł:

The use of the fluorescence phenomenon to evaluate the content of feed ingredients
Wykorzystanie zjawiska fluorescencji do oceny zawartości składników pasz

Autorzy:

Matuszek, D.

Powiązania:

https://bibliotekanauki.pl/articles/336437.pdf

Data publikacji:

2017

Wydawca:

Sieć Badawcza Łukasiewicz - Przemysłowy Instytut Maszyn Rolniczych

Tematy:

feed
tracer
homogeneity
fluorescence
pasza
traser
homogeniczność
fluorescencja

Opis:

This paper presents the results of the evaluation of feed quality conducted on the basis of the content of a tracer coated with a fluorescent substance. The study used the example of compound feed mixture for laying hens. Feed ingredients: maize and kardi primed wet using fluorescent solutions. The substances, such as Tinopal, Uranine and Rhodamine B were excited to shining by ultraviolet radiation. Before mixing process started, two tracers coated with different fluorescent substances were introduced into the mixture. The acquired images of the samples were analysed using the Patan computer program and were the basis of the information about the percentage share of the specified key component. The tests were used to verify the premise that there is a possibility of simultaneous determination of several components in a mixture based on fluorescent method. The results confirmed this assumption.
W pracy przedstawiono wyniki oceny jakości paszy w oparciu o zawartość składnika kluczowego pokrytego substancją fluorescencyjną. Badania przeprowadzono na przykładzie wieloskładnikowej mieszanki paszowej dla kur niosek. Składniki paszy: kukurydze i kardi zaprawiano na mokro rozworami fluorescencyjnymi. Zastosowane substancje: tinopal, uranina i rodamina B wzbudzano do świecenie promieniowaniem ultrafioletowym. Pozyskane obrazy próbek poddawano analizie w programie komputerowym Patan, na podstawie którego uzyskiwano informacje o procentowej zawartości wskazanego składnika kluczowego. Do mieszanki przed rozpoczęciem procesu mieszania wprowadzano dwa trasery pokryte różnymi substancjami fluorescencyjnymi. Testy miały posłużyć do weryfikacji założenia, że istnieje możliwość jednoczesnego oznaczenia zawartości kilku składników w jednej mieszance w oparciu o metodę fluorescencyjną. Uzyskane wyniki potwierdziły założenie.

Źródło:

Journal of Research and Applications in Agricultural Engineering; 2017, 62, 2; 72-75
1642-686X
2719-423X

Pojawia się w:

Journal of Research and Applications in Agricultural Engineering

Dostawca treści:

Biblioteka Nauki

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Skocz do pozycji: 33.

Tytuł:

Optical properties of crude oil detected in seawater

Autorzy:

Haule, K.
Toczek, H.

Powiązania:

https://bibliotekanauki.pl/articles/245741.pdf

Data publikacji:

2014

Wydawca:

Instytut Techniczny Wojsk Lotniczych

Tematy:

crude oil
marine ecology
fluorescence
seawater
oil pollution

Opis:

The effects of crude oils entering marine environment have been investigated since early 1960s when oil pollution became a subject of global environmental concern. Extraction and transportation of crude oils have been contributing to water pollution especially in closed water basins, such as the Baltic Sea. It was estimated that yearly oil input to the Baltic Sea amounts to 21-70 thousands of tons, which is twice higher than in the North Sea and three times higher than in the North Atlantic. Growing pollution loads into the Baltic Sea created a demand for multifarious studies on the environmental effects of oil products. In this study, we focus on bio-optical and ecological aspects of the presence of crude oil in seawater. Dispersed oil droplets occur in seawater as the result of contaminated river inflows, bilge water discharges and as the consequence of mechanical and chemical dispersion of oil spills. Their optical properties depend on oil type, concentration and size distribution. We present further results obtained from the developed fluorescence-based method for determination of the crude oil concentration in natural Baltic seawater and for evaluation of the oil droplets size by applying vacuum filtering. The results have been measured for the samples collected in Southern Baltic Sea during several ship cruises in 2012. We discuss the application of vacuum filtering in the fluorescence analyses in the context of laboratory, in situ and remote detection of dispersed oil.

Źródło:

Journal of KONES; 2014, 21, 3; 127-132
1231-4005
2354-0133

Pojawia się w:

Journal of KONES

Dostawca treści:

Biblioteka Nauki

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Skocz do pozycji: 34.

Tytuł:

Fluorescence properties of mechanically dispersed crude oil

Autorzy:

Haule, K.
Toczek, H.

Powiązania:

https://bibliotekanauki.pl/articles/247844.pdf

Data publikacji:

2014

Wydawca:

Instytut Techniczny Wojsk Lotniczych

Tematy:

crude oil
marine ecology
fluorescence
seawater
oil pollution

Opis:

Despite of numerous actions undertook by the global and local authorities towards protection of marine environment, oil pollution loads into the Baltic Sea still tend to increase and need to be continuously estimated in order to apply the legal regulations. There is a demand for multifarious studies on the environmental effects of oil products. Dispersed oil droplets occur in seawater as the result of contaminated river inflows, bilge water discharges and as the consequence of mechanical and chemical dispersion of oil spills. Their optical properties depend on oil type, concentration and size distribution. Oil content influences many environmental factors, like water quality and bio-optical parameters (e.g. water-leaving radiance, inherent optical properties, seawater fluorescence). We present a unique study of the collected database of crude oil fluorescence spectra for better understanding the correlations between oil optical properties and its concentration, as well as for evaluation of the oil droplets size by the application of vacuum filtering using three filters of different permeability. Fluorescence spectra have been registered for oil-in-water emulsion samples prepared in the laboratory by mechanical dispersion. We discuss the optical properties of crude oil and the relationships between them in the context of potential remote detection of dispersed oil in seawater.

Źródło:

Journal of KONES; 2014, 21, 4; 161-167
1231-4005
2354-0133

Pojawia się w:

Journal of KONES

Dostawca treści:

Biblioteka Nauki

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Skocz do pozycji: 35.

Tytuł:

Laser based diagnostic system for spray measurements

Autorzy:

Kapusta, Ł. J.
Jaworski, P.
Teodorczyk, A.
Kowalski, J.

Powiązania:

https://bibliotekanauki.pl/articles/244911.pdf

Data publikacji:

2015

Wydawca:

Instytut Techniczny Wojsk Lotniczych

Tematy:

spray
shadowgraphy
Mie scattering
LIF
laser-induced fluorescence

Opis:

The paper discusses the capabilities of the laser diagnostic system for dispersed multiphase flows measurements, which was recently commissioned at the Institute of Heat Engineering (ITC) at Warsaw University of Technology. The system presented in the paper is multifunctional and is capable to use different measurements techniques such as shadowgraphy, Mie scattering (scattering on the gas-liquid interface) and LIF (laser induced fluorescence). It provides possibility of switching from one technique to another depending on the characteristics of the observed combustion/injection mixing process. The operational features are discussed in-detail. The study presents the required and possible system setups. Special attention was focused on the system components, which are needed for relevant measurement techniques. Moreover, the limitations of the measurement techniques are discussed and the example measurements are presented. The study shows that using different techniques extends the measurement capabilities of the system with minimum investment costs. This results from the fact that certain system components for different techniques are the same. Moreover, one can conclude that the system design allows certain measurement methods to be applied simultaneously, which results in synergy by making possible drawing more advanced conclusions on observed phenomena

Źródło:

Journal of KONES; 2015, 22, 3; 91-98
1231-4005
2354-0133

Pojawia się w:

Journal of KONES

Dostawca treści:

Biblioteka Nauki

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Skocz do pozycji: 36.

Tytuł:

Problematyka wyznaczenia precyzji metody oznaczania zawartości siarki w LPG według EN 17178:2019 w warunkach laboratorium INiG – PIB
The issue of determining the precision of the method of determining sulfur content in LPG according to EN 17178: 2019 in the conditions of the INiG – PIB laboratory

Autorzy:

Jędrychowska, Sylwia

Powiązania:

https://bibliotekanauki.pl/articles/1834101.pdf

Data publikacji:

2020

Wydawca:

Instytut Nafty i Gazu - Państwowy Instytut Badawczy

Tematy:

siarka
LPG
fluorescencja
UV
precyzja
sulphur
fluorescence
precision

Opis:

A new edition of the standard specification for liquefied hydrocarbon gas EN 589:2018 was introduced in 2018, which changes some parameters for this fuel, including the acceptable sulfur content. Also, this specification constitutes a new standard for determining volatile sulfur content in LPG, i.e. EN 17178: 2019. Studies were carried out to determine the precision of the method according to the above European standard. Repeatability was determined based on tests of 11 LPG samples with different sulfur content and next it was compared with the values given in the applicable standards: EN 17178: 2019 and ASTM D 6667-14 used until now. Each sample was analyzed 7 times. The repeatability values calculated according to EN 17178: 2019 are much lower than the repeatability according to ASTM D 6667-14 and the repeatability determined in this paper for samples with a concentration range above 4 mg/kg. The repeatability values determined in this work are lower than the values calculated according to EN 17178: 2019 and ASTM D 6667-14 for samples with concentrations below 4 mg/kg. The obtained results confirm the legitimacy of reservations of many European laboratories that perform tests of sulfur content in LPG and participants of the work of the CEN/TC 19/WG 23 N 231 working group, with regard of the correctness of determining the precision of the method pursuant to EN 17178: 2019. It was decided consequently to repeat interlaboratory studies that will be aimed at the determination of a new precision, which will soon be carried out. In addition, the intermediate precision, limit of detection (LD) and limit of quantitation (LQ) of the method were determined. The method was verified by examining 139 samples of commercial LPG fuel. The tested samples were eval- uated for compliance with the specifications from 2012 and 2018. It was found that by testing samples according to EN 17178:2019, the criterion for assessing compliance with the requirements of the specification on LPG is tightened compared to testing according to the ASTM D 6667-14 standard.

Źródło:

Nafta-Gaz; 2020, 76, 8; 540-549
0867-8871

Pojawia się w:

Nafta-Gaz

Dostawca treści:

Biblioteka Nauki

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Skocz do pozycji: 37.

Tytuł:

Fluorescence optical analysis method for assessing homogeneity of granular mixtures

Autorzy:

Matuszek, Dominika B.
Królczyk, Jolanta B.

Powiązania:

https://bibliotekanauki.pl/articles/1848991.pdf

Data publikacji:

2021

Wydawca:

Polska Akademia Nauk. Czytelnia Czasopism PAN

Tematy:

fluorescence
ultraviolet radiation
homogeneity
multicomponent granular mixtures
tracer

Opis:

This paper presents a method of optical fluorescence analysis for the evaluation of homogeneity of multicomponent grain mixtures. This method is based on the evaluation of the content of fluorescent marker. Maize with two degrees of fineness d1 = 1.25 mm and d2 = 2.00 mm was used as a tracer. Maize was covered with Rhodamine B, which emits red light under the influence of ultraviolet radiation. The tracer was introduced into the mixture before the mixing process began. Nine multicomponent grain mixtures were used. The proportion of fluorescent maize was evaluated on the basis of computer image analysis. Additionally, the fraction of the tracer was evaluated using a control method (validation of the accuracy of the proposed method). The results indicate that the degree of the tracer’s fineness influences the results obtained. The use of fluorescent maize with particle size d2 = 2.00 mm allowed to obtain results which differed less from the control method. The average size of the difference in results ranged from 0.20-0.38 for the 2.00 mm tracer and 0.38-1.34 for the 1.25 mm tracer.

Źródło:

Metrology and Measurement Systems; 2021, 28, 1; 41-54
0860-8229

Pojawia się w:

Metrology and Measurement Systems

Dostawca treści:

Biblioteka Nauki

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Skocz do pozycji: 38.

Tytuł:

Impact of a Longwave UV-B Radiation on Soybean Plants Grown at Increased Nickel Concentration in Soil

Autorzy:

Skórska, Elżbieta
Matuszak-Slamani, Renata
Romanowski, Hubert
Szwarc, Wiktor

Powiązania:

https://bibliotekanauki.pl/articles/123597.pdf

Data publikacji:

2019

Wydawca:

Polskie Towarzystwo Inżynierii Ekologicznej

Tematy:

chlorophyll fluorescence
flavonoids
Glycine max
photosynthesis
ultraviolet B

Opis:

Plants of soybean (Glycine max L.) cv. Augusta grew in the soil with addition of Ni in the concentration of 80 (C-80) or 120 mg∙kg^-1 dry mass (C-120) or without Ni (C-0), and they were subjected to UV-B (300 nm – 315 nm) dose of 1.8 kJ m^-2 d^-1 for 28 days. The addition of nickel into soil in the concentration of 120 mg kg^-1 (C-120) caused a decrease of the plant height by 35% compared to the control (C-0), and in the presence of UV-B (U-120) – by 43%. The shoot fresh and dry mass in the C-80 and C-120 plants were lower by 33% and 52% than in the C-0. In the presence of Ni, the intensity of net photosynthesis decreased by 55%. UV-B caused an increase of flavonoid content by 25% compared to the control (C-0), and Ni induced a reduction in the content of these compounds from 20% to 40%.

Źródło:

Journal of Ecological Engineering; 2019, 20, 5; 135-141
2299-8993

Pojawia się w:

Journal of Ecological Engineering

Dostawca treści:

Biblioteka Nauki

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Skocz do pozycji: 39.

Tytuł:

Synchronous fluorescence spectra of water contaminated by dispersed crude oil

Autorzy:

Baszanowska, Emilia
Otremba, Zbigniew

Powiązania:

https://bibliotekanauki.pl/articles/174431.pdf

Data publikacji:

2020

Wydawca:

Politechnika Wrocławska. Oficyna Wydawnicza Politechniki Wrocławskiej

Tematy:

petroleum
dispersed oil
synchronous fluorescence spectroscopy
marine environment

Opis:

The development of petroleum extraction and transport technology does not ensure complete isolation of these substances from the natural environment. This problem is exacerbated by the location of mining equipment on the sea shelf and the fact that numerous submarine pipelines, tankers and handling terminals can also emit oil pollution. Therefore, the possibility of detecting oil dispersed in the water is particularly important. This paper reports the efforts to identify methods of characterization of the water containing the crude oil emulsion in a very low concentration (a few to several tens of ppm). Due to this, the effect of emulsion concentration on the possibility of its objective characterization using synchronous fluorescence spectra was studied. The similarity of spectra at various oil concentrations was analysed. It has been shown that the stabilization of the shape of synchronous fluorescence spectra occurs at relatively low oil concentrations.

Źródło:

Optica Applicata; 2020, 50, 1; 161-169
0078-5466
1899-7015

Pojawia się w:

Optica Applicata

Dostawca treści:

Biblioteka Nauki

/5

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Skocz do pozycji: 40.

Tytuł:

Rejestracja śladów daktyloskopijnych systemem TrasoScan
Registration of fingerprints with TrasoScan System

Autorzy:

Rogoża, Ewa
Drzewiecka, Katarzyna

Powiązania:

https://bibliotekanauki.pl/articles/23050945.pdf

Data publikacji:

2021

Wydawca:

Centralne Laboratorium Kryminalistyczne Policji

Tematy:

TrasoScan
ślady daktyloskopijne
fluorescencja śladów
fingerprints
trace fluorescence

Opis:

Ciągły rozwój metod wizualizacji śladów wymusił tworzenie nowych, specjalistycznych urządzeń do ich rejestracji. W ostatnich latach na rynku kryminalistycznym pojawił się system TrasoScan do rejestracji i obróbki śladów kryminalistycznych: traseologicznych, daktyloskopijnych lub dokumentów. Obecnie najczęściej wykorzystywany jest w badaniach traseologicznych. Niemniej jednak z dużym powodzeniem może być zastosowany do rejestracji śladów linii papilarnych na foliach daktyloskopijnych, odlewach silikonowych, zabezpieczonych wraz z podłożem, bądź śladów ujawnionych metodami chemicznymi. W niniejszym artykule przedstawiono szerokie spektrum możliwości TrasoScanu w zakresie rejestracji różnego rodzaju śladów daktyloskopijnych.
The continuous development of methods of trace visualization entailed the development of new, specialized trace registration devices. In recent years, the TrasoScan System for registration and processing of forensic traces: traseological traces, fingerprints or documents has appeared on the forensic market. Currently, this device is most often applied to traseological examinations. Notwithstanding the above, it can be successfully used to register fingerprints on dactyloscopic films, silicone casts, those secured together with a surface, or traces revealed by chemical methods. This article presents the broad spectrum of TrasoScan’s capabilities for registering fingerprints of various types.

