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Tytuł:
Determination of Pleurotus abieticola ligninolytic activity on norway spruce wood
Autorzy:
Żółciak, Anna
Powiązania:
https://bibliotekanauki.pl/articles/2041965.pdf
Data publikacji:
2019
Wydawca:
Instytut Badawczy Leśnictwa
Tematy:
laccase
lignin peroxidase
manganese peroxidase
versatile peroxidase
spruce
white rot fungus
wood
Opis:
The effect of Phlebiopsis gigantea treatment in control of Heterobasidion parviporum in Norway spruce is less effective than that in control of Heterobasidion annosum in pine. It is necessary to apply other fungi, for example, Pleurotus abieticola in Norway spruce stands. Thus, it is necessary to assess the activity of major ligninolytic enzymes, that is, laccase, lignin peroxidase (LiP), manganese peroxidase (MnP) and versatile peroxidase (VP) produced by P. abieticola, which may be effective in the fast degradation of Norway spruce wood. Three strains of P. abieticola (Pa1-3) were grown on pieces of Norway spruce sapwood and heartwood for 50 days in laboratory conditions. Enzymatic activity was determined using spectrophotometry. Pleurotus abieticola produced laccase, LiP, MnP and VP. The activity of laccase was low, ranging 0–3.696 and 0–0.806 mU/μg of protein in sapwood and heartwood, respectively. The highest activity in Pa1 = 3.696 mU/μg of protein in sapwood and in Pa3 = 0.806 mU/μg of protein in heartwood was observed after 30 and 50 days of incubation, respectively. The activity of LiP was also low, ranging 0–0.188 and 0–0.271 mU/μg of protein in sapwood and heartwood, respectively. The highest activity in Pa1 = 0.271 mU/μg of protein in sapwood and in Pa2 = 0.188 mU/μg of protein in heartwood was observed after 40 and 20 days of incubation, respectively. The activity of MnP ranged 0–17.618 and 0–12.203 mU/μg of protein in sapwood and heartwood, respectively. This enzymatic activity peaked at the 50th day of culture on sapwood for the Pa3 strain (17.618 mU/μg of protein) and at the 20th day of culture on heartwood for the Pa1 strain (12.203 mU/μg of protein). The activity of VP with manganese-oxidising properties was found to be high in all strains of P. abieticola, ranging 0–39.19 and 0–59.153 mU/μg of protein in sapwood and heartwood, respectively, whereas the activity of VP with guaiacol-oxidising properties was very low for all P. abieticola strains, ranging 0–0.248 and 0–0.225 mU/μg of protein in sapwood and heartwood, respectively. The values of released hydroxyphenols in P. abieticola strains ranged 24.915–139.766 and 25.19–84.562 µg of protocatechuic acid/ml in sapwood and heartwood, respectively. The values of released methoxyphenols for the evaluated strains of P. abieticola ranged 7.225–23.789 and 1.953–20.651 µg of vanillic acid/ml in sapwood and heartwood, respectively. Further studies with a higher number of strains of this species as well as an optimisation of conditions for the measurement of ligninolytic activity are needed.
Źródło:
Folia Forestalia Polonica. Series A . Forestry; 2019, 61, 4; 267-277
0071-6677
Pojawia się w:
Folia Forestalia Polonica. Series A . Forestry
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Intraspecific differentiation of reed grass Calamagrostis arundinacea [L.] Roth [Poaceae] populations revealed by peroxidase allozymes
Autorzy:
Krzakowa, M
Celka, Z.
Powiązania:
https://bibliotekanauki.pl/articles/58313.pdf
Data publikacji:
2008
Wydawca:
Polskie Towarzystwo Botaniczne
Tematy:
intraspecific differentiation
reed grass
Calamagrostis arundinacea
Poaceae
plant population
peroxidase allozyme
natural population
Polska
peroxidase
geographic region
Opis:
The genetic Variation of Reed Grass Calamagrostis arundinacea (L.) Roth was investigated in 25 populations in various geographic regions of Poland. A total of 907 individuals were sampled for electrophoretic analysis of peroxidase loci (11 allozymes). Populations were characterised by genetic parameters e.g. heterozygosity level, Wright's fixation index (F) and polymorphism coefficient (Pg). Mean values of interpopulation variability level (GST=0.0310), total genetic diversity (HT=0.4102) and gene flow between populations (Nm=7.805) were also examined. All the populations were polymorphic and they remain in Hardy Weinberg equilibrium.
Źródło:
Acta Societatis Botanicorum Poloniae; 2008, 77, 4; 299-304
0001-6977
2083-9480
Pojawia się w:
Acta Societatis Botanicorum Poloniae
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Kinetic studies on the oxidation of nitrite by horseradish peroxidase and lactoperoxidase
Autorzy:
Gębicka, Lidia
Powiązania:
https://bibliotekanauki.pl/articles/1044450.pdf
Data publikacji:
1999
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
lactoperoxidase
nitrite
stopped-flow
horseradish peroxidase
Opis:
The reaction of nitrite (NO-_2) with horseradish peroxidase and lactoperoxidase was studied. Sequential mixing sopped-flow measeruments gave the following values for the rate constants of the reaction of nitrite with compounds II (oxoferryl heme intermediates) of horseradish peroxidase and lactoperoxidase at pH 7.0, 13.3 ± 0.07 mol^{-1}dm^3s^{-1} and 3.5 ± 0.05 · 10^4mol^{-1}dm^3s^{-1}, respectively. Nitrite, at neutral pH, influenced measurements of activity of lactoperoxidase with typical substrates like 2,2'-azino-bis[ethyl-benzothiazoline-(6)-sulphonic acid] (ABTS), guaiacol or thiocyanate (SCN-). The rate of ABTS and guaiacol oxidation increased linearly with nitrite concentration up to 2.5-5 mmol dm^{-3}. On the other hand, two-electron SCN- oxidation was inhibited in the presence od nitrite. Thus, nitrite competed with the investigated substrates of lactoperoxidase. The intermediate, most probably nitrogen dioxide (*NO_2), reacted more rapidly with ABTS or guaiacol than did lactoperoxidase compound II. It did not, however, effectively oxidize SCN- to OSCN-. NO-_2 did not influence the activity measurements of horseradish peroxidase by ABTS or guaiacol method.
Źródło:
Acta Biochimica Polonica; 1999, 46, 4; 919-927
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Peroxidase and IAA oxidase activities during sink development in cotton seed.
Autorzy:
Rabadia, Vraj
Thaker, Vrinda
Singh, YeshDev
Powiązania:
https://bibliotekanauki.pl/articles/2199024.pdf
Data publikacji:
2006-06-22
Wydawca:
Instytut Hodowli i Aklimatyzacji Roślin
Tematy:
cotton
IAA oxidase
peroxidase
seed development
Opis:
Seeds of the three cotton genotypes, H-6 and H-4 (Gossypium hirsutum),and V797 (G. herbaceum) for their growth variations in IAA oxidase and peroxidase activities. The three genotypes varied in their final seed weight. The peroxidase analysis was done with four different hydrogen donors. Though the trends in peroxidase activitywere almost similar in the three genotypes, there was variation with different hydrogen donors. These was an inverse correlation between Cytoplasmic peroxidase and Wall bound peroxidase activity and rate of water uptake of the seed. The peroxidase activity showed up increasing levels only after the rate of water uptake decreased. However, IAA oxidase activity did not show any discernible trend with water content. The role of cytoplasmic and wall bound peroxidase in seed development is discussed in detail    
Źródło:
Plant Breeding and Seed Science; 2006, 53; 27-36
1429-3862
2083-599X
Pojawia się w:
Plant Breeding and Seed Science
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Controllable preparation of highly active horseradish peroxidase-gold nanoparticle bionanoconjugate
Autorzy:
Zhang, P.
Liu, Ch.
Song, S.
Peng, Ch.
Powiązania:
https://bibliotekanauki.pl/articles/778931.pdf
Data publikacji:
2012
Wydawca:
Zachodniopomorski Uniwersytet Technologiczny w Szczecinie. Wydawnictwo Uczelniane ZUT w Szczecinie
Tematy:
horseradish peroxidase
gold nanoparticles
bionanoconjugate
Opis:
A novel method of immobilizing horse radish peroxidase enzyme (HRP) onto the surface of gold nanoparticles (GNPs) was developed. As a result, a high-activity bionanoconjugates was obtained through utilizing the biotin-streptavidin (SA) system. The HRP-SA-GNP bionanoconjugate with high activity was conveniently prepared through the biotin- avidin system. Compared with the HRP-GNP bioconjugate prepared through the traditional electrostatic absorption method, the enzyme activity per GNPs of this new bionanoconjugate was enhanced by 10 times. Moreover, the enzyme activity of this bionanoconjugate was controllable. The above method of bionanoconjugation preparation has promising applications In the fi elds including preparing highly active bio-nanoprobe and immobilized enzyme.
