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Tytuł:
Determination of Pleurotus abieticola ligninolytic activity on norway spruce wood
Autorzy:
Żółciak, Anna
Powiązania:
https://bibliotekanauki.pl/articles/2041965.pdf
Data publikacji:
2019
Wydawca:
Instytut Badawczy Leśnictwa
Tematy:
laccase
lignin peroxidase
manganese peroxidase
versatile peroxidase
spruce
white rot fungus
wood
Opis:
The effect of Phlebiopsis gigantea treatment in control of Heterobasidion parviporum in Norway spruce is less effective than that in control of Heterobasidion annosum in pine. It is necessary to apply other fungi, for example, Pleurotus abieticola in Norway spruce stands. Thus, it is necessary to assess the activity of major ligninolytic enzymes, that is, laccase, lignin peroxidase (LiP), manganese peroxidase (MnP) and versatile peroxidase (VP) produced by P. abieticola, which may be effective in the fast degradation of Norway spruce wood. Three strains of P. abieticola (Pa1-3) were grown on pieces of Norway spruce sapwood and heartwood for 50 days in laboratory conditions. Enzymatic activity was determined using spectrophotometry. Pleurotus abieticola produced laccase, LiP, MnP and VP. The activity of laccase was low, ranging 0–3.696 and 0–0.806 mU/μg of protein in sapwood and heartwood, respectively. The highest activity in Pa1 = 3.696 mU/μg of protein in sapwood and in Pa3 = 0.806 mU/μg of protein in heartwood was observed after 30 and 50 days of incubation, respectively. The activity of LiP was also low, ranging 0–0.188 and 0–0.271 mU/μg of protein in sapwood and heartwood, respectively. The highest activity in Pa1 = 0.271 mU/μg of protein in sapwood and in Pa2 = 0.188 mU/μg of protein in heartwood was observed after 40 and 20 days of incubation, respectively. The activity of MnP ranged 0–17.618 and 0–12.203 mU/μg of protein in sapwood and heartwood, respectively. This enzymatic activity peaked at the 50th day of culture on sapwood for the Pa3 strain (17.618 mU/μg of protein) and at the 20th day of culture on heartwood for the Pa1 strain (12.203 mU/μg of protein). The activity of VP with manganese-oxidising properties was found to be high in all strains of P. abieticola, ranging 0–39.19 and 0–59.153 mU/μg of protein in sapwood and heartwood, respectively, whereas the activity of VP with guaiacol-oxidising properties was very low for all P. abieticola strains, ranging 0–0.248 and 0–0.225 mU/μg of protein in sapwood and heartwood, respectively. The values of released hydroxyphenols in P. abieticola strains ranged 24.915–139.766 and 25.19–84.562 µg of protocatechuic acid/ml in sapwood and heartwood, respectively. The values of released methoxyphenols for the evaluated strains of P. abieticola ranged 7.225–23.789 and 1.953–20.651 µg of vanillic acid/ml in sapwood and heartwood, respectively. Further studies with a higher number of strains of this species as well as an optimisation of conditions for the measurement of ligninolytic activity are needed.
Źródło:
Folia Forestalia Polonica. Series A . Forestry; 2019, 61, 4; 267-277
0071-6677
Pojawia się w:
Folia Forestalia Polonica. Series A . Forestry
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Intraspecific differentiation of reed grass Calamagrostis arundinacea [L.] Roth [Poaceae] populations revealed by peroxidase allozymes
Autorzy:
Krzakowa, M
Celka, Z.
Powiązania:
https://bibliotekanauki.pl/articles/58313.pdf
Data publikacji:
2008
Wydawca:
Polskie Towarzystwo Botaniczne
Tematy:
intraspecific differentiation
reed grass
Calamagrostis arundinacea
Poaceae
plant population
peroxidase allozyme
natural population
Polska
peroxidase
geographic region
Opis:
The genetic Variation of Reed Grass Calamagrostis arundinacea (L.) Roth was investigated in 25 populations in various geographic regions of Poland. A total of 907 individuals were sampled for electrophoretic analysis of peroxidase loci (11 allozymes). Populations were characterised by genetic parameters e.g. heterozygosity level, Wright's fixation index (F) and polymorphism coefficient (Pg). Mean values of interpopulation variability level (GST=0.0310), total genetic diversity (HT=0.4102) and gene flow between populations (Nm=7.805) were also examined. All the populations were polymorphic and they remain in Hardy Weinberg equilibrium.