Źródło:

Problemy Kryminalistyki; 2021, 313; 13-19(pol), 42-48(eng)
0552-2153

Pojawia się w:

Problemy Kryminalistyki

Dostawca treści:

Biblioteka Nauki

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Skocz do pozycji: 41.

Tytuł:

Does fish stocking rate affect the photosynthesis of Lactuca sativa grown in an aquaponic system?

Autorzy:

Jaszczuk, Zuzanna Malwina
Brysiewicz, Adam
Kozioł, Agnieszka
Auriga, Alicja
Brestic, Marian
Kalaji, Hazem M.

Powiązania:

https://bibliotekanauki.pl/articles/28411613.pdf

Data publikacji:

2023

Wydawca:

Instytut Technologiczno-Przyrodniczy

Tematy:

aquaponics
chlorophyll fluorescence
common carp
lettuce
plant production

Opis:

The depletion of natural resources such as freshwater and cropland makes it necessary to find a new solution for sustainable food production. Aquaponic systems seem to be a great alternative to traditional agriculture, however, there are still many unknowns that need to be explored. It is already known how fish stocking affects water quality in aquaponic systems, but not how it affects the plants’ growth, and especially on chlorophyll fluorescence. In this study, we examined how the density of 0, 2, 4, 8, and 16 stocking fish in five aquaria affects lettuce growth. The first tank was only a hydroponic system with plants but without fish (control). In the remaining four aquaria - 2, 4, 8 and 12 specimens of common carp fry with an average weight of 20 grams (average 8.5-33.2 g) were placed in the aquaponic growing system. Physicochemical analysis of water was conducted to determine the levels of pH, electrical conductivity (EC), N-NO3, N-NO2, N-NH4, P-PO4, O2 and physiological parameters of plants (nitrogen balance index - NBI, chlorophyll content index - CCI, quantum yield - QY, flavonoid content - Flv) were analysed. The results showed that fish stocking density has different effects on plant physiological parameters, but in most cases, was insignificant. It seems that the greater number of fishes and higher density indirectly causes growth inhibition (lower photosynthetic efficiency) due to the increase of N-NO3 and a decrease of O2 in the water.

Źródło:

Journal of Water and Land Development; 2023, 58; 243--252
1429-7426
2083-4535

Pojawia się w:

Journal of Water and Land Development

Dostawca treści:

Biblioteka Nauki

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Skocz do pozycji: 42.

Tytuł:

Spectral properties of phthalocyanines incorporated into resting and stimulated human peripheral blood cells.

Autorzy:

Waszkowiak, Aneta
Frąckowiak, Danuta
Wiktorowicz, Krzysztof
Miyake, Jun

Powiązania:

https://bibliotekanauki.pl/articles/1043724.pdf

Data publikacji:

2002

Wydawca:

Polskie Towarzystwo Biochemiczne

Tematy:

phthalocyanines
fluorescence
photodynamic therapy
human peripheral blood cells

Opis:

Human peripheral blood cells stimulated by phytohemagglutinin (which serve as a model of cancerous cells) and resting cells were incubated in dimethyl sulfoxide solutions of various phthalocyanines. In order to diminish the influence of atmospheric oxygen the cells were embedded in a polymer (polyvinyl alcohol) film. Fluorescence spectra of the samples were measured over two regions of excitation wavelengths: at 405 nm (predominant absorption of the cell material) and in the regions of strong absorption of phthalocyanines (at about 605 nm and 337 nm). The intrinsic emission of cell material became changed as a result both of cells' stimulation and of incubation of cells in dye solution. In most cases the stimulated cells when stained by dye exhibited higher long wavelength fluorescence intensity than resting cells. This suggests higher efficiency of dye incorporation into cancerous cells than into healthy cells. The absorption spectra of samples were also measured. The spectra of various phthalocyanines in incubation solvent, in polymer and in the cells embedded in polymer, were compared. The comparison of properties of the cells stimulated for different time periods enabled to establish the conditions of stimulation creating a population of cells incorporating a large number of sensitizing molecules.

Źródło:

Acta Biochimica Polonica; 2002, 49, 3; 633-641
0001-527X

Pojawia się w:

Acta Biochimica Polonica

Dostawca treści:

Biblioteka Nauki

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Skocz do pozycji: 43.

Tytuł:

Insight into the kinetics and the mode of the interaction between smooth muscle calponin and F-actin.

Autorzy:

Kołakowski, Janusz
Dąbrowska, Renata

Powiązania:

https://bibliotekanauki.pl/articles/1043785.pdf

Data publikacji:

2002

Wydawca:

Polskie Towarzystwo Biochemiczne

Tematy:

light scattering
fluorescence
actin-binding
calponin
stopped-flow

Opis:

Kinetics of the smooth muscle calponin-F-actin interaction was studied by stopped- flow measurements of light scattering and fluorescence intensity of pyrene-labelled F-actin. The intensity and character of the changes in light scattering, and thus the mode of calponin binding to actin filaments leading to changes in their shape and bundling, depend on the molar ratio of the two proteins. Parallel measurements of pyrene-fluorescence quenching upon calponin binding revealed that intrinsic conformational changes in actin filaments are delayed relative to the binding process and are not markedly influenced by the mode of calponin binding. Bundling of actin filaments by calponin was not correlated with fluorescence changes and thus with alterations in the structure of actin filaments.

Źródło:

Acta Biochimica Polonica; 2002, 49, 2; 471-479
0001-527X

Pojawia się w:

Acta Biochimica Polonica

Dostawca treści:

Biblioteka Nauki

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Skocz do pozycji: 44.

Tytuł:

A Fluorescence, 1H NMR spectroscopy and molecular dynamics study of the influence of rotamer population on fluorescence decay of tyrosine, phenylalanine and their derivatives

Autorzy:

Ganzynkowicz, R.
Liwo, A.
Wiczk, W.

Powiązania:

https://bibliotekanauki.pl/articles/1965825.pdf

Data publikacji:

2001

Wydawca:

Politechnika Gdańska

Tematy:

tyrosine
phenylalanine
rotamers
fluorescence
molecular dynamics
NMR spectroscopy

Opis:

Molecular dynamics simulations were carried out on tyrosine and phenylalanine and their derivatives with various terminal groups to determine the populations of side-chain rotamers. The obtained populations were compared with those calculated from fluorescence-decay lifetime distributions and NMR studies. It was found that theoretically calculated populations do not match the experimental ones, which suggests that the static rotamer model is inadequate to explain the dynamics of tyrosine and phenylalanine side chain in fluorescence and NMR experiments.

Źródło:

TASK Quarterly. Scientific Bulletin of Academic Computer Centre in Gdansk; 2001, 5, 3; 311-316
1428-6394

Pojawia się w:

TASK Quarterly. Scientific Bulletin of Academic Computer Centre in Gdansk

Dostawca treści:

Biblioteka Nauki

/5

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Skocz do pozycji: 45.

Tytuł:

Palynofacies analysis, sedimentology and hydrocarbon potential of the Menilite Beds (Oligocene) in the Slovakian and Romanian Outer Carpathians

Autorzy:

Filipek, Anna

Powiązania:

https://bibliotekanauki.pl/articles/2060248.pdf

Data publikacji:

2020

Wydawca:

Państwowy Instytut Geologiczny – Państwowy Instytut Badawczy

Tematy:

Menilite Beds
palynofacies
UV fluorescence
thermal maturity
Carpathians

Opis:

The sedimentary organic matter (SOM) assemblages and sedimentology of the Menilite Beds from the Dukla, Grybów and Vrancea units in the Slovakian and Romanian Outer Carpathians are described. Qualitative and quantitative analyses of the SOM help ascertain depositional conditions, while the thermal maturity of the organic matter studied is estimated utilizing the Spore Colour Index and UV light excitation techniques. The sedimentary organic particles were grouped into ten SOM categories: marine palynomorphs (dinoflagellate cysts), sporomorphs (saccate and non-saccate, pollen and spores), freshwater algae (Botryococcus sp., and other freshwater microplankton), phytoclasts (cuticles, translucent wood, opaque wood), resin and amorphous organic matter (AOM). All samples are dominated by AOM. The presence of Botryococcus sp., Pediastrum sp., Pterospermella sp. and Campenia sp., in some samples points to deposition under hyposaline conditions. It is interpreted that the freshwater influx induced water column stratification in the basin, leading to the development of dysoxic to anoxic bottom-water conditions that enhanced the preservation of AOM. Kerogen analysis in UV light and evaluation using the Spore Colour Index demonstrated different thermal maturation patterns from the Slovakian (post-mature) and Romanian (immature) sections. Integrated palynofacies analysis (notably, the presence of freshwater algae) and sedimentological observations (e.g., hummocky cross-stratification) lead to the conclusion that the deposition of the Menilite Beds in the Vrancea Unit (Romania) was relatively proximal to the shoreline, above storm wave base, whereas the Slovakian units (Dukla and Grybów) were deposited in a more distal setting.

Źródło:

Geological Quarterly; 2020, 64, 3; 589--610
1641-7291

Pojawia się w:

Geological Quarterly

Dostawca treści:

Biblioteka Nauki

/5

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Skocz do pozycji: 46.

Tytuł:

Fluorescence of natural seawater exposed to oil pollution

Autorzy:

Otremba, Z.
Baszanowska, E.

Powiązania:

https://bibliotekanauki.pl/articles/245422.pdf

Data publikacji:

2015

Wydawca:

Instytut Techniczny Wojsk Lotniczych

Tematy:

marine fleet exploitation
seawater
oil pollution
fluorescence spectroscopy

Opis:

The natural seawater contains both dissolved and suspended organic substances originating from natural sources and human activities – like the marine transport fleet among other. To specify the type and quantity of vitally valid as well as dangerous for properly functioning marine ecosystems substances complicated and sophisticated chemical instrumentation and methodologies must be used. Only a small number of seawater components it is possible easily to determine their concentrations – for example, the salt content is determined directly in the bulk of water through simultaneous measurement of electrolytic conductivity and temperature of water. It is worth to search similarly quick method for oil substances directly in the seawater. Taking into account seawater organic pollutants originating from natural sources inter alia crude oils, the presence of refinery petroleum substances due to their fluorescence in ultra-violet light can be determined based on fluorescence spectroscopy. The aim of this paper is to search fluorescence features of oil substances dissolved in natural seawater based on excitation-emission spectroscopy. In the paper fresh and oily contaminated seawater taken from the coastal area of the Baltic Sea were used. As an oil pollution ‘Petro-baltic’ oil were applied. The natural seawater, at first was laboratory exposed to low extremely amount of oil and then it was examined by fluorescence under UV light. The seawater from vicinity of Gdynia (Poland) were tested as fresh and after artificially contaminated by different amount of oil (from 0.5 to 500 ppm). Spectrofluorometer Hitachi F-7000 FL was applied to measure excitation-emission spectra (EEMs). Low amounts of oil (up to several ppm) cause increasing of main peak in excitation-emission spectra (EEMs). Addition of larger amounts of oil results in appearance new peaks, which originate from fluorescence of soluble fractions of oil artificially added to examined water. These specific features of oil describe the spectroscopic signatures of oil, which is the basis to development operational method of the source of oil pollution identification.

Źródło:

Journal of KONES; 2015, 22, 3; 181-186
1231-4005
2354-0133

Pojawia się w:

Journal of KONES

Dostawca treści:

Biblioteka Nauki

/5

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Skocz do pozycji: 47.

Tytuł:

Spectrofluorymetry in application to oil-in-water emulsion characterization

Autorzy:

Otremba, Z.
Baszanowska, E.
Toczek, H.

Powiązania:

https://bibliotekanauki.pl/articles/246063.pdf

Data publikacji:

2011

Wydawca:

Instytut Techniczny Wojsk Lotniczych

Tematy:

oil
fuels
lubricants
aquatic environment protection
fluorescence spectra

Opis:

The question of identifying the type of oil in exploitation of engine room is associated with both the quality of ship consumables (fuels and lubricants) and the composition and origin of oil in settling tanks or in dumping water. Related issue is the possibility of determining the origin of oil, which is detected in the marine environment. The key question is how oils vary in their ability to fluorescence, understood as the shapes of fluorescence spectra obtained for different wavelengths of light exciting fluorescence. In this paper we present spectra of fluorescence for six chosen oils. Those oils were previously dispersed (emulsified) in the seawater, then extracted into hexane. Fluorescence spectra were obtained using fluorescence spectrometer Perkin Elmer LS55, for excitation wavelengths in the range from 240 nm to 500 nm, and emission wavelengths from 300 nm to 790 nm. In this paper there is shown that in general both total intensity of fluorescence and shapes of spectra of fluorescence depend on the excitation wavelength and are differ for various kinds of oil. In order to visually show the differences in fluorescence abilities of various oils, the results of measurement were placed on a chart of fluorescence intensities in the function of both variables: the excitation wavelength and the emission wavelength (so called the total fluorescence spectra). Analyses of results of the described studies confirm that the different oils fluoresce differently and identification of type of oil is possible by fluorescence spectrophotometry.

Źródło:

Journal of KONES; 2011, 18, 3; 317-321
1231-4005
2354-0133

Pojawia się w:

Journal of KONES

Dostawca treści:

Biblioteka Nauki

/5

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Skocz do pozycji: 48.

Tytuł:

Comparison of fluorometric signatures of oil residues in both marine environment and in organic diluent

Autorzy:

Otremba, Z.
Baszanowska, E.

Powiązania:

https://bibliotekanauki.pl/articles/243211.pdf

Data publikacji:

2015

Wydawca:

Instytut Techniczny Wojsk Lotniczych

Tematy:

oil pollution
seawater
excitation-emission spectra
fluorescence spectroscopy

Opis:

The significant part of petroleum substances is transferred to the marine environment after technical activities (inevitable exploitive spills, illegal discharges). However a certain part of petroleum in seawater coming from the natural sources. Therefore, to develop the safety of natural marine environment it is necessary to track not only the anthropogenic pollutants but also the natural sources of petroleum. To distinguish both source of oil the fluorescence properties of oil can be used as the tools to oil identification. The aim of the study is to describe the optical properties of crude oil after it contact with aquatic environment based on fluorescence spectroscopy. For the study, crude oil ‘Petrobaltic’ type potentially found in marine environment due to coming from natural source was applied. To dissolve oil, two different solvent – hexane and Baltic seawater form the Gdynia coast were used. To measure the excitation-emission spectra (EEMs) of oil spectrofluorometer Hitachi F-7000 FL was applied. In the paper, we discuss the changes of oil EEMs in two cases for oil dissolved in n-hexane and for oil dissolved in seawater for various oil concentration. Obtained EEMs for oil dissolved in seawater indicate that EEMs spectrum contain the main peak – describing by the wavelength – independent fluorescence maximum, which was detected for oil dissolved in n-hexane (typical for this kind of oil). However, the intensity of oil fluorescence decreases after oil contact with seawater. This approach could allow expanding the description of complex mixtures of oils as a possible tool proposed for identification the type and origin of oil found in marine environment.

Źródło:

Journal of KONES; 2015, 22, 3; 175-179
1231-4005
2354-0133

Pojawia się w:

Journal of KONES

Dostawca treści:

Biblioteka Nauki

/5

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Skocz do pozycji: 49.

Tytuł:

Application of X-ray fluorescence techniques for the determination of hazardous and essential trace elements in environmental and biological materials

Autorzy:

Bamford, S.
Wegrzynek, D.
Chinea-Cano, E.
Markowicz, A.

