Total phenolic, flavonoid content and antioxidant capacity of stem bark, root, and leaves methanolic extract of Rhizophora mucronata Lam.

The study was carried out to evaluate the phytochemical constituents, antioxidant activity and the content of total phenolic and flavonoid in the methanolic extract from stem bark, root, and leaves of Rhizophora mucronata from Karangsong, Indramayu Regency, West Java, Indonesia. The antioxidant activity was evaluated using 2,2-diphenyl-1-picrilhydrazyl (DPPH) assay. Total phenolic and flavonoid contents were determined according respectively to Folin-Ciocalteau method, and aluminium trichloride method. The methanol extract from stem bark of R. mucronata contained secondary metabolite such as phenolic, flavonoid, tannin and saponin. The stem bark of R. mucronata showed the highest phenolic and flavonoid content and possessed a higher antioxidant activity (IC50 84.80 µg·m/L) than that of root (IC50 166.95 µg·m/L) and leaves (IC50 90.51 µgmL1). Total phenolic and flavonoid content in the stem bark of R. mucronata were 131.91 ±1.7 mg gallic acid equivalents/g and 902 ±0.7 mg quercetin equivalents/g, respectively.