Źródło:
Acta Societatis Botanicorum Poloniae; 2008, 77, 4; 299-304
0001-6977
2083-9480
Pojawia się w:
Acta Societatis Botanicorum Poloniae
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Kinetic studies on the oxidation of nitrite by horseradish peroxidase and lactoperoxidase
Autorzy:
Gębicka, Lidia
Powiązania:
https://bibliotekanauki.pl/articles/1044450.pdf
Data publikacji:
1999
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
lactoperoxidase
nitrite
stopped-flow
horseradish peroxidase
Opis:
The reaction of nitrite (NO-_2) with horseradish peroxidase and lactoperoxidase was studied. Sequential mixing sopped-flow measeruments gave the following values for the rate constants of the reaction of nitrite with compounds II (oxoferryl heme intermediates) of horseradish peroxidase and lactoperoxidase at pH 7.0, 13.3 ± 0.07 mol^{-1}dm^3s^{-1} and 3.5 ± 0.05 · 10^4mol^{-1}dm^3s^{-1}, respectively. Nitrite, at neutral pH, influenced measurements of activity of lactoperoxidase with typical substrates like 2,2'-azino-bis[ethyl-benzothiazoline-(6)-sulphonic acid] (ABTS), guaiacol or thiocyanate (SCN-). The rate of ABTS and guaiacol oxidation increased linearly with nitrite concentration up to 2.5-5 mmol dm^{-3}. On the other hand, two-electron SCN- oxidation was inhibited in the presence od nitrite. Thus, nitrite competed with the investigated substrates of lactoperoxidase. The intermediate, most probably nitrogen dioxide (*NO_2), reacted more rapidly with ABTS or guaiacol than did lactoperoxidase compound II. It did not, however, effectively oxidize SCN- to OSCN-. NO-_2 did not influence the activity measurements of horseradish peroxidase by ABTS or guaiacol method.
Źródło:
Acta Biochimica Polonica; 1999, 46, 4; 919-927
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Peroxidase and IAA oxidase activities during sink development in cotton seed.
Autorzy:
Rabadia, Vraj
Thaker, Vrinda
Singh, YeshDev
Powiązania:
https://bibliotekanauki.pl/articles/2199024.pdf
Data publikacji:
2006-06-22
Wydawca:
Instytut Hodowli i Aklimatyzacji Roślin
Tematy:
cotton
IAA oxidase
peroxidase
seed development
Opis:
Seeds of the three cotton genotypes, H-6 and H-4 (Gossypium hirsutum),and V797 (G. herbaceum) for their growth variations in IAA oxidase and peroxidase activities. The three genotypes varied in their final seed weight. The peroxidase analysis was done with four different hydrogen donors. Though the trends in peroxidase activitywere almost similar in the three genotypes, there was variation with different hydrogen donors. These was an inverse correlation between Cytoplasmic peroxidase and Wall bound peroxidase activity and rate of water uptake of the seed. The peroxidase activity showed up increasing levels only after the rate of water uptake decreased. However, IAA oxidase activity did not show any discernible trend with water content. The role of cytoplasmic and wall bound peroxidase in seed development is discussed in detail    
Źródło:
Plant Breeding and Seed Science; 2006, 53; 27-36
1429-3862
2083-599X
Pojawia się w:
Plant Breeding and Seed Science
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Controllable preparation of highly active horseradish peroxidase-gold nanoparticle bionanoconjugate
Autorzy:
Zhang, P.
Liu, Ch.
Song, S.
Peng, Ch.
Powiązania:
https://bibliotekanauki.pl/articles/778931.pdf
Data publikacji:
2012
Wydawca:
Zachodniopomorski Uniwersytet Technologiczny w Szczecinie. Wydawnictwo Uczelniane ZUT w Szczecinie
Tematy:
horseradish peroxidase
gold nanoparticles
bionanoconjugate
Opis:
A novel method of immobilizing horse radish peroxidase enzyme (HRP) onto the surface of gold nanoparticles (GNPs) was developed. As a result, a high-activity bionanoconjugates was obtained through utilizing the biotin-streptavidin (SA) system. The HRP-SA-GNP bionanoconjugate with high activity was conveniently prepared through the biotin- avidin system. Compared with the HRP-GNP bioconjugate prepared through the traditional electrostatic absorption method, the enzyme activity per GNPs of this new bionanoconjugate was enhanced by 10 times. Moreover, the enzyme activity of this bionanoconjugate was controllable. The above method of bionanoconjugation preparation has promising applications In the fi elds including preparing highly active bio-nanoprobe and immobilized enzyme.
Źródło:
Polish Journal of Chemical Technology; 2012, 14, 4; 57-60
1509-8117
1899-4741
Pojawia się w:
Polish Journal of Chemical Technology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Enzymatic assay of opioid analgesic, tramadol, using horseradish peroxidase
Autorzy:
Sridevi, N.
Srilakshmi, C.
Alekya, G.
Bala Sekaran, C.
Powiązania:
https://bibliotekanauki.pl/articles/10767.pdf
Data publikacji:
2014
Wydawca:
Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
Tematy:
opioid
tramadol
horseradish peroxidase
enzymatic method
Opis:
Three novel and sensitive enzymatic methods have been developed for the quantification of tramadol in pharmaceutical dosage forms. The proposed methods are based on the reaction of tramadol with 3-methylbenzothiazoline-2-one hydrazone (method A), aniline (method B) and aminoantipyrine in the presence of hydrogen peroxide and horseradish peroxidase to give colored complexes. The colored complexes obtained with 3-methylbenzothiazoline-2-one hydrazone, aniline and aminoantipyrine exhibit absorption maxima at 480 nm, 550 nm and 530 nm, respectively. Regression analysis of Beer’s plots showed good correlation for tramadol in the concentration range (μg/mL) 2-12 for the methods A & B and 4-24 for the method C. The experimental parameters were studied and optimized. The precision and accuracy of the methods were satisfactory. The proposed methods were successfully applied for the quantification of tramadol in 3 brands of commercially available tablet dosage forms. The results were compared satisfactorily with the official method.
