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    Wyświetlanie 1-92 z 92
    Tytuł:
    Application of gel filtration for rapeseed peptides analysis. Short report
    Autorzy:
    Amarowicz, R.
    Kmita-Glazewska, H.
    Powiązania:
    https://bibliotekanauki.pl/articles/1372750.pdf
    Data publikacji:
    1995
    Wydawca:
    Instytut Rozrodu Zwierząt i Badań Żywności Polskiej Akademii Nauk w Olsztynie
    Tematy:
    gel filtration
    peptide fraction
    peptide separation
    peptide
    peptide extraction
    food
    chromatographic analysis
    rapeseed
    Źródło:
    Polish Journal of Food and Nutrition Sciences; 1995, 04, 1; 88-92
    1230-0322
    2083-6007
    Pojawia się w:
    Polish Journal of Food and Nutrition Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Biologically active peptides derived from food proteins
    Autorzy:
    Kostyra, H.
    Kostyra, E.
    Powiązania:
    https://bibliotekanauki.pl/articles/1371450.pdf
    Data publikacji:
    1992
    Wydawca:
    Instytut Rozrodu Zwierząt i Badań Żywności Polskiej Akademii Nauk w Olsztynie
    Tematy:
    man
    peptide
    biologically active peptide
    natural peptide
    human nutrition
    nutrition
    food
    food protein
    protein degradation
    biotechnology
    Źródło:
    Polish Journal of Food and Nutrition Sciences; 1992, 01, 4; 5-17
    1230-0322
    2083-6007
    Pojawia się w:
    Polish Journal of Food and Nutrition Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    The search for new biological activities for selected insect peptides
    Autorzy:
    Kuczer, M.
    Dziubasik, K.
    Luczak, M.
    Majewska, A.
    Kamysz, W.
    Saniewska, A.
    Konopinska, D.
    Powiązania:
    https://bibliotekanauki.pl/articles/55140.pdf
    Data publikacji:
    2008
    Wydawca:
    Sieć Badawcza Łukasiewicz - Instytut Przemysłu Organicznego
    Tematy:
    antitumour peptide
    antifungal activity
    antiviral peptide
    oostatic peptide
    antiviral activity
    Phoma narcissi
    Botrytis tulipae
    colony growth
    biological property
    antimicrobial activity
    insect peptide
    biological activity
    insect
    peptide
    Opis:
    New biological properties of selected insect peptides are presented. The subjects of the investigation included insect oostatic peptides, like Neb-colloostatin (I) and Neb-TMOF(II), and/or insect peptides with antiviral or antitumor activity, such as alloferon (III) and its analogues modified at position 1 of the peptide chain. In the study was also included the oligopeptide Any-GS (VII) and its truncated analogues. The peptides were tested for antimicrobial activity on a series of bacterial species, antiviral activity against Human Herpes Virus type 1 (HHV-1) in vitro using a Vero cell line, and the growth and development of plant pathogens Phoma narcissi and Botrytis tulipae. The results of the biological investigations indicate that among the peptides investigated, compounds VII and IX inhibit the growth of plant pathogens P. narcissi and B. tulipae, whereas compounds I and II stimulate the mycelium growth of the aforementioned pathogens. Other peptides show slow antimicrobial activity but do not inhibit the replication of HHV-1 in Vero cells.
    Źródło:
    Pestycydy; 2008, 1-2; 5-11
    0208-8703
    Pojawia się w:
    Pestycydy
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Structural biology of the influenza virus fusion peptide
    Autorzy:
    Worch, Remigiusz
    Powiązania:
    https://bibliotekanauki.pl/articles/1039239.pdf
    Data publikacji:
    2014
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    influenza virus
    viral entry
    membrane fusion
    fusion peptide
    peptide-lipid interactions
    Opis:
    The release of influenza RNA inside the host cell occurs through the fusion of two membranes, the viral envelope and that of the cellular endosome. The fusion is mediated by the influenza hemagglutinin protein (HA), in particular by the fusion peptide (HAfp) located in the N-terminal fragment of HA2 subunit. This protein fragment anchors in the internal endosomal membrane, whereas the C-terminal HA2 part comprises a transmembrane domain (TMD) embedded in the viral envelope. A drop of pH in the endosome acts as the main trigger for HA2 large conformational change that leads to anchoring of the fusion peptide, close contact of the membranes and the subsequent fusion. Throughout the years the major research effort was focused on a 20-aminoacid fragment (HAfp1-20), shown by NMR to adopt a 'boomerang'-like structure. However, recent studies showed that extending HAfp1-20 by three highly conserved residues W21-Y22-G23 leads to formation of a unique, tight helical hairpin structure. This review summarizes recently discovered structural aspects of influenza fusion peptides and their relations with the membrane fusion mechanism.
    Źródło:
    Acta Biochimica Polonica; 2014, 61, 3; 421-426
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    PRRT as an alternative method of treatment in patient with glucagonoma syndrome: A case report
    Autorzy:
    Popławska-Kita, A.
    Szyszkowska, A.
    Brelska, P.
    Kowalczuk, M.
    Szostek, A.
    Popławski, Ł.
    Siewko, K.
    Szelachowska, M.
    Kamiński, G.
    Werel, D.
    Górska, M.
    Powiązania:
    https://bibliotekanauki.pl/articles/1917699.pdf
    Data publikacji:
    2016
    Wydawca:
    Uniwersytet Medyczny w Białymstoku
    Tematy:
    Glucagonoma
    tumor
    peptide receptor radionuclide therapy
    Opis:
    Introduction: Glucagonoma is a rare pancreatic neuroendocrine tumor derived from alpha-cells of the islet of Langerhans. Due to oversecretion of glucagon it is associated with a characteristic paraneoplastic phenomenon, called glucagonoma syndrome, which consists of necrolytic migratory erythema (NME), weight loss, diabetes mellitus, diarrhea, normochromic normocytic anemia, deep vein thrombosis or pulmonary embolism and neuropsychiatric disturbances. Treatment modalities include surgical removal of tumor, somatostatin analogs and peptide receptor radionuclide therapy (PRRT). Case report: We present a case of 61-year-old woman diagnosed with glucagonoma in April 2012. Initially, body-caudal pancreatomy and resection of regional lymph nodes were performed. Five months after surgery, a PET-CT scan detected pathological mass with expression of somatostatin receptors in pancreatic body and metastases to regional lymph nodes. What is more, since April 2014 the patient had complained about persistent pruritus of the entire body. At present, due to the nonsurgical pancreatic mass and metastases she is treated with somatostatin analogs and PRRT. During this therapy the pruritus had decreased and currently there is no sign of cutaneous disease. Moreover, reduction of tumor size was obtained. Conclusions: PRRT may reduce tumor size and by reducing bothersome symptoms substantially improve the quality of life in patients with SSTR-positive tumors
    Źródło:
    Progress in Health Sciences; 2016, 6(1); 209-214
    2083-1617
    Pojawia się w:
    Progress in Health Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Biological evaluation of analogues of an insect neuropeptide proctolin.
    Autorzy:
    Woźnica, Iwona
    Szeszel-Fedorowicz, Wioletta
    Rosiński, Grzegorz
    Konopińska, Danuta
    Powiązania:
    https://bibliotekanauki.pl/articles/1043325.pdf
    Data publikacji:
    2004
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    proctolin analogues
    proctolin
    insect myotropic peptide proctolin
    Opis:
    Continuing our studies on proctolin (Arg-Tyr-Leu-Pro-Thr) we performed the synthesis and biological evaluation of 52 analogues substituted in position 2, 3, 4, and 5 of the peptide chain. The peptides were bioassayed for cardiotropic activity in vitro on Tenebrio molitor and myotropic activity on foregut of Schistocerca gregaria. Twenty analogues retained 20-80% of proctolin activity.