Źródło:
Polish Journal of Chemical Technology; 2012, 14, 4; 57-60
1509-8117
1899-4741
Pojawia się w:
Polish Journal of Chemical Technology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Enzymatic assay of opioid analgesic, tramadol, using horseradish peroxidase
Autorzy:
Sridevi, N.
Srilakshmi, C.
Alekya, G.
Bala Sekaran, C.
Powiązania:
https://bibliotekanauki.pl/articles/10767.pdf
Data publikacji:
2014
Wydawca:
Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
Tematy:
opioid
tramadol
horseradish peroxidase
enzymatic method
Opis:
Three novel and sensitive enzymatic methods have been developed for the quantification of tramadol in pharmaceutical dosage forms. The proposed methods are based on the reaction of tramadol with 3-methylbenzothiazoline-2-one hydrazone (method A), aniline (method B) and aminoantipyrine in the presence of hydrogen peroxide and horseradish peroxidase to give colored complexes. The colored complexes obtained with 3-methylbenzothiazoline-2-one hydrazone, aniline and aminoantipyrine exhibit absorption maxima at 480 nm, 550 nm and 530 nm, respectively. Regression analysis of Beer’s plots showed good correlation for tramadol in the concentration range (μg/mL) 2-12 for the methods A & B and 4-24 for the method C. The experimental parameters were studied and optimized. The precision and accuracy of the methods were satisfactory. The proposed methods were successfully applied for the quantification of tramadol in 3 brands of commercially available tablet dosage forms. The results were compared satisfactorily with the official method.
Źródło:
International Letters of Natural Sciences; 2014, 11, 1
2300-9675
Pojawia się w:
International Letters of Natural Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The properties of peroxisomal ascorbate peroxidase from sugar beet (Beta vulgaris ) and expressed in E. coli
Autorzy:
Kurnik, K.
Skorupa-Klaput, M.
Tyburski, J.
Powiązania:
https://bibliotekanauki.pl/articles/80526.pdf
Data publikacji:
2013
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
conference
ascorbate peroxidase
sugar-beet
Beta vulgaris
Escherichia coli
expression
peroxidase activity
protein extract
BL21 cell
Źródło:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2013, 94, 3
0860-7796
Pojawia się w:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Bundle sheath-specific expression of the ascorbate peroxidase 2 affects photosynthesis in whole plant
Autorzy:
Gorecka, M.
Alvarez Fernandez, R.
Bialasek, M.
Mulleineaux, P.
Karpinski, S.
Powiązania:
https://bibliotekanauki.pl/articles/951327.pdf
Data publikacji:
2013
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
conference
sheath-specific expression
ascorbate peroxidase
hydrogen peroxide
photosynthesis
cytosolic peroxidase
fluorescence
electron transport chain
gene encoding
Źródło:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2013, 94, 2
0860-7796
Pojawia się w:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Deuterium isotope effects in oxidation of dopamine and norepinephrine catalyzed by horseradish peroxidase
Autorzy:
Byszewska, W.
Pająk, M.
Kańska, M.
Powiązania:
https://bibliotekanauki.pl/articles/146852.pdf
Data publikacji:
2013
Wydawca:
Instytut Chemii i Techniki Jądrowej
Tematy:
deuterium
dopamine (DA)
norepinephrine
isotope effects
oxidation
peroxidase
Opis:
Deuterium kinetic isotope effects (KIE’s) in the reaction of oxidation of dopamine (DA) catalyzed by the enzyme horseradish peroxidase (HRP, EC 1.11.1.7) was determined using a non-competitive spectrophotometric method. For kinetic studies, the deuterium ring labelled isotopomer [2’,5’,6’-2H3]-dopamine was synthesized using acid catalyzed isotopic exchange between authentic dopamine and heavy water. Deuterium solvent isotope effects (SIE’s) for dopamine and norepinephrine were determined separately for the enzymatic oxidation carried out in the presence or absence of hydrogen peroxide. Some mechanistic details of enzymatic oxidation of dopamine and norepinephrine to corresponding catecholchromes catalyzed by HRP were discussed.
Źródło:
Nukleonika; 2013, 58, 4; 475-479
0029-5922
1508-5791
Pojawia się w:
Nukleonika
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Effect of temperature on guaiacol peroxidase of Pyrus communis
Autorzy:
Saeidian, S.
Ghasemifar, E.
Powiązania:
https://bibliotekanauki.pl/articles/10793.pdf
Data publikacji:
2013
Wydawca:
Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
Tematy:
temperature effect
guaiacol
Peroxidase
Pyrus communis
enzyme
plant growth
plant development
cell wall
Opis:
Peroxidase (EC 1.11.1.7; donor: hydrogen-peroxide oxidoreductase, POD) is one of the key enzymes controlling plant growth, differentiation and development. The enzyme participates in construction, rigidification and eventual lignification of cell walls, biosynthesis of ethylene from 1-aminocyclopropane-1-carboxylic acid and H2O2, regulation of auxin level through auxin catabolism, protection of tissue from damage and infection by pathogenic microorganisms, the oxidation of indoleacetic acid. For peroxidase activity in wild pears extract one pH optimum was observed at 6.5 that probably belong to atleast one isoenzyme. Activity of peroxidase in presence of guaiacol and H2O2 was optimum after incubation at 40 °C. Maximum activity of peroxidase is 300 % .Activity increased to 240 %, 300 %, 70 % and 10 % after 60 minute incubation at 30, 40, 45 and 60 °C for peroxidase. Incubation at high temperature (70 °C) was accompanied with decrease of activity to 10 % peroxidase activity.
Źródło:
International Letters of Natural Sciences; 2013, 05
2300-9675
Pojawia się w:
International Letters of Natural Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Cadmium-induced changes in antioxidant enzymes in suspension culture of soybean cells.
Autorzy:
Sobkowiak, Robert
Rymer, Katarzyna
Rucińska, Renata
Deckert, Joanna
Powiązania:
https://bibliotekanauki.pl/articles/1043349.pdf
Data publikacji:
2004
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
ascorbate peroxidase
superoxide dismutase
peroxidase
soybean cell suspension culture
catalase
cadmium
Opis:
Cadmium (Cd), similarly to other heavy metals, inhibits plant growth. We have recently showed that Cd2+ either stimulates (1-4 μM) or inhibits (ł 6 μM) growth of soybean (Glycine max L.) cells in suspension culture (Sobkowiak & Deckert, 2003, Plant Physiol Biochem. 41: 767-72). Here, soybean cell suspension cultures were treated with various concentrations of Cd2+ (1-10 μM) and the following enzymes were analyzed by native electrophoresis: superoxide dismutase (SOD), catalase (CAT), peroxidase (POX) and ascorbate peroxidase (APOX). We found a significant correlation between the cadmium-induced changes of soybean cell culture growth and the isoenzyme pattern of the antioxidant enzymes. The results suggest that inhibition of growth and modification of antioxidant defense reactions appear in soybean cells when Cd2+ concentration in culture medium increases only slightly, from 4 to 6 μM.
Źródło:
Acta Biochimica Polonica; 2004, 51, 1; 219-222
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Effect of nanosilver on physiological performance of pelargonium plants exposed to dark storage
Autorzy:
Hatami, M.
Ghorbanpour, M.
Powiązania:
https://bibliotekanauki.pl/articles/1826.pdf
Data publikacji:
2013
Wydawca:
Instytut Ogrodnictwa
Tematy:
Pelargonium
Pelargonium x hortorum
nanosilver
leaf senescence
storage
antioxidative enzyme
lipid peroxidation
ascorbate peroxidase
guaiacol peroxidase
Źródło:
Journal of Horticultural Research; 2013, 21, 1
2300-5009
Pojawia się w:
Journal of Horticultural Research
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Blood leukocytes and eosinophil peroxidase are not related to faecal egg counts in Polish Wrzosowka hoggets naturally infected with gastrointestinal nematodes
Autorzy:
Moskwa, B.
Doligalska, M.
Cabaj, W.
