Skocz do pozycji: 1.

Tytuł:

Adaptation of PCR technique for quantitative estimation of genetic material from different regions of chromosome 21 in cases of trisomy 21.

Autorzy:

Nowacka, Joanna
Helszer, Zofia
Walter, Zofia
Płucienniczak, Andrzej
Kałużewski, Bogdan

Powiązania:

https://bibliotekanauki.pl/articles/1041513.pdf

Data publikacji:

2004

Wydawca:

Polskie Towarzystwo Biochemiczne

Tematy:

quantitative polymerase chain reaction
trisomy 21

Opis:

Pre- and postnatal diagnosis of chromosomal aberrations is generally based on conventional cytogenetic analysis. In this paper, we have devised a quantitative polymerase chain reaction (Q-PCR) method to determine gene dose effects and applied it in cases of regular trisomy 21 as a model. The method is based on quantitative assessment of PCR products after using primers amplifying DNA fragments located in the pericentromeric, heterochromatic, euchromatic and telomeric regions of chromosome 21. A gene dose effect on the amount of PCR product in cases of trisomy 21 was confirmed. Moreover, a correlation between the amount of the PCR product of the examined sequences and their location in the chromosome was observed. The obtained results suggest that the Q-PCR technique can be applied in the diagnosis of aneuploidies.

Źródło:

Acta Biochimica Polonica; 2004, 51, 4; 995-1001
0001-527X

Pojawia się w:

Acta Biochimica Polonica

Dostawca treści:

Biblioteka Nauki

/5

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Skocz do pozycji: 2.

Tytuł:

Improved method of isolation of total nucleic acids from hop plants and grapevine before the RT-PCR by addition of polyvinylpolypyrrolidone
Usprawniona metoda izolacji calkowitych kwasow nukleinowych z chmielu i winorosli przez RT-PCR przez dodanie poliwinylopolipirolidonu

Autorzy:

Cajza, M
Folkman, W.

Powiązania:

https://bibliotekanauki.pl/articles/65582.pdf

Data publikacji:

2003

Wydawca:

Polska Akademia Nauk. Czytelnia Czasopism PAN

Tematy:

isolation
total nucleic acid
nucleic acid
hop plant
grapevine
RT-PCR method zob.reverse transcription polymerase chain reaction
addition
polyvinylpolypyrrolidone
reverse transcription polymerase chain reaction
polymerase chain reaction

Źródło:

Journal of Plant Protection Research; 2003, 43, 4; 375-380
1427-4345

Pojawia się w:

Journal of Plant Protection Research

Dostawca treści:

Biblioteka Nauki

/5

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Skocz do pozycji: 3.

Tytuł:

Application of polymerase chain reaction-restriction fragment length polymorphism (RFLP-PCR) in the analysis of single nucleotide polymorphisms (SNPs)

Autorzy:

Tarach, Piotr

Powiązania:

https://bibliotekanauki.pl/articles/1830648.pdf

Data publikacji:

2021-09-29

Wydawca:

Uniwersytet Łódzki. Wydawnictwo Uniwersytetu Łódzkiego

Tematy:

nucleotide polymorphisms
DNA analysis
polymerase chain reaction

Opis:

Polymerase chain reaction-restriction fragment length polymorphism (RFLP-PCR) is a technique used to identify single nucleotide polymorphisms (SNPs) based on the recognition of restriction sites by restriction enzymes. RFLP-PCR is an easy-to-perform and inexpensive tool for initial analysis of SNPs potentially associated with some monogenic diseases, as well as in genotyping, genetic mapping, lineage screening, forensics and ancient DNA analysis. The RFLP-PCR method employs four steps: (1) isolation of genetic material and PCR; (2) restriction digestion of amplicons; (3) electrophoresis of digested fragments; and (4) visualisation. Despite its obsolescence and the presence of high-throughput DNA analysis techniques, it is still applied in the analysis of SNPs associated with disease entities and in the analysis of genetic variation of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). RFLP-PCR is a low-cost and low-throughput research method allowing for the analysis of SNPs in the absence of specialised equipment, and it is useful when there is a limited budget.

Źródło:

Acta Universitatis Lodziensis. Folia Biologica et Oecologica; 2021, 17; 48-53
1730-2366
2083-8484

Pojawia się w:

Acta Universitatis Lodziensis. Folia Biologica et Oecologica

Dostawca treści:

Biblioteka Nauki

/5

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Skocz do pozycji: 4.

Tytuł:

Polymorphism in Syringa rDNA regions assessed by PCR technique

Autorzy:

Smolik, M.
Andrys, D.
Franas, A.
Krupa-Malkiewicz, M.
Malinowska, K.

Powiązania:

https://bibliotekanauki.pl/articles/41641.pdf

Data publikacji:

2010

Wydawca:

Polska Akademia Nauk. Instytut Dendrologii PAN

Tematy:

polymorphism
lilac
Syringa
rDNA region
polymerase chain reaction

Opis:

The Syringa genus is characterizedby a multiplicity of forms. Its chief asset is the ornamental value of thousands of accessions, species or hybrids. From a phylogenetic point of view the genus is difficult in an explicit classification due to its frequently complex genome. The aim of this study was to determine the possibility for the identification of genotypic diversity and genetic relationships in the nrDNA sequence of some selected Syringa accessions – part of a collection of the Dendrological Garden in Przelewice (Poland). For this purpose, the PCR technique together with a combination of various ‘universal’ primers designed for the nrDNA sequence analysis were employed. Fourteen Syringa accessions: Syringa × chinensis Willd., S. × prestoniae Mc Kelv., S. × prestoniae ‘Telimena’, S. × prestoniae ‘Jaga’, S. × prestoniae ‘Basia’, S. meyeri ‘Palibin’, S. vulgaris ‘Miss Ellen Willmott’, S. vulgaris, S. vulgaris ‘Jules Simon’, S. vulgaris ‘Katherine Havemeyer’, S. vulgaris ‘Krasawica Moskvy’, S. vulgaris ‘Mirabeau’, S. vulgaris ‘Madame Lemoine’ and S. vulgaris ‘Niebo Moskvy’ made up the research material. In the conducted amplifications, genetic profiles were obtained for 14 combinations among the 25 combinations of different pairs of primers used. The nrDNA templates coding the small subunit (SSU), 5.8S subunit andITS1, ITS2 andIGS sequences were amplified. In PCR reactions a total of 33 PCR products were generated, of which 21 (64%) products were polymorphic, 6 (18%) monomorphic and6 (18%) were genotype-specific. For the lilac accessions examined246 amplicons were generated from ~230 to ~1100 bp in length. The analysis of both the dendrogram and the genetic similarity matrix revealedlow diversity between the examinedaccessions. For most they rangedfrom 70 to 80%, andthe greatest diversity (87%) was foundbetween the S. × prestoniae: ‘Basia’ and‘Telimena’ accessions, while the lowest (57%) was observed between S. vulgaris ‘Katherine Havermeyer’ and S. × chinensis.

Źródło:

Dendrobiology; 2010, 64
1641-1307

Pojawia się w:

Dendrobiology

Dostawca treści:

Biblioteka Nauki

/5

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Skocz do pozycji: 5.

Tytuł:

Molecular diagnostics of Sarcocystis spp. infections

Autorzy:

Stojecki, K.
Karamon, J.
Sroka, J.
Cencek, T.

Powiązania:

https://bibliotekanauki.pl/articles/2088002.pdf

Data publikacji:

2012

Wydawca:

Polska Akademia Nauk. Czytelnia Czasopism PAN

Tematy:

molecular diagnostics
Sarcocystis
infection
sarcocystosis
polymerase chain reaction

Opis:

Protozoa of the genus Sarcocystis (phylum Apicomplexa, family Sarcocystidae) is one of the most common parasites affecting animals. Interspecies diagnostic of Sarcocystis genus was based on electron microscopy for many years. Because of absence of visible differences between species with reachable magnifications, light microscopy is useless. In many cases serological diagnostic method have lack of sensitivity. A variety of molecular methods have been developed and used to detect and identify Sarcocystis spp. and to assess the genetic diversity among this protozoan from different population/hosts. Nowadays, molecular diagnostic is the common, time/cost effective method used all over the world to interspecies differentiation.

Źródło:

Polish Journal of Veterinary Sciences; 2012, 15, 3
1505-1773

Pojawia się w:

Polish Journal of Veterinary Sciences

Dostawca treści:

Biblioteka Nauki

/5

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Skocz do pozycji: 6.

Tytuł:

Advanced methods of bacteriological identification in a clinical microbiology laboratory

Autorzy:

Zukowska, M.E.

Powiązania:

https://bibliotekanauki.pl/articles/2098275.pdf

Data publikacji:

2020

Wydawca:

Instytut Medycyny Wsi

Tematy:

clinical microbiology
molecular method
modern method
multiplex polymerase chain reaction
real-time polymerase chain reaction
next generation sequencing
MALDI-TOF mass spectrometry

Opis:

Introduction and objective. Conventional, culture-based methods of bacterial identification and drug-susceptibility testing are considered the gold standard in medical microbiology. In recent years, classical microbiological methods have been supplemented with modern analytical and molecular methods. The aim of the review was to discusses the methods which have been permanently adapted to bacteriological microbiological diagnostics. Abbreviated description of the state of knowledge. Currently, PCR, as well as other nucleic acid amplification tests and sequencing techniques, are part of the standard repertoire of microbiological diagnostics. With regard to the quality and speed of pathogen identification, the introduction of mass spectrometry techniques into routine microbiological diagnostics work-up has been revolutionary. Within a short time in many laboratories, Matrix-Assisted Laser Desorption/Ionisation – Time of Flight Mass Spectrometry (MALDI TOF MS) systems have almost completely replaced conventional biochemical pathogen identification. Conclusions. Microbiological diagnostics is an indispensable element of a targeted therapy. The techniques used in the laboratory depend primarily on the laboratory’s apparatus, the costs of the analysis, as well as the sensitivity and specificity of a method. However, regardless of the culture-based methods universality, advanced techniques have permanently established themselves in diagnostics. Confident information about the detected organism and treatment possibilities in a combination with the clinical context are conducive to successful therapy. Although modern methods still require validation and close collaboration between clinicians, microbiologists and bioinformaticians, these methods, once deemed to be the future, have already arrived.

Źródło:

Journal of Pre-Clinical and Clinical Research; 2021, 15, 2; 68-72
1898-2395

Pojawia się w:

Journal of Pre-Clinical and Clinical Research

Dostawca treści:

Biblioteka Nauki

/5

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Skocz do pozycji: 7.

Tytuł:

Antimicrobial activities and phylogenetic study of Erythrina senegalensis DC (Fabaceae) seed lectin

Autorzy:

Enoma, Samuel
Adewole, Taiwo S.
Agunbiade, Titilayo O.
Kuku, Adenike

Powiązania:

https://bibliotekanauki.pl/articles/16672367.pdf

Data publikacji:

2023

Wydawca:

Polska Akademia Nauk. Czasopisma i Monografie PAN

Tematy:

antimicrobial
Erythrina senegalensis
lectin
phylogenetic analysis
polymerase chain reaction

Opis:

Erythrina senegalensis (Fabaceae) have been traditionally used in the treatment of microbial ailments, and the specific agent mediating its efficacy has been investigated in several studies. In this study, the antimicrobial activity of purified E. senegalensis lectin (ESL) was analyzed. The phylogenetic relationship of the gene encoding lectin with other legume lectins was also established to investigate their evolutionary relationship via comparative genomics. Antimicrobial activity of ESL against selected pathogenic bacteria and fungi isolates was evaluated by the agar well diffusion method, using fluconazole (1 mg/ml) and streptomycin (1 mg/ml) as positive controls for fungi and bacteria sensitivity, respectively. Potent antimicrobial activity of ESL against Erwinia carotovora, Pseudomonas aeruginosa, Klebsiella pneumonia, Staphylococcus aureus, Aspergillus niger, Penicillium camemberti, and Scopulariopsis brevicaulis was observed, with inhibition zones ranging from 18 to 24 mm. Minimum inhibitory concentrations of ESL ranged between 50 and 400 μg/ml. Primer-directed polymerase chain reaction of E. senegalensis genomic DNA detected a 465-bp lectin gene with an open reading frame encoding a 134-amino acid polypeptide. The obtained nucleotide sequence of the ESL gene shared high sequence homology: 100, 100, and 98.18% with Erythrina crista-galli, Erythrina corallodendron, and Erythrina variegata lectin genes, respectively, suggesting that the divergence of Erythrina lectins might follow species evolution. This study concluded that ESL could be used to develop lectin-based antimicrobials, which could find applications in the agricultural and health sectors.

Źródło:

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2023, 104, 1; 21-32
0860-7796

Pojawia się w:

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

Dostawca treści:

Biblioteka Nauki

/5

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Skocz do pozycji: 8.

Tytuł:

DNA isolation from Cryptosporidium oocysts directly from stool samples for diagnostic goals using PCR

Autorzy:

Sulima, P.

Powiązania:

https://bibliotekanauki.pl/articles/840154.pdf

Data publikacji:

1998

Wydawca:

Polskie Towarzystwo Parazytologiczne

Tematy:

diagnostic goal
stool
polymerase chain reaction
Cryptosporidium
DNA
oocyst

Źródło:

Annals of Parasitology; 1998, 44, 3
0043-5163

Pojawia się w:

Annals of Parasitology

Dostawca treści:

Biblioteka Nauki

/5

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Skocz do pozycji: 9.

Tytuł:

Detection of bovine leucocyte adhesion deficiency [BLAD] carriers using a new PCR test

Autorzy:

Kaminski, S
Czarnik, U

Powiązania:

https://bibliotekanauki.pl/articles/2046599.pdf

Data publikacji:

1997

Wydawca:

Polska Akademia Nauk. Czytelnia Czasopism PAN

Tematy:

cattle
bovine leucocyte adhesion deficiency
polymerase chain reaction
bacterial infection

Opis:

In this report we demonstrate a simple, effective and reliable diagnostic test of BLAD carrier detection based on specific PCR amplification of a 367 bp CD18 gene fragment and RFLP analysis using Taq I restriction enzyme. In a non-random population of 220 animals we found 48 BLAD carriers. Within the amplified PCR fragment an unknown intron sequence of 159 bp was identified.

Źródło:

Journal of Applied Genetics; 1997, 38, 1; 51-55
1234-1983

Pojawia się w:

Journal of Applied Genetics

Dostawca treści:

Biblioteka Nauki

/5

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Skocz do pozycji: 10.

Tytuł:

Vegetable oil plant wastewater treatment by bacterial isolates : a study in the city of Hila, Iraq

Autorzy:

Salim, Hanan Kareem
Al-Ahmed, Suad Ghali Kadhim

Powiązania:

https://bibliotekanauki.pl/articles/2048496.pdf

Data publikacji:

2021

Wydawca:

Instytut Technologiczno-Przyrodniczy

Tematy:

biotreatment
polymerase chain reaction
PCR
sewage
vegetable oil plant
wastewater

Opis:

The present study was to reflect the use of some bacteria in the treatment and removal of pollutants in three selected wastewater sites, including a vegetable oil plant (viz. Al-Etihad Food Industries), the main wastewater treatment station in the city of Hila, and Al-Hila River water from October 2019 to January 2020. The bacterial isolates identified in these three sites were Klebsiella pneumoniae, Escherichia coli, Enterobacteria cloacae, Pseudomonas aeruginosa, Thalasobacillus devorans, Acinetobacter baumannii, and Bacillus subtilis. The molecular study of the bacterial isolates involved the detection of bacterial genera using the polymerase chain reaction (PCR). The results showed that water had a variable nature, depending on the substances in it. It recorded varying chemical and physical property values, ranging between 6.36 and 7.82 for pH and from 2500 to 7100 mg∙dm-3 for total alkalinity. Additional values were 713–2051 μS∙cm-1 for electrical conductivity (EC), 5.90–9.80 mg∙dm-3 for chemical oxygen demand (COD), and 480–960 mg∙dm-3 for total hardness. The given values were also 0.20–0.65 μg∙dm-3, 0.03-0.23 μg∙dm-3, and 0–107 mg∙dm-3 for nitrite (NO2), phosphate (PO4) oils, respectively.

Źródło:

Journal of Water and Land Development; 2021, 51; 163-167
1429-7426
2083-4535

Pojawia się w:

Journal of Water and Land Development

Dostawca treści:

Biblioteka Nauki

/5

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Skocz do pozycji: 11.

Tytuł:

Hantavirus RNA not detected in Ixodes ricinus ticks

Autorzy:

Wojcik-Fatla, A.
Zajac, V.
Knap, J.P.
Dutkiewicz, J.

Powiązania:

https://bibliotekanauki.pl/articles/49890.pdf

Data publikacji:

2011

Wydawca:

Instytut Medycyny Wsi

Tematy:

hantavirus
RNA
Ixodes ricinus
tick
epidemiology
polymerase chain reaction
Polska

Źródło:

Annals of Agricultural and Environmental Medicine; 2011, 18, 2
1232-1966

Pojawia się w:

Annals of Agricultural and Environmental Medicine

Dostawca treści:

Biblioteka Nauki

/5

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Skocz do pozycji: 12.

Tytuł:

Isolation, Identification and Preservation of Pectinolytic Bacteria Pathogenic to Potato

Autorzy:

Lebecka, Renata

Powiązania:

https://bibliotekanauki.pl/articles/2199695.pdf

Data publikacji:

2017-06-20

Wydawca:

Instytut Hodowli i Aklimatyzacji Roślin

Tematy:

Blackleg
Dickeya
Pectobacterium
Polymerase Chain Reaction
selective medium
soft rot

Opis:

Blackleg of potato plants and soft rot of tubers are caused by several species of pectinolytic bacte-ria from genera Pectobacterium and Dickeya. The text describes simple methods of isolating bacteria from symptomatic and symptomless organs of potato plants, their identification using Polymerase Chain Reaction (PCR) and preservation.

Źródło:

Plant Breeding and Seed Science; 2017, 75; 87-96
1429-3862
2083-599X

Pojawia się w:

Plant Breeding and Seed Science

Dostawca treści:

Biblioteka Nauki

/5

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Skocz do pozycji: 13.

Tytuł:

The influence of LDHA gene polymorphism on relative level of its expression in racing pigeons
Polimorfizm w genie LDHA i jego wpływ na względny poziom ekspresji u gołębi pocztowych

Autorzy:

Jędrzejczak-Silicka, M.
Yu, Y.-H.
Cheng, Y.-H.
Dybus, A.

Powiązania:

https://bibliotekanauki.pl/articles/2606361.pdf

Data publikacji:

2018

Wydawca:

Zachodniopomorski Uniwersytet Technologiczny w Szczecinie. Wydawnictwo Uczelniane ZUT w Szczecinie

Tematy:

gene polymorphism
gene expression
pigeon
real-time polymerase chain reaction

Źródło:

Acta Scientiarum Polonorum. Zootechnica; 2018, 17, 3; 9-15
1644-0714

Pojawia się w:

Acta Scientiarum Polonorum. Zootechnica

Dostawca treści:

Biblioteka Nauki

/5

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Skocz do pozycji: 14.

Tytuł:

SYBR Green-based real-time polymerase chain reaction assay for detection of porcine parvovirus 6 in pigs

Autorzy:

Sun, P.
Bai, C.X.
Zhang, D.
Wang, J.
Yang, K.K.
Cheng, B.Z.
Li, Y.D.
Wang, Y.

Powiązania:

https://bibliotekanauki.pl/articles/2087328.pdf

Data publikacji:

2020

Wydawca:

Polska Akademia Nauk. Czytelnia Czasopism PAN

Tematy:

porcine parvovirus 6
real-time polymerase chain reaction
SYBR Green

Źródło:

Polish Journal of Veterinary Sciences; 2020, 23, 2; 197-202
1505-1773

Pojawia się w:

Polish Journal of Veterinary Sciences

Dostawca treści:

Biblioteka Nauki

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Skocz do pozycji: 15.

Tytuł:

Quantification of thioredoxin mRNA expression in the rat hippocampus by real-time PCR following oxidative stress.