    Źródło:
    Acta Biochimica Polonica; 2004, 51, 1; 115-119
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Antibacterial peptides of the moth Galleria mellonella
    Autorzy:
    Mak, Paweł
    Chmiel, Dorota
    Gacek, Grzegorz
    Powiązania:
    https://bibliotekanauki.pl/articles/1044082.pdf
    Data publikacji:
    2001
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    antibacterial peptide
    bacteriocin
    insect immunity
    hemolymph
    cecropin
    Opis:
    The work describes purification and biochemical characterization of two inducible antimicrobial peptides from the hemolymph of Galleria mellonella. The peptides were isolated by a sequence of reversed-phase chromatography steps from the hemolymph of larvae immunized with viable bacteria. The first peptide is a member of the cecropin family while the second one is rich in proline residues and has a unique sequence.
    Źródło:
    Acta Biochimica Polonica; 2001, 48, 4; 1191-1195
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Chelating ability of proctolin tetrazole analogue
    Autorzy:
    Łodyga-Chruścińska, Elżbieta
    Sanna, Daniele
    Micera, Giovanni
    Chruściński, Longin
    Olejnik, Jadwiga
    Nachman, Ronald
    Zabrocki, Janusz
    Powiązania:
    https://bibliotekanauki.pl/articles/1041268.pdf
    Data publikacji:
    2006
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    metallopeptides
    metal complexes
    proctolin
    tetrazole peptide analogue
    Opis:
    The aim of the investigation was to establish the chelating ability of a new proctolin analogue of the sequence Arg-Tyr-LeuΨ[CN4]Ala-Thr towards copper(II) ions. The insertion of the tetrazole moiety into the peptide sequence has considerably changed the coordination ability of the ligand. Potentiometric and spectroscopic (UV-Vis, CD, EPR) results indicate that the incorporation of 1,5-disubstituted tetrazole ring favours the formation of a stable complex form of CuH-1L. This 4N coordination type complex is the dominant species in the physiological pH range. The tetrazole moiety provides one of these nitrogens. The data indicate that Cu(II) ions are strongly trapped inside the peptide backbone. These findings suggest that Cu(II) can hold peptide chains in a bent conformation. This bent conformation may be essential for bioactivity of the tetrazole peptides.
    Źródło:
    Acta Biochimica Polonica; 2006, 53, 1; 65-72
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Gene expression and peptide localization for LH-hCG receptor in porcine small and large luteal cells: possible regulation by opioid peptides
    Autorzy:
    Kaminski, T.
    Gawronska, B.
    Derecka, K.
    Okrasa, S.
    Przala, J.
    Powiązania:
    https://bibliotekanauki.pl/articles/69878.pdf
    Data publikacji:
    2000
    Wydawca:
    Polskie Towarzystwo Fizjologiczne
    Tematy:
    reverse transcription
    opioid peptide
    gene expression
    immunocytochemistry
    polymerase chain reaction
    peptide localization
    porcine luteal cell
    Źródło:
    Journal of Physiology and Pharmacology; 2000, 51, 2
    0867-5910
    Pojawia się w:
    Journal of Physiology and Pharmacology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Chicken meat proteins as potential precursors of bioactive peptides
    Autorzy:
    Dziuba, J.
    Minkiewicz, P.
    Plitnik, K.
    Powiązania:
    https://bibliotekanauki.pl/articles/1371527.pdf
    Data publikacji:
    1996
    Wydawca:
    Instytut Rozrodu Zwierząt i Badań Żywności Polskiej Akademii Nauk w Olsztynie
    Tematy:
    peptide
    proteolysis
    food biochemistry
    tropomyosin
    Gallus gallus
    collagen
    meat protein
    chicken meat
    myosin
    troponin
    bioactive peptide
    protein
    chicken
    Opis:
    Amino acid sequences of chicken (Gallus gallus) meat proteins: myosin, tropomyosin, troponin, collagen and connectin taken from SWISS-PROT and EMBL databases have been analysed using "PROTEIN" computer program searching for fragments identical to bioactive peptides and for bonds susceptible to the action of endopeptidases in protein chains. Chicken meat proteins contain fragments with antihypertensive (connectin), immunomodulating (myosin, tropomyosin, collagen), antithrombotic (collagen), antibacterial (collagen), embryotoxic (collagen) activity and also neuroactive (myosin, collagen, connectin) occurring in amino acid sequences with the frequency higher than that expected from the probability of appearance of given fragments in random amino acid sequences. There is a theoretical possibility of release of bioactive fragments from chicken meat proteins by endopeptidases. Such possibility especially occurs in the case of hydrolysis by proteinase K (EC 3.4.21.14). The frequency of occurrence of bioactive fragments may be applied for quantitative comparison of value of proteins as a source of bioactive peptides, although different affinity of bioactive fragments to their receptors and different susceptibility of proteins to proteolysis should be taken into consideration.
    Sekwencje aminokwasowe białek mięsa kurcząt (Gallus gallus): miozyny, tropomiozyny, troponiny, kolagenu i konektyny pochodzące z baz danych SWISS-PROT i E MB L były analizowane za pomocą programu komputerowego PROTEIN wyszukującego w łańcuchach białek fragmenty identyczne z sekwencjami bioaktywnych peptydów oraz wiązania podatne na działanie endopeptydaz. Białka mięsa kurcząt zawierają fragmenty o aktywności przeciwnadciśnieniowej (konektyna) (rys. 3), immunomodulacyjnej (miozyna, tropomiozyna, kolagen), przeciwkrzepliwej (kolagen), antybakteryjnej (kolagen), embriotoksycznej (kolagen) (rys. 2) oraz neuroaktywne (miozyna, kolagen, konektyna) (rys. 2, 3) występujące w sekwencjach aminokwasowych z częstością większą, niż przewidywana na podstawie prawdopodobieństwa pojawienia się danych fragmentów w przypadkowych sekwencjach aminokwasowych (rys. 1). Istnieje teoretyczna możliwość uwalniania bioaktywnych peptydów z białek mięsa kurcząt przez endopeptydazy. Taka możliwość istnieje szczególnie w przypadku hydrolizy przez proteinazę K (EC 3.4.21.14) (rys. 2 i 3). Częstość występowania bioaktywnych fragmentów może służyć do ilościowego porównywania potencjalnej wartości białek jako źródła bioaktywnych peptydów, chociaż musi być uwzględnione także różne powinowactwo fragmentów do ich receptorów oraz różna podatność białek na proteolizę.
    Źródło:
    Polish Journal of Food and Nutrition Sciences; 1996, 05, 4; 85-96
    1230-0322
    2083-6007
    Pojawia się w:
    Polish Journal of Food and Nutrition Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Cathelicidin LL-37: LPS-neutralizing, pleiotropic peptide
    Autorzy:
    Golec, M
    Powiązania:
    https://bibliotekanauki.pl/articles/51492.pdf
    Data publikacji:
    2007
    Wydawca:
    Instytut Medycyny Wsi
    Tematy:
    human organism
    human disease
    asthma
    lipopolysaccharide
    peptide
    pathogenic microorganism
    organic dust
    cathelicidin
    antimicrobial peptide
    dust
    endotoxin
    Opis:
    Human organism, constantly exposed to a large variety of pathogenic microorganisms and their products, such as lipopolysaccharide (LPS), developed innate immunity as a fi rst line of defence. One of the compartments of our organism well equipped with these defence mechanisms is the respiratory system. The cells lining the airways respond to the presence of virulent microorganisms by producing natural antimicrobial peptides, including the only member of the cathelicidins family found to date in humans, peptide LL-37. LL-37 is a small peptide of 37 amino acid residues. The peptide, in addition to its bactericidal effect, plays numerous roles in infl ammatory and tissue remodelling processes. It stimulates angiogenesis, induces proliferation of lung epithelial cells, accelerates wound closure of the airway epithelium, and provokes cytokine release (e.g. IL-8) and cell migration. LL-37 is also able to neutralize LPS, a heteropolymer associated with organic dust, produced by Gram-negative bacteria. LPS (commonly referred to as endotoxin) plays an important role in pathogenesis of many respiratory diseases caused by organic dust, including organic dust toxic syndrome and chronic illnesses such as chronic obstructive pulmonary disease (COPD), asthma or allergic alveolitis (hypersensitivity pneumonitis). LPS is a strong pro-infl ammatory stimulus, inducing in respiratory airways expression of antimicrobial peptides, including LL-37, which is in turn a potent LPS-neutralizing factor. The article discusses the complex interplay between endotoxin and the LPS-neutralizing, pleiotropic peptide LL-37 in pathogenic mechanisms of lung diseases, with regard to closer perspectives of using LL-37 and its derivatives as therapeutic agents.