Powiązania:
https://bibliotekanauki.pl/articles/839118.pdf
Data publikacji:
1998
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
parasite
gastrointestinal nematode
blood
nematode
leucocyte
eosinophil peroxidase
Źródło:
Annals of Parasitology; 1998, 44, 3
0043-5163
Pojawia się w:
Annals of Parasitology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Effect of copper(II) the activity of glutathione peroxidase in patients with head and neck cancer
Autorzy:
Malinowska, Katarzyna
Morawiec-Sztandera, Alina
Majsterek, Ireneusz
Kaczmarczyk, Dariusz
Powiązania:
https://bibliotekanauki.pl/articles/1398625.pdf
Data publikacji:
2016
Wydawca:
Index Copernicus International
Tematy:
pyrazole complex
glutathione peroxidase
head and neck cancer
Opis:
Introduction: Head and neck squamous cell carcinoma (HNSCC) accounts for about 6% of all malignant cancers. In the epidemiology of oral cavity neoplasm, important risk factors include: tobacco smoking, alcohol abuse, bad oral hygiene, papilloma virus infection, riboflavin and iron deficiency. Objective: The objective of the investigation was a synthesis of Cu(II) complex and the evaluation of antioxidative enzymatic barrier in red blood cells of patients with head and neck tumor as well as in the control group. Materials and methods: For the investigation conduction, a consent of Bioethics Committee number RNN/142/09/KB was obtained. Blood for the examination was obtained from the patients of the Dapartment of Head and Neck Neoplasms Surgery Medical University of Łódź. The experiment was conducted on the group of 40 patients with HNSCC and 40 healthy people, using spectrophotometric method, glutathione peroxidase was marked. Results: The investigation was conducted on the hemolysate obtained from the patients that were divided into two groups – a study group (1 and 2), which consisted of patients diagnosed with head and neck cancer and a control group (1 and 2) – healthy people. A significant statistical result for GPX occurred in control-1 and study-1 group with complex compound Cu(II) (p<0,001). Conclusions: Presented research prove, that complex compound Cis-dichlorobis(N1-hydroxymethyl-3methylpyrazole-κN2)copper (II) has an impact on the activity of the antioxidative GPX enzyme.
Źródło:
Polish Journal of Otolaryngology; 2016, 70, 6; 20-25
0030-6657
2300-8423
Pojawia się w:
Polish Journal of Otolaryngology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Grain discoloration in different genotypes of durum wheat (Triticum durum L.) in Argentina: associated mycobiota and peroxidase activity
Autorzy:
Cipollone, M.J.
Moya, P.
Martinez, I.
Saparrat, M.
Sisterna, M.
Powiązania:
https://bibliotekanauki.pl/articles/2084746.pdf
Data publikacji:
2020
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
durum wheat
grain discoloration
mycobiota
peroxidase activity
Opis:
Wheat grain discoloration, a worldwide disease that lowers grain quality and decreases grain yield, does not have a single etiology. It has been proposed that it is a consequence of an abiotic mechanism, a response to environmental conditions or enzymatic activity. It has also been suggest that it is a biotic mechanism, a fungal infection principally by Alternaria spp. and Bipolaris sorokiniana. The present work was carried out to analyze the possible etiology of this disease in nine durum wheat genotypes from two localities of southern Buenos Aires province (Argentina) on two sowing dates. Incidence (percentage of grain discoloration) was recorded and mycobiota associated with this pathology was registered following ISTA rules. Peroxidase activity in an extract obtained from grains belonging to genotypes of the locality that showed the highest incidence was measured. The incidence among genotypes, localities and sowing dates varied, although the genotypes with the higher and lower values of incidence were the same for all the variables tested. The fungus Alternaria spp. was isolated the most frequently followed by Fusarium spp., while Bipolaris sorokiniana was found the least frequently. Peroxidase activity showed that all the treatments had similar levels of enzymatic activity, but there was no clear differentiation between controls either between genotypes with the lowest or the highest incidence values. This suggests that peroxidase activity did not have a clear relationship with grain discoloration. In this research, it is presumed that fungal infection is the main cause of this disease.
Źródło:
Journal of Plant Protection Research; 2020, 60, 1; 14-20
1427-4345
Pojawia się w:
Journal of Plant Protection Research
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Red blood cell and plasma glutathione peroxidase activities and selenium concentration in patients with chronic kidney disease: A review
Autorzy:
Zachara, Bronisław
Gromadzińska, Jolanta
Wąsowicz, Wojciech
Zbróg, Zbigniew
Powiązania:
https://bibliotekanauki.pl/articles/1041155.pdf
Data publikacji:
2006
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
hemodialysis
selenium
kidney transplantation
glutathione peroxidase
chronic kidney disease
antioxidants
plasma
Opis:
The metabolism of oxygen in aerobic organisms leads to generation of reactive oxygen species (ROS). These entities are able to oxidize almost all classes of macromolecules, including proteins, lipids and nucleic acids. The physiological level of ROS is usually regulated by antioxidant defense mechanisms. There are at least three groups of antioxidant enzymes: superoxide dismutases, catalases and glutathione peroxidases (GSH-Pxs) which neutralize ROS. The trace elements (copper, zinc and selenium) bound to the active sites of the above listed enzymes play an important role in the antioxidant defense system. In mammals, a major function of selenium (Se) and Se-dependent GSH-Pxs is to protect cells from oxidative stress. Selenium concentrations and GSH-Px activities are altered in blood components of chronic kidney disease (CKD) patients. The Se level is frequently lower than in healthy subjects and the concentration very often decreases gradually with advancing stage of the disease. Studies on red cell GSH-Px activity in CKD patients reported its values significantly lower, significantly higher and lower or higher, but not significantly as compared with healthy subjects. On the other hand, all authors who studied plasma GSH-Px activity have shown significantly lower values than in healthy subjects. The degree of the reduction decreases gradually with the progression of the disease. High inverse correlations were seen between plasma GSH-Px activity and creatinine level. A gradual decrease in plasma GSH-Px activity in CKD patients is due to the fact that this enzyme is synthesized predominantly in the kidney and thus the impairment of this organ is the cause of the enzyme's lower activity. Se supplementation to CKD patients has a slightly positive effect in the incipient stage of the disease, but usually no effect was observed in end-stage CKD. Presently, kidney transplantation is the only treatment that may restore plasma Se level and GSH-Px activity in patients suffering from end-stage CKD. A few studies have shown that in kidney recipients, plasma Se concentration and GSH-Px activity are restored to normal values within a period of 2 weeks to 3 months following surgery and thus it can be acknowledged that Se supplementation to those patients has a positive effect on plasma GSH-Px activity.
Źródło:
Acta Biochimica Polonica; 2006, 53, 4; 663-677
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Differential response of antioxidant enzymes to cadmium stress in tolerant and sensitive cell line of cucumber (Cucumis sativus L.)
Autorzy:
Gzyl, Jarosław
Rymer, Katarzyna
Gwóźdź, Edward
Powiązania:
https://bibliotekanauki.pl/articles/1040497.pdf
Data publikacji:
2009
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
cell suspension
cadmium
tolerance
superoxide dismutase
catalase
ascorbate peroxidase
guaiacol peroxidase
Opis:
Previously, a stable cell suspension of cucumber tolerant to 100 µM CdCl2 was obtained (Gzyl & Gwóźdź, 2005, Plant Cell Tissue Organ Cult 80: 59-67). In this study, the relationship between the activity of antioxidant enzymes and cadmium tolerance of cucumber cells was analyzed. A cadmium-sensitive and the cadmium-tolerant cell lines were exposed to 100 µM and 200 µM CdCl2 and the activities of superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APOX) and guaiacol peroxidase (POX) were determined. In the sensitive cell line, a decrease of total activity of SOD and POX was observed, whereas the activity of CAT and APOX significantly increased in metal-supplemented medium. By contrast, in the tolerant cells, the total activity of antioxidant enzymes decreased (SOD, CAT) or was maintained at approximately the same level (APOX, POX). Moreover, a different pattern of isoenzyme activity was observed in the tolerant and sensitive cells. These results suggest that an enhanced activity of antioxidant enzymes is not directly involved in the increased tolerance to cadmium of the selected cucumber cell line.
Źródło:
Acta Biochimica Polonica; 2009, 56, 4; 723-727
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Porównanie oddziaływania selenu na aktywność peroksydazową gleby skażonej olejem napędowym lub przepracowanym olejem silnikowym
Comparison of selenium effect on peroxidase activities in soil contaminated with diesel oil and spent engine oil
Autorzy:
Strek, M.
Telesinski, A.
Powiązania:
https://bibliotekanauki.pl/articles/36028.pdf
Data publikacji:
2016
Wydawca:
Polska Akademia Nauk. Instytut Agrofizyki PAN
Tematy:
gleby
selen
zanieczyszczenia gleb
olej napedowy
olej silnikowy
olej silnikowy przepracowany
peroksydaza
aktywnosc peroksydazowa
soil
selenium
soil contaminant
diesel fuel
engine oil
peroxidase
peroxidase activity
Opis:
W pracy przedstawiono wyniki oddziaływania oleju napędowego, przepracowanego oleju silnikowego oraz selenu (IV i VI), na aktywność peroksydazową gleby. Doświadczenie przeprowadzono w warunkach laboratoryjnych na piasku gliniastym o zawartości węgla organicznego 8,7 g·kgˉ¹. Do próbek gleby wprowadzono w różnych kombinacjach kwas selenowy (IV) lub kwas selenowy (VI) (ilość dodanego Se wynosiła 0,05 mmol·kgˉ¹) oraz przepracowany olej silnikowy lub olej napędowy w ilościach 2, 10 i 50 g·kgˉ¹. Wszystkie próbki doprowadzono do 60% maksymalnej pojemności wodnej i przechowywano w szklanych pojemnikach typu twist, w stałej temperaturze 20°C. Aktywność peroksydaz oznaczono spektrofotometrycznie w 1., 7., 14., 28., 56. i 112. dniu doświadczenia. Skażenie gleby olejem napędowym oraz przepracowanym olejem silnikowym spowodowało stymulację aktywności peroksydaz. Wprowadzenie selenu do gleby nieskażonej substancjami ropopochodnymi wywołało zmiany aktywności peroksydaz. Jedynie dodatek selenu VI do gleby zawierającej olej napędowy w dawce 2 g·kgˉ¹ spowodował istotny wzrost średniej aktywności peroksydaz glebowych.
