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Tytuł:
Determination of Pleurotus abieticola ligninolytic activity on norway spruce wood
Autorzy:
Żółciak, Anna
Powiązania:
https://bibliotekanauki.pl/articles/2041965.pdf
Data publikacji:
2019
Wydawca:
Instytut Badawczy Leśnictwa
Tematy:
laccase
lignin peroxidase
manganese peroxidase
versatile peroxidase
spruce
white rot fungus
wood
Opis:
The effect of Phlebiopsis gigantea treatment in control of Heterobasidion parviporum in Norway spruce is less effective than that in control of Heterobasidion annosum in pine. It is necessary to apply other fungi, for example, Pleurotus abieticola in Norway spruce stands. Thus, it is necessary to assess the activity of major ligninolytic enzymes, that is, laccase, lignin peroxidase (LiP), manganese peroxidase (MnP) and versatile peroxidase (VP) produced by P. abieticola, which may be effective in the fast degradation of Norway spruce wood. Three strains of P. abieticola (Pa1-3) were grown on pieces of Norway spruce sapwood and heartwood for 50 days in laboratory conditions. Enzymatic activity was determined using spectrophotometry. Pleurotus abieticola produced laccase, LiP, MnP and VP. The activity of laccase was low, ranging 0–3.696 and 0–0.806 mU/μg of protein in sapwood and heartwood, respectively. The highest activity in Pa1 = 3.696 mU/μg of protein in sapwood and in Pa3 = 0.806 mU/μg of protein in heartwood was observed after 30 and 50 days of incubation, respectively. The activity of LiP was also low, ranging 0–0.188 and 0–0.271 mU/μg of protein in sapwood and heartwood, respectively. The highest activity in Pa1 = 0.271 mU/μg of protein in sapwood and in Pa2 = 0.188 mU/μg of protein in heartwood was observed after 40 and 20 days of incubation, respectively. The activity of MnP ranged 0–17.618 and 0–12.203 mU/μg of protein in sapwood and heartwood, respectively. This enzymatic activity peaked at the 50th day of culture on sapwood for the Pa3 strain (17.618 mU/μg of protein) and at the 20th day of culture on heartwood for the Pa1 strain (12.203 mU/μg of protein). The activity of VP with manganese-oxidising properties was found to be high in all strains of P. abieticola, ranging 0–39.19 and 0–59.153 mU/μg of protein in sapwood and heartwood, respectively, whereas the activity of VP with guaiacol-oxidising properties was very low for all P. abieticola strains, ranging 0–0.248 and 0–0.225 mU/μg of protein in sapwood and heartwood, respectively. The values of released hydroxyphenols in P. abieticola strains ranged 24.915–139.766 and 25.19–84.562 µg of protocatechuic acid/ml in sapwood and heartwood, respectively. The values of released methoxyphenols for the evaluated strains of P. abieticola ranged 7.225–23.789 and 1.953–20.651 µg of vanillic acid/ml in sapwood and heartwood, respectively. Further studies with a higher number of strains of this species as well as an optimisation of conditions for the measurement of ligninolytic activity are needed.
Źródło:
Folia Forestalia Polonica. Series A . Forestry; 2019, 61, 4; 267-277
0071-6677
Pojawia się w:
Folia Forestalia Polonica. Series A . Forestry
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Intraspecific differentiation of reed grass Calamagrostis arundinacea [L.] Roth [Poaceae] populations revealed by peroxidase allozymes
Autorzy:
Krzakowa, M
Celka, Z.
Powiązania:
https://bibliotekanauki.pl/articles/58313.pdf
Data publikacji:
2008
Wydawca:
Polskie Towarzystwo Botaniczne
Tematy:
intraspecific differentiation
reed grass
Calamagrostis arundinacea
Poaceae
plant population
peroxidase allozyme
natural population
Polska
peroxidase
geographic region
Opis:
The genetic Variation of Reed Grass Calamagrostis arundinacea (L.) Roth was investigated in 25 populations in various geographic regions of Poland. A total of 907 individuals were sampled for electrophoretic analysis of peroxidase loci (11 allozymes). Populations were characterised by genetic parameters e.g. heterozygosity level, Wright's fixation index (F) and polymorphism coefficient (Pg). Mean values of interpopulation variability level (GST=0.0310), total genetic diversity (HT=0.4102) and gene flow between populations (Nm=7.805) were also examined. All the populations were polymorphic and they remain in Hardy Weinberg equilibrium.
Źródło:
Acta Societatis Botanicorum Poloniae; 2008, 77, 4; 299-304
0001-6977
2083-9480
Pojawia się w:
Acta Societatis Botanicorum Poloniae
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Kinetic studies on the oxidation of nitrite by horseradish peroxidase and lactoperoxidase
Autorzy:
Gębicka, Lidia
Powiązania:
https://bibliotekanauki.pl/articles/1044450.pdf
Data publikacji:
1999
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
lactoperoxidase
nitrite
stopped-flow
horseradish peroxidase
Opis:
The reaction of nitrite (NO-_2) with horseradish peroxidase and lactoperoxidase was studied. Sequential mixing sopped-flow measeruments gave the following values for the rate constants of the reaction of nitrite with compounds II (oxoferryl heme intermediates) of horseradish peroxidase and lactoperoxidase at pH 7.0, 13.3 ± 0.07 mol^{-1}dm^3s^{-1} and 3.5 ± 0.05 · 10^4mol^{-1}dm^3s^{-1}, respectively. Nitrite, at neutral pH, influenced measurements of activity of lactoperoxidase with typical substrates like 2,2'-azino-bis[ethyl-benzothiazoline-(6)-sulphonic acid] (ABTS), guaiacol or thiocyanate (SCN-). The rate of ABTS and guaiacol oxidation increased linearly with nitrite concentration up to 2.5-5 mmol dm^{-3}. On the other hand, two-electron SCN- oxidation was inhibited in the presence od nitrite. Thus, nitrite competed with the investigated substrates of lactoperoxidase. The intermediate, most probably nitrogen dioxide (*NO_2), reacted more rapidly with ABTS or guaiacol than did lactoperoxidase compound II. It did not, however, effectively oxidize SCN- to OSCN-. NO-_2 did not influence the activity measurements of horseradish peroxidase by ABTS or guaiacol method.
Źródło:
Acta Biochimica Polonica; 1999, 46, 4; 919-927
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Peroxidase and IAA oxidase activities during sink development in cotton seed.
Autorzy:
Rabadia, Vraj
Thaker, Vrinda
Singh, YeshDev
Powiązania:
https://bibliotekanauki.pl/articles/2199024.pdf
Data publikacji:
2006-06-22
Wydawca:
Instytut Hodowli i Aklimatyzacji Roślin
Tematy:
cotton
IAA oxidase
peroxidase
seed development
Opis:
Seeds of the three cotton genotypes, H-6 and H-4 (Gossypium hirsutum),and V797 (G. herbaceum) for their growth variations in IAA oxidase and peroxidase activities. The three genotypes varied in their final seed weight. The peroxidase analysis was done with four different hydrogen donors. Though the trends in peroxidase activitywere almost similar in the three genotypes, there was variation with different hydrogen donors. These was an inverse correlation between Cytoplasmic peroxidase and Wall bound peroxidase activity and rate of water uptake of the seed. The peroxidase activity showed up increasing levels only after the rate of water uptake decreased. However, IAA oxidase activity did not show any discernible trend with water content. The role of cytoplasmic and wall bound peroxidase in seed development is discussed in detail    
Źródło:
Plant Breeding and Seed Science; 2006, 53; 27-36
1429-3862
2083-599X
Pojawia się w:
Plant Breeding and Seed Science
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Controllable preparation of highly active horseradish peroxidase-gold nanoparticle bionanoconjugate
Autorzy:
Zhang, P.
Liu, Ch.
Song, S.
Peng, Ch.
Powiązania:
https://bibliotekanauki.pl/articles/778931.pdf
Data publikacji:
2012
Wydawca:
Zachodniopomorski Uniwersytet Technologiczny w Szczecinie. Wydawnictwo Uczelniane ZUT w Szczecinie
Tematy:
horseradish peroxidase
gold nanoparticles
bionanoconjugate
Opis:
A novel method of immobilizing horse radish peroxidase enzyme (HRP) onto the surface of gold nanoparticles (GNPs) was developed. As a result, a high-activity bionanoconjugates was obtained through utilizing the biotin-streptavidin (SA) system. The HRP-SA-GNP bionanoconjugate with high activity was conveniently prepared through the biotin- avidin system. Compared with the HRP-GNP bioconjugate prepared through the traditional electrostatic absorption method, the enzyme activity per GNPs of this new bionanoconjugate was enhanced by 10 times. Moreover, the enzyme activity of this bionanoconjugate was controllable. The above method of bionanoconjugation preparation has promising applications In the fi elds including preparing highly active bio-nanoprobe and immobilized enzyme.
Źródło:
Polish Journal of Chemical Technology; 2012, 14, 4; 57-60
1509-8117
1899-4741
Pojawia się w:
Polish Journal of Chemical Technology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Enzymatic assay of opioid analgesic, tramadol, using horseradish peroxidase
Autorzy:
Sridevi, N.
Srilakshmi, C.
Alekya, G.
Bala Sekaran, C.
Powiązania:
https://bibliotekanauki.pl/articles/10767.pdf
Data publikacji:
2014
Wydawca:
Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
Tematy:
opioid
tramadol
horseradish peroxidase
enzymatic method
Opis:
Three novel and sensitive enzymatic methods have been developed for the quantification of tramadol in pharmaceutical dosage forms. The proposed methods are based on the reaction of tramadol with 3-methylbenzothiazoline-2-one hydrazone (method A), aniline (method B) and aminoantipyrine in the presence of hydrogen peroxide and horseradish peroxidase to give colored complexes. The colored complexes obtained with 3-methylbenzothiazoline-2-one hydrazone, aniline and aminoantipyrine exhibit absorption maxima at 480 nm, 550 nm and 530 nm, respectively. Regression analysis of Beer’s plots showed good correlation for tramadol in the concentration range (μg/mL) 2-12 for the methods A & B and 4-24 for the method C. The experimental parameters were studied and optimized. The precision and accuracy of the methods were satisfactory. The proposed methods were successfully applied for the quantification of tramadol in 3 brands of commercially available tablet dosage forms. The results were compared satisfactorily with the official method.
Źródło:
International Letters of Natural Sciences; 2014, 11, 1
2300-9675
Pojawia się w:
International Letters of Natural Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The properties of peroxisomal ascorbate peroxidase from sugar beet (Beta vulgaris ) and expressed in E. coli
Autorzy:
Kurnik, K.
Skorupa-Klaput, M.
Tyburski, J.
Powiązania:
https://bibliotekanauki.pl/articles/80526.pdf
Data publikacji:
2013
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
conference
ascorbate peroxidase
sugar-beet
Beta vulgaris
Escherichia coli
expression
peroxidase activity
protein extract
BL21 cell
Źródło:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2013, 94, 3
0860-7796
Pojawia się w:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Bundle sheath-specific expression of the ascorbate peroxidase 2 affects photosynthesis in whole plant
Autorzy:
Gorecka, M.
Alvarez Fernandez, R.
Bialasek, M.
Mulleineaux, P.
Karpinski, S.
Powiązania:
https://bibliotekanauki.pl/articles/951327.pdf
Data publikacji:
2013
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
conference
sheath-specific expression
ascorbate peroxidase
hydrogen peroxide
photosynthesis
cytosolic peroxidase
fluorescence
electron transport chain
gene encoding
Źródło:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2013, 94, 2
0860-7796
Pojawia się w:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Deuterium isotope effects in oxidation of dopamine and norepinephrine catalyzed by horseradish peroxidase
Autorzy:
Byszewska, W.
Pająk, M.
Kańska, M.
Powiązania:
https://bibliotekanauki.pl/articles/146852.pdf
Data publikacji:
2013
Wydawca:
Instytut Chemii i Techniki Jądrowej
Tematy:
deuterium
dopamine (DA)
norepinephrine
isotope effects
oxidation
peroxidase
Opis:
Deuterium kinetic isotope effects (KIE’s) in the reaction of oxidation of dopamine (DA) catalyzed by the enzyme horseradish peroxidase (HRP, EC 1.11.1.7) was determined using a non-competitive spectrophotometric method. For kinetic studies, the deuterium ring labelled isotopomer [2’,5’,6’-2H3]-dopamine was synthesized using acid catalyzed isotopic exchange between authentic dopamine and heavy water. Deuterium solvent isotope effects (SIE’s) for dopamine and norepinephrine were determined separately for the enzymatic oxidation carried out in the presence or absence of hydrogen peroxide. Some mechanistic details of enzymatic oxidation of dopamine and norepinephrine to corresponding catecholchromes catalyzed by HRP were discussed.
Źródło:
Nukleonika; 2013, 58, 4; 475-479
0029-5922
1508-5791
Pojawia się w:
Nukleonika
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Effect of temperature on guaiacol peroxidase of Pyrus communis
Autorzy:
Saeidian, S.
Ghasemifar, E.
Powiązania:
https://bibliotekanauki.pl/articles/10793.pdf
Data publikacji:
2013
Wydawca:
Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
Tematy:
temperature effect
guaiacol
Peroxidase
Pyrus communis
enzyme
plant growth
plant development
cell wall
Opis:
Peroxidase (EC 1.11.1.7; donor: hydrogen-peroxide oxidoreductase, POD) is one of the key enzymes controlling plant growth, differentiation and development. The enzyme participates in construction, rigidification and eventual lignification of cell walls, biosynthesis of ethylene from 1-aminocyclopropane-1-carboxylic acid and H2O2, regulation of auxin level through auxin catabolism, protection of tissue from damage and infection by pathogenic microorganisms, the oxidation of indoleacetic acid. For peroxidase activity in wild pears extract one pH optimum was observed at 6.5 that probably belong to atleast one isoenzyme. Activity of peroxidase in presence of guaiacol and H2O2 was optimum after incubation at 40 °C. Maximum activity of peroxidase is 300 % .Activity increased to 240 %, 300 %, 70 % and 10 % after 60 minute incubation at 30, 40, 45 and 60 °C for peroxidase. Incubation at high temperature (70 °C) was accompanied with decrease of activity to 10 % peroxidase activity.
Źródło:
International Letters of Natural Sciences; 2013, 05
2300-9675
Pojawia się w:
International Letters of Natural Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Cadmium-induced changes in antioxidant enzymes in suspension culture of soybean cells.
Autorzy:
Sobkowiak, Robert
Rymer, Katarzyna
Rucińska, Renata
Deckert, Joanna
Powiązania:
https://bibliotekanauki.pl/articles/1043349.pdf
Data publikacji:
2004
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
ascorbate peroxidase
superoxide dismutase
peroxidase
soybean cell suspension culture
catalase
cadmium
Opis:
Cadmium (Cd), similarly to other heavy metals, inhibits plant growth. We have recently showed that Cd2+ either stimulates (1-4 μM) or inhibits (ł 6 μM) growth of soybean (Glycine max L.) cells in suspension culture (Sobkowiak & Deckert, 2003, Plant Physiol Biochem. 41: 767-72). Here, soybean cell suspension cultures were treated with various concentrations of Cd2+ (1-10 μM) and the following enzymes were analyzed by native electrophoresis: superoxide dismutase (SOD), catalase (CAT), peroxidase (POX) and ascorbate peroxidase (APOX). We found a significant correlation between the cadmium-induced changes of soybean cell culture growth and the isoenzyme pattern of the antioxidant enzymes. The results suggest that inhibition of growth and modification of antioxidant defense reactions appear in soybean cells when Cd2+ concentration in culture medium increases only slightly, from 4 to 6 μM.
Źródło:
Acta Biochimica Polonica; 2004, 51, 1; 219-222
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Effect of nanosilver on physiological performance of pelargonium plants exposed to dark storage
Autorzy:
Hatami, M.
Ghorbanpour, M.
Powiązania:
https://bibliotekanauki.pl/articles/1826.pdf
Data publikacji:
2013
Wydawca:
Instytut Ogrodnictwa
Tematy:
Pelargonium
Pelargonium x hortorum
nanosilver
leaf senescence
storage
antioxidative enzyme
lipid peroxidation
ascorbate peroxidase
guaiacol peroxidase
Źródło:
Journal of Horticultural Research; 2013, 21, 1
2300-5009
Pojawia się w:
Journal of Horticultural Research
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Blood leukocytes and eosinophil peroxidase are not related to faecal egg counts in Polish Wrzosowka hoggets naturally infected with gastrointestinal nematodes
Autorzy:
Moskwa, B.
Doligalska, M.
Cabaj, W.