Powiązania:

https://bibliotekanauki.pl/articles/147255.pdf

Data publikacji:

2004

Wydawca:

Instytut Chemii i Techniki Jądrowej

Tematy:

biological material
environmental materials
X-ray fluorescence analysis

Opis:

The utilization of X-ray fluorescence technique for the determination of trace element concentrations in environmental and biological samples is presented. The analytical methods used include energy dispersive X-ray fluorescence with polarizing secondary targets, total reflection X-ray fluorescence, direct in-situ X-ray fluorescence, and micro-beam X-ray fluorescence spectrometry. These methods were applied to analysis of different samples including soil, water, plant material and airborne particulate matter collected on polycarbonate filters. The performance and achieved detection limits of elements for different techniques, established by measuring appropriate reference standards, are presented. Also described is the utilization of micro-beam X-ray fluorescence technique for studying element distribution in heterogeneous samples and investigating the 2D- and 3D-morphology of minute samples by means of computerized X-ray absorption and X-ray fluorescence tomography. The different X-ray techniques have their unique advantages. The micro-beam X-ray fluorescence set-up has an advantage of producing very well collimated primary X-ray beam (about 15 mm in diameter), in front of which the analyzed sample can be precisely positioned, providing local information about the sample composition. The energy dispersive X-ray fluorescence with secondary targets offers rapid analysis of broad range of elements (Na–U) combined with a simple sample preparation method. Total reflection X-ray fluorescence technique, characterized by the lowest detection limits of elements, has its leading edge in analysis of liquid samples, and dealing with particle size effects in air particulates collected on filter papers. In-situ X-ray fluorescence spectrometers are truly portable and enable on the spot, in field analysis. It is shown that the combination of several XRF methods

Źródło:

Nukleonika; 2004, 49, 3; 87-95
0029-5922
1508-5791

Pojawia się w:

Nukleonika

Dostawca treści:

Biblioteka Nauki

/5

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Skocz do pozycji: 50.

Tytuł:

Fluorymetryczny detektor kokainy w ludzkim pocie - przenośny narkometr nowej generacji
Flurometric detector of cocaine in a sweat sample - a new generation portable narcometer

Autorzy:

Walczak, R.
Dziuban, J.
Kruger, J.

Powiązania:

https://bibliotekanauki.pl/articles/154811.pdf

Data publikacji:

2010

Wydawca:

Stowarzyszenie Inżynierów i Techników Mechaników Polskich

Tematy:

test paskowy
kokaina
fluorescencja
lateral flow test
cocaine
fluorescence

Opis:

Wykrycie kokainy zażytej przez zawodowego kierowcę jest tak samo ważne jak przeprowadzenie testu na spożycie alkoholu. Skutki zażycia jednej i drugiej używki mogą być tragiczne. Dlatego też, istotne jest wykrycie kokainy lub jej metabolitów, za pomocą prostych w obsłudze i tanich testów, nie tylko w chwili upojenia tym narkotykiem. Pozwoli to na stwierdzenie, że kierowca w czasie pracy (jazdy samochodem) był pod wpływem kokainy. Brak jest w chwili obecnej tego typu testów. W artykule przedstawiono wyniki prac prowadzonych w ramach 7. PR UE LABONFOIL nad tanim testem na obecność kokainy lub jej metabolitów w pocie kierowcy. Test ten bazuje na jednorazowym pasku immunochromatograficznym współpracującym z ręcznym czytnikiem optycznym. Zastosowanie nowatorskiego układu detekcji optycznej umożliwia wykrycie metabolitu kokainy na poziomie do tej pory charakterystycznym dla stacjonarnych urządzeń laboratoryjnych, otwierając tym samym drogę w kierunku nowej generacji przenośnych narkometrów.
Detection of cocaine taken by a professional driver is from safety reasons as dangerous as consumption of alcohol. Therefore, it is crucial to detect cocaine or its metabolite by the use of simple and cheap test not only while the driver is abused. The paper presents a first approach toward portable instrumentation for cheap and rapid quantitative detection of cocaine in a sweat sample developed under EU 7. FP LABONFOIL project. The test consists of a skin patch and a hand-held optical reader (Fig. 1). The reader is connected to a personal computer which collects data and calculates cocaine concentration in the sweat sample (Fig. 2). Details of the immunochromatographic strip and hand-held reader are shown in Figs. 3 and 4. The view of the hand-held reader connected to an ultra-mobile personal computer is shown in Fig. 5. The reader sends images of the flourishing control and test lines to the computer which analyes and calculates fluorescence intensity of the lines (Figs. 6, 7). During experiments immunochromatographic tests with cocaine metabolite concentration corresponding to cocaine concentration from 0 ng/ml to 20 ng/ml were used. The detection areas for different concentrations are presented in Table 1. The fluorescence intensity as a function of the cocaine metabolite concentration is shown in Fig. 8. The detection limit of cocaine was about 2 ng/ml,m which is at least 10-times better than the limit recommended by EC (30 ng/ml).

Źródło:

Pomiary Automatyka Kontrola; 2010, R. 56, nr 7, 7; 812-815
0032-4140

Pojawia się w:

Pomiary Automatyka Kontrola

Dostawca treści:

Biblioteka Nauki

/5

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Skocz do pozycji: 51.

Tytuł:

Effect of excitation migration on the decay of excited states via electron transfer to acceptors

Autorzy:

Gutman, M.
Hilczer, M.
Tachiya, M.

Powiązania:

https://bibliotekanauki.pl/articles/147856.pdf

Data publikacji:

2005

Wydawca:

Instytut Chemii i Techniki Jądrowej

Tematy:

electron transfer
energy migration
simulation
fluorescence decay
polymer matrix

Opis:

Simulation of fluorescence quenching due to electron transfer from a photoexcited donor to an acceptor, in a rigid matrix has been performed. The model assumes competition between processes of the electron transfer and the resonant excitation energy transfer among donors. Electron and energy transfers are described in terms of the Marcus and the Förster theories, respectively. Dependence of the fluorescence quenching on the donor concentration and on the parameters of the Marcus and Förster equations has been investigated at a fixed acceptor concentration. Fluorescence decay curves calculated for various concentrations of donors have been compared with experimental decays.

Źródło:

Nukleonika; 2005, 50,suppl.2; 39-43
0029-5922
1508-5791

Pojawia się w:

Nukleonika

Dostawca treści:

Biblioteka Nauki

/5

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Skocz do pozycji: 52.

Tytuł:

A new cyclotriveratrylene derivative as turn-off fluorescent probe with quinoline appended arm for the selective and sensitive determination of Cu2+ ions

Autorzy:

Fernandes, Patrick F.
Mishra, Divya R.

Powiązania:

https://bibliotekanauki.pl/articles/1030477.pdf

Data publikacji:

2020

Wydawca:

Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN

Tematy:

8-quniolinesulfonyl chloride
Fluorescence studies
Sensors
copper ions
cyclotriveratrylene

Opis:

A novel supramolecular fluorescent molecular probe constructed on cyclotriveratrylene based derivative allied to 8-quinolinesulfonyl chloride (8QSC) was synthesized and used for the detection of copper ions among the various cations. Using 1H-NMR, 13C-NMR, and ESI-MS techniques the compound was successfully characterised. Furthermore the complexation behaviour of 8QSC-CTV with different cations were studied. A shift in the absorption spectra showed the detection of copper ion with the ligand 8QSC-CTV which concludes the strong binding interaction between the ligand and the cation. Furthermore the metal ion showed quenching in the emission spectra. The quantum yield of the metal complex was also studied. The stern volmer analysis concluded that the mechanism of fluorescence quenching is either purely dynamic or purely static.

Źródło:

World Scientific News; 2020, 144; 103-113
2392-2192

Pojawia się w:

World Scientific News

Dostawca treści:

Biblioteka Nauki

/5

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Skocz do pozycji: 53.

Tytuł:

Wyniki analiz spektrometrycznych dwóch dzbanów fenickich z kolekcji gołuchowskiej

Autorzy:

Krueger, Michał
Głuszek, Inga

Powiązania:

https://bibliotekanauki.pl/articles/1023876.pdf

Data publikacji:

2018-07-31

Wydawca:

Uniwersytet im. Adama Mickiewicza w Poznaniu

Tematy:

X-ray fluorescence spectrometry analyses
Phoenician jugs
Gołuchów collection

Opis:

This paper presents results of the spectrometric analyses of two Phoenician jugs from the Gołuchów collection. A non-invasive portable X-ray fluorescence spectrometer (pXRF) has been used to determine the chemical composition of the jugs. The aim of this work was to add new results to a database of spectrometric data of Phoenician pottery from central and western Mediterranean. Good agreement obtained between the chemical results of the two jugs suggest  that they could have been made from the same clay.

Źródło:

Folia Praehistorica Posnaniensia; 2017, 22; 271-285
0239-8524
2450-5846

Pojawia się w:

Folia Praehistorica Posnaniensia

Dostawca treści:

Biblioteka Nauki

/5

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Skocz do pozycji: 54.

Tytuł:

Thermodynamics of specific protein-RNA interactions.

Autorzy:

Stolarski, Ryszard

Powiązania:

https://bibliotekanauki.pl/articles/1043600.pdf

Data publikacji:

2003

Wydawca:

Polskie Towarzystwo Biochemiczne

Tematy:

calorimetry
thermodynamics
fluorescence
proteins
specific binding
RNA 5' cap

Opis:

Description of the recognition specificity between proteins and nucleic acids at the level of molecular interactions is one of the most challenging tasks in biophysics. It is key to understanding the course and control of gene expression and to the application of the thus acquired knowledge in chemotherapy. This review presents experimental results of thermodynamic studies and a discussion of the role of thermodynamics in formation and stability of functional protein-RNA complexes, with a special attention to the interactions involving mRNA 5' cap and cap-binding proteins in the initiation of protein biosynthesis in the eukaryotic cell. A theoretical framework for analysis of the thermodynamic parameters of protein-nucleic acid association is also briefly surveyed. Overshadowed by more spectacular achievements in structural studies, the thermodynamic investigations are of equal importance for full comprehension of biopolymers' activity in a quantitative way. In this regard, thermodynamics gives a direct insight into the energetic and entropic characteristics of complex macromolecular systems in their natural environment, aqueous solution, and thus complements the structural view derived from X-ray crystallography and multidimensional NMR. Further development of the thermodynamic approach toward interpretation of recognition and binding specificity in terms of molecular biophysics requires more profound contribution from statistical mechanics.

Źródło:

Acta Biochimica Polonica; 2003, 50, 2; 297-318
0001-527X

Pojawia się w:

Acta Biochimica Polonica

Dostawca treści:

Biblioteka Nauki

/5

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Skocz do pozycji: 55.

Tytuł:

Ca2+differently affects hydrophobic properties of guanylyl cyclase-activating proteins (GCAPs) and recoverin.

Autorzy:

Gorczyca, Wojciech
Kobiałka, Marcin
Kuropatwa, Marianna
Kurowska, Ewa

Powiązania:

https://bibliotekanauki.pl/articles/1043612.pdf

Data publikacji:

2003

Wydawca:

Polskie Towarzystwo Biochemiczne

Tematy:

fluorescence
calcium-binding proteins
recoverin
guanylyl cyclase-activating proteins

Opis:

Guanylyl cyclase-activating proteins (GCAPs) and recoverin are retina-specific Ca2+-binding proteins involved in phototransduction. We provide here evidence that in spite of structural similarities GCAPs and recoverin differently change their overall hydrophobic properties in response to Ca2+. Using native bovine GCAP1, GCAP2 and recoverin we show that: i) the Ca2+-dependent binding of recoverin to Phenyl-Sepharose is distinct from such interactions of GCAPs; ii) fluorescence intensity of 1-anilinonaphthalene-8-sulfonate (ANS) is markedly higher at high [Ca2+]gfree (10 μM) than at low [Ca2+]free (10 nM) in the presence of recoverin, while an opposing effect is observed in the presence of GCAPs; iii) fluorescence resonance energy transfer from tryptophane residues to ANS is more efficient at high [Ca2+]free in recoverin and at low [Ca2+]free in GCAP2. Such different changes of hydrophobicity evoked by Ca2+ appear to be the precondition for possible mechanisms by which GCAPs and recoverin control the activities of their target enzymes.

Źródło:

Acta Biochimica Polonica; 2003, 50, 2; 367-376
0001-527X

Pojawia się w:

Acta Biochimica Polonica

Dostawca treści:

Biblioteka Nauki

/5

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Skocz do pozycji: 56.

Tytuł:

Spectrofluorimetry of chosen distillates of oil after pyrolysis of scrap tires

Autorzy:

Otremba, Z.
Ambrosewicz-Walacik, M.

Powiązania:

https://bibliotekanauki.pl/articles/245271.pdf

Data publikacji:

2018

Wydawca:

Instytut Techniczny Wojsk Lotniczych

Tematy:

fuels
used car tires
pyrolysis
fluorescence
excitation-emission spectra

Opis:

One of way to obtain liquid hydrocarbons can be pyrolysis of scrap tires. The hydrocarbon substances produced by this method may be an addition to traditional fuels, i.e. gasolines or fuels for diesel engines. In laboratory conditions at Department of Mechatronics of University of Warmia and Mazury there were obtained pyrolytic products and prepared three distillates related to temperatures 160, 204 and 350ºC. Analyses of shapes of fluorescence spectra were carried out in Department of Physics of Gdynia Maritime University. Spectra of fluorescence were prepared applying the spectrofluorimeter Hitachi F-7000 FL, which allows applying of excitation wavelength from 200 nm until 600 nm, whereas analysing of emitted, can be performed until 750 nm. In here reported study excitation and emission range for the most intense fluorescence are presented. There were prepared four solutions of pyrolytic products in the n-hexane: 4 ppm, 20 ppm, 100 ppm and 500 ppm. In order to show dependence of intensity fluorescence and shapes of excitation-emission spectra on type of distillate and concentration both kind of visualization – three-dimensional and as contour maps – are shown in this article. In analysed range of wavelengths, intensity of fluorescence grows with concentration of oil, whereas structure of the shape of spectra simplify when concentration increases. The presented effects associated with the fluorescence of pyrolysis products allow to predict the possibility of developing a method for determining the content of these substances in mixtures with petroleum refining products and other liquid hydrocarbons.

Źródło:

Journal of KONES; 2018, 25, 2; 262-268
1231-4005
2354-0133

Pojawia się w:

Journal of KONES

Dostawca treści:

Biblioteka Nauki

/5

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Skocz do pozycji: 57.

Tytuł:

Analysis of adsorption of heavy metals from water solutions by wood of selected domestic species using X-Ray Fluorescence (XRF)

Autorzy:

Balicka, Adrianna
Szadkowski, Jan

Powiązania:

https://bibliotekanauki.pl/articles/2200144.pdf

Data publikacji:

2020

Wydawca:

Szkoła Główna Gospodarstwa Wiejskiego w Warszawie. Wydawnictwo Szkoły Głównej Gospodarstwa Wiejskiego w Warszawie

Tematy:

X-Ray Fluorescence Spectroscopy
wood shaving
adsorption
heavy metals

Opis:

Analysis of adsorption of heavy metals from water solutions by wood of selected domestic species using X-Ray Fluorescence (XRF).The aim of this study was to analyze the absorption of three specific heavy metals from model water solution by wood species from domestic Polish forests. This paper focuses on XRF method to determine metal content in samples. European aspen (Populus tremula L.) and Black locust (Robinia pseudoacacia L.) have been chosen. Firstly, shavings were prepared and soaked with standard solutions of lead (II) nitrate, cadmium nitrate and mercury (II) chloride for 7 days. Then, the material was dried and reduced to ashes using muffle furnace. Finally, content of absorbed metal was marked (XRF) and analyzed with a view to initial contents of metal ions in standard solutions. It was established, that the higher the concentration of mercury in standard solution is, the higher impulse counts is obtained for European aspen. This reversal of the dependence is noticeable for second analyzed species.
Badanie adsorpcji metali ciężkich z modelowych roztworów wodnych przez drewno wybranych gatunków krajowych za pomocą Spektrometrii Fluorescencji Rentgenowskiej (XRF). Celem wykonanych badań było sprawdzenie poziomu adsorpcji wytypowanych metali ciężkich tj. ołowiu, kadmu oraz rtęci, przez dwa gatunki rodzime topolę (Populus tremula L.) oraz robinię akacjową (Robinia pseudoacacia L.). W fazie przygotowawczej materiał został znacznie rozdrobniony i rozdzielony na kilka frakcji. Działanie to miało na celu zwiększenie powierzchni adsorpcji badanego drewna. Azotan kadmu (II), azotan ołowiu oraz chlorek rtęci (II) zostały rozpuszczone w destylowanej wodzie w takich proporcjach, aby stworzyć po cztery roztwory – każdy o innym stężeniu. Wybrana frakcja wiórów została poddana obróbce termicznej w wodzie destylowanej w celu usunięcia zawartego powietrza. Następnie frakcję podzielono na próbki 1 gramowe. Drewno nasiąkało w modelowych roztworach przez ok. 168 godzin. Finalnie zostało ono odsączone, wysuszone i spopielone w piecu muflowym. Tak przygotowane próbki zostały poddane badaniu z wykorzystaniem spektrometrii fluorescencji rentgenowskiej (XRF). Urządzenie analizowało zawartość metali w pyle za pomocą dwóch różnych programów i trzech czasów naświetlania. Otrzymane wartości analizowano pod kątem ilościowym a nie jakościowym.