Autorzy:

Yalcin, Ayfer

Powiązania:

https://bibliotekanauki.pl/articles/1041524.pdf

Data publikacji:

2004

Wydawca:

Polskie Towarzystwo Biochemiczne

Tematy:

kainic acid
real-time polymerase chain reaction
oxidative stress
thioredoxin

Opis:

Thioredoxin (Trx) is a multifunctional protein with a redox-active disulfide/dithiol in the active site. Thioredoxin, with its redox-regulating and reactive oxygen species (ROS) scavenging activities, plays several important biologic roles both in intracellular and extracellular compartments. The purpose of this report was to quantify the relative expression of Trx in rat hippocampus following an oxidative stress-involving treatment such as kainic acid (KA) using real-time PCR and the 2-ΔΔCT method. The relative changes in expression of Trx mRNA in KA-treated and control animals were significantly different as 2.02 ± 0.77 and 1.0 ± 0.26, respectively ( P <0.05). Minimum and maximum n-fold changes in Trx expression in KA-treated and control animals were determined as (1.4-5.2) and (0.8-1.3), respectively. Thus, real-time PCR and the 2-ΔΔCT method for data analysis from real-time PCR were found to be an accurate and sensitive method for quantifying Trx mRNA levels.

Źródło:

Acta Biochimica Polonica; 2004, 51, 4; 1059-1065
0001-527X

Pojawia się w:

Acta Biochimica Polonica

Dostawca treści:

Biblioteka Nauki

/5

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Skocz do pozycji: 16.

Tytuł:

Quantification of thioredoxin mRNA expression in the rat hippocampus by real-time PCR following oxidative stress.

Autorzy:

Yalcin, Ayfer

Powiązania:

https://bibliotekanauki.pl/articles/1041529.pdf

Data publikacji:

2004

Wydawca:

Polskie Towarzystwo Biochemiczne

Tematy:

kainic acid
real-time polymerase chain reaction
oxidative stress
thioredoxin

Opis:

Thioredoxin (Trx) is a multifunctional protein with a redox-active disulfide/dithiol in the active site. Thioredoxin, with its redox-regulating and reactive oxygen species (ROS) scavenging activities, plays several important biologic roles both in intracellular and extracellular compartments. The purpose of this report was to quantify the relative expression of Trx in rat hippocampus following an oxidative stress-involving treatment such as kainic acid (KA) using real-time PCR and the 2-ΔΔCT method. The relative changes in expression of Trx mRNA in KA-treated and control animals were significantly different as 2.02 ± 0.77 and 1.0 ± 0.26, respectively ( P <0.05). Minimum and maximum n-fold changes in Trx expression in KA-treated and control animals were determined as (1.4-5.2) and (0.8-1.3), respectively. Thus, real-time PCR and the 2-ΔΔCT method for data analysis from real-time PCR were found to be an accurate and sensitive method for quantifying Trx mRNA levels.

Źródło:

Acta Biochimica Polonica; 2004, 51, 4; 1087-1090
0001-527X

Pojawia się w:

Acta Biochimica Polonica

Dostawca treści:

Biblioteka Nauki

/5

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Skocz do pozycji: 17.

Tytuł:

A universal method for the identification of genes encoding amatoxins and phallotoxins in poisonous mushrooms

Autorzy:

Woloszyn, A.
Kotlowski, R.

Powiązania:

https://bibliotekanauki.pl/articles/875623.pdf

Data publikacji:

2017

Wydawca:

Narodowy Instytut Zdrowia Publicznego. Państwowy Zakład Higieny

Tematy:

identification method
gene encoding
amatoxin
phallotoxin
poisonous mushroom
polymerase chain reaction

Opis:

Background. As the currently known diagnostic DNA targets amplified in the PCR assays for detection of poisonous mushrooms have their counterparts in edible species, there is a need to design PCR primers specific to the genes encoding amanitins and phallotoxins, which occur only in poisonous mushrooms. Objective. The aim of the study was testing of PCR-based method for detection of all genes encoding hepatotoxic cyclic peptides - amanitins and phallotoxins present in the most dangerous poisonous mushrooms. Material and Methods. Degenerate primers in the PCR were designed on the basis of amanitins (n=13) and phallotoxins (n=5) genes in 18 species of poisonous mushrooms deposited to Genbank of the National Center for Biotechnology Information. Results. The specificity of the PCR assays was confirmed against 9 species of edible mushrooms, death cap - Amanita phalloides and panther cap - Amanita pantherina. Conclusions. Designed two couples of PCR-primers specific to amanitins and phallotoxins genes can be recommended for detection of Amanita phalloides and other mushroom species producing hepatotoxic cyclic peptides - amanitins and phallotoxins.
Wprowadzenie. Ponieważ, obecnie znane diagnostyczne cele molekularne w genomach trujących grzybów kapeluszowych amplifikowane metodą PCR mają swoje odpowiedniki u grzybów jadalnych, istnieje potrzeba zastosowania specyficznych sekwencji starterowych wobec amanityn i fallotoksyn, występujących jedynie u grzybów trujących. Cel. Celem prowadzonych badań było sprawdzenie przydatności sekwencji starterowych do reakcji PCR specyficznych wobec wszystkich aktualnie poznanych genów kodujących hepatotoksyczne cykliczne peptydy - amanityny oraz fallotoksyny trujących grzybów kapeluszowych. Materiał i Metody. Sekwencje oligonokleotydowe starterów do reakcji PCR zaprojektowane zostały w oparciu o zdeponowane w Genbanku geny amanityn (n=13) oraz fallotoksyn (n=5). Wyniki. Specyficzność opracowanych testów PCR potwierdzono wobec 9 gatunków grzybów jadalnych oraz muchomora sromotnikowego - Amanita phalloides, jak i muchomora plamistego - Amanita pantherina. Wnioski. Zastosowane sekwencje starterowe do wykrywania genów kodujących amanityny i fallotoksyny metodą PCR, mogą być wykorzystane do wykrywania muchomora sromotnikowego oraz innych gatunków zdolnych do syntezy amanityn oraz fallotoksyn.

Źródło:

Roczniki Państwowego Zakładu Higieny; 2017, 68, 3
0035-7715

Pojawia się w:

Roczniki Państwowego Zakładu Higieny

Dostawca treści:

Biblioteka Nauki

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Skocz do pozycji: 18.

Tytuł:

Principles and applications of Ligation Mediated PCR methods for DNA-based typing of microbial organisms

Autorzy:

Krawczyk, Beata
Kur, Józef
Stojowska-Swędrzyńska, Karolina
Śpibida, Marta

Powiązania:

https://bibliotekanauki.pl/articles/1038839.pdf

Data publikacji:

2016

Wydawca:

Polskie Towarzystwo Biochemiczne

Tematy:

rapid methods
molecular epidemiology
genotyping
microbial phylogenetics
PCR (polymerase chain reaction)

Opis:

A significant number of DNA-based techniques has been introduced into the field of microorganisms' characterization and taxonomy. These genomic fingerprinting methods were developed to detect DNA sequence polymorphisms by using general principles, such as restriction endonuclease analysis, molecular hybridization, and PCR amplification. In recent years, some alternative techniques based on ligation of oligonucleotide adapters before DNA amplification by PCR, known as Ligation-Mediated PCR methods (LM PCR), have been successfully applied for the typing of microorganisms below the species level. These molecular methods include: Amplified Fragment Length Polymorphism (AFLP), Amplification of DNA fragments Surrounding Rare Restriction Sites (ADSRRS), PCR Melting Profiles (PCR MP), Ligation Mediated PCR/Shifter (LM PCR/Shifter), Infrequent-Restriction-Site Amplification (IRS PCR), double digestion Ligation Mediated Suppression PCR (ddLMS PCR). These techniques are now applied more and more often because they involve less time, are comparably inexpensive, and require only standard lab equipment. Here, we present a general review of this group of methods showing their possibilities and limitations. We also identify questions and propose solutions which may be helpful in choosing a particular LM PCR method for the achievement of the required goal.

Źródło:

Acta Biochimica Polonica; 2016, 63, 1; 39-52
0001-527X

Pojawia się w:

Acta Biochimica Polonica

Dostawca treści:

Biblioteka Nauki

/5

Artykuł

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Skocz do pozycji: 19.

Tytuł:

Isolation of the DNA fragment reflecting the open reading frame II of the I-18 C gene of Chironomus tentans by the polymerase chain reaction. IV. The product of the applied technologies

Autorzy:

Borowicz, B P

Powiązania:

https://bibliotekanauki.pl/articles/65915.pdf

Data publikacji:

1997

Wydawca:

Polska Akademia Nauk. Czytelnia Czasopism PAN

Tematy:

I-18 C gene
Chironomus tentans
polymerase chain reaction
DNA fragment

Opis:

The Polymerase Chain Reaction and other molecular technologies were applied to isolate the DNA fragment reflecting the Open Reading Frame II of the I-18 C gene. This research object was realized and the importance of this result has been discussed in view of the mechanisms of the regulation of gene expression.
Reakcja Łańcuchowa Polimerazy i inne technologie molekularne zostały zastosowane w celu izolacji fragmentu DNA odpowiadającego Otwartej Ramie Odczytu II genu I-18 C. Cel badawczy został zrealizowany i omówiono znaczenie tego wyniku w świetle mechanizmów regulacji ekspresji genu.

Źródło:

Journal of Plant Protection Research; 1997, 37, 1-2
1427-4345

Pojawia się w:

Journal of Plant Protection Research

Dostawca treści:

Biblioteka Nauki

/5

Artykuł

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Skocz do pozycji: 20.

Tytuł:

Segmented hybridization probes: modulating target affinity and base pairing selectivity

Autorzy:

Egetenmeyer, S.
Geiger, E.
Richert, C.

Powiązania:

https://bibliotekanauki.pl/articles/81071.pdf

Data publikacji:

2012

Wydawca:

Polska Akademia Nauk. Czytelnia Czasopism PAN

Tematy:

oligonucleotide
DNA
RNA
hybridization
base pairing
molecular biology
polymerase chain reaction

Źródło:

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2012, 93, 3
0860-7796

Pojawia się w:

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

Dostawca treści:

Biblioteka Nauki

/5

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Skocz do pozycji: 21.

Tytuł:

Polymorphism of DNA in nematodes of genus Trichinella Railliet, 1895 according to the data of polymerase chain reaction

Autorzy:

Shendrick, A.
Benedictov, I.
Bessonov, A.

Powiązania:

https://bibliotekanauki.pl/articles/838885.pdf

Data publikacji:

1998

Wydawca:

Polskie Towarzystwo Parazytologiczne

Tematy:

polymorphism
Trichinella pseudospiralis
Trichinella
nematode
polymerase chain reaction
DNA
Trichinella spiralis

Źródło:

Annals of Parasitology; 1998, 44, 3
0043-5163

Pojawia się w:

Annals of Parasitology

Dostawca treści:

Biblioteka Nauki

/5

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Skocz do pozycji: 22.

Tytuł:

Energia jądrowa i jej pokojowe wykorzystanie
Nuclear energy and its peaceful use

Autorzy:

Jóźwik, R.

Powiązania:

https://bibliotekanauki.pl/articles/236271.pdf

Data publikacji:

2017

Wydawca:

Wojskowy Instytut Techniczny Uzbrojenia

Tematy:

energia jądrowa
reakcja łańcuchowa
reaktor jądrowy
nuclear energy
chain reaction
nuclear reactor

Opis:

Artykuł przedstawia mechanizm powstawania energii jądrowej, typy reaktorów, które obecnie są, lub mogą być budowane i wykorzystywane dla celów pokojowych oraz historię energetyki jądrowej w Polsce.
The paper presents mechanism of nuclear energy production, types of reactors, which are and could be developed and used for peaceful purposes, and foundation of nuclear energy in Poland.

Źródło:

Problemy Techniki Uzbrojenia; 2017, 46, 142; 103-119
1230-3801

Pojawia się w:

Problemy Techniki Uzbrojenia

Dostawca treści:

Biblioteka Nauki

/5

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Skocz do pozycji: 23.

Tytuł:

Virulence and antibiotic resistance of Escherichia coli isolated from rooks

Autorzy:

Kmet, V.
Drugdova, Z.
Kmetova, M.
Stanko, M.

Powiązania:

https://bibliotekanauki.pl/articles/51141.pdf

Data publikacji:

2013

Wydawca:

Instytut Medycyny Wsi

Tematy:

virulence
antibiotic resistance
Escherichia coli
isolation
rook
polymerase chain reaction
DNA microarray

Opis:

With regard to antibiotic resistance studies in various model animals in the urban environment, the presented study focused on the rook, many behavioural and ecological aspects of which are important from an epidemiological point of view. A total of 130 Escherichia coli strains isolated from rook faeces during a two-year period (2011–2012) were investigated for antibiotic resistance and virulence. Resistance to ampicillin (60%) and streptomycin (40%) were the most frequent, followed by resistance to fluoroquinolones (ciprofloxacin-22% and enrofloxacin-24%), tetracycline (18%), cotrimoxazol (17%) and florfenicol (14%). Ceftiofur resistance occured in 10.7 % of strains and cefquinom resistance in 1.5 % of strains. Twenty-five E.coli strains with a higher level of MICs of cephalosporins (over 2mg/L of ceftazidime and ceftriaxon) and fluoroquinolones were selected for detection of betalactamase genes (CTX-M, CMY), plasmid-mediated quinolone resistance qnrS, integrase 1, and for APEC (avian pathogenic E.coli) virulence factors (iutA, cvaC, iss, tsh, ibeA, papC, kpsII). Genes of CTX-M1, CMY-2, integrase 1, papC, cvaC, iutA were detected in one strain of E.coli, and qnrS, integrase 1, iss, cvaC, tsh were detected in another E.coli. DNA microarray revealed the absence of verotoxin and enterotoxin genes and pathogenicity islands. The results show that rooks can serve as a reservoir of antibiotic-resistant E. coli with avian pathogenic virulence factors for the human population, and potentially transmit such E.coli over long distances.

Źródło:

Annals of Agricultural and Environmental Medicine; 2013, 20, 2
1232-1966

Pojawia się w:

Annals of Agricultural and Environmental Medicine

Dostawca treści:

Biblioteka Nauki

/5

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Skocz do pozycji: 24.

Tytuł:

Detection of nivalenol and deoxynivalenol chemotypes produced by Fusarium graminearum species complex isolated from barley in Iran using specific PCR assays

Autorzy:

Chehri, K.
Godini, R.

Powiązania:

https://bibliotekanauki.pl/articles/66014.pdf

Data publikacji:

2017

Wydawca:

Polska Akademia Nauk. Czytelnia Czasopism PAN

Tematy:

detection
nivalenol
deoxynivalenol
Fusarium graminearum
isolation
polymerase chain reaction
barley
trichothecene
Iran

Opis:

In order to identify trichothecenes chemotypes produced by Fusarium graminearum species complex (FGSC) isolated from barley, 68 barley samples were collected from markets in Kermanshah and Hamedan provinces, Iran. Thirty-one Fusarium isolates were obtained from grains and morphologically classified into three species FGSC (14), F. equiseti (9), and F. proliferatum (8). The identification of the members of FGSC was confirmed molecularly using Fg16F/Fg16R primers. Fusarium asiaticum isolates (4) were distinguished from other FGSC using Fg6CTPSf177/Fg16R primers. Polymerase chain reaction-based (PCRbased) detection of mycotoxin-synthesis-pathway gene was also used to determine the potential of the analysed strains to produce deoxynivalenol (DON), 15-acetyldeoxynivalenol (15-AcDON), 3-acetyldeoxynivalenol (3-AcDON), and nivalenol (NIV). Of 14 tested isolates, 10 and 4 isolates belonged to DON and NIV chemotype, respectively. Also, the results of DON chemotype survey using specific primers MinusTri7F/R and Tri315F/R showed 1 and 9 isolates produced 3-AcDON and 15-AcDON, respectively. These results show that DON was the most common chemotype in western Iran. To our knowledge, this is the first report on 15-AcDON, 3-AcDON, and NIV isolated from barley in Iran.

Źródło:

Journal of Plant Protection Research; 2017, 57, 3
1427-4345

Pojawia się w:

Journal of Plant Protection Research

Dostawca treści:

Biblioteka Nauki

/5

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Skocz do pozycji: 25.

Tytuł:

Acute SARS-CoV-2 infection and seropositivity among healthcare workers and medical students in summer 2020, Hungary

Autorzy:

Kosztin, Annamária
Merkely, Béla
Szabó, Attila J.
Blaha, Béla
Varga, Péter
Vásárhelyi, Barna
Vokó, Zoltán

Powiązania:

https://bibliotekanauki.pl/articles/2084806.pdf

Data publikacji:

2022-04-11

Wydawca:

Instytut Medycyny Pracy im. prof. dra Jerzego Nofera w Łodzi

Tematy:

polymerase chain reaction
medical students
seropositivity
COVID-19
SARS-CoV-2
healthcare workers

Opis:

ObjectivesThe aim was to compare the prevalence of acute infection and seropositivity of SARS-CoV-2 among healthcare workers (HCWs) and medical students.Material and MethodsA high-volume, single-center analysis was conducted in the period of July 1‒August 1, 2020, at the Semmelweis University. Naso- and oropharyngeal samples were collected for polymerase chain reaction (PCR), and blood samples for anti-SARS-CoV-2 IgG. A questionnaire was also administered about the infection symptoms and the obtained results were assessed by profession and site of care delivery.ResultsFrom the total cohort (N = 7948), 4478 (56%) and 3470 (44%) were health professionals and medical students, respectively. They were mainly female (67%), and the mean age of HCWs and students was 40 and 25 years, respectively. By profession, physicians (1.5%) and other HCWs (1.8%) showed a comparable SARS-CoV-2 exposure. International students had the highest (2.1%), whereas Hungarian students had the lowest (0.6%) prevalence of seropositivity. The highest prevalence was detected among the staff of COVID-19 wards (12.1%). By PCR, medical students showed the lowest occurrence of active infection with a prevalence of 0.17%, while physicians and other HCWs had a higher prevalence (1.46% and 1.71%, respectively). By site of care delivery, positive test results were the most frequent at COVID-19 wards (3.8%).ConclusionsPhysicians and other HCWs showed comparable SARS-CoV-2 seropositivity prevalence, approximately twice as high as in the general population of Budapest. Hungarian students had lower prevalence of seropositivity than this reference. High prevalence among international students suggests that they had imported the infection. The very high prevalence of documented exposure among staff members at COVID-19 wards urges for improving the safety measures.

Źródło:

International Journal of Occupational Medicine and Environmental Health; 2022, 35, 2; 209-216
1232-1087
1896-494X

Pojawia się w:

International Journal of Occupational Medicine and Environmental Health

Dostawca treści:

Biblioteka Nauki

/5

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Skocz do pozycji: 26.

Tytuł:

Diversity of Pseudomonas species associated with soft rot of plants in Poland

Autorzy:

Zolobowska, L
Pospieszny, H.

