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Wyświetlanie 1-15 z 267
Tytuł:
Testing of the synchronous fluorescence in application to used lubricate oil characterisation
Autorzy:
Baszanowska, E.
Otremba, Z.
Powiązania:
https://bibliotekanauki.pl/articles/243560.pdf
Data publikacji:
2016
Wydawca:
Instytut Techniczny Wojsk Lotniczych
Tematy:
marine fleet exploitation
lubricate oil
fluorescence spectroscopy
synchronous fluorescence spectra
Opis:
Reliability of the ship propulsion system depends, inter alia, on the quality of lubricate oils. Research indicates that exploitive features of oil are reduced with it runtime in the ship engine circuit. At the present the basic parameters representing exploitive features of oil are: water content, total acid number and total base number or viscosity vs. temperature, anti-wear performance, further: content of additives and their concentrations, reaction products, and other contaminating substances, also magnetically separated large ferromagnetic particles, the size, the shape, the composition and concentration of the abnormal wear particles. Additional properties of oil are described based on atomic emission spectroscopy, granulometry of separated from the lubricant sample by magnets and gravity, permeability changes, as well as rheometric and tribometric characteristics. Complexity of composition of lubricate oil raises the idea to use changes of relative content of any compounds for characterization of oil exploitive features. Fluorescence method is possible to use for oil properties characterisation due to the presence mono and polycyclic compounds in oil that induces fluorescence phenomenon. Therefore identification the fluorescence spectra when exploitation time of oil increases could be novel indicator to monitor of lubricate oil exploitive quality. In this study we present changes of oil fluorescence expressed by synchronous spectra, taking into account working time of exemplary lubricate oil in the ship engine.
Źródło:
Journal of KONES; 2016, 23, 2; 39-44
1231-4005
2354-0133
Pojawia się w:
Journal of KONES
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Spectroscopic methods in application to oil pollution detection in the sea
Autorzy:
Baszanowska, E.
Otremba, Z.
Powiązania:
https://bibliotekanauki.pl/articles/248141.pdf
Data publikacji:
2012
Wydawca:
Instytut Techniczny Wojsk Lotniczych
Tematy:
marine transport
oil pollution
spectroscopic methods
fluorescence
time-resolved fluorescence
Opis:
The escalating maritime transport as well as offshore crude oil exploitation and pipeline transportation leads to the increase of feasibility of the discharge of petroleum substances to the sea environment events. Therefore, it is necessary to rapidly detect of oil pollution to minimize the extent of contamination in the seawater. The aim of this study is to discuss the assumptions of the optical methods using for detection of oil pollution in the seawater. We consider the different approaches of oil-in-water detection using spectral active and passive techniques with emphasis on fluorescence techniques. The intensive development of scientific apparatus and methods in light spectroscopy leads to build various airborne or underwater specialized devices (UV-, IR-scanners, fluorosensors), which allows to detect oil pollution in relatively large areas of the sea. The possibility of measurements of oil pollution in the seawater is particularly important for monitoring, especially in very difficult weather conditions and highly waved sea surface. Such possibilities should be given by devices for in-situ measurements based on time-resolved fluorescence. Such measurements allow obtaining the significant information about presence of oil in really short time. As we show in this study, the wide-range development of fluorescence techniques opens the diagnostic opportunity not only for detection of the oil-in-water content but also for identification the type of oil directly in seawater or even determination of the source of oil pollution.