Źródło:

Annals of Warsaw University of Life Sciences - SGGW. Forestry and Wood Technology; 2020, 111; 73--82
1898-5912

Pojawia się w:

Annals of Warsaw University of Life Sciences - SGGW. Forestry and Wood Technology

Dostawca treści:

Biblioteka Nauki

/5

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Skocz do pozycji: 58.

Tytuł:

Neurotransmitters detection using a fluorescence-based sensor with graphene quantum dots

Autorzy:

Baluta, S.
Cabaj, J.
Malecha, K.

Powiązania:

https://bibliotekanauki.pl/articles/175014.pdf

Data publikacji:

2017

Wydawca:

Politechnika Wrocławska. Oficyna Wydawnicza Politechniki Wrocławskiej

Tematy:

sensor
dopamine
fluorescence
Förster resonance energy transfer
quantum dots

Opis:

In the paper, the development and performance of an optical sensor for detection of neurotransmitters (dopamine) is presented. The concentration of dopamine is measured basing on fluorescence quenching of graphene quantum dots. In the sensor, the dopamine molecules coat the graphene quantum dots surface – in result, the quenching of fluorescence occurs due to the Förster resonance energy transfer. The changes in fluorescence correspond to specific concentrations of the neurotransmitter in tested samples, so it is possible to accurately determine the concentration of dopamine in the sample.

Źródło:

Optica Applicata; 2017, 47, 2; 225-231
0078-5466
1899-7015

Pojawia się w:

Optica Applicata

Dostawca treści:

Biblioteka Nauki

/5

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Skocz do pozycji: 59.

Tytuł:

Badania widm absorpcji UV i fluorescencji pyłków roślin
Investigation of UV absorption and emission spectra of pollen

Autorzy:

Kwaśny, M.
Włodarski, M.
Kaliszewski, M.
Bombalska, A.
Mularczyk-Oliwa, M.
Kopczyński, K.

Powiązania:

https://bibliotekanauki.pl/articles/209642.pdf

Data publikacji:

2012

Wydawca:

Wojskowa Akademia Techniczna im. Jarosława Dąbrowskiego

Tematy:

pyłki roślinne
spektroskopia UV
fluorescencja
pollen
spectroscopy UV
fluorescence

Opis:

Przeprowadzono pomiary widm absorpcyjnych i emisyjnych szesnastu pyłków roślin i ich analizę metodą składników głównych (PCA). Praca prezentuje wyniki badań metodą fluorescencji wzbudzanej laserowo (LIF) linią 355 nm i map wzbudzeniowo-emisyjnych. Silna absorpcja promieniowania przy długości fali 340 jest charakterystyczna dla pyłków w przeciwieństwie do białek, bakterii, grzybów. Metoda PCA daje silne zróżnicowanie własności spektralnych tych grup i może być wykorzystana do szybkiego wykrywania mikroorganizmów w powietrzu.
Ultra-violet absorption and emission spectra of 16 pollens were recorded and analysed with PCA (Principal Component Analysis) method. Results of Laser Induced Fluorescence (LIF ) at 355 nm and excitation-emission maps are presented. Pollen presents strong absorption around 340 nm, whereas it was not found for proteins, bacteria and fungi. PCA method provides strong differentiation of spectral properties of biological material and can be used for quick classification of microorganisms in the air.

Źródło:

Biuletyn Wojskowej Akademii Technicznej; 2012, 61, 3; 295-302
1234-5865

Pojawia się w:

Biuletyn Wojskowej Akademii Technicznej

Dostawca treści:

Biblioteka Nauki

/5

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Skocz do pozycji: 60.

Tytuł:

Preliminary studies of phthalocyanine sensitizers incorporated into human leukemia cells from two cell-lines.

Autorzy:

Wiktorowicz, Krzysztof
Cofta, Justyna
Dudkowiak, Alina
Waszkowiak, Aneta
Frąckowiak, Danuta

Powiązania:

https://bibliotekanauki.pl/articles/1041548.pdf

Data publikacji:

2004

Wydawca:

Polskie Towarzystwo Biochemiczne

Tematy:

MOLT 4 cells
fluorescence
photodynamic therapy
phthalocyanine
CCRF cells

Opis:

Three phthalocyanine dyes-sensitizers were incorporated into two types of human T leukemia cells from two cell-lines: CCRF and MOLT 4. The efficiency of the dye incorporation into cells and photochemical properties of stained cells were investigated using fluorescence spectroscopy. The dyes exhibited different properties in each of the two cell-lines. Small differences in cell membrane properties have a strong influence on the efficiency of dye incorporation and on the course of photodynamic reaction. It is suggested that, for a given patient, an optimal dye-sensitizer should be established before photodynamic treatment.

Źródło:

Acta Biochimica Polonica; 2004, 51, 3; 703-710
0001-527X

Pojawia się w:

Acta Biochimica Polonica

Dostawca treści:

Biblioteka Nauki

/5

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Skocz do pozycji: 61.

Tytuł:

Biosensory i sensory fluorescencyjne
Biosensors and fluorescent sensors

Autorzy:

Kłos-Witkowska, A.

Powiązania:

https://bibliotekanauki.pl/articles/151816.pdf

Data publikacji:

2014

Wydawca:

Stowarzyszenie Inżynierów i Techników Mechaników Polskich

Tematy:

biosensor
sensor optyczny
fluorescencja
porfiryna
optical sensor
fluorescence
porphyrin

Opis:

W pracy opisano podstawy działania biosensorów ze szczególnym uwzględnieniem sensorów fluorescencyjnych. Scharakteryzowano metody fluorescencyjne stosowane w badaniach biosensorów oraz sensorów optycznych. Wyjaśniono różnicę pomiędzy biosensorami a sensorami optycznymi wykorzystującymi zjawisko fluorescencji.. Dokonano zestawienia porfiryn oraz wykrywanych przez nie substancji, a także opisano wykorzystanie sensorów fluorescencyjnych w obrazowaniu nowotworów oraz badaniach nad rozwojem leków.
The paper describes the basics of biosensors with particular emphasis on fluorescent sensors (Figs. 1 and 2). There are characterized fluorescent methods used in the studies of biosensors and optical sensors: a change in fluorescence intensity, FRET (Förster resonance energy transfer) FLIM (Fluorescence life time imaging), FCS (Fluorescence correlation spectroscopy). There is explained the difference between biosensors and optical sensors employing the phenomenon of fluorescence. There are presented optical sensors using porphyrins, paying particular attention to porphyrins as substances characterized by the presence of intense absorption bands in the visible light range, high absorption coefficient and intense emission in the visible and infrared range. Due to these properties they are increasingly used in medical diagnostics for imaging the cancer (photodynamic method), and the stage of cancer (diagnosis based on the emission spectra of protoporphyrin IX). In Table 1 there is given the list of porphyrins and detected by them, on the basis of changes in the fluorescence emission spectra , substances: Fe+3, 1O2, O2, Hg+2 , Ca+2, HCl (gas), phospholipids, Pb+2. There is described the use of fluorescent sensors based on porhyrins for cancer imaging and in cancer drug development studies [15, 16, 17].

Źródło:

Pomiary Automatyka Kontrola; 2014, R. 60, nr 1, 1; 3-5
0032-4140

Pojawia się w:

Pomiary Automatyka Kontrola

Dostawca treści:

Biblioteka Nauki

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Skocz do pozycji: 62.

Tytuł:

Lab-on-a-chip fluorescence detection with image sensor and software-based image conditioning

Autorzy:

Walczak, R.

Powiązania:

https://bibliotekanauki.pl/articles/202285.pdf

Data publikacji:

2011

Wydawca:

Polska Akademia Nauk. Czytelnia Czasopism PAN

Tematy:

fluorescence
microfluidics
lab-on-a-chip
point-of-care

Opis:

The fluorometric detection in lab-on-a-chip devices for life-sciences is one of the main detection methods. Well know from “large-scale” analytical chemistry methodologies and instrumentation has been applied in lab-on-a-chip solutions. In most cases, optical instrumentation for fluorescence induction and detection is based on configuration and components borrowed from large laboratory instruments. As a result, optical instrumentation surrounding lab-on-a-chip is bulky, expensive and dedicated for operation only inside laboratory. In this paper a discussion on fluorescence detection in lab-on-a-chip is carried out. A novel image sensor – based detection instrumentation co-working with a “clever” software is described. Instrumentation is dedicated for operation in portable and low-cost devices for different life-science applications. Examples of applications of the novel method and instrumentation co-working with various lab-on-a-chips are presented on the base of author’s works.

Źródło:

Bulletin of the Polish Academy of Sciences. Technical Sciences; 2011, 59, 2; 157-165
0239-7528

Pojawia się w:

Bulletin of the Polish Academy of Sciences. Technical Sciences

Dostawca treści:

Biblioteka Nauki

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Skocz do pozycji: 63.

Tytuł:

A loss of photosynthetic efficiency does not explain stomatal closure in flooded tomato plants
Spadek fotosyntetycznej wydajności nie wyjaśnia zamykania szparek u roślin pomidora w warunkach stresu zalewania

Autorzy:

Janowiak, F.
Else, M.A.
Jackson, M.B.

Powiązania:

https://bibliotekanauki.pl/articles/802005.pdf

Data publikacji:

2002

Wydawca:

Szkoła Główna Gospodarstwa Wiejskiego w Warszawie. Wydawnictwo Szkoły Głównej Gospodarstwa Wiejskiego w Warszawie

Tematy:

photosynthetic efficiency
stoma
chlorophyll fluorescence
flooding stress
tomato plant

Opis:

Stomata begin to close within 24 h of imposing soil flooding. We investigated whether the stomatal response was triggered by reduced photosynthetic efficiency in young, fully expanded leaves of flooded plants. Chlorophyll fluorescence measurements indicated that ФpsII the effective quantum yield of PS II,decreased after stomata began to close in flooded plants. Changes in qP mirrored those of ФpsII, ФpsII was not affected by daytime patterns of stomatal conductance in well-drained plants but was reduced by stomatal closure in flooded plants. Fv/Fm a measure of the overall photosynthetic efficiency of dark- adapted plants, decreased after 57 h of flooding. Therefore, prolonged soil flooding adversely affected the thylakoid membranes. QN, a measure of the amount of captured energy dissipated as heat and therefore, unused by the photosynthetic machinery, began to increase after 32 h of flooding and continued to rise thereafter. The interdependence of the changes in chlorophyll fluorescence parameters and the flooding-induced closure of stomata is discussed.
W warunkach nadmiaru wody w glebie szparki u roślin pomidora zamykają się w czasie 24 h. W prezentowanych eksperymentach badano czy ta reakcja szparek była inicjowana przez spadek fotosyntetycznej wydajności młodych, rozwiniętych liści pomidora. Pomiary fluorescencji chlorofilu wykazały, że ФpsII, efektywna wydajność transportu elektronów PSII, spadała dopiero po rozpoczęciu zamykania szparek u zalewanych roślin. Zmiany qP, fotochemicznego wygaszania fluorescencji, odzwierciedlały przebieg ФpsII Na ФpsII nie wpływał okołodobowy rytm szparek u roślin kontrolnych. Natomiast, u roślin zalewanych, ÖpsII spadało wskutek zamykania szparek. Fv/Fm, miara całkowitej wydajności fotosyntetycznej roślin zaadaptowanych do ciemności, spadał po 57 h zalewania roślin. Wskazuje to na niekorzystny wpływ wydłużonego stresu zalewania na membrany tylakoidowe. Niefotochemiczne wygaszanie fluorescencji (NQ), która jest miarą rozpraszania niewykorzystanej energii w postaci ciepła, wzrastała po 32 h od rozpoczęcia stresu zalewania. W pracy dyskutowana jest niezależność zmian parametrów fluorescencji chlorofilu a i indukowanego stresem zalewania zamykania się szparek.

Źródło:

Zeszyty Problemowe Postępów Nauk Rolniczych; 2002, 481, 1
0084-5477

Pojawia się w:

Zeszyty Problemowe Postępów Nauk Rolniczych

Dostawca treści:

Biblioteka Nauki

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Skocz do pozycji: 64.

Tytuł:

Fluorescence parameters of chlorophyll a halophytes as a response to salinity of post mining subsidence reservoirs

Autorzy:

Sierka, Edyta
Bujok, Michał
Stalmachova, Barbara
Horaczek, Tomasz

Powiązania:

https://bibliotekanauki.pl/articles/2203574.pdf

Data publikacji:

2022

Wydawca:

Instytut Technologiczno-Przyrodniczy

Tematy:

chlorophyll a fluorescence
climate change
halophyte
Najas marina
salinity

Opis:

The increasing salinity of water in reservoirs is caused by climate change. On the other hand, an increase in salinity promotes the group species, halophytes that tolerate or need NaCl for growth. The aim of this study was to identify the response of facultative halophytes’ photosynthetic apparatus efficiency (PE) to water salinity. The study covered the spiny water nymph (Najas marina L.) population in four mining subsidence reservoirs. Najas marina is a facultative halophyte which means that it can occur in both fresh and salt water. This plant has the characteristics of the species invasive, such as rapid biomass growth, and wide ecological tolerance. Water salinity, described by conductivity, in the reservoirs ranged from 646 to 3061 μS∙cm -1. PE was expressed in terms of chlorophyll a fluorescence parameters, which were collected in situ using a Pocket PEA device. Water parameters using a YSI ProDSS probe were identified. Data analysis was performed using OJIP test and s the non-parametric Spearman’s rank test (p ≤ 0.05). The relationship between chlorophyll a fluorescence parameters and water parameters showed that conductivity, salinity, water clarity, and nitrate content statistically significantly affected PE (p <0.05). Generally, the higher salinity e.g. more than 3000 μS∙m -1, supports PE of facultative halophyte at the stage of optimum development in the vegetation season.

Źródło:

Journal of Water and Land Development; 2022, Special Issue; 164--170
1429-7426
2083-4535

Pojawia się w:

Journal of Water and Land Development

Dostawca treści:

Biblioteka Nauki

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Skocz do pozycji: 65.

Tytuł:

The effect of selected plant extracts on the mitochondria of leukemic cancer cells in vitro
Wpływ wybranych wyciągów roślinnych na mitochondria białaczkowych komórek nowotworowych in vitro

Autorzy:

Tomečková, V.
Stupák, M.
Mojžišová, G.
Šutorová, M.
Mašlanková, J.