Powiązania:

https://bibliotekanauki.pl/articles/65211.pdf

Data publikacji:

2001

Wydawca:

Polska Akademia Nauk. Czytelnia Czasopism PAN

Tematy:

Pseudomonas fluorescens
Polska
Pseudomonas
polymerase chain reaction
occurrence
Pseudomonas putida
heterogeneity
identification
plant

Opis:

A total of 94 pectolytic and 60 nonpectolytic Pseudomonas isolates were obtained from 250 samples of rotted vegetable specimens representing various economically important vegetables. The isolates were identified on the basis of standard biochemical tests. Pseudomonas fluorescens biovar V and II and Pseudomonas putida were the most abundant species among pectolytic isolates and Pseudomonas fluorescens biovar I among nonpectolytic ones. Only 3 Pseudomonas viridiflava isolates were identified and all of them were obtained from potato. Isolates of pectolytic phenotype were scattered among nonpectolytic ones irrespective of their taxonomical status. Isolates identified biochemically, as Pseudomonas marginalis were also present in nonpectolytic group. PCR method is unsuitable for identification and differentiation of bacteria belonging to pectolytic fluorescens Pseudomonas group due to great diversity of species. However, the results of PCR amplification of the genes encoding pectate lyase suggest that genes responsible for production of this enzyme may also be present in isolates of nonpectolytic phenotype.
Z 250 prób, z różnych gatunków roślin warzywnych z objawami mokrej zgnilizny wyodrębniono 94 izolaty pektynolitycznych i 60 izolatów nie pektynolitycznych bakterii z rodzaju Pseudomonas. Identyfikację i różnicowanie izolatów przeprowadzono przy zastosowaniu standardowych testów fizjologiczno-biochemicznych. Spośród izolatów z grupy pektolitycznych Pseudomonas najliczniej występował gatunek P. fluorescens, który był reprezentowany przez 4 biowary (oprócz czwartego), przy czym dominowały biowary II i V. Mniej licznie występował gatunek P. putida, a P. viridiflava był reprezentowany jedynie przez 3 izolaty, pochodzące z ziemniaka. Podobna była struktura populacji izolatów z grupy niepektolitycznych Pseudomonas. Metoda PCR okazała się być mało przydatna do wykrywania i różnicowania izolatów z grupy pektolitycznych Pseudomonas ze względu na duże ich zróżnicowanie. Amplifikacja DNA metodą PCR wykazała, że geny kodujące enzym liazy pektynowej występują także w izolatach niepektolitycznych rodzaju Pseudomonas.

Źródło:

Journal of Plant Protection Research; 2001, 41, 1
1427-4345

Pojawia się w:

Journal of Plant Protection Research

Dostawca treści:

Biblioteka Nauki

/5

Artykuł

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Skocz do pozycji: 27.

Tytuł:

Isolation of the DNA fragment reflecting the open reading frame II of the I-18 C gene of Chironomus tentans by the polymerase chain reaction. II. The technology for the preparation of the template

Autorzy:

Borowicz, B P

Powiązania:

https://bibliotekanauki.pl/articles/65476.pdf

Data publikacji:

1997

Wydawca:

Polska Akademia Nauk. Czytelnia Czasopism PAN

Tematy:

gene expression
I-18 C gene
Chironomus tentans
polymerase chain reaction
DNA fragment

Opis:

The technology for the preparation of the template in the research aimed to isolate the DNA fragment reflecting the Open Reading Frame II of the I-18 C gene is presented i.e. the linearisation of the template by Bam H I digestion, the purification of the template and the estimation of the template concentration.
Przedstawiono technologię przygotowywania matrycy DNA w badaniach zmierzających do izolacji Otwartej Ramy Odczytu II genu I-18 C Chironomus tentans. Technologia ta obejmowała linearyzację DNA matrycy poprzez trawienie Bam H I oraz oczyszczenie matrycy i oznaczenie stężenia matrycowego DNA.

Źródło:

Journal of Plant Protection Research; 1997, 37, 1-2
1427-4345

Pojawia się w:

Journal of Plant Protection Research

Dostawca treści:

Biblioteka Nauki

/5

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Skocz do pozycji: 28.

Tytuł:

Cloning of the DNA fragments reflecting the open reading frame I and II of the I-18 C gene of Chironomus tentans. II. Preparation of the intermediate vector - bluescript plasmid for ligation with the inserts

Autorzy:

Borowicz, B P

Powiązania:

https://bibliotekanauki.pl/articles/66467.pdf

Data publikacji:

1999

Wydawca:

Polska Akademia Nauk. Czytelnia Czasopism PAN

Tematy:

cloning
I-18 C gene
plasmid
Chironomus tentans
polymerase chain reaction
DNA fragment

Opis:

The technology used for the cloning of the open reading frame (ORF) I and II reflecting DNA fragments of the I-18 C gene in regard to the preparation of the intermediate vector i.e. the bluescript for the ligation with the ORF I and II reflecting DNA fragments, is presented. The main steps include the Eco RV digestion of the bluescript plasmid, the phenol / methylene chloride extraction of the plasmid, dephosphorylation of the bluescript plasmid and finally its extraction with the phenol and ethanol precipitation.
Przedstawiono technologię, którą zastosowano do klonowania fragmentów DNA odzwierciedlających translacyjną otwartą ramę odczytu I i II genu I-18 C Chironomus tentans w zakresie: przygotowania wektora pośredniego - plazmidu blueskrypt do ligacji z wymienionymi sekwencjami wstawek. Główne etapy obejmują: trawienie plazmidu blueskrypt enzymem Eco RV, ekstrakcję plazmidu fenolem/ chlorkiem metylenu, defosforylację plazmidu blueskrypt, ostatecznie jego oczyszczenie poprzez ekstrakcję fenolową i precypitację etanolem.

Źródło:

Journal of Plant Protection Research; 1999, 39, 1
1427-4345

Pojawia się w:

Journal of Plant Protection Research

Dostawca treści:

Biblioteka Nauki

/5

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Skocz do pozycji: 29.

Tytuł:

Wild game as a reservoir of Anaplasma phagocytophilum in North-Western Poland

Autorzy:

Adamska, M
Skotarczak, B.

Powiązania:

https://bibliotekanauki.pl/articles/840225.pdf

Data publikacji:

2007

Wydawca:

Polskie Towarzystwo Parazytologiczne

Tematy:

parasite
Polska
reservoir
roe deer
bacteria
Anaplasma phagocytophilum
polymerase chain reaction
intracellular parasite
tick

Źródło:

Annals of Parasitology; 2007, 53, 2
0043-5163

Pojawia się w:

Annals of Parasitology

Dostawca treści:

Biblioteka Nauki

/5

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Skocz do pozycji: 30.

Tytuł:

Familial polyposis coli - inducing mutations in APC gene in Poland

Autorzy:

Pawlak, A L
Plawski, A
Smoczkiewicz, P
Kwiatkowska, J
Meissner, W
Krokowicz, P
West, S P

Powiązania:

https://bibliotekanauki.pl/articles/2046600.pdf

Data publikacji:

1997

Wydawca:

Polska Akademia Nauk. Czytelnia Czasopism PAN

Tematy:

familial adenomatous polyposis
protein truncation test
Polish population
mutation
polymerase chain reaction
heteroduplex analysis

Opis:

Screening for molecular changes within the adenomatous polyposis coli (APC) gene, exons 11-14 and the 5’ half of exon 15, encompassing the mutation cluster region within exon 15, was performed in 30 patients with Familial Polyposis Coli (FAP). All patients were studied by heteroduplex analysis (HA) and single strand conformation polymorphism (SSCP) and molecular changes were found in 7 cases. Protein truncation test (PTT) has been performed in 17 cases in which mutations have not been found earlier, and shortening of protein product was noted in 2 cases. In three cases common deletion of 5 bp at codon 1309 and in one 5 bp deletion at codon 1061 were found. In other cases the molecular changes were demonstrated as heteroduplexes in exon 14 (1 patient), in segments E and F (one patient each) of exon 15, and in two cases the heteroduplexes were within the overlapping sequences of segments E/F and F/G of exon 15, respectively. In families where the molecular changes were found by HA, 7 persons at high risk for FAP were found and advised to undergo regular endoscopic examinations. In three persons at risk the transfer of mutation was excluded.

Źródło:

Journal of Applied Genetics; 1997, 38, 1; 77-85
1234-1983

Pojawia się w:

Journal of Applied Genetics

Dostawca treści:

Biblioteka Nauki

/5

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Skocz do pozycji: 31.

Tytuł:

Construction of new GFP-tagged fusants for Trichoderma harzianum with enhanced biocontrol activity

Autorzy:

Kowsari, M.
Motallebi, M.
Zamani, R.M.

Powiązania:

https://bibliotekanauki.pl/articles/66878.pdf

Data publikacji:

2014

Wydawca:

Polska Akademia Nauk. Czytelnia Czasopism PAN

Tematy:

biomonitor
construction
Trichoderma harzianum
biological control
green fluorescent protein
real-time polymerase chain reaction

Opis:

Trichoderma is one of the most exploited biocontrol agents for the management of plant diseases. In biocontrol ecology, the critical factors are detection, and the monitoring and recovery of specific biocontrol agents either naturally present or deliberately released into the environment. Protoplast fusion is an appropriate tool for the improvement of biocontrol Trichoderma strains. Protoplast isolation from Trichoderma harzianum was achieved using 24 h culture age, 6.6 mg/ml Novazym L 1412 at 30°C which resulted the maximum protoplast yield of 5 × 108/ml. The self-fused protoplasts were regenerated and 12 fusants were selected based on their growth rate on 2% colloidal chitin medium. Next, a comparison was done for chitinase and antagonistic activity. Transcriptomic analysis based on quantitative real-time RT-PCR, demonstrated that T8-05 fusant expressed 1.5 fold of chit42 transcript as compared with the parental line. This fusant with 7.02±0.15U chitinase activity showed a higher growth inhibition rate (100%) than the parent strain, against Rhizoctonia solani. To obtain a genetically marked fusant that can be used as a biomonitor, the fusant was cotransformed with the gfp and amdS genes. The morphology and viability of selected cotransformant (FT8-7MK-05-2) was similar to the parent. Green fluorescing conidia were observed within the first 2 days of incubation in the soil, and this was followed by the formation of chlamydopores after 60 days. The colonisation of the gfp-tagged fusant was also monitored visually on R. solani sclerotia by scanning electron microscopy. Production of gfp-tagged fusant of Trichoderma spp. provides a potentially useful tool for monitoring hyphal growth patterns and the population of biocontrol agent isolates introduced into environmental systems.

Źródło:

Journal of Plant Protection Research; 2014, 54, 2
1427-4345

Pojawia się w:

Journal of Plant Protection Research

Dostawca treści:

Biblioteka Nauki

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Skocz do pozycji: 32.

Tytuł:

Detectability of tick-borne agents DNA in the blood of dogs, undergoing treatment for borreliosis

Autorzy:

Wodecka, B
Rymaszewska, A.
Sawczuk, M.
Skotarczak, B.

Powiązania:

https://bibliotekanauki.pl/articles/50622.pdf

Data publikacji:

2009

Wydawca:

Instytut Medycyny Wsi

Tematy:

dog
tick-borne agent
polymerase chain reaction
RFLP method
borreliosis
anaplasmosis
diagnostics
blood
treatment

Źródło:

Annals of Agricultural and Environmental Medicine; 2009, 16, 1; 9-14
1232-1966

Pojawia się w:

Annals of Agricultural and Environmental Medicine

Dostawca treści:

Biblioteka Nauki

/5

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Skocz do pozycji: 33.

Tytuł:

Occurrence of virulence genes among Campylobacter jejuni and Campylobacter coli isolates from domestic animals and children

Autorzy:

Andrzejewska, M.
Klawe, J.J.
Szczepanska, B.
Spica, D.

Powiązania:

https://bibliotekanauki.pl/articles/30802.pdf

Data publikacji:

2011

Wydawca:

Polska Akademia Nauk. Czytelnia Czasopism PAN

Tematy:

occurrence
virulence gene
Campylobacter jejuni
Campylobacter coli
isolate
domestic animal
child
polymerase chain reaction

Opis:

The presence of the flaA, cadF, cdtB and iam genes of Campylobacter spp. was determined with the PCR method. The materials to investigate were 56 C. jejuni and 23 C. coli strains isolated from clinical samples (children and domestic animals). It was found that all of the Campylobacter spp. isolates from children with diarrhoea and domestic animals had cadF gene, responsible for adherence. The flaA gene was present in all Campylobacter spp. isolates derived from children and cats. Occurrence of flaA gene was confirmed in 100% of C. jejuni strains obtained from dogs. The high prevalence of the cdtB gene associated with toxin production was observed in this study (100%-Campylobacter spp. isolates obtained from dogs and cats, 97.9%-Campylobacter spp. isolates from children). The isolates showed a wide variation for the presence of iam gene. The lowest prevalence (23.5%) was detected in Campylobacter spp. obtained from dogs. The highest rates of iam detection (91.6%) were revealed in C. coli isolates from children.

Źródło:

Polish Journal of Veterinary Sciences; 2011, 14, 2
1505-1773

Pojawia się w:

Polish Journal of Veterinary Sciences

Dostawca treści:

Biblioteka Nauki

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Skocz do pozycji: 34.

Tytuł:

Problems, limitations, and challenges in species identifcation of Ascomycota members on the basis of ITS regions

Autorzy:

Baturo-Cieśniewska, Anna
Pusz, Wojciech
Patejuk, Katarzyna

Powiązania:

https://bibliotekanauki.pl/articles/11543330.pdf

Data publikacji:

2020

Wydawca:

Polskie Towarzystwo Botaniczne

Tematy:

Ascomycota
fungi
internal transcribed spacer
molecular identification
polymerase chain reaction
barcode
rDNA
phylogenetic analysis

Opis:

The internal transcribed spacer (ITS) region is regarded as a formal fungal primary barcode with a high probability of the correct identification for a broad group of fungi. ITS sequences have been widely used to determine many fungal species and analysis of rDNA ITS is still one of the most popular tools used in mycology. However, this region is not equally variable in all groups of fungi; therefore, identification may be problematic and result in ambiguous data, especially in some species-rich genera of Ascomycota. For these reasons, identification based on rDNA ITS is usually complemented by morphological observations and analysis of additional genes. Reliable species identification of Ascomycota members is essential in diagnosing plant diseases, verifying air quality and the effectiveness of agronomic practices, or analyzing relationships between microorganisms. Therefore, the present study aimed to verify, using specific examples, the extent to which ITS sequence analysis is useful in species identification of pathogens and saprobionts from Ascomycota and demonstrate problems related to such identification in practice. We analyzed 105 ITS sequences of isolates originating from air and plant material. Basic local alignment search tool (BLASTn) significantly contributed to the reliable species identification of nearly 80% of isolates such as Arthrinium arundinis, Beauveria bassiana, Boeremia exigua, Cladosporium cladosporioides, Epicoccum nigrum, Nigrospora oryzae, Sclerotinia sclerotiorum, or Sordaria fimicola and members of the genera Alternaria and Trichoderma. However, for most isolates, additional morphological observations, information regarding the isolate origin and, where possible, a PCR with species-specific primers were helpful and complementary. Using our practical approach, we determined that ITS-based species identification and comparative analysis with GenBank sequences significantly helps identifying Ascomycota members. However, in many cases, this should be regarded as suggestive of a taxon because the data usually require the use of additional tools to verify the results of such analysis.

Źródło:

Acta Mycologica; 2020, 55, 1; 5512
0001-625X
2353-074X

Pojawia się w:

Acta Mycologica

Dostawca treści:

Biblioteka Nauki

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Skocz do pozycji: 35.

Tytuł:

Adaptor-mediated amplification of minute amounts of severely fragmented ancient nucleic acids

Autorzy:

Pusch, C M
Blin, N.
Broghammer, M.
Nicholson, G.J.
Scholz, M.

Powiązania:

https://bibliotekanauki.pl/articles/2042022.pdf

Data publikacji:

2001

Wydawca:

Polska Akademia Nauk. Czytelnia Czasopism PAN

Tematy:

mitochondrial DNA
genetics
nucleic acid
ancient DNA
polymerase chain reaction
DNA
cDNA
sex typing

Źródło:

Journal of Applied Genetics; 2000, 41, 4; 303-315
1234-1983

Pojawia się w:

Journal of Applied Genetics

Dostawca treści:

Biblioteka Nauki

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Skocz do pozycji: 36.

Tytuł:

The use amelogenin gene polymorphism in PCR embryo sexing in bovine IVF embryos

Autorzy:

Lechniak, D
Cumming, J R

Powiązania:

https://bibliotekanauki.pl/articles/2046605.pdf

Data publikacji:

1997

Wydawca:

Polska Akademia Nauk. Czytelnia Czasopism PAN

Tematy:

polymorphism
sex chromosome
Y chromosome
bovine embryo
sex
amelogenin gene
polymerase chain reaction
embryo

Opis:

The present study describes a rapid, simple method of bovine IVF embryo sexing by use of PCR technique. A pair of primers corresponding to the bovine amelogenin sequence has been used. The Rapid Cycler (Idaho Technology, USA) used in the current experiment enabled the PCR programme consisting of 55 cycles to be completed in less than 40 minutes. Therefore the total sexing procedure could be performed in less than 90 minutes. The described method succeded in case of 85% analysed embryos.

Źródło:

Journal of Applied Genetics; 1997, 38, 1; 45-49
1234-1983

Pojawia się w:

Journal of Applied Genetics

Dostawca treści:

Biblioteka Nauki

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Skocz do pozycji: 37.

Tytuł:

The use of nuclear energy for military and civilian purposes safety in the nuclear power industry

Autorzy:

Jóźwik, R.

Powiązania:

https://bibliotekanauki.pl/articles/347346.pdf

Data publikacji:

2017

Wydawca:

Akademia Wojsk Lądowych imienia generała Tadeusza Kościuszki

Tematy:

nuclear energy
chain reaction
nuclear reactor
safety systems
programme for the nuclear power industry

Opis:

The purpose of the article was to gather the basic information about the mechanism behind nuclear energy formation and the types of reactors, already built worldwide or potentially planned for construction in the near future, and to present the history of the beginnings of nuclear power in Poland. The issues of the safety of reactors, independent safety assurance systems and systems for emergency shutdown of a reactor are discussed in more detail. The problem of responsibility for the safety of nuclear equipment is also examined, including the relevant authority and method for such safety inspection. The initiatives taken in Poland in connection with the programme for the nuclear power industry are also described.

Źródło:

Zeszyty Naukowe / Wyższa Szkoła Oficerska Wojsk Lądowych im. gen. T. Kościuszki; 2017, 3; 106-123
1731-8157

Pojawia się w:

Zeszyty Naukowe / Wyższa Szkoła Oficerska Wojsk Lądowych im. gen. T. Kościuszki

Dostawca treści:

Biblioteka Nauki

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Skocz do pozycji: 38.

Tytuł:

Wild game as a reservoir of Anaplasma phagocytophilum in North-Western Poland

Autorzy:

Adamska, M.
Skotarczak, B.

Powiązania:

https://bibliotekanauki.pl/articles/2143862.pdf

Data publikacji:

2007

Wydawca:

Polskie Towarzystwo Parazytologiczne

Tematy:

parasite
Polska
reservoir
roe deer
bacteria
Anaplasma phagocytophilum
polymerase chain reaction
intracellular parasite
tick

Opis:

Background. The aim of the study was to determine the role of game animals as reservoirs of Anaplasma phagocytophilum, a bacteria species transmitted by Ixodes ricinus ticks, from north−western Poland (Zachodniopomorskie vovoidship). The area under question is endemic for A. phagocytophilum. Material and methods. Blood and spleen samples were taken from 72 roe deer between April and December 2003. Animals culled during winter did not harbour ticks, on the other hand 155 individuals of Ixodes ricinus were collected from 35 of 43 animals taken during spring. We tested all samples for A. phagocytophilum by PCR amplification of the msp2 gene. An individual was considered infected if pathogens were detected in at least one isolate (blood or a tissue sample). Results. DNA from A. phagocytophilum was not found in isolates from ticks collected from the animals. The general level of infection for the roe deer was 31.94% (23/72). DNA of A. phagocytophilum was most commonly detected in blood samples; only in three cases was anaplasma DNA detected in spleen and not in blood. Ruminants seem to be the most competent reservoir for A. phagocytophilum in north−western Poland.

Źródło:

Wiadomości Parazytologiczne; 2007, 53, 2; 103-107
0043-5163

Pojawia się w:

Wiadomości Parazytologiczne

Dostawca treści:

Biblioteka Nauki

/5

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Skocz do pozycji: 39.

Tytuł:

Diagnosis of Pneumocystis carinii infection by a serum polymerase chain reaction

Autorzy:

Golab, E.
Sobolewska, A.
Dzbenski, T.
Bitkowska, E.

Powiązania:

https://bibliotekanauki.pl/articles/836353.pdf

Data publikacji:

1998

Wydawca:

Polskie Towarzystwo Parazytologiczne

Tematy:

Pneumocystis carinii
infection
serum
diagnosis
laboratory diagnosis
pneumonia
polymerase chain reaction
rRNA gene
T cell

Źródło:

Annals of Parasitology; 1998, 44, 3
0043-5163

Pojawia się w:

Annals of Parasitology

Dostawca treści:

Biblioteka Nauki

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Skocz do pozycji: 40.