Źródło:
Journal of KONES; 2012, 19, 1; 15-20
1231-4005
2354-0133
Pojawia się w:
Journal of KONES
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
O nieznanej naturze meteorytów
On the unknown nature of meteorites
Autorzy:
Kosina, Romuald
Powiązania:
https://bibliotekanauki.pl/articles/1033006.pdf
Data publikacji:
2014
Wydawca:
Polskie Towarzystwo Meteorytowe
Tematy:
UV fluorescence
meteorites
variation
Opis:
The variable UV-fluorescence of minerals is well known, and this is attributed to some defects in crystallographic structure or some admixtures, e.g. REE elements. So far, UV-fluorescence has not been applied to describe the nature of meteorites. The plates of forty-five meteorites were examined in three simultaneous emission spectra under an epi-fluorescence microscope. Meteorites were characterized quantitatively according to twelve colours of fluorescence, arbitrarily identified. A numerical method of non-metric multidimensional scaling was used to put colour characteristics as well as the meteorites into an ordination space. Each colour characteristic carries its own information independent of the others. NWA 4039, Bilanga, Gujba and NWA 5437 are extremes in the ordination space. Sahara 560 and NWA 791 expressed very poor and very rich fluorescence, respectively. UV-fluorescence can be a good screening method to quickly distinguish space minerals with crystallographic defects and/or those which are contaminated by REE elements. In subsequent research, other more advanced methods will have to be used.
Źródło:
Acta Societatis Metheoriticae Polonorum; 2014, 5; 72-80
2080-5497
Pojawia się w:
Acta Societatis Metheoriticae Polonorum
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
In vitro fluorescence studies of transcription factor IIB-DNA interaction
Autorzy:
Górecki, Andrzej
Figiel, Małgorzata
Dziedzicka-Wasylewska, Marta
Powiązania:
https://bibliotekanauki.pl/articles/1038975.pdf
Data publikacji:
2015
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
transcription factors
TF2B
TFIIB
fluorescence spectroscopy
fluorescence quenching
site directed mutagenesis
Opis:
General transcription factor TFIIB is one of the basal constituents of the preinitiation complex of eukaryotic RNA polymerase II, acting as a bridge between the preinitiation complex and the polymerase, and binding promoter DNA in an asymmetric manner, thereby defining the direction of the transcription. Methods of fluorescence spectroscopy together with circular dichroism spectroscopy were used to observe conformational changes in the structure of recombinant human TFIIB after binding to specific DNA sequence. To facilitate the exploration of the structural changes, several site-directed mutations have been introduced altering the fluorescence properties of the protein. Our observations showed that binding of specific DNA sequences changed the protein structure and dynamics, and TFIIB may exist in two conformational states, which can be described by a different microenvironment of W52. Fluorescence studies using both intrinsic and exogenous fluorophores showed that these changes significantly depended on the recognition sequence and concerned various regions of the protein, including those interacting with other transcription factors and RNA polymerase II. DNA binding can cause rearrangements in regions of proteins interacting with the polymerase in a manner dependent on the recognized sequences, and therefore, influence the gene expression.
Źródło:
Acta Biochimica Polonica; 2015, 62, 3; 413-421
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
A coumarin-based fluorescent probe for specific and rapid detection of fluoride ions
Autorzy:
Xin, Z.-H.
Meng, Y.-L.
Wu, Y.-H.
Feng, Z.-K.
Kang, Y.-F.
Powiązania:
https://bibliotekanauki.pl/articles/778907.pdf
Data publikacji:
2018
Wydawca:
Zachodniopomorski Uniwersytet Technologiczny w Szczecinie. Wydawnictwo Uczelniane ZUT w Szczecinie
Tematy:
Fluorion
Fluorescence
Coumarin
Probe
Bioimaging
Opis:
A simple and coumarin-based fluorescence probe has been designed and synthesized with silyl group as recognition group of fluoride ions (F−) in this study. The results showed that the fluorescence intensity of the probe displayed prominent enhancement with addition of F− at 445 nm with incubation of 1 min. There was an excellent linear relationship between fluorescence intensity and fluoride concentration from 0 to 30 μM (0~0.57 ppm), which offered the important condition for the quantitative analysis. In addition, the highly selective response to fluorion, the low detection limit with 28 nM (0.532 ppb), low toxicity and bioimaging afforded an advantage for practical application and detecting fluoride in biological systerms.