    Źródło:
    Annals of Agricultural and Environmental Medicine; 2007, 14, 1
    1232-1966
    Pojawia się w:
    Annals of Agricultural and Environmental Medicine
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Detekcja enzymów z wykorzystaniem macierzy polimer-substrat
    Enzyme assays based on polymer-substrate arrays
    Autorzy:
    Trzcińska, R.
    Utrata-Wesołek, A.
    Suder, P.
    Dworak, A.
    Silberring, J.
    Trzebicka, B.
    Powiązania:
    https://bibliotekanauki.pl/articles/285196.pdf
    Data publikacji:
    2011
    Wydawca:
    Akademia Górniczo-Hutnicza im. Stanisława Staszica w Krakowie. Polskie Towarzystwo Biominerałów
    Tematy:
    macierze peptydowe
    koniugaty polimer-peptyd
    enzymy
    powierzchnie
    peptydy
    peptide arrays
    conjugates polymer-peptide
    enzymes
    surfaces
    peptides
    Opis:
    Ocena aktywności enzymów jest kluczowym zagadnieniem w badaniu kinetyki reakcji enzymatycznych i inhibicji. Ostatnio, macierze polimerowo-peptydowe, gdzie peptyd jest specyficznym substratem dla enzymu wzbudzają coraz większe zainteresowanie wśród biochemików. W połączeniu z czułymi metodami detekcji takimi jak spektrometria mas i fluorescencja macierze mogą znaleźć zastosowanie jako niezwykle użyteczne narzędzia do detekcji enzymów i oceny ich aktywności w diagnostyce klinicznej. Macierze peptydowe składają się z powierzchni, na której immobilizowane są peptydy . Peptydy mogą być przyłączone bezpośrednio do sfunkcjonalizowanej powierzchni lub poprzez polimerowy linker. Obecność polimerowego linkera pomiędzy powierzchnią a peptydem przynosi wiele korzyści. Zastosowanie linkera z poli(tlenku etylenu) minimalizuje niespecyficzną adsorpcję biomolekuł na podłożu, zapewnia niskie tło w pomiarach fluorescencyjnych i ułatwia dostęp steryczny centrum aktywnego do peptydu. W niniejszej pracy koniugaty peptydów z poli(tlenkiem etylenu) zostały otrzymane na drodze syntezy na nośniku stałym metodą Fmoc. Otrzymane koniugaty immobilizowano na powierzchni krzemu zmodyfikowanej aminopropy lotrietoksy silanem. Powierzchnię scharakteryzowano techniką AFM i zbadano jej kąt zwilżania. Tak otrzymane macierze peptydowe poddano inkubacji z enzymami a produkty reakcji analizowano za pomocą techniki ESI MS.
    Enzyme assays are methods for measuring enzymatic activity, they are vital to study enzyme kinetics and enzyme inhibitors. Recently, polymer-peptide arrays where the peptide is specific substrate for the enzyme have attracted high attention. Such arrays can be applied as invaluable tools for enzyme recognition when connected to fluorescence or mass spectrometry detection. Peptide arrays consist of peptides immobilized on solid support directly or through polymeric linker. It w as shown that the presence of poly(ethylene oxide) as a linker between surface and the peptide offers many advantages. It minimizes non-specific binding of biomolecules on the surface, provides low background in fluorescence measurement, avoids steric hindrance , and thus makes linked peptide fully accessible the active site of an enzyme. In this work, conjugate of peptide and poly(ethylene oxide) were synthesized on the Tentagel resin using solid phase Fmoc chemistry, and then covalently immobilized by grafting-to method on the silica surface. Silica plates before grafting were modified by 3-aminopropy ltriethoxy silane and characterized by AFM and contact angel measurement. The obtained peptide arrays were incubated with enzymes and products of these reactions were analyzed by ESI MS.
    Źródło:
    Engineering of Biomaterials; 2011, 14, no. 109-111 spec. iss.; 36
    1429-7248
    Pojawia się w:
    Engineering of Biomaterials
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Use of o-phosphoserine [OPS] for the separation of peptides on immobilized copper ions
    Wykorzystanie OPS w procesie rozdzialu peptydow na unieruchomionych jonach miedzi
    Autorzy:
    Karas, M
    Powiązania:
    https://bibliotekanauki.pl/articles/14435.pdf
    Data publikacji:
    2010
    Wydawca:
    Uniwersytet Warmińsko-Mazurski w Olsztynie / Polskie Towarzystwo Magnezologiczne im. Prof. Juliana Aleksandrowicza
    Tematy:
    o-phosphoserine
    separation
    peptide
    immobilized copper ion
    copper ion
    metal ion
    protein
    diet component
    bioactive peptide
    Opis:
    Recent research into the structure and properties of proteins and peptides as physiologically active diet components has spurred a new interest in the isolation and investigation of bioactive peptides of animal, plant and microbiological origin. The isolation and separation of protein and peptide mixtures requires advanced procedures. It usually involves a multi-stage separation process on chromatographic columns with various packing. Immo- bilised Metal Ion Affinity Chromatography (IMAC) is frequently used in the complex process of obtaining peptide fractions. Affinity Chromatography (IMAC) relies on the specific interactions between amino acids, their reactive groups in proteins and peptides and „transitory” metal ions, in particular Cu2+. Those ions are immobilised by the chelating compound on the bed, forming specific adsorbents which bind proteins and peptides. The aim of this study was to determine whether o-phosphoserine (OPS) can be used for the immobilization of copper ions on Sephadex G25 during the separation of peptides and proteins isolated from string beans. Frozen pods of dwarf, green-podded string bean cv. Fana were used in the study. Peptide were extracted from well-homogenized string bean pods with tris-HCl buffer (pH 7.5), from which high molecular weight proteins were isolated with methanol, acetone, 20% trichloroacetic acid and the Magnafloc M-22S cation flocculant. The protein and peptide content of the separated fractions was determined. The peptide content depended on the type of extract from which high molecular weight proteins were isolated. The results obtained by using OPS as a chelating agent in the separation of string bean can be recommended for analysis of plant peptides.
    Rozwój nauki o strukturze oraz właściwościach białek i peptydów jako fizjologicznie aktywnych składnikach diety przyczynił się do wzrostu zainteresowania izolowaniem i badaniem bioaktywnych peptydów pochodzenia zwierzęcego, roślinnego i mikrobiologicznego. Izolowanie i rozdział mieszanin białek i peptydów wymaga zaawansowanej procedury. Stosuje się zazwyczaj kilkustopniowy rozdział na kolumnach chromatograficznych z różnym wypełnieniem. W tak skomplikowanym procesie otrzymywania frakcji peptydowych szerokie zastosowanie znalazła chromatografia powinowactwa na unieruchomionych jonach metali IMAC (Immobilized Metal Ion Affinity Chromatography). Chromatografia powinowactwa wykorzystuje specyficzne oddziaływania między aminokwasami oraz ich reaktywnymi ugrupowaniami w białkach i peptydach a jonami metali „przejściowych”, szczególnie zaś z Cu2+. Jony te są immobilizowane przez związek chelatujący na złożu, i w ten sposób stanowią specyficzne adsorbenty wiążące białka lub peptydy. Celem pracy było zbadanie przydatności OPS (o-fosfoseryny) jako czynnika unieruchamiającego jony miedzi na złożu w procesie rozdziału peptydów i białek wyizolowanych z fasoli szparagowej metodą IMAC. Materiałem do badań były mrożone strąki fasoli szparagowej karłowatej zielonostrąkowej, odmiany Fana. Peptydy i białka izolowano z fasoli szparagowej buforem Tris-HCl, z otrzymanego ekstraktu białka wysokocząsteczkowe wydzielono: metanolem, acetonem, 20% kwasem trichlorooctowym i kationowym flokulantem Magnafloc M-22S. W otrzymanych frakcjach oznaczono zawartość białka i peptydów. Peptydy obecne w fasoli szparagowej charakteryzowały się zbliżonym powinowactwem do jonów miedzi. Wykazano, że rozdział peptydów zależy w małym stopniu od właściwości czynnika zastosowanego podczas usuwania białek z ekstraktu. Przebieg rozdziału z wykorzystaniem OPS jako czynnika chelatującego w technice IMAC z powodzeniem może być stosowany do rozdziału peptydów z ekstraktów roślinnych.