This paper describes the impact of diesel oil, spent engine oil and selenium (IV and VI) on peroxidase activity in soil. Experiment was carried out in laboratory conditions on loamy sand with organic carbon content of 8.7 g·kgˉ¹. Different combinations of selenic (IV) acid or selenic (VI) acid (the Se amount was 0.05 mmol·kgˉ¹) and diesel oil or spent engine oil at dosage of 2, 10 i 50 g·kgˉ¹ were added to soil samples. All samples were adjusted to 60% of the maximum water holding capacity and stored in glass twist containers at a temperature of 20°C. Activity of peroxidases was determined spectrophotometrically on days 1, 7, 14, 28, 56 and 112. Soil contamination with diesel oil and spent engine oil increased the activity of peroxidases. Application of selenium to soil uncontaminated with petroleum hydrocarbons caused significant changes of peroxidase activities in the initial period of experiment. Only in soil containing diesel oil at the dosage of 2 g·kgˉ¹ significant increase in activity of peroxidase was observed after treatment with selenium VI.
Źródło:
Acta Agrophysica; 2016, 23, 3
1234-4125
Pojawia się w:
Acta Agrophysica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Właściwości fizykochemiczne produktów enzymatycznego utleniania skrobi
Physicochemical properties of products of enzymatic oxidation of starch
Autorzy:
Roczkowska, M.
Siemion, P.
Kaczmarek, A.
Powiązania:
https://bibliotekanauki.pl/articles/410267.pdf
Data publikacji:
2013
Wydawca:
Uniwersytet Humanistyczno-Przyrodniczy im. Jana Długosza w Częstochowie. Wydawnictwo Uczelniane
Tematy:
skrobia
skrobia utleniona
utlenianie enzymatyczne
peroksydaza ligninowa
LiP
peroksydaza manganozależna
MnP
starch
oxidized starch
enzymatic oxidation
lignin peroxidase
manganese peroxidase
Opis:
Reakcje enzymatyczne coraz częściej uzupełniają i zastępują wszelkie reakcje chemiczne. Są wysoce selektywne i precyzyjne, gdyż enzymy działają na konkretne grupy czy wiązania chemiczne. Przykładem enzymów wykorzystywanych w procesach utleniania są lakazy oraz peroksydazy – ligninowa (LiP) i manganozależna (MnP). Niniejsza praca została poświęcona badaniom właściwości fizykochemicznych skrobi utlenionych enzymatycznie przy użyciu peroksydazy ligninowej (LiP) oraz manganozależnej (MnP). Badaniom zostały poddane utlenione skrobie kukurydziane oraz ziemniaczane. Badania wykazały, że we wszystkich analizowanych próbkach doszło do utlenienia enzymatycznego. Produkty modyfikacji odznaczały się odmiennymi właściwościami fizykochemicznymi w stosunku do próbek natywnych skrobi tego samego gatunku. Zmianie uległa zdolność pochłaniania wody, rozpuszczalność w wodzie i trwałość w wysokich temperaturach.
The enzymatic reactions increasingly replace and complement any chemical reactions. They are highly selective and precise, because the enzymes act on specific groups or chemical bonds. The examples of enzymes applied in the oxidation processes are laccases, lignin (LiP) and manganese (MnP) peroxidases. This work has been devoted to the studies on physicochemical properties of the enzymatically oxidized starches using lignin (LiP) and manganese (MnP) peroxidases. The studies were performed on oxidized corn and potato starches. The studies have shown that the enzymatic oxidation had occurred in all of the analyzed samples. The products of modification showed different physicochemical properties in comparison to native starch samples of the same species. The water absorption capacity, water solubility and stability at high temperatures have been altered.
Źródło:
Chemistry, Environment, Biotechnology; 2013, 16; 85-96
2083-7097
Pojawia się w:
Chemistry, Environment, Biotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Peroxidase activity and soluble carbohydrates content in pea roots in response to soil contamination with oxytetracycline
Autorzy:
Dobiesz, M.
Ziolkowska, A.
Piotrowicz-Cieslak, A.I.
Powiązania:
https://bibliotekanauki.pl/articles/80780.pdf
Data publikacji:
2013
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
conference
peroxidase activity
carbohydrate content
pea
soil contamination
oxytetracycline
Streptomyces rimosus
bacteriostatic activity
antioxidative enzyme
Źródło:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2013, 94, 3
0860-7796
Pojawia się w:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Can changes in starch content and peroxidase activity be used as rooting phase markers for rhododendron leaf bud cuttings?
Autorzy:
Megre, D.
Dokane, K.
Kondratovics, U.
Powiązania:
https://bibliotekanauki.pl/articles/19397.pdf
Data publikacji:
2011
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
content change
starch content
peroxidase activity
rooting phase marker
rhododendron
leaf bud cutting
propagation
rooting
Opis:
We examined whether peroxidase activity in cutting bases and leaves and starch content in cutting bases can be used as rooting phase markers in the elepidote rhododendron cv. 'Babites Baltais' (Rhododendron L.). Changes in peroxidase activity in cutting leaves and bases, as well as starch content in cutting bases, were determined in relation to anatomical stages of rhizogenesis in leaf bud cuttings treated with 1% indole-3-butyric acid (IBA+) or without IBA (IBA–). The pattern of change of peroxidase activity was similar in cutting bases and leaves of IBAleaf bud cuttings. Three phases of adventitious root formation were identified: induction, initiation and expression. During the induction phase peroxidase activity decreased, but no anatomical changes were observed in the cuttings. Peroxidase activity increased in the initiation phase when adventitious root initials were formed. Peroxidase activity decreased during the expression phase when adventitious root primordia developed. The starch content of IBA– leaf bud cuttings decreased during the first few days and then gradually rose to maximum, followed by a sharp reduction and another increase at the end of the experiment. The changes of starch content did not coincide with rooting phases as peroxidase activity did, and cannot be used as a rooting phase marker in rhododendrons. Adventitious root formation did not occur in IBA+ leaf bud cuttings, so distinct rooting phases could not be observed. There was a significant correlation between peroxidase activity in cutting bases and leaves of IBA- leaf bud cuttings. Peroxidase activity in leaves of rhododendron leaf bud cuttings are potentially useful as a marker for rooting phases, but that requires further anatomical and physiological study of rooting in leaf bud cuttings.
Źródło:
Acta Biologica Cracoviensia. Series Botanica; 2011, 53, 1
0001-5296
Pojawia się w:
Acta Biologica Cracoviensia. Series Botanica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The effect of methylprednisolone on the dynamics of oxygen uptake and peroxidase and superoxide dismutase activities in skeletal muscles of mice infected with Trichinella spiralis larvae
Autorzy:
Wandurska-Nowak, E.
Boczon, K.
Derda, M.
Powiązania:
https://bibliotekanauki.pl/articles/838559.pdf
Data publikacji:
1998
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
parasite
superoxide dismutase
mouse
mice
larva
peroxidase
Trichinella spiralis
skeletal muscle
Źródło:
Annals of Parasitology; 1998, 44, 3
0043-5163
Pojawia się w:
Annals of Parasitology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Superoxide dismutase and glutathione peroxidase activity in pregnancy complicated by diabetes
Autorzy:
Wdowiak, A.
Brzozowski, I.
Bojar, I.