Źródło:
International Letters of Natural Sciences; 2014, 11, 1
2300-9675
Pojawia się w:
International Letters of Natural Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The properties of peroxisomal ascorbate peroxidase from sugar beet (Beta vulgaris ) and expressed in E. coli
Autorzy:
Kurnik, K.
Skorupa-Klaput, M.
Tyburski, J.
Powiązania:
https://bibliotekanauki.pl/articles/80526.pdf
Data publikacji:
2013
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
conference
ascorbate peroxidase
sugar-beet
Beta vulgaris
Escherichia coli
expression
peroxidase activity
protein extract
BL21 cell
Źródło:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2013, 94, 3
0860-7796
Pojawia się w:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Bundle sheath-specific expression of the ascorbate peroxidase 2 affects photosynthesis in whole plant
Autorzy:
Gorecka, M.
Alvarez Fernandez, R.
Bialasek, M.
Mulleineaux, P.
Karpinski, S.
Powiązania:
https://bibliotekanauki.pl/articles/951327.pdf
Data publikacji:
2013
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
conference
sheath-specific expression
ascorbate peroxidase
hydrogen peroxide
photosynthesis
cytosolic peroxidase
fluorescence
electron transport chain
gene encoding
Źródło:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2013, 94, 2
0860-7796
Pojawia się w:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Deuterium isotope effects in oxidation of dopamine and norepinephrine catalyzed by horseradish peroxidase
Autorzy:
Byszewska, W.
Pająk, M.
Kańska, M.
Powiązania:
https://bibliotekanauki.pl/articles/146852.pdf
Data publikacji:
2013
Wydawca:
Instytut Chemii i Techniki Jądrowej
Tematy:
deuterium
dopamine (DA)
norepinephrine
isotope effects
oxidation
peroxidase
Opis:
Deuterium kinetic isotope effects (KIE’s) in the reaction of oxidation of dopamine (DA) catalyzed by the enzyme horseradish peroxidase (HRP, EC 1.11.1.7) was determined using a non-competitive spectrophotometric method. For kinetic studies, the deuterium ring labelled isotopomer [2’,5’,6’-2H3]-dopamine was synthesized using acid catalyzed isotopic exchange between authentic dopamine and heavy water. Deuterium solvent isotope effects (SIE’s) for dopamine and norepinephrine were determined separately for the enzymatic oxidation carried out in the presence or absence of hydrogen peroxide. Some mechanistic details of enzymatic oxidation of dopamine and norepinephrine to corresponding catecholchromes catalyzed by HRP were discussed.
Źródło:
Nukleonika; 2013, 58, 4; 475-479
0029-5922
1508-5791
Pojawia się w:
Nukleonika
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Effect of temperature on guaiacol peroxidase of Pyrus communis
Autorzy:
Saeidian, S.
Ghasemifar, E.
Powiązania:
https://bibliotekanauki.pl/articles/10793.pdf
Data publikacji:
2013
Wydawca:
Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
Tematy:
temperature effect
guaiacol
Peroxidase
Pyrus communis
enzyme
plant growth
plant development
cell wall
Opis:
Peroxidase (EC 1.11.1.7; donor: hydrogen-peroxide oxidoreductase, POD) is one of the key enzymes controlling plant growth, differentiation and development. The enzyme participates in construction, rigidification and eventual lignification of cell walls, biosynthesis of ethylene from 1-aminocyclopropane-1-carboxylic acid and H2O2, regulation of auxin level through auxin catabolism, protection of tissue from damage and infection by pathogenic microorganisms, the oxidation of indoleacetic acid. For peroxidase activity in wild pears extract one pH optimum was observed at 6.5 that probably belong to atleast one isoenzyme. Activity of peroxidase in presence of guaiacol and H2O2 was optimum after incubation at 40 °C. Maximum activity of peroxidase is 300 % .Activity increased to 240 %, 300 %, 70 % and 10 % after 60 minute incubation at 30, 40, 45 and 60 °C for peroxidase. Incubation at high temperature (70 °C) was accompanied with decrease of activity to 10 % peroxidase activity.
Źródło:
International Letters of Natural Sciences; 2013, 05
2300-9675
Pojawia się w:
International Letters of Natural Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Cadmium-induced changes in antioxidant enzymes in suspension culture of soybean cells.