    Źródło:
    Journal of Elementology; 2010, 15, 1; 101-110
    1644-2296
    Pojawia się w:
    Journal of Elementology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Antinociceptive effect of MAS MT in rats
    Autorzy:
    Rykaczewska-Czerwinska, M.
    Radosz, A.
    Szymanowska-Dziubasik, K.
    Konopinska, D.
    Plech, A.
    Powiązania:
    https://bibliotekanauki.pl/articles/55342.pdf
    Data publikacji:
    2008
    Wydawca:
    Sieć Badawcza Łukasiewicz - Instytut Przemysłu Organicznego
    Tematy:
    Tenebrio molitor
    antinociceptive effect
    rat
    larva
    brain
    neuropeptide
    insect neuropeptide
    Manduca sexta
    proctolin
    myotropin
    biological activity
    peptide chain
    peptide
    Opis:
    MAS MT is a myotropic decapeptide isolated from Manduca sexta. This peptide exerts stimulatory effect on insects heart-beat frequency. The present study was undertaken in order to determine a probable antinociceptive effect in rats of native synthetic decapeptide, MAS MT-I and its two analogs, heptapeptides MAS MT-II and MAS MT-III. All these peptides were applied directly into the lateral brain ventricle (icv) at three doses: 10, 25 and 50 nmol. The analgesic (antinociceptive) effect was evaluated by a tail immersion test. It was found that two doses of MAS MT-I: 25 and 50 nmol induced significant antinociceptive effect, while MAS MT-II and MAS MT-III exert a less antinociceptive effect in comparison with native MAS MT-I. Prior icv administration of naloxone, an opioid antagonist weakly blocked MAS MT-I effect. We conclude that antinociceptive effect of MAS MT-I in rats is not mediated by central opioid system.
    Źródło:
    Pestycydy; 2008, 3-4; 139-146
    0208-8703
    Pojawia się w:
    Pestycydy
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Synthesis and application of chiral triazine condensing reagents prepared from esters of amino acids.
    Autorzy:
    Kamiński, Zbigniew
    Zając, Krzysztof
    Jastrząbek, Konrad
    Powiązania:
    https://bibliotekanauki.pl/articles/1044064.pdf
    Data publikacji:
    2001
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    enantioselective reagent
    coupling reagent
    chiral triazines
    peptide synthesis
    Opis:
    Treatment of cyanuric chloride with chiral amines or esters of chiral amino acids gave chiral 2,4-dichloro-6-alkylamino-1,3,5-triazines (2-5) in 49-69% yield, which were found useful as coupling reagents. Enantioselective activation and enantioselective aminolysis in the presence of 2-5 was observed.
    Źródło:
    Acta Biochimica Polonica; 2001, 48, 4; 1143-1146
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Supported liquid membrane extraction of peptides.
    Autorzy:
    Drapała, Anna
    Dżygiel, Paweł
    Jönsson, Jan
    Wieczorek, Piotr
    Powiązania:
    https://bibliotekanauki.pl/articles/1044056.pdf
    Data publikacji:
    2001
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    Aliquat 336
    peptide
    extraction
    supported liquid membrane
    Opis:
    The application of supported liquid membrane (SLM) extraction for the enrichment of short peptides is presented. The extraction efficiency is dependent on the pH of donor phase and salt concentration in acceptor phase. Moreover, the extraction efficiency is also influenced by the peptide amino-acid sequence and hydrophobicity.
    Źródło:
    Acta Biochimica Polonica; 2001, 48, 4; 1113-1116
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Eliminating side effects of pain therapies
    Autorzy:
    Różycki, Krzysztof
    Rapacz, Jacek
    Fichna, Jakub
    Kosson, Piotr
    Powiązania:
    https://bibliotekanauki.pl/articles/1177402.pdf
    Data publikacji:
    2018
    Wydawca:
    Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
    Tematy:
    PAMORA
    cancer
    constipation
    morphine
    opioid
    pain
    peptide
    Opis:
    Pain is a very serious problem associated with many types of health conditions. Unfortunately, patients suffering from intense pain often give up or cut down on taking analgesics because of side effects of such treatment. New generation drugs create an opportunity for supporting therapies effectively. Persistent constipation is one of the more acute side effects of administration of opioids. This article gives an extensive coverage of the PAMORA group of drugs that eliminate opioid-induced constipation. It also proposes future directions for contemporary studies on the subject and other feasible therapeutic applications for this group of medicines. The molecule developed under the POIG.01.04.00-14-213/12 project “New intestinal drug for control of side effects of opioid therapies” is one of the prospects. [The project] researched structures based on opioid peptide analogs behaving like opioid antagonists vs. intestinal μ receptors. In addition, [the structures] feature properties that can be used in oncological therapies because they inhibit growth of malignant cells. The structures are being tested on animal models.
    Źródło:
    World Scientific News; 2018, 104; 400-410
    2392-2192
    Pojawia się w:
    World Scientific News
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Who stole the sugar? Recurrent hypoglycemia in three women
    Autorzy:
    Gontarz, Katarzyna
    Barczykowska, Anna
    Głowacka, Paulina
    Mędza, Aleksandra
    Młynarkiewicz, Małgorzata
    Obrębski, Jakub
    Szczurek, Małgorzata
    Wielewicka, Aleksandra
    Obołończyk, Łukasz
    Wiśniewski, Piotr
    Powiązania:
    https://bibliotekanauki.pl/articles/895783.pdf
    Data publikacji:
    2018-09-28
    Wydawca:
    Gdański Uniwersytet Medyczny
    Tematy:
    factitious disorder
    Munchausen syndrome
    hyperinsulinemia
    C-peptide
    Opis:
    This article presents 3 cases that highlight one of the factitious disorders named Munchausen Syndrome (MS). It is defined as intentional simulation or self-induction of disease symptoms to gain attention of others and to be perceived as an ill person. Early recognition of factitious disorders is a challenge for non-psychiatrists, as its clinical symptoms vary significantly among patients. In this paper we present three women with recurrent episodes of hypoglycemia, who were eventually diagnosed with MS. Our aims were to share the diagnostic clues that can suggest the presence of a factitious disorder, to highlight the analysis of patient’s medical history and to suggest the potential the ethical dilemmas involved in caring for such patients.
    Źródło:
    European Journal of Translational and Clinical Medicine; 2018, 1, 1; 70-75
    2657-3148
    2657-3156
    Pojawia się w:
    European Journal of Translational and Clinical Medicine
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Novel peptide recognized by RhoA GTPase
    Autorzy:
    Drulis-Fajdasz, Dominika
    Jelen, Filip
    Oleksy, Arkadiusz
    Otlewski, Jacek
    Powiązania:
    https://bibliotekanauki.pl/articles/1041207.pdf
    Data publikacji:
    2006
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    GrafGAP
    RhoA GTPase
    epitope mapping
    PDZRhoGEF
    peptide phage display
    Opis:
    A phage-displayed random 7-mer disulfide bridge-constrained peptide library was used to map the surface of the RhoA GTPase and to find peptides able to recognize RhoA switch regions. Several peptide sequences were selected after four rounds of enrichment, giving a high signal in ELISA against RhoA-GDP. A detailed analysis of one such selected peptide, called R2 (CWSFPGYAC), is reported. The RhoA - R2 interaction was investigated using fluorescence spectroscopy, chemical denaturation, and determination of the kinetics of nucleotide exchange and GTP hydrolysis in the presence of RhoA regulatory proteins. All measurements indicate that the affinity of the R2 peptide for RhoA is in the micromolar range and that R2 behaves as an inhibitor of: i) GDP binding to the apo form of RhoA (Mg2+- and nucleotide-free form of the GTPase), ii) nucleotide exchange stimulated by GEF (DH/PH tandem from PDZRhoGEF), and iii) GTP hydrolysis stimulated by the BH domain of GrafGAP protein.