Powiązania:
https://bibliotekanauki.pl/articles/50976.pdf
Data publikacji:
2015
Wydawca:
Instytut Medycyny Wsi
Źródło:
Annals of Agricultural and Environmental Medicine; 2015, 22, 2
1232-1966
Pojawia się w:
Annals of Agricultural and Environmental Medicine
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Selenium supplementation to chronic kidney disease patients on hemodialysis does not induce the synthesis of plasma glutathione peroxidase
Autorzy:
Zachara, Bronislaw
Gromadzinska, Jolanta
Zbrog, Zbigniew
Swiech, Rafal
Wasowicz, Wojciech
Twardowska, Ewa
Jablonska, Ewa
Sobala, Wojciech
Powiązania:
https://bibliotekanauki.pl/articles/1040656.pdf
Data publikacji:
2009
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
hemodialysis
selenium supplementation
glutathione peroxidase
chronic kidney disease
plasma
Opis:
Background: Numerous authors have shown that selenium (Se) concentration and glutathione peroxidase (GSH-Px) activity in plasma of chronic kidney disease (CKD) patients are lower than in healthy subjects, but there are only few publications on the level of GSH-Px protein in those patients and no reports on the effect of Se supplementation to HD patients on the level of this enzyme. Subjects and Methods: Se concentration and GSH-Px protein level in plasma were measured in a group of 30 CKD patients on hemodialysis (HD) supplemented with 200 µg Se/day for 3 months, and 28 patients on HD administered with placebo. Se concentration was measured by graphite furnace atomic absorption spectrometry and plasma GSH-Px protein level by the sandwich ELISA method using polyclonal antibody specific for human plasma GSH-Px. Results: Se concentration in patients on placebo did not change throughout the 3-month study period, but increased significantly in Se supplemented group. Se supplementation to CKD patients on HD had no effect on the level of GSH-Px protein. Conclusions: The lack of GSH-Px protein in CKD patients on HD is not linked to Se deficiency since the level of this element increased after Se supplementation while enzyme protein level did not change. The damaged kidney of HD patients is unable to synthesize GSH-Px, even after induction with selenium.
Źródło:
Acta Biochimica Polonica; 2009, 56, 1; 183-187
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Ligninolityczne właściwości izolatów Heterobasidion parviporum w hodowli na drewnie świerka
Ligninolytic activity of Heterobasidion parviporum isolates in cultivation on Norway spruce wood
Autorzy:
Żółciak, A.
Bohacz, J.
Powiązania:
https://bibliotekanauki.pl/articles/989467.pdf
Data publikacji:
2016
Wydawca:
Polskie Towarzystwo Leśne
Tematy:
lesnictwo
fitopatologia lesna
grzyby
Heterobasidion parviporum
izolaty grzybowe
aktywnosc lignolityczna
enzymy lignolityczne
lakaza
peroksydaza manganozalezna
peroksydaza ligninowa
peroksydaza "versatile"
rozklad drewna
drewno bielu
drewno twardzieli
drewno swierkowe
biodegradacja
laccase
manganese peroxidase
lignin peroxidase
versatile peroxidase
hydroxy−
methoxyphenols
sapwood
heartwood
norway spruce wood
Opis:
The aim of this preliminary study was to assess the activity of laccase, diverse peroxidases as well as the level of micromolecular compounds in Heterobasidion parviporum isolates grown on pieces of Norway spruce wood (sapwood and heartwood) during 50 days of incubation under the laboratory conditions. H. parviporum isolates secreted extracellular enzymes: laccase, manganese peroxidase (MnP), lignin peroxidase (LiP) and versatile peroxidase (VP). Hydroxy− and methoxyphenols were also released during this process. The above−mentioned enzymes showed low activity in mycelium grown on both sapwood and heartwood. The activity of laccase ranged from 0 to 0.513 mU/mg protein on sapwood, and from 0 to 0.106 mU/mg protein – on heartwood. MnP activity of H. parviporum isolates ranged from 0.024 to 0.667 mU/mg protein on sapwood, and from 0.038 to 1.585 mU/mg protein – on heartwood. LiP activity was small and ranged from 0 to 1.281 mU/mg protein on sapwood, and from 0.013 to 0.166 mU/mg protein – on heartwood. Activity of VP oxidizing manganese ions was low. It ranged from 0 to 3.063 mU/mg protein on sapwood, and from 0.059 to 3.054 mU/mg protein – on heartwood. The activity of VP oxidizing guaiacol ranged from 0.006 to 1.490 mU/mg protein on sapwood and from 0.038 to 1.147 mU/mg protein – on heartwood. The hydroxyphenols produced by H. parviporum isolates ranged from 15.037 to 110.149 mg of protocatechuic acid/ml on sapwood, and from 11.236 to 27.220 mg of protocatechuic acid/ml – on heartwood. Methoxyphenols produced by H. parviporum isolates ranged from 3.393 to 24.253 mg of vanillic acid/ml on sapwood, and from 4.955 to 12.005 mg of vanillic acid/ml – on heartwood.
Źródło:
Sylwan; 2016, 160, 12; 1027-1035
0039-7660
Pojawia się w:
Sylwan
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Identification of Heterobasidion spp.in Poland by RFLP analysis of laccase and manganese dependent peroxidase
Autorzy:
Kraj, W.
Kowalski, T.
Powiązania:
https://bibliotekanauki.pl/articles/41587.pdf
Data publikacji:
2010
Wydawca:
Polska Akademia Nauk. Instytut Dendrologii PAN
Opis:
Laccase and manganese dependent peroxidase (MnP) genes in H. annosum s.l. were studied by PCR-RFLP. The peroxidase genes MNP1a and MNP2 showed Heterobasidion species specific length differences. Among eighteen monomorphic markers which were foundfor investigatedgenes, three were characteristic for H. abietinum andfive for H. annosum s.s. The remaining specific markers were characteristic for H. parviporum and H. abietinum (nine markers) or for H. parviporum and H. annosum s.s. (one marker). No specific marker for H. parviporum was detected. On the basis of monomorphic markers, intersterility groups of eighty one strains isolatedfrom eleven forest tree or bush species in south Polandwere identified. Fifty-three belongedto H. annosum s.s., twenty-five to H. parviporum, andthree to H. abietinum. Strains belonging to H. annosum s.s. were isolatedfrom Pinus nigra, P. strobus, P. sylvestris, Larix decidua, Picea abies, as well as from Alnus incana, Betula pendula, Padus avium, Quercus robur and Q. rubra. H. parviporum was isolatedfrom Abies alba, Picea abies, Pinus nigra, P. sylvestris, Larix decidua andfrom Alnus incana. This is the first report of H. parviporum occurrence on Alnus incana in Poland. The ascertained H. abietinum strains derived from Abies alba. Our results demonstrate a new methodof Heterobasidion species identification based on the different length of MNP1a and MNP2 peroxidase genes and RFLP markers obtained for laccase and Mn2+ dependent peroxidase genes.
Źródło:
Dendrobiology; 2010, 63
1641-1307
Pojawia się w:
Dendrobiology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Betacyanin accumulation and guaiacol peroxidase activity in Beta vulgaris L. leaves following copper stress
Autorzy:
Morales, J.M.L.
Rodriguez-Monroy, M.
Sepulveda-Jimenez, G.
Powiązania:
https://bibliotekanauki.pl/articles/57381.pdf
Data publikacji:
2012
Wydawca:
Polskie Towarzystwo Botaniczne
Tematy:
betacyanin accumulation
guaiacol peroxidase activity
Beta vulgaris
red beet
leaf
copper stress
oxidative stress
betacyanin
abiotic stress
antioxidant compound
lipid peroxidation
copper
Opis:
The effect of copper stress on betacyanin accumulation and guaiacol peroxidase (GPOD) activity in leaves of different age was evaluated in red beet (Beta vulgaris L. var. Crosby Egyptian) plants. In hydroponic culture, plants were treated with 0.3 μM (control), 50 μM, 100 μM, and 250 μM of CuSO4 for 6 days. Copper was taken up and accumulated in old roots but was not translocated to leaves. However in young leaves, the increase of lipid peroxidation and reduction of growth were evident from day 3 of copper exposure; whereas in old leaves, the lipid peroxidation and growth were the same from either copper-treated or control plants. In response to copper exposure, the betacyanin accumulation was evident in young leaves by day 3, and continued to increase until day 6. Betacyanin only were accumulated in old leaves until day 6, but the contents were from 4 to 5 times lower than those observed in young leaves at the same copper concentrations. GPOD activity increased 3.3- and 1.4-fold in young and old leaves from day 3 of copper treatment respectively, but only in the young leaves was sustained at the same level until day 6. Old roots shown betacyanin in the control plants, but the betacyanin level and growth were reduced with the copper exposure. In contrast, young roots emerged by copper effect also accumulated copper and showed the highest betacyanin content of all plant parts assayed. These results indicate that betacyanin accumulation and GPOD activity are defense responses to copper stress in actively growing organs.
Źródło:
Acta Societatis Botanicorum Poloniae; 2012, 81, 3
0001-6977
2083-9480
Pojawia się w:
Acta Societatis Botanicorum Poloniae
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Changes of antioxidant enzymes of mung bean [Vigna radiata (L.) R. Wilczek] in response to host and non-host bacterial pathogens
Autorzy:
Farahani, A.S.
Taghavi, M.
