Temat: secondary metabolites - Katalog OPAC zbiorów

Skocz do pozycji: 1.

Tytuł:: An improved method for RNA isolation from plants using commercial extraction kits
Autorzy:: Kalinowska, Elżbieta
Chodorska, Maria
Paduch-Cichal, Elżbieta
Mroczkowska, Karolina
Powiązania:: https://bibliotekanauki.pl/articles/1039717.pdf
Data publikacji:: 2012
Wydawca:: Polskie Towarzystwo Biochemiczne
Tematy:: total RNA
secondary metabolites
Opis:: Isolation of RNA from plants rich in secondary metabolites using commercial kits often results in contaminated preparations which are not suitable for downstream applications. Although many specific protocols appropriate for plants with a high content of phenolics, anthocyanins and polysaccharides have been developed, these are often expensive, time consuming and not applicable to different types of tissues. This study presents a simple and efficient modification of RNA extraction from different types of tissues using two commercial reagent kits. By simple improvement, we routinely obtained high-quality RNA of the following plants: the blackcurrant bush, black chokeberry bush, pear tree, apricot tree, apple tree, hardy kiwi, tangerine tree, highbush blueberry and cranberry plant.
Źródło:: Acta Biochimica Polonica; 2012, 59, 3; 391-393
0001-527X
Pojawia się w:: Acta Biochimica Polonica
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 2.

Tytuł:: Revealing and vetting of dynamic secondary metabolites position for isolation of antidermatophytic molecules from 20 aboriginal plants
Autorzy:: Singh, P. Shivakumar
Vidyasagar, G. M.
Powiązania:: https://bibliotekanauki.pl/articles/1076129.pdf
Data publikacji:: 2019
Wydawca:: Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
Tematy:: Aboriginal plants
antidermatophytic
dynamic secondary metabolites
phytochemical locations
vetting of secondary metabolites
Opis:: Aboriginal plants and their information clubbed with their potentiality. Majority of the secondary metabolites are the basic therapeutics. Usually these establishes in higher plants are gratifying all the time more noteworthy in drug scheming. In the current report, 100 different solvent extracts of 20 aboriginal plant species from Hyderabad Karnataka region were screened for their leading constituents of secondary metabolites. As of each one of plant species particular part of five successive extracts were particular for the revealing of impending metabolites. Intended for the vetting of secondary metabolites the criterion tests undertaken i.e., cluster wise for alkaloids dragendroff’s, tannin for ferric chloride, phenolics for lead acetate, glycoside for keller-killiani test, flavonoids for NaOH and saponins for foam test. The obvious ranges of secondary metabolites in the vein of non-polar to polar have been pragmatic. The utmost detection of alkaloids, tannins established at non-polar range whereas in middle polar flavonoids, tannins have been noticed. Glycosides and saponins entirely found at high polar. The upshot of the in attendance report will be very much constructive for isolation of diverse group of resulting metabolites in accumulate the time, chemicals, vigour utilization in active fragment drug design.
Źródło:: World Scientific News; 2019, 116; 25-35
2392-2192
Pojawia się w:: World Scientific News
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 3.