    Źródło:
    Acta Biochimica Polonica; 2006, 53, 3; 515-524
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Discrete dynamic system oriented on the formation of prebiotic dipeptides from Rodes experiment
    Autorzy:
    Polanco, Carlos
    Samaniego, José
    Buhse, Thomas
    Castañón González, Jorge
    Powiązania:
    https://bibliotekanauki.pl/articles/1039201.pdf
    Data publikacji:
    2014
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    origins of life
    biogenesis
    dipeptides
    salt-induced peptide formation
    Opis:
    This work attempts to rationalize the possible prebiotic profile of the first dipeptides of about 4 billion years ago based on a computational discrete dynamic system that uses the final yields of the dipeptides obtained in Rode's experiments of salt-induced peptide formation (Rode et al., 1999, Peptides 20: 773-786). The system built a prebiotic scenario that allowed us to observe that (i) the primordial peptide generation was strongly affected by the abundances of the amino acid monomers, (ii) small variations in the concentration of the monomers have almost no effect on the final distribution pattern of the dipeptides and (iii) the most plausible chemical reaction of prebiotic peptide bond formation can be linked to Rode's hypothesis of a salt-induced scenario. The results of our computational simulations were related to former simulations of the Miller, and Fox & Harada experiments on amino acid monomer and oligomer generation, respectively, offering additional information to our approach.
    Źródło:
    Acta Biochimica Polonica; 2014, 61, 4; 717-726
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Free energy of helix propagation in short polyalanine chains determined from peptide growth simulations of La3+-binding model peptides. Comparison with experimental data
    Autorzy:
    Maciejczyk, Maciej
    Hermans, Jan
    Bierzyński, Andrzej
    Powiązania:
    https://bibliotekanauki.pl/articles/1041277.pdf
    Data publikacji:
    2006
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    thermodynamics
    peptide
    helix-coil equilibrium
    thermodynamic integration
    molecular dynamics
    Opis:
    Molecular dynamics (MD) is, at present, a unique tool making it possible to study, at the atomic level, conformational transitions in peptides and proteins. Nevertheless, because MD calculations are always based on a more or less approximate physical model, using a set of approximate parameters, their reliability must be tested by comparison with experimental data. Unfortunately, it is very difficult to find a peptide system in which conformational transitions can be studied both experimentally and using MD simulations so that a direct comparison of the results obtained in both ways could be made. Such a system, containing a rigid α-helix nucleus stabilized by La3+ coordination to a 12-residue sequence taken from an EF-hand protein has recently been used to determine experimentally the helix propagation parameters in very short polyalanine segments (Goch et al. (2003) Biochemistry 42: 6840-6847). The same parameters were calculated here for the same peptide system using the peptide growth simulation method with, alternatively, charmm 22 and cedar potential energy functions. The calculated free energies of the helix-coil transition are about two times too large for cedar and even three times too large for charmm 22, as compared with the experimental values. We suggest that these discrepancies have their origin in the incorrect representation of unfolded peptide backbone in solution by the molecular mechanics force fields.
    Źródło:
    Acta Biochimica Polonica; 2006, 53, 1; 121-130
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Conformational analysis of fragment of human Pin1 ww domain: influence of charged amino-acid residues on β-hairpin structure
    Autorzy:
    Makowska, J.
    Uber, D.
    Żmudzińska, W.
    Chmurzyński, L.
    Powiązania:
    https://bibliotekanauki.pl/articles/1935818.pdf
    Data publikacji:
    2014
    Wydawca:
    Politechnika Gdańska
    Tematy:
    peptide conformation
    β-hairpin
    hPin1 protein
    NMR
    Opis:
    We examined the effect of like-charged residues on the conformation of an original nine amino-acid-residue fragment of the human Pin1 WW domain (hPin1) with the following sequence: Ac-Arg-Met-Ser-Arg-Ser-Ser-Gly-Arg-Val-NH 2 (U9). This was facilitated by CD and NMR spectroscopic measurements, and molecular dynamics calculations. Our ear lier studies suggested that the presence of like-charged residues at the end of a short polypeptide chain composed of nonpolar residues could induce a chain reversal. For the U9 peptide, canonical MD simulations with NMR -derived restraints demonstrated the presence of ensembles of structures with a tendency to form a β -chain reversal. Additionally, thermal stabilities of the peptide under study were measured using differential scanning calorimetry ( DSC ). The estimated well defined phase transition point showed that conformational equilibria in the U9 peptide were strongly dependent on temperature.
    Źródło:
    TASK Quarterly. Scientific Bulletin of Academic Computer Centre in Gdansk; 2014, 18, 4; 343--349
    1428-6394
    Pojawia się w:
    TASK Quarterly. Scientific Bulletin of Academic Computer Centre in Gdansk
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Solution structure of conformationally restricted vasopressin analogues.
    Autorzy:
    Trzepałka, Emilia
    Oleszczuk, Marta
    Maciejczyk, Maciej
    Lammek, Bernard
    Powiązania:
    https://bibliotekanauki.pl/articles/1043316.pdf
    Data publikacji:
    2004
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    vasopressin
    X-PLOR
    NMR spectroscopy
    EDMC
    peptide conformation
    Opis:
    In recent years, a massive effort has been directed towards designing potent and selective antagonists of neurohypophyseal hormones substituted at position 3. Modification of vasopressin at position 3 with 4,4'-biphenylalanine results in pharmacologically inactive analogues. Chemically, this substitution appears to vary only slightly from those previously made by us (1-Nal or 2-Nal), which afforded potent agonists of V2 receptors. In this situation, it seemed worthwhile to study the structure of the analogues with 4,4'-biphenylalanine (BPhe) at position 3 in aqueous solution using NMR spectroscopy and total conformational analysis. This contribution is part of extensive studies aimed at understanding spatial structures of 3-substituted [Arg8]vasopressin analogues of different pharmacological properties. NMR data were used to calculate 3D structures for all the analogues using two methods, EDMC with the ECEPP/3 force field, and molecular dynamic with the simulated annealing (SA) algorithm. The structures obtained by the first method show a better fit between the NMR spectral evidence and the calculation for all the peptides.
    Źródło:
    Acta Biochimica Polonica; 2004, 51, 1; 33-49
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Synthetic extracellular matrix as a substrate for regenerative medicine
    Autorzy:
    Stodolak-Zych, Ewa
    Menaszek, Elżbieta
    Twardy, Aleksandra
    Ścisłowska-Czarnecka, Anna
    Boguń, Maciej
    Kolesińska, Beata
    Powiązania:
    https://bibliotekanauki.pl/articles/285740.pdf
    Data publikacji:
    2019
    Wydawca:
    Akademia Górniczo-Hutnicza im. Stanisława Staszica w Krakowie. Polskie Towarzystwo Biominerałów
    Tematy:
    synthetic extracellular matrix
    biomimetic
    peptide
    polysaccharides
    laboratory model
    Opis:
    The work presents materials characteristics of fibrous polysaccharide substrates (calcium alginate, CA) modified with short peptides. Three types of synthesized peptides (hexapeptides) were composed of: cysteine (C) and tryptophan (W) named - (WWC)2or cysteine (C) and tyrosine (Y) named (YYC)2 or phenyloalanine (F) named 6F. The peptides size distribution (DLS method) showed that they agglomerated in an alcohol medium. These results were used to select a modification method of the fibrous substrates i.e. the peptides were deposited on the fibrous alginate substrate by the electrospraying technique. Using this method three kinds of polysaccharide- peptides systems were obtained i.e.: CA/(WWC)2, CA/(YYC)2CA/6F. As a reference material, the pure calcium alginate fibrous substrate was used. The results of modification with short peptides were evaluated via scanning electron microscopy (SEM): small aggregates were observed (40-100 nm) on the surface of fibers, and the fibers size remained the same after modification (11-12 μm). The size of aggregates depended on the kind of short peptide; the smaller (40 nm) aggregates were observed when the peptide had only aromatic chain (6F), the bigger (<100 nm) ones were observed when the peptide had heterocyclic rings in the chain (WWC and YYC). All materials were contacted with osteoblast-like cells (MG-63) to test biocompatibility (cells viability after 3 and 7 days) and the results proved showed higher viability in the polysaccharide-peptide system which increased with the time of observation. The durability of polysaccharide-peptide systems was tested using the enzymatic assay: collagenase confirmed the stability of materials. The progress of degradation rate was observed using infrared spectroscopy (FTIR-ATR) - the ratio on bands with C-O and C-OH increased after degradation under in vitro conditions.Results of the investigations on the fibrous substrates have confirmed that the system is a good model of an extracellular matrix (ECM) due to its chemical composition and microstructure which both have biomimetic characteristics. Thus, it may be used as a filling of bone defects supporting the regeneration of the damaged tissue. Additionally, it may also serve as the model research system of ECM.