Autorzy:
Sobkowiak, Robert
Rymer, Katarzyna
Rucińska, Renata
Deckert, Joanna
Powiązania:
https://bibliotekanauki.pl/articles/1043349.pdf
Data publikacji:
2004
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
ascorbate peroxidase
superoxide dismutase
peroxidase
soybean cell suspension culture
catalase
cadmium
Opis:
Cadmium (Cd), similarly to other heavy metals, inhibits plant growth. We have recently showed that Cd2+ either stimulates (1-4 μM) or inhibits (ł 6 μM) growth of soybean (Glycine max L.) cells in suspension culture (Sobkowiak & Deckert, 2003, Plant Physiol Biochem. 41: 767-72). Here, soybean cell suspension cultures were treated with various concentrations of Cd2+ (1-10 μM) and the following enzymes were analyzed by native electrophoresis: superoxide dismutase (SOD), catalase (CAT), peroxidase (POX) and ascorbate peroxidase (APOX). We found a significant correlation between the cadmium-induced changes of soybean cell culture growth and the isoenzyme pattern of the antioxidant enzymes. The results suggest that inhibition of growth and modification of antioxidant defense reactions appear in soybean cells when Cd2+ concentration in culture medium increases only slightly, from 4 to 6 μM.
Źródło:
Acta Biochimica Polonica; 2004, 51, 1; 219-222
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Effect of nanosilver on physiological performance of pelargonium plants exposed to dark storage
Autorzy:
Hatami, M.
Ghorbanpour, M.
Powiązania:
https://bibliotekanauki.pl/articles/1826.pdf
Data publikacji:
2013
Wydawca:
Instytut Ogrodnictwa
Tematy:
Pelargonium
Pelargonium x hortorum
nanosilver
leaf senescence
storage
antioxidative enzyme
lipid peroxidation
ascorbate peroxidase
guaiacol peroxidase
Źródło:
Journal of Horticultural Research; 2013, 21, 1
2300-5009
Pojawia się w:
Journal of Horticultural Research
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Blood leukocytes and eosinophil peroxidase are not related to faecal egg counts in Polish Wrzosowka hoggets naturally infected with gastrointestinal nematodes
Autorzy:
Moskwa, B.
Doligalska, M.
Cabaj, W.
Powiązania:
https://bibliotekanauki.pl/articles/839118.pdf
Data publikacji:
1998
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
parasite
gastrointestinal nematode
blood
nematode
leucocyte
eosinophil peroxidase
Źródło:
Annals of Parasitology; 1998, 44, 3
0043-5163
Pojawia się w:
Annals of Parasitology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Effect of copper(II) the activity of glutathione peroxidase in patients with head and neck cancer
Autorzy:
Malinowska, Katarzyna
Morawiec-Sztandera, Alina
Majsterek, Ireneusz
Kaczmarczyk, Dariusz
Powiązania:
https://bibliotekanauki.pl/articles/1398625.pdf
Data publikacji:
2016
Wydawca:
Index Copernicus International
Tematy:
pyrazole complex
glutathione peroxidase
head and neck cancer
Opis:
Introduction: Head and neck squamous cell carcinoma (HNSCC) accounts for about 6% of all malignant cancers. In the epidemiology of oral cavity neoplasm, important risk factors include: tobacco smoking, alcohol abuse, bad oral hygiene, papilloma virus infection, riboflavin and iron deficiency. Objective: The objective of the investigation was a synthesis of Cu(II) complex and the evaluation of antioxidative enzymatic barrier in red blood cells of patients with head and neck tumor as well as in the control group. Materials and methods: For the investigation conduction, a consent of Bioethics Committee number RNN/142/09/KB was obtained. Blood for the examination was obtained from the patients of the Dapartment of Head and Neck Neoplasms Surgery Medical University of Łódź. The experiment was conducted on the group of 40 patients with HNSCC and 40 healthy people, using spectrophotometric method, glutathione peroxidase was marked. Results: The investigation was conducted on the hemolysate obtained from the patients that were divided into two groups – a study group (1 and 2), which consisted of patients diagnosed with head and neck cancer and a control group (1 and 2) – healthy people. A significant statistical result for GPX occurred in control-1 and study-1 group with complex compound Cu(II) (p<0,001). Conclusions: Presented research prove, that complex compound Cis-dichlorobis(N1-hydroxymethyl-3methylpyrazole-κN2)copper (II) has an impact on the activity of the antioxidative GPX enzyme.
Źródło:
Polish Journal of Otolaryngology; 2016, 70, 6; 20-25
0030-6657
2300-8423
Pojawia się w:
Polish Journal of Otolaryngology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Grain discoloration in different genotypes of durum wheat (Triticum durum L.) in Argentina: associated mycobiota and peroxidase activity
Autorzy:
Cipollone, M.J.
Moya, P.
Martinez, I.
Saparrat, M.
Sisterna, M.