Powiązania:
https://bibliotekanauki.pl/articles/65764.pdf
Data publikacji:
2016
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
antioxidative enzyme
enzyme
mung bean
Vigna radiata
plant response
host pathogen
non-host pathogen
resistance
bacterial pathogen
ascorbate peroxidase
catalase
peroxidase
superoxide dismutase
Opis:
The natural resistance against the majority of potential pathogens that exist in most plant species is known as non-host resistance. Several reports suggest the role of antioxidant enzymes in non-host resistance. We assayed the expression or activity of four scavenging enzymes during non-host pathogen-plant interaction (Xanthomonas hortorum pv. pelargonii/mung bean) and host pathogen-plant interaction (Xanthomonas axonopodis pv. phaseoli/mung bean). The expression of superoxide dismutase (SOD) and ascorbate peroxidase (APX) and the enzyme activity of catalase (CAT) and peroxidase (POX) were investigated. The activities of CAT and POX were higher during non-host pathogen invasion vs. host pathogen attack. The expression of SOD and APX were also different between compatible and incompatible interactions. The expression of SOD and APX were higher in the incompatible compared to the compatible interaction. Additionally, induction of the antioxidant enzymes in response to non-host pathogen was earlier than induction in response to host pathogen. Such information is important for plant breeders, and useful when looking for alternative control strategies as well.
Źródło:
Journal of Plant Protection Research; 2016, 56, 1
1427-4345
Pojawia się w:
Journal of Plant Protection Research
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Effect of priming of seeds of Medicago sativa "Bami" with gibberellic acid on germination, seedlings growth and antioxidant enzymes activity under salinity stress
Autorzy:
Younesi, O.
Moradi, A.
Powiązania:
https://bibliotekanauki.pl/articles/1828.pdf
Data publikacji:
2014
Wydawca:
Instytut Ogrodnictwa
Tematy:
salinity
seed priming
Medicago sativa
gibberellic acid
germination
seedling growth
growth
antioxidative enzyme
alfalfa
guaiacol peroxidase
catalase
ascorbate peroxidase
glutathione reductase
Źródło:
Journal of Horticultural Research; 2014, 22, 2
2300-5009
Pojawia się w:
Journal of Horticultural Research
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Characterization and analyses of hydrophobic clusters, acetylation and myristoylation sites in plant glutathione peroxidase sequences
Autorzy:
Ganguli, S.
Polley, A.
Datta, A.
Powiązania:
https://bibliotekanauki.pl/articles/10847.pdf
Data publikacji:
2015
Wydawca:
Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
Opis:
Incomplete reduction of oxygen molecules is the primary source for the formation of reactive oxygen species (ROS) during cytosolic metabolism or mitochondrial respiration. These phenomenons may be as a result of biotic or abiotic stress. Exposure to exogenous stimuli such as radiation might be an alternative pathway of ROS production. Thus plants require counter defense strategies to combat the increase of this toxic molecular build up in its cell cytoplasm. As a result they have devised an army of free radical scavenging enzymes which enable them to dissipate the oxidative stress imposed by the accumulation of these toxic moieties. Glutathione Peroxidase forms an important part of this arms race along with several catalases and organelle specific enzymes such as superoxide dismutase. Plant glutathione peroxidases(GPXs) have been studied exclusively for their evolutionary lineages since they represent a hybrid class of molecules in context of the presence and absence of selenocysteine at their catalytic centres, the former situation predominant in non vascular plant groups while the later a predominant feature of vascular plants. This analysis focuses on three important aspects of protein structure analyses – hydrophobic cluster analyses for identification of homologues, and acetylation and myristoylation sites which provide us with information regarding the post translational modifications of a particular protein group. Specific patterns of clusters along with acetylation and myristoylation site frequencies were obtained which indicate that GPXs of non vascular plant members possess less chances of getting myristoylated while acetylation was predominant in most land plant lineages but absent in aquatic members.
Źródło:
International Letters of Natural Sciences; 2015, 01
2300-9675
Pojawia się w:
International Letters of Natural Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The effect of seedling chilling on glutathione content, catalase and peroxidase activity in Brassica oleracea L. var. italica
Autorzy:
Wojciechowska, R.
Hanus-Fajerska, E.J.
Kolton, A.
Kaminska, I.
Grabowska, A.
Kunicki, E.
Powiązania:
https://bibliotekanauki.pl/articles/57934.pdf
Data publikacji:
2013
Wydawca:
Polskie Towarzystwo Botaniczne
Tematy:
seedling
chilling stress
glutathione content
catalase activity
peroxidase activity
Brassica oleracea var.italica
broccoli
development stage
Opis:
The study was designed to determine the possible relationship between Brassica oleracea var. italica seedlings stored at 2°C in the dark for seven and fourteen days, respectively, and the level of certain antioxidant parameters in particular organs. A parallel objective of the experiment was to determine if the reaction of seedlings to low temperature might be persistent in fully developed plants until harvest time. After 14 days of chilling a significant increase in the glutathione content was observed in the seedling leaves in comparison to the non-chilled plants. During vegetation in field conditions this effect was maintained in leaves up to the stage of formation of flower buds. At harvest the highest content of glutathione was demonstrated in broccoli heads, obtained from plants, which were previously chilled in the seedling phase for two weeks. Peroxidase activity in broccoli seedlings increased each year of the three-year study due to the duration of the cooling time, whereas in the case of catalase the changes were not so distinct. At harvest time the activity of both enzymes in the leaves and flower buds fluctuated according to the particular year of study.
Źródło:
Acta Societatis Botanicorum Poloniae; 2013, 82, 3
0001-6977
2083-9480
Pojawia się w:
Acta Societatis Botanicorum Poloniae
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Activity of superoxide dismutase, catalase and glutathione peroxidase in rats exposed to chlorpyrifos and erofloxacin
Autorzy:
Barski, D.
Spodniewska, A.
Zasadowski, A.
Powiązania:
https://bibliotekanauki.pl/articles/32363.pdf
Data publikacji:
2011
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
The aim of the study was to investigate the influence of administration of chlorpyrifos and/or enrofloxacin on the activity of chosen antioxidative enzymes i.e.: superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx) in erythrocytes of rats. Chlorpyrifos was administered by stomach tube during 28 days at a dose of 3 mg/kg bw (0.02 LD50), and enrofloxacin was administered by stomach tube at a dose of 5 mg/kg bw during 3 subsequent days. It was stated that administration of enrofloxacin at applied dose did not cause any major changes in the activity of investigated antioxidative enzymes. The four-week exposure of rats to chlorpyrifos caused noticeable decrease in SOD and CAT activity in erythrocytes of rats at the beginning of the experiment (up to 24th hour) in comparison with the control group. The activity of GPx during all periods of the experiment was increased. In the group of animals in which both chlorpyrifos and enrofloxacin were applied, the profile of changes in activity of examined enzymes was similar to that one, which was observed after administration of chlorpyrifos exclusively, what may indicate lack of co-action between compounds used in the experiment.
Źródło:
Polish Journal of Veterinary Sciences; 2011, 14, 4
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Effect of 8-hydroxyquinoline citrate, sucrose and peroxidase inhibitors on vase life of Lisianthus (Eustoma grandiflorum L.) cut flowers
Autorzy:
Sharifzadeh, K.
Asil, M.H.
Roein, Z.
Sharifzadeh, M.
Powiązania:
https://bibliotekanauki.pl/articles/2024.pdf
Data publikacji:
2014
Wydawca:
Instytut Ogrodnictwa
Tematy:
Lisianthus
Eustoma grandiflorum
peroxidase
8-hydroxyquinoline citrate
sucrose
superoxide dismutase
malondialdehyde
water uptake
protein
anthocyanin content
cut flower
quality parameter
vase life
Źródło:
Journal of Horticultural Research; 2014, 22, 1
2300-5009
Pojawia się w:
Journal of Horticultural Research
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Mikrofluidyka - technologia miniaturyzacji laboratorium
Autorzy:
Laskowski, M.
Powiązania:
https://bibliotekanauki.pl/articles/274256.pdf
Data publikacji:
2011
Wydawca:
Roble
Tematy:
mikrofluidyka
technologie mikrocieczowe
mikromacierze DNA
lab-on-a-chip
droplet-on-demand
peroksydaza
microfluidics
DNA microarrays
peroxidase
Źródło:
LAB Laboratoria, Aparatura, Badania; 2011, 16, 1; 41-43
1427-5619
Pojawia się w:
LAB Laboratoria, Aparatura, Badania
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Antioxidant defence in maize cultivars triggered by the two-spotted spider mite attack and soil drought – a comparative study
Autorzy:
Czapla, A.
Grudkowska, M.
Miazek, A.
Zagdanska, B.
Kielkiewicz, M.