Tytuł:

Isolation of the DNA fragment reflecting the open reading frame II of the I-18 C gene of Chironomus tentans by the polymerase chain reaction. V. Different mechanisms of regulation regarding the open reading frames

Autorzy:

Borowicz, B P

Powiązania:

https://bibliotekanauki.pl/articles/64980.pdf

Data publikacji:

1997

Wydawca:

Polska Akademia Nauk. Czytelnia Czasopism PAN

Tematy:

regulation mechanism
gene expression
I-18 C gene
Chironomus tentans
polymerase chain reaction
DNA fragment

Opis:

Different mechanisms of regulation regarding the Open Reading Frames (ORFs) have been discussed to have a broader perspective that is necessary to evaluate the role of the ORFs of the I-18 C gene. This consideration includes the ORF II of the I-18 C gene which is the object of the presented research.
Omówiono różnorodne mechanizmy regulacji dotyczące Otwartych Ram Odczytu, w celu wytworzenia szerszego spojrzenia na to zagadnienie, które jest potrzebne do oceny roli poszczególnych Otwartych Ram Odczytu (ORF) genu I-18 C. Omówienie to dotyczy również ORF II genu I-18 C, co było przedmiotem zaprezentowanych badań.

Źródło:

Journal of Plant Protection Research; 1997, 37, 1-2
1427-4345

Pojawia się w:

Journal of Plant Protection Research

Dostawca treści:

Biblioteka Nauki

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Skocz do pozycji: 41.

Tytuł:

The use reverse transcription and polymerase chain reaction [RT-PCR] for the detection of hop latent viroid [HLVd]

Autorzy:

Cajza, M
Wypijewski, K.
Pospieszny, H.

Powiązania:

https://bibliotekanauki.pl/articles/65773.pdf

Data publikacji:

1997

Wydawca:

Polska Akademia Nauk. Czytelnia Czasopism PAN

Tematy:

hop latent viroid
reverse transcription
viroid
hop
plant pathogen
polymerase chain reaction
detection
DNA amplification

Opis:

The simple method of nucleic acids extraction, based on guanidine thiocyanate extraction buffer, was sufficient for obtaining a good templates for RT-PCR. The RT-PCR reactions were performer from the start to the end (both the reverse transcription and the HLVd-cDNA amplification) in the same reaction mixture and in the very small volume of reaction (10 μI). Both pairs of primers designed by authors were good for reverse transcription and later for amplification of the HLVd-cDNA. The presence of gelatin as a stabilizer of DNA polymerase was indispensable for successful performance of RT-PCR.
Wiroidy, najmniejsze obecnie znane patogeny roślin, zbudowane z pojedynczej nici kolistego RNA, nie zawierające w swej budowie i nie kodujące żadnych białek, są groźnymi patogenami roślin wyższych, rozpowszechnionymi we wszystkich rejonach świata, szczególnie w uprawach roślin rozmnażanych wegetatywnie. Wiroid latentny chmielu (ang. hop latent viroid - HLVd) został wykryty dopiero w 1988 roku w chmielu. Do tej pory odnotowano go w rejonach uprawy chmielu na całym świecie. Chmiel, jedyny znany żywiciel tego patogena, ulega tylko infekcjom utajonym (latentnym). HLVd powoduje zarówno spadek masy plonu szyszek jak i zawartości alfa-kwasów w szyszkach. Bezobjawowe infekcje oraz brak białek strukturalnych i innych produktów translacji powodują, że obecnie patogena tego można wykrywać tylko przy pomocy elektroforezy (metoda bardzo zawodna, wymagająca wysokiego stężenia RNA patogena w tkance), sond hybrydyzacyjnych i rozwijanej w ostatnich latach metody RT-PCR. Podczas opracowywania RT-PCR do wykrywania HLVd w ekstraktach kwasów nukleinowych wykorzystywano dwie pary primerów specyficznych dla sekwencji nukleotydowej HLVd, charakteryzujących się różnymi temperaturami topnienia produktu. Wiroida wykrywano w ekstraktach kwasów nukleinowych z blaszek liściowych i z ogonków liściowych chmielu. Uproszczona metoda guanidynowa do izolacji totalnych kwasów nukleinowych okazała się wystarczająca do przeprowadzenia reakcji RT-PCR. Opracowana metoda charakteryzowała się bardzo dużą czułością, specyficznością (produkty amplifikacji cDNA-HLVd uzyskiwano tylko z próbek materiału roślinnego pochodzącego z roślin zawierających tego wiroida) i szybkością wykonania (dwa dni łącznie z ekstrakcją kwasów nukleinowych). Ponadto w zastosowanej metodzie opracowano warunki do przeprowadzenia zarówno odwrotnej transkrypcji i następnie amplifikacji powstałego cDNA wiroida w jednej probówce, w tej samej mieszaninie reakcyjnej . Oprócz tego wszystkie reakcje RT-PCR prowadzono w bardzo małej objętości równej 10 μl (2 μl zawiesiny kwasów nukleinowych i 8 μl mieszaniny reakcji RT-PCR). Maksymalne uproszczenie metody, wielokrotne obniżenie kosztów reakcji oraz charakterystyczna dla RT-PCR czułość, specyficzność i szybkość przeprowadzenia testu sprawiają, że może być ona wykorzystywana do rutynowego wykrywania nie tylko HLVd ale również innych wiroidów, wirusoidów i wirusów RNA.

Źródło:

Journal of Plant Protection Research; 1997, 37, 1-2
1427-4345

Pojawia się w:

Journal of Plant Protection Research

Dostawca treści:

Biblioteka Nauki

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Skocz do pozycji: 42.

Tytuł:

Some evidence for skewed mating type distribution in Iranian populations of Rhynchosporium commune, the cause of barley scald disease

Autorzy:

Arzanlou, M.
Karimi, K.
Mirabi, F.

Powiązania:

https://bibliotekanauki.pl/articles/66749.pdf

Data publikacji:

2016

Wydawca:

Polska Akademia Nauk. Czytelnia Czasopism PAN

Tematy:

mating type
distribution
Rhynchosporium commune
barley
scald disease
foliar disease
scald
multiplex polymerase chain reaction

Opis:

Rhynchosporium commune (formerly known as Rhynchosporium secalis), the causal agent of scald disease on barley, is known to spread asexually by splash dispersed conidia. However, there are multiple lines of evidence for the possibility of a clandestine sexual cycle occurrence in this species including extensive genotypic diversity, equal distribution of mating type alleles across the world and expression of mating type genes. In the current study, the potential for the occurrence of a sexual cycle amongst the Iranian population of R. commune was assessed by analyzing distribution and frequency of the mating type alleles at both micro and macrospatial scales. A total of 95 single-conidial R. commune isolates were obtained from different barley fields in Kurdistan province. Previously designed primers were applied in a multiplex PCR assay to study distribution and frequency of the mating type alleles within and between populations. Totally, 67 isolates were determined as MAT1-1 and the remaining 28 isolates as MAT1-2 throughout the sampling counties. The results obtained at a macro-spatial scale revealed that unlike Kamyaran county (both MAT1-1 and MAT1-2 at an equal ratio), an unequal distribution of mating type genes was dominant among R. commune isolates in both Mariwan and Dehgolan counties. Our findings support a predominantly asexual reproduction for Mariwan and Dehgolan counties and the possibility of sexual stage occurrence in Kamyarna county.

Źródło:

Journal of Plant Protection Research; 2016, 56, 3
1427-4345

Pojawia się w:

Journal of Plant Protection Research

Dostawca treści:

Biblioteka Nauki

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Skocz do pozycji: 43.

Tytuł:

Isolation of the DNA fragment reflecting the open reading frame II of the I-18 C gene of Chironomus tentans by the polymerase chain reaction. III. The DNA amplification technology

Autorzy:

Borowicz, B P

Powiązania:

https://bibliotekanauki.pl/articles/66760.pdf

Data publikacji:

1997

Wydawca:

Polska Akademia Nauk. Czytelnia Czasopism PAN

Tematy:

gene expression
I-18 C gene
Chironomus tentans
polymerase chain reaction
DNA fragment
DNA amplification

Opis:

The technology used to amplify and isolate the DNA fragment reflecting the Open Reading Frame II of the I-18 C gene of Chironomus tentans is described i.e. the Polymerase Chain Reactioin and the agarose gel electrophoresis of this reaction product, as well as the blunting reaction of the product and its isolation.
Opisano technologię zastosowaną do powielenia i izolacji fragmentu DNA odpowiadającego Otwartej Ramie Odczytu II genu I-18 C Chironomus tentans, tj. Reakcję Łańcuchową Polimerazy (PCR) oraz elektroforezę w żelu agarozowym produktu tej reakcji jak również reakcję tzw. Stępiania końców wyżej wymienionego produktu i ostatecznie jego izolację .

Źródło:

Journal of Plant Protection Research; 1997, 37, 1-2
1427-4345

Pojawia się w:

Journal of Plant Protection Research

Dostawca treści:

Biblioteka Nauki

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Skocz do pozycji: 44.

Tytuł:

HybProbes-based real-time PCR assay for rapid detection of equine herpesvirus type 2 DNA

Autorzy:

Osinska, E.
Golke, A.
Slonska, A.
Cymerys, J.
Banbura, M.W.
Dzieciatkowski, T.

Powiązania:

https://bibliotekanauki.pl/articles/30252.pdf

Data publikacji:

2012

Wydawca:

Polska Akademia Nauk. Czytelnia Czasopism PAN

Tematy:

rapid detection
equine herpesvirus
real-time polymerase chain reaction
horse
herpesvirus
Gammaherpesvirinae
Rhadinovirus
veterinary virology

Opis:

Equid herpesvirus type 2 (EHV-2) together with equid herpesvirus type 5 are members of Gammaherpesvirinae subfamily, genus Rhadinovirus. EHV-2 is one of major agents causing diseases of horses common worldwide. A possible role of EHV-2 in reactivating latent equid herpesvirus type-1 has been suggested, because reactivation of latent EHV-1 was always accompanied by EHV-2 replication. Variety techniques, including cell culture, PCR and its modifications, have been used to diagnose EHV-2 infections. The aim of this study was to develop, optimize and determine specificity of real-time PCR (qPCR) for EHV-2 DNA detection using HybProbesR chemistry and to evaluate clinical samples with this method. The analytical sensitivity of assay was tested using serial dilutions of viral DNA in range between 70 and 7x105 copies/ml. The limit of detection (LOD) was calculated using probit analysis and was determined as 56 copies/ml. In further studies 20 different clinical samples were tested for the presence of EHV-2. Described in-house qPCR method detected viral DNA in 5 of 20 specimens used. The results of this work show that developed HybProbes-based real-time PCR assay is very reliable and valuable for detection and quantification of equid herpesvirus type 2 DNA in different clinical samples. The high level of sensitivity, accuracy and rapidity provided by the LightCycler 2.0 instrument are favorable for the use of this system in the detection of EHV-2 DNA in veterinary virology.

Źródło:

Polish Journal of Veterinary Sciences; 2012, 15, 3
1505-1773

Pojawia się w:

Polish Journal of Veterinary Sciences

Dostawca treści:

Biblioteka Nauki

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Skocz do pozycji: 45.

Tytuł:

Differentiation of Listeria monocytogenes on the basis of hemolytic and phosphatidylinositol specific phospholipase C activity and by PCR method

Autorzy:

Kotlowski, R.
Kaczmarek, M.
Kur, J.
Rudnicka, W.

Powiązania:

https://bibliotekanauki.pl/articles/1372565.pdf

Data publikacji:

1996

Wydawca:

Instytut Rozrodu Zwierząt i Badań Żywności Polskiej Akademii Nauk w Olsztynie

Tematy:

phosphatidylinositol-specific phospholipase C
phospholipase C
Listeria monocytogenes
bacteria
food preservation
polymerase chain reaction
hemolysis

Opis:

The possibility of differentiation of L.monocytogenes from other Listeria species on the basis of hemolytic activity, the production of phosphatidylinositol-specific phospholipase C (PI-PLC) and the polymerase chain reaction (PCR) for the amplification of a DNA fragment of listeriolysine O (hly A) gene was compared. The screening of Listeria colonies for PI-PLC activity allowed to distinguish the pathogenic for humans L.monocytogenes bacteria from the majority of non-pathogenic Listeria spp. The amplification of DNA from Listeria lysates with two primers selected in area of the hly A gene made possible the differentiation of L.monocytogenes from other Listeria species, including hemolytic L.ivanovii and L.seeligeri bacteria as well as hemolytic or PI-PLC positive L.innocua strains.
Żywność zanieczyszczona Listeria monocytogenes byía notowana jako źródło zakażeń pokarmowych o charakterze epidemicznym w krajach w Europie i Ameryce Północnej. W związku z tym koniecznością się stalo opracowanie szybkich i czuîych testów wykrywania i identyfikacji pałeczek L. monocytogenes. W pracy porównano przydatność trzech testów do odróżniania chorobotwórczych dla człowieka bakterii L. monocytogenes od innych niepatogennych gatunków Listeria. Uzyskane wyniki wskazują, na niewielką przydatność oceny aktywności hemolitycznej dla różnicowania szczepów L. monocytogenes od niechorobotwórczych dla ludzi szczepów L. ivanovii, L. seeligeri oraz L. innocua. Różnicowanie takich szczepów w oparciu o aktywność fosfolipazy C fosfatydyloinozytolu wykazuje wyższą specyficzność. Cechę taką wykazywały wszystkie badane szczepy L. monocytogenes i tylko jeden szczep L. innocua spośród 9 niepatogennych szczepów Listeria. Amplifikacja fragmentu DNA w obrębie genu dla listeriolizyny O (hly A) L. monocytogenes, pozwoliła odróżnić wszystkie (18) szczepy L. monocytogenes od niepatogennych dla człowieka bakterii L. ivanovii, L. innocua, L. seeligeri, L. welshimeri oraz L. grayi. Trawienie produktu amplifikacji DNA enzymem Taq I, pozwoliło na wyodrębnienie dwóch typów w obrębie grupy badanych szczepów L. monocytogenes.

Źródło:

Polish Journal of Food and Nutrition Sciences; 1996, 05, 3; 99-109
1230-0322
2083-6007

Pojawia się w:

Polish Journal of Food and Nutrition Sciences

Dostawca treści:

Biblioteka Nauki

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Skocz do pozycji: 46.

Tytuł:

Genetic similarity of chosen Syringa species determined by the ISSR-PCR technique

Autorzy:

Rzepka-Plevnes, D
Smolik, M.
Tanska, K.

Powiązania:

https://bibliotekanauki.pl/articles/41611.pdf

Data publikacji:

2006

Wydawca:

Polska Akademia Nauk. Instytut Dendrologii PAN

Tematy:

Przelewice Dendrological Garden
genetic variability
Syringa
lilac
genetic diversity
taxonomy
ISSR technique
polymerase chain reaction

Opis:

Inter-simple sequence repeat (ISSR) amplification was used to analyze polymorphism of microsatellite sequences in the lilacs genom and to evaluate genetic diversity among seven lilacs species (Syringa × prestoniae McKelvey., S. reflexa K.C. Schneid., S. villosa Vahl, S. × chinensis Willd., S. meyeri K.C.Schneid., S. vulgaris L.and S. reticulata (Blume) var. amurensis (Rupr.)). The plant material was originated from the collection of Dendrological Garden in Przelewice.A total of 30 primers, containing different simple sequence repeat motifs were tested for amplification.Out of the 30 primers only 13 gave interpretable banding patterns in all lilacs species.A total of 182 ISSR fragments were generated with 13 primers of which 109 (60%) were polymorphic and 57 (31.2%) species-specific. ISSR–PCR with genomic DNAs of the showed lilacs yielded DNA fragmets ranging form 2200 to 123 bp in size.Species-specific ISSR fragments were detected for each lilacs accessions.UPGMA cluster analysis was used to construct a dendrogram and to estimate the genetic distances between lilacs species.The ISSR-based phylogeny was generally consistent with Syringa taxonomy based on morphological and phenological evidence.

Źródło:

Dendrobiology; 2006, 56; 61-67
1641-1307

Pojawia się w:

Dendrobiology

Dostawca treści:

Biblioteka Nauki

/5

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Skocz do pozycji: 47.

Tytuł:

Temporal expression of three conserved putative microRNAs in response of Citrus × Limon to Xanthomonas citri subsp. citri and Xanthomonas fuscans subsp. Aurantifolii

Autorzy:

Alizadeh, M.
Askari, H.
Najafabadi, M.S.

Powiązania:

https://bibliotekanauki.pl/articles/81264.pdf

Data publikacji:

2017

Wydawca:

Polska Akademia Nauk. Czytelnia Czasopism PAN

Tematy:

lemon
fruit canker
bacterial disease
Xanthomonas
microRNA
plant-pathogen interaction
reserve transcript polymerase chain reaction

Źródło:

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2017, 98, 3
0860-7796

Pojawia się w:

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

Dostawca treści:

Biblioteka Nauki

/5

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Skocz do pozycji: 48.

Tytuł:

Large-scale analysis of sequence tags in Xp11.4-11.3 and evaluation of candidate genes for X-linked ocular diseases

Autorzy:

Pusch, C M

Powiązania:

https://bibliotekanauki.pl/articles/2041943.pdf

Data publikacji:

2001

Wydawca:

Polska Akademia Nauk. Czytelnia Czasopism PAN

Tematy:

X chromosome
man
pseudogene
polymerase chain reaction
X-linked ocular disease
night vision
genetic disease

Źródło:

Journal of Applied Genetics; 2001, 42, 1; 89-102
1234-1983

Pojawia się w:

Journal of Applied Genetics

Dostawca treści:

Biblioteka Nauki

/5

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Skocz do pozycji: 49.

Tytuł:

Gene expression and peptide localization for LH-hCG receptor in porcine small and large luteal cells: possible regulation by opioid peptides

Autorzy:

Kaminski, T.
Gawronska, B.
Derecka, K.
Okrasa, S.
Przala, J.

Powiązania:

https://bibliotekanauki.pl/articles/69878.pdf

Data publikacji:

2000

Wydawca:

Polskie Towarzystwo Fizjologiczne

Tematy:

reverse transcription
opioid peptide
gene expression
immunocytochemistry
polymerase chain reaction
peptide localization
porcine luteal cell

Źródło:

Journal of Physiology and Pharmacology; 2000, 51, 2
0867-5910

Pojawia się w:

Journal of Physiology and Pharmacology

Dostawca treści:

Biblioteka Nauki

/5

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Skocz do pozycji: 50.

Tytuł:

The quantitative PCR technique resolves ambiguities concerning a small rearrangement of human chromosome 6q12-13

Autorzy:

Nowacka, J
Helszer, Z.
Walter, Z.
Plucienniczak, A.
Kaluzewski, B.

Powiązania:

https://bibliotekanauki.pl/articles/2048327.pdf

Data publikacji:

2001

Wydawca:

Polska Akademia Nauk. Czytelnia Czasopism PAN

Tematy:

duplication
chromosome
human chromosome
man
rearrangement
cytogenetic diagnosis
karyotype analysis
chromosome aberration
polymerase chain reaction
inversion

Źródło:

Journal of Applied Genetics; 2001, 42, 4; 541-545
1234-1983

Pojawia się w:

Journal of Applied Genetics

Dostawca treści:

Biblioteka Nauki

/5

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Skocz do pozycji: 51.

Tytuł:

Association of arylamine N-acetyltransferase [NAT1 and NAT2] genotypes with urinary bladder cancer risk

Autorzy:

Jaskula-Sztul, R
Sokolowski, W.
Gajecka, M.
Szyfter, K.