    Źródło:
    Engineering of Biomaterials; 2019, 22, 152; 10-15
    1429-7248
    Pojawia się w:
    Engineering of Biomaterials
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    DIAGNOSTIC POTENTIAL OF SELECTED SALIVARY PROTEOMICS FOR AUTONOMIC NERVOUS SYSTEM ACTIVITY ASSESSMENT
    Autorzy:
    Dobrek, Lukasz
    Powiązania:
    https://bibliotekanauki.pl/articles/2137800.pdf
    Data publikacji:
    2020-05-14
    Wydawca:
    Fundacja Edukacji Medycznej, Promocji Zdrowia, Sztuki i Kultury Ars Medica
    Tematy:
    saliva
    vasoactive intestinal peptide
    neuropeptide Y
    chromogranin A
    α-amylase
    Opis:
    The clinical assessment of autonomic nervous system (ANS) functioning, enabling the diagnosis of autonomic neuropathy present in the course of many diseases, is currently based on performing simple cardiovascular reflexes (Ewing tests), analyzing heart rate variability (HRV) or heart rate turbulence (HRT), examining skin sweating or recording neurophysiological tests (e.g. microneurography). Laboratory assessment of ANS function is very scarce and practically only includes the plasma assessment of noradrenaline as a surrogate for the biochemical indicator of sympathetic activity. Recently, the possibility of evaluation of selected compounds present in saliva as laboratory markers of not only oral diseases but also systemic diseases has been raised. This work focuses on a brief description of the anatomy and physiology of the salivary glands and describes the formation of saliva, its composition and the use of this bodily fluid in laboratory diagnostics. In addition, the paper specifically discusses the possibility of determining selected compounds that are considered to reflect autonomic activity. A review of the literature indicates primarily four proteomics: two neuropeptides (vasoactive intestinal peptide (VIP) and neuropeptide Y (NPY) that are co-transmitters in autonomic fibers, chromogranin A, a synaptic vesicle protein and α-amylase, a hydrolytic enzyme pre-digesting carbohydrates in the oral cavity. These are currently the most widely investigated agents for their usefulness as laboratory markers of ANS activity.
    Źródło:
    Acta Neuropsychologica; 2020, 18(2); 285-303
    1730-7503
    2084-4298
    Pojawia się w:
    Acta Neuropsychologica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Biological characteristics of a new antibacterial peptide and its antibacterial mechanisms against Gram-negative bacteria
    Autorzy:
    Pei, Z.
    Ying, X.
    Tang, Y.
    Liu, L.
    Zhang, H.
    Liu, S.
    Zhang, D.
    Wang, K.
    Kong, L.
    Gao, Y.
    Ma, H.
    Powiązania:
    https://bibliotekanauki.pl/articles/2087656.pdf
    Data publikacji:
    2018
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    antimicrobial peptide
    biological characteristics
    antibacterial mechanism
    Gram-negative bacteria
    Źródło:
    Polish Journal of Veterinary Sciences; 2018, 21, 3; 533-542
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Sequence-specific Ni(II)-dependent peptide bond hydrolysis in a peptide containing threonine and histidine residues
    Autorzy:
    Krężel, Artur
    Mylonas, Marios
    Kopera, Edyta
    Bal, Wojciech
    Powiązania:
    https://bibliotekanauki.pl/articles/1041163.pdf
    Data publikacji:
    2006
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    activation parameters
    complex formation
    nickel(II)
    peptide bond hydrolysis
    Opis:
    Previously we demonstrated that Ni(II) complexes of Ac-Thr-Glu-Ser-His-His-Lys-NH2 hexapeptide, representing residues 120-125 of human histone H2A, and some of its analogs undergo E-S peptide bond hydrolysis. In this work we demonstrate a similar coordination and reactivity pattern in Ni(II) complexes of Ac-Thr-Glu-Thr-His-His-Lys-NH2, its threonine analogue, studied using potentiometry, electronic absorption spectroscopy and HPLC. For the first time we present the detailed temperature and pH dependence of such Ni(II)-dependent hydrolysis reactions. The temperature dependence of the rate of hydrolysis yielded activation energy Ea = 92.0 kJ mol-1 and activation entropy ΔS≠ = 208 J mol-1 K-1. The pH profile of the reaction rate coincided with the formation of the four-nitrogen square-planar Ni(II) complex of Ac-Thr-Glu-Thr-His-His-Lys-NH2. These results expand the range of protein sequences susceptible to Ni(II) dependent cleavage by those containing threonine residues and permit predictions of the course of this reaction at various temperatures and pH values.
    Źródło:
    Acta Biochimica Polonica; 2006, 53, 4; 721-727
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Mass spectrometric analysis of head-to-tail connected cyclic peptides.
    Autorzy:
    Macht, Marcus
    Pelzing, Matthias
    Palloch, Petra
    Sauerland, Volker
    Volz, Jürgen
    Powiązania:
    https://bibliotekanauki.pl/articles/1044055.pdf
    Data publikacji:
    2001
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    electrospray
    post source decay
    MALDI
    mass spectrometry
    peptide sequencing
    Opis:
    Tandem mass spectrometry is an extremly useful tool for high sensitive sequence identification of peptides. In the case of cyclic peptides fragmentation can easily be performed for sequence analysis. However, analysis is usually tedious due to the lack of a defined beginning and end of the sequence. Since cyclic peptides are a highly interesting class of compounds especially for the pharmaceutical industry, ways have to be found to identify their structures. In this work we demonstrate how software and dedicated analytical strategies can be used for detailed analysis of these substances.
    Źródło:
    Acta Biochimica Polonica; 2001, 48, 4; 1109-1112
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    The use of serine protease from Yarrowia lipolytica yeast in the production of biopeptides from denatured egg white proteins
    Autorzy:
    Pokora, Marta
    Zambrowicz, Aleksandra
    Zabłocka, Agnieszka
    Dąbrowska, Anna
    Szołtysik, Marek
    Babij, Konrad
    Eckert, Ewelina
    Trziszka, Tadeusz
    Chrzanowska, Józefa
    Powiązania:
    https://bibliotekanauki.pl/articles/1038641.pdf
    Data publikacji:
    2017
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    Yarrowia lipolytica
    serine protease
    bioactive peptide
    antioxidant
    ACE-inhibitor
    Opis:
    Deriving non-conventional enzymes from cheaper sources than those used for commercially available enzymes may result in the production of hydrolysates with beneficial features, while drastically reducing the cost of hydrolysis. This is especially significant for enzymatic hydrolysis as a method of protein waste utilization. We have previously described the ability of non-commercial serine protease from Yarrowia lipolytica yeast to produce/release bioactive peptides from egg white protein by-products (EP). The enzymatic hydrolysis of EP was carried out for 24 h using the serine protease at an enzyme: substrate ratio of 1:30 (w/w). The obtained hydrolysate was characterized by protein degradation of 38% and also exhibited an antioxidant and cytokine-inducing activity. The isolation procedure (ultrafiltration and RP-HPLC) of bioactive peptides from the EP hydrolysate provided peptide fractions with significant antioxidant and ACE inhibitory activities. Three homogeneous and three heterogeneous peptide fractions were identified using MALDI-TOF/MS and the Mascot Search Results database. The peptides, mainly derived from ovalbumin, were composed of 2-19 amino-acid residues. We have thus demonstrated a novel ability of serine protease from Y. lipolytica to release biopeptides from an EP by-product.
    Źródło:
    Acta Biochimica Polonica; 2017, 64, 2; 245-253
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Using capillary electrophoresis to study methylation effect on RNA-peptide interaction.