Powiązania:
https://bibliotekanauki.pl/articles/839118.pdf
Data publikacji:
1998
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
parasite
gastrointestinal nematode
blood
nematode
leucocyte
eosinophil peroxidase
Źródło:
Annals of Parasitology; 1998, 44, 3
0043-5163
Pojawia się w:
Annals of Parasitology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Effect of copper(II) the activity of glutathione peroxidase in patients with head and neck cancer
Autorzy:
Malinowska, Katarzyna
Morawiec-Sztandera, Alina
Majsterek, Ireneusz
Kaczmarczyk, Dariusz
Powiązania:
https://bibliotekanauki.pl/articles/1398625.pdf
Data publikacji:
2016
Wydawca:
Index Copernicus International
Tematy:
pyrazole complex
glutathione peroxidase
head and neck cancer
Opis:
Introduction: Head and neck squamous cell carcinoma (HNSCC) accounts for about 6% of all malignant cancers. In the epidemiology of oral cavity neoplasm, important risk factors include: tobacco smoking, alcohol abuse, bad oral hygiene, papilloma virus infection, riboflavin and iron deficiency. Objective: The objective of the investigation was a synthesis of Cu(II) complex and the evaluation of antioxidative enzymatic barrier in red blood cells of patients with head and neck tumor as well as in the control group. Materials and methods: For the investigation conduction, a consent of Bioethics Committee number RNN/142/09/KB was obtained. Blood for the examination was obtained from the patients of the Dapartment of Head and Neck Neoplasms Surgery Medical University of Łódź. The experiment was conducted on the group of 40 patients with HNSCC and 40 healthy people, using spectrophotometric method, glutathione peroxidase was marked. Results: The investigation was conducted on the hemolysate obtained from the patients that were divided into two groups – a study group (1 and 2), which consisted of patients diagnosed with head and neck cancer and a control group (1 and 2) – healthy people. A significant statistical result for GPX occurred in control-1 and study-1 group with complex compound Cu(II) (p<0,001). Conclusions: Presented research prove, that complex compound Cis-dichlorobis(N1-hydroxymethyl-3methylpyrazole-κN2)copper (II) has an impact on the activity of the antioxidative GPX enzyme.
Źródło:
Polish Journal of Otolaryngology; 2016, 70, 6; 20-25
0030-6657
2300-8423
Pojawia się w:
Polish Journal of Otolaryngology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Grain discoloration in different genotypes of durum wheat (Triticum durum L.) in Argentina: associated mycobiota and peroxidase activity
Autorzy:
Cipollone, M.J.
Moya, P.
Martinez, I.
Saparrat, M.
Sisterna, M.
Powiązania:
https://bibliotekanauki.pl/articles/2084746.pdf
Data publikacji:
2020
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
durum wheat
grain discoloration
mycobiota
peroxidase activity
Opis:
Wheat grain discoloration, a worldwide disease that lowers grain quality and decreases grain yield, does not have a single etiology. It has been proposed that it is a consequence of an abiotic mechanism, a response to environmental conditions or enzymatic activity. It has also been suggest that it is a biotic mechanism, a fungal infection principally by Alternaria spp. and Bipolaris sorokiniana. The present work was carried out to analyze the possible etiology of this disease in nine durum wheat genotypes from two localities of southern Buenos Aires province (Argentina) on two sowing dates. Incidence (percentage of grain discoloration) was recorded and mycobiota associated with this pathology was registered following ISTA rules. Peroxidase activity in an extract obtained from grains belonging to genotypes of the locality that showed the highest incidence was measured. The incidence among genotypes, localities and sowing dates varied, although the genotypes with the higher and lower values of incidence were the same for all the variables tested. The fungus Alternaria spp. was isolated the most frequently followed by Fusarium spp., while Bipolaris sorokiniana was found the least frequently. Peroxidase activity showed that all the treatments had similar levels of enzymatic activity, but there was no clear differentiation between controls either between genotypes with the lowest or the highest incidence values. This suggests that peroxidase activity did not have a clear relationship with grain discoloration. In this research, it is presumed that fungal infection is the main cause of this disease.
Źródło:
Journal of Plant Protection Research; 2020, 60, 1; 14-20
1427-4345
Pojawia się w:
Journal of Plant Protection Research
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Red blood cell and plasma glutathione peroxidase activities and selenium concentration in patients with chronic kidney disease: A review
Autorzy:
Zachara, Bronisław
Gromadzińska, Jolanta
Wąsowicz, Wojciech
Zbróg, Zbigniew
Powiązania:
https://bibliotekanauki.pl/articles/1041155.pdf
Data publikacji:
2006
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
hemodialysis
selenium
kidney transplantation
glutathione peroxidase
chronic kidney disease
antioxidants
plasma
Opis:
The metabolism of oxygen in aerobic organisms leads to generation of reactive oxygen species (ROS). These entities are able to oxidize almost all classes of macromolecules, including proteins, lipids and nucleic acids. The physiological level of ROS is usually regulated by antioxidant defense mechanisms. There are at least three groups of antioxidant enzymes: superoxide dismutases, catalases and glutathione peroxidases (GSH-Pxs) which neutralize ROS. The trace elements (copper, zinc and selenium) bound to the active sites of the above listed enzymes play an important role in the antioxidant defense system. In mammals, a major function of selenium (Se) and Se-dependent GSH-Pxs is to protect cells from oxidative stress. Selenium concentrations and GSH-Px activities are altered in blood components of chronic kidney disease (CKD) patients. The Se level is frequently lower than in healthy subjects and the concentration very often decreases gradually with advancing stage of the disease. Studies on red cell GSH-Px activity in CKD patients reported its values significantly lower, significantly higher and lower or higher, but not significantly as compared with healthy subjects. On the other hand, all authors who studied plasma GSH-Px activity have shown significantly lower values than in healthy subjects. The degree of the reduction decreases gradually with the progression of the disease. High inverse correlations were seen between plasma GSH-Px activity and creatinine level. A gradual decrease in plasma GSH-Px activity in CKD patients is due to the fact that this enzyme is synthesized predominantly in the kidney and thus the impairment of this organ is the cause of the enzyme's lower activity. Se supplementation to CKD patients has a slightly positive effect in the incipient stage of the disease, but usually no effect was observed in end-stage CKD. Presently, kidney transplantation is the only treatment that may restore plasma Se level and GSH-Px activity in patients suffering from end-stage CKD. A few studies have shown that in kidney recipients, plasma Se concentration and GSH-Px activity are restored to normal values within a period of 2 weeks to 3 months following surgery and thus it can be acknowledged that Se supplementation to those patients has a positive effect on plasma GSH-Px activity.
Źródło:
Acta Biochimica Polonica; 2006, 53, 4; 663-677
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Differential response of antioxidant enzymes to cadmium stress in tolerant and sensitive cell line of cucumber (Cucumis sativus L.)
Autorzy:
Gzyl, Jarosław
Rymer, Katarzyna
Gwóźdź, Edward
Powiązania:
https://bibliotekanauki.pl/articles/1040497.pdf
Data publikacji:
2009
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
cell suspension
cadmium
tolerance
superoxide dismutase
catalase
ascorbate peroxidase
guaiacol peroxidase
Opis:
Previously, a stable cell suspension of cucumber tolerant to 100 µM CdCl2 was obtained (Gzyl & Gwóźdź, 2005, Plant Cell Tissue Organ Cult 80: 59-67). In this study, the relationship between the activity of antioxidant enzymes and cadmium tolerance of cucumber cells was analyzed. A cadmium-sensitive and the cadmium-tolerant cell lines were exposed to 100 µM and 200 µM CdCl2 and the activities of superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APOX) and guaiacol peroxidase (POX) were determined. In the sensitive cell line, a decrease of total activity of SOD and POX was observed, whereas the activity of CAT and APOX significantly increased in metal-supplemented medium. By contrast, in the tolerant cells, the total activity of antioxidant enzymes decreased (SOD, CAT) or was maintained at approximately the same level (APOX, POX). Moreover, a different pattern of isoenzyme activity was observed in the tolerant and sensitive cells. These results suggest that an enhanced activity of antioxidant enzymes is not directly involved in the increased tolerance to cadmium of the selected cucumber cell line.
Źródło:
Acta Biochimica Polonica; 2009, 56, 4; 723-727
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Porównanie oddziaływania selenu na aktywność peroksydazową gleby skażonej olejem napędowym lub przepracowanym olejem silnikowym
Comparison of selenium effect on peroxidase activities in soil contaminated with diesel oil and spent engine oil
Autorzy:
Strek, M.
Telesinski, A.
Powiązania:
https://bibliotekanauki.pl/articles/36028.pdf
Data publikacji:
2016
Wydawca:
Polska Akademia Nauk. Instytut Agrofizyki PAN
Tematy:
gleby
selen
zanieczyszczenia gleb
olej napedowy
olej silnikowy
olej silnikowy przepracowany
peroksydaza
aktywnosc peroksydazowa
soil
selenium
soil contaminant
diesel fuel
engine oil
peroxidase
peroxidase activity
Opis:
W pracy przedstawiono wyniki oddziaływania oleju napędowego, przepracowanego oleju silnikowego oraz selenu (IV i VI), na aktywność peroksydazową gleby. Doświadczenie przeprowadzono w warunkach laboratoryjnych na piasku gliniastym o zawartości węgla organicznego 8,7 g·kgˉ¹. Do próbek gleby wprowadzono w różnych kombinacjach kwas selenowy (IV) lub kwas selenowy (VI) (ilość dodanego Se wynosiła 0,05 mmol·kgˉ¹) oraz przepracowany olej silnikowy lub olej napędowy w ilościach 2, 10 i 50 g·kgˉ¹. Wszystkie próbki doprowadzono do 60% maksymalnej pojemności wodnej i przechowywano w szklanych pojemnikach typu twist, w stałej temperaturze 20°C. Aktywność peroksydaz oznaczono spektrofotometrycznie w 1., 7., 14., 28., 56. i 112. dniu doświadczenia. Skażenie gleby olejem napędowym oraz przepracowanym olejem silnikowym spowodowało stymulację aktywności peroksydaz. Wprowadzenie selenu do gleby nieskażonej substancjami ropopochodnymi wywołało zmiany aktywności peroksydaz. Jedynie dodatek selenu VI do gleby zawierającej olej napędowy w dawce 2 g·kgˉ¹ spowodował istotny wzrost średniej aktywności peroksydaz glebowych.
This paper describes the impact of diesel oil, spent engine oil and selenium (IV and VI) on peroxidase activity in soil. Experiment was carried out in laboratory conditions on loamy sand with organic carbon content of 8.7 g·kgˉ¹. Different combinations of selenic (IV) acid or selenic (VI) acid (the Se amount was 0.05 mmol·kgˉ¹) and diesel oil or spent engine oil at dosage of 2, 10 i 50 g·kgˉ¹ were added to soil samples. All samples were adjusted to 60% of the maximum water holding capacity and stored in glass twist containers at a temperature of 20°C. Activity of peroxidases was determined spectrophotometrically on days 1, 7, 14, 28, 56 and 112. Soil contamination with diesel oil and spent engine oil increased the activity of peroxidases. Application of selenium to soil uncontaminated with petroleum hydrocarbons caused significant changes of peroxidase activities in the initial period of experiment. Only in soil containing diesel oil at the dosage of 2 g·kgˉ¹ significant increase in activity of peroxidase was observed after treatment with selenium VI.
Źródło:
Acta Agrophysica; 2016, 23, 3
1234-4125
Pojawia się w:
Acta Agrophysica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Właściwości fizykochemiczne produktów enzymatycznego utleniania skrobi
Physicochemical properties of products of enzymatic oxidation of starch
Autorzy:
Roczkowska, M.
Siemion, P.
Kaczmarek, A.
Powiązania:
https://bibliotekanauki.pl/articles/410267.pdf
Data publikacji:
2013
Wydawca:
Uniwersytet Humanistyczno-Przyrodniczy im. Jana Długosza w Częstochowie. Wydawnictwo Uczelniane
Tematy:
skrobia
skrobia utleniona
utlenianie enzymatyczne
peroksydaza ligninowa
LiP
peroksydaza manganozależna
MnP
starch
oxidized starch
enzymatic oxidation
lignin peroxidase
manganese peroxidase
Opis:
Reakcje enzymatyczne coraz częściej uzupełniają i zastępują wszelkie reakcje chemiczne. Są wysoce selektywne i precyzyjne, gdyż enzymy działają na konkretne grupy czy wiązania chemiczne. Przykładem enzymów wykorzystywanych w procesach utleniania są lakazy oraz peroksydazy – ligninowa (LiP) i manganozależna (MnP). Niniejsza praca została poświęcona badaniom właściwości fizykochemicznych skrobi utlenionych enzymatycznie przy użyciu peroksydazy ligninowej (LiP) oraz manganozależnej (MnP). Badaniom zostały poddane utlenione skrobie kukurydziane oraz ziemniaczane. Badania wykazały, że we wszystkich analizowanych próbkach doszło do utlenienia enzymatycznego. Produkty modyfikacji odznaczały się odmiennymi właściwościami fizykochemicznymi w stosunku do próbek natywnych skrobi tego samego gatunku. Zmianie uległa zdolność pochłaniania wody, rozpuszczalność w wodzie i trwałość w wysokich temperaturach.
The enzymatic reactions increasingly replace and complement any chemical reactions. They are highly selective and precise, because the enzymes act on specific groups or chemical bonds. The examples of enzymes applied in the oxidation processes are laccases, lignin (LiP) and manganese (MnP) peroxidases. This work has been devoted to the studies on physicochemical properties of the enzymatically oxidized starches using lignin (LiP) and manganese (MnP) peroxidases. The studies were performed on oxidized corn and potato starches. The studies have shown that the enzymatic oxidation had occurred in all of the analyzed samples. The products of modification showed different physicochemical properties in comparison to native starch samples of the same species. The water absorption capacity, water solubility and stability at high temperatures have been altered.
Źródło:
Chemistry, Environment, Biotechnology; 2013, 16; 85-96
2083-7097
Pojawia się w:
Chemistry, Environment, Biotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Peroxidase activity and soluble carbohydrates content in pea roots in response to soil contamination with oxytetracycline
Autorzy:
Dobiesz, M.
Ziolkowska, A.
Piotrowicz-Cieslak, A.I.
Powiązania:
https://bibliotekanauki.pl/articles/80780.pdf
Data publikacji:
2013
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
conference
peroxidase activity
carbohydrate content
pea
soil contamination
oxytetracycline
Streptomyces rimosus
bacteriostatic activity
antioxidative enzyme
Źródło:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2013, 94, 3
0860-7796
Pojawia się w:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Can changes in starch content and peroxidase activity be used as rooting phase markers for rhododendron leaf bud cuttings?
Autorzy:
Megre, D.
Dokane, K.
Kondratovics, U.
Powiązania:
https://bibliotekanauki.pl/articles/19397.pdf
Data publikacji:
2011
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
content change
starch content
peroxidase activity
rooting phase marker
rhododendron
leaf bud cutting
propagation
rooting
Opis:
We examined whether peroxidase activity in cutting bases and leaves and starch content in cutting bases can be used as rooting phase markers in the elepidote rhododendron cv. 'Babites Baltais' (Rhododendron L.). Changes in peroxidase activity in cutting leaves and bases, as well as starch content in cutting bases, were determined in relation to anatomical stages of rhizogenesis in leaf bud cuttings treated with 1% indole-3-butyric acid (IBA+) or without IBA (IBA–). The pattern of change of peroxidase activity was similar in cutting bases and leaves of IBAleaf bud cuttings. Three phases of adventitious root formation were identified: induction, initiation and expression. During the induction phase peroxidase activity decreased, but no anatomical changes were observed in the cuttings. Peroxidase activity increased in the initiation phase when adventitious root initials were formed. Peroxidase activity decreased during the expression phase when adventitious root primordia developed. The starch content of IBA– leaf bud cuttings decreased during the first few days and then gradually rose to maximum, followed by a sharp reduction and another increase at the end of the experiment. The changes of starch content did not coincide with rooting phases as peroxidase activity did, and cannot be used as a rooting phase marker in rhododendrons. Adventitious root formation did not occur in IBA+ leaf bud cuttings, so distinct rooting phases could not be observed. There was a significant correlation between peroxidase activity in cutting bases and leaves of IBA- leaf bud cuttings. Peroxidase activity in leaves of rhododendron leaf bud cuttings are potentially useful as a marker for rooting phases, but that requires further anatomical and physiological study of rooting in leaf bud cuttings.
Źródło:
Acta Biologica Cracoviensia. Series Botanica; 2011, 53, 1
0001-5296
Pojawia się w:
Acta Biologica Cracoviensia. Series Botanica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The effect of methylprednisolone on the dynamics of oxygen uptake and peroxidase and superoxide dismutase activities in skeletal muscles of mice infected with Trichinella spiralis larvae
Autorzy:
Wandurska-Nowak, E.