Tytuł:: Spectral fingerprinting revealed modulation of plant secondary metabolites in providing abiotic stress tolerance to invasive alien plants Lantana camara (L.), Parthenium hysterophorous (L.), Ricinus communis (L.), and Ageratum conyzoides (L.) (plant metabolites in stress tolerance to invasive plants)
Autorzy:: Sharma, A.D.
Kaur, J.
Chand, T.S.P.
Powiązania:: https://bibliotekanauki.pl/articles/2096781.pdf
Data publikacji:: 2021
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: chemical fingerprinting
invasiveness
lipid peroxidation
secondary metabolites
Opis:: Invasive alien species are non-native plant species that displace native species and pose adverse effects to environment, ecosystem, economy, and human health by diminishing the growth of native plants and by exhibiting higher stress tolerance. In our present study, four invasive alien species, namely Lantana camara, Parthenium hysterophorous, Ricinus communis, and Ageratum conyzoides, were studied from different locations. Plants growing under natural environmental conditions were sampled at random in the vicinity of Jalandhar. To gain insights into the biochemical basis of invasiveness of these plants, the samples were subjected to chemical fingerprinting by using UV-Vis, fluorescent, and Fourier transform-infrared (FT-IR) techniques under natural abiotic stress conditions (moderate and hot conditions). Indices of oxidative stress, such as malondialdehyde (MDA), were also studied. MDA levels were enhanced under hot conditions. Elevated peaks (major and minor) were observed in UV fingerprinting during adverse abiotic conditions. Fluorescent spectroscopy also validated the enhanced levels of secondary metabolites. FT-IR spectroscopy confirmed the presence of alkaloids and phenolics during stress conditions. Peaks were identified as rutin, vanillic acid, ascorbic acid, and glutathione reduced. The obtained results showed that under stressful conditions, the studied plants may produce an increased level of metabolites that might play a role in minimizing the oxidative stress faced by these plants. It was concluded that the studied plants, namely P. hysterophorus, L. camara, R. communis, and A. conyzoides, have the potential to cope with abiotic stress such as high temperature, which could be the reason for their invasiveness and vast adaptability.
Źródło:: BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2021, 102, 3; 307-319
0860-7796
Pojawia się w:: BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 4.

Tytuł:: Plant secondary metabolites as defenses: A review
Autorzy:: Jusuf, Herlina
Elveny, Marischa
Azizova, Feruza
Shichiyakh, Rustem A.
Kulikov, Dmitriy
Al-Taee, Muataz M.
Atiyah, Karrar K.
Jalil, Abduladheem T.
Aravindhan, Surendar
Powiązania:: https://bibliotekanauki.pl/articles/2174280.pdf
Data publikacji:: 2022
Wydawca:: Instytut Technologiczno-Przyrodniczy
Tematy:: alkaloids
flavonoids
phenolics
plant pathogens
secondary metabolites
Opis:: Plant secondary metabolites have a variety of functions, including mediating relationships between organisms, responding to environmental challenges, and protecting plants against infections, pests, and herbivores. In a similar way, through controlling plant metabolism, plant microbiomes take part in many of the aforementioned processes indirectly or directly. Researchers have discovered that plants may affect their microbiome by secreting a variety of metabolites, and that the microbiome could likewise affect the metabolome of the host plant. Pesticides are agrochemicals that are employed to safeguard humans and plants from numerous illnesses in urban green zones, public health initiatives, and agricultural fields. The careless use of chemical pesticides is destroying our ecology. As a result, it is necessary to investigate environmentally benign alternatives to pathogen management, such as plant-based metabolites. According to literature, plant metabolites have been shown to have the ability to battle plant pathogens. Phenolics, flavonoids, and alkaloids are a few of the secondary metabolites of plants that have been covered in this study.
Źródło:: Journal of Water and Land Development; 2022, 55; 206--211
1429-7426
2083-4535
Pojawia się w:: Journal of Water and Land Development
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 5.