Powiązania:

https://bibliotekanauki.pl/articles/271488.pdf

Data publikacji:

2013

Wydawca:

Górnośląska Wyższa Szkoła Pedagogiczna im. Kardynała Augusta Hlonda

Tematy:

synchronous fluorescence fingerprint
fluorescence
mitochondria
arnika
hops
horse chestnut
cytotoxic effect
leukemic cells
fluorescencja
arnica
chmiel
kasztanowiec
efekt cytotoksyczny
białaczkowe komórki nowotworowe

Opis:

Individual plant extracts from horse chestnut, arnica and hops have been applied in vitro on Jurkat type leukemic cells from which mitochondria were isolated. Each sample of the isolated experimental mitochondria were monitored by the determination of proteins and by monitoring the fluorescence of endogenous fluorophores of experimental mitochondria compared with control mitochondria isolated from intact leukemic cells by means of the synchronous fluorescence fingerprint method. The horizontal cut of excitation-emission matrices at (λ = 240 nm) demonstrated the final graphical and mathematical fluorescence of mitochondria. The result is the presence of two fluorescent excitation-emission zones (the result of the overlapping effect of very similar fluorescence maxima values), which were subsequently compared with standard fluorophores. A reduction of protein content in all experimental mitochondria was observed, the most after the effect of arnica and the least after the influence of horse chestnut. The decrease of fluorescence of the first fluorescent zone was compared to control mitochondria. The decline of mitochondrial fluorescence confirmed the cytotoxic effect of the substances studied on the mitochondria in vitro.
Oceniono wpływ in vitro wyciągów wybranych roślin (kasztanowiec, chmiel, arnica) na mitochondria wyizolowane z nowotworowych komórek białaczkowych typu Jurkat. Każdą próbkę wyizolowanych eksperymentalnie mitochondriów monitorowano określając produkowane białka i oceniając fluorescencję endogennych fluoroforów z użyciem metody the synchronous fluorescence fingerprint i porównano z mitochondriami kontrolnymi uzyskanymi z komórek nie poddanych oddziaływaniu wyciągów. Odczyty wskazań emisji macierzy falą o długości (λ = 240 nm) ukazały fluorescencję mitochondriów. Stwierdzono dwie emisje, które porównano ze standardowymi fluorophorami. Zobrazowano redukcję zawartości białka we wszystkich doświadczalnych mitochondriach, największą po wyciągach z arnica i najmniejszą po kasztanowcu. Obniżenie fluorescencji pierwszej strefy było porównywalne z mitochondriami kontrolnymi. Obniżenie mitochondrialnej fluorescencji potwierdza cytotoksyczny efekt badanych substancji na mitochondia in vitro.

Źródło:

Journal of Ecology and Health; 2013, R. 17, nr 4, 4; 186-190
2082-2634

Pojawia się w:

Journal of Ecology and Health

Dostawca treści:

Biblioteka Nauki

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Skocz do pozycji: 66.

Tytuł:

Light-triggered protochlorophyllide to chlorophyllide reduction – new insights into enzyme-substrate interactions

Autorzy:

Gabruk, M.
Kruk, J.
Mysliwa-Kurdziel, B.

Powiązania:

https://bibliotekanauki.pl/articles/79867.pdf

Data publikacji:

2013

Wydawca:

Polska Akademia Nauk. Czytelnia Czasopism PAN

Tematy:

biosynthesis
chlorophyll
photosynthetic pigment
protochlorophyllide
chlorophyllide
enzyme substrate interaction
fluorescence

Źródło:

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2013, 94, 3
0860-7796

Pojawia się w:

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

Dostawca treści:

Biblioteka Nauki

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Skocz do pozycji: 67.

Tytuł:

Adoption of the time resolved fluorescence to oil type identification

Autorzy:

Baszanowska, E.
Otremba, Z.
Rohde, P.
Zieliński, O.

Powiązania:

https://bibliotekanauki.pl/articles/247122.pdf

Data publikacji:

2011

Wydawca:

Instytut Techniczny Wojsk Lotniczych

Tematy:

oil
fuels
lubricants
aquatic environment protection
time-resolved fluorescence
diagnostics

Opis:

The present development of a basic science, including physics of stimulated emission of light, opens up new diagnostic possibilities in relation to the quality of oils, including marine fuels and lubricants. In complicated chemical composition of oils both light absorbing compounds and compounds that emit light (fluorescent) are included. This causes the formation of complex structures in the spectral plots of fluorescence intensity as a function of wavelength of light exciting fluorescence and as a function of the wavelength of light emitted. Since the fluorescence excitation occurs in a short time of its disappearance. The shape of the fluorescence decay function depends on the chemical composition of oil, therefore, includes a mention of its kind, quality, level of degradation, and the like. In this paper exemplary charts of the intensity decay curves in different parts of the fluorescence spectra are shown (for light fuel). Presented charts are obtained using the experimental device constructed in Bremerhaven University of Applied Sciences (Germany) and are the attempt to use ability of oil substance to manifest its composition in the form of the fluorescence decay curve. An analysis of the chart shows that decay of fluorescence in this case is described by biexponential curve, therefore a kind of oil can be represented by two numerical values - two decay time constants. Operational use of this method will be possible only after a comparative study of different types and quality of oil in relation to the shape of the fluorescence decay curves.

Źródło:

Journal of KONES; 2011, 18, 2; 25-29
1231-4005
2354-0133

Pojawia się w:

Journal of KONES

Dostawca treści:

Biblioteka Nauki

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Skocz do pozycji: 68.

Tytuł:

Fluorescence Analysis as an Effective Method Used in Micro/Trace Explosive Detection

Autorzy:

Liu, Y.
Shu, Y.
Liu, Xue-yong.
Xiong, Y.
Zhong, Fa-chun.
Sun, Y.

Powiązania:

https://bibliotekanauki.pl/articles/358808.pdf

Data publikacji:

2009

Wydawca:

Sieć Badawcza Łukasiewicz - Instytut Przemysłu Organicznego

Tematy:

micro/trace explosive detection
fluorescence analysis
conjugated polymer
sensing materials

Opis:

There are many kinds of explosives, and their detection methods vary. Nitroaromatic compound is one of the composition of the explosives commonly used. The fuorescence will be quenched when they touch the fuorescent conjugated polymers. General methods for explosives detection have been summarized in this paper, in addition the application of novel fuorescence analysis technique in explosives detection has been introduced. Fluorescent conjugated polymer as chemical sensing material for explosive detection has been reviewed in detail, also a novel fuorescent sensing flm self-assembled by fuorescent small molecule pyrene and homogeneous fuoroimmunoassay have been presented briefy. The development of fuorescence analysis used in the area of determining explosives has been prospected.

Źródło:

Central European Journal of Energetic Materials; 2009, 6, 3-4; 303-311
1733-7178

Pojawia się w:

Central European Journal of Energetic Materials

Dostawca treści:

Biblioteka Nauki

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Skocz do pozycji: 69.

Tytuł:

Fluorescence spectra of oil after it contacts with aquatic environment

Autorzy:

Baszanowska, E.
Otremba, Z.
Toczek, H.
Rohde, P.

Powiązania:

https://bibliotekanauki.pl/articles/245054.pdf

Data publikacji:

2013

Wydawca:

Instytut Techniczny Wojsk Lotniczych

Tematy:

oil pollution
fluorescence spectroscopy
excitation-emission spectra
seawater
environment protection

Opis:

The main task for natural marine environment protection is to prevent the inflow of various contaminants including oil substances and the real challenge is the ability to rapidly detect these pollutants. Moreover important is to find the source or the maker of the oil spill. In this paper, we consider fundamental physical aspects in the area of possibility of monitoring the natural marine ecosystem based on fluorescence spectroscopy. We utilize the fluorescence ability of numerous oil components – mainly polycyclic hydrocarbons. The paper concerns the spectrofluorimetric characterization of oils have been in use during exploitation of the marine fleet, i.e. lubricate oil, fuels, transformer and hydraulic oils as well as crude oils or their residues. Every kind of oil has a chance to enter the marine environment, especially in a case of ship emergency or after collision with other vessel ore shoreline structure as well as when ship enters the stranding. After discharge of oil, some of oil components are dissolved in the water, bacteria or photochemical reactions, which results in transformation of composition of oil, degrade some. Fluorescence spectrometer Perkin Elmer LS55 was applied to obtain the fluorescence spectra using different excitation wavelength in the range from 240 nm to 500 nm. We discuss the changes of the shapes of excitationemission spectra (EEMs) of various types of oil and the EEMs spectra after contact of oil with seawater as an oil-inwater emulsion, which is the most frequent form of oil in the water column. Significant changes in the shape of spectra and displacement of the peaks are observed.

Źródło:

Journal of KONES; 2013, 20, 3; 29-34
1231-4005
2354-0133

Pojawia się w:

Journal of KONES

Dostawca treści:

Biblioteka Nauki

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Skocz do pozycji: 70.

Tytuł:

$K_{β}/K_{α}$ X-Ray Intensity Ratios for Cr, Mn, Fe, and Co Excited by 8.735 keV Energy

Autorzy:

Yılmaz, R.

Powiązania:

https://bibliotekanauki.pl/articles/1398200.pdf

Data publikacji:

2018-05

Wydawca:

Polska Akademia Nauk. Instytut Fizyki PAN

Tematy:

K X-ray intensity ratios
X-ray fluorescence
cross-sections

Opis:

In this study, the K shell intensity ratios $K_{β}/K_{α}$ have been experimentally determined for the elements Cr, Mn, Fe, and Co. Measurements have been carried out at 8.735 keV excitation energy by using secondary excitation method. K X-rays emitted by samples have been counted by a Si (Li) detector with 160 eV resolutions at 5.9 keV. The measured values were compared with the calculated theoretical values. The obtained values are in good agreement with the calculated values.

Źródło:

Acta Physica Polonica A; 2018, 133, 5; 1227-1230
0587-4246
1898-794X

Pojawia się w:

Acta Physica Polonica A

Dostawca treści:

Biblioteka Nauki

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Skocz do pozycji: 71.

Tytuł:

Effect of soil cultivation system on the efficiency of the photosynthetic apparatus in maize leaves (Zea mays L.)

Autorzy:

Stępień-Warda, Anna

Powiązania:

https://bibliotekanauki.pl/articles/2148090.pdf

Data publikacji:

2020-12-30

Wydawca:

Instytut Uprawy Nawożenia i Gleboznawstwa – Państwowy Instytut Badawczy

Tematy:

maize
chlorophyll fluorescence
direct seeding
monoculture
climate changes
reduced tillage

Opis:

The cultivation of maize in no-tillage farming systems has become increasingly important in recent years, due to the ob-served climate changes and the increasing droughts. Such a cul-tivation system has a positive effect on the physicochemical and biological properties of the soil, but above all allows for greater retention of water available to plants. The aim of the research was to assess the effect of the method of soil preparation for sowing maize grown in monoculture (plough, reduced, direct seeding) on the efficiency of photosystem II (PSII) in leaves and the yield of maize (Zea mays L.). The field experiment was carried out in 2017–2019 at the Agricultural Experimental Station IUNG-PIB in Grabów (Mazowieckie Voivodeship), using the split-block method. On three treatments, maize was grown in monocul-ture, and in a control treatment, in rotation. Three methods of preparing the soil for sowing in monoculture were used: full plough tillage, reduced tillage and direct seeding. The research showed, that the applied tillage system had a significant impact on the parameters of chlorophyll fluorescence and the yielding of maize, while the weather conditions, especially rainfall, were also of great importance. In conditions of water deficiency in the soil, maize cultivated in the reduced tillage and in direct seeding yielded better than in the crop rotation with full plough tillage. Also chlorophyll fluorescence indices (Fv/Fm and PI) in plants cultivated without the use of plough tillage showed higher values than in plants cultivated in rotation, which proves more effec-tive functioning of photosystem II in the conditions of no-tillage cultivation with limited rainfall and greater efficiency of the pho-tosynthetic apparatus under these conditions, which was reflected in the yielding of maize.

Źródło:

Polish Journal of Agronomy; 2020, 43; 57-62
2081-2787

Pojawia się w:

Polish Journal of Agronomy

Dostawca treści:

Biblioteka Nauki

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Skocz do pozycji: 72.

Tytuł:

Effect of biostimulants on chlorophyll fluorescence parameters of broccoli (Brassica oleracea var. italica) under drought stress and rewatering

Autorzy:

Kałużewicz, A.
Bączek-Kwinta
Krzesiński, W.
Spiżewski, T.
Zaworska, A.

Powiązania:

https://bibliotekanauki.pl/articles/11879855.pdf

Data publikacji:

2018

Wydawca:

Uniwersytet Przyrodniczy w Lublinie. Wydawnictwo Uniwersytetu Przyrodniczego w Lublinie

Tematy:

plant cultivation
broccoli
Brassica oleracea
chlorophyll fluorescence
biostimulant
drought stress

Opis:

The aim of the research was to determine the influence of biostimulants amino acids and amino acids + Ascophyllum nodosum filtrate on two broccoli cultivars ‘Agassi’ and ‘Tiburon’ and their response to soil drought. The plants were watered with Ascophyllum nodosum filtrate before planting and sprayed with amino acids after planting three times. Chlorophyll fluorescence measurements were performed before, during and after stress. They showed a considerable difference in cultivars’ response to stress, with ‘Agassi’ being more sensitive. Application of biostimulants enhanced the tolerance to drought stress. Maximum photochemical efficiency of PSII was unchanged, whereas the quantum yield of electron transport and photochemical fluorescence quenching values increased and the non-photochemical fluorescence quenching decreased. Moreover, the apparent photosynthetic electron transport rate rose. Chlorophyll content index was affected by the cultivar and application of biostimulants.

Źródło:

Acta Scientiarum Polonorum. Hortorum Cultus; 2018, 17, 1; 97-106
1644-0692

Pojawia się w:

Acta Scientiarum Polonorum. Hortorum Cultus

Dostawca treści:

Biblioteka Nauki

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Skocz do pozycji: 73.

Tytuł:

Ultraviolet-induced fluorescence of lubricate oils
Fluorescencja oleju smarnego wzbudzana światłem ultrafioletowym

Autorzy:

Baszanowska, E.
Otremba, Z.

Powiązania:

https://bibliotekanauki.pl/articles/134176.pdf

Data publikacji:

2015

Wydawca:

Polskie Towarzystwo Naukowe Silników Spalinowych

Tematy:

lubricate oil
fluorescence
optical fiber
olej smarny
fluorescencja
sensor optyczny

Opis:

The paper describes the study on relationship between the engine lubricate oil quality and fluorescence phenomenon. In order to determine the Excitation-Emission spectra (EEMs) of oil samples, a spectrofluorometer combined with an optical fiber system were applied. Two methods of fluorescence measurement: when oil diluted in non-fluorescent solvent (n-hexane) is placed in the quartz-cuvette inside the spectrofluorometer, and when oil sample is placed outside the spectrofluorometer (then EEMs is measured through the optical fiber sensor) were utilized to analyze the shape of EEMs of lubricate oil. Moreover in the second case of measurement, the optical fiber sensor was placed at an angle of 45 degrees to the oil surface, similarly above the oil surface and beneath the oil surface directly submerged in oil. Preliminary measurement results presented in this paper – on one hand – indicate diversity in the shape of EEMs depending on the method of fluorescence measurement, on the other hand – indicate possibility to measure fluorescence of oil directly in engine lubricate oil circuit.
W artykule przedstawiono wyniki badań związku pomiędzy jakością oleju smarnego a zjawiskiem fluorescencji. W celu wyznaczenia widm zupełnych fluorescencji próbek oleju wykorzystano spektrofluorymetr połączony ze światłowodowym systemem pomiarowym. Zanalizowano kształt widm odnoszących się do oleju smarnego w dwóch sytuacjach, gdy: olej jest rozpuszczony w niefluoryzującym medium (n-hexan) i umieszczony jest w przyrządzie w kwarcowej kuwecie oraz gdy próbka oleju znajduje się na zewnątrz przyrządu, sygnał jest przenoszony światłowodem. Ponadto przy pomiarach z zastosowaniem światłowodu ustawiona pod kątem 45° głowica pomiarowa zestawu światłowodowego znajduje się, odpowiednio: w powietrzu nad powierzchnią oleju albo pod powierzchnię oleju. Przedstawione w niniejszym artykule wstępne wyniki badań z jednej strony wskazują na zróżnicowanie kształtów widm zupełnych fluorescencji względem metody pomiarowej, z drugiej strony – wskazują na możliwość wyznaczania fluorescencji bez wstępnej obróbki laboratoryjnej, bezpośrednio w silnikowym obiegu oleju smarnego.

Źródło:

Combustion Engines; 2015, 54, 4; 21-25
2300-9896
2658-1442

Pojawia się w:

Combustion Engines

Dostawca treści:

Biblioteka Nauki

/5

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Skocz do pozycji: 74.