Powiązania:
https://bibliotekanauki.pl/articles/2084746.pdf
Data publikacji:
2020
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
durum wheat
grain discoloration
mycobiota
peroxidase activity
Opis:
Wheat grain discoloration, a worldwide disease that lowers grain quality and decreases grain yield, does not have a single etiology. It has been proposed that it is a consequence of an abiotic mechanism, a response to environmental conditions or enzymatic activity. It has also been suggest that it is a biotic mechanism, a fungal infection principally by Alternaria spp. and Bipolaris sorokiniana. The present work was carried out to analyze the possible etiology of this disease in nine durum wheat genotypes from two localities of southern Buenos Aires province (Argentina) on two sowing dates. Incidence (percentage of grain discoloration) was recorded and mycobiota associated with this pathology was registered following ISTA rules. Peroxidase activity in an extract obtained from grains belonging to genotypes of the locality that showed the highest incidence was measured. The incidence among genotypes, localities and sowing dates varied, although the genotypes with the higher and lower values of incidence were the same for all the variables tested. The fungus Alternaria spp. was isolated the most frequently followed by Fusarium spp., while Bipolaris sorokiniana was found the least frequently. Peroxidase activity showed that all the treatments had similar levels of enzymatic activity, but there was no clear differentiation between controls either between genotypes with the lowest or the highest incidence values. This suggests that peroxidase activity did not have a clear relationship with grain discoloration. In this research, it is presumed that fungal infection is the main cause of this disease.
Źródło:
Journal of Plant Protection Research; 2020, 60, 1; 14-20
1427-4345
Pojawia się w:
Journal of Plant Protection Research
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Red blood cell and plasma glutathione peroxidase activities and selenium concentration in patients with chronic kidney disease: A review
Autorzy:
Zachara, Bronisław
Gromadzińska, Jolanta
Wąsowicz, Wojciech
Zbróg, Zbigniew
Powiązania:
https://bibliotekanauki.pl/articles/1041155.pdf
Data publikacji:
2006
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
hemodialysis
selenium
kidney transplantation
glutathione peroxidase
chronic kidney disease
antioxidants
plasma
Opis:
The metabolism of oxygen in aerobic organisms leads to generation of reactive oxygen species (ROS). These entities are able to oxidize almost all classes of macromolecules, including proteins, lipids and nucleic acids. The physiological level of ROS is usually regulated by antioxidant defense mechanisms. There are at least three groups of antioxidant enzymes: superoxide dismutases, catalases and glutathione peroxidases (GSH-Pxs) which neutralize ROS. The trace elements (copper, zinc and selenium) bound to the active sites of the above listed enzymes play an important role in the antioxidant defense system. In mammals, a major function of selenium (Se) and Se-dependent GSH-Pxs is to protect cells from oxidative stress. Selenium concentrations and GSH-Px activities are altered in blood components of chronic kidney disease (CKD) patients. The Se level is frequently lower than in healthy subjects and the concentration very often decreases gradually with advancing stage of the disease. Studies on red cell GSH-Px activity in CKD patients reported its values significantly lower, significantly higher and lower or higher, but not significantly as compared with healthy subjects. On the other hand, all authors who studied plasma GSH-Px activity have shown significantly lower values than in healthy subjects. The degree of the reduction decreases gradually with the progression of the disease. High inverse correlations were seen between plasma GSH-Px activity and creatinine level. A gradual decrease in plasma GSH-Px activity in CKD patients is due to the fact that this enzyme is synthesized predominantly in the kidney and thus the impairment of this organ is the cause of the enzyme's lower activity. Se supplementation to CKD patients has a slightly positive effect in the incipient stage of the disease, but usually no effect was observed in end-stage CKD. Presently, kidney transplantation is the only treatment that may restore plasma Se level and GSH-Px activity in patients suffering from end-stage CKD. A few studies have shown that in kidney recipients, plasma Se concentration and GSH-Px activity are restored to normal values within a period of 2 weeks to 3 months following surgery and thus it can be acknowledged that Se supplementation to those patients has a positive effect on plasma GSH-Px activity.
Źródło:
Acta Biochimica Polonica; 2006, 53, 4; 663-677
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Differential response of antioxidant enzymes to cadmium stress in tolerant and sensitive cell line of cucumber (Cucumis sativus L.)
Autorzy:
Gzyl, Jarosław
Rymer, Katarzyna
Gwóźdź, Edward
Powiązania:
https://bibliotekanauki.pl/articles/1040497.pdf
Data publikacji:
2009
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
cell suspension
cadmium
tolerance
superoxide dismutase
catalase
ascorbate peroxidase
guaiacol peroxidase
Opis:
Previously, a stable cell suspension of cucumber tolerant to 100 µM CdCl2 was obtained (Gzyl & Gwóźdź, 2005, Plant Cell Tissue Organ Cult 80: 59-67). In this study, the relationship between the activity of antioxidant enzymes and cadmium tolerance of cucumber cells was analyzed. A cadmium-sensitive and the cadmium-tolerant cell lines were exposed to 100 µM and 200 µM CdCl2 and the activities of superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APOX) and guaiacol peroxidase (POX) were determined. In the sensitive cell line, a decrease of total activity of SOD and POX was observed, whereas the activity of CAT and APOX significantly increased in metal-supplemented medium. By contrast, in the tolerant cells, the total activity of antioxidant enzymes decreased (SOD, CAT) or was maintained at approximately the same level (APOX, POX). Moreover, a different pattern of isoenzyme activity was observed in the tolerant and sensitive cells. These results suggest that an enhanced activity of antioxidant enzymes is not directly involved in the increased tolerance to cadmium of the selected cucumber cell line.