Tytuł:: Impact of Water Stress and Temperature on Metabolites and Essential Oil of Rosmarinus officinalis (Phytochemical Screening, Extraction, and Gas Chromatography)
Autorzy:: Laftouhi, Abdelouahid
Eloutassi, Noureddine
Drioua, Soufiane
Ech-Chihbi, Elhachmia
Rais, Zakia
Abdellaoui, Abdelfattah
Taleb, Abdslam
Beniken, Mustapha
Taleb, Mustapha
Powiązania:: https://bibliotekanauki.pl/articles/24201782.pdf
Data publikacji:: 2023
Wydawca:: Polskie Towarzystwo Inżynierii Ekologicznej
Tematy:: climate change
primary metabolites
secondary metabolites
essential oil yield
Rosmarinus officinalis
Opis:: Currently, climate change is disrupting life on Earth by causing imbalances in the biosphere. This work aimed to evaluate the impact of climate change on the content of primary and secondary metabolites and the yield of essential oil of Rosmarinus officinalis. Thus, the results of the conducted experiment show that the content of primary metabolites decreased with increasing temperature and decreasing precipitation along the experiment(proteins from 7.61% to 7.14%, carbohydrates from 6.92% to 5.64%, fats from 1.48% to 1.29% and dietary fiber from 4.96% to 4.22% and mineral composition: Ca from 7.67% to 5.98%, Mg from 8.61% to 7.01%, Fe from 7.53% to 7.21% and Mn from 6.85% to 3.97%), and the content of secondary metabolites increased in the second year when increasing the temperature by 5 °C and water stress by 50% (coumarin from 6.59% to 10.99%, saponins from 7.15% to 8.46%, tannin from 3.92% to 5.95%, alkaloids from 6.69% to 15.62% and flavonoid from 8.02% to 15.75%),but in the fourth year when the temperature continued to increase and water stress was 75% the content of secondary metabolites decreased (coumarin from 10.99% to 8.27%, saponins from 8.46% to 7.87%, tannin from 5.95% to 4.85%, alkaloids from 15.62% to 10.68% and flavonoid from 15.75% to 11.36%)and the same results were obtained for the yield of essential oil which increased in the second year and decreased in the fourth year. This GC analysis of the three essential oil samples shows that the majority of compounds in the three essential oils studied are cineole (S1:45.98%, S2:55.36%, S3:43.08%) followed by camphor (S1:17.44%, S2:21.44%, S3:21.56%) and Alpha-pinene (S1:9.30%, S2:8.34%, S3: 9.17%) and other compounds in low percentage.
Źródło:: Journal of Ecological Engineering; 2023, 24, 5; 237--248
2299-8993
Pojawia się w:: Journal of Ecological Engineering
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 6.

Tytuł:: Betaxanthin Profiling in Beta vulgaris Leaves and Gymnocalycium mihanovichii Grafted Cacti: A Comprehensive Study
Autorzy:: Spórna-Kucab, Aneta
Tekieli, Anna
Knapek, Magdalena
Zużałek, Marta
Wybraniec, Sławomir
Powiązania:: https://bibliotekanauki.pl/articles/24024625.pdf
Data publikacji:: 2023-12-05
Wydawca:: Instytut Rozrodu Zwierząt i Badań Żywności Polskiej Akademii Nauk w Olsztynie
Tematy:: beet
betalains
colorants
grafted cactus
LC-MS
secondary metabolites
Opis:: This study was focused on the identification and quantification of betaxanthins using high-performance liquid chromatography with a diode array detector coupled to electrospray ionisation mass spectrometry (HPLC-DAD-ESI-MS) in leaves of various cultivars of Beta vulgaris (beet) and Gymnocalycium mihanovichii grafted cacti. In G. mihanovichii grafted cacti, four betaxanthins, namely histidine-Bx, histamine-Bx, serine-Bx, and proline-Bx, were tentatively identified in the yellow, orange, pink, and red varieties, with contents ranging from 0.09 to 1.55 mg/kg fresh weight (FW). Betaxanthins were not detected in the green cactus. Histidine-Bx was the prevailing betaxanthin compound in the majority of cultivars. Fifteen betaxanthins were successfully identified in the leaves of five B. vulgaris cultivars (cv.): Snow Ball, Boldor, Cylindra, Rhubarb, and Round Dark Red. Leaves of yellow beet (cv. Boldor) had the highest total betaxanthin content (20.4 mg/kg FW), while white beet (cv. Snow Ball) had the lowest one (3.43 mg/kg FW). The leaves of red cultivars had comparable betaxanthin contents, ranging from 13.4 to 18.8 mg/kg FW, similary to the yellow cultivar, indicating their potential as valuable sources of betaxanthins. There was no single dominant betaxanthin in B. vulgaris leaves. The leaves of B. vulgaris were found to be a richer source of betaxanthin than the grafted cactus G. mihanovichii, which opens up new prospects for research into the potential utilization of these compounds in the industry.
Źródło:: Polish Journal of Food and Nutrition Sciences; 2023, 73, 4; 375-384
1230-0322
2083-6007
Pojawia się w:: Polish Journal of Food and Nutrition Sciences
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 7.