Powiązania:

https://bibliotekanauki.pl/articles/2041980.pdf

Data publikacji:

2001

Wydawca:

Polska Akademia Nauk. Czytelnia Czasopism PAN

Tematy:

tobacco smoking
human genetics
genotype
urinary bladder
polymerase chain reaction
cancer
acetylator genotype
arylamine N-acetyltransferase

Źródło:

Journal of Applied Genetics; 2001, 42, 2; 223-231
1234-1983

Pojawia się w:

Journal of Applied Genetics

Dostawca treści:

Biblioteka Nauki

/5

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Skocz do pozycji: 52.

Tytuł:

Identification and characterization of Pseudomonas syringae pv. syringae strains from various plants and geographical regions

Autorzy:

Khezri, M.
Mohammadi, M.

Powiązania:

https://bibliotekanauki.pl/articles/65669.pdf

Data publikacji:

2018

Wydawca:

Polska Akademia Nauk. Czytelnia Czasopism PAN

Tematy:

identification
Pseudomonas syringae pv.syringae
plant
phenotype
polymerase chain reaction
protein profile
plasmid
syringomycin
geographic region

Opis:

Pseudomonas syringae pv. syringae (Pss) constitutes a diverse group of bacterial strains that cause canker of stone fruits, blight of cereals and red streak of sugarcane. The purpose of this study was to determine how diverse Iranian strains of Pss are when they come from different hosts. We compared a total of 32 Pss strains isolated from stone fruits, barley, wheat and sugarcane from different geographical regions of Iran based on their phenotypic and molecular properties. Strains showed some variation regarding carbon and nitrogen utilization. Pss strains were similar in their protein banding patterns. Additional bands were found in sugarcane strains. Most strains showed one indigenous plasmid DNA and a few had two and some none. The genes of syrB and syrD encoding syringomycin synthesis and secretion, respectively, were amplified using specific primers in polymerase chain reaction. Syringomycin, producing strains amplified two DNA fragments of 752 and 446 bp representing syrB and syrD genes, respectively. Primer specificity was shown for Pss using various genera. Based on the results of this study, it is suggested that Pss strains from different hosts and geographical regions show diversity in phenotypic and molecular characters. It is thought that phenotypic variation is due to adaptation to specific hosts and niches for survival and pathogenicity.

Źródło:

Journal of Plant Protection Research; 2018, 58, 4
1427-4345

Pojawia się w:

Journal of Plant Protection Research

Dostawca treści:

Biblioteka Nauki

/5

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Skocz do pozycji: 53.

Tytuł:

Isolation of the DNA fragment reflecting the open reading frame II of the I-18 C gene of Chironomus tentans by the polymerase chain reaction. I. The technology for the preparation of the primers

Autorzy:

Borowicz, B P

Powiązania:

https://bibliotekanauki.pl/articles/65836.pdf

Data publikacji:

1997

Wydawca:

Polska Akademia Nauk. Czytelnia Czasopism PAN

Tematy:

phosphorylation
plant protection
I-18 C gene
purification
Chironomus tentans
polymerase chain reaction
DNA fragment
oligonucleotide

Opis:

The above mentioned technology in a case of the DNA fragment reflecting the Open Reading Frame II of the I-18 C gene is described i.e. the primers design, purification and phosphorylation of the oligonucleotide primers.
Dla określonego w tytule pracy, celu badań przedstawiono technologię przygotowywania starterów, w przypadku DNA odpowiadającego Otwartej Ramie Odczytu II genu I-18 C Chironomus tentans. Technologia ta obejmowała zaprojektowanie starterów dla PCR oraz oczyszczenie i fosforylację zsyntetyzowanych oligonukleotydowych starterów.

Źródło:

Journal of Plant Protection Research; 1997, 37, 1-2
1427-4345

Pojawia się w:

Journal of Plant Protection Research

Dostawca treści:

Biblioteka Nauki

/5

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Skocz do pozycji: 54.

Tytuł:

Identification of Clavibacter michiganensis subsp. michiganensis by the polymerase chain reaction [PCR]

Autorzy:

Cajza, M
Rezler, A.
Pospieszny, H.

Powiązania:

https://bibliotekanauki.pl/articles/65945.pdf

Data publikacji:

1997

Wydawca:

Polska Akademia Nauk. Czytelnia Czasopism PAN

Tematy:

tomato seed
pathogen
Clavibacter michiganensis
plant protection
bacteria
polymerase chain reaction
detection
DNA amplification
seed
identification

Opis:

The PCR offers the possibility of specific and very sensitive detection and/or identification of Cl. m. subsp. michiganensis in seeds. The described PCR method for identification of this pathogen is very fast (one day) and economical, because of the very small volume (10 μI) of the PCR reaction mixture. The method based on amplification of the bacterial plasmid DNA fragment may be very useful in routine identification of Cl. m. subsp. michiganensis from all other bacteria isolated from tomato seeds and may be an alternative tool in diagnostics, especially for the quarantine services.
Clavibacter michiganensis subsp. michiganensis jest bardzo groźnym patogenem kwarantannowym pomidora, wywołującym chorobę zwaną bakteryjnym rakiem pomidora. Bakteria ta łatwo przenosi się z zakażonymi nasionami i sadzonkami. Pomimo opracowania różnych technik diagnostycznych do jej wykrywania, nadal stanowi duży problem w diagnozowaniu chorób pomidora. Powszechnie stosowane w Polsce wykrywanie tej bakterii, bazujące na testach enzymoimmunologicznych (ELISA), immunofluorescencyjnych, hodowli na pożywkach półselektywnych i testach biologicznych, jest często zawodne ze względu na duże serologiczne zróżnicowanie poszczególnych szczepów tej bakterii, obecność wielu bakterii saprofitycznych, tworzących kolonie nie różniące się morfologicznie od kolonii właściwych dla Cl. m. subsp. michiganensis, czy też mało przydatne ze względu na długi czas trwania testu i obecność infekcji latentnych. Jedną z nowoczesnych metod wykrywania i identyfikacji bakterii jest amplifikacja fragmentów DNA charakterystycznych dla jej genomu przy pomocy reakcji PCR. Metoda ta dopiero zaczyna być powszechnie stosowana w diagnostyce bakterii (nieliczne doniesienia, w większości z ostatnich trzech lat). Opracowana metoda identyfikacji Cl. m. subsp. michiganensis spośród różnych bakterii saprofitycznych obecnych na nasionach pomidora, charakteryzuje się bardzo dużą czułością (do wykrycia Cl. m. subsp. michiganensis wystarczy 200-300 bakterii / 1 ml), specyficznością (produkty amplifikacji DNA bakteryjnego o oczekiwanej długości 614 bp, uzyskiwano tylko w próbkach, w których znajdował się DNA z Cl. m. subsp. michiganensis) oraz szybkością (jeden dzień, wraz z izolacją DNA bakteryjnego). Ponadto opracowana metoda charakteryzuje się bardzo niskimi kosztami, gdyż cala reakcja amplifikacji DNA zachodzi w objętości 10 μI (4 μI zawiesiny DNA i 6 μI mieszaniny reakcyjnej), co sprawia, że stosowanie tej metody w powszechnej diagnostyce chorób bakteryjnych będzie coraz częstsze i będzie ona coraz bardziej konkurencyjna w stosunku do innych, obecnie stosowanych.

Źródło:

Journal of Plant Protection Research; 1997, 37, 1-2
1427-4345

Pojawia się w:

Journal of Plant Protection Research

Dostawca treści:

Biblioteka Nauki

/5

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Skocz do pozycji: 55.

Tytuł:

Molecular characterization of the first internal transcribed spacer of rDNA of Parabronema skrjabini for the first time in sheep

Autorzy:

Hasheminasab, S.S.

Powiązania:

https://bibliotekanauki.pl/articles/6198.pdf

Data publikacji:

2015

Wydawca:

Polskie Towarzystwo Parazytologiczne

Tematy:

molecular characteristics
nematode
rDNA
Parabronema skrjabini
food-borne parasite
parasitic disease
sheep
polymerase chain reaction
Iran

Opis:

Parabronema skrjabini is a spirurid nematode of the family Habronematidae that lives in the abomasum of ruminants such as sheep and goats. The purpose of this study was to investigate the molecular aspects of Parabronema skrjabini in sheep. The worms were collected from sheep in Sanandaj (west of Iran). The first internal transcribed spacer (ITS) of ribosomal DNA (rDNA) nucleotide fragments of Parabronema skrjabini were amplified by polymerase chain reaction (PCR) using two pairs of specific primers (Para-Ir-R and Para-Ir-F). ITS1 homology in the sequence of this study was 69% compared with the sequence data in GenBank. To our knowledge, this is the first study in the world exploring the genetic diversity of P. skrjabini in sheep based on ITS1.

Źródło:

Annals of Parasitology; 2015, 61, 4
0043-5163

Pojawia się w:

Annals of Parasitology

Dostawca treści:

Biblioteka Nauki

/5

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Skocz do pozycji: 56.

Tytuł:

Detection and identification of banned processed animal protein in feedingstuffs by microscopic and PCR methods

Autorzy:

Weiner, A.
Golebiowska, A.
Paprocka, I.
Kwiatek, K.

Powiązania:

https://bibliotekanauki.pl/articles/30136.pdf

Data publikacji:

2012

Wydawca:

Polska Akademia Nauk. Czytelnia Czasopism PAN

Tematy:

detection
identification
processed animal protein
microscopic method
polymerase chain reaction
feeding stuff
meat meal
bone meal

Opis:

The aim of the study was to present the results of comparative evaluation of the usefulness of PCR and microscopic methods for the detection of Processed Animal Protein (PAP) in feedingstuffs. In the validation study, the limit of the detection for PCR was determined on 0.05% for beef, 0.1% for pork and 0.2% for poultry meat and bone meal (MBM). Among 62 doubtful samples of feedingstuffs examined by microscopic method 41 (66.13%) were found as positive. Based on the results obtained with the use of the microscopic and PCR methods it is possible to state that the molecular biology methods can, at present, be used as a supplementary method in PAP detection.

Źródło:

Polish Journal of Veterinary Sciences; 2012, 15, 1
1505-1773

Pojawia się w:

Polish Journal of Veterinary Sciences

Dostawca treści:

Biblioteka Nauki

/5

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Skocz do pozycji: 57.

Tytuł:

Analysis of the epidemiological factors influencing vulpine trichinellosis in ecologically different regions of Slovakia

Autorzy:

Hurnikova, Z
Bartkova, D.
Dubinsky, P.

Powiązania:

https://bibliotekanauki.pl/articles/839915.pdf

Data publikacji:

2006

Wydawca:

Polskie Towarzystwo Parazytologiczne

Tematy:

red fox
parasite
Trichinella
epidemiological factor
trichinellosis
Vulpes vulpes
parasitology
polymerase chain reaction
animal disease
Slovakia

Opis:

Introduction. In the Slovak Republic, trichinellosis circulates almost exclusively in the sylvatic cycle, with main reservoir host red fox and wild boar and sporadic occurrence of human outbreaks. A detailed study was performed in five ecologically different regions of eastern Slovakia with more profound regard to eco-geographical and anthropogenic influences to natural fox habitat. Material and methods. In total of 689 red foxes (Vulpes vulpes) hunted in selected regions in 2005/2006 was examined using artificial digestion method. Larvae obtained from infected samples were on the species level characterised using multiplex PCR analysis. Results. The study revealed a total prevalence of 15.6%, with most frequent occurrence of infected foxes in the mountain of the Volovské Vrchy (25.2%) where both human habitation and fox population are very dense. High prevalence rates were found in the Košická Kotlina Basin (19.6%) with urbanised landscape, concentrated human activities and low fox population and in national park of the High Tatras (15.8%) where the inhabitants and fox population are relatively low. In the remote localities of the Nízke Beskydy Highlands that represent ideal fox habitat free of any human impact, 14.2% of foxes harboured Trichinella larvae. The lowest occurrence of infected foxes (6.9%) was found in agrarian areas of the Východoslovenská Nížina Lowland, with relatively low inhabitants and fox population density. In all localities Trichinella britovi was the most important etiological agent of sylvatic trichinellosis.

Źródło:

Annals of Parasitology; 2006, 52, 3
0043-5163

Pojawia się w:

Annals of Parasitology

Dostawca treści:

Biblioteka Nauki

/5

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Skocz do pozycji: 58.

Tytuł:

Role of polymorphisms TNF.- alpha gene in the development of recurrent miscarriage after in vitro fertilization in the residentS of Odessa region of Ukraine

Autorzy:

Golovatyuk, K. P.

Powiązania:

https://bibliotekanauki.pl/articles/765441.pdf

Data publikacji:

2017

Wydawca:

Uniwersytet Mikołaja Kopernika w Toruniu. Wydział Nauk o Ziemi i Gospodarki Przestrzennej. Katedra Kultury Fizycznej

Tematy:

recurrent miscarriage, in vitro fertilization, tumor necrosis factor-α r, inheritance, polymorphism, genotype, polymerase chain reaction

Opis:

Golovatyuk K. P. Role of polymorphisms TNF.- alpha gene in the development of recurrent miscarriage after in vitro fertilization in the residentS of Odessa region of Ukraine. Journal of Education, Health and Sport. 2017;7(1):323 - 332. eISSN 2391-8306. DOI http://dx.doi.org/10.5281/zenodo.265805http://ojs.ukw.edu.pl/index.php/johs/article/view/4218    The journal has had 7 points in Ministry of Science and Higher Education parametric evaluation. Part B item 754 (09.12.2016).754 Journal of Education, Health and Sport eISSN 2391-8306 7© The Author (s) 2017;This article is published with open access at Licensee Open Journal Systems of Kazimierz Wielki University in Bydgoszcz, PolandOpen Access. This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and source are credited. This is an open access article licensed under the terms of the Creative Commons Attribution Non Commercial License(http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted, non commercial use, distribution and reproduction in any medium, provided the work is properly cited.This is an open access article licensed under the terms of the Creative Commons Attribution Non Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted, non commercial use, distribution and reproduction in any medium, provided the work is properly cited.The authors declare that there is no conflict of interests regarding the publication of this paper.Received: 02.01.2017. Revised 16.01.2017. Accepted: 24.01.2017. UDK 618.177-089.888.11:618.39-0796:575.162 ROLE OF POLYMORPHISMS TNF- alpha GENE IN THE DEVELOPMENT OF recurrent miscarriage after in vitro fertilization in THE  residentS of Odessa region of Ukraine K. P. Golovatyuk Odessa National medical University, Ukraine; nosenko.olena@gmail.com;  info@gameta.od.ua SummaryThe frequency of genotypes and allelic variants of the TNF-α gene (-238 G> A, -308 G> A, -1031 T > C), depending on the reproductive status and evaluation of its association with recurrent miscarriage (RM) in cycles in vitro fertilization (IVF) has been studied. The residents of the Odessaregion of Ukrainehave been under examination. It has been revealed that typing of SNPs of genes of the immune response TNF-α (rs1799964) and TNF-α (rs1800629) may be used as an early diagnostic method and pregravida prediction of reproductive losses in infertile women with RM after IVF. Genotypes AA -308G> A, (GA + AA) -308G> A and TC-1031C, (TC + CC) -1031C TNF-α gene were statistically significant associated with an increased risk of RM after IVF.Key words: recurrent miscarriage, in vitro fertilization, tumor necrosis factor-α r, inheritance, polymorphism, genotype, polymerase chain reaction.

Źródło:

Journal of Education, Health and Sport; 2017, 7, 1
2391-8306

Pojawia się w:

Journal of Education, Health and Sport

Dostawca treści:

Biblioteka Nauki

/5

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Skocz do pozycji: 59.

Tytuł:

Analysis of the epidemiological factors influencing vulpine trichinellosis in ecologically different regions of Slovakia

Autorzy:

Hurnikova, Z.
Bartkova, D.
Dubinsky, P.

Powiązania:

https://bibliotekanauki.pl/articles/2144345.pdf

Data publikacji:

2006

Wydawca:

Polskie Towarzystwo Parazytologiczne

Tematy:

red fox
parasite
Trichinella
epidemiological factor
trichinellosis
Vulpes vulpes
parasitology
polymerase chain reaction
animal disease
Slovakia

Opis:

Introduction. In the Slovak Republic, trichinellosis circulates almost exclusively in the sylvatic cycle, with main reservoir host red fox and wild boar and sporadic occurrence of human outbreaks. A detailed study was performed in five ecologically different regions of eastern Slovakia with more profound regard to eco-geographical and anthropogenic influences to natural fox habitat. Material and methods. In total of 689 red foxes (Vulpes vulpes) hunted in selected regions in 2005/2006 was examined using artificial digestion method. Larvae obtained from infected samples were on the species level characterised using multiplex PCR analysis. Results. The study revealed a total prevalence of 15.6%, with most frequent occurrence of infected foxes in the mountain of the Volovské Vrchy (25.2%) where both human habitation and fox population are very dense. High prevalence rates were found in the Košická Kotlina Basin (19.6%) with urbanised landscape, concentrated human activities and low fox population and in national park of the High Tatras (15.8%) where the inhabitants and fox population are relatively low. In the remote localities of the Nízke Beskydy Highlands that represent ideal fox habitat free of any human impact, 14.2% of foxes harboured Trichinella larvae. The lowest occurrence of infected foxes (6.9%) was found in agrarian areas of the Východoslovenská Nížina Lowland, with relatively low inhabitants and fox population density. In all localities Trichinella britovi was the most important etiological agent of sylvatic trichinellosis.

Źródło:

Wiadomości Parazytologiczne; 2006, 52, 3; 213-218
0043-5163

Pojawia się w:

Wiadomości Parazytologiczne

Dostawca treści:

Biblioteka Nauki

/5

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Skocz do pozycji: 60.

Tytuł:

Unidirectional orientation of twelve expressed tagged sites within 4o kb of human chromosomal region 22q13.1

Autorzy:

Pusch, C
Wang, Z.
Roe, B.
Blin, N.

Powiązania:

https://bibliotekanauki.pl/articles/2048293.pdf

Data publikacji:

1998

Wydawca:

Polska Akademia Nauk. Czytelnia Czasopism PAN

Tematy:

RNA
human chromosome
chromosome
hybridization
genetic change
linear map
polymerase chain reaction
DNA
cosmid
cDNA
transcriptional orientation

Opis:

The single copy sequence D22S16 from human chromosomal region 22q13.1 that carries a putative conserved gene, was used to probe a chromosome 22-specific cosmid library. Genomic sequencing of one positive, 40 kb long cosmid (C1155) revealed a hereto unmapped gene (a subunit of DNA-dependent RNA polymerase II, POLR2F), a SOX9-related sequence and 12 expressed sequence tags. Although not parts of one consecutive gene, all 12 ESTs and, in addition, the polymerase gene are oriented in the same transcriptional direction within the genomic sequence represented by cosmid C1155.

Źródło:

Journal of Applied Genetics; 1998, 39, 2; 199-204
1234-1983

Pojawia się w:

Journal of Applied Genetics

Dostawca treści:

Biblioteka Nauki

/5

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Skocz do pozycji: 61.

Tytuł:

Genotyping of bovine beta-lactoglobulin [LGB] by PCR-SSCP technique

Autorzy:

Kaminski, S
Zabolewicz, T

Powiązania:

https://bibliotekanauki.pl/articles/2046615.pdf

Data publikacji:

1997

Wydawca:

Polska Akademia Nauk. Czytelnia Czasopism PAN

Tematy:

AB genotype
SSCP method
cattle
genotype
AA genotype
BB genotype
beta-lactoglobulin
polymerase chain reaction
allele
identification

Opis:

A new method facilitating the identification of the two most common alleles (A and B) of the bovine beta-lacoglobulin (LGB) gene is described. The method is based on two steps: PCR amplification of 240 bp fragment of LGB gene followed by the single stranded conformation polymorphism (SSCP) detection. AA, AB and BB genotypes of LGB were identified with this technique. The PCR-SSCP is simple, accurate and relatively inexpensive. Additionally, this method has a potential to detect new variants within the amplified gene fragment.

Źródło:

Journal of Applied Genetics; 1997, 38, 4; 471-476
1234-1983

Pojawia się w:

Journal of Applied Genetics

Dostawca treści:

Biblioteka Nauki

/5

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Skocz do pozycji: 62.