Boczon, K.
Derda, M.
Powiązania:
https://bibliotekanauki.pl/articles/838559.pdf
Data publikacji:
1998
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
parasite
superoxide dismutase
mouse
mice
larva
peroxidase
Trichinella spiralis
skeletal muscle
Źródło:
Annals of Parasitology; 1998, 44, 3
0043-5163
Pojawia się w:
Annals of Parasitology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Superoxide dismutase and glutathione peroxidase activity in pregnancy complicated by diabetes
Autorzy:
Wdowiak, A.
Brzozowski, I.
Bojar, I.
Powiązania:
https://bibliotekanauki.pl/articles/50976.pdf
Data publikacji:
2015
Wydawca:
Instytut Medycyny Wsi
Źródło:
Annals of Agricultural and Environmental Medicine; 2015, 22, 2
1232-1966
Pojawia się w:
Annals of Agricultural and Environmental Medicine
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Selenium supplementation to chronic kidney disease patients on hemodialysis does not induce the synthesis of plasma glutathione peroxidase
Autorzy:
Zachara, Bronislaw
Gromadzinska, Jolanta
Zbrog, Zbigniew
Swiech, Rafal
Wasowicz, Wojciech
Twardowska, Ewa
Jablonska, Ewa
Sobala, Wojciech
Powiązania:
https://bibliotekanauki.pl/articles/1040656.pdf
Data publikacji:
2009
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
hemodialysis
selenium supplementation
glutathione peroxidase
chronic kidney disease
plasma
Opis:
Background: Numerous authors have shown that selenium (Se) concentration and glutathione peroxidase (GSH-Px) activity in plasma of chronic kidney disease (CKD) patients are lower than in healthy subjects, but there are only few publications on the level of GSH-Px protein in those patients and no reports on the effect of Se supplementation to HD patients on the level of this enzyme. Subjects and Methods: Se concentration and GSH-Px protein level in plasma were measured in a group of 30 CKD patients on hemodialysis (HD) supplemented with 200 µg Se/day for 3 months, and 28 patients on HD administered with placebo. Se concentration was measured by graphite furnace atomic absorption spectrometry and plasma GSH-Px protein level by the sandwich ELISA method using polyclonal antibody specific for human plasma GSH-Px. Results: Se concentration in patients on placebo did not change throughout the 3-month study period, but increased significantly in Se supplemented group. Se supplementation to CKD patients on HD had no effect on the level of GSH-Px protein. Conclusions: The lack of GSH-Px protein in CKD patients on HD is not linked to Se deficiency since the level of this element increased after Se supplementation while enzyme protein level did not change. The damaged kidney of HD patients is unable to synthesize GSH-Px, even after induction with selenium.
Źródło:
Acta Biochimica Polonica; 2009, 56, 1; 183-187
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Ligninolityczne właściwości izolatów Heterobasidion parviporum w hodowli na drewnie świerka
Ligninolytic activity of Heterobasidion parviporum isolates in cultivation on Norway spruce wood
Autorzy:
Żółciak, A.
Bohacz, J.
Powiązania:
https://bibliotekanauki.pl/articles/989467.pdf
Data publikacji:
2016
Wydawca:
Polskie Towarzystwo Leśne
Tematy:
lesnictwo
fitopatologia lesna
grzyby
Heterobasidion parviporum
izolaty grzybowe
aktywnosc lignolityczna
enzymy lignolityczne
lakaza
peroksydaza manganozalezna
peroksydaza ligninowa
peroksydaza "versatile"
rozklad drewna
drewno bielu
drewno twardzieli
drewno swierkowe
biodegradacja
laccase
manganese peroxidase
lignin peroxidase
versatile peroxidase
hydroxy−
methoxyphenols
sapwood
heartwood
norway spruce wood
Opis:
The aim of this preliminary study was to assess the activity of laccase, diverse peroxidases as well as the level of micromolecular compounds in Heterobasidion parviporum isolates grown on pieces of Norway spruce wood (sapwood and heartwood) during 50 days of incubation under the laboratory conditions. H. parviporum isolates secreted extracellular enzymes: laccase, manganese peroxidase (MnP), lignin peroxidase (LiP) and versatile peroxidase (VP). Hydroxy− and methoxyphenols were also released during this process. The above−mentioned enzymes showed low activity in mycelium grown on both sapwood and heartwood. The activity of laccase ranged from 0 to 0.513 mU/mg protein on sapwood, and from 0 to 0.106 mU/mg protein – on heartwood. MnP activity of H. parviporum isolates ranged from 0.024 to 0.667 mU/mg protein on sapwood, and from 0.038 to 1.585 mU/mg protein – on heartwood. LiP activity was small and ranged from 0 to 1.281 mU/mg protein on sapwood, and from 0.013 to 0.166 mU/mg protein – on heartwood. Activity of VP oxidizing manganese ions was low. It ranged from 0 to 3.063 mU/mg protein on sapwood, and from 0.059 to 3.054 mU/mg protein – on heartwood. The activity of VP oxidizing guaiacol ranged from 0.006 to 1.490 mU/mg protein on sapwood and from 0.038 to 1.147 mU/mg protein – on heartwood. The hydroxyphenols produced by H. parviporum isolates ranged from 15.037 to 110.149 mg of protocatechuic acid/ml on sapwood, and from 11.236 to 27.220 mg of protocatechuic acid/ml – on heartwood. Methoxyphenols produced by H. parviporum isolates ranged from 3.393 to 24.253 mg of vanillic acid/ml on sapwood, and from 4.955 to 12.005 mg of vanillic acid/ml – on heartwood.
Źródło:
Sylwan; 2016, 160, 12; 1027-1035
0039-7660
Pojawia się w:
Sylwan
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Identification of Heterobasidion spp.in Poland by RFLP analysis of laccase and manganese dependent peroxidase
Autorzy:
Kraj, W.
Kowalski, T.
Powiązania:
https://bibliotekanauki.pl/articles/41587.pdf
Data publikacji:
2010
Wydawca:
Polska Akademia Nauk. Instytut Dendrologii PAN
Opis:
Laccase and manganese dependent peroxidase (MnP) genes in H. annosum s.l. were studied by PCR-RFLP. The peroxidase genes MNP1a and MNP2 showed Heterobasidion species specific length differences. Among eighteen monomorphic markers which were foundfor investigatedgenes, three were characteristic for H. abietinum andfive for H. annosum s.s. The remaining specific markers were characteristic for H. parviporum and H. abietinum (nine markers) or for H. parviporum and H. annosum s.s. (one marker). No specific marker for H. parviporum was detected. On the basis of monomorphic markers, intersterility groups of eighty one strains isolatedfrom eleven forest tree or bush species in south Polandwere identified. Fifty-three belongedto H. annosum s.s., twenty-five to H. parviporum, andthree to H. abietinum. Strains belonging to H. annosum s.s. were isolatedfrom Pinus nigra, P. strobus, P. sylvestris, Larix decidua, Picea abies, as well as from Alnus incana, Betula pendula, Padus avium, Quercus robur and Q. rubra. H. parviporum was isolatedfrom Abies alba, Picea abies, Pinus nigra, P. sylvestris, Larix decidua andfrom Alnus incana. This is the first report of H. parviporum occurrence on Alnus incana in Poland. The ascertained H. abietinum strains derived from Abies alba. Our results demonstrate a new methodof Heterobasidion species identification based on the different length of MNP1a and MNP2 peroxidase genes and RFLP markers obtained for laccase and Mn2+ dependent peroxidase genes.
Źródło:
Dendrobiology; 2010, 63
1641-1307
Pojawia się w:
Dendrobiology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Betacyanin accumulation and guaiacol peroxidase activity in Beta vulgaris L. leaves following copper stress
Autorzy:
Morales, J.M.L.
Rodriguez-Monroy, M.
Sepulveda-Jimenez, G.
Powiązania:
https://bibliotekanauki.pl/articles/57381.pdf
Data publikacji:
2012
Wydawca:
Polskie Towarzystwo Botaniczne
Tematy:
betacyanin accumulation
guaiacol peroxidase activity
Beta vulgaris
red beet
leaf
copper stress
oxidative stress
betacyanin
abiotic stress
antioxidant compound
lipid peroxidation
copper
Opis:
The effect of copper stress on betacyanin accumulation and guaiacol peroxidase (GPOD) activity in leaves of different age was evaluated in red beet (Beta vulgaris L. var. Crosby Egyptian) plants. In hydroponic culture, plants were treated with 0.3 μM (control), 50 μM, 100 μM, and 250 μM of CuSO4 for 6 days. Copper was taken up and accumulated in old roots but was not translocated to leaves. However in young leaves, the increase of lipid peroxidation and reduction of growth were evident from day 3 of copper exposure; whereas in old leaves, the lipid peroxidation and growth were the same from either copper-treated or control plants. In response to copper exposure, the betacyanin accumulation was evident in young leaves by day 3, and continued to increase until day 6. Betacyanin only were accumulated in old leaves until day 6, but the contents were from 4 to 5 times lower than those observed in young leaves at the same copper concentrations. GPOD activity increased 3.3- and 1.4-fold in young and old leaves from day 3 of copper treatment respectively, but only in the young leaves was sustained at the same level until day 6. Old roots shown betacyanin in the control plants, but the betacyanin level and growth were reduced with the copper exposure. In contrast, young roots emerged by copper effect also accumulated copper and showed the highest betacyanin content of all plant parts assayed. These results indicate that betacyanin accumulation and GPOD activity are defense responses to copper stress in actively growing organs.
Źródło:
Acta Societatis Botanicorum Poloniae; 2012, 81, 3
0001-6977
2083-9480
Pojawia się w:
Acta Societatis Botanicorum Poloniae
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Changes of antioxidant enzymes of mung bean [Vigna radiata (L.) R. Wilczek] in response to host and non-host bacterial pathogens
Autorzy:
Farahani, A.S.
Taghavi, M.
Powiązania:
https://bibliotekanauki.pl/articles/65764.pdf
Data publikacji:
2016
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
antioxidative enzyme
enzyme
mung bean
Vigna radiata
plant response
host pathogen
non-host pathogen
resistance
bacterial pathogen
ascorbate peroxidase
catalase
peroxidase
superoxide dismutase
Opis:
The natural resistance against the majority of potential pathogens that exist in most plant species is known as non-host resistance. Several reports suggest the role of antioxidant enzymes in non-host resistance. We assayed the expression or activity of four scavenging enzymes during non-host pathogen-plant interaction (Xanthomonas hortorum pv. pelargonii/mung bean) and host pathogen-plant interaction (Xanthomonas axonopodis pv. phaseoli/mung bean). The expression of superoxide dismutase (SOD) and ascorbate peroxidase (APX) and the enzyme activity of catalase (CAT) and peroxidase (POX) were investigated. The activities of CAT and POX were higher during non-host pathogen invasion vs. host pathogen attack. The expression of SOD and APX were also different between compatible and incompatible interactions. The expression of SOD and APX were higher in the incompatible compared to the compatible interaction. Additionally, induction of the antioxidant enzymes in response to non-host pathogen was earlier than induction in response to host pathogen. Such information is important for plant breeders, and useful when looking for alternative control strategies as well.
Źródło:
Journal of Plant Protection Research; 2016, 56, 1
1427-4345
Pojawia się w:
Journal of Plant Protection Research
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Effect of priming of seeds of Medicago sativa "Bami" with gibberellic acid on germination, seedlings growth and antioxidant enzymes activity under salinity stress
Autorzy:
Younesi, O.
Moradi, A.
Powiązania:
https://bibliotekanauki.pl/articles/1828.pdf
Data publikacji:
2014
Wydawca:
Instytut Ogrodnictwa
Tematy:
salinity
seed priming
Medicago sativa
gibberellic acid
germination
seedling growth
growth
antioxidative enzyme
alfalfa
guaiacol peroxidase
catalase
ascorbate peroxidase
glutathione reductase
Źródło:
Journal of Horticultural Research; 2014, 22, 2
2300-5009
Pojawia się w:
Journal of Horticultural Research
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Characterization and analyses of hydrophobic clusters, acetylation and myristoylation sites in plant glutathione peroxidase sequences
Autorzy:
Ganguli, S.
Polley, A.
Datta, A.
Powiązania:
https://bibliotekanauki.pl/articles/10847.pdf
Data publikacji:
2015
Wydawca:
Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
Opis:
Incomplete reduction of oxygen molecules is the primary source for the formation of reactive oxygen species (ROS) during cytosolic metabolism or mitochondrial respiration. These phenomenons may be as a result of biotic or abiotic stress. Exposure to exogenous stimuli such as radiation might be an alternative pathway of ROS production. Thus plants require counter defense strategies to combat the increase of this toxic molecular build up in its cell cytoplasm. As a result they have devised an army of free radical scavenging enzymes which enable them to dissipate the oxidative stress imposed by the accumulation of these toxic moieties. Glutathione Peroxidase forms an important part of this arms race along with several catalases and organelle specific enzymes such as superoxide dismutase. Plant glutathione peroxidases(GPXs) have been studied exclusively for their evolutionary lineages since they represent a hybrid class of molecules in context of the presence and absence of selenocysteine at their catalytic centres, the former situation predominant in non vascular plant groups while the later a predominant feature of vascular plants. This analysis focuses on three important aspects of protein structure analyses – hydrophobic cluster analyses for identification of homologues, and acetylation and myristoylation sites which provide us with information regarding the post translational modifications of a particular protein group. Specific patterns of clusters along with acetylation and myristoylation site frequencies were obtained which indicate that GPXs of non vascular plant members possess less chances of getting myristoylated while acetylation was predominant in most land plant lineages but absent in aquatic members.
Źródło:
International Letters of Natural Sciences; 2015, 01
2300-9675
Pojawia się w:
International Letters of Natural Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The effect of seedling chilling on glutathione content, catalase and peroxidase activity in Brassica oleracea L. var. italica
Autorzy:
Wojciechowska, R.
Hanus-Fajerska, E.J.
Kolton, A.
Kaminska, I.
Grabowska, A.
Kunicki, E.
Powiązania:
https://bibliotekanauki.pl/articles/57934.pdf
Data publikacji:
2013
Wydawca:
Polskie Towarzystwo Botaniczne
Tematy:
seedling
chilling stress
glutathione content
catalase activity
peroxidase activity
Brassica oleracea var.italica
broccoli
development stage
Opis:
The study was designed to determine the possible relationship between Brassica oleracea var. italica seedlings stored at 2°C in the dark for seven and fourteen days, respectively, and the level of certain antioxidant parameters in particular organs. A parallel objective of the experiment was to determine if the reaction of seedlings to low temperature might be persistent in fully developed plants until harvest time. After 14 days of chilling a significant increase in the glutathione content was observed in the seedling leaves in comparison to the non-chilled plants. During vegetation in field conditions this effect was maintained in leaves up to the stage of formation of flower buds. At harvest the highest content of glutathione was demonstrated in broccoli heads, obtained from plants, which were previously chilled in the seedling phase for two weeks. Peroxidase activity in broccoli seedlings increased each year of the three-year study due to the duration of the cooling time, whereas in the case of catalase the changes were not so distinct. At harvest time the activity of both enzymes in the leaves and flower buds fluctuated according to the particular year of study.
Źródło:
Acta Societatis Botanicorum Poloniae; 2013, 82, 3
0001-6977
2083-9480
Pojawia się w:
Acta Societatis Botanicorum Poloniae
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Activity of superoxide dismutase, catalase and glutathione peroxidase in rats exposed to chlorpyrifos and erofloxacin
Autorzy:
Barski, D.
Spodniewska, A.
Zasadowski, A.
Powiązania:
https://bibliotekanauki.pl/articles/32363.pdf
Data publikacji:
2011
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
The aim of the study was to investigate the influence of administration of chlorpyrifos and/or enrofloxacin on the activity of chosen antioxidative enzymes i.e.: superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx) in erythrocytes of rats. Chlorpyrifos was administered by stomach tube during 28 days at a dose of 3 mg/kg bw (0.02 LD50), and enrofloxacin was administered by stomach tube at a dose of 5 mg/kg bw during 3 subsequent days. It was stated that administration of enrofloxacin at applied dose did not cause any major changes in the activity of investigated antioxidative enzymes. The four-week exposure of rats to chlorpyrifos caused noticeable decrease in SOD and CAT activity in erythrocytes of rats at the beginning of the experiment (up to 24th hour) in comparison with the control group. The activity of GPx during all periods of the experiment was increased. In the group of animals in which both chlorpyrifos and enrofloxacin were applied, the profile of changes in activity of examined enzymes was similar to that one, which was observed after administration of chlorpyrifos exclusively, what may indicate lack of co-action between compounds used in the experiment.