Źródło:
Polish Journal of Chemical Technology; 2018, 20, 4; 1-5
1509-8117
1899-4741
Pojawia się w:
Polish Journal of Chemical Technology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Future prospects for investigating ciliate biodiversity
Autorzy:
Rajter, Ľubomír
Lu, Borong
Rassoshanska, Erika
Dunthorn, Micah
Powiązania:
https://bibliotekanauki.pl/articles/52000785.pdf
Data publikacji:
2022
Wydawca:
Uniwersytet Jagielloński. Wydawnictwo Uniwersytetu Jagiellońskiego
Tematy:
Biodiversity
Ciliophora
fluorescence
staining methods
Opis:
Ciliates have a long history of being central in evolutionary and ecological studies on eukaryotic microorganisms. Although thousands of species have been discovered, their total diversity still remains unknown. Here, we will discuss two unsolved problems that hinder the further exploration of ciliate diversity at the species level, and potential solutions to these problems are proposed. First, ciliate morphospecies are difficult to identify because the different silver stains are not scalable (they do not represent high-throughput methods) and basic supplies are lacking (e.g., protargol); a solution may be the development of fluorescent staining techniques. Second, ciliate phylogenetic species are difficult to identify because of extensive paralogy in nuclear-protein-coding genes; a solution may be to concentrate on sequencing mitochondrial genomes. These two approaches could be integrated into a high-throughput fluorescent-single-cell sorting and mitochondrial genomes sequencing process that would enable the observation and better understanding of ciliate species on a massive scale.
Źródło:
Acta Protozoologica; 2022, 61; 35-46
0065-1583
1689-0027
Pojawia się w:
Acta Protozoologica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Obrazowanie struktury chondrytu Kuźnica w mikroskopie konfokalnym
Imaging the structure of the Kuźnica chondrite with a confocal microscope
Autorzy:
Paterczyk, Bohdan
Woźniak, Marek
Powiązania:
https://bibliotekanauki.pl/articles/30146481.pdf
Data publikacji:
2024-04
Wydawca:
Polskie Towarzystwo Meteorytowe
Tematy:
meteorite
Kuźnica
confocal microscopy
fluorescence
Opis:
Confocal microscopy is commonly used in mineralogy for structural analysis and to investigate the chemical composition of minerals. In our work we performed imaging of structural defects in meteorites i.e. imaging of microstructures, inclusions, fractures and cracks. Information obtained in this way enables better understanding of minerals' origin and evolution. The aim of our work was not concentrated on the deep description of physical processes related to confocal microscope (CSLM) imaging. This paper is limited to the presentation of a few CSLM functions and possibilities, which have practical application in the interpretation of fluorescence microscope imaging of minerals. Confocal microscopy enables identification and preview of localization of selected minerals and therefore can be used to image of the distribution of chemical compounds in minerals.
Źródło:
Acta Societatis Metheoriticae Polonorum; 2024, 15; 96-115
2080-5497
Pojawia się w:
Acta Societatis Metheoriticae Polonorum
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Antiproliferative effect of β-escin - an in vitro study
Autorzy:
Mojžišová, Gabriela
Kello, Martin
Pilátová, Martina
Tomečková, Vladimíra
Vašková, Janka
Vaško, Ladislav
Bernátová, Silvia
Mirossay, Ladislav
Mojžiš, Ján
Powiązania:
https://bibliotekanauki.pl/articles/1038845.pdf
Data publikacji:
2016
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
escin
apoptosis
mitochondria
fluorescence fingerprint
Opis:
This study examined the antiproliferative effects of β-escin (E) in cancer cells. The study showed that E inhibited cancer cells growth in a dose-dependent manner. The flow cytometric analysis revealed an escin-induced increase in the sub-G1 DNA content, which is considered to be a marker of apoptosis. Apoptosis was also confirmed by annexin V staining and DNA fragmentation assay. These effects were associated with increased generation of reactive oxygen species (ROS), caspase-3 activation and decreased mitochondrial membrane potential (MMP). Moreover, escin decreased mitochondrial protein content and mitochondrial fluorescence intensity as well as caused depletion of glutathione (GSH). However, activity of glutathione peroxidase (GPx) and glutathione reductase (GR) was not significantly changed in escin-treated cells. In conclusion, our results demonstrated that E has apoptotic effects in human cancer cells through the mechanisms involving mitochondrial perturbation. Although the exact mechanism needs to be investigated further, it can be concluded that E may be a useful candidate agent for cancer treatment.