Źródło:
Acta Biochimica Polonica; 2009, 56, 4; 723-727
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Porównanie oddziaływania selenu na aktywność peroksydazową gleby skażonej olejem napędowym lub przepracowanym olejem silnikowym
Comparison of selenium effect on peroxidase activities in soil contaminated with diesel oil and spent engine oil
Autorzy:
Strek, M.
Telesinski, A.
Powiązania:
https://bibliotekanauki.pl/articles/36028.pdf
Data publikacji:
2016
Wydawca:
Polska Akademia Nauk. Instytut Agrofizyki PAN
Tematy:
gleby
selen
zanieczyszczenia gleb
olej napedowy
olej silnikowy
olej silnikowy przepracowany
peroksydaza
aktywnosc peroksydazowa
soil
selenium
soil contaminant
diesel fuel
engine oil
peroxidase
peroxidase activity
Opis:
W pracy przedstawiono wyniki oddziaływania oleju napędowego, przepracowanego oleju silnikowego oraz selenu (IV i VI), na aktywność peroksydazową gleby. Doświadczenie przeprowadzono w warunkach laboratoryjnych na piasku gliniastym o zawartości węgla organicznego 8,7 g·kgˉ¹. Do próbek gleby wprowadzono w różnych kombinacjach kwas selenowy (IV) lub kwas selenowy (VI) (ilość dodanego Se wynosiła 0,05 mmol·kgˉ¹) oraz przepracowany olej silnikowy lub olej napędowy w ilościach 2, 10 i 50 g·kgˉ¹. Wszystkie próbki doprowadzono do 60% maksymalnej pojemności wodnej i przechowywano w szklanych pojemnikach typu twist, w stałej temperaturze 20°C. Aktywność peroksydaz oznaczono spektrofotometrycznie w 1., 7., 14., 28., 56. i 112. dniu doświadczenia. Skażenie gleby olejem napędowym oraz przepracowanym olejem silnikowym spowodowało stymulację aktywności peroksydaz. Wprowadzenie selenu do gleby nieskażonej substancjami ropopochodnymi wywołało zmiany aktywności peroksydaz. Jedynie dodatek selenu VI do gleby zawierającej olej napędowy w dawce 2 g·kgˉ¹ spowodował istotny wzrost średniej aktywności peroksydaz glebowych.
This paper describes the impact of diesel oil, spent engine oil and selenium (IV and VI) on peroxidase activity in soil. Experiment was carried out in laboratory conditions on loamy sand with organic carbon content of 8.7 g·kgˉ¹. Different combinations of selenic (IV) acid or selenic (VI) acid (the Se amount was 0.05 mmol·kgˉ¹) and diesel oil or spent engine oil at dosage of 2, 10 i 50 g·kgˉ¹ were added to soil samples. All samples were adjusted to 60% of the maximum water holding capacity and stored in glass twist containers at a temperature of 20°C. Activity of peroxidases was determined spectrophotometrically on days 1, 7, 14, 28, 56 and 112. Soil contamination with diesel oil and spent engine oil increased the activity of peroxidases. Application of selenium to soil uncontaminated with petroleum hydrocarbons caused significant changes of peroxidase activities in the initial period of experiment. Only in soil containing diesel oil at the dosage of 2 g·kgˉ¹ significant increase in activity of peroxidase was observed after treatment with selenium VI.
Źródło:
Acta Agrophysica; 2016, 23, 3
1234-4125
Pojawia się w:
Acta Agrophysica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Właściwości fizykochemiczne produktów enzymatycznego utleniania skrobi
Physicochemical properties of products of enzymatic oxidation of starch
Autorzy:
Roczkowska, M.
Siemion, P.
Kaczmarek, A.
Powiązania:
https://bibliotekanauki.pl/articles/410267.pdf
Data publikacji:
2013
Wydawca:
Uniwersytet Humanistyczno-Przyrodniczy im. Jana Długosza w Częstochowie. Wydawnictwo Uczelniane
Tematy:
skrobia
skrobia utleniona
utlenianie enzymatyczne
peroksydaza ligninowa
LiP
peroksydaza manganozależna
MnP
starch
oxidized starch
enzymatic oxidation
lignin peroxidase
manganese peroxidase
Opis:
Reakcje enzymatyczne coraz częściej uzupełniają i zastępują wszelkie reakcje chemiczne. Są wysoce selektywne i precyzyjne, gdyż enzymy działają na konkretne grupy czy wiązania chemiczne. Przykładem enzymów wykorzystywanych w procesach utleniania są lakazy oraz peroksydazy – ligninowa (LiP) i manganozależna (MnP). Niniejsza praca została poświęcona badaniom właściwości fizykochemicznych skrobi utlenionych enzymatycznie przy użyciu peroksydazy ligninowej (LiP) oraz manganozależnej (MnP). Badaniom zostały poddane utlenione skrobie kukurydziane oraz ziemniaczane. Badania wykazały, że we wszystkich analizowanych próbkach doszło do utlenienia enzymatycznego. Produkty modyfikacji odznaczały się odmiennymi właściwościami fizykochemicznymi w stosunku do próbek natywnych skrobi tego samego gatunku. Zmianie uległa zdolność pochłaniania wody, rozpuszczalność w wodzie i trwałość w wysokich temperaturach.