    Autorzy:
    Mucha, Piotr
    Szyk, Agnieszka
    Rekowski, Piotr
    Agris, Paul
    Powiązania:
    https://bibliotekanauki.pl/articles/1043465.pdf
    Data publikacji:
    2003
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    methylated nucleosides
    methylation
    arginine methylation
    RN-peptide interaction
    capillary electrophoresis
    Opis:
    Methylation of RNA and proteins is one of a broad spectrum of post-transcriptional/translational mechanisms of gene expression regulation. Its functional signification is only beginning to be understood. A sensitive capillary electrophoresis mobility shift assay (CEMSA) for qualitative study of the methylation effect on biomolecules interaction is presented. Two RNA-peptide systems were chosen for the study. The first one consists of a 17-nucleotide analogue (+27-+43) of the yeast tRNAPhe anticodon stem and loop domain (ASLPhe) containing three of the five naturally occurring modifications (2'-O-methylcytidine (Cm32), 2'-O-methylguanine (Gm34) and 5-methylcytidine (m5C40)) (ASLPhe-Cm32,Gm34,m5C40) and a 15-amino-acid peptide (named tF2 : Ser1-Ile-Ser-Pro-Trp5-Gly-Phe-Ser-Gly-Leu10-Leu- Arg-Trp-Ser-Tyr15) selected from a random phage display library (RPL). A peptide-concentration-dependent formation of an RNA-peptide complex was clearly observable by CEMSA. In the presence of the peptide the capillary electrophoresis (CE) peak for triply methylated ASLPhe shifted from 18.16 to 20.90 min. Formation of the complex was not observed when an unmethylated version of ASLPhe was used. The second system studied consisted of the (+18)-(+44) fragment of the trans-activation response element of human immunodeficiency virus type 1 (TAR RNA HIV-1) and a 9-amino-acid peptide of the trans-activator of transcription protein (Tat HIV-1) Tat(49-57)-NH2 (named Tat1 : Arg49-Lys-Lys-Arg52-Arg-Gln-Arg-Arg- Arg57-NH2). In the presence of Tat(49-57)-NH2 a significant shift of migration time of TAR from 18.66 min to 20.12 min was observed. Methylation of a residue Arg52→Arg(Me)2, crucial for TAR binding, strongly disrupted formation of the complex. Only at a high micromolar peptide concentration a poorly shaped, broad peak of the complex was observed. CE was found to be an efficient and sensitive method for the analysis of methylation effects on interaction of biomolecules.
    Źródło:
    Acta Biochimica Polonica; 2003, 50, 3; 857-864
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Non-racemic mixture model: a computational approach
    Autorzy:
    Polanco, Carlos
    Buhse, Thomas
    Powiązania:
    https://bibliotekanauki.pl/articles/1038677.pdf
    Data publikacji:
    2017
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    origin of homochirality
    prebiotic peptide formation
    chiral asymmetry
    amino acids
    Opis:
    The behavior of a slight chiral bias in favor of l-amino acids over d-amino acids was studied in an evolutionary mathematical model generating mixed chiral peptide hexamers. The simulations aimed to reproduce a very generalized prebiotic scenario involving a specified couple of amino acid enantiomers and a possible asymmetric amplification through autocatalytic peptide self-replication while forming small multimers of a defined length. Our simplified model allowed the observation of a small ascending but not conclusive tendency in the l-amino acid over the d-amino acid profile for the resulting mixed chiral hexamers in computer simulations of 100 peptide generations. This simulation was carried out by changing the chiral bias from 1% to 3%, in three stages of 15, 50 and 100 generations to observe any alteration that could mean a drastic change in behavior. So far, our simulations lead to the assumption that under the exposure of very slight non-racemic conditions, a significant bias between l- and d-amino acids, as present in our biosphere, was unlikely generated under prebiotic conditions if autocatalytic peptide self-replication was the main or the only driving force of chiral auto-amplification.
    Źródło:
    Acta Biochimica Polonica; 2017, 64, 1; 17-19
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Metody chemicznej ligacji w syntezie peptydów i białek. Część 2
    Chemical ligation methods in the synthesis of peptides and proteins. Part 2
    Autorzy:
    Jędrzejewska, K.
    Kropidłowska, M.
    Wieczerzak, E.
    Powiązania:
    https://bibliotekanauki.pl/articles/172242.pdf
    Data publikacji:
    2017
    Wydawca:
    Polskie Towarzystwo Chemiczne
    Tematy:
    natywna chemiczna ligacja
    synteza peptydów
    tioester
    native chemical ligation
    peptide synthesis
    thioester
    Opis:
    Proteins are synthesized only by living organisms. Today, we are able to receive them by recombinant protein expression in bacterial cells. This technique is very useful and gives satisfactory amount of desirable material but it precludes the possibility of introduction of some chemical modifications that are often obligatory. For this reason, chemical synthesis of longer peptide chains is still important and is the object of scientists attention. Over the last century, notion of peptide synthesis took a new meaning. Nowadays, we know a number of innovative methods and also automated devices which help us to make progress in this area. Nevertheless, the synthesis of longer, more complicated peptide chains and proteins still constitutes a problem. Native chemical ligation (NCL) has facilitated the synthesis of numerous complex peptide and protein targets. Expansion of ligation techniques has allowed the entry of peptides into the world of therapeutic drugs [1]. NCL reactions are carried out in aqueous solution and give good yields. Due to mild conditions, NCL overcomes racemic and solubility problems encountered in classical peptide synthesis using protected fragments. The challenge is to synthesize the C-terminal thioester-containing peptide necessary for the transesterification reaction, which is the first step of linking the peptide fragments [2]. In this review we discuss the evolution, advantages and potential applications of chemical ligation reactions. In the first part of this article we described the utility of native chemical ligation approach to non-cysteine containing peptides. This part details a number of important approaches to the synthesis of peptides bearing a C-terminal thioester. Contemporary applications of these techniques to the total chemical synthesis of proteins are also presented.
    Źródło:
    Wiadomości Chemiczne; 2017, 71, 9-10; 747-761
    0043-5104
    2300-0295
    Pojawia się w:
    Wiadomości Chemiczne
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Investigation of asparagine deamidation in a SOD1-based biosynthetic human insulin precursor by MALDI-TOF mass spectrometry
    Autorzy:
    Bierczyńska-Krzysik, Anna
    Łopaciuk, Małgorzata
    Pawlak-Morka, Renata
    Stadnik, Dorota
    Powiązania:
    https://bibliotekanauki.pl/articles/1039302.pdf
    Data publikacji:
    2014
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    asparagine deamidation
    insulin precursor
    superoxide dismutase
    peptide mass fingerprinting
    MALDI-TOF MS
    Opis:
    A biosynthetic human insulin precursor displayed enhanced susceptibility to deamidation at one particular site. The present study was undertaken to monitor progress of precursor deamidation at successive manufacturing stages. MALDI-TOF/TOF MS in combination with controlled endoproteinase Glu-C and endoproteinase Asp-N proteolysis was used for rapid and unambiguous determination of deamidated residue within the investigated structure. Close inspection of isotopic distribution patterns of peptides resulting from enzymatic digestion enabled determination of distinct precursor forms occurring during the production process. Asn, Asp, isoAsp and succinimide derivatives of the amino acid at position 26 were unambiguously identified. These modifications are related to the leader peptide of a precursor encompassing amino acid sequence corresponding to that of superoxide dismutase [Cu-Zn] (SOD1 1, EC=1.15.1.1). Monitoring of precursor deamidation process at successive manufacturing stages revealed that the protein folding stage was sufficient for a prominent replacement of asparagine by aspartic and isoaspartic acid and the deamidated human insulin precursor constituted the main manufactured product. Conversion proceeded through a succinimide intermediate. Significant deamidation is associated with the presence of SNG motif and confirms results achieved previously on model peptides. Our findings highlight an essential role of the specific amino acid sequence on accelerated rate of protein deamidation. To our knowledge, this is the first time that such a dramatic change in the relative abundance of Asp and isoAsp resulting from protein deamidation process is reported.