Tytuł:: Total phenolic, flavonoid content and antioxidant capacity of stem bark, root, and leaves methanolic extract of Rhizophora mucronata Lam.
Autorzy:: Mulyani, Yeni
Syaputra, Nabil Dio
Dewi, Khoirunisa Candra
Lili, Walim
Agung, M. Untung Kurnia
Powiązania:: https://bibliotekanauki.pl/articles/1076655.pdf
Data publikacji:: 2019
Wydawca:: Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
Tematy:: Antioxidant
Flavonoid
Indramayu Regency
Phenolic
Rhizophora mucronata
Secondary metabolites
Opis:: The study was carried out to evaluate the phytochemical constituents, antioxidant activity and the content of total phenolic and flavonoid in the methanolic extract from stem bark, root, and leaves of Rhizophora mucronata from Karangsong, Indramayu Regency, West Java, Indonesia. The antioxidant activity was evaluated using 2,2-diphenyl-1-picrilhydrazyl (DPPH) assay. Total phenolic and flavonoid contents were determined according respectively to Folin-Ciocalteau method, and aluminium trichloride method. The methanol extract from stem bark of R. mucronata contained secondary metabolite such as phenolic, flavonoid, tannin and saponin. The stem bark of R. mucronata showed the highest phenolic and flavonoid content and possessed a higher antioxidant activity (IC50 84.80 µg·m/L) than that of root (IC50 166.95 µg·m/L) and leaves (IC50 90.51 µgmL1). Total phenolic and flavonoid content in the stem bark of R. mucronata were 131.91 ±1.7 mg gallic acid equivalents/g and 902 ±0.7 mg quercetin equivalents/g, respectively.
Źródło:: World News of Natural Sciences; 2019, 26; 118-127
2543-5426
Pojawia się w:: World News of Natural Sciences
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 8.

Tytuł:: The evaluation of the in-vitro antimicrobial activity of Alangium salvifolium (Linn)
Autorzy:: Khan, Mohammad Nadeem
Powiązania:: https://bibliotekanauki.pl/articles/1109620.pdf
Data publikacji:: 2018
Wydawca:: Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
Tematy:: Alangium salvifolium
Antimicrobial activity
disc diffusion
secondary metabolites
soxhelt apparatus
Opis:: Finding healing power in plants is an ancient idea, and people of all continents and civilization have been using plants in one form or the other for poultices or as decoctions. Alangium salvifolium (Linn), family Alangeaceae, is a tree that grows in the wild throughout India. The plant has been used in the Indian traditional system of medicine for skin diseases (e.g. leucoderma), articular diseases, and anti-inflammation, anti-poisonous, anti-pyretic, and anti-emetic requirements. However, no scientific evidence is available regarding its antimicrobial activity. An investigation of Alangium salvifolium as an antimicrobial activity agent is the objective of our present study. The ATCC culture used in this study was collected from department of Microbiology, G.M.C., Bhopal (M.P.). Shade dried crude powder (200 grams) of seed of Alangium salvifolium was separately extracted with methanol in a soxhelt apparatus. The Antimicrobial activities were then studied by applying the Disc–Diffusion method. Our results indicate that the observed antimicrobial activity of the fraction appears to be due to unknown secondary metabolites in it. H.P.L.C. (high performance liquid chromatography) and chemical studies may, thus be useful in analyzing the presence of unknown secondary metabolites in the fractions.
Źródło:: World News of Natural Sciences; 2018, 20; 176-181
2543-5426
Pojawia się w:: World News of Natural Sciences
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 9.