The enzymatic reactions increasingly replace and complement any chemical reactions. They are highly selective and precise, because the enzymes act on specific groups or chemical bonds. The examples of enzymes applied in the oxidation processes are laccases, lignin (LiP) and manganese (MnP) peroxidases. This work has been devoted to the studies on physicochemical properties of the enzymatically oxidized starches using lignin (LiP) and manganese (MnP) peroxidases. The studies were performed on oxidized corn and potato starches. The studies have shown that the enzymatic oxidation had occurred in all of the analyzed samples. The products of modification showed different physicochemical properties in comparison to native starch samples of the same species. The water absorption capacity, water solubility and stability at high temperatures have been altered.
Źródło:
Chemistry, Environment, Biotechnology; 2013, 16; 85-96
2083-7097
Pojawia się w:
Chemistry, Environment, Biotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Peroxidase activity and soluble carbohydrates content in pea roots in response to soil contamination with oxytetracycline
Autorzy:
Dobiesz, M.
Ziolkowska, A.
Piotrowicz-Cieslak, A.I.
Powiązania:
https://bibliotekanauki.pl/articles/80780.pdf
Data publikacji:
2013
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
conference
peroxidase activity
carbohydrate content
pea
soil contamination
oxytetracycline
Streptomyces rimosus
bacteriostatic activity
antioxidative enzyme
Źródło:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2013, 94, 3
0860-7796
Pojawia się w:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Can changes in starch content and peroxidase activity be used as rooting phase markers for rhododendron leaf bud cuttings?
Autorzy:
Megre, D.
Dokane, K.
Kondratovics, U.
Powiązania:
https://bibliotekanauki.pl/articles/19397.pdf
Data publikacji:
2011
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
content change
starch content
peroxidase activity
rooting phase marker
rhododendron
leaf bud cutting
propagation
rooting
Opis:
We examined whether peroxidase activity in cutting bases and leaves and starch content in cutting bases can be used as rooting phase markers in the elepidote rhododendron cv. 'Babites Baltais' (Rhododendron L.). Changes in peroxidase activity in cutting leaves and bases, as well as starch content in cutting bases, were determined in relation to anatomical stages of rhizogenesis in leaf bud cuttings treated with 1% indole-3-butyric acid (IBA+) or without IBA (IBA–). The pattern of change of peroxidase activity was similar in cutting bases and leaves of IBAleaf bud cuttings. Three phases of adventitious root formation were identified: induction, initiation and expression. During the induction phase peroxidase activity decreased, but no anatomical changes were observed in the cuttings. Peroxidase activity increased in the initiation phase when adventitious root initials were formed. Peroxidase activity decreased during the expression phase when adventitious root primordia developed. The starch content of IBA– leaf bud cuttings decreased during the first few days and then gradually rose to maximum, followed by a sharp reduction and another increase at the end of the experiment. The changes of starch content did not coincide with rooting phases as peroxidase activity did, and cannot be used as a rooting phase marker in rhododendrons. Adventitious root formation did not occur in IBA+ leaf bud cuttings, so distinct rooting phases could not be observed. There was a significant correlation between peroxidase activity in cutting bases and leaves of IBA- leaf bud cuttings. Peroxidase activity in leaves of rhododendron leaf bud cuttings are potentially useful as a marker for rooting phases, but that requires further anatomical and physiological study of rooting in leaf bud cuttings.
Źródło:
Acta Biologica Cracoviensia. Series Botanica; 2011, 53, 1
0001-5296
Pojawia się w:
Acta Biologica Cracoviensia. Series Botanica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The effect of methylprednisolone on the dynamics of oxygen uptake and peroxidase and superoxide dismutase activities in skeletal muscles of mice infected with Trichinella spiralis larvae
Autorzy:
Wandurska-Nowak, E.
Boczon, K.
Derda, M.
Powiązania:
https://bibliotekanauki.pl/articles/838559.pdf
Data publikacji:
1998
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
parasite
superoxide dismutase
mouse
mice
larva
peroxidase
Trichinella spiralis
skeletal muscle
Źródło:
Annals of Parasitology; 1998, 44, 3
0043-5163
Pojawia się w:
Annals of Parasitology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Superoxide dismutase and glutathione peroxidase activity in pregnancy complicated by diabetes
Autorzy:
Wdowiak, A.
Brzozowski, I.
Bojar, I.