Źródło:
Polish Journal of Veterinary Sciences; 2011, 14, 4
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Effect of 8-hydroxyquinoline citrate, sucrose and peroxidase inhibitors on vase life of Lisianthus (Eustoma grandiflorum L.) cut flowers
Autorzy:
Sharifzadeh, K.
Asil, M.H.
Roein, Z.
Sharifzadeh, M.
Powiązania:
https://bibliotekanauki.pl/articles/2024.pdf
Data publikacji:
2014
Wydawca:
Instytut Ogrodnictwa
Tematy:
Lisianthus
Eustoma grandiflorum
peroxidase
8-hydroxyquinoline citrate
sucrose
superoxide dismutase
malondialdehyde
water uptake
protein
anthocyanin content
cut flower
quality parameter
vase life
Źródło:
Journal of Horticultural Research; 2014, 22, 1
2300-5009
Pojawia się w:
Journal of Horticultural Research
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Mikrofluidyka - technologia miniaturyzacji laboratorium
Autorzy:
Laskowski, M.
Powiązania:
https://bibliotekanauki.pl/articles/274256.pdf
Data publikacji:
2011
Wydawca:
Roble
Tematy:
mikrofluidyka
technologie mikrocieczowe
mikromacierze DNA
lab-on-a-chip
droplet-on-demand
peroksydaza
microfluidics
DNA microarrays
peroxidase
Źródło:
LAB Laboratoria, Aparatura, Badania; 2011, 16, 1; 41-43
1427-5619
Pojawia się w:
LAB Laboratoria, Aparatura, Badania
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Antioxidant defence in maize cultivars triggered by the two-spotted spider mite attack and soil drought – a comparative study
Autorzy:
Czapla, A.
Grudkowska, M.
Miazek, A.
Zagdanska, B.
Kielkiewicz, M.
Powiązania:
https://bibliotekanauki.pl/articles/80869.pdf
Data publikacji:
2013
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
conference
antioxidant defense
maize cultivar
two-spotted spider mite
Tetranychus urticae
soil drought
oxidative stress
ascorbate peroxidase
catalase
glutathione reductase
peroxidase
polyphenol oxidase
Źródło:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2013, 94, 2
0860-7796
Pojawia się w:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Wpływ zmian ciśnienia otoczenia na aktywność peroksydazy glutationowej (GPX) i atalazy (CAT) we krwi nurków : badania wstępne
The effect of ambient pressure changes on the activity of glutathione peroxidase (GPX) and catalase (CAT) in the blood divers : preliminary results
Autorzy:
Włodarski, A.
Woźniak, A.
Mila-Kierzenkowska, C.
Sutkowy, P.
Powiązania:
https://bibliotekanauki.pl/articles/366531.pdf
Data publikacji:
2013
Wydawca:
Polskie Towarzystwo Medycyny i Techniki Hiperbarycznej
Tematy:
ciśnienie
nurkowanie
peroksydaza glutationowa
katalaza
pressure
diving
glutathione peroxidase
catalase
Opis:
Zmiana warunków otoczenia jest stresem dla organizmu, który może prowadzić do wzmożonej generacji reaktywnych form tlenu i licznych uszkodzeń w obrębie komórek. Istotną rolę w przeciwdziałaniu tym uszkodzeniom pełnią enzymy antyoksydacyjne. Celem niniejszej pracy było określenie wpływu zmian ciśnienia otoczenia w warunkach nurkowania na aktywność peroksydazy glutationowej oraz katalazy. W badaniu wzięło udział 11 płetwonurków w wieku od 18 do 41 lat. Badani spędzili 40 min w jeziorze, w wodzie o temperaturze 13° C, schodząc maksymalnie na głębokość około 9 m. Podczas zanurzenia wszyscy oddychali powietrzem. Krew do badań pobrano z żyły odłokciowej dwukrotnie: przed zanurzeniem w wodzie (kontrola) oraz bezpośrednio po wynurzeniu. W pracy wykazano tendencję do wzrostu aktywności katalazy oraz tendencję do obniżania się aktywności peroksydazy glutationowej, ale zmiany te nie były istotne statystycznie. Ciśnienie otoczenia wywierane przez wodę podczas nurkowania nie ma istotnego wpływu na aktywność peroksydazy glutationowej oraz katalazy w erytrocytach. Może to sugerować, że nie doszło do zwiększonej generacji nadtlenku wodoru będącego substratem reakcji katalizowanych przez oba enzymy. Brak istotnych statystycznie zmian aktywności badanych enzymów antyoksydacyjnych może być efektem zmian przystosowawczych w organizmie osób nurkujących.
The changes in ambient conditions are usually stress factor for the organism, which may lead to increased generation of reactive oxygen species and numerous damages within the cells. The important role in counteracting of free radical damages to cells is played by antioxidant enzymes. The aim of this study was to determine the impact of changes in ambient pressure during diving on activity of selected antioxidant enzymes: glutathione peroxidase and catalase . The study involved eleven divers, aged from 18 to 41 years. Subjects spent 40 minutes in the lake, in water at 13° C, going down to a depth of about 9 m. All divers breathe with the air. Blood samples were taken from basilica vein twice: before immersion in water (control) and immediately after surfacing. The study showed an increasing trend in catalase activity and trend to decrease in glutathione peroxidase activity, but the changes were not statistically significant. Ambient pressure exerted by the water during diving has no significant effect on the activity of glutathione peroxidase and catalase in erythrocytes. This may suggest, that there was no increased generation of hydrogen peroxide which is a substrate of the reaction catalyzed by two enzymes. No statistically significant differences in the activity of antioxidant enzymes could be due to adaptive changes in the body of divers.
Źródło:
Polish Hyperbaric Research; 2013, 1(42); 7-25
1734-7009
2084-0535
Pojawia się w:
Polish Hyperbaric Research
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Antioxidant status in erythrocytes of cystic fibrosis children.
Autorzy:
Laskowska-Klita, Teresa
Chełchowska, Magdalena
Powiązania:
https://bibliotekanauki.pl/articles/1044199.pdf
Data publikacji:
2001
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
superoxide dismutase
catalase
glutathione peroxidase
vitamin E
erythrocytes
cystic fibrosis
Opis:
Activities of superoxide dismutase, catalase and glutathione peroxidase in erythrocytes of cystic fibrosis children were studied in order to estimate the severity of their deficiency. Our results point to increased susceptibility of erythrocytes of cystic fibrosis subjects to oxidative injury and indicate that the antioxidant status of patients should be carefully monitored.
Źródło:
Acta Biochimica Polonica; 2001, 48, 1; 283-285
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The activities of superoxide dismutase, catalase, glutathione peroxidase and glutathione redudase in erythrocytes of rats with experimental neoplastic disease
Autorzy:
Batko, Jolanta
Warchoł, Teresa
Karoń, Halina
Powiązania:
https://bibliotekanauki.pl/articles/1045161.pdf
Data publikacji:
1996
Wydawca:
Polskie Towarzystwo Biochemiczne
Źródło:
Acta Biochimica Polonica; 1996, 43, 2; 403-405
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The participation of peroxidase in adaptation of plants to moderate water deficit
Udział peroksydaz w adaptacji roślin do warunków umiarkowanego deficytu wody
Autorzy:
Nedukha, O.M.
Powiązania:
https://bibliotekanauki.pl/articles/797468.pdf
Data publikacji:
2006
Wydawca:
Szkoła Główna Gospodarstwa Wiejskiego w Warszawie. Wydawnictwo Szkoły Głównej Gospodarstwa Wiejskiego w Warszawie
Opis:
The aim of this investigation was to study the localization and activity of peroxidase (EC. 1.11.1.7) in the leaves of Sium latifolium L. and interaction of peroxidase activity with lignin synthesis at moderate water deficit. The leaves of two ecological forms (ecotypes) in flowering phase were studied. S. latifolium air- aquatic ecotype grew in water (on the bank of Psel River, Ukraine), and terrestrial ecotype plant grew along the bank of this river under the conditions of moderate water deficit. The immunocytochemical and biochemical methods were used for the investigation. Peroxidase activity was estimated in chloroplasts of both palisade and spongy mesophyll and in cell walls of epidermis and mesophyll. The adaptation of S. latifolium plants to the moderate water deficit leads to the increase of guaiacol- and ascorbate peroxidase activities and lignin content in leaves, and also to the appearance of peroxidase in vacuoles.
Zbadano lokalizację i aktywności peroksydaz (EC. 1.11.1.7) w liściach Sium latifolium oraz określono interakcję pomiędzy aktywnością enzymu i syntezą lignin w warunkach umiarkowanego deficytu wody. Badania przeprowadzono na dwóch ekotypach S. latifolium w fazie kwitnienia roślin. Uprawę ekotypu powietrzno-wodnego prowadzono w wodzie, na brzegu rzeki Psel na Ukrainie, a ekotypu lądowego wzdłuż brzegu rzeki w warunkach umiarkowanego deficytu wody. Zasosowano immunocytochemiczne i biochemiczne metody analizy aktywności peroksydazy i zawartości lignin. Aktywność peroksydazy oznaczono w chloroplastach miękiszu palisadowego i gąbczastego oraz w ścianach komórkowych epidermy i mezofilu. Adaptacja S. latifolium do waruków umiarkowanego deficytu wody wiązała się ze wzrostem aktywności enzymów i zawartością lignin, a także pojawieniem się peroksydaz w wakuolach liści ekotypu lądowego.
Źródło:
Zeszyty Problemowe Postępów Nauk Rolniczych; 2006, 509
0084-5477
Pojawia się w:
Zeszyty Problemowe Postępów Nauk Rolniczych
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Plant peroxidases and nitrogen metabolism: interactions with nitrates and nitrites
Autorzy:
Minibayeva, F.
Galeeva, E.
Gurjanov, O.
Viktorova, L.
Powiązania:
https://bibliotekanauki.pl/articles/80640.pdf
Data publikacji:
2013
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
conference
peroxidase
plant
nitrogen metabolism
oxidoreductase
nitrate
nitrite
Źródło:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2013, 94, 2
0860-7796
Pojawia się w:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Isolation and partial purification of a glutathione peroxidase from pig blood platelets
Izolowanie i częściowe oczyszczanie peroksydazy glutationowej z płytek krwi świni
Autorzy:
Żbikowska, Halina
Wachowicz, Barbara
Powiązania:
https://bibliotekanauki.pl/articles/945016.pdf
Data publikacji:
1996
Wydawca:
Uniwersytet Łódzki. Wydawnictwo Uniwersytetu Łódzkiego
Opis:
Metodą Maddipati i Marnett (1987) wyizolowano i częściowo scharakteryzowano peroksydazę glutationową z płytek krwi świni. Proces oczyszczania przebiegał kolejno poprzez wysalanie siarczanem amonu, hydrofobową chromatografię na złożu Phenyl-Sepharose CL-4B oraz chromatografię jonowymienną. Elektroforeza w żelu poliakryloamidowym z SDS (w obecności /3-merkaptoetanolu) enzymu otrzymanego w ostatnim etapie oczyszczania ujawniła obecność podjednostki o masie cząsteczkowej 23 kDa. Wykazano, że w płytkach dominującą formą GSH-Px jest selenozależna peroksydaza glutationowa, ktora wykazuje ok. 86% całkowitej aktywności tego enzymu. Selenin sodowy powodował wzrost aktywności otrzymanego enzymu, podczas gdy jony miedzi, NEM i cisplatyna była inhibitorami peroksydazy glutationowej.
Źródło:
Acta Universitatis Lodziensis. Folia Biochimica et Biophysica; 1996, 11
0208-614X
Pojawia się w:
Acta Universitatis Lodziensis. Folia Biochimica et Biophysica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Generation of superoxide anion and induction of superoxide dismutase and peroxidase in bean leaves infected with pathogenic fungi
Autorzy:
Urbanek, Henryk
Gajewska, Ewa
Karwowska, Renata
Wielanek, Marzena
Powiązania:
https://bibliotekanauki.pl/articles/1045088.pdf
Data publikacji:
1996
Wydawca:
Polskie Towarzystwo Biochemiczne
Źródło:
Acta Biochimica Polonica; 1996, 43, 4; 679-685
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Growth regulators and guaiacol peroxidase activity during the induction phase of somatic embryogenesis in Picea species
Autorzy:
Hazubska-Przybyl, T.
Ratajczak, E.
Kalemba, E.M.
Bojarczuk, K.
Powiązania:
https://bibliotekanauki.pl/articles/41387.pdf
Data publikacji:
2013
Wydawca:
Polska Akademia Nauk. Instytut Dendrologii PAN
Opis:
Biochemical studies during the induction phase of somatic embryogenesis in Picea abies [L.] Karst. and P. omorika [Pančić] Purk. can supplement our basic knowledge of the developmental processes accompanying the formation of embryogenic tissues from explants. Such studies may also contribute to finding the markers specific to the early stages of somatic embryogenesis of spruce species and, consequently, to the optimization of the process of initiation of embryogenic tissues from different types of plant explants treated with various growth regulator combinations. In this paper the effect of certain growth regulator systems on enzymatic activity was studied. The analysis of guaiacol peroxidase activity (EC 1.11.1.7), based on the spectrophotometric method, showed that this activity was the lowest in mature zygotic embryos (explants) and significantly higher in 8-week-old embryogenic and non-embryogenic calluses treated with various combinations of growth regulators. In the newly initiated embryogenic tissue, the activity of this enzyme decreased and remained at a lower level during proliferation, irrespective of the applied growth regulator combination. The type and concentration of growth regulators used for the initiation and proliferation of embryogenic tissues had no statistically significant effect on peroxidase activity, although during the initiation often its increased level was observed in calluses treated with 2.4-D. Detection of guaiacol peroxidase activity in the induction phase of somatic embryogenesis proves its participation in this process. The subsequent change in its activity indicates that this peroxidase can be a biochemical marker of somatic embryogenesis of the tested spruce species.
Źródło:
Dendrobiology; 2013, 69
1641-1307
Pojawia się w:
Dendrobiology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Redox state modulation in rice and its implications for plant development and stress responses
Autorzy:
Caverzan, A.
Ribeiro, C.
Lazzarotto, F.
Passaia, G.
Rosa, S.
Garighan, J.
Piovesana, M.
Carvalho, F.
Albenisio, J.
Margis, R.
Margis-Pinheiro, M.
Powiązania:
https://bibliotekanauki.pl/articles/79908.pdf
Data publikacji:
2013
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
conference
reactive oxygen species
scavenging enzyme
detoxification
redox state
rice
plant development
stress response
plant cell
biochemical property
antioxidative enzyme
ascorbate peroxidase
glutathione peroxidase
Źródło:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2013, 94, 2
0860-7796
Pojawia się w:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Class III peroxidases in maize plasma membranes
Autorzy:
Moller, B.
Luthje, S.
Powiązania:
https://bibliotekanauki.pl/articles/79851.pdf
Data publikacji:
2013
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
conference
class III peroxidase
maize
plasma membrane
isoenzyme
thylakoid membrane
tonoplast membrane
oxidative stress
Źródło:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2013, 94, 2
0860-7796
Pojawia się w:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Isolation and computational characterization of glutathione peroxidase gene from an aquatic fern - Salvinia molesta
Autorzy:
Rahaman, S.
Singh, P.K.
Basu, P.
Gupta, S.
Basu, M.
Ganguli, S.
Powiązania:
https://bibliotekanauki.pl/articles/11200.pdf
Data publikacji:
2016
Wydawca:
Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
Opis:
Pteridophytes and more specifically ferns represent a large but threatened group of plants which often serve as important environmental markers for pollution. Reports regarding stress responses in ferns are rare, apart from a few studies involving the ecological distribution and molecular marker studies. This work isolates a glutathione peroxidase enzyme from an aquatic fern widely distributed in fresh and polluted water bodies adjacent to sources of environmental polluted sources. Further computational analyses were performed to study the structure of the protein encoded by the open reading frame. Results indicate the presence of a large number of binding pockets which serve as important binding sites in the interactions with the cognate ligands.
Źródło:
International Letters of Natural Sciences; 2016, 51
2300-9675
Pojawia się w:
International Letters of Natural Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Purification of peroxidase from the medicinal herb Andrographis paniculata causing fungal hyphal distortion
Oczyszczanie peroksydazy z leczniczej rośliny Andrographis paniculata, powodującej zniekształcenia strzępek grzybni
Autorzy:
Ghosh, M.
Powiązania:
https://bibliotekanauki.pl/articles/71287.pdf
Data publikacji:
2010
Wydawca:
Instytut Włókien Naturalnych i Roślin Zielarskich
Opis:
Peroxidases are ubiquitous in plants and their pattern of expression depends on tissue, development stage and environmental stimuli. They are involved in numerous physiological functions, including defense against pathogens and insect pests. The present study was conducted to isolate and purify a constitutive leaf peroxidase from the medicinal herb, Andrographis paniculata. The enzyme was purified through a series of purification steps and a 35kDa peroxidase was purified to homogeneity with pI value of 6.0. In vitro hyphal extension inhibition assay against the forest pathogen Trichosporium vesiculosum revealed significant morphological distortion of the protein treated hyphae.