Powiązania:
https://bibliotekanauki.pl/articles/80869.pdf
Data publikacji:
2013
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
conference
antioxidant defense
maize cultivar
two-spotted spider mite
Tetranychus urticae
soil drought
oxidative stress
ascorbate peroxidase
catalase
glutathione reductase
peroxidase
polyphenol oxidase
Źródło:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2013, 94, 2
0860-7796
Pojawia się w:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Wpływ zmian ciśnienia otoczenia na aktywność peroksydazy glutationowej (GPX) i atalazy (CAT) we krwi nurków : badania wstępne
The effect of ambient pressure changes on the activity of glutathione peroxidase (GPX) and catalase (CAT) in the blood divers : preliminary results
Autorzy:
Włodarski, A.
Woźniak, A.
Mila-Kierzenkowska, C.
Sutkowy, P.
Powiązania:
https://bibliotekanauki.pl/articles/366531.pdf
Data publikacji:
2013
Wydawca:
Polskie Towarzystwo Medycyny i Techniki Hiperbarycznej
Tematy:
ciśnienie
nurkowanie
peroksydaza glutationowa
katalaza
pressure
diving
glutathione peroxidase
catalase
Opis:
Zmiana warunków otoczenia jest stresem dla organizmu, który może prowadzić do wzmożonej generacji reaktywnych form tlenu i licznych uszkodzeń w obrębie komórek. Istotną rolę w przeciwdziałaniu tym uszkodzeniom pełnią enzymy antyoksydacyjne. Celem niniejszej pracy było określenie wpływu zmian ciśnienia otoczenia w warunkach nurkowania na aktywność peroksydazy glutationowej oraz katalazy. W badaniu wzięło udział 11 płetwonurków w wieku od 18 do 41 lat. Badani spędzili 40 min w jeziorze, w wodzie o temperaturze 13° C, schodząc maksymalnie na głębokość około 9 m. Podczas zanurzenia wszyscy oddychali powietrzem. Krew do badań pobrano z żyły odłokciowej dwukrotnie: przed zanurzeniem w wodzie (kontrola) oraz bezpośrednio po wynurzeniu. W pracy wykazano tendencję do wzrostu aktywności katalazy oraz tendencję do obniżania się aktywności peroksydazy glutationowej, ale zmiany te nie były istotne statystycznie. Ciśnienie otoczenia wywierane przez wodę podczas nurkowania nie ma istotnego wpływu na aktywność peroksydazy glutationowej oraz katalazy w erytrocytach. Może to sugerować, że nie doszło do zwiększonej generacji nadtlenku wodoru będącego substratem reakcji katalizowanych przez oba enzymy. Brak istotnych statystycznie zmian aktywności badanych enzymów antyoksydacyjnych może być efektem zmian przystosowawczych w organizmie osób nurkujących.
The changes in ambient conditions are usually stress factor for the organism, which may lead to increased generation of reactive oxygen species and numerous damages within the cells. The important role in counteracting of free radical damages to cells is played by antioxidant enzymes. The aim of this study was to determine the impact of changes in ambient pressure during diving on activity of selected antioxidant enzymes: glutathione peroxidase and catalase . The study involved eleven divers, aged from 18 to 41 years. Subjects spent 40 minutes in the lake, in water at 13° C, going down to a depth of about 9 m. All divers breathe with the air. Blood samples were taken from basilica vein twice: before immersion in water (control) and immediately after surfacing. The study showed an increasing trend in catalase activity and trend to decrease in glutathione peroxidase activity, but the changes were not statistically significant. Ambient pressure exerted by the water during diving has no significant effect on the activity of glutathione peroxidase and catalase in erythrocytes. This may suggest, that there was no increased generation of hydrogen peroxide which is a substrate of the reaction catalyzed by two enzymes. No statistically significant differences in the activity of antioxidant enzymes could be due to adaptive changes in the body of divers.
Źródło:
Polish Hyperbaric Research; 2013, 1(42); 7-25
1734-7009
2084-0535
Pojawia się w:
Polish Hyperbaric Research
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Antioxidant status in erythrocytes of cystic fibrosis children.
Autorzy:
Laskowska-Klita, Teresa
Chełchowska, Magdalena
Powiązania:
https://bibliotekanauki.pl/articles/1044199.pdf
Data publikacji:
2001
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
superoxide dismutase
catalase
glutathione peroxidase
vitamin E
erythrocytes
cystic fibrosis
Opis:
Activities of superoxide dismutase, catalase and glutathione peroxidase in erythrocytes of cystic fibrosis children were studied in order to estimate the severity of their deficiency. Our results point to increased susceptibility of erythrocytes of cystic fibrosis subjects to oxidative injury and indicate that the antioxidant status of patients should be carefully monitored.
Źródło:
Acta Biochimica Polonica; 2001, 48, 1; 283-285
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The activities of superoxide dismutase, catalase, glutathione peroxidase and glutathione redudase in erythrocytes of rats with experimental neoplastic disease
Autorzy:
Batko, Jolanta
Warchoł, Teresa
Karoń, Halina
Powiązania:
https://bibliotekanauki.pl/articles/1045161.pdf
Data publikacji:
1996
Wydawca:
Polskie Towarzystwo Biochemiczne
Źródło:
Acta Biochimica Polonica; 1996, 43, 2; 403-405
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The participation of peroxidase in adaptation of plants to moderate water deficit
Udział peroksydaz w adaptacji roślin do warunków umiarkowanego deficytu wody
Autorzy:
Nedukha, O.M.
Powiązania:
https://bibliotekanauki.pl/articles/797468.pdf
Data publikacji:
2006
Wydawca:
Szkoła Główna Gospodarstwa Wiejskiego w Warszawie. Wydawnictwo Szkoły Głównej Gospodarstwa Wiejskiego w Warszawie
Opis:
The aim of this investigation was to study the localization and activity of peroxidase (EC. 1.11.1.7) in the leaves of Sium latifolium L. and interaction of peroxidase activity with lignin synthesis at moderate water deficit. The leaves of two ecological forms (ecotypes) in flowering phase were studied. S. latifolium air- aquatic ecotype grew in water (on the bank of Psel River, Ukraine), and terrestrial ecotype plant grew along the bank of this river under the conditions of moderate water deficit. The immunocytochemical and biochemical methods were used for the investigation. Peroxidase activity was estimated in chloroplasts of both palisade and spongy mesophyll and in cell walls of epidermis and mesophyll. The adaptation of S. latifolium plants to the moderate water deficit leads to the increase of guaiacol- and ascorbate peroxidase activities and lignin content in leaves, and also to the appearance of peroxidase in vacuoles.
Zbadano lokalizację i aktywności peroksydaz (EC. 1.11.1.7) w liściach Sium latifolium oraz określono interakcję pomiędzy aktywnością enzymu i syntezą lignin w warunkach umiarkowanego deficytu wody. Badania przeprowadzono na dwóch ekotypach S. latifolium w fazie kwitnienia roślin. Uprawę ekotypu powietrzno-wodnego prowadzono w wodzie, na brzegu rzeki Psel na Ukrainie, a ekotypu lądowego wzdłuż brzegu rzeki w warunkach umiarkowanego deficytu wody. Zasosowano immunocytochemiczne i biochemiczne metody analizy aktywności peroksydazy i zawartości lignin. Aktywność peroksydazy oznaczono w chloroplastach miękiszu palisadowego i gąbczastego oraz w ścianach komórkowych epidermy i mezofilu. Adaptacja S. latifolium do waruków umiarkowanego deficytu wody wiązała się ze wzrostem aktywności enzymów i zawartością lignin, a także pojawieniem się peroksydaz w wakuolach liści ekotypu lądowego.
Źródło:
Zeszyty Problemowe Postępów Nauk Rolniczych; 2006, 509
0084-5477
Pojawia się w:
Zeszyty Problemowe Postępów Nauk Rolniczych
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Plant peroxidases and nitrogen metabolism: interactions with nitrates and nitrites
Autorzy:
Minibayeva, F.
Galeeva, E.
Gurjanov, O.
Viktorova, L.
Powiązania:
https://bibliotekanauki.pl/articles/80640.pdf
Data publikacji:
2013
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
conference
peroxidase
plant
nitrogen metabolism
oxidoreductase
nitrate
nitrite
Źródło:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2013, 94, 2
0860-7796
Pojawia się w:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Isolation and partial purification of a glutathione peroxidase from pig blood platelets
Izolowanie i częściowe oczyszczanie peroksydazy glutationowej z płytek krwi świni
Autorzy:
Żbikowska, Halina
Wachowicz, Barbara
Powiązania:
https://bibliotekanauki.pl/articles/945016.pdf
Data publikacji:
1996
Wydawca:
Uniwersytet Łódzki. Wydawnictwo Uniwersytetu Łódzkiego
Opis:
Metodą Maddipati i Marnett (1987) wyizolowano i częściowo scharakteryzowano peroksydazę glutationową z płytek krwi świni. Proces oczyszczania przebiegał kolejno poprzez wysalanie siarczanem amonu, hydrofobową chromatografię na złożu Phenyl-Sepharose CL-4B oraz chromatografię jonowymienną. Elektroforeza w żelu poliakryloamidowym z SDS (w obecności /3-merkaptoetanolu) enzymu otrzymanego w ostatnim etapie oczyszczania ujawniła obecność podjednostki o masie cząsteczkowej 23 kDa. Wykazano, że w płytkach dominującą formą GSH-Px jest selenozależna peroksydaza glutationowa, ktora wykazuje ok. 86% całkowitej aktywności tego enzymu. Selenin sodowy powodował wzrost aktywności otrzymanego enzymu, podczas gdy jony miedzi, NEM i cisplatyna była inhibitorami peroksydazy glutationowej.