Powiązania:
https://bibliotekanauki.pl/articles/50976.pdf
Data publikacji:
2015
Wydawca:
Instytut Medycyny Wsi
Źródło:
Annals of Agricultural and Environmental Medicine; 2015, 22, 2
1232-1966
Pojawia się w:
Annals of Agricultural and Environmental Medicine
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Selenium supplementation to chronic kidney disease patients on hemodialysis does not induce the synthesis of plasma glutathione peroxidase
Autorzy:
Zachara, Bronislaw
Gromadzinska, Jolanta
Zbrog, Zbigniew
Swiech, Rafal
Wasowicz, Wojciech
Twardowska, Ewa
Jablonska, Ewa
Sobala, Wojciech
Powiązania:
https://bibliotekanauki.pl/articles/1040656.pdf
Data publikacji:
2009
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
hemodialysis
selenium supplementation
glutathione peroxidase
chronic kidney disease
plasma
Opis:
Background: Numerous authors have shown that selenium (Se) concentration and glutathione peroxidase (GSH-Px) activity in plasma of chronic kidney disease (CKD) patients are lower than in healthy subjects, but there are only few publications on the level of GSH-Px protein in those patients and no reports on the effect of Se supplementation to HD patients on the level of this enzyme. Subjects and Methods: Se concentration and GSH-Px protein level in plasma were measured in a group of 30 CKD patients on hemodialysis (HD) supplemented with 200 µg Se/day for 3 months, and 28 patients on HD administered with placebo. Se concentration was measured by graphite furnace atomic absorption spectrometry and plasma GSH-Px protein level by the sandwich ELISA method using polyclonal antibody specific for human plasma GSH-Px. Results: Se concentration in patients on placebo did not change throughout the 3-month study period, but increased significantly in Se supplemented group. Se supplementation to CKD patients on HD had no effect on the level of GSH-Px protein. Conclusions: The lack of GSH-Px protein in CKD patients on HD is not linked to Se deficiency since the level of this element increased after Se supplementation while enzyme protein level did not change. The damaged kidney of HD patients is unable to synthesize GSH-Px, even after induction with selenium.
Źródło:
Acta Biochimica Polonica; 2009, 56, 1; 183-187
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Ligninolityczne właściwości izolatów Heterobasidion parviporum w hodowli na drewnie świerka
Ligninolytic activity of Heterobasidion parviporum isolates in cultivation on Norway spruce wood
Autorzy:
Żółciak, A.
Bohacz, J.
Powiązania:
https://bibliotekanauki.pl/articles/989467.pdf
Data publikacji:
2016
Wydawca:
Polskie Towarzystwo Leśne
Tematy:
lesnictwo
fitopatologia lesna
grzyby
Heterobasidion parviporum
izolaty grzybowe
aktywnosc lignolityczna
enzymy lignolityczne
lakaza
peroksydaza manganozalezna
peroksydaza ligninowa
peroksydaza "versatile"
rozklad drewna
drewno bielu
drewno twardzieli
drewno swierkowe
biodegradacja
laccase
manganese peroxidase
lignin peroxidase
versatile peroxidase
hydroxy−
methoxyphenols
sapwood
heartwood
norway spruce wood
Opis:
The aim of this preliminary study was to assess the activity of laccase, diverse peroxidases as well as the level of micromolecular compounds in Heterobasidion parviporum isolates grown on pieces of Norway spruce wood (sapwood and heartwood) during 50 days of incubation under the laboratory conditions. H. parviporum isolates secreted extracellular enzymes: laccase, manganese peroxidase (MnP), lignin peroxidase (LiP) and versatile peroxidase (VP). Hydroxy− and methoxyphenols were also released during this process. The above−mentioned enzymes showed low activity in mycelium grown on both sapwood and heartwood. The activity of laccase ranged from 0 to 0.513 mU/mg protein on sapwood, and from 0 to 0.106 mU/mg protein – on heartwood. MnP activity of H. parviporum isolates ranged from 0.024 to 0.667 mU/mg protein on sapwood, and from 0.038 to 1.585 mU/mg protein – on heartwood. LiP activity was small and ranged from 0 to 1.281 mU/mg protein on sapwood, and from 0.013 to 0.166 mU/mg protein – on heartwood. Activity of VP oxidizing manganese ions was low. It ranged from 0 to 3.063 mU/mg protein on sapwood, and from 0.059 to 3.054 mU/mg protein – on heartwood. The activity of VP oxidizing guaiacol ranged from 0.006 to 1.490 mU/mg protein on sapwood and from 0.038 to 1.147 mU/mg protein – on heartwood. The hydroxyphenols produced by H. parviporum isolates ranged from 15.037 to 110.149 mg of protocatechuic acid/ml on sapwood, and from 11.236 to 27.220 mg of protocatechuic acid/ml – on heartwood. Methoxyphenols produced by H. parviporum isolates ranged from 3.393 to 24.253 mg of vanillic acid/ml on sapwood, and from 4.955 to 12.005 mg of vanillic acid/ml – on heartwood.
Źródło:
Sylwan; 2016, 160, 12; 1027-1035
0039-7660
Pojawia się w:
Sylwan
Dostawca treści:
Biblioteka Nauki
Artykuł
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