Źródło:
Acta Biochimica Polonica; 2016, 63, 1; 79-87
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Zastosowanie techniki stroboskopowej do pomiarów czasów życia fluorescencji materiałów biologicznych. Cz. 1, Podstawy metody, aparatura i metodyka pomiarów
Application of stroboscopic technique for measurement of biological materials fluorescence lifetimes. Pt. 1, Basics of stroboscopic technique, instruments and methods
Autorzy:
Włodarski, M.
Kwaśny, M.
Kaliszewski, M.
Rutecka, B.
Powiązania:
https://bibliotekanauki.pl/articles/209313.pdf
Data publikacji:
2009
Wydawca:
Wojskowa Akademia Techniczna im. Jarosława Dąbrowskiego
Tematy:
czas życia fluorescencji
metoda stroboskopowa
fluorescencja białek
UV LED
fluorescence lifetime
stroboscopic method
protein fluorescence
Opis:
W pracy przedstawiono podstawy stroboskopowej metody pomiarów czasów życia fluorescencji, aparaturę, opracowaną metodykę pomiarów i analizę procesu dekonwolucji sygnałów. Pokazano, że krzywe zaniku fluorescencji z wykorzystaniem źródeł światła typu LED UV, (280 i 340 nm) mogą znaleźć zastosowanie do charakterystyki aminokwasów, białek, bakterii - w tym symulantów i interferentów bojowych środków biologicznych. Podstawowymi zaletami metody są: jej szybkość, możliwość wykorzystania tanich źródeł wzbudzenia, wysoka czułość, prostota obsługi, modułowa budowa umożliwiająca szybką wymianę źródeł wzbudzenia. Badania przeprowadzone na standardowych wzorcach - NATA, NADH wykazały zgodność czasów życia fluorescencji z danymi literaturowymi.
Stroboscopic method for determination of fluorescence lifetimes, using 280 nm and 340 nm UV LED sources, may be used for characterization of biological substances - including simulants and interferents of Biological Warfare Agents (BWA). Main advantages of this method are: quickness of measurement, cheap UV sources, high sensitivity, simplicity of operation, modular construction allowing quick changing of sources. Principle of stroboscopic method, developed method of measurements and analysis of deconvolution process are presented in this work. Results of measurement of NATA and NADH standards correspond to data published by other authors.
Źródło:
Biuletyn Wojskowej Akademii Technicznej; 2009, 58, 4; 165-176
1234-5865
Pojawia się w:
Biuletyn Wojskowej Akademii Technicznej
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Synthesis and Characterization of an Efficient New Liquid Laser Dye Material - Chalcone (DMAPPP)
Autorzy:
Elzupir, A.
Ibnaouf, K.
Idriss, H.
Ibrahem, M.
Prasad, S.
Alrajhi, M.
AlSalhi, M.
Alaamer, A.
Powiązania:
https://bibliotekanauki.pl/articles/1030569.pdf
Data publikacji:
2018-01
Wydawca:
Polska Akademia Nauk. Instytut Fizyki PAN
Tematy:
chalcone
DMAPPP
solvents effect
fluorescence
ASE
Opis:
This paper comprises the synthesis and characterization of 3-(4-(dimethylamino)phenyl)-1-phenyl-(2E)-propen-1-one (DMAPPP) and its application as a new laser medium. The absorption and fluorescence spectra of DMAPPP under different solvents and concentrations have been investigated. The amplified spontaneous emission performance of DMAPPP under various concentrations, organic solvents and pump pulse energies of Nd:YAG laser (355 nm) was also studied. The amplified spontaneous emission spectra of DMAPPP in solution were compared with a conventional laser dye of coumarin 503, under the same identical conditions. The gain and the fluorescence quantum yield of DMAPPP were determined. The most important features are: (1) DMAPPP has an excellent photochemical stability, (2) the amplified spontaneous emission from the DMAPPP was tuned in the wavelength region between 515 and 548 nm. This could be the first detailed paper on laser properties of DMAPPP.