Tytuł:

A simple method for extracting DNA from rhododendron plants infected with Phytophthora spp. for use in PCR

Autorzy:

Trzewik, A.
Nowak, K.J.
Orlikowska, T.

Powiązania:

https://bibliotekanauki.pl/articles/66480.pdf

Data publikacji:

2016

Wydawca:

Polska Akademia Nauk. Czytelnia Czasopism PAN

Tematy:

simple method
DNA extraction
rhododendron
leaf
plant infection
Phytophthora
polymerase chain reaction
detection
real-time PCR method

Opis:

Among the numerous protocols that describe the extraction of DNA, those relating to the isolation of DNA from infected plants, are rare. This study describes a rapid and reliable method of extracting a high quality and quantity of DNA from rhododendron leaves artificially infected with Phytophthora cactorum, P. cambivora, P. cinnamomi, P. citrophthora, and P. plurivora. The use of the modified Doyle and Doyle protocol (1987) allowed us to obtain high quantity and quality DNA (18.26 μg from 100 mg of the fresh weight of infected leaves at the ratios of A260/280 and A260/230 – 1.83 and 1.72, respectively), suitable for conventional polymerase chain reaction (PCR) and real-time PCR amplifications.

Źródło:

Journal of Plant Protection Research; 2016, 56, 1
1427-4345

Pojawia się w:

Journal of Plant Protection Research

Dostawca treści:

Biblioteka Nauki

/5

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Skocz do pozycji: 63.

Tytuł:

A small-scale survey of hantavirus in mammals from eastern Poland

Autorzy:

Wojcik-Fatla, A.
Zajac, V.
Knap, J.P.
Sroka, J.
Cisak, E.
Sawczyn, A.
Dutkiewicz, J.

Powiązania:

https://bibliotekanauki.pl/articles/51577.pdf

Data publikacji:

2013

Wydawca:

Instytut Medycyny Wsi

Tematy:

hantavirus
Bunyaviridae
mammal
small mammal
Microtus agrestis
Myodes glareolus
Sorex araneus
epidemiology
polymerase chain reaction
flood
Polska

Opis:

Samples of 30 dead small mammals each were collected on area ‘A’ located in eastern Poland which is exposed to flooding by the Vistula river, and on the area ‘B’, also located in eastern Poland but not exposed to flooding. Kidneys and livers of the mammals were examined by the PCR and nested PCR methods for the presence of hantavirus RNA. Out of 7 species of small mammals examined, the presence of hantaviruses was detected in 4 of them. Hantavirus prevalence was low in Apodemus agrarius (2.6%), the most numerous mammal species, whereas in the remaining 3 positive species (Microtus agrestis, Myodes glareolus, Sorex araneus) this was 12.5–100%. The presence of hantaviruses was detected only in the animals found on area ‘A’ exposed to flooding, and their prevalence was statistically greater compared to area ‘B’ not exposed to flooding (16.7% vs. 0%, p=0.0345). The overall positivity of the examined small mammals population from the areas ‘A’ and ‘B’ was 8.3%. The sequence analysis of the samples positive for hantavirus proved that the amplified products showed 77–86% homology with the L segment sequence of hantavirus Fusong-Mf-731 isolated from Microtus fortis in China. The presented study is the first to demonstrate the occurrence of hantavirus infection in small mammals from eastern Poland, and the first to demonstrate the significant relationship between flooding and the prevalence of hantaviruses in small mammals.

Źródło:

Annals of Agricultural and Environmental Medicine; 2013, 20, 2
1232-1966

Pojawia się w:

Annals of Agricultural and Environmental Medicine

Dostawca treści:

Biblioteka Nauki

/5

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Skocz do pozycji: 64.

Tytuł:

Molecular and serological diagnosis of Borrellia burgdorferi infection among patients with diagnosed erythema migrans

Autorzy:

Kondrusik, M
Grygorczuk, S.
Skotarczak, B.
Wodecka, B.
Rymaszewska, A.
Pancewicz, S.
Zajkowska, J.
Swierzbinska, R.
Hermanowska-Szpakowicz, T.

Powiązania:

https://bibliotekanauki.pl/articles/51856.pdf

Data publikacji:

2007

Wydawca:

Instytut Medycyny Wsi

Tematy:

human disease
erythema migrans phase
patient
diagnosis
ELISA test
polymerase chain reaction
serological diagnosis
Borrelia burgdorferi
borreliosis

Opis:

The aim of the study was to assess the frequency of Borrelia burgdorferi DNA detection in the blood and urine of patients diagnosed with erythema migrans, and compare the results of PCR-based methods with ELISA methodology. The latter was used to detect serum antibodies against Borrelia burgdorferi of the IgM and IgG classes, before and after antibiotic therapy. The study included 86 patients hospitalized in the Department of Infectious Diseases and Neuroinfections in the Medical Academy in Białystok, diagnosed with the erythema migrans phase of Lyme borreliosis. Examinations were carried out twice: the fi rst at the moment of diagnosis (Trial 1), the second after 4 weeks of antibiotic therapy. The study showed that antibiotic therapy in the early phase of borreliosis does not decrease the sensitivity of PCR and that after 4 weeks of therapy (Trial 2), spirochete DNA is still detectable in most patients (45/86). There was no correlation between detectability of spirochete DNA and the presence of antibodies against B. burgdorferi s.l. (assessed by ELISA) during the course of erythema migrans. The largest percentage of positive results in the detection of B. burgdorferi s.l. DNA was observed in patients who simultaneously possessed IgM and IgG antibodies against B. burgdorferi, while the lowest percentage of PCR positive results was among patients with only IgM antibodies.

Źródło:

Annals of Agricultural and Environmental Medicine; 2007, 14, 2
1232-1966

Pojawia się w:

Annals of Agricultural and Environmental Medicine

Dostawca treści:

Biblioteka Nauki

/5

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Skocz do pozycji: 65.

Tytuł:

Evaluation of methods for Erwinia amylovora detection

Autorzy:

Kaluzna, M.
Pulawska, J.
Mikicinski, A.

Powiązania:

https://bibliotekanauki.pl/articles/1986.pdf

Data publikacji:

2013

Wydawca:

Instytut Ogrodnictwa

Tematy:

Erwinia amylovora
fire blight
real-time polymerase chain reaction
LAMP technique
DNA sequence
detection method
disease control

Źródło:

Journal of Horticultural Research; 2013, 21, 2
2300-5009

Pojawia się w:

Journal of Horticultural Research

Dostawca treści:

Biblioteka Nauki

/5

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Skocz do pozycji: 66.

Tytuł:

Reduced expression of AURKA in peripheral blood of breast cancer patients

Autorzy:

Goh, L.P.W.
See, E.U.H.
Chua, K.H.
Lee, P.-C.

Powiązania:

https://bibliotekanauki.pl/articles/81279.pdf

Data publikacji:

2018

Wydawca:

Polska Akademia Nauk. Czytelnia Czasopism PAN

Tematy:

breast cancer
patient
Aurora kinase
gene expression
peripheral blood
cell cycle
quantitative real-time polymerase chain reaction

Źródło:

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2018, 99, 1
0860-7796

Pojawia się w:

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

Dostawca treści:

Biblioteka Nauki

/5

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Skocz do pozycji: 67.

Tytuł:

Length polymorphism of PCR-amplified genomic fragments of the pregnancy-associated glycoprotein [PAG] gene family in the pig and some other domestic and wild mammals

Autorzy:

Szafranska, B
Panasiewicz, G.
Waclawik, A.

Powiązania:

https://bibliotekanauki.pl/articles/2041811.pdf

Data publikacji:

2001

Wydawca:

Polska Akademia Nauk. Czytelnia Czasopism PAN

Tematy:

trophectoderm
pregnancy
pig
polymorphism
mammal
chorion
domestic mammal
polymerase chain reaction
wild mammal
pregnancy-associated glycoprotein gene

Źródło:

Journal of Applied Genetics; 2001, 42, 3; 335-349
1234-1983

Pojawia się w:

Journal of Applied Genetics

Dostawca treści:

Biblioteka Nauki

/5

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Skocz do pozycji: 68.

Tytuł:

Forecasting potato white mold by assessment of ascospores in Iran fields

Autorzy:

Ojaghian, S.
Mirzaei, A.
Ling, W.

Powiązania:

https://bibliotekanauki.pl/articles/66016.pdf

Data publikacji:

2018

Wydawca:

Polska Akademia Nauk. Czytelnia Czasopism PAN

Tematy:

potato
white mould
mould
mold zob.mould
Sclerotinia sclerotiorum
fungi
assessment
ascospore
field
polymerase chain reaction
Iran

Opis:

Potato white mold caused by Sclerotinia sclerotiorum is an important plant disease occurring in many potato-producing areas throughout the world. In this study, a specific diagnostic method was used to detect and quantify S. sclerotiorum ascospores, and its forecasting ability was assessed in potato fields during flowering periods of 2011 to 2014 in Bahar County, Hamedan Province. Using GenEMBL database, a primer pair, HZSCREV and HZSCFOR, was designed and optimized for the pathogen. After testing the sensitivity of primers, DNA was extracted from samples of outdoor Burkard traps from potato fields. A linear association was observed between pathogen DNA and the number of ascospores using the quantitative PCR (qPCR) technique in the presence of SYBR dye. The qPCR could successfully detect DNA amounts representing two S. sclerotiorum ascospores and was not sensitive to a variety of tested fungi such as Botrytis cinerea, Alternaria brassicae, Fusarium solani. In contrast to the amount of rainfall, a direct relationship was found between ascospore numbers and the incidence of potato white mold from 2011 to 2014.

Źródło:

Journal of Plant Protection Research; 2018, 58, 2
1427-4345

Pojawia się w:

Journal of Plant Protection Research

Dostawca treści:

Biblioteka Nauki

/5

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Skocz do pozycji: 69.

Tytuł:

Characteristics of Aureobasidium pullulans (de Bary et Lowenthal) G. Arnaud isolated from apples and pears with symptoms of sooty blotch in Poland
Charakterystyka grzyba Aureobasidium pullulans (de Bary et Lowenthal) G. Arnaud wyizolowanego z jabłek i gruszek z objawami brudnej plamistości w Polsce

Autorzy:

Mirzwa-Mróz, E.
Wińska-Krysiak, M.
Dzięcioł, R.
Miękus, A.

Powiązania:

https://bibliotekanauki.pl/articles/11542902.pdf

Data publikacji:

2014

Wydawca:

Uniwersytet Przyrodniczy w Lublinie. Wydawnictwo Uniwersytetu Przyrodniczego w Lublinie

Tematy:

Aureobasidium pullulans
fungi
isolation
apple
pear
fruit
disease symptom
sooty blotch
Polska
polymerase chain reaction
Gloeodes pomigena

Opis:

Sooty blotch is a disease of apple and pear caused by a complex of fungi that blemish the fruit surface. Results of molecular studies indicated approximately 30 different fungi species associated with this disease. Apples and pears with symptoms of sooty blotch were collected in summer and early autumn 2006–2010 from trees grown in fungicide non-treated orchards and small gardens located in various regions of Poland. Fungi causing sooty blotch were isolated from fruits and the isolates were divided into six groups, according to their morphological characters. Growth of the fungi colonies were tested on different agar media (PDA, CMA, MEA and Czapek). The ITS region of rDNA from 16 isolates from the first group was amplified by PCR technique and one representative sequence of this isolates was used to alignment in Gene Bank. This isolate was identified as Aureobasidium pullulans and isolates from this group were compared with it on the base of morphological features.
Przyczyną brudnej plamistości jabłek i gruszek jest kompleks grzybów powodujących oszpecenie powierzchni skórki owoców. Wyniki badań molekularnych wskazują, że z tą chorobą wiąże się ponad 30 różnych gatunków grzybów. Jabáka i gruszki z widocznymi objawami brudnej plamistości jabłek zbierano z niechronionych chemicznie sadów i ogródków działkowych w różnych regionach Polski. Owoce byáy zbierane latem i wczesną jesienią w latach 2006–2010. Z plam znajdujących się na owocach wyizolowano sprawców brudnej plamistości jabłek i na podstawie charakterystyki cech morfologicznych uzyskanych izolatów grzybów podzielono je na sześć grup. Badano również wzrost i wygląd kolonii grzybów na różnych podłożach (PDA, CMA, MEA i Czapek). Wyizolowano, a następnie zamplifikowano za pomocą techniki PCR z wykorzystaniem starterów ITS1F i ITS4, genomowe DNA 16 izolatów należących do pierwszej grupy. Grzyb ten został zidentyfikowany jako gatunek Aureobasidium pullulans, a jego zsekwencjonowane izolaty stanowiły wzorzec przy identyfikacji pozostałych izolatów zaklasyfikowanych do tej grupy.

Źródło:

Acta Scientiarum Polonorum. Hortorum Cultus; 2014, 13, 3; 13-22
1644-0692

Pojawia się w:

Acta Scientiarum Polonorum. Hortorum Cultus

Dostawca treści:

Biblioteka Nauki

/5

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Skocz do pozycji: 70.

Tytuł:

Dissecting the action of H2O2 after its metabolic formation in chloroplasts

Autorzy:

Jaspert, N.
Balazadeh, S.
Arif, M.
Muller-Rober, B.
Maurino, V.

Powiązania:

https://bibliotekanauki.pl/articles/80107.pdf

Data publikacji:

2013

Wydawca:

Polska Akademia Nauk. Czytelnia Czasopism PAN

Tematy:

conference
reactive oxygen species
toxic cellular metabolite
signalling molecule
Arabidopsis thaliana
glycolate
chloroplast
real-time polymerase chain reaction

Źródło:

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2013, 94, 2
0860-7796

Pojawia się w:

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

Dostawca treści:

Biblioteka Nauki

/5

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Skocz do pozycji: 71.

Tytuł:

Cloning and expression of the two DNA fragments of the I-18 C region of Chironomus tentans. II. The effects of the applied technology

Autorzy:

Borowicz, B P

Powiązania:

https://bibliotekanauki.pl/articles/66157.pdf

Data publikacji:

1998

Wydawca:

Polska Akademia Nauk. Czytelnia Czasopism PAN

Tematy:

promoter system
cloning
I-18 C gene
T7 RNA polymerase
Chironomus tentans
polymerase chain reaction
DNA fragment

Opis:

The chromosomal I-18 C region of Chironomus tentans contains the I-18 C gene. Two different open reading frames (i.e. the ORF I and II) of two different transcripts (i.e. the 1.8 kb and 4.6 kb RNA) of the I-18 C gene of Chironomus tentans were isolated, at the DNA level, by the Polymerase Chain Reaction, then were cloned into the bluescript vector and finally were cloned into the pET-3a vector in order to translate them in T7 RNA polymerase / promoter system of E. coli. It was possible to obtain the ORF I overexpressed. In a case of the ORF II the low molecular weight polypeptide was detected, however it was not overexpressed, but still this polypeptide was strongly visible on the SDS-polyacrylamide gel.
Region chromosomalny I-18 C Chironomus tentans zawiera gen I-18 C. Dwie odrębne otwarte ramy odczytu (tj. ORF I i II) genu I-18 C Chironomus tentans zostały wyizolowane, na poziomie DNA, przy użyciu Reakcji Łańcuchowej Polimerazy (PCR), a następnie zostały wklonowane do wektora blueskrypt i ostatecznie zostały wklonowane do wektora pET-3a w celu ich translacji w systemie promotora T7 RNA polimerazy w E. coli. Uzyskano bardzo dużą ekspresję ORF I. W przypadku ORF II wykryto obecność polipeptydu o niskiej masie cząsteczkowej i chociaż nie uzyskano jego bardzo dużej ekspresji, tym niemniej polipeptyd ten był silnie widoczny w żelu poliakryloamidowym z SDS.

Źródło:

Journal of Plant Protection Research; 1998, 38, 1
1427-4345

Pojawia się w:

Journal of Plant Protection Research

Dostawca treści:

Biblioteka Nauki

/5

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Skocz do pozycji: 72.

Tytuł:

Application of a duplex-PCR for detection of cows' milk in goats' milk

Autorzy:

Kotowicz, M
Adamczyk, E.
Bania, J.

Powiązania:

https://bibliotekanauki.pl/articles/51721.pdf

Data publikacji:

2007

Wydawca:

Instytut Medycyny Wsi

Tematy:

cow milk
human disease
goat milk
food
allergy
polymerase chain reaction
species identification
milk-derived product
detection
milk

Opis:

A duplex-PCR method, with 2 pairs of primers recognizing sequences of mitochondrial D-loop region, was developed to identify cows’ milk in the milk of goats. The PCR was shown to be specifi c and sensitive, enabling the detection of less than 1% of cows’ milk added to the milk of goats. Simultaneous use of a primer pair for goats’ and cows’ mitochondrial DNA fragment prevented false negative results. The method was applied to track the presence of cow DNA in goat milk available on the Polish market. A total of 54 milk samples from 3 Polish (34) and one foreign producer (20) were examined. In 33 samples, cow DNA was detected, while 21 samples, including all of the 20 samples from foreign producers, produced the goat-specific product only.

Źródło:

Annals of Agricultural and Environmental Medicine; 2007, 14, 2
1232-1966

Pojawia się w:

Annals of Agricultural and Environmental Medicine

Dostawca treści:

Biblioteka Nauki

/5

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Skocz do pozycji: 73.

Tytuł:

The prevalence of Ehrlichia canis, Anaplasma platys and Babesia spp. in dogs in Nueva Ecija, Philippines based on multiplex polymerase chain reaction (mPCR) assay

Autorzy:

Corales, J.M.I.
Viloria, V.V.
Venturina, V.M.
Mingala, C.N.

Powiązania:

https://bibliotekanauki.pl/articles/6624.pdf

Data publikacji:

2014

Wydawca:

Polskie Towarzystwo Parazytologiczne

Tematy:

prevalence
Ehrlichia canis
Anaplasma platys
Babesia
dog
tick-borne disease
Nueva Ecija province
Philippines
multiplex polymerase chain reaction

Opis:

The aim of the study was to determine the prevalence of Ehrlichia canis, Anaplasma platys and Babesia spp. in dogs. It describes the practice of veterinarians in detecting tick-borne diseases in Nueva Ecija, Philippines. Seventy blood samples were collected and were subjected to multiplex PCR for the detection of E. canis, Babesia spp. and A. platys. The prevalence of babesiosis is the highest in Cabanatuan City (2/10), while a 10% prevalence (1/10) was observed in Science City of Muñoz, Talavera and Sta. Rosa. E. canis were only detected in Cabanatuan City. However, no anaplasmosis was detected in any area. The prevalence of babesiosis and ehrlichiosis in Nueva Ecija is 7.14% (5/70) and 2.85% (2/70) respectively. In addition, 70% (7/10) of the Nueva Ecija veterinary practitioners encountered cases of suspected ehrlichiosis in their practice. The diagnosis of ehrlichiosis is based primarily on presented clinical signs and complete blood counts, which include a platelet count. Of the 10 respondents, half utilized test kits while 90% interpreted blood samples. Meanwhile, only 60% of the respondents used an ELISA test kit for ehrlichiosis. For some practitioners, the main reason for not utilizing a kit is the high cost. None of the respondents had previously attended cases of suspected anaplasmosis. Only one respondent diagnosed a case of babesiosis by blood smear microscopy.

Źródło:

Annals of Parasitology; 2014, 60, 4
0043-5163

Pojawia się w:

Annals of Parasitology

Dostawca treści:

Biblioteka Nauki

/5

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Skocz do pozycji: 74.

Tytuł:

Analysis of occurrence of virulence genes among Yersinia enterocolitica isolates belonging to different biotypes and serotypes

Autorzy:

Kot, B
Piechota, M.
Jakubczak, A.