Tytuł:: Chemical diversity of silverweed (Potentilla anserina L.) growing at the edges of arable fields.
Autorzy:: Angielczyk, Mirosław
Bączek, Katarzyna
Geszprych, Anna
Przybył, Jarosław L.
Węglarz, Zenon
Powiązania:: https://bibliotekanauki.pl/articles/2199570.pdf
Data publikacji:: 2010-06-01
Wydawca:: Instytut Hodowli i Aklimatyzacji Roślin
Tematy:: catechins
flavonoids
phenolic acids
populations
secondary metabolites
silverweed herb
tannins
Opis:: The medicinal usage of silverweed herb (Anserinae herba) is primarily related to the presence of phenolic compounds. Three populations of silverweed (Potentilla anserina L.) occurring at the edges of arable fields in Podlasie area (north-eastern Poland) were compared in respect of the content and composition of phenolics. Herb collected from the investigated populations differed in the content of determined compounds. The tannin content was very low (0.81-0.91%). The total flavonoid content ranged from 0.48 to 0.60%. Phenolic acid content was much higher (1.38-2.26%). Three flavan-3-ols: (+)-catechin, (-)-epicatechin, and (-)-epigallocatechin, four flavonol glycosides: rutoside, quercetin-3-O-glucoside, isorhamnetin-3-O-glucoside, and kaempferol-3-O-glucoside, as well as one phenolic acid (ellagic acid) were identified as a_result of HPLC analysis of methanolic extracts from the investigated raw materials. The most considerable differences between populations concerned the content of (+)-catechin (87.0-199.3 mg × 100 g-1), (-)-epigallocatechin (141.9-290.6 mg × 100 g-1), and rutoside (192.3-386.0 mg × 100 g-1) in herb...
Źródło:: Plant Breeding and Seed Science; 2010, 61; 41-46
1429-3862
2083-599X
Pojawia się w:: Plant Breeding and Seed Science
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 10.

Tytuł:: Bioinformatic insight into Portulaca oleracea L. (Purslane) of Bulgarian and Greek origin
Autorzy:: Balabanova, V.
Hristov, I.
Zheleva-Dimitrova, D.
Sugareva, P.
Lozanov, V.
Gevrenova, R.
Powiązania:: https://bibliotekanauki.pl/articles/2117810.pdf
Data publikacji:: 2020
Wydawca:: Polska Akademia Nauk. Czasopisma i Monografie PAN
Tematy:: Portulaca oleracea
LC–HRAMS
secondary metabolites
descriptive analysis
multivariate statistics
Opis:: Portulaca oleracea L. (Portulacaceae) is used as functional food and its nutritional and therapeutic properties are related to the high levels of organic and fatty acids, polyphenols, polysaccharides and cyclo-dopa amides. This study presents a strategy based on liquid chromatography – high resolution accurate mass spectrometry method (LC – HRAMS) and bioinformatic methods to analyze 33 purslane accessions originating from 11 floristic regions in Bulgaria together with 5 accessions of Greek provenance. Extracts were obtained by microwave extraction. Based on the LC-MS metabolic “fingerprints” of assayed samples, a purslane metabolic database was developed. LC-MS data were proceeded with Software application Compound Discover 2.0 (Thermo Fischer Sci., USA). Principal Component Analysis (PCA) combined with both descriptive and differential analyses were used to find marker metabolites to distinguish different geographical regions. The differential analysis of the Bulgarian and Greek samples allowed the identification of 50 marker metabolites. Based on accurate masses, retention times, fragmentation patterns in MS/MS, comparison with commercial standards and literature data, these secondary metabolites were identified after detailed analysis of Volcano-plots. For the first time, 29 compounds are reported. The identified compounds were used to perform a study of the biosynthetic pathways of purslane secondary metabolites using Kyoto Encyclopedia of Genes and Genomes (KEGG) software platform. The statistical treatments identified marker compounds that can be used to distinguish the origin of accession set. Combining LC-MS data with multivariate statistical analysis was shown to be effective in studying the purslane metabolites, allowing for integration of chemistry with geographic origin.
Źródło:: Acta Biologica Cracoviensia. Series Botanica; 2020, 62, 1; 7-21
0001-5296
Pojawia się w:: Acta Biologica Cracoviensia. Series Botanica
Dostawca treści:: Biblioteka Nauki

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