Źródło:
Acta Physica Polonica A; 2018, 133, 1; 121-125
0587-4246
1898-794X
Pojawia się w:
Acta Physica Polonica A
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Aktywowana termicznie opóźniona fluorescencja (TADF) – jako rozwiązanie problemu niskich wydajności układów fluorescencyjnych OLED
Thermally activated delayed fluorescence – as a solution to the low yield problem of the fluorescent OLEDs
Autorzy:
Pander, P.
Powiązania:
https://bibliotekanauki.pl/articles/1215145.pdf
Data publikacji:
2016
Wydawca:
Stowarzyszenie Inżynierów i Techników Przemysłu Chemicznego. Zakład Wydawniczy CHEMPRESS-SITPChem
Tematy:
OLED
TADF
opóźniona fluorescencja
delayed fluorescence
Opis:
Rozwój technologii wyświetlania obrazu doprowadził do opracowania ekranów typu OLED, których użycie jest coraz powszechne. Współcześnie dąży się do poprawy kwantowej wydajności (EQE) OLED. Organiczne diody elektroluminescencyjne zawsze cierpiały z powodu ograniczonej wydajności kwantowej, co jest wywołane zdolnością wykorzystania zaledwie 25% stanów wzbudzonych. TADF, czyli aktywowana termicznie opóźniona fluorescencja, umożliwia użycie do 100% stanów wzbudzonych powstających w diodzie, a zatem zmniejszeniu ulega udział bezemisyjnej relaksacji cząsteczek. Zjawisko to występuje dla cząsteczek o małej całce nakładania HOMO i LUMO, co skutkuje małą przerwą energetyczną singlet-tryplet.
The development of display technology led to invention of OLED screens whose use is becoming more common nowadays. Today one of the goals is to improve OLED external quantum efficiency (EQE). Organic light-emitting diodes have always suffered from limited quantum efficiency due to harvesting of only 25% of excited states. TADF, which is thermally activated delayed fluorescence, allows to harvest up to 100% of excited states formed in the OLED, thus to decrease a contribution of the non-radiative relaxation. This phenomenon occurs for molecules possessing a small integral overlap of HOMO and LUMO, which results in a small singlet-triplet energy gap.
Źródło:
Chemik; 2016, 70, 6; 318-325
0009-2886
Pojawia się w:
Chemik
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Predicting emission spectra of fluorescent materials from their absorbance spectra using the artificial neural network
Autorzy:
Shams-Nateri, A
Piri, N
Powiązania:
https://bibliotekanauki.pl/articles/173176.pdf
Data publikacji:
2015
Wydawca:
Politechnika Wrocławska. Oficyna Wydawnicza Politechniki Wrocławskiej
Tematy:
fluorescence
prediction
absorbance
emission
neural networks
Opis:
Artificial neural networks have been shown to be able to approximate any continuous nonlinear functions and have been used to build data based empirical models for nonlinear processes. This work studies primarily the performance of neural networks as a tool for predicting the emission spectra of fluorescent materials from their absorbance, and further, tends to the determination of the optimal topology of the neural network for this purpose. In order to do this, spectral data were initially analyzed by a principal component analysis technique. The first four principal components were used as input nodes of neural networks with various training algorithms – namely cascade- and feed-forward algorithms – and also, various numbers of hidden layers and nodes. The obtained results indicate that the RMS error in a testing data set decreased with increasing the number of neurons and the minimal network architecture for a data prediction problem consists of two hidden layers, respectively with 9 and 1 nodes for both neural networks. Additionally, a better performance was obtained with the cascade-forward neural network, especially in a small number of nodes. The obtained results indicate that the neural networks can be used to provide a relationship between the absorbance as an input and the emission as a target.