Peroksydazy są wszechobecne w roślinach. Forma ich ekspresji zależy od tkanki, stadium rozwojowego i wpływu środowiska. Są one związane z licznymi funkcjami fizjologicznymi, w tym z obroną przed patogenami i szkodnikami. Celem przedstawionej pracy było wyizolowanie i oczyszczenie peroksydazy z liści rośliny leczniczej Andrographis paniculata. Enzym był oczyszczany wieloetapowo i peroksydaza 35 kDa była oczyszczona jako jednorodna przy pI=6,0. W doświadczeniach prowadzonych in vitro metodą zahamowania wzrostu grzybni Trichosporium vesiculosum (patogen leśny) wykazano istotne zniekształcenia białek badanej grzybni.
Źródło:
Herba Polonica; 2010, 56, 2
0018-0599
Pojawia się w:
Herba Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Disintegration of sludge by a two-stage treatment with hydrogen peroxide and solid-state fermentation by Aspergillus oryzae CGMCC5992
Autorzy:
Guan, G.
Zhicai, Z.
Jun, J.
Fan, F.
Keping, C.
Powiązania:
https://bibliotekanauki.pl/articles/208076.pdf
Data publikacji:
2016
Wydawca:
Politechnika Wrocławska. Oficyna Wydawnicza Politechniki Wrocławskiej
Tematy:
Aspergillus oryzae CGMCC5992
fermentation
lignin peroxidase
manganese peroxidase
solid state fermentation
wastewater sludge
wastewater treatment plants
lignina peroksydazy
mangan peroksydazy
podłoże organiczne
fermentacja w stanie stałym
osady ściekowe
oczyszczalnie ścieków
Opis:
A novel and safe method has been reported for wastewater sludge treatment, the first step of which is H2O2 pretreatment at 150 cm3kg for 80 min. In this step, the majority of organic substrate was degraded, and the shear stress diminished significantly. In the second step, Aspergillus oryzae further decomposed the organic substrate of sludge for 8 days, and the activities of manganese peroxidase and lignin peroxidase kept increasing. By the methods, the residual chemical oxygen demand (COD) in the sludge was much lower than that in the sludge treated with high doses of H2O2. These results indicate that the present study provides a feasible method to safely dispose sludge from the wastewater treatment plant.
Źródło:
Environment Protection Engineering; 2016, 42, 3; 43-54
0324-8828
Pojawia się w:
Environment Protection Engineering
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
A seleno enzyme, glutathione peroxidase from human placenta: Purification and some properties
Peroksydaza głutationowa selenozależny enzym z łożyska ludzkiego: oczyszczanie i niektóre właściwości
Autorzy:
Gromadzinska, Jolanta
Zachara, Bronisław A
Powiązania:
https://bibliotekanauki.pl/articles/944964.pdf
Data publikacji:
1992
Wydawca:
Uniwersytet Łódzki. Wydawnictwo Uniwersytetu Łódzkiego
Źródło:
Acta Universitatis Lodziensis. Folia Biochimica et Biophysica; 1992, 9
0208-614X
Pojawia się w:
Acta Universitatis Lodziensis. Folia Biochimica et Biophysica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Similarity between enzymatic and electrochemical oxidation of 2-hydroxyacridinone, the reference compound of antitumor imidazoacridinones.
Autorzy:
Mazerska, Zofia
Powiązania:
https://bibliotekanauki.pl/articles/1043630.pdf
Data publikacji:
2003
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
peroxidase-mediated oxidation
antitumor acridinones
enzymatic activation
enzymatic and electrochemical transformations
Opis:
The present work is part of a wide research project aimed to elucidate the mechanism of the metabolic activation of the antitumor imidazoacridinone agent C-1311 selected for clinical trials. The objectives of the investigations presented here were: (i) to examine the enzymatic transformation of the reference compound 2-hydroxyacridinone and (ii) to test the similarity between enzymatic and electrochemical oxidation of acridinone compounds. This similarity was searched with respect to the usefulness of the electrochemical results for further studies on the metabolic oxidation of imidazoacridinone antitumor drugs. The enzymatic oxidation of 2-hydroxyacridinone was performed with a model system containing various amounts of horseradish peroxidase and hydrogen peroxide and was followed by UV-VIS spectroscopy and by HPLC. One product of the reaction was isolated and its chemical structure was identified. It was shown that 2-hydroxyacridinone was transformed by the studied system in a manner dependent on the amount of the enzyme and on the compound/H2O2 ratio. While under mild reaction conditions the transformation ran slowly to yield only one product, p1, independently of the reaction time, higher enzyme concentration resulted in several steps of transformation. Product p1 turned out to be a dimer: 1,1-bi(2-hydroxyacridinone). A comparison of the results of the enzymatic transformations of 2-hydroxyacridinone presented here with studies on the electrochemical oxidation reported earlier allowed us to show both transformations to be similar as far as the reaction pathway and two reaction products are concerned.
Źródło:
Acta Biochimica Polonica; 2003, 50, 2; 515-525
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Lichen peroxidases have both pro- and antioxidant roles
Autorzy:
Beckett, R.
Minibayeva, F.
Liers, C.
Powiązania:
https://bibliotekanauki.pl/articles/81039.pdf
Data publikacji:
2013
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
conference
lichen
peroxidase activity
prooxidative property
antioxidative property
hydrogen peroxide
reactive oxygen species
Źródło:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2013, 94, 2
0860-7796
Pojawia się w:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Maturation of bovine oocytes under low culture temperature decreased glutathione peroxidase activity of both oocytes and blastocysts
Autorzy:
Şen, U.
Powiązania:
https://bibliotekanauki.pl/articles/2087228.pdf
Data publikacji:
2021
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
bovine
temperature
antioxidant activity
oxidative stress
oocytes
blastocysts
Źródło:
Polish Journal of Veterinary Sciences; 2021, 24, 1; 93-99
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Peroksydazy - małe enzymy o wielkim znaczeniu
Autorzy:
Pawlowska, A.
Treder, K.
Powiązania:
https://bibliotekanauki.pl/articles/835039.pdf
Data publikacji:
2014
Wydawca:
Instytut Hodowli i Aklimatyzacji Roślin
Tematy:
enzymy
peroksydaza
mechanizm dzialania
zastosowanie
metody otrzymywania
wycierka ziemniaczana
enzyme
peroxidase
action mechanism
enzyme application
potato pulp
Źródło:
Ziemniak Polski; 2014, 24, 1
1425-4263
Pojawia się w:
Ziemniak Polski
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Functional characterization of A. thaliana chloroplastic Prx II E
Autorzy:
Treffon, P.
Konig, J.
Dietz, K.
Powiązania:
https://bibliotekanauki.pl/articles/79923.pdf
Data publikacji:
2013
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
conference
reactive oxygen species
chloroplast
plant cell
peroxiredoxin
Arabidopsis thaliana
peroxidase activity
functional characteristics
Źródło:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2013, 94, 2
0860-7796
Pojawia się w:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Reakcja fasoli szparagowej na zasolenie podłoża
French bean reaction on basis salinity
Autorzy:
Herman, B.
Biczak, R.
Rychter, P.
Powiązania:
https://bibliotekanauki.pl/articles/410291.pdf
Data publikacji:
2010
Wydawca:
Uniwersytet Humanistyczno-Przyrodniczy im. Jana Długosza w Częstochowie. Wydawnictwo Uczelniane
Tematy:
zasolenie
fasola
karotenoidy
katalaza
peroksydaza
salinity
bean
chlorophyll
carotenoides
catalase
peroxidase
Opis:
W przeprowadzonym eksperymencie wazonowym określono wpływ zróżnicowanego zasolenia podłoża na aktywność katalazy i peroksydazy oraz zawartość chlorofilu całkowitego i karotenoidów w liściach fasoli szparagowej (Phaseolus vulgaris L., cv Złota Saxa). Rośliny potraktowano doglebowo roztworami chlorku sodu zawierającymi: 5, 10, 20 i 30 mM NaCl. Uzyskane wyniki wykazały, że zarówno zmiany aktywności badanych enzymów, jak i zawartości barwników asymilacyjnych w liściach roślin były w dużym stopniu zależne od poziomu zasolenia gleby. Zasolenie podłoża prowadziło do wzrostu aktywności peroksydazy w liściach fasoli, wielkość zmian była wprost proporcjonalna do poziomu zasolenia. Aktywność katalazy w liściach fasoli rosnącej na podłożu o niższych poziomach zasolenia była zbliżona do aktywności w roślinach kontrolnych, podczas gdy wyższe zasolenia wywołały spadek aktywności tego enzymu. Zasolenie podłoża wpłynęło także na obniżenie zawartości chlorofilu i karotenoidów w liściach fasoli, przy czym zmiany te były tym większe im wyższy był poziom zasolenia gleby.
The effect of various soil salinities on changes of catalase and peroxidase activity, content of total chlorophyll and carotenoides in leaves of French bean (Phaseolus vulgaris L., cv Golds Saxa) in the pot experiments was determined. The plants were watered with a solution of sodium chloride containing 5, 10, 20 and 30 mM NaCl. The results have proved that the salinity levels applied to the basis influenced not only the change in the activity of the enzymes studied but also assimilation pigments content in leaves of plants. The basis salinity caused increase the peroxidase activity in leaves of bean and was directly proportional to the level of basis salinity. Activity of the catalase in leaves of bean growing in soil containing lower levels of salinity was similar to the activity in control plants, while higher salinities caused considerable drop in activity of these enzyme. As expected excessive salinity of soil caused decrease of chlorophyll and carotenoides level in tested plants. The higher salinity level was, the more erident changes were observed.
Źródło:
Chemistry, Environment, Biotechnology; 2010, 14; 111-120
2083-7097
Pojawia się w:
Chemistry, Environment, Biotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Does the peroxidase-like activity of sodium dodecyl sulphate-modified cytochrome c increase after peroxynitrite or radiation treatment?.
Autorzy:
Gębicka, Lidia
Didik, Joanna
Powiązania:
https://bibliotekanauki.pl/articles/1041449.pdf
Data publikacji:
2005
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
cytochrome c
peroxynitrite; radiation
SDS
peroxidatic activity
Opis:
The peroxidase-like activity of cytochrome c is considerably increased by unfolding of the protein. The enhancement of the activity is due to the higher reaction rate of unfolded cytochrome c with hydrogen peroxide, which is the rate-determining step in the peroxidase cycle of cytochrome c (Gębicka, L., 2001, Res Chem Intermed 27, 717-23). In this study we checked whether combined action of two unfolding factors, SDS and peroxynitrite or radiation (hydroxyl radicals), increases the peroxidase-like activity of cytochrome c more than any single treatment alone. Peroxynitrite reacts with SDS-modified cytochrome c in the same way as with native cytochrome c, via intermediate radical products, •OH/•NO2, arising from peroxynitrite homolysis. We found that SDS-modified cytochrome c is much more sensitive to oxidative damage than the native protein. Partial unfolding of cytochrome c by SDS causes the peroxide substrate to have a better access to the heme center. On the other hand, the amino acids located in the vicinity of the active site and/or heme group become accessible for oxidizing radicals. The overall effect observed is that the peroxidase-like activity of SDS-modified cytochrome c decreases with an increase of the concentration of the oxidizing species (peroxynitrite or radiolytically generated hydroxyl radicals). The damage of SDS-modified cytochrome c caused by irradiation is much more significant than that observed after peroxynitrite treatment.
Źródło:
Acta Biochimica Polonica; 2005, 52, 2; 551-555
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Zmiany aktywności peroksydatywnej i poziomu witaminy C w procesie kiszenia kapusty
Changes in peroxidase activity and in level of vitaimin C on pickling the cabbage
Autorzy:
Chmielnicka, J.
Mlodecki, H.
Grabska, K.
Powiązania:
https://bibliotekanauki.pl/articles/872553.pdf
Data publikacji:
1967
Wydawca:
Narodowy Instytut Zdrowia Publicznego. Państwowy Zakład Higieny
Źródło:
Roczniki Państwowego Zakładu Higieny; 1967, 18, 1
0035-7715
Pojawia się w:
Roczniki Państwowego Zakładu Higieny
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Influence of selenium on oxidative stress in athletes. Review article
Autorzy:
Sieja, Krzysztof
von Mach-Szczypińska, Joanna
Kois, Natalia
Ler, Paulina
Piechanowska, Klaudia
Stolarska, Michalina
Powiązania:
https://bibliotekanauki.pl/articles/1055002.pdf
Data publikacji:
2016
Wydawca:
Uniwersytet Szczeciński. Wydawnictwo Naukowe Uniwersytetu Szczecińskiego
Tematy:
athletes
gluthatione peroxidase
malondialdehyde
physical exercise
selenium
Opis:
The aim of the study was to analyze the influence of selenium (Se) on the course of oxidative stress in trained athletes, on the basis of own former published studies and the reference of literature. In a number of references it was shown that the application of Se led to the diminishing of oxidative stress during the physical exercises in athletes. The application of Se to athletes − led to the increase of peroxidase glutathione the main selen − dependent enzyme. The fact of increased concentration of malondialdehyde (MDA) in blood serum, the main metabolite of lipid peroxidation can indirectly evidence about the intensity of physical endurance. This increase is significantly smaller in individuals taking exogenous antioxidative diet supplement containing Se. Moreover there is the evidence that oxidative muscles damage during physical effort could be diminished by compounds with antioxidative properties. Taking into consideration, the obtained results by many of authors empower to the conclusion that application of Se diminishes the degree of peroxidation of lipids in trained athletes.
Źródło:
Central European Journal of Sport Sciences and Medicine; 2016, 14, 2; 87 - 92
2300-9705
2353-2807
Pojawia się w:
Central European Journal of Sport Sciences and Medicine
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Removal of a textile dye (RBBR) from the water environment by fungi isolated from lignocellulosic composts
Autorzy:
Bohacz, Justyna
Powiązania:
https://bibliotekanauki.pl/articles/204714.pdf
Data publikacji:
2020
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
water environment
textil dye
Trichoderma sp
peroxidase activity
Remazol Brilliant Blue R.
Opis:
A representative group of hydrophilic fungi from the genus Trichoderma isolated from lignocellulose composts with varying degrees of maturity was analyzed for their ability to biodegrade a harmful anthraquinone dye, i.e. Remazol Brilliant Blue R (RBBR). In RBBR-containing post-culture liquids, there were determined the degree of RBBR decolorization, horseradish peroxidase-like, superoxide dismutase-like, and xylanase activities, and the concentrations of low-molecular phenolic compounds. The study results demonstrated that Trichoderma asperellum, T. harzianum, and T. lixii strains isolated from compost containing larger amounts of easily available lignocellulose fractions, i.e. grasses, exhibit higher RBBR decolorization efficiency ranging from 0.3 to 62% than T. citrinoviride strains isolated from compost II, which contained greater quantities of hardly degradable lignocellulose. The decolorization of remazol blue R by the investigated Trichoderma strains intensified significantly with the increase in peroxidase activity and it was correlated with a decline in the content of low-molecular phenolic compounds. The dynamics of changes in the horseradish peroxidase-like, superoxide dismutase, and xylanase activities in the aqueous post-culture liquids of the investigated fungal strains depended largely on the duration of the culture. Given their ability to adapt to water environments, e.g. wastewater, and to decolorize and detoxify the RBBR anthraquinone dye, Trichoderma fungi can be used for bioremediation of such environments.
Źródło:
Archives of Environmental Protection; 2020, 46, 2; 12-20
2083-4772
2083-4810
Pojawia się w:
Archives of Environmental Protection
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Changes in the ascorbate-glutathione system during storage of recalcitrant seeds of Acer saccharinum L.
Autorzy:
Ratajczak, E
Pukacka, S.