Powiązania:

https://bibliotekanauki.pl/articles/32221.pdf

Data publikacji:

2010

Wydawca:

Polska Akademia Nauk. Czytelnia Czasopism PAN

Tematy:

virulence gene
occurrence
Yersinia enterocolitica
isolate
biotype
serotype
polymerase chain reaction
ystB gene
myfA gene
man
pig
isolation

Opis:

The 150 Y. enterocolitica strains isolated from humans and from pigs belonged to biotypes 4 (68.7%), 1A (18.7%) and 2 (4%), or were biochemically untypeable (8.6%). Biotype 4 was comprised of Y. enterocolitica strains representing serotype 0:3, within biotype 1A the strains either belonged to serotypes 0:5 and 0:6 or were untypeable, and biotype 2 was represented by the strains of serotype 0:9. The strains which were biochemically untypeable belonged to serotypes 0:5, 0:6 and 0:3. Among the strains tested there also were those of an unidentified biotype and serotype. Nearly all the strains of biotype 1A represented genotype ystB+myfA+, and few belonged to genotype ystB+. The presence of the ystB gene in the strains of biotype 1A and only occasional occurrence of the gene in the other biotypes makes ystB a distinguishing marker of biotype 1A. The strains of genotype ystA+ail+myfA+yadA+ predominated in biotype 4 (serotype 0:3). The strains of biotype 2 (serotype 0:9) represented genotype ystA+ail+myfA+, and the plasmid yadA gene was detected in some of them. Within the group of biochemically untypeable strains ystB- and myfA-specific PCR products were mainly obtained. The genotypes determined for the tested biotypes and serotypes of Y. enterocolitica, based upon the selected genes of virulence, can be applied as distinguishing markers and indicators of the potential virulence of Y. enterocolitica strains, excluding bioserotyping.

Źródło:

Polish Journal of Veterinary Sciences; 2010, 13, 1; 13-19
1505-1773

Pojawia się w:

Polish Journal of Veterinary Sciences

Dostawca treści:

Biblioteka Nauki

/5

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Skocz do pozycji: 75.

Tytuł:

Preliminary study on the occurrence of Toxoplasma gondii in Ixodes ricinus ticks from north-western Poland with the use of PCR

Autorzy:

Sroka, J
Wojcik-Fatla, A.
Zwolinski, J.
Zajac, V.
Sawczuk, M.
Dutkiewicz, J.

Powiązania:

https://bibliotekanauki.pl/articles/51615.pdf

Data publikacji:

2008

Wydawca:

Instytut Medycyny Wsi

Tematy:

polymerase chain reaction
parasite
genotyping
Polska
tick
toxoplasmosis
Ixodes ricinus
B1 gene
zoonotic disease
occurrence
Toxoplasma gondii

Źródło:

Annals of Agricultural and Environmental Medicine; 2008, 15, 2
1232-1966

Pojawia się w:

Annals of Agricultural and Environmental Medicine

Dostawca treści:

Biblioteka Nauki

/5

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Skocz do pozycji: 76.

Tytuł:

Overexpression of stress-related genes in Cuscuta campestris in response to host defense reactions

Autorzy:

Rezaei, H.
Alamisaeed, K.
Moslemkhani, C.

Powiązania:

https://bibliotekanauki.pl/articles/81031.pdf

Data publikacji:

2017

Wydawca:

Polska Akademia Nauk. Czytelnia Czasopism PAN

Tematy:

Cuscuta campestris
parasitic plant
crop yield
haustorium
RNA extraction
cDNA synthesis
real-time reverse transcription polymerase chain reaction

Źródło:

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2017, 98, 2
0860-7796

Pojawia się w:

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

Dostawca treści:

Biblioteka Nauki

/5

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Skocz do pozycji: 77.

Tytuł:

A 56-year-old man with RT-PCR negative nasopharyngeal swabs with Coronavirus Disease 2019 (COVID-19) Pneumonia

Autorzy:

Dworzańska, A.
Tudrujek-Zdunek, M.
Mosiewicz, J.
Panasiuk, L.
Tomasiewicz, K.

Powiązania:

https://bibliotekanauki.pl/articles/2085530.pdf

Data publikacji:

2020

Wydawca:

Instytut Medycyny Wsi

Tematy:

RT-PCR
pneumonia
Covid-19
Coronavirus Disease 2019
chest computed tomography
real-time-reverse transcription-polymerase chain-reaction

Opis:

Introduction. Diagnostic procedure in Coronavirus Disease 2019 (COVID-19) is based mainly on performing real-time-reverse transcription-polymerase chain-reaction (RT-PCR), which has been accepted as the gold standard method. In some cases, such as mutations of the SARS-CoV-2 genome, variable viral load kinetics or laboratory errors, it can be false-negative. Case report. The case is presented of a 56-year-old man with respiratory tract symptoms, with twice negative results of real-time-reverse transcription-polymerase chain-reaction of nasopharyngeal swabs and positive chest computed tomography, with typical findings for COVID-19 pneumonia. Conclusions. Patients with negative RT-PCR results, but with positive computed tomography findings characteristic for COVID-19, should be treated as well as those infected.

Źródło:

Annals of Agricultural and Environmental Medicine; 2020, 27, 2; 317-318
1232-1966

Pojawia się w:

Annals of Agricultural and Environmental Medicine

Dostawca treści:

Biblioteka Nauki

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Skocz do pozycji: 78.

Tytuł:

DNA polymorphism in locus D1S80 in Poland. DNA profiling and detection of new alleles by heteroduplex formation between alleles of the same size

Autorzy:

Kwiatkowska, J
Dziechciowska, K
Lisiecka, D
Slomski, R

Powiązania:

https://bibliotekanauki.pl/articles/2046677.pdf

Data publikacji:

1997

Wydawca:

Polska Akademia Nauk. Czytelnia Czasopism PAN

Tematy:

DNA sequence
polymorphism
Polska
hybridization
Polish population
heteroduplex formation
DNA polymorphism
same size
polymerase chain reaction
allele
distribution

Opis:

We have analysed allele distribution at the highly polymorphic variable number of tandem repeats (VNTR) locus D1S80 (pMCT118) in the Polish population using the, polymerase chain reaction (PCR) technique. Characteristics of the D1S80 locus makes it a very useful marker for population genetic research, genetic linkage studies and forensic identification of individuals. During our routine application of the D1S80 marker to paternity testing in several cases of homozygosity detected by polyacrylamide gel electrophoresis, heteroduplex formation for alleles 18 and 24 was also observed. Direct sequencing of PCR products revealed that alleles 18 and 24 of locus D1S80 actually represent a mixture composed of different sequences. Our observations indicate that identification of some 18 and 24 VNTR alleles based only on size estimated in electrophoretic analyses could lead to errors in paternity testing and DNA profiling.

Źródło:

Journal of Applied Genetics; 1997, 38, 3; 335-341
1234-1983

Pojawia się w:

Journal of Applied Genetics

Dostawca treści:

Biblioteka Nauki

/5

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Skocz do pozycji: 79.

Tytuł:

PCR, Real-Time PCR and molecular characteristic of Toxoplasma gondii isolates from wild aquatic birds: preliminary analysis

Autorzy:

Lass, A.
Solarczyk, P.
Kavetska, K.
Dzierzba, E.
Werner, A.
Majewska, A.C.

Powiązania:

https://bibliotekanauki.pl/articles/6029.pdf

Data publikacji:

2016

Wydawca:

Polskie Towarzystwo Parazytologiczne

Tematy:

polymerase chain reaction
real-time PCR method
molecular characteristics
Toxoplasma gondii
isolate
parasite
wild animal
bird
aquatic bird

Źródło:

Annals of Parasitology; 2016, 62, Suppl.
0043-5163

Pojawia się w:

Annals of Parasitology

Dostawca treści:

Biblioteka Nauki

/5

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Skocz do pozycji: 80.

Tytuł:

Detection of barley- and wheat-specific forms of Wheat dwarf virus in their vector Psammotettix alienus by duplex PCR assay

Autorzy:

Trzmiel, K.
Klejdysz, T.

Powiązania:

https://bibliotekanauki.pl/articles/66781.pdf

Data publikacji:

2018

Wydawca:

Polska Akademia Nauk. Czytelnia Czasopism PAN

Tematy:

wheat dwarf virus
virus
cereal crop
detection
barley
Psammotettix alienus
Hemiptera
Auchenorrhyncha
Cicadellidae
leafhopper
duplex polymerase chain reaction

Opis:

Wheat dwarf virus (WDV) has been one of the most common viruses on cereal crops in Poland in the last years. This single stranded DNA virus is transmitted by the leafhopper spec, Psammotettix alienus (Dahlb.) in a persistent manner. It induces yellowing and streaking of leaves, dwarfing or even death of infected plants. The presence of barley- and wheat-specific forms of WDV (WDV-B and WDV-W) and their vector were previously reported in the country, however the literature data did not include any information on the infectivity of the vector in Poland. A duplex polymerase chain reaction (PCR) procedure was developed and optimized for simultaneous detection and differentiation of both forms in the vector. Two sets of primers amplify 734 bp and 483 bp specific fragments for WDV-W and WDV-B, respectively. The results were verified by a sequencing method. The studies were carried out on insect samples collected in autumn from four different locations in Greater Poland. The results confirmed the presence of WDV-W in the tested samples. They also suggested the concomitant of both forms of the virus in the vector. Additional studies to determine virus-vector relationships should be undertaken.

Źródło:

Journal of Plant Protection Research; 2018, 58, 1
1427-4345

Pojawia się w:

Journal of Plant Protection Research

Dostawca treści:

Biblioteka Nauki

/5

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Skocz do pozycji: 81.

Tytuł:

The detection of avocado sunblotch viroid in avocado using a real-time reverse transcriptase polymerase chain reaction

Autorzy:

Morey-Leon, G.
Ortega-Ramirez, E.
Julca-Chunga, C.
Santos-Chanta, C.
Graterol-Caldera, L.
Mialhe, E.

Powiązania:

https://bibliotekanauki.pl/articles/80853.pdf

Data publikacji:

2018

Wydawca:

Polska Akademia Nauk. Czytelnia Czasopism PAN

Tematy:

avocado
Persea americana
avocado sunblotch viroid
real-time reverse transcription polymerase chain reaction
nucleotide sequence
RNA extraction
cDNA synthesis

Źródło:

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2018, 99, 2
0860-7796

Pojawia się w:

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

Dostawca treści:

Biblioteka Nauki

/5

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Skocz do pozycji: 82.

Tytuł:

Isolation, cultivation and identification of Borrelia burgdorferi genospecies from Ixodes ricinus from the city of Brno, Czech Republic

Autorzy:

Pejchalova, K
Zakovska, A.
Mejzlikova, M.
Halouzka, J.
Dendis, M.

Powiązania:

https://bibliotekanauki.pl/articles/49816.pdf

Data publikacji:

2007

Wydawca:

Instytut Medycyny Wsi

Tematy:

cultivation
infection
Brno
infection risk
Lyme borreliosis
Czech Republic
isolation
polymerase chain reaction
Borrelia burgdorferi
Ixodes ricinus
tick
identification

Opis:

A total of 305 ticks (21 larvae, 243 nymphs, 19 females and 22 males) were collected by fl agging of vegetation in suburban woods of Pisárky Park (city of Brno) from July to October 2002. The midgut of each tick was dissected out and transferred individually into BSK-H medium. After cultivation, all specimens were examined by dark-fi eld microscopy (DFM) for the presence of borreliae. Out of 305 tick samples, 45 were (14.8%) DFM positive. The following polymerase chain reaction (PCR) then revealed 37 (12.1%) samples positive for the presence of Borrelia burgdorferi sensu lato DNA. All 37 samples were further analysed by restriction fragment length polymorphism (RFLP) method. PCR-RFLP analysis revealed 14 strains of B. afzelii (37.8%), 15 strains of B. garinii (40.5%) and 2 strains of B. burgdorferi sensu stricto (5.4%). Four samples (10.8%) showed a mixed population of these genospecies. Two samples produced atypical RFLP pattern which were detected by sequence analysis as B. valaisiana (5.4%). Isolation attempts resulted in 21 spirochaetal strains (including two stains of B. valaisiana). The results show the diversity of B. burgdorferi s.l. in tick population and refer the risk of infection by pathogenic borreliae in Brno.

Źródło:

Annals of Agricultural and Environmental Medicine; 2007, 14, 1
1232-1966

Pojawia się w:

Annals of Agricultural and Environmental Medicine

Dostawca treści:

Biblioteka Nauki

/5

Artykuł

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Skocz do pozycji: 83.

Tytuł:

The occurrence of Toxoplasma gondii and Borrelia burgdorferi sensu lato in Ixodes ricinus ticks from East Poland with the use of PCR

Autorzy:

Sroka, J
Szymanska, J.
Wojcik-Fatla, A.

Powiązania:

https://bibliotekanauki.pl/articles/51137.pdf

Data publikacji:

2009

Wydawca:

Instytut Medycyny Wsi

Tematy:

Polska
tick
Ixodes ricinus
Toxoplasma gondii
Borrelia burgdorferi
occurrence
polymerase chain reaction
genotyping
B1 gene
human population
infection

Źródło:

Annals of Agricultural and Environmental Medicine; 2009, 16, 2; 313-319
1232-1966

Pojawia się w:

Annals of Agricultural and Environmental Medicine

Dostawca treści:

Biblioteka Nauki

/5

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Skocz do pozycji: 84.

Tytuł:

Application of DNA markers against illegal logging as a new tool for the Forest Guard Service

Autorzy:

Nowakowska, J.A.

Powiązania:

https://bibliotekanauki.pl/articles/38611.pdf

Data publikacji:

2011

Wydawca:

Instytut Badawczy Leśnictwa

Tematy:

application
DNA marker
DNA structure
wood
molecular identification
Forest Guard Service
tree species
determination
DNA profile
polymerase chain reaction

Opis:

DNA markers are currently the most precise tool for forest tree species identification and can be used for comparative analyses of plant material. Molecular diagnosis of evidence and reference material is based on comparing the structure of DNA markers duplicated in the PCR reaction and estimation of the DNA profiles obtained in studied wood samples. For this purpose, the microsatellite DNA markers are the most suitable tool because of their high polymorphism and accurate detection of structural changes in the genome. The analysis of tree stump DNA profiles let avoid timely collection of data such as tree age, diameter, height and thickness, although such a piece of information may advantageous in wood identification process. For each examined tree species, i.e. Pinus sylvestris L., Picea abies (L.) Karst., Quercus robur L. and Q. petraea (Matt.) Liebl., Fagus sylvatica L., Betula pendula L., and Alnus glutinosa L., wood identification was possible via the DNA profiles established on a basis of minimum 4 microsatellite nuclear DNA loci, and at least one cytoplasmatic (mitochondrial or chloroplast) DNA marker. Determination of the DNA profiles provided fast and reliable comparison of genetic similarity between material of evidence (wood, needles, leaves, seeds) and material of reference (tree stumps) in the forest. This was done with high probability (approximately 98– 99%).

Źródło:

Folia Forestalia Polonica. Series A . Forestry; 2011, 53, 2
0071-6677

Pojawia się w:

Folia Forestalia Polonica. Series A . Forestry

Dostawca treści:

Biblioteka Nauki

/5

Artykuł

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Skocz do pozycji: 85.

Tytuł:

What is percentage of pathogenic borreliae in spirochaetal findings of mosquito larvae?

Autorzy:

Nejedla, P
Norek, A.
Vostal, K.
Zakovska, A.

Powiązania:

https://bibliotekanauki.pl/articles/51950.pdf

Data publikacji:

2009

Wydawca:

Instytut Medycyny Wsi

Tematy:

mosquito
larva
Culex
spirochaete
polymerase chain reaction
Borrelia burgdorferi
Culex pipiens
human disease
tick
Ixodes ricinus
midgut
Czech Republic

Źródło:

Annals of Agricultural and Environmental Medicine; 2009, 16, 2; 273-276
1232-1966

Pojawia się w:

Annals of Agricultural and Environmental Medicine

Dostawca treści:

Biblioteka Nauki

/5

Artykuł

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Skocz do pozycji: 86.

Tytuł:

Molecular characterization of early stages of ovule embryogenesis in sugar beet (Beta vulgaris L.)

Autorzy:

Cichorz, S.
Malicka, M.
Goska, M.

Powiązania:

https://bibliotekanauki.pl/articles/80424.pdf

Data publikacji:

2013

Wydawca:

Polska Akademia Nauk. Czytelnia Czasopism PAN

Tematy:

conference
embryogenesis
sugar-beet
Beta vulgaris
donor plant
plant genotype
haploid induction
androgenesis
molecular mechanism
gynogenesis
polymerase chain reaction

Źródło:

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2013, 94, 3
0860-7796

Pojawia się w:

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

Dostawca treści:

Biblioteka Nauki

/5

Artykuł

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Skocz do pozycji: 87.

Tytuł:

In vitro and molecular characterization using ISSR markers of Glycyrrhiza glabra L.

Autorzy:

El-Hameid, A.A.
El-Kheir, Z.A.
Abdel-Hady, M.
Helmy, W.

Powiązania:

https://bibliotekanauki.pl/articles/80908.pdf

Data publikacji:

2018

Wydawca:

Polska Akademia Nauk. Czytelnia Czasopism PAN

Tematy:

licorice
Glycyrrhiza glabra
callus induction
genomic DNA
ISSR marker
molecular characteristics
polymerase chain reaction
kinetin
Murashige medium
Skoog's medium

Źródło:

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2018, 99, 4
0860-7796

Pojawia się w:

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

Dostawca treści:

Biblioteka Nauki

/5

Artykuł

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Skocz do pozycji: 88.

Tytuł:

Association between beta-lactoglobulin (LGB) polymorphism and yield and composition of milk Holstein-Friesian cows imported from Sweden
Związek polimorfizmu beta-laktoglobuliny (LGB) z wydajnością i składem mleka krów rasy holsztyńsko-fryzyjskiej importowanych ze Szwecji

Autorzy:

Czerniawska-Piatkowska, E.
Szewczuk, M.
Olszewska, A.
Chocilowicz, E.

Powiązania:

https://bibliotekanauki.pl/articles/45040.pdf

Data publikacji:

2011

Wydawca:

Zachodniopomorski Uniwersytet Technologiczny w Szczecinie. Wydawnictwo Uczelniane ZUT w Szczecinie

Tematy:

beta-lactoglobulin
polymorphism
milk yield
milk composition
milk protein
genotype
polymerase chain reaction
Holstein-Friesian breed
cow
import
Sweden

Opis:

The study involved 102 Holstein-Friesian cows imported from Sweden, kept on a farm in the West Pomerania Province. Beta-lactoglobulin (LGB) genotypes were determined using PCR (Polymerase Chain Reaction) method according to Medrano andAquilar-Cordova [1990]. It was found that cows with the LGB AA genotype had the highest milk yield in all analysed 305-day lactations. The differences were significant in the third lactation (P≤0.01, P≤0.05). For the same genotype, the highest milk protein yield and content for the three analysed lactations were recorded. The highest milk fat content and yield were found in the BB homozygotes for all the analysed lactations.
Badania przeprowadzono na 102 krowach rasy holsztyńsko-fryzyjskiej importowanych ze Szwecji, w gospodarstwie rolnym w województwie zachodniopomorskim. Genotypy beta-laktoglobuliny (LGB) oznaczono metodą PCR (ang. Polymerase Chain Reaction) wg metodyki Medrano i Aquilar-Cordova [1990]. Stwierdzono, że krowy o genotypie LGB AA uzyskały najwyższą wydajność mleka we wszystkich analizowanych 305-dniowych laktacjach. W trzeciej laktacji różnice były istotne (P≤0,01; P≤0,05). Dla tego samego genotypu zanotowano najwyższą wydajność i zawartość białka w mleku dla trzech analizowanych laktacji. Najwyższą zawartość i wydajność tłuszczu stwierdzono u homozygot BB dla wszystkich badanych laktacji.

Źródło:

Acta Scientiarum Polonorum. Zootechnica; 2011, 10, 2
1644-0714

Pojawia się w:

Acta Scientiarum Polonorum. Zootechnica

Dostawca treści:

Biblioteka Nauki

/5

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Skocz do pozycji: 89.

Tytuł:

Detection of Narcissus latent virus isolates using one-step RT-PCR assay

Autorzy:

Berniak, H.
Komorowska, B.
Sochacki, D.