Źródło:
Optica Applicata; 2015, 45, 4; 545-557
0078-5466
1899-7015
Pojawia się w:
Optica Applicata
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Uptake of photofrin II, a photosensitizer used in photodynamic therapy, by tumour cells in vitro
Autorzy:
Chwiłkowska, Agnieszka
Saczko, Jolanta
Modrzycka, Teresa
Marcinkowska, Anna
Malarska, Anna
Bielewicz, Julita
Patalas, Dorota
Banaś, Teresa
Powiązania:
https://bibliotekanauki.pl/articles/1043629.pdf
Data publikacji:
2003
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
photofrin II
photodynamic therapy
fluorescence spectra
Opis:
Photosensitizing dyes are used in fluorescence diagnostics and photodynamic therapy (PDT). These usually hematoporphyrin derivatives (HpD) accumulate preferentially within neoplastic tissues. HpD is a mixture of ether and ester linked porphyrins. Its partially purified form is known under the commercial name of photofrin II (PII). PII emission spectra were studied in a hydrophilic (PBS) and a lipophilic (PC liposomes) environment. Red shift was observed in their emission maxima from 615 nm in buffer solution to 635 nm in lipid. Identical red shift was obtained when the intracellular fluorescence of two cancer cell lines, MCF 7 and Jurkat, incubated with PII was investigated. Thus, intramembrane localization of PII may be suggested. As determined from the total fluorescence intensity, the uptake of PII was only slightly higher for Jurkat than for MCF 7 cells. Nevertheless the kinetics of the uptake was found to be different for both cell lines.
Źródło:
Acta Biochimica Polonica; 2003, 50, 2; 509-513
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The phenomenon of fluorescence in immunosensors
Autorzy:
Kłos-Witkowska, Aleksandra
Powiązania:
https://bibliotekanauki.pl/articles/1038798.pdf
Data publikacji:
2016
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
biosensor
immunosensor
fluorescence
absorbance
UV/Vis
Opis:
The phenomenon of fluorescence in immunosensors is described in this paper. Both structure and characteristics of biosensors and immunosensors are presented. Types of immunosensors and the response of bioreceptor layers to the reaction with analytes as well as measurements of electrochemical, piezoelectric and optical parameters in immunosensors are also presented. In addition, detection techniques used in studies of optical immunosensors based on light-matter interactions (absorbance, reflectance, dispersion, emission) such as: UV/VIS spectroscopy, reflectometric interference spectroscopy (RIfs), surface plasmon resonance (SPR), optical waveguide light-mode spectroscopy (OWLS), fluorescence spectroscopy. The phenomenon of fluorescence in immunosensors and standard configurations of immunoreactions between an antigen and an antibody (direct, competitive, sandwich, displacement) is described. Fluorescence parameters taken into account in analyses and fluorescence detection techniques used in research of immunosensors are presented. Examples of immunosensor applications are given.
Źródło:
Acta Biochimica Polonica; 2016, 63, 2; 215-221
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Spectroscopic Methods Studies in Polymer
Autorzy:
Rahi, Saad Khalid
Hassan, Ehssan Salah
Abd, Ahmed N.
Powiązania:
https://bibliotekanauki.pl/articles/1190136.pdf
Data publikacji:
2016
Wydawca:
Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
Tematy:
ESCA
polymer
Fluorescence spectra
Excitation wavelength
Opis:
The physico – chemical changes induced in polymers following to radiation can be studied by a range of spectroscopic techniques. Recent developments in instrumentation and data analysis procedures in electronic, vibrational and magnetic resonance spectroscopes have provided considerable new insights, into polymer structure and behavior. The applications of these spectroscopic methods in polymer studies are reviewed with emphasis on their utility in investigations of radiation effects on macromolecules
Źródło:
World Scientific News; 2016, 42; 41-53
2392-2192
Pojawia się w:
World Scientific News
Dostawca treści:
Biblioteka Nauki
Artykuł
Wyświetlanie 1-15 z 267

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