Tytuł:

Handheld ED-XRF spectrometers in geochemical investigation: Comparative studies for glacial deposits from Spitsbergen

Autorzy:

Kozak, Lidia
Silva Souza, Juliana
Nawrot, Adam
Proch, Jędrzej
Kaźmierski, Marcin
Zawieja, Agnieszka
Niedzielski, Przemysław

Powiązania:

https://bibliotekanauki.pl/articles/16538450.pdf

Data publikacji:

2021

Wydawca:

Polska Akademia Nauk. Czasopisma i Monografie PAN

Tematy:

Arctic
Svalbard
X-Ray fluorescence
metals
statistical analysis
spatial distribution

Opis:

This study presents the determination of the content of selected metals (Ba, Ca, Fe, Nb, Rb, Sr, Y, Zn, and Zr) in postglacial deposits from two glacial valleys (Ebbadalen and Elsadalen) in the Petunia Bay (southern Spitsbergen). The aim of the research was to experimentally check the usefulness of the handheld energy dispersive X-ray fluorescence technique in the study of samples from the polar zone, before performing the future field tests. Deposit analyses were performed (in parallel) with two handheld X-ray fluorescence spectrometers from different manufacturers, to investigate the accuracy and reliability of the instruments. The statistical analysis of the results indicated that the measurements carried out with two spectrometers were statistically significantly different, which was probably due to the different calibration characteristics used by the manufacturers. However, the analysis of the spatial distribution of element concentrations using Geographic Information System tools showed that the distribution maps of elements concentrations were similar regardless of the spectrometer used in the analyses.

Źródło:

Polish Polar Research; 2021, 42, 3; 163-172
0138-0338
2081-8262

Pojawia się w:

Polish Polar Research

Dostawca treści:

Biblioteka Nauki

/5

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Skocz do pozycji: 75.

Tytuł:

Interaction of anesthetic supplement thiopental with human serum albumin

Autorzy:

Khan, Shahper
Islam, Barira
Rajeswari, M
Usmani,, Hammad
Khan, Asad

Powiązania:

https://bibliotekanauki.pl/articles/1040762.pdf

Data publikacji:

2008

Wydawca:

Polskie Towarzystwo Biochemiczne

Tematy:

thiopental
fluorescence resonance energy transfer
thermodynamics
FT-IR
circular dichroism

Opis:

Thiopental (TPL) is a commonly used barbiturate anesthetic. Its binding with human serum albumin (HSA) was studied to explore the anesthetic-induced protein dysfunction. The basic binding interaction was studied by UV-absorption and fluorescence spectroscopy. An increase in the binding affinity (K) and in the number of binding sites (n) with the increasing albumin concentration was observed. The interaction was conformation-dependent and the highest for the F isomer of HSA, which implicates its slow elimination. The mode of binding was characterized using various thermodynamic parameters. Domain II of HSA was found to possess a high affinity binding site for TPL. The effect of micro-metal ions on the binding affinity was also investigated. The molecular distance, r, between donor (HSA) and acceptor (TPL) was estimated by fluorescence resonance energy transfer (FRET). Correlation between the stability of the TPL-N and TPL-F complexes and drug distribution is discussed. The structural changes in the protein investigated by circular dichroism (CD) and Fourier transform infrared (FT-IR) spectroscopy reflect perturbation of the albumin molecule and provide an explanation for the heterogeneity of action of this anesthetic.

Źródło:

Acta Biochimica Polonica; 2008, 55, 2; 399-409
0001-527X

Pojawia się w:

Acta Biochimica Polonica

Dostawca treści:

Biblioteka Nauki

/5

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Skocz do pozycji: 76.

Tytuł:

Photosynthetic activity in three vascular species of Spitsbergen vegetation during summer season in response to microclimate

Autorzy:

Barták, Miloš
Váczi Peter
Hájek, Josef

Powiązania:

https://bibliotekanauki.pl/articles/2051428.pdf

Data publikacji:

2012

Wydawca:

Polska Akademia Nauk. Czytelnia Czasopism PAN

Tematy:

Arctic
Svalbard
vascular plants
plant ecophysiology
net photosynthesis
chlorophyll fluorescence

Źródło:

Polish Polar Research; 2012, 4; 443-462
0138-0338
2081-8262

Pojawia się w:

Polish Polar Research

Dostawca treści:

Biblioteka Nauki

/5

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Skocz do pozycji: 77.

Tytuł:

Fluorescencja chlorofilu jako narzędzie do oceny stopnia eutrofizacji ekosystemów wodnych na przykładzie stawów na obszarze gminy Raszyn
Fluorescence of chlorophyll as a tool to assess the degree of eutrophication of aquatic ecosystems on the example of ponds in the area of Raszyn commune

Autorzy:

Szymański, N.
Burzyńska, I.
Kalaji, H. M.
Mastalerczuk, G.

Powiązania:

https://bibliotekanauki.pl/articles/400390.pdf

Data publikacji:

2018

Wydawca:

Polskie Towarzystwo Inżynierii Ekologicznej

Tematy:

eutrofizacja
fluorescencja chlorofilu
obszary wiejskie
eutrophication
chlorophyll fluorescence
rural areas

Opis:

Obecnie dostępne metody oceny stopnia eutrofizacji są kosztowne, czasochłonne i wymagają posiadania zaawansowanej aparatury laboratoryjnej oraz wiedzy. Celem niniejszej pracy było określenie prostej i uzasadnionej ekonomicznie metody monitorowania skutków eutrofizacji wody na obszarze rolniczym oraz powiązanie wyników monitorowania parametrów fizykochemicznych wody z fluorescencją chlorofilu jako potencjalnego zagrożenia dla zdrowia na podstawie ustalonych poziomów alarmowych. Badania prowadzono w zbiornikach wodnych zlokalizowanych na obszarze wiejskim, różniących się intensywnością eksploatowania terenów przyległych: znajdujących się w otoczeniu obszarów intensywnie uprawianych rolniczo oraz w obszarze chronionym rezerwatu przyrody. W pracy określono wydajność aparatu fotosyntetycznego glonów oraz właściwości fizykochemiczne wody. Badania wykazały, że wzrost intensywności fluorescencji chlorofilu związany jest ze zwiększonym ładunkiem substancji biogennych oraz ze wzmożonym wzrostem mikroogranizmow zawierających chlorofil. Wyniki wskazują, na możliwość zastosowana pomiarów fluorescencji chlorofilu do oceny stopnia eutrofizacji wód. Zaprezentowana metoda ma szanse na nieinwazyjną, szybką i precyzyjną ocenę stanu fizjologicznego ekosystemów wodnych na obszarach wiejskich, szczególnie narażonych na procesy eutrofizacji.
The currently available methods for the assessment of the eutrophication degree are expensive, time-consuming and require advanced laboratory equipment and knowledge. The aim of this work was to determine a simple and economically justified method of monitoring the effects of eutrophication of water in the agricultural area and linking the results of monitoring physicochemical parameters of water with chlorophyll fluorescence as a potential health risk on the basis of established alarm levels. The research was conducted in water reservoirs located in the rural area, differing in the intensity of exploitation of adjacent areas: located in the vicinity of intensively cultivated areas and in the protected area of the nature reserve. The work determined the efficiency of the photosynthetic apparatus of algae and physicochemical properties of water. The studies have shown that the increase in the chlorophyll fluorescence intensity is associated with an raised biogenic load and an increased occurrence of chlorophyll-containing micro-fractions. The results indicate the possibility of using chlorophyll fluorescence measurements to assess the degree of water eutrophication. The presented method can be used for a non-invasive, quick and precise assessment of the physiological status of water ecosystems in rural areas, particularly vulnerable to the eutrophication processes.

Źródło:

Inżynieria Ekologiczna; 2018, 19, 2; 73-80
2081-139X
2392-0629

Pojawia się w:

Inżynieria Ekologiczna

Dostawca treści:

Biblioteka Nauki

/5

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Skocz do pozycji: 78.

Tytuł:

Elevated strontium concentration in the blood of automobile workers in Kolkata

Autorzy:

Basu, R.
Mukhopadhyay, A.
Ray, S.S.
Chakrabarti, P.
Ram, S.S.
Sudarshan, M.
Chakrabarti, S.

Powiązania:

https://bibliotekanauki.pl/articles/1916666.pdf

Data publikacji:

2014

Wydawca:

Uniwersytet Medyczny w Białymstoku

Tematy:

Strontium
garage workers
blood
Energy Dispersive X-ray Fluorescence Technique

Opis:

Introduction: Though the trace element strontium’s (Sr) exact role in health remains unknown, at certain levels, it is toxic and, given the body’s low threshold for Sr, has been cited to cause nervous disorders. At different stages of the life cycle, organisms vary in their ability to discriminate Sr and calcium, which can cause age-related differences in gastrointestinal absorption. Though data regarding the effects of such absorption remain limited, it can reportedly impact health, the immune system, and chromosomal aberration. Purpose: To investigate and correlate levels of Sr concentration and any health problems at certain levels among automobile workers. Materials and methods: The blood of 41 automobile workers and 26 healthy controls of the Kolkata region was collected and whole blood Sr concentration estimated by the energy dispersive X-ray fluorescence (EDXRF) technique. Results: Automobile workers showed significantly (p <0.001) higher blood SR levels than controls from the same area, despite similar dietary habits and drinking-water quality. Conclusions: Automobile workers are exposed to different pollutants that can harm their health, and a trace element imbalance of Sr and its elevated concentrations in the blood may cause different health complications.

Źródło:

Progress in Health Sciences; 2014, 4, 2; 186-189
2083-1617

Pojawia się w:

Progress in Health Sciences

Dostawca treści:

Biblioteka Nauki

/5

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Skocz do pozycji: 79.

Tytuł:

Determination of heavy metals of road deposited sediment in Ado-Ekiti, Nigeria using XRF Technique

Autorzy:

Ogunmodede, O. T.
Ajayi, O. O.

Powiązania:

https://bibliotekanauki.pl/articles/411774.pdf

Data publikacji:

2014

Wydawca:

Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN

Tematy:

X –Ray fluorescence (XRF) technique
heavy metals
soil pollution
anthropogenic

Opis:

In this work x-ray fluorescence(XRF) technology was used to evaluate the soil pollution with heavy metals (K, Ti, Cr, Mn, Fe, Cu, Zr) in rain run-off deposited metal sediment of road side soil in Ado Ekiti, Nigeria. The investigated sediment of road side was collected in open places along the road at different districts in Ado Ekiti. XRF was carried out at the laboratory of Obafemi Awolowo University centre for energy research using handheld thermo scientific energy-dispersive XRF analyzer. The experimental result indicate that the concentration of heavy elements in Adebayo road is the highest level detected while the road at new Iyin road is lowest and they are greater than the level detected in a control soil collected from a zone situated far from the road. For the majority of metals, pronounced maximum, concentrations were detected in the site. Anthropogenic releases give rise to highest concentrations of the metals relative to the normal background values and in some locations their levels exceed the alert level admitted by the Nigeria guideline.

Źródło:

International Letters of Chemistry, Physics and Astronomy; 2014, 2; 36-40
2299-3843

Pojawia się w:

International Letters of Chemistry, Physics and Astronomy

Dostawca treści:

Biblioteka Nauki

/5

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Skocz do pozycji: 80.

Tytuł:

Exploring the Interactions Between Caffeic Acid and Human Serum Albumin Using Spectroscopic and Molecular Docking Techniques

Autorzy:

Jahanban-Esfahlan, Ali
Roufegarinejad, Leila
Tabibiazar, Mahnaz
Lorenzo, José M.
Amarowicz, Ryszard

Powiązania:

https://bibliotekanauki.pl/articles/1363286.pdf

Data publikacji:

2021

Wydawca:

Instytut Rozrodu Zwierząt i Badań Żywności Polskiej Akademii Nauk w Olsztynie

Tematy:

human serum albumin (HSA)
fluorescence
caffeic acid
interaction
molecular docking

Opis:

Ultraviolet-visible (UV–Vis) and fluorescence spectroscopy along with molecular docking were used to explore the interaction between human serum albumin (HSA) and caffeic acid (CA). CA is one of the major representatives of hydroxycinnamic acids in plants and is commonly present in plant-based foods. The mechanism by which CA quenched HSA fluorescence was determined to be static, and the values obtained for thermodynamic parameters indicated that the CA and HSA interaction was spontaneous. Hydrogen bonds and van der Waals forces were the main driving forces stabilizing the complex. The binding constant was in the order of 104/M and the number of binding sites for CA on HSA was calculated to be close to one. The results of fluorescence and UV–Vis spectroscopy showed that CA induced conformational changes in HSA structure. The distance of CA and the tryptophan residue of HSA, was determined to be ~2 nm by using Forster resonance energy transfer theory. The mode of binding and the binding site of CA on albumin were examined by performing molecular docking calculations. CA interacted with albumin in subdomain IA, and non–covalent interactions stabilized the complex. CA showed a high affinity for albumin, and thus this phenolic compound would be distributed in the body upon interacting with HSA.

Źródło:

Polish Journal of Food and Nutrition Sciences; 2021, 71, 1; 69-77
1230-0322
2083-6007

Pojawia się w:

Polish Journal of Food and Nutrition Sciences

Dostawca treści:

Biblioteka Nauki

/5

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Skocz do pozycji: 81.

Tytuł:

Unraveling protein-protein interactions using fluorescence imaging techniques

Autorzy:

Michalak, M.
Wojtaszek, P.

Powiązania:

https://bibliotekanauki.pl/articles/79933.pdf

Data publikacji:

2013

Wydawca:

Polska Akademia Nauk. Czytelnia Czasopism PAN

Tematy:

conference
protein-protein interaction
imaging technique
fluorescence
physiological process
living cell

Źródło:

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2013, 94, 3
0860-7796

Pojawia się w:

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

Dostawca treści:

Biblioteka Nauki

/5

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Skocz do pozycji: 82.

Tytuł:

Protochlorophyllide photoreduction in angiosperms: focus on protochlorophyllide – surroundings interaction in model system

Autorzy:

Mysliwa-Kurdziel, B.
Gabruk, M.
Kruk, J.
Strzalka, K.

Powiązania:

https://bibliotekanauki.pl/articles/79978.pdf

Data publikacji:

2013

Wydawca:

Polska Akademia Nauk. Czytelnia Czasopism PAN

Tematy:

conference
protochlorophyllide
angiosperm
chlorophyll
photosynthetic reaction
fluorescence intensity
imidazole
model system

Źródło:

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2013, 94, 3
0860-7796

Pojawia się w:

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

Dostawca treści:

Biblioteka Nauki

/5

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Skocz do pozycji: 83.

Tytuł:

A low ratio of red/far-red in the light spectrum accelerates senescence in nest leaves of Platycerium bifurcatum

Autorzy:

Oliwa, Jakub
Kornas, Andrzej
Skoczowski, Andrzej

Powiązania:

https://bibliotekanauki.pl/articles/952109.pdf

Data publikacji:

2017

Wydawca:

Polska Akademia Nauk. Czytelnia Czasopism PAN

Tematy:

hlorophyll a fluorescence
fern
nest leaves
photomorphogenesis
r/fr ratio
reflectance

Opis:

The fern Platycerium bifurcatum is a valuable component of the flora of tropical forests, where degradation of local ecosystems and changes in lighting conditions occur due to the increasing anthropogenic pressure. In ferns, phytochrome mechanism responsible for the response to changes in the value of R/FR differs from the mechanism observed in spermatophytes. This study analyzed the course of ontogenesis of nest leaves in P. bifurcatum at two values of the R/FR ratio, corresponding to shadow conditions (low R/FR) and intense insolation (high R/FR). The work used only non-destructive research analysis, such as measurements of reflectance of radiation from the leaves, their blue-green and red fluorescence, and the chlorophyll a fluorescence kinetics. This allowed tracing the development and aging processes in the same leaves. Nest leaves are characterized by short, intense growth and rapid senescence. The study identified four stages of development of the studied leaves related to morphological and anatomical structure and changing photochemical efficiency of PSII. Under the high R/FR ratio, the rate of ontogenesis of the leaf lamina was much slower than under the low R/FR value. As shown, the rapid aging of the leaves was correlated with faster decline of the chlorophyll content. It was shown that leaf senescence was accompanied by accumulation of polyphenols, anthocyanins and carotenoids on the basis of reflectance and fluorescence measurements in the blue-green range.