Powiązania:
https://bibliotekanauki.pl/articles/57429.pdf
Data publikacji:
2006
Wydawca:
Polskie Towarzystwo Botaniczne
Tematy:
ascorbate-glutathione system
ascorbate peroxidase
storage
seed
Acer saccharinum
monodehydroascorbate reductase
Opis:
Two seed lots of Acer saccharinum (recalcitrant), with an initial moisture content of 50% and 55%, were stored at +3oC for 6 months. After this time, their viability (measured as germinability) reached 100% and 30%, respectively. In embryo axes and cotyledons extracted from seeds, two major low molecular antioxidants were assayed: ascorbate (ASA and DHA) and glutathione (GSH and GSSG); and activities of enzymes of the ascorbate-glutathione cycle were measured: ascorbate peroxidase (APO) (E.C. 1.11.1.11), monodehydroascorbate reductase (MR) (E.C. 1.6.5.4), dehydroascorbate reductase (DHAR) (E.C. 1.8.5.1), and glutathione reductase (GR) (E.C. 1.6.4.2.). GSH and GSSG contents of embryo axes of stored seeds decreased, as compared to the control (fresh, non-stored seeds), but a larger decrease was observed in seeds with 30% viability. In cotyledons, a particularly high increase in the GSH content in relation to the control was observed in seeds with 100% viability, while the GSSG content was significantly lower in both stored seed lots than in the control. The ASA level was twice as high in seeds with 30% viability as in the control, both in embryo axes and in cotyledons. The activity of enzymes of the ascorbate-glutathione cycle was higher in embryo axes than in cotyledons. In embryo axes of seeds with 100% viability, enzyme activities were slightly lower than in the control, while in those of seeds with 30% viability, their activities were higher than in the control. The observed changes in activities of enzymes of the ascorbate-glutathione cycle and in ascorbate and glutathione levels suggest that the stored seeds of A. saccharinum have an active antioxidant system, which plays an important role in maintaining their viability during storage.
Źródło:
Acta Societatis Botanicorum Poloniae; 2006, 75, 1; 23-27
0001-6977
2083-9480
Pojawia się w:
Acta Societatis Botanicorum Poloniae
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Wpływ kwasu siarkawego na reakcję utleniania kwasu L-askorbinowego przez peroksydazę w obecności nadtlenku wodoru
Vlijanie sernistojj kisloty na reakciju okislenija L-askorbinovojj kisloty dejjspleviem peroksidaza v prisutstvii perekosi vodorosta
Influence of sulfurous acid on oxidation of L-ascorbic acid by peroxidase in the presence of hydrogen peroxide
Autorzy:
Chmielnicka, J.
Powiązania:
https://bibliotekanauki.pl/articles/876744.pdf
Data publikacji:
1963
Wydawca:
Narodowy Instytut Zdrowia Publicznego. Państwowy Zakład Higieny
Tematy:
kwas L-askorbinowy
utlenianie
peroksydaza
nadtlenek wodoru
kwas siarkowy
L-ascorbic acid
oxidation
peroxidase
hydrogen peroxide
sulphuric acid
Źródło:
Roczniki Państwowego Zakładu Higieny; 1963, 14, 5
0035-7715
Pojawia się w:
Roczniki Państwowego Zakładu Higieny
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Changes in GSH-antioxidant system induced by daunorubicin in human normal and diabetic fibroblasts.
Autorzy:
Zatorska, Agnieszka
Maszewski, Janusz
Jóźwiak, Zofia
Powiązania:
https://bibliotekanauki.pl/articles/1043460.pdf
Data publikacji:
2003
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
glutathione S-transferase
glutathione reductase
glutathione peroxidase
daunorubicin
apoptosis
glutathione
oxidative stress
Opis:
We investigated the effect of daunorubicin on glutathione content and activity of GSH-related enzymes in cultured normal and diabetic human fibroblasts. Cells were incubated with 4 μM daunorubicin (DNR) for 2 h followed by culture in drug-free medium for up to 72 h. Treatment of diabetic cells with the drug caused a time-dependent depletion of intracellular GSH and a decrease of the GSH to total glutathione ratio. GSH depletion was accompanied by apoptotic changes in morphology of the nucleus. Analysis of GSH-related enzymes showed a significant increase of the activities of Se-dependent and Se-independent peroxidases and glutathione S-transferase. In contrast, glutathione reductase activity was reduced by 50%. Significant differences between normal and diabetic cells exposed to DNR were observed in the level of GST and Se-dependent glutathione peroxidase activities. These findings indicated that daunorubicin efficiently affects the GSH antioxidant defense system both in normal and diabetic fibroblasts leading to disturbances in glutathione content as well as in the activity of GSH-related enzymes.
Źródło:
Acta Biochimica Polonica; 2003, 50, 3; 825-835
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Glutathione-dependent responses of plants to drought: a review
Autorzy:
Labudda, M.
Azam, F.M.S.
Powiązania:
https://bibliotekanauki.pl/articles/58164.pdf
Data publikacji:
2014
Wydawca:
Polskie Towarzystwo Botaniczne
Tematy:
abiotic stress
plant response
glutathione peroxidase
glutathione S-transferase
reactive oxygen species
S-glutathionylation
water deficit
drought
Opis:
Water is a renewable resource. However, with the human population growth, economic development and improved living standards, the world’s supply of fresh water is steadily decreasing and consequently water resources for agricultural production are limited and diminishing. Water deficiency is a significant problem in agriculture and increasing efforts are currently being made to understand plant tolerance mechanisms and to develop new tools (especially molecular) that could underpin plant breeding and cultivation. However, the biochemical and molecular mechanisms of plant water deficit tolerance are not fully understood, and the data available is incomplete. Here, we review the significance of glutathione and its related enzymes in plant responses to drought. Firstly, the roles of reduced glutathione and reduced/ oxidized glutathione ratio, are discussed, followed by an extensive discussion of glutathione related enzymes, which play an important role in plant responses to drought. Special attention is given to the S-glutathionylation of proteins, which is involved in cell metabolism regulation and redox signaling in photosynthetic organisms subjected to abiotic stress. The review concludes with a brief overview of future perspectives for the involvement of glutathione and related enzymes in drought stress responses.
Źródło:
Acta Societatis Botanicorum Poloniae; 2014, 83, 1
0001-6977
2083-9480
Pojawia się w:
Acta Societatis Botanicorum Poloniae
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Effect of Medicago sativa Mhb1gene expression on defense response of Arabidopsis thaliana plants
Autorzy:
Maassen, Anna
Hennig, Jacek
Powiązania:
https://bibliotekanauki.pl/articles/1039900.pdf
Data publikacji:
2011
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
Arabidopsis thaliana
pathogen infection
nitrotyrosine
nitric oxide
peroxidase activity
non-symbiotic hemoglobin
Opis:
Besides the previously described nitric oxide-detoxification activity we identified new features of class-1 non-symbiotic hemoglobin from Medicago sativa (Mhb1). Under in vitro conditions, using peroxidase in-gel activity assay, the Mhb1 protein was shown to possess also peroxidase-like activity. Due to this activity, in the presence of nitrite and hydrogen peroxide, the protein can mediate autonitration and nitration of other proteins at tyrosine residues, as revealed by tandem mass spectrometry and immune assay approaches. Mhb1 through its multifunctional activities can affect different components of signal transduction cascades operating during plant response to infections. This influence is manifested by Mhb1-mediated selective up-regulation of expression of certain pathogen inducible genes in Pseudomonas syringae infected Arabidopsis thaliana plants which overproduce Mhb1, as revealed by reverse transcription-quantitative real-time PCR analysis. Changes in expression level of these genes can influence such processes as synthesis of secondary metabolites, protein degradation and biosynthesis of ethylene. They can also result in alteration of pathogen-induced defense response of Mhb1 transgenic plants.
Źródło:
Acta Biochimica Polonica; 2011, 58, 3; 427-432
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Application of TSA technique in localization of snRNP-rich cytoplasmic bodies
Autorzy:
Hyjek, M.
Wojciechowska, N.
Kolowerzo, A.
Smolinski, D.J.
Powiązania:
https://bibliotekanauki.pl/articles/80402.pdf
Data publikacji:
2013
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
conference
RNA maturation
eukaryotic cell
biogenesis
TSA technique
microsporocyte
catalytic activity
horseradish peroxidase
Źródło:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2013, 94, 3
0860-7796
Pojawia się w:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Profiling of plasma membrane bound peroxidases in maize (Zea mays L.)
Autorzy:
Perrineau, F.
Decker, D.
Luthje, S.
Powiązania:
https://bibliotekanauki.pl/articles/80819.pdf
Data publikacji:
2013
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
conference
plasma membrane
profiling
peroxidase
maize
Zea mays
heavy metal
cadmium
oxidative stress
reactive oxygen species
Źródło:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2013, 94, 2
0860-7796
Pojawia się w:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Functional analysis of peroxiredoxin isoforms in Euglena gracilis
Autorzy:
Tamaki, S.
Maruta, T.
Sawa, Y.
Shigeoka, S.
Ishikawa, T.
Powiązania:
https://bibliotekanauki.pl/articles/81160.pdf
Data publikacji:
2013
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
conference
Euglena gracilis
catalase
ascorbate peroxidase
cytosol
gene expression
peroxiredoxin
reactive oxygen species
functional analysis
Źródło:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2013, 94, 2
0860-7796
Pojawia się w:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Signalling via reactive oxygen species – why and how?
Autorzy:
Bartosz, G.
Powiązania:
https://bibliotekanauki.pl/articles/79914.pdf
Data publikacji:
2013
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
conference
signalling
reactive oxygen species
intracellular signalling
extracellular signalling
microorganism
animal
plant
hydrogen peroxide
protein tyrosine phosphatase
NADPH oxidase
cell wall
peroxidase
Źródło:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2013, 94, 2
0860-7796
Pojawia się w:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Aktywność peroksydazy i katalazy w liściach roślin owsa poddanych napromieniowaniu UV-B
Activity of peroxidase and catalase in the leaves of oat plants subjected to UV-B irradiation
Autorzy:
Lewandowski, R.
Skorska, E.
Powiązania:
https://bibliotekanauki.pl/articles/803950.pdf
Data publikacji:
2004
Wydawca:
Szkoła Główna Gospodarstwa Wiejskiego w Warszawie. Wydawnictwo Szkoły Głównej Gospodarstwa Wiejskiego w Warszawie
Opis:
W doświadczeniu wazonowym w szklarni przy dodatkowym napromieniowaniu UV-B (UV-BBE = 1,4 kJ·m⁻²·d⁻¹, 19 dni) badano reakcje roślin owsa (Avena sativa L.) należących do odmian tradycyjnych (Bajka, Dukat i Sprinter) oraz nagoziarnistych (Akt i Cacko). W napromieniowanych roślinach zaobserwowano zmiany aktywności peroksydazy w porównaniu z roślinami kontrolnymi: wzrost nastąpił w roślinach odmian Bajka, Dukat i Cacko. W roślinach nagoziarnistej odmiany Akt wyraźnie zmniejszyła się aktywność zarówno peroksydazy, jak i katalazy. Pod wpływem UV-B szerokość liści uległa niewielkiemu zwiększeniu. Nie stwierdzono istotnej zmiany zawartości barwników pochłaniających w zakresie UV-B (flawonoidów) wywołanej napromieniowaniem, chociaż zauważono niewielką tendencję wzrostową.
In the potted experiment with supplement UV-B irradiation (UV-BBE = 1.4 kJ·m⁻²·d⁻¹, 19 days) carried out in the glasshouse, the reaction of oat plants (Avena sativa L.) belonging to the husked varieties (Bajka, Dukat and Sprinter) and naked (Akt and Cacko) ones was studied. UV-B radiation caused changes of the peroxidase activity as compared to the control: an increase was observed in Bajka, Dukat and Cacko plants. Activity of peroxidase as well as catalase was reduced in plants of the naked variety Akt. UV-B radiation caused an increase of the leaf width in studied plants. Changes of the content of UV-B absorbing compounds (flavonoids) in irradiated plants were not confirmed, however, a slight increasing trend was observed.
Źródło:
Zeszyty Problemowe Postępów Nauk Rolniczych; 2004, 496, 2
0084-5477
Pojawia się w:
Zeszyty Problemowe Postępów Nauk Rolniczych
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Molecular forms of selected antioxidant enzymes in dog semen – electrophoretical identification
Autorzy:
Koziorowska-Gilun, M.
Strzezek, R.
Powiązania:
https://bibliotekanauki.pl/articles/32130.pdf
Data publikacji:
2011
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
molecular form
antioxidative enzyme
dog
semen
electrophoresis
identification
spermatozoon
enzyme
superoxide dismutase
catalase
glutathione peroxidase
reactive oxygen species
Opis:
The aim of the study was the electrophoretical identification of molecular forms of selected antioxidant enzymes in dog semen. Ejaculates to be studied were chosen from five dogs, aged from two to eight years. Polyacrylamide gel electrophoresis was carried out under non-denaturing conditions and then gels were stained for the activity of the following enzymes: superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx). Sperm homogenates and all fractions (pre-spermatic, spermatic and post-spermatic) of dog ejaculate demonstrated one protein band with SOD activity characterized by low electrophoretic mobility. Based on the confirmed sensitivity to H₂O₂, it can be assumed that the detected SOD is an enzyme containing ions of Zn²⁺ and Cu²⁺ (Cu,Zn SOD). In sperm homogenates one protein band with GPx activity was characterized by high electrophoretic mobility, whereas in the spermatic and post-spermatic fractions of dog ejaculate three protein bands with different (low, medium and high) electrophoretic mobility were identified. CAT molecular forms were not found in either sperm homogenates or in the analyzed fractions of ejaculate.
Źródło:
Polish Journal of Veterinary Sciences; 2011, 14, 1
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
pH Profile of cytochrome c-catalyzed tyrosine nitration
Autorzy:
Kambayashi, Yasuhiro
Hitomi, Yoshiaki
Kodama, Norio
Kubo, Masayuki
Okuda, Junna
Takemoto, Kei
Shibamori, Masafumi
Takigawa, Tomoko
Ogino, Keiki
Powiązania:
https://bibliotekanauki.pl/articles/1041220.pdf
Data publikacji:
2006
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
pseudo-peroxidase
pH
cytochrome c
nitrite
nitrotyrosine
Opis:
In the present study, we investigated how cytochrome c catalyzed the nitration of tyrosine at various pHs. The cytochrome c-catalyzed nitration of tyrosine occurred in proportion to the concentration of hydrogen peroxide, nitrite or cytochrome c. Thecytochromec-catalyzed nitration of tyrosine was inhibited by catalase, sodium azide, cystein, and uric acid. These results show that the cytochrome c-catalyzednitrotyrosine formation was due to peroxidase activity. The rate constant between cytochrome c and hydrogen peroxide within the pH range of 3 - 8 was the largest at pH 6 (37°C). The amount of nitrotyrosine formed was the greatest at pH 5. At pH 3, onlycytochromec-independent nitration of tyrosine occurred in the presence of nitrite. At this pH, the UV as well as visible spectrum of cytochrome c was changed by nitrite, even in the presence of hydrogen peroxide, probably via the formation of a heme iron - nitric oxide complex. Due to this change, the peroxidase activity of cytochrome c was lost.
Źródło:
Acta Biochimica Polonica; 2006, 53, 3; 577-584
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Selenium, glutathione and glutathione peroxidases in blood of patients with chronic liver diseases.
Autorzy:
Czuczejko, Jolanta
Zachara, Bronisław
Staubach-Topczewska, Ewa
Halota, Waldemar
Kędziora, Józef
Powiązania:
https://bibliotekanauki.pl/articles/1043405.pdf
Data publikacji:
2003
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
selenium
glutathione peroxidase
chronic liver disease
glutathione
oxidative stress
Opis:
Disturbances in the antioxidant system could play a role in pathogenesis of chronic liver disease. The aim of our study was to evaluate the levels/activities of antioxidants in blood of patients with chronic liver disease. We estimated selenium and glutathione concentrations and glutathione peroxidase activities in blood of 59 patients with chronic hepatitis B or C virus infection (group 1) and 64 patients with alcoholic, autoimmune or cryptogenic chronic liver disease (group 2). The results were compared with 50 healthy controls. Whole blood and plasma selenium and red cell glutathione concentrations were significantly lower in the patients compared with the controls. Red cell glutathione peroxidase activity was slightly reduced in both subgroups of group 1 and in group 2 with normal alanine aminotransferase values. Plasma glutathione peroxidase activity was slightly but significantly higher in patients with elevated aminotransferase values. The findings suggest that disturbances in antioxidant parameters in blood of patients with chronic liver disease may be the cause of the peroxidative damage of cells.
Źródło:
Acta Biochimica Polonica; 2003, 50, 4; 1147-1154
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Dysmutaza ponadtlenkowa, katalaza i peroksydaza glutationowa w trisomicznych fibroblastach ludzkich z hodowli in vitro
Activity of superoxide dismutase, catalase and glutathione peroxidase in hum an trisomie fibroblasts from in vitro culture
Autorzy:
Rózga, Błażej
Powiązania:
https://bibliotekanauki.pl/articles/944952.pdf
Data publikacji:
1998
Wydawca:
Uniwersytet Łódzki. Wydawnictwo Uniwersytetu Łódzkiego
Opis:
The activities of intercellular antioxidative enzymes: Superoxide dismutase, catalase and glutathione peroxidase were estimated in control and trisomic human fibroblasts in culture. It was found that in trisomic cells the activity of SOD is approximately 50% higher than in control cells. The activity of glutathione peroxidase is increased by 30% in trisomic fibroblasts, whereas catalase activity is similar in both types of cells.