Powiązania:

https://bibliotekanauki.pl/articles/1948.pdf

Data publikacji:

2013

Wydawca:

Instytut Ogrodnictwa

Tematy:

narcissus latent virus
virus identification
reverse transcription polymerase chain reaction
DAS-ELISA method
RNA sequence
cucumber mosaic virus
polyclonal antibody

Źródło:

Journal of Horticultural Research; 2013, 21, 1
2300-5009

Pojawia się w:

Journal of Horticultural Research

Dostawca treści:

Biblioteka Nauki

/5

Artykuł

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Skocz do pozycji: 90.

Tytuł:

Molecular characterization of ‘Candidatus Phytoplasma mali’ strains from Bulgaria and Poland

Autorzy:

Cieślińska, M.
Borisova, A.

Powiązania:

https://bibliotekanauki.pl/articles/12308151.pdf

Data publikacji:

2019

Wydawca:

Uniwersytet Przyrodniczy w Lublinie. Wydawnictwo Uniwersytetu Przyrodniczego w Lublinie

Tematy:

Polska
Bulgaria
apple proliferation phytoplasma
molecular characteristics
Candidatus Phytoplasma mali
polymerase chain reaction
RFLP method
multilocus gene analysis
genetic diversity

Opis:

During 2015, samples from 22 apple trees showing proliferation symptoms were collected in southwest Bulgaria and Central and South Poland and tested for phytoplasma presence. ‘Candidatus Phytoplasma mali’ was identified in 18 samples based on results of restriction fragment length polymorphism (RFLP) analysis of the 16S rRNA gene amplified in nested PCR using primer pair P1/P7 followed by R16F2n/R16R2 and F1/B6 primer pairs. The nitroreductase and rhodonase-like genes and ribosomal protein genes rpl22 and rps3 were then analyzed using PCR-RFLP technique to study the genetic variability of the phytoplasma strains. Two restriction profiles, P-I or P-II, were obtained from fragments of 16S rDNA plus 16S-23S spacer region digested with HpaII enzyme. Restriction fragment length polymorphism analysis of nitroreductase and rhodonase-like genes using digestion with HincII endonuclease revealed that all ‘Ca. P. mali’ strains belonged to the subtype AP-15. Analysis of rpl22 and rps3 ribosomal protein genes digested with AluI enzyme resulted in classification of detected phytoplasma strains to rpX-A subgroup.

Źródło:

Acta Scientiarum Polonorum. Hortorum Cultus; 2019, 18, 5; 181-188
1644-0692

Pojawia się w:

Acta Scientiarum Polonorum. Hortorum Cultus

Dostawca treści:

Biblioteka Nauki

/5

Artykuł

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Skocz do pozycji: 91.

Tytuł:

The effect of water stress on the gas exchange parameters, productivity and seed health of buckwheat (Fagopyrum esculentum Moench)
Wpływ stresu wodnego na wskaźniki wymiany gazowej, produktywność i zawartość grzybni (Fagopyrum esculentum)

Autorzy:

Pszczolkowska, A.
Fordonski, G.
Kulik, T.
Olszewski, J.
Plodzien, K.
Lojko, M.

Powiązania:

https://bibliotekanauki.pl/articles/28298.pdf

Data publikacji:

2010

Wydawca:

Polskie Towarzystwo Botaniczne

Tematy:

water stress
gas exchange
gas exchange parameter
productivity
seed health
buckwheat
Fagopyrum esculentum
polymerase chain reaction
water deficit
protein content

Opis:

The present pot experiment studied the effect of different soil moisture contents (60 – 70% CWC (capillary water capacity) – control; 30 – 35% CWC – water stress) on buckwheat productivity, the gas exchange parameters and health of buckwheat nuts. It was found that water deficit affected adversely certain biometric features investigated (plant height, number of nuts per cluster) and caused a decrease in seed weight per plant. It was also shown that water stress reduced the values of the investigated gas exchange parameters (photosynthesis rate, transpiration rate, intercellular-space CO2 concentration, and stomatal conductance) relative to the control treatment. Different soil moisture contents did not have a clear effect on fungal colonization of seeds. The multiplex PCR assays did not enable the detection of the genes responsible for mycotoxin synthesis. Under water deficit conditions, an increase was found in the content of albumin and globulin fractions as well as of glutelin fractions.

Źródło:

Acta Agrobotanica; 2010, 63, 1
0065-0951
2300-357X

Pojawia się w:

Acta Agrobotanica

Dostawca treści:

Biblioteka Nauki

/5

Artykuł

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Skocz do pozycji: 92.

Tytuł:

Results of study over Ehrlichia canis appearing in ticks infesting dogs in Lower Silesian Region

Autorzy:

Ploneczka, K.

Powiązania:

https://bibliotekanauki.pl/articles/838266.pdf

Data publikacji:

2007

Wydawca:

Polskie Towarzystwo Parazytologiczne

Tematy:

parasite
parasitic disease
Ehrlichia canis
ehrlichiosis
dog
transmission
canine monocytic ehrlichiosis
polymerase chain reaction
tick-borne disease
animal disease
tick

Źródło:

Annals of Parasitology; 2007, 53, 1
0043-5163

Pojawia się w:

Annals of Parasitology

Dostawca treści:

Biblioteka Nauki

/5

Artykuł

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Skocz do pozycji: 93.

Tytuł:

Characterization of phytoplasmas related to ‘Candidatus Phytoplasma asteris’ subgroup rpI-L in Iran

Autorzy:

Vali-Sichani, F.
Bahar, M.
Zirak, L.

Powiązania:

https://bibliotekanauki.pl/articles/66181.pdf

Data publikacji:

2014

Wydawca:

Polska Akademia Nauk. Czytelnia Czasopism PAN

Tematy:

phytoplasma
disease symptom
plant
disease
polymerase chain reaction
seed
Candidatus Phytoplasma asteris
rpI-L subgroup
rp gene
tuf gene
Iran

Opis:

In two of Iran's central provinces, several herbaceous plants showing phytoplasma disease symptoms were collected to detect 'Canididatus Phytoplasma asteris'-related phytoplasmas. Confirmation of an association of phytoplasmas with diseased plants was done using polymerase chain reaction (PCR) assays having the phytoplasma universal primer pairs P1/P7 followed by R16F2n/ R16R2 in nested PCR. Then, for detection of 'Ca. P. asteris', DNA samples were subjected to amplification of rp and tuf genes using specific primer pairs rp(I)F1A/rp(I)R1A and fTufAy/rTufAy, respectively. Restriction fragment length polymorphism or RFLP analyses of rp gene fragments using Tsp509I restriction enzyme as well as sequence analyses indicated that 'Ca. P. asteris'-related phytoplasmas associated with carrot, niger seed and scallion plants in these regions, belong to the rpI-L subgroup. This research is the first report of carrot, niger seed, and scallion infection with phytoplasmas belonging to the rpI-L subgroup.

Źródło:

Journal of Plant Protection Research; 2014, 54, 2
1427-4345

Pojawia się w:

Journal of Plant Protection Research

Dostawca treści:

Biblioteka Nauki

/5

Artykuł

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Skocz do pozycji: 94.

Tytuł:

PCR for identification and typing of Helicobacter pylori isolated from children

Autorzy:

Dzierzanowska, D
Gzyl, A.
Rozynek, E.
Augustynowicz, E.
Wojda, U.
Celinska-Cedro, D.
Sankowska, M.
Wadstrom, T.

Powiązania:

https://bibliotekanauki.pl/articles/69030.pdf

Data publikacji:

1996

Wydawca:

Polskie Towarzystwo Fizjologiczne

Tematy:

antibiotic therapy
infection
Chlamydia
Mycobacterium
Helicobacter pylori
antibody
antigen
virus
gastroduodenal disease
microorganism
child
protein
polymerase chain reaction
Legionella
DNA

Źródło:

Journal of Physiology and Pharmacology; 1996, 47, 1
0867-5910

Pojawia się w:

Journal of Physiology and Pharmacology

Dostawca treści:

Biblioteka Nauki

/5

Artykuł

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Skocz do pozycji: 95.

Tytuł:

Results of study over Ehrlichia canis appearing in ticks infesting dogs in Lower Silesian Region

Autorzy:

Płoneczka, K.

Powiązania:

https://bibliotekanauki.pl/articles/2143846.pdf

Data publikacji:

2007

Wydawca:

Polskie Towarzystwo Parazytologiczne

Tematy:

parasite
parasitic disease
Ehrlichia canis
ehrlichiosis
dog
transmission
canine monocytic ehrlichiosis
polymerase chain reaction
tick-borne disease
animal disease
tick

Opis:

Ehrichia canis is an etiologic agent of canine monocytic ehrlichiosis — usually asymptomatic in early stage of disease. It is a tick−borne disease. The aim our study was to estimate the prevalence of ricketsia in the tick population, that infested dogs in Lower Silesia region during season 2005 by PCR. The study took to consideration of identification of ticks species and possibility of season−influence on appear of infections. Among collected ticks (n=102), 97% represented Ixodes ricinus. Other species infesting dogs were Ixodes hexagonus (n=1) and Hemaphysalis punctata (n=2). All examined ticks were negative in the PCR reaction of Ehrlichia canis. Because of negative results there was no possibility to establish season−influence of appearing ricketsia infections.

Źródło:

Wiadomości Parazytologiczne; 2007, 53, 1; 45-46
0043-5163

Pojawia się w:

Wiadomości Parazytologiczne

Dostawca treści:

Biblioteka Nauki

/5

Artykuł

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Skocz do pozycji: 96.

Tytuł:

Serological and molecular survey of Anaplasma phagocytophilum in Italian hunting dogs

Autorzy:

Ebani, V.V.
Bertelloni, F.
Turchi, B.
Cerri, D.

Powiązania:

https://bibliotekanauki.pl/articles/50930.pdf

Data publikacji:

2013

Wydawca:

Instytut Medycyny Wsi

Tematy:

serological survey
molecular survey
Anaplasma phagocytophilum
hunting dog
dog
polymerase chain reaction
indirect immunofluorescence antibody test
tick
zoonotic pathogen
Italy
prevalence

Opis:

The presence of Anaplasma phagocytophilum, a tick-transmitted zoonotic pathogen, has been reported in Italy in humans and several animal species, including dogs, but data concerning its prevalence in the Italian canine population are lacking. The aim of this study was to determine the prevalence of A. phagocytophilum infection in hunting dogs which live in geographic areas of central Italy where the infection had been previously detected in wild animals. Sera from 215 hunting dogs were tested by indirect immunofluorescence antibody test (IFAT) to detect antibodies to A. phagocytophilum. Buffy coat samples from the same 215 animals were submitted to a polymerase chain reaction (PCR) assay specific for the A. phagocytophilum 16S rRNA gene. Thirty-two (14.8%) dogs proved to be seropositive with antibody titres from 1:40–1:2,560. The highest seroprevalence was observed in dogs 6–10-year-old. Two seropositive (0.9%) animals were also PCR positive. Sequencing of PCR products revealed gene sequences of A. phagocytophilum in both cases. These results confirm the presence of A. phagocytophilum in the Italian areas studied; thus, the zoonotic potential of this agent should be considered particularly for people, as hunters, at risk of exposure to tick bites.

Źródło:

Annals of Agricultural and Environmental Medicine; 2013, 20, 2
1232-1966

Pojawia się w:

Annals of Agricultural and Environmental Medicine

Dostawca treści:

Biblioteka Nauki

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Skocz do pozycji: 97.

Tytuł:

Wykrywanie bakterii Salmonella metodami molekularnymi w produktach żywnościowych
Detection of Salmonella sp. in food using molecular methods

Autorzy:

Misiewicz, A.
Goncerzewicz, A.

Powiązania:

https://bibliotekanauki.pl/articles/796639.pdf

Data publikacji:

2013

Wydawca:

Szkoła Główna Gospodarstwa Wiejskiego w Warszawie. Wydawnictwo Szkoły Głównej Gospodarstwa Wiejskiego w Warszawie

Tematy:

produkty spozywcze
bakterie
Salmonella
wykrywanie drobnoustrojow
metody molekularne
lancuchowa reakcja polimerazy
food product
bacteria
microorganism detection
molecular method
polymerase chain reaction

Opis:

Bakterie z rodzaju Salmonella są najbardziej znanymi bakteriami patogennymi występującymi w przemyśle spożywczym. Powodują zakażenia prawie wszystkich produktów żywnościowych – od mięsnych, mlecznych, jajecznych po rośliny oleiste i pasze. Wykrycie i identyfi kacja bakterii Salmonella na podstawie tradycyjnej metody mikrobiologicznej, zgodnie z normą PN-EN 6579:2003, jest ciągle powszechnie stosowane w laboratoriach. Analiza ta jest czaso- i pracochłonna, jej wykonanie trwa około 5–7 dni. Wykorzystanie nowoczesnych technik biologii molekularnej, z etapem namnożenia i izolacji DNA, do uzyskania końcowego wyniku trwa znacznie krócej. W niniejszej pracy zastosowano metodę Real-Time PCR i klasyczny PCR do identyfi kacji bakterii Salmonella w różnych produktach żywnościowych. Określono czas potrzebny do wykonania tej analizy molekularnej. Do reakcji Real-Time PCR wykorzystano komercyjny kit. Klasyczną reakcję PCR prowadzono z użyciem starterów Sal465Li Sal142F, uzyskując właściwy produkt o wielkości 343 pz. We wszystkich badanych próbkach wykryto bakterie Salmonella. Wynik analiz molekularnych uzyskano w ciągu 21–25 godzin.
Pathogenic bacteria of Salmonella genus are the most common infections in food industry. They might to contaminate wide range of products, protein food e.g. meat, milk food, eggs and egg foods, plants and its preserves, fodder and feeds. Detection and identifi cation of Salmonella sp. are made using traditional methods corresponding with standard PN-EN 6579:2003. That method is commonly used in the microbiological laboratories its time consuming and laborious analysis. Using a modern molecular biology techniques allow to confi rm the Salmonella infections in 24-hours with enrichment and isolation steps. In our study were used Real-Time PCR and traditional PCR techniques to detect Salmonella pathogens from various foods. In addition we checked time of molecular analysis. In the study was used commercial kit to Real-Time PCR analysis and primer set Sal465L, Sal142F with are based on characteristic and solid genomic fragments of Salmonella genus. In every experiment we got positive results for food contaminated by Salmonella. The results were obtained in 21–25 hours.

Źródło:

Zeszyty Problemowe Postępów Nauk Rolniczych; 2013, 573
0084-5477

Pojawia się w:

Zeszyty Problemowe Postępów Nauk Rolniczych

Dostawca treści:

Biblioteka Nauki

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Skocz do pozycji: 98.

Tytuł:

The first description of gastric Helicobacter in free-ranging wild boar [Sus scrofa] from Poland

Autorzy:

Fabisiak, M
Sapierzynski, R.
Salamaszynska-Guz, A.
Kizerwetter-Swida, M.

Powiązania:

https://bibliotekanauki.pl/articles/32325.pdf

Data publikacji:

2010

Wydawca:

Polska Akademia Nauk. Czytelnia Czasopism PAN

Tematy:

boar
wild animal
gastric mucosa
Sus scrofa
Polska
hunting season
Helicobacter
histopathology
polymerase chain reaction
Helicobacter pylori
animal species
animal infection

Opis:

Specimens of gastric mucosa of 17 free-ranging wild boars (Sus scrofa) shot in the Central Poland during 2007/2008 hunting season were investigated for the presence of Helicobacter species. Histopathology, Helicobacter genus-specific 16S rRNA PCR, and DNA sequence analysis were employed. In PCR analysis the presence of Helicobacter's DNA was detected in one stomach. Obtained sequence analysis showed its relatedness to Helicobacter heilmannii type 2. In histopathology of the PCR-positive sample the presence of tightly coiled spiral bacteria was detected on the surface of the antral mucosa, in gastric pits and lumen of the upper parts of antral glands. Potential pathologic significance of the presence of Helicobacter in the stomach of free-ranging wild boars was obscured by the parasitic invasion-caused gastritis, and remains unknown.

Źródło:

Polish Journal of Veterinary Sciences; 2010, 13, 1; 171-174
1505-1773

Pojawia się w:

Polish Journal of Veterinary Sciences

Dostawca treści:

Biblioteka Nauki

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Skocz do pozycji: 99.

Tytuł:

Isolation and preliminary sequence characterization of beta-amylase gene promoters in rye [Secale cereale L.]

Autorzy:

Sadowski, J
Rorat, T
Cooke, R
Delseny, M

Powiązania:

https://bibliotekanauki.pl/articles/2046684.pdf

Data publikacji:

1997

Wydawca:

Polska Akademia Nauk. Czytelnia Czasopism PAN

Tematy:

beta-amylase
rye
gene promoter
homology
amino acid
DNA cloning
prolamin
Secale cereale
mRNA
embryogenesis
endosperm
cDNA
inverse polymerase chain reaction

Opis:

The isolation of rye ß-Amy1 and ß-Amy2 gene promoters from nuclear DNA using the inverse polymerase chain reaction (IPCR) technique and characterization of their sequences are presented. The conservation of ß-amylasc coding sequences allowed for simultaneous IPCR amplification of two different promoters with primers designed on the basis of the single known cDNA sequence. Two ß-amylasc gene promoters display a low sequence similarity (47%). Beside consensus TATA and CCAAT boxes, other sequence motives common to both promoters were found. In addition, the homology of amino acid sequences of plant ß-amylases available in the database is discussed.

Źródło:

Journal of Applied Genetics; 1997, 38, 3; 241-251
1234-1983

Pojawia się w:

Journal of Applied Genetics

Dostawca treści:

Biblioteka Nauki

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Skocz do pozycji: 100.

Tytuł:

Cacao swollen shoot virus detection and DNA barcoding of its vectors and putative vectors in Theobroma cacao L. by using polymerase chain reaction

Autorzy:

Obok, E.E.
Aikpokpodion, P.O.
Ani, O.C.
Allainguillaume, J.
Wetten, A.

Powiązania:

https://bibliotekanauki.pl/articles/2096411.pdf

Data publikacji:

2021

Wydawca:

Polska Akademia Nauk. Czytelnia Czasopism PAN

Tematy:

Cacao swollen shoot virus
COI – cytochrome c oxidase subunit I
DNA barcoding
Jack Beardsley
mealybug
PCR – polymerase chain reaction
Theobroma cacao

Opis:

Cacao swollen shoot virus (CSSV) is an endemic pathogen causing significant economic losses to cacao (Theobroma cacao L.) production in West Africa. There is limited updated report on the occurrence, spread, genetic diversity and species of CSSV and its mealybug vectors, especially in Nigeria. Nigeria is presently lagging behind in the search for resistance to CSSV and its vectors in T. cacao L. The present study aimed to map and screen for the presence of CSSV and its natural vectors – female mealybugs (Pseudococcidae: Hemiptera) in cacao plantations in Nigeria. Symptomatic and asymptomatic cacao leaves and whole female mealybug samples were collected from major cacao-growing areas in Nigeria – Abia, Akwa Ibom, Cross River, Edo, Ondo and Oyo States. A total of 2568 cacao leaves from 1052 cacao trees were screened with polymerase chain reaction (PCR) using an open reading frame 1 (ORF 1) CSSV-specific primer pair. PCR screening of the mealybug species was performed using the cytochrome c oxidase subunit I (COI) gene. A combination of scanning electron microscopy (SEM) and histology for morphological identification and DNA barcoding enabled to characterise the female mealybug species. The results revealed that CSSV and its mealybug vectors are present in the major cacao-growing areas in Nigeria. Although CSSV and its vectors have been previously reported in Cross River, Ondo and Oyo States, our results present the first documented evidence of CSSV emergence and its mealybug vectors in Abia, Akwa Ibom and Edo States. We also present the first report of Pseudococcus jackbeardsleyi (Gimpel and Miller) mealybug species on cacao in Nigeria. In conclusion, it is pertinent to re-establish coordinated routine survey and monitoring of CSSV and its mealybug vector presence in T. cacao L. in Nigeria.

Źródło:

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2021, 102, 3; 229-244
0860-7796

Pojawia się w:

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

Dostawca treści:

Biblioteka Nauki

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