Źródło:

Acta Biologica Cracoviensia. Series Botanica; 2017, 59, 2
0001-5296

Pojawia się w:

Acta Biologica Cracoviensia. Series Botanica

Dostawca treści:

Biblioteka Nauki

/5

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Skocz do pozycji: 84.

Tytuł:

Salivary aldehyde dehydrogenase - temporal and population variability, correlations with drinking and smoking habits and activity towards aldehydes contained in food

Autorzy:

Giebułtowicz, Joanna
Dziadek, Marta
Wroczyński, Piotr
Woźnicka, Katarzyna
Wojno, Barbara
Pietrzak, Monika
Wierzchowski, Jacek

Powiązania:

https://bibliotekanauki.pl/articles/1040385.pdf

Data publikacji:

2010

Wydawca:

Polskie Towarzystwo Biochemiczne

Tematy:

superoxide dismutase
fluorescence
aldehyde dehydrogenase
aldehydes
nutrition safety
saliva
salivary peroxidase

Opis:

Fluorimetric method based on oxidation of the fluorogenic 6-methoxy-2-naphthaldehyde was applied to evaluate temporal and population variability of the specific activity of salivary aldehyde dehydrogenase (ALDH) and the degree of its inactivation in healthy human population. Analyzed was also its dependence on drinking and smoking habits, coffee consumption, and its sensitivity to N-acetylcysteine. Both the specific activity of salivary ALDH and the degree of its inactivation were highly variable during the day, with the highest activities recorded in the morning hours. The activities were also highly variable both intra- and interpersonally, and negatively correlated with age, and this correlation was stronger for the subgroup of volunteers declaring abstinence from alcohol and tobacco. Moderately positive correlations of salivary ALDH specific activity with alcohol consumption and tobacco smoking were also recorded (rs ~0.27; p=0.004 and rs =0.30; p=0.001, respectively). Moderate coffee consumption correlated positively with the inactivation of salivary ALDH, particularly in the subgroup of non-drinking and non-smoking volunteers. It was found that mechanical stimulation of the saliva flow increases the specific activity of salivary ALDH. The specific activity of the salivary ALDH was strongly and positively correlated with that of superoxide dismutase, and somewhat less with salivary peroxidase. The antioxidant-containing drug N-acetylcysteine increased activity of salivary ALDH presumably by preventing its inactivation in the oral cavity. Some food-related aldehydes, mainly cinnamic aldehyde and anisaldehyde, were excellent substrates of the salivary ALDH3A1 enzyme, while alkenals, particularly those with short chain, were characterized by lower affinity towards this enzyme but high catalytic constants. The protective role of salivary ALDH against aldehydes in food and those found in the cigarette smoke is discussed, as well as its participation in diminishing the effects of alcohol- and smoking-related oxidative stress.

Źródło:

Acta Biochimica Polonica; 2010, 57, 3; 361-368
0001-527X

Pojawia się w:

Acta Biochimica Polonica

Dostawca treści:

Biblioteka Nauki

/5

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Skocz do pozycji: 85.

Tytuł:

Trace elements in scalp hair of leukaemia patients

Autorzy:

Khuder, A.
Bakir, M. A.
Hasan, R.
Mohammad, A.
Habil, K.

Powiązania:

https://bibliotekanauki.pl/articles/971518.pdf

Data publikacji:

2014

Wydawca:

Instytut Chemii i Techniki Jądrowej

Tematy:

leukaemia
principal component analysis
scalp hair
U-test
X-ray fluorescence

Opis:

The aim of this study was to determine the concentration of Fe, Ni, Cu, Zn and Pb in scalp hair of leukaemia patients and healthy volunteers, using the optimised XRF method. Leukaemia hair samples were classifi ed corresponding to type, growth and age of the participants. The results showed that the studied trace elements (TEs) in both of leukaemia and control groups were positively skewed. In comparison with the control group, lower Fe, Cu, Zn and Pb and higher of Ni medians were found in all studied leukaemia patients. The median rank obtained by Mann–Whitney U-test revealed insignifi cant differences between the leukaemia patients subgroups and the controls. An exact probability (α < 0.05) associated with the U-test showed signifi cant differences between medians in leukaemia patients and controls groups for Pb (lymphatic/control, acute/control), Cu (lymphatic/control, chronic/control), Ni (lymphatic/control, chronic/control) and Fe (chronic/control). Very strong positive and negative correlations (r > 0.70) in the scalp hair of control group were observed between Ni/Fe-Ni, Cu/Fe-Cu, Zn/Fe-Zn, Pb/Fe-Pb, Cu/Ni-Zn/Ni, Cu/Ni-Pb/Ni, Zn/Ni-Pb/Ni, Zn/Fe-Zn/Cu, Pb/Ni-Ni and Ni/Fe-Pb/Ni, whereas only very strong positive ratios in the scalp hair of leukaemia patients group were observed between Ni/Fe-Ni, Cu/Fe-Cu, Zn/Fe-Zn and Pb/Fe-Pb, all correlations were signifi cant at p < 0.05. Other strong and signifi cant correlations were also observed in scalp hair of both groups. Signifi cant differences between grouping of studied TEs in all classifi ed leukaemia groups and controls were found using principal component analysis (PCA). The results of PCA confi rmed that the type and the growth of leukaemia factors were more important in element loading than the age factor.

Źródło:

Nukleonika; 2014, 59, 3; 111-120
0029-5922
1508-5791

Pojawia się w:

Nukleonika

Dostawca treści:

Biblioteka Nauki

/5

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Skocz do pozycji: 86.

Tytuł:

Indentification of three different chromosomal additions by chromosome painting using fluorescence in situ hybridization [FISH] technique

Autorzy:

Bocian, E
Stankiewicz, P
Stanczak, H
Obersztyn, E
Mazurczak, T

Powiązania:

https://bibliotekanauki.pl/articles/2047283.pdf

Data publikacji:

1996

Wydawca:

Polska Akademia Nauk. Czytelnia Czasopism PAN

Tematy:

chromosomal addition
fluorescence in situ hybridization method
hybridization
chromosome painting
cytogenetics

Opis:

Fluorescence in situ hybridization (FISH) is a very useful method for assessing chromosome rearrangements. When neither banding pattern nor clinical symptoms are sufficient to determine the origin of additional chromosomal fragment, FISH with multiple chromosome-specific libraries (chromosome painting), allows to solve this diagnostic problem rapidly. Three chromosomal additions, 7q+, 13p+ and 22q+, found in routine cytogenetic studies performed in children with phenotypic abnormalities were analysed using FISH. This technique documented the origin of the extra material to be derived from chromosome 16[der(7)t(7; 16)(q36.3;p 13.11)], 18[der(13)t(13; 18)(p12;q 12.2)] and 22[dup(22)(q11.2q13.1)], respectively. In two cases the abnormality arose de novo, while in the third case the product of translocation t(13;18) was maternal by origin. It was present in 30% of mother's lymphocytes, and in 70% of them a balanced Robertsonian translocation t(13q;15q) was found. In the presented cases the chromosome analysis with both traditional banding and chromosome painting techniques, allowed to establish final clinical diagnosis.

Źródło:

Journal of Applied Genetics; 1996, 37, 2; 197-204
1234-1983

Pojawia się w:

Journal of Applied Genetics

Dostawca treści:

Biblioteka Nauki

/5

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Skocz do pozycji: 87.

Tytuł:

Early detection of phosphorus deficiency stress in cucumber at the cellular level using chlorophyll fluorescence signals

Autorzy:

Sieczko, Leszek
Dąbrowski, Piotr
Kowalczyk, Katarzyna
Gajc-Wolska, Janina
Borucki, Wojciech
Janaszek-Mańkowska, Monika
Kowalczyk, Waldemar
Farci, Domenica
Kalaji, Hazem M.

Powiązania:

https://bibliotekanauki.pl/articles/2203572.pdf

Data publikacji:

2022

Wydawca:

Instytut Technologiczno-Przyrodniczy

Tematy:

abiotic stress
chlorophyll fluorescence
cucumber
JIP test
multidimensional statistical analysis
photosynthesis

Opis:

Abiotic stressors contribute to growth restriction and developmental disorders in plants. Early detection of the first signs of changes in plant functioning is very important. The objective of this study was to identify chlorophyll fluorescence parameters that change under phosphorus deficiency stress in cucumber. In this work, a trail to study the early changes caused by phosphorus deficiency in cucumber plants by analysing their photosynthetic performance is presented. Chlorophyll-a fluorescence (ChF) parameters were measured every 7 days for a period of 28 days. Measurements were made separately on young and old leaves and on cucumber fruit. Parameters that decreased during the stress were: p2G, PI abs, PI total, REo/CSo, and TRo/CSo. P deficiency decreased total electron carriers per RC (ECo/RC), yields (TRo/ABS (Fv/Fm), ETo/TRo, REo/ETo, ETo/ABS and REo/ABS), fluxes (REo/RC and REo/CSo) and fractional reduction of PSI end electron acceptors, and damaged all photochemical and non-photochemical redox reactions. Principal component analysis revealed a group of ChF parameters that may indicate early phosphorus deficiency in cucumber plants. Our results are used in the discovery of sensitive bioindicators of phosphorus deficiency in cucumber plants. Most JIP test parameters are linked to mathematical equations, so we recommend using of advanced statistical tools, such as principal component analysis, which should be considered very useful for stress identification. It has also been shown to be more effective in multivariate methods compared to univariate statistical methods was demonstrated.

Źródło:

Journal of Water and Land Development; 2022, Special Issue; 176--186
1429-7426
2083-4535

Pojawia się w:

Journal of Water and Land Development

Dostawca treści:

Biblioteka Nauki

/5

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Skocz do pozycji: 88.

Tytuł:

Badanie i analiza widm fluorescencyjnych do identyfikacji potencjalnych czynników zagrożenia biologicznego
Fluorescence spectra application and analysis for discrimination of potential biological warfare agents

Autorzy:

Kaliszewski, M.
Włodarski, M.
Kwaśny, M.
Młyńczak, J.
Kopczyński, K.
Trafny, E.
Szpakowska, M.
Mularczyk-Oliwa, M.
Bombalska, A.
Zawadzki, Z.
Mierczyk, Z.

Powiązania:

https://bibliotekanauki.pl/articles/210768.pdf

Data publikacji:

2010

Wydawca:

Wojskowa Akademia Techniczna im. Jarosława Dąbrowskiego

Tematy:

fluorescencja bakterii
aerozole biologiczne
autofluorescencja
bacteria fluorescence spectra
biological aerosols
autofluorescence

Opis:

W pracy zaprezentowano i porównano charakterystyki wzbudzeniowo-emisyjne aminokwasów, białek, bakterii, bakteriofaga MS2 oraz pyłków roślin. Pokazane zostały różnice pomiędzy drobnoustrojami hodowanymi i przygotowanymi w różnych warunkach. Rejestrowanie i analiza charakterystyk fluorescencyjnych możliwie licznej grupy związków i czynników biologicznych jest etapem niezbędnym do opracowania aparatury wykrywającej w czasie rzeczywistym chorobotwórcze drobnoustroje obecne w powietrzu.
The paper presents excitation-emmision maps of aminoacids, proteins, bacteria, bacteriophage MS2 and pollens. It shows differences between microorganisms grown and prepared in different conditions. The fluorescence data recorded in the study are necessary for the development of devices based on laser induced fluorescence that will be used for real time identification of airborne pathogenic microorganisms.

Źródło:

Biuletyn Wojskowej Akademii Technicznej; 2010, 59, 2; 363-374
1234-5865

Pojawia się w:

Biuletyn Wojskowej Akademii Technicznej

Dostawca treści:

Biblioteka Nauki

/5

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Skocz do pozycji: 89.

Tytuł:

Methods of peptide conformation studies.

Autorzy:

Biedrzyński, Andrzej

Powiązania:

https://bibliotekanauki.pl/articles/1044052.pdf

Data publikacji:

2001

Wydawca:

Polskie Towarzystwo Biochemiczne

Tematy:

fluorescence resonance energy transfer
NMR
circular dichroism
peptide conformation
hydrogen exchange

Opis:

In solution most of the peptides assume multiple flexible conformations. Determination of the dominant conformers and evaluation of their populations is the aim of peptide conformation studies, in which theoretical and experimental methods play complementary roles. Molecular dynamics or Monte Carlo methods are quite effective in searching the conformational space accessible to a peptide but they are not able to estimate, precisely enough, the populations of various conformations. Therefore, they must be supplemented by experimental data. In this paper, a short review of the experimental methods, most widely used in peptide conformational studies, is presented. Among them NMR plays the leading role. Valuable information is also obtained from hydrogen exchange, fluorescence resonance energy transfer, and circular dichroism measurements. The advantages and shortcomings of these methods are discussed.

Źródło:

Acta Biochimica Polonica; 2001, 48, 4; 1091-1099
0001-527X

Pojawia się w:

Acta Biochimica Polonica

Dostawca treści:

Biblioteka Nauki

/5

Artykuł

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Skocz do pozycji: 90.

Tytuł:

Non-invasive technique of crop heat-stress resistance estimation

Autorzy:

Trojak, Magdalena
Skowron, Ernest

Powiązania:

https://bibliotekanauki.pl/articles/1179185.pdf

Data publikacji:

2017

Wydawca:

Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN

Tematy:

barley
heat resistance
heat stress
modulated fluorescence (PAM)
photosystem II (PSII)

Opis:

Sessile organisms like plants have drastically reduce possibility to avoid harmful influence of the natural habitat. Evolution of first land plants allowed adapting to harsh conditions, yet despite broad range of tolerance factors such as excessive light and temperature impose limitation for biological activity because viability and in the case of crops the productivity is limited. In fact, physiologists make lots of efforts to increase stress tolerance searching and selecting new cultivars of known crops. As in most cases the exact mechanism of reached improvement is still missing selection is occupied by immense amount of time, energy and funds. Proposed in paper technique of stress resistance estimation is inexpensive and highly reproducible solution for crop selection even at early stage of development. It is based on well-known fluorescent assessment (PAM) of plant photosynthetic machinery expose to factor of interest. We tested two barley (Hordeum vulgare L.) cultivars spring Carina and winter Lomerit exposed to thermal stress and estimated maximal (Fv/Fm) and effective photosystem II quantum yield precisely specifying the way absorbed energy is utilized by photochemical (ΦPSII) or regulated (ΦNPQ) and non-regulated (ΦNO) non-photochemical manner. Studies have confirmed various heat-resistance of tested barley cultivars and proved PAM technique utility.

Źródło:

World Scientific News; 2017, 83; 168-181
2392-2192

Pojawia się w:

World Scientific News

Dostawca treści:

Biblioteka Nauki

/5

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Skocz do pozycji: 91.

Tytuł:

Estimation of mating-compatibility of the wild service tree [Sorbus torminalis [L.] Crantz] by observing pollen tube germination

Autorzy:

Wojciechowski, A
Bednorz, L.

Powiązania:

https://bibliotekanauki.pl/articles/2042041.pdf

Data publikacji:

2000

Wydawca:

Polska Akademia Nauk. Czytelnia Czasopism PAN

Tematy:

self-incompatibility
mating-compatibility
pollen tube
fluorescence
tree
Sorbus torminalis
germination

Źródło:

Journal of Applied Genetics; 2000, 41, 4; 253-258
1234-1983

Pojawia się w:

Journal of Applied Genetics

Dostawca treści:

Biblioteka Nauki

/5

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Skocz do pozycji: 92.

Tytuł:

Similarity of chromosome structure among Populus tremula var. davidiana, Populus alba and their hybrids revealed by FISH karyotype analysis

Autorzy:

Kim, Y.G.
Kwon, S.H.
Kang, H.I.
Yoem, D.B.
Kim, K.W.
Kim, H.H.
Kang, K.S.