Źródło:
Acta Universitatis Lodziensis. Folia Biochimica et Biophysica; 1996, 11
0208-614X
Pojawia się w:
Acta Universitatis Lodziensis. Folia Biochimica et Biophysica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Generation of superoxide anion and induction of superoxide dismutase and peroxidase in bean leaves infected with pathogenic fungi
Autorzy:
Urbanek, Henryk
Gajewska, Ewa
Karwowska, Renata
Wielanek, Marzena
Powiązania:
https://bibliotekanauki.pl/articles/1045088.pdf
Data publikacji:
1996
Wydawca:
Polskie Towarzystwo Biochemiczne
Źródło:
Acta Biochimica Polonica; 1996, 43, 4; 679-685
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Growth regulators and guaiacol peroxidase activity during the induction phase of somatic embryogenesis in Picea species
Autorzy:
Hazubska-Przybyl, T.
Ratajczak, E.
Kalemba, E.M.
Bojarczuk, K.
Powiązania:
https://bibliotekanauki.pl/articles/41387.pdf
Data publikacji:
2013
Wydawca:
Polska Akademia Nauk. Instytut Dendrologii PAN
Opis:
Biochemical studies during the induction phase of somatic embryogenesis in Picea abies [L.] Karst. and P. omorika [Pančić] Purk. can supplement our basic knowledge of the developmental processes accompanying the formation of embryogenic tissues from explants. Such studies may also contribute to finding the markers specific to the early stages of somatic embryogenesis of spruce species and, consequently, to the optimization of the process of initiation of embryogenic tissues from different types of plant explants treated with various growth regulator combinations. In this paper the effect of certain growth regulator systems on enzymatic activity was studied. The analysis of guaiacol peroxidase activity (EC 1.11.1.7), based on the spectrophotometric method, showed that this activity was the lowest in mature zygotic embryos (explants) and significantly higher in 8-week-old embryogenic and non-embryogenic calluses treated with various combinations of growth regulators. In the newly initiated embryogenic tissue, the activity of this enzyme decreased and remained at a lower level during proliferation, irrespective of the applied growth regulator combination. The type and concentration of growth regulators used for the initiation and proliferation of embryogenic tissues had no statistically significant effect on peroxidase activity, although during the initiation often its increased level was observed in calluses treated with 2.4-D. Detection of guaiacol peroxidase activity in the induction phase of somatic embryogenesis proves its participation in this process. The subsequent change in its activity indicates that this peroxidase can be a biochemical marker of somatic embryogenesis of the tested spruce species.
Źródło:
Dendrobiology; 2013, 69
1641-1307
Pojawia się w:
Dendrobiology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Redox state modulation in rice and its implications for plant development and stress responses
Autorzy:
Caverzan, A.
Ribeiro, C.
Lazzarotto, F.
Passaia, G.
Rosa, S.
Garighan, J.
Piovesana, M.
Carvalho, F.
Albenisio, J.
Margis, R.
Margis-Pinheiro, M.
Powiązania:
https://bibliotekanauki.pl/articles/79908.pdf
Data publikacji:
2013
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
conference
reactive oxygen species
scavenging enzyme
detoxification
redox state
rice
plant development
stress response
plant cell
biochemical property
antioxidative enzyme
ascorbate peroxidase
glutathione peroxidase
Źródło:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2013, 94, 2
0860-7796
Pojawia się w:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Class III peroxidases in maize plasma membranes
Autorzy:
Moller, B.
Luthje, S.
Powiązania:
https://bibliotekanauki.pl/articles/79851.pdf
Data publikacji:
2013
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
conference
class III peroxidase
maize
plasma membrane
isoenzyme
thylakoid membrane
tonoplast membrane
oxidative stress
Źródło:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2013, 94, 2
0860-7796
Pojawia się w:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Isolation and computational characterization of glutathione peroxidase gene from an aquatic fern - Salvinia molesta
Autorzy:
Rahaman, S.
Singh, P.K.
Basu, P.
Gupta, S.
Basu, M.
Ganguli, S.
Powiązania:
https://bibliotekanauki.pl/articles/11200.pdf
Data publikacji:
2016
Wydawca:
Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
Opis:
Pteridophytes and more specifically ferns represent a large but threatened group of plants which often serve as important environmental markers for pollution. Reports regarding stress responses in ferns are rare, apart from a few studies involving the ecological distribution and molecular marker studies. This work isolates a glutathione peroxidase enzyme from an aquatic fern widely distributed in fresh and polluted water bodies adjacent to sources of environmental polluted sources. Further computational analyses were performed to study the structure of the protein encoded by the open reading frame. Results indicate the presence of a large number of binding pockets which serve as important binding sites in the interactions with the cognate ligands.
Źródło:
International Letters of Natural Sciences; 2016, 51
2300-9675
Pojawia się w:
International Letters of Natural Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Purification of peroxidase from the medicinal herb Andrographis paniculata causing fungal hyphal distortion
Oczyszczanie peroksydazy z leczniczej rośliny Andrographis paniculata, powodującej zniekształcenia strzępek grzybni
Autorzy:
Ghosh, M.
Powiązania:
https://bibliotekanauki.pl/articles/71287.pdf
Data publikacji:
2010
Wydawca:
Instytut Włókien Naturalnych i Roślin Zielarskich
Opis:
Peroxidases are ubiquitous in plants and their pattern of expression depends on tissue, development stage and environmental stimuli. They are involved in numerous physiological functions, including defense against pathogens and insect pests. The present study was conducted to isolate and purify a constitutive leaf peroxidase from the medicinal herb, Andrographis paniculata. The enzyme was purified through a series of purification steps and a 35kDa peroxidase was purified to homogeneity with pI value of 6.0. In vitro hyphal extension inhibition assay against the forest pathogen Trichosporium vesiculosum revealed significant morphological distortion of the protein treated hyphae.
Peroksydazy są wszechobecne w roślinach. Forma ich ekspresji zależy od tkanki, stadium rozwojowego i wpływu środowiska. Są one związane z licznymi funkcjami fizjologicznymi, w tym z obroną przed patogenami i szkodnikami. Celem przedstawionej pracy było wyizolowanie i oczyszczenie peroksydazy z liści rośliny leczniczej Andrographis paniculata. Enzym był oczyszczany wieloetapowo i peroksydaza 35 kDa była oczyszczona jako jednorodna przy pI=6,0. W doświadczeniach prowadzonych in vitro metodą zahamowania wzrostu grzybni Trichosporium vesiculosum (patogen leśny) wykazano istotne zniekształcenia białek badanej grzybni.
Źródło:
Herba Polonica; 2010, 56, 2
0018-0599
Pojawia się w:
Herba Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Disintegration of sludge by a two-stage treatment with hydrogen peroxide and solid-state fermentation by Aspergillus oryzae CGMCC5992
Autorzy:
Guan, G.
Zhicai, Z.
Jun, J.
Fan, F.
Keping, C.
Powiązania:
https://bibliotekanauki.pl/articles/208076.pdf
Data publikacji:
2016
Wydawca:
Politechnika Wrocławska. Oficyna Wydawnicza Politechniki Wrocławskiej
Tematy:
Aspergillus oryzae CGMCC5992
fermentation
lignin peroxidase
manganese peroxidase
solid state fermentation
wastewater sludge
wastewater treatment plants
lignina peroksydazy
mangan peroksydazy
podłoże organiczne
fermentacja w stanie stałym
osady ściekowe
oczyszczalnie ścieków
Opis:
A novel and safe method has been reported for wastewater sludge treatment, the first step of which is H2O2 pretreatment at 150 cm3kg for 80 min. In this step, the majority of organic substrate was degraded, and the shear stress diminished significantly. In the second step, Aspergillus oryzae further decomposed the organic substrate of sludge for 8 days, and the activities of manganese peroxidase and lignin peroxidase kept increasing. By the methods, the residual chemical oxygen demand (COD) in the sludge was much lower than that in the sludge treated with high doses of H2O2. These results indicate that the present study provides a feasible method to safely dispose sludge from the wastewater treatment plant.
Źródło:
Environment Protection Engineering; 2016, 42, 3; 43-54
0324-8828
Pojawia się w:
Environment Protection Engineering
Dostawca treści:
Biblioteka Nauki
Artykuł
Wyświetlanie 1-50 z 149

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