Źródło:
Acta Universitatis Lodziensis. Folia Biochimica et Biophysica; 1998, 13
0208-614X
Pojawia się w:
Acta Universitatis Lodziensis. Folia Biochimica et Biophysica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Examining organic acid root exudate content and function for leafy vegetables under water-stressed conditions
Autorzy:
Kasukawa, N.
Miyazawa, K.
Powiązania:
https://bibliotekanauki.pl/articles/1077275.pdf
Data publikacji:
2020
Wydawca:
Instytut Ogrodnictwa
Tematy:
leaf vegetable
cabbage
abiotic stress
drought stress
organic acid
root exudate
antioxidant enzyme
catalase
ascorbate peroxidase
Opis:
At the plants’ exposal to abiotic stress, organic acids, including citric acid, are exuded through their roots. Previous studies have suggested that the exogenous application of citric acid increases antioxidant activity within the plant. Thus, we postulated that organic acids released into the surroundings during times of environmental stress may function as signaling molecules to increase antioxidant enzyme activity. To gain further insight into this phenomenon, we identified individual organic acids exuded from the roots of leafy vegetables under drought stress. We then analyzed enzyme activity and the root/shoot lengths of seedlings after treatment with the types of organic acids found to be exuded from the studied leafy vegetables, including acetic, citric, lactic, and tartaric acids. There was a significant increase in catalase and ascorbate peroxidase enzyme activity in Napa cabbage (Brassica rapa var. pekinensis) after exogenous citric acid application. Root lengths of cabbage (Brassica oleracea var. capitata) and Napa cabbage seedlings were significantly longer in citric and lactic acids pretreated seedlings compared to those of the control. The above results support the conclusion that exogenous application of citric acid alleviates drought stress. However, there is insufficient evidence to prove that organic acids act as signaling molecules to prime neighboring plants for upcoming stress.
Źródło:
Journal of Horticultural Research; 2020, 28, 2; 83-90
2300-5009
Pojawia się w:
Journal of Horticultural Research
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Effect of ethylene antagonist silver thiosulphate on the flower longevity of Clarkia pulchella Pursh.
Autorzy:
Dar, R.A.
Tahir, I.
Powiązania:
https://bibliotekanauki.pl/articles/1920.pdf
Data publikacji:
2018
Wydawca:
Instytut Ogrodnictwa
Tematy:
silver thiosulphate
flower longevity
Clarkia pulchella
bud
antioxidant
catalase
lipoxygenase
superoxide dismutase
lipid peroxidation
ascorbate peroxidase
Źródło:
Journal of Horticultural Research; 2018, 26, 1
2300-5009
Pojawia się w:
Journal of Horticultural Research
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Antioxidants and allergenic proteins as compounds produced by spice plants under stress
Autorzy:
Slowianek, M.
Leszczynska, J.
Powiązania:
https://bibliotekanauki.pl/articles/80246.pdf
Data publikacji:
2013
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
conference
antioxidant
allergenic protein
spice plant
stress
reactive oxygen species
superoxide dismutase
catalase
peroxidase
allergic reaction
plant allergen
Źródło:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2013, 94, 3
0860-7796
Pojawia się w:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The reactive nitrogen species affect potato immunity to Phytophthora infestans
Autorzy:
Arasimowicz-Jelonek, M.
Floryszak-Wieczorek, J.
Abramowski, D.
Izbianska, K.
Powiązania:
https://bibliotekanauki.pl/articles/81084.pdf
Data publikacji:
2013
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
conference
reactive nitrogen species
nitric oxide
peroxynitrite
potato
Phytophthora infestans
gene coding
thioredoxin peroxidase
Źródło:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2013, 94, 3
0860-7796
Pojawia się w:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Glutathione and GSH-dependent enzymes in patients with liver cirrhosis and hepatocellular carcinoma
Autorzy:
Czeczot, Hanna
Ścibior, Dorota
Skrzycki, Michał
Podsiad, Małgorzata
Powiązania:
https://bibliotekanauki.pl/articles/1041298.pdf
Data publikacji:
2006
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
malondialdehyde
glutathione S-transferase
glutathione reductase
liver cirrhosis
glutathione peroxidase
glutathione
hepatocellular carcinoma
Opis:
We investigated glutathione level, activities of selenium independent GSH peroxidase, selenium dependent GSH peroxidase, GSH S-transferase, GSH reductase and the rate of lipid peroxidation expressed as the level of malondialdehyde in liver tissues obtained from patients diagnosed with cirrhosis or hepatocellular carcinoma. GSH level was found to be lower in malignant tissues compared to adjacent normal tissues and it was higher in cancer than in cirrhotic tissue. Non-Se-GSH-Px activity was lower in cancer tissue compared with adjacent normal liver or cirrhotic tissue, while Se-GSH-Px activity in cancer was found to be similar to its activity in cirrhotic tissue and lower compared to control tissue. An increase in GST activity was observed in cirrhotic tissue compared with cancer tissue, whereas the GST activity in cancer was lower than in adjacent normal tissue. The activity of GSH-R was similar in cirrhotic and cancer tissues, but higher in cancer tissue compared to control liver tissue. An increased level of MDA was found in cancer tissue in comparison with control tissue, besides its level was higher in cancer tissue than in cirrhotic tissue. Our results show that the antioxidant system of cirrhosis and hepatocellular carcinoma is severely impaired. This is associated with changes of glutathione level and activities of GSH-dependent enzymes in liver tissue. GSH and enzymes cooperating with it are important factors in the process of liver diseases development.
Źródło:
Acta Biochimica Polonica; 2006, 53, 1; 237-242
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Wpływ substancji konserwujących żywność na aktywność katalazy i peroksydazy roślinnej oraz zwierzęcej. Część II. Badanie wpływu benzoesanu sodowego i sorbinianu potasowego na aktywność katalazy i peroksydazy krwi szczura
The effect of food preservatives on the acttvity of animal and vegetable catalase and peroxidase Part. II. Etudy of the effects of sodium benzoate and potassium sorbate on the activity of blood catalase and peroxidase in rat
Autorzy:
Lemieszek-Chodorowska, K.
Powiązania:
https://bibliotekanauki.pl/articles/873263.pdf
Data publikacji:
1973
Wydawca:
Narodowy Instytut Zdrowia Publicznego. Państwowy Zakład Higieny
Tematy:
zywnosc
katalaza
benzoesan sodu
sorbinian potasu
krew
szczur
zwierzeta doswiadczalne
Źródło:
Roczniki Państwowego Zakładu Higieny; 1973, 24, 5
0035-7715
Pojawia się w:
Roczniki Państwowego Zakładu Higieny
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Aspergillus niger, a dominant phylloplane coloniser, influences the activity of defense enzymes in Solanum lycopersicum
Autorzy:
Goswami, S.
Paul, P.K.
Sharma, P.D.
Powiązania:
https://bibliotekanauki.pl/articles/2084798.pdf
Data publikacji:
2019
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
Aspergillus niger
phylloplane
peroxidase
phenylalanine ammonia lyase
polyphenol oxidase
Solanum lycopersicum
tyrosine ammonia lyase
Opis:
Phylloplane microbes have been studied as strategic tools in management against plant pathogens. Non-pathogenic bacteria and fungi have been applied as crop protectants against various plant diseases. The present study aimed at evaluating the potentiality of Aspergillus niger spores in altering the activity of four key enzymes related to defense in tomato. The experiment was designed such that two groups of 50 tomato plants were considered: group 1 – sprayed with autoclaved distilled water (control) and group 2 – sprayed with A. niger spores. Spraying was carried out under aseptic conditions. The experimental parameters included analysis of the activity of peroxidase (POX), polyphenol oxidase (PPO), phenylalanine ammonia lyase (PAL) and tyrosine ammonia lyase (TAL) as well as expression of POX and PPO isoforms. The results demonstrated an inductive effect of A. niger on the activity of POX, PPO, PAL and TAL. Enhanced expression of POX and PPO isoforms was also observed. The results indicated that A. niger can be considered probiotic for the management of tomato against its phytopathogens.
Źródło:
Journal of Plant Protection Research; 2019, 59, 4; 512-518
1427-4345
Pojawia się w:
Journal of Plant Protection Research
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Antioxidant defence system in the leaves and roots of A. thaliana under long-term S deficiency
Autorzy:
Ostaszewska, M.
Juszczuk, I.M.
Powiązania:
https://bibliotekanauki.pl/articles/79954.pdf
Data publikacji:
2013
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
conference
antioxidant defense system
Arabidopsis thaliana
leaf
root
photosynthetic pigment
carotenoid
superoxide dismutase
ascorbate peroxidase
Źródło:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2013, 94, 3
0860-7796
Pojawia się w:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Silica nanoparticles enhances physio-biochemical characters and postharvest quality of Rosa hybrida L. cut flowers
Autorzy:
El-Serafy, R.S.
Powiązania:
https://bibliotekanauki.pl/articles/1961.pdf
Data publikacji:
2019
Wydawca:
Instytut Ogrodnictwa
Tematy:
silica nanoparticle
postharvest quality
Rosa hybrida
biochemical characteristics
membrane stability index
phenol
flavonoids
malondialdehyde
peroxidase
polyphenol oxidase
Źródło:
Journal of Horticultural Research; 2019, 27, 1
2300-5009
Pojawia się w:
Journal of Horticultural Research
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Activity of selected antioxidant enzymes in Quercus robur L. leaves infected with Microsphaera alphitoides
Autorzy:
Skwarek, M.
Patykowski, J.
Nowogorska, A.
Powiązania:
https://bibliotekanauki.pl/articles/80038.pdf
Data publikacji:
2013
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
conference
antioxidative enzyme
Quercus robur
leaf infection
Microsphaera alphitoides
powdery mildew
oak powdery mildew
Ascomycetes
peroxidase
superoxide dismutase
Źródło:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2013, 94, 3
0860-7796
Pojawia się w:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Antioxidant supplementation to medium for in vitro embryo production in Felis catus
Autorzy:
Cocchia, N.
Tafuri, S.
Del Prete, C.
Palumbo, V.
Esposito, L.
Avallone, L.
Ciani, F.
Powiązania:
https://bibliotekanauki.pl/articles/2087464.pdf
Data publikacji:
2019
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
assisted reproduction technology
superoxide dismutase
glutathione peroxidase
catalase
domestic cat
Źródło:
Polish Journal of Veterinary Sciences; 2019, 3; 573-579
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Anti-thyroidal peroxidase antibodies are associated with thyrotropin levels in hypothyroid patients and in euthyroid individuals
Autorzy:
Bromińska, Barbara
Bromiński, Gabriel
Owecki, Maciej
Michalak, Michał
Czarnywojtek, Agata
Waśko, Ryszard
Ruchała, Marek
Powiązania:
https://bibliotekanauki.pl/articles/989550.pdf
Data publikacji:
2017
Wydawca:
Instytut Medycyny Wsi
Tematy:
anti-tpo
autoimmunity
hypothyroidism
euthyroidism
tsh
thyreoperoxidase
Opis:
Objective. The study was designed to evaluate the relationship between thyroid antibodies and gland dysfunction, with the aim of finding a clinically useful threshold value of thyreoperoxidase antibodies, which could prove to be predictive for thyroid failure. Materials and method. The study was conducted on 99 women, ages ranging from 18–91 years (mean age: 45.5 ±17.0), were treated as outpatients in the Department of Endocrinology, Metabolism and Internal Medicine. Analysis of serum samples for TSH concentration and anti-TPO titers was conducted. Results. The most common disorder was hypothyroidism. Anti-TPO titers above reference range values were observed in 35 patients (35.4%): 21 (60%) were hypothyroid and 11 (31.4 %) were euthyroid. The anti-TPO and TSH serum levels correlated both in patients with high thyroid antibody titers, and in the anti-TPO negative groups. To find the threshold value of anti-TPO that would help predict hypothyroidism, receiver operating curves were used. With this approach, TPO antibody titers over 17 IU/ml indicated hypothyroidism with a 90% sensitivity and 75% sensibility. Conclusion. It can be postulated that the cutoff values of anti-TPO in the general population should be decreased in order to improve autoimmune thyroid disorder screening. Obviously, using that margin may lead initially to the detection of some false positive subjects. However, with lower cut-off values, more patients can be enrolled into thyroid follow-up groups. In this way, many people could avoid complications of undiagnosed, insidious thyroid failure.
Źródło:
Annals of Agricultural and Environmental Medicine; 2017, 24, 3
1232-1966
Pojawia się w:
Annals of Agricultural and Environmental Medicine
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Markers of physiological stress in common pea (Pisum sativum) in response to soil contamination with tetracycline
Autorzy:
Ziolkowska, A.
Dobiesz, M.
Piotrowicz-Cieslak, A.I.
Adomas, B.
Powiązania:
https://bibliotekanauki.pl/articles/80121.pdf
Data publikacji:
2013
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
conference
physiological stress
common pea
Pisum sativum
soil contamination
tetracycline
peroxidase activity
carbohydrate
Źródło:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2013, 94, 3
0860-7796
Pojawia się w:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Effects of NaCl, CaCl2 and their combination of salt on seed germination and seedling growth of Lycopersicon esculentum L.
Autorzy:
Sivasankaramoorthy, S.
Powiązania:
https://bibliotekanauki.pl/articles/11521.pdf
Data publikacji:
2014
Wydawca:
Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
Tematy:
sodium chloride
calcium chloride
chlorophyll
catalase
peroxidase
salt
seed germination
seedling growth
Lycopersicon esculentum
tomato
salt stress
Opis:
To compare the effect of NaCl, CaCl2 and their combinations on germination and early seedling growth stages of Lycopersicum esculentum L., were studied under pot experiments 2008. Results indicated that significant increases were recorded in percentage of germination, seedling fresh and dry weights, seedling length, water content, catalase activity and photosynthetic pigments (chlorophyll a, b and total chlorophylls as well as carotenoids) under the low level concentration (20 mM) of NaCl or CaCl and their combination (1:1). On other hand increasing salt concentration in nutrient solution caused significant decrease in all of these parameters. The great reduction occurred under high salinity level of NaCl (50 mM). Meanwhile, peroxidase activity increased significantly with increasing salinity levels from 20 mM to 50 mM of both applied salinity types. Besides, peroxidase activity under NaCl salinity showed a marked increase followed by NaCl + CaCl2 (1:1) and CaCl2 at 50 mM.
Źródło:
International Letters of Natural Sciences; 2014, 17
2300-9675
Pojawia się w:
International Letters of Natural Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Activity of selected components of antioxidant system in grass pea and yellow lupine protoplasts after enzymatic isolation
Autorzy:
Wiszniewska, A.
Piwowarczyk, B.
Powiązania:
https://bibliotekanauki.pl/articles/80679.pdf
Data publikacji:
2015
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
antioxidant
phenolic compound
grass pea
Lathyrus sativus
yellow lupin
Lupinus luteus
peroxidase
radical scavenging activity
oxidative stress
Źródło:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2015, 96, 4
0860-7796
Pojawia się w:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Biocontrol mechanisms of Trichoderma harzianum against soybean charcoal rot caused by Macrophomina phaseolina
Autorzy:
Khaledi, N.
Taheri, P.
Powiązania:
https://bibliotekanauki.pl/articles/65311.pdf
Data publikacji:
2016
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
biological control
Trichoderma harzianum
soybean
charcoal rot disease
soil-borne disease
stem disease
root disease
Macrophomina phaseolina
peroxidase
phenol
total phenol
Opis:
Throughout the world, charcoal rot, caused by Macrophomina phaseolina, is one of the most destructive and widespread diseases of crop plants such as soybean. In this study, the biological control capability of 11 Trichoderma spp. isolates against M. phaseolina was investigated using screening tests. Among all the tested Trichoderma spp. isolates, inhibition varied from 20.22 to 58.67% in dual culture tests. Dual culture, volatile and non-volatile tests revealed that two isolates of Trichoderma harzianum (including the isolates T7 and T14) best inhibited the growth of M. phaseolina in vitro. Therefore, these isolates were selected for biocontrol of M. phaseolina in vivo. The results of greenhouse experiments revealed that disease severity in the seed treatment with T. harzianum isolates was significantly lower than that of the soil treatment. In most of the cases, though, soil treatment with T. harzianum resulted in higher plant growth parameters, such as root and shoot weight. The effects of T. harzianum isolates on the activity of peroxidase enzyme and phenolic contents of the soybean root in the presence and absence of M. phaseolina were determined in greenhouse conditions. Our results suggested that a part of the inhibitory effect of T. harzianum isolates on soybean charcoal rot might be related to the indirect influence on M. phaseolina. Plant defense responses were activated as an elicitor in addition to the direct effect on the pathogen growth.