Powiązania:

https://bibliotekanauki.pl/articles/2077655.pdf

Data publikacji:

2020

Wydawca:

Polska Akademia Nauk. Instytut Dendrologii PAN

Tematy:

Karyotype analysis
Fluorescence in situ hybridization
Ribosomal DNA site
Poplar
Cytogenetics

Opis:

The genus Populus is one of the important tree species in Korean peninsula and many other coun- tries in the world. It represents the model species of forest genomics because it grows fast and reproduces rapidly. In this reason, their genetic characteristics have been well studied and the whole genome has been sequenced completely in some species. However, cytogenetic study of the genus Populus has been limited. In the present study, karyotypes of Korean aspen (P. tremula var. davidiana), Silver poplar (P. alba) and their two hybrids, Suwon aspen (P. tremula var. glandulosa) and Hyun aspen (P. alba × P. tremula var. glandulsa) were analyzed by means of the fluorescence in situ hybridization (FISH). Root samples were collected from mature trees in the demonstration forest, located at Suwon, Kyonggi province in South Korea. The fresh root cells were examined by DAPI (4’,6-diamidino-2-phenylindole) staining and FISH using 45S rDNA and 5S rDNA probes. As the results, the chromosome compositions of all species were the same as 2n = 38. The karyotype formulas of Korean aspen, Silver poplar, Suwon aspen and Hyun aspen were 28m + 6sm + 4st (2sat), 26m + 10sm (2sat) + 2st, 26m + 12sm (2sat) and 28m + 10sm (2sat), respectively. The four species had one pair of 45S rDNA site and one pair of 5S rDNA site in common with FISH karyotypes. The similarity of FISH karyotypes among four species indicated close genetic relationship and coexistence of their interspecific hybrids. This research will provide genetic information on cytogenetic research of Populus and genetic mapping that can be applied to the breeding program of Populus in the near future.

Źródło:

Dendrobiology; 2020, 83; 68-74
1641-1307

Pojawia się w:

Dendrobiology

Dostawca treści:

Biblioteka Nauki

/5

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Skocz do pozycji: 93.

Tytuł:

Pochodne kumaryny do roli molekularnych sensorów fluorescencyjnych do zastosowań w naukach life science
Coumarin derivatives for the role of molecular fluorescent sensors for the application in life science

Autorzy:

Czarna, M.
Kamińska, I.
Ortyl, J.

Powiązania:

https://bibliotekanauki.pl/articles/134827.pdf

Data publikacji:

2015

Wydawca:

ADVSEO

Tematy:

kumaryna
sensory molekularne
fluoroscencja
coumarin
fluorescence
molecular sensor
molecular probes
fluorophores

Opis:

Fluorescent spectroscopy is an important method in life science which has recently gained popularity. Fluorescent spectroscopy is widely used for example by the researches in the variety of biological structures, intercellular interactions, various kinds of biomolecules and also for understanding of the biochemical processes which take place inside the living organisms. For this purpose, an application of fluorescent sensors and searching for more efficient and more sensitive fluorophore for illustrating, labelling and detection, have become a developing trend in biochemistry, medicine, biology and also in many other chemistry researches. Nowadays, issues which are connected to fluorescence are mainly applied to the newest achievements in the field of fluorescent methods and measurement techniques and also to the development and applications of fluorescent probes, what is closely showed up in this article.

Źródło:

Technical Issues; 2015, 4; 3-10
2392-3954

Pojawia się w:

Technical Issues

Dostawca treści:

Biblioteka Nauki

/5

Artykuł

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Skocz do pozycji: 94.

Tytuł:

Opracowanie parametrów generowania aerozoli bakteryjnych do badań przyrządem UVAPS
Determination of parameters of bacterial aerosol for measurement with UVAPS device

Autorzy:

Kaliszewski, M.
Włodarski, M.
Lewandowski, R.
Kwaśny, M.
Kopczyński, K.
Trafny, E. A.
Szpakowska, M.
Mularczyk-Oliwa, M.

Powiązania:

https://bibliotekanauki.pl/articles/210032.pdf

Data publikacji:

2010

Wydawca:

Wojskowa Akademia Techniczna im. Jarosława Dąbrowskiego

Tematy:

fluorescencja bakterii
aerozole biologiczne
autofluorescencja
bacteria fluorescence spectra
biological aerosols
autofluorescence

Opis:

W pracy przedstawione zostały wyniki wstępnych testów analizatora fluorescencji pojedynczych cząsteczek UVAPS 3314 firmy TSI. Celem pracy było testowanie unikalnej w skali kraju aparatury, określenie optymalnych dla pomiarów stężeń drobnoustrojów w aerozolu oraz metody generowania bioaerozoli. Badania wykazały, że wytwarzanie bioaerozolu z zawiesiny wodnej lub soli fizjologicznej w zamkniętej komorze powoduje powstawanie zbyt małego stężenia aerozolu bakterii w stosunku do całkowitej liczby cząstek. Efektem tego jest niemożność pomiaru fluorescencji przez przyrząd. Eksperymenty pozwoliły na wstępne oszacowanie możliwości pomiarowych UVAPS 3314.
The paper presents preliminary results of Ultraviolet Aerodynamic Particle Sizer's (UVAPS 3314, TSI) testing. The aim of work was testing the device that is probably unique in Poland, determination of concentration of microorganisms in aerosol optimal for measurements, and methodology of aerosol generation. Experiments showed that aerosol generated in closed chamber from water suspension or NaCl shows too low concentration of bacterial particles comparing to total amount of particles. As a result, it is not possible to record fluorescence signal of bacteria with UVAPS 3314 device. Performed experiments allowed for preliminary assessment of measurement capabilities of UVAPS.

Źródło:

Biuletyn Wojskowej Akademii Technicznej; 2010, 59, 3; 395-405
1234-5865

Pojawia się w:

Biuletyn Wojskowej Akademii Technicznej

Dostawca treści:

Biblioteka Nauki

/5

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Skocz do pozycji: 95.

Tytuł:

FISH method for identification of microbes in wastewater distribution systems

Autorzy:

Domańska, M.
Kuhn, R.
Łomotowski, J.
Stańczyk, E.

Powiązania:

https://bibliotekanauki.pl/articles/207711.pdf

Data publikacji:

2014

Wydawca:

Politechnika Wrocławska. Oficyna Wydawnicza Politechniki Wrocławskiej

Tematy:

bacteria
bioreactors
fluorescence microscopy
microorganisms
FISH method
fluorescence in situ hybridization
membrane bioreactor
bakteria
bioreaktory
mikroskopia fluorescencyjna
mikroorganizmy
metoda FISH
fluorescencyjna hybrydyzacja in situ
bioreaktor membranowy

Opis:

The fluorescence in situ hybridization (FISH) method is widely used to identify various types of cells. In comparison to cultivation dependent methods, identification of microbes by FISH is easier and generally takes several hours. A review of improvements to the FISH method has been presented, its advantages and disadvantages, as well as examples of application. Particular consideration was given to the efficiency of identification of microbes in samples taken from sewerage. The effectiveness of the method was confirmed by the results obtained in samples from the membrane bioreactor.

Źródło:

Environment Protection Engineering; 2014, 40, 3; 151-160
0324-8828

Pojawia się w:

Environment Protection Engineering

Dostawca treści:

Biblioteka Nauki

/5

Artykuł

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Skocz do pozycji: 96.

Tytuł:

Fluorescencyjne nanolipopolimersomy jako bezpieczne kontrasty w diagnostyce medycznej
Nanolipopolymersomes with a fluorescent wall as imaging agents in medical diagnostics

Autorzy:

Sierant, M.
Miksa, B.

Powiązania:

https://bibliotekanauki.pl/articles/278229.pdf

Data publikacji:

2014

Wydawca:

Sieć Badawcza Łukasiewicz - Instytut Inżynierii Materiałów Polimerowych i Barwników

Tematy:

diagnostyka medyczna
kontrast
nanolipopolimersomy
kapsułka polimerowa
kapsułka fluorescencyjna
sonda fluorescencyjna
sonda polimerowa
in vitro
medical diagnostics
imaging agent
nanolipopolymersomes
polymer capsule
fluorescence capsule
fluorescence probe
polymer probe

Opis:

Przedmiotem badań była synteza biokompatybilnych kapsułek polimerowych, które mogłyby spełniać rolę sondy fluorescencyjnej wykorzystywanej jako kontrasty w żywych komórkach. Otrzymaliśmy nanolipopolimersomy otoczone fluorescencyjną powłoką z usieciowanego polistyrenu z dodatkiem kumaryny 6. Wykazaliśmy na przykładzie barwników (Procion red, Procion blue, Lucifer yellow), że możliwa jest enkapsulacja hydrofilowych związków we wnętrzu trwałych, polimerowych pęcherzyków. Opracowana przez nas uniwersalna metoda syntezy stabilnych nanokapsułek wykorzystujących nietrwałe struktury liposomów może znaleźć szerokie zastosowanie w diagnostyce medycznej do transportu wielu substancji aktywnych oraz monitorowania ich lokalizacji w komórkach.
In our research we synthesized biocompatible probes for developing a stable macromolecule imaging system using nanolipopolymersomes in living cells. We prepared polymer vesicles with a fluorescent wall surrounded by on outer phospholipids shell suitable for dual detection (spectrophotometric and fluorescence). We developed highly fluorescent coumarinated poly(styrene-co-divinylbenzene) capsules with encapsulated hydrophilic dyes (Procion Red, Procion blue, Lucifer yellow). Our method of synthesis novel fluorescent hybrid materials can help in medical diagnostics, which can be uses in studies of the transport routs in living cells.

Źródło:

Przetwórstwo Tworzyw; 2014, [R.] 20, nr 1 (157), 1 (157); 88-91
1429-0472

Pojawia się w:

Przetwórstwo Tworzyw

Dostawca treści:

Biblioteka Nauki

/5

Artykuł

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Skocz do pozycji: 97.

Tytuł:

Synteza pochodnych hydroksy-2H-1-benzopiran-2-onu w polu promieniowania mikrofalowego oraz w warunkach ogrzewania konwencjonalnego
Synthesis of derivatives of hydroxy-2H-1-benzopyran-2-one using microwave-assisted and conventional heating methods

Autorzy:

Czarna, M.
Ortyl, J.

Powiązania:

https://bibliotekanauki.pl/articles/142463.pdf

Data publikacji:

2015

Wydawca:

Stowarzyszenie Inżynierów i Techników Przemysłu Chemicznego. Zakład Wydawniczy CHEMPRESS-SITPChem

Tematy:

pochodne hydroksykumaryny
kumaryna
promieniowanie mikrofalowe
fluorescencja
hydroxycoumarin derivatives
coumarin
microwave radiation
fluorescence

Opis:

W wyniku przeprowadzonych eksperymentów w polu promieniowania mikrofalowego oraz w warunkach ogrzewania konwencjonalnego, otrzymano szereg pochodnych hydroksy-2H-1-benzopiran- 2-onu o wysokim stopniu czystości. Wykonane badania spektroskopowe oraz analiza związków wykazały, że większość otrzymanych produktów charakteryzuje się interesującymi właściwościami spektroskopowymi, co pozwala na wykorzystanie ich do roli molekularnych sond fluorescencyjnych.
As a result of the conducted experiments under microwave radiation and under conditions of conventional heating, a number of high-purity derivatives of hydroxy-2H-1-benzopyran-2-one were obtained. The conducted spectroscopic measurements and compound analysis showed that the majority of obtained products exhibit interesting spectroscopic properties, which allows their use as a molecular fluorescence probes.

Źródło:

Chemik; 2015, 69, 5; 297-306
0009-2886

Pojawia się w:

Chemik

Dostawca treści:

Biblioteka Nauki

/5

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Skocz do pozycji: 98.

Tytuł:

Oil identification based on total synchronous spectra

Autorzy:

Baszanowska, E.
Otremba, Z.

Powiązania:

https://bibliotekanauki.pl/articles/246342.pdf

Data publikacji:

2014

Wydawca:

Instytut Techniczny Wojsk Lotniczych

Tematy:

fluorescence spectroscopy
total synchronous spectra
lubricate oil
vessel engine room
marine environment

Opis:

Contribution of marine carriage in the global transport is in permanent developing. It leads to increase the unintentional oil spills, which may induce serious disturbances in functioning of the natural marine ecosystem, especially when it interferes with other technical activities in the marine space. That fact contributes to increase the interest in searching of new research techniques to protect the natural ecosystem. Therefore, the main task of marine international organisations concentrates on the effective and rapid detection of oil spill and on the possibility of identifying oil pollutants as well as on origin of pollution. The purpose of the study is to characterise the oil belonging to the vessel engine lubricate oils. We analyse the stage results of investigations based on one of types of fluorescence spectroscopy, namely: synchronous spectra. To characterise the oil, the Aqualog Horiba spectrofluorometer was applied, which allows performing precise measurement in a short time. Based on the measured excitation-emission spectra, total synchronous fluorescence spectra for oil were obtained using various wavelength intervals. Total synchronous fluorescence spectra of petroleum substances allow finding wavelength interval typical for particular type of oil. This approach could allow obtaining of complex mixtures, such as oils, more efficient description. We discuss the total synchronous fluorescence spectra for engine oil (Marinol type) dissolved in n-hexane in various concentrations of oil as a possible tool proposed to oil type identification. We present changes and variation of the total synchronous fluorescence spectra for oil with various oil concentrations.

Źródło:

Journal of KONES; 2014, 21, 2; 15-20
1231-4005
2354-0133

Pojawia się w:

Journal of KONES

Dostawca treści:

Biblioteka Nauki

/5

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Skocz do pozycji: 99.

Tytuł:

Properties of the Baltic crude oil in the oil-in-water emulsion form : excitation-emission spectra

Autorzy:

Baszanowska, E.
Otremba, Z.

Powiązania:

https://bibliotekanauki.pl/articles/246400.pdf

Data publikacji:

2018

Wydawca:

Instytut Techniczny Wojsk Lotniczych

Tematy:

crude oil
excitation-emission spectra
fluorescence
seawater
ropa naftowa
fluorescencja
woda morska

Opis:

Due to the fact that marine environment contamination by oil substances are not uncommon, it is necessary to improve the ability of determine their origin. Therefore, research on the possibility of recognizing the type of oil using its individual characteristic manifested in the processes of fluorescence excitation has been undertaken. Oil pollutants present in seawater mainly coming from ship drives, tankers, pipelines or sea bottom seeps, moreover oil leaks from offshore extraction equipment also are possible. For this reason, the crude oil extracted from the Baltic Sea deposit in the Polish Maritime Areas has been chosen for tests. Fluorescence properties of water containing small amounts of oil (concentrations from 5.59 to 55.4 ppm) in the form of oil-in-water emulsion were tested. Individual samples were assigned matrices of fluorescent values for different wavelengths, while excited by monochromatic light also for different wavelengths. The obtained matrices were visualized as a contour maps and 3D charts. For considered concentrations of crude oil dispersed in water, the wavelength-independent fluorescence maximum was determined. Obtained result indicates that in the studied wavelength range, the total fluorescence intensity is proportional to the oil concentration only for the lowest oil concentrations. The analysis of the objective parameter of the difference between the shapes of spectra indicates the similarity of the spectral shape for the lowest oil concentrations. These results are the methodological suggestion, that for the purpose of identifying the type of oil dispersed in water, spectra should be determined for sequences of different dilutions, until the excitation-emission spectra shapes become independent from the oil concentration.

Źródło:

Journal of KONES; 2018, 25, 1; 7-14
1231-4005
2354-0133

Pojawia się w:

Journal of KONES

Dostawca treści:

Biblioteka Nauki

/5

Artykuł

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Skocz do pozycji: 100.

Tytuł:

Wykorzystanie techniki fluoroscencji rentgenowskiej (XRF) do szybkiej analizy pierwiastkowej biomasy

Autorzy:

Kubik, W.

Powiązania:

https://bibliotekanauki.pl/articles/274379.pdf

Data publikacji:

2017

Wydawca:

Roble

Tematy:

fluorescencja rentgenowska
XRF
biomasa
analiza pierwiastkowa
X-ray fluorescence
biomass
elemental analysis

Źródło:

LAB Laboratoria, Aparatura, Badania; 2017, 22, 1; 30-34
1427-5619

Pojawia się w:

LAB Laboratoria, Aparatura, Badania

Dostawca treści:

Biblioteka Nauki

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