Źródło:
Journal of Plant Protection Research; 2016, 56, 1
1427-4345
Pojawia się w:
Journal of Plant Protection Research
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The role of ascorbate during cadmium-induced oxidative stress in Arabidopsis thaliana
Autorzy:
Bielen, A.
Remans, T.
Vangronsveld, J.
Cuypers, A.
Powiązania:
https://bibliotekanauki.pl/articles/79901.pdf
Data publikacji:
2013
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
conference
cadmium
oxidative stress
reactive oxygen species
cellular level
antioxidant defense system
stress condition
ascorbate peroxidase
Arabidopsis thaliana
ascorbate-glutathione cycle
Źródło:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2013, 94, 2
0860-7796
Pojawia się w:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Redox and nitric oxide (NO) homeostasis differentially regulated in tomato roots and leaves under salinity stress
Autorzy:
Manai, J.
Gouia, H.
Corpas, F.
Powiązania:
https://bibliotekanauki.pl/articles/80844.pdf
Data publikacji:
2013
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
conference
nitric oxide
redox
homeostasis
tomato
Solanum lycopersicum
root
leaf
salinity stress
plant growth
S-nitrosoglutathione
glutathione peroxidase
Źródło:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2013, 94, 2
0860-7796
Pojawia się w:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Effect of phenolics from woody plants on activity of grain aphid oxidases
Autorzy:
Chrzanowski, G.
Leszczynski, B.
Sempruch, C.
Sytykiewicz, H.
Sprawka, I.
Powiązania:
https://bibliotekanauki.pl/articles/54949.pdf
Data publikacji:
2009
Wydawca:
Sieć Badawcza Łukasiewicz - Instytut Przemysłu Organicznego
Tematy:
sour cherry
walnut
peroxidase
leaf
woody plant
biopesticide
phenolics
plant pest
polyphenol oxidase
grain aphid
black currant
aphid
Opis:
Changes in activity of the grain aphid peroxidase (Px) and polyphenol oxidase (PPO) towards phenolics isolated from leaves of black currant, sour cherry and walnut were examined. Slight increase in activity of peroxidase was found within insect tissues after 24 h of walnut extract treatment, whereas black currant and sour cherry strongly inhibited activity of this enzyme. Later on, the walnut extract reduced activity of the enzyme, finally about 30%. The other extracts showed slight changes in Px activity. The grain aphid’s polyphenol oxidase was stimulated during the first 24 h of the experiment. Further treatment with the phenolics extracts reduced activity of the grain aphid polyphenol oxidase. Generally, phenolics isolated from the black currant and sour cherry were more effective in reducing activity of the aphid peroxidase, whereas phenolics from walnut reduced the polyphenol oxidase activity. Possible application of the phenolics isolated from the woody plants as modern biopesticides towards the grain aphid is discussed.
Źródło:
Pestycydy; 2009, 1-4; 63-70
0208-8703
Pojawia się w:
Pestycydy
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Prediction of indels and SNP’s in coding regions of glutathione peroxidases - an important enzyme in redox homeostasis of plants
Autorzy:
Ganguli, S.
Datta, A.
Powiązania:
https://bibliotekanauki.pl/articles/11303.pdf
Data publikacji:
2014
Wydawca:
Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
Tematy:
prediction
indel
single nucleotide polymorphism
coding region
glutathione peroxidase
enzyme
redox
homeostasis
plant
genotype
stress tolerance
Opis:
Plant glutathione peroxidases are an important class of enzymes which play key roles in the stress adaptability of plants both in context of biotic and abiotic stress pathways. They have been over the years much studied in animals since the catalytic residues are comprised of selenocysteine a variant amino acid which is ribosomally encoded with the help of an RNA structural element known as SECIS. Various workers over the years have shown that plant glutathione peroxidases play active roles in ROS sequestration, lipid hydroperoxidation as well as regulate glutathione levels. However, each plant has various patterns of glutathione peroxidase expression and action and in some plants certain isoforms have not been detected at all. This work focuses on the prediction and identification of single nucleotide polymorphisms (SNPs) and INDELs in the coding regions of plant glutathione peroxidases, with the help of a Bayesian based algorithm subsequently validated. A large number of informative sites were detected 279 of which had variant frequency of ≥ 50 %. This data should be beneficial for future studies involving genetic manipulation and population based breeding experiments.
Źródło:
International Letters of Natural Sciences; 2014, 02
2300-9675
Pojawia się w:
International Letters of Natural Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Superoxide dismutase and glutathione peroxidase activity in porcine follicular fluid i relation to follicle size, birth status of gilts, ovarian location and year season
Aktywnosc dyzmutazy ponadtlenkowej i peroksydazy glutationowej w swinskim plynie pecherzykowym w odniesieniu do wielkosci pecherzyka, statusu porodowego loch, polozenia jajnika i pory roku
Autorzy:
Lasota, B
Blaszczyk, B.
Stankiewicz, T.
Udala, J.
Szewczyk, M.
Matusiak-Bielska, D.
Powiązania:
https://bibliotekanauki.pl/articles/45154.pdf
Data publikacji:
2009
Wydawca:
Zachodniopomorski Uniwersytet Technologiczny w Szczecinie. Wydawnictwo Uczelniane ZUT w Szczecinie
Tematy:
pig
birth status
oxidative metabolism
embryo energy production
reactive oxygen species
mammal
oocyte
embryo
follicular fluid
porcine oocyte
oxidative stress
glutathione peroxidase
superoxide dismutase
porcine follicular fluid
season
ovarian location
gilt
follicle size
Źródło:
Acta Scientiarum Polonorum. Zootechnica; 2009, 08, 1-2; 19-29
1644-0714
Pojawia się w:
Acta Scientiarum Polonorum. Zootechnica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Utilization of selenium compounds in nutrition of lambs
Wykorzystanie zwiazkow selenu w zywieniu jagniat
Autorzy:
Slupczynska, M
Kinal, S.
Hadrys, M.
Krol, B.
Powiązania:
https://bibliotekanauki.pl/articles/14395.pdf
Data publikacji:
2009
Wydawca:
Uniwersytet Warmińsko-Mazurski w Olsztynie / Polskie Towarzystwo Magnezologiczne im. Prof. Juliana Aleksandrowicza
Tematy:
animal feeding
lamb
selenium compound
utilization
bioavailability
tissue accumulation
glutathione peroxidase
feed mixture
selenium supplementation
chemical form
Opis:
The level of mineral utilization depends on many factors related to animals as well as to the chemical form of given nutrients. It has been experimentally demonstrated that animals utilize inorganic forms of minerals less efficiently than organic ones. Hence, an attempt has been made to evaluate the bioavailability of selenium bound in different compounds to lambs. Selenium supplementation in fodder mixtures was another aspect included in our tests. Thus, an experiment was conducted on 48 growing lambs, testing the level and chemical forms of selenium in fodder mixtures for animals. In group I (the control) lambs received fodder mixture without any selenium supplement. In the experimental groups, selenium was supplemented as sodium selenite in the amount 0.2 mg Se kg-1 d.m. feeds (II), or selenium enriched yeast (Se-yeast) in the amounts of 0.1 and 0.2 mg Se kg-1 d.m. of feed groups III and IV, respectively. At the end of the experiment, when animals had reached 35 kilos of weight, blood samples were taken. The activity of glutathione peroxidase was estimated in heparinized blood samples. Eight lambs were chosen from each group and killed to collect samples of soft tissues (liver, kidney, brain, muscle). The content of selenium was determined in the tissue samples. Supplementation of feeds for lambs with selenium, both inorganic (sodium selenite) and organic (Se-yeast), increased the content of the element in soft tissues of animals. The highest level of the element was found in the liver and kidneys: 4.65 and 4.90 and 2.10 and 2.30 mg kg-1 fresh tissue, of the lambs receiving sodium selenine and Se-yeast in the amount 0.2 mg Se kg-1 D.M. of feed (groups II and IV), respectively. Selenium compounds added to feeds given to lambs significantly (P£0.01) increased activity of GSH-Px in blood, especially in the case of animals which received yeast enriched with selenium. In blood of the lambs which received feeds with Se-yeast in the amount 0.2 mg Se kg-1 d.m. of feed (group IV), the activity of glutathione peroxidase was by 129.71 U gHb-1 higher, and of the animals which received Se-yeast in the amount 0.1 mg kg-1 d.m. or sodium selenite, the activity of the enzyme was higher by 86.33 and 86.35 U gHb-1, respectively, than the activity of this enzyme in blood of animals from the control group. Supplementation of lambs’ rations with Se in the form of selenite or yeast enriched with selenium forms increased the content of Se in soft tissues and gluthatione peroxidase activity in comparison with animals which did not receive additional doses of this nutrient in fodder mixtures. The availability of Se was more profoundly affected by the amount of the element added rather than its form.
Stopień wykorzystania składników mineralnych z paszy zależy od wielu czynników związanych zarówno ze zwierzęciem, jak i z formą chemiczną stosowanych związków. W badaniach wskazano, że związki nieorganiczne są najczęściej gorzej wykorzystywane niż ich organiczne odpowiedniki. Dlatego podjęto próbę oceny przyswajalności przez jagnięta różnych związków selenu, uwzględniając także poziom dodatku tego pierwiastka w mieszankach. W badaniach na 48 rosnących jagniętach czynnikiem różnicującym poszczególne grupy były różne poziomy i formy połączeń chemicznych selenu w mieszankach treściwych. W grupie I (kontrolnej) jagnięta otrzymywały mieszankę bez dodatku selenu. W grupach (II) doświadczalnych mieszanki uzupełniano seleninem sodu w ilości 0.2 mg Se kg-1 s.m. paszy, a w grupach III i IV – drożdżami wzbogaconymi w selen (drożdże-Se), odpowiednio w ilości: 0.1 i 0.2 mg Se kg-1 s.m. paszy. Na końcu doświadczenia, po osiągnięciu przez zwierzęta masy ciała ok. 35 kg, pobrano od nich krew. Następnie w pełnej heparynizowanej krwi oznaczono aktywność peroksydazy glutationowej (GSH-Px). Z każdej grupy wybrano losowo po 8 jagniąt, zwierzęta ubito i pobrano tkanki miękkie (wątroba, nerki, mózg, mięsień przywodziciel uda). W zliofilizowanym materiale biologicznym oznaczono zawartość selenu. Podane jagniętom w mieszankach treściwych zarówno nieorganiczne (selenin sodu), jak i organiczne (drożdże-Se) formy połączeń selenu zwiększyły zawartość tego pierwiastka w tkankach miękkich jagniąt. Najwięcej selenu znajdowało się w wątrobie i nerkach, odpowiednio 4.65 i 4.90 oraz 2.10 i 2.30 mg kg-1 świeżej tkanki jagniąt otrzymujących w paszy selenin sodu i drożdże–Se w ilości 0.2 mg Se kg-1 s.m. paszy (gr. II i IV). Stosowane w paszy jagniąt związki selenu wyraźnie (P≤0.01) zwiększyły aktywności GSH-Px we krwi owiec, zwłaszcza u zwierząt, które w mieszance otrzymywały drożdże wzbogacone w selen. U owiec otrzymujących w paszy drożdże-Se w ilości 0.2 mg Se kg-1 s.m. paszy (grupa IV) wykazano wyższą o 129.71 U gHb-1 aktywność peroksydazy glutationowej, a u zwierząt, którym w paszy podawano drożdże-Se w ilości 0.1 mg Se kg-1 s.m. paszy lub selenin sodu, aktywność tego enzymu we krwi była wyższa odpowiednio o 86.33 i 86.35 U gHb-1 w porównaniu z grupą kontrolną. Zastosowanie w dawkach dla jagniąt dodatku Se, zarówno w formie seleninu, jak i wzbogacanych drożdży, spowodowało wzrost jego zawartości w tkankach miękkich oraz wzrost aktywności peroksydazy glutationowej w porównaniu ze zwierzętami z grupy kontrolnej. Większy wpływ na dostępność Se miał jego poziom w dawce niż stosowana forma.
Źródło:
Journal of Elementology; 2009, 14, 1; 157-163
1644-2296
Pojawia się w:
Journal of Elementology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The chloroplast thiol-disulphide redox regulatory network and the sensory roles of 2-cysteine peroxiredoxin and cyclophilin 20-3
Autorzy:
Dietz, K.
Powiązania:
https://bibliotekanauki.pl/articles/80178.pdf
Data publikacji:
2013
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
conference
regulatory network
reactive oxygen species
reactive nitrogen species
chloroplast
peroxiredoxin
2-cysteine peroxiredoxin
peroxidase
chaperone
thiol
thiol-disulphide redox
Źródło:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2013, 94, 2
0860-7796
Pojawia się w:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The effects of soybean selenium proteinate on tissue selenium and meat quality traits in finishing pigs
Autorzy:
Svoboda, M.
Fajt, Z.
Banoch, T.
Salakova, A.
Drabek, J.
Powiązania:
https://bibliotekanauki.pl/articles/32132.pdf
Data publikacji:
2011
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
meat quality
pig
quality trait
selenium
sodium selenite
soybean
tissue
pork
glutathione peroxidase
muscle
Opis:
The purpose of the present study was to evaluate the effects of soybean selenium proteinate on Se tissue retention and meat quality in pigs. In group A (n=11) the mixtures were supplemented with soybean selenium proteinate, in group B (n=11) with sodium selenite and in group C (n=11) with Se-enriched yeast (0.3 mg Se per kg in all groups). The use of soybean selenium proteinate resulted in lower retention of Se in tissues (liver, heart, muscle) compared to Se-enriched yeast. Selenium concentrations in tissues achieved by soybean selenium proteinate and sodium selenite were comparable. No differences in serum Se, serum GSH-Px and meat quality traits were found among the groups.
Źródło:
Polish Journal of Veterinary Sciences; 2011, 14, 1
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Aktywnosc beta-glikozydazy i peroksydazy w siewkach lubinu zoltego infekowanych Fusarium oxysporum f. sp. lupini
Beta-glycosidase and peroxidase activity in yellow lupin seedling infected by Fusarium oxysporum f. sp. lupini
Autorzy:
Rybus-Zajac, M
Morkunas, I
Powiązania:
https://bibliotekanauki.pl/articles/27357.pdf
Data publikacji:
2005
Wydawca:
Polskie Towarzystwo Botaniczne
Tematy:
zamieranie roslin
beta-glikozydaza
aktywnosc enzymatyczna
choroby grzybowe
enzymy
infekcja grzybowa
gnicie korzeni
Fusarium oxysporum f.sp.lupini
lubin zolty
mechanizmy obronne
choroby roslin
zgorzel przedwschodowa
zmiany morfologiczne
peroksydaza
siewki
grzyby chorobotworcze
plant decline
beta-glycosidase
enzyme activity
fungal disease
enzyme
fungal infection
root decay
yellow lupin
defense mechanism
plant disease
morphological change
peroxidase
seedling
pathogenic fungi
Opis:
Lupine diseases caused by pathogenic fungi constitute a serious problem in agriculture. They lead to partial yield loss and deterioration of crop quality through the changes in biochemical composition of seeds or their contamination with mycotoxins. Some of common lupine diseases are fusarioses caused by Fusarium oxyspomm f. sp. lupini. Morphometric and metabolic changes were investigated in yellow lupine seedlings infected with F. oxyspomm f. sp. lupini. It was found that infection caused temporary inhibition of seedling growth, overcome at later development, and activation of ß-glycosidase and peroxidases. The changes in enzymes activity indicate the induction of defense mechanism against F. oxyspomm f. sp. lupini and inhibition of pathogen spread.
Choroby łubinu powodowane przez grzyby chorobotwórcze stanowią poważny problem w rolnictwie. Prowadzą do zniszczenia części plonu oraz pogorszenia jego jakości, poprzez zmiany w składzie biochemicznym nasion lub zakażenie ich mykotoksynami. Do powszechnie występujących chorób łubinu zaliczane są fuzariozy powodowane przez Fusarium oxysporum f. sp. lupini. Badano zmiany morfologiczne oraz metaboliczne w siewkach łubinu żółtego infekowanych F. oxysporum f. sp. lupini. Stwierdzono, że infekcja spowodowała okresowe zahamowanie wzrostu siewek, przełamane w dalszej fazie ich rozwoju oraz aktywację ß-glikozydazy oraz peroksydaz. Poinfekcyjne zmiany aktywności enzymów wskazują na uruchamianie mechanizmu obronnego względem F. oxysporum f. sp. lupini i zahamowanie rozprzestrzeniania się patogena.
Źródło:
Acta Agrobotanica; 2005, 58, 2; 103-110
0065-0951
2300-357X
Pojawia się w:
Acta Agrobotanica
Dostawca treści:
Biblioteka Nauki
Artykuł
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