    Źródło:
    Acta Biochimica Polonica; 2014, 61, 2; 349-357
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Wpływ różnych reszt β-aminokwasowych na zdolności koordynacyjne peptydów wobec jonów metali przejściowych
    The influence of different β-amino acid residues on coordinating abilities of peptides toward transition metal ions
    Autorzy:
    Krzciuk-Gula, Joanna
    Ledwoń, Patrycja
    Staśkiewicz, Agnieszka
    Latajkа, Rafał
    Powiązania:
    https://bibliotekanauki.pl/articles/972289.pdf
    Data publikacji:
    2020
    Wydawca:
    Polskie Towarzystwo Chemiczne
    Tematy:
    β-peptyd
    koordynacja
    jon metalu przejściowego
    β-peptide
    coordination
    transition metal ion
    Opis:
    Peptidomimetics are different groups of compounds which are the modifications of natural occurring peptides - mostly in the sense of the structure. Due to these kind of changes, the new, modified systems are more stable and could act as a tool dedicated in specific biological activity. One of the most important and developed group of peptidomimetics are β-peptides. This review discusses the coordination ability of peptides containing β-amino acid residues incorporated into their sequence. Special attention is given to the importance of βAla residue and metal binding affinity of transition metal ions, especially Cu2+ ion. The coordination process occurs analogously to peptides composed of a-amino acids. The complexes are usually formed initially by coordination of N-terminal amine group and subsequently, with increasing pH value, by deprotonation of amide bonds. The main difference can be observed in the stability and geometry of complexes. Namely, the stability constants of β-peptides are usually slightly lower than in the case of natural analogues. Furthermore, the review presents data according to coordination ability of peptides containing β-amino acids, such as: βAsp, βHis, βCys and βLys. In spite of differences between standard peptides and their analogues, containing β-amino acid residues, there are no very important differences in the model of their coordination with the transition metal ions. However, the comparison of β-peptides and their natural counterparts reveals interesting features, which can be useful for more effective designing of new compounds possessing expected properties.
    Źródło:
    Wiadomości Chemiczne; 2020, 74, 3-4; 285-310
    0043-5104
    2300-0295
    Pojawia się w:
    Wiadomości Chemiczne
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Wettability of chitosan-modified and lipid/polypeptide-coated peek surfaces
    Autorzy:
    Przykaza, Kacper
    Woźniak, Klaudia
    Jurak, Małgorzata
    Wiącek, Agnieszka Ewa
    Powiązania:
    https://bibliotekanauki.pl/articles/1034248.pdf
    Data publikacji:
    2019
    Wydawca:
    Sieć Badawcza Łukasiewicz - Polskie Towarzystwo Chitynowe
    Tematy:
    cyclosporine drug delivery
    lipid-peptide films
    plasma-activated PEEK
    surface free energy
    Opis:
    In the present paper, cold plasma-activated and chitosan-coated polyetheretherketone (PEEK) was covered with thin films of 1,2-dipalmitoyl-sn-glycero-3-phosphocholine, cholesterol, cyclosporine A, and their mixtures using the Langmuir-Blodgett technique. The thermodynamic function, i.e., surface free energy, of those systems was determined based on the contact angle hysteresis (CAH) approach. This parameter seems to be essential in determination of cell adhesion to polymeric materials and molecular interactions with living tissues. The obtained results show that the wettability and surface free energy of PEEK can be changed depending on the composition of the coating
    Źródło:
    Progress on Chemistry and Application of Chitin and its Derivatives; 2019, 24; 172-182
    1896-5644
    Pojawia się w:
    Progress on Chemistry and Application of Chitin and its Derivatives
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Immunohistochemical study on the expression of biologically active substances in the endocrine pancreas of the European bison
    Autorzy:
    Mozel, S.
    Szymańczyk, S.
    Krzysiak, M.
    Puzio, I.
    Zacharko-Siembida, A.
    Arciszewski, M.B.
    Powiązania:
    https://bibliotekanauki.pl/articles/2087293.pdf
    Data publikacji:
    2020
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    glucagon-like peptide-1
    glucagon
    insulin
    somatostatin
    immunohistochemistry
    pancreas
    European bison
    Źródło:
    Polish Journal of Veterinary Sciences; 2020, 23, 3; 333-340
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Methods of peptide conformation studies.
    Autorzy:
    Biedrzyński, Andrzej
    Powiązania:
    https://bibliotekanauki.pl/articles/1044052.pdf
    Data publikacji:
    2001
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    fluorescence resonance energy transfer
    NMR
    circular dichroism
    peptide conformation
    hydrogen exchange
    Opis:
    In solution most of the peptides assume multiple flexible conformations. Determination of the dominant conformers and evaluation of their populations is the aim of peptide conformation studies, in which theoretical and experimental methods play complementary roles. Molecular dynamics or Monte Carlo methods are quite effective in searching the conformational space accessible to a peptide but they are not able to estimate, precisely enough, the populations of various conformations. Therefore, they must be supplemented by experimental data. In this paper, a short review of the experimental methods, most widely used in peptide conformational studies, is presented. Among them NMR plays the leading role. Valuable information is also obtained from hydrogen exchange, fluorescence resonance energy transfer, and circular dichroism measurements. The advantages and shortcomings of these methods are discussed.
    Źródło:
    Acta Biochimica Polonica; 2001, 48, 4; 1091-1099
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Use of selected metal ions for the separation of peptides isolated from thermally processed string beans
    Wykorzystanie wybranych jonow metali w procesie rozdzialu peptydow izolowanych z fasoli szparagowej poddanej obrobce termicznej
    Autorzy:
    Karas, M
    Baraniak, B.
    Powiązania:
    https://bibliotekanauki.pl/articles/15240.pdf
    Data publikacji:
    2010
    Wydawca:
    Uniwersytet Warmińsko-Mazurski w Olsztynie / Polskie Towarzystwo Magnezologiczne im. Prof. Juliana Aleksandrowicza
    Tematy:
    metal ion
    separation
    peptide
    isolation
    string bean
    thermal process
    affinity chromatography
    Opis:
    Recent years have witnessed growing interest in research on the structure and properties of proteins and peptides as physiologically active dietary components. The above has spurred a new interest in the isolation of animal, plant and microbiological peptides and investigation of their biological activity. The isolation and separation of protein and peptide mixture is not an easy procedure. Immobilised Metal Ion Affinity Chromatography (IMAC) is increasingly often used in this process. Affinity chromatography relies on the specific interactions between amino acids, their reactive groups in peptides and metal ions. The objective of this study was to determine whether copper and nickel ions can be used for the separation of peptides isolated from string beans than had been blanched and heated in a microwave oven. In this study, peptides extracted with 1% trichloroacetic acid (TCA) from string beans that had been blanched and heated in a microwave oven, were separated by chromatography on columns with copper and nickel ions immobilised through iminodiacetic acid (IDA). Peptide concentrations of the separated fractions were determined. Peptides found in string beans had similar affinity for metal ions in the Cu > Ni sequence, with selectivity in the Ni > Cu sequence. Microwave heating of string beans decreases the peptide content of extracts isolated with 1% TCA. The resulting changes are dependent on the duration of the process and the type of heating medium. Affinity chromatography with the use of metal ions immobilized to iminodiacetic acid (IDA)-Sephadex G-25 may be successfully used for the separation of peptides isolated from string beans.
    W ostatnich latach obserwuje się coraz większe zainteresowanie badaniem struktury oraz właściwości białek i peptydów jako fizjologicznie aktywnych składników diety. W związku z powyższym wzrosło zainteresowanie izolowaniem i badaniem biologicznie aktywnych peptydów pochodzenia zwierzęcego, roślinnego oraz mikrobiologicznego. W skomplikowanym procesie rozdziału i izolowania mieszanin białek i peptydów coraz szersze zastosowanie znalazła chromatografia powinowactwa na unieruchomionych jonach metali – IMAC (Immobilized Metal Ion Affinity Chromatography). Chromatografia powinowactwa wykorzystuje specyficzne oddziaływania między aminokwasami oraz ich reaktywnymi ugrupowaniami w peptydach a jonami metali. Celem pracy było zbadanie przydatności jonów miedzi i niklu w procesie rozdziału peptydów izolowanych z fasoli szparagowej poddanej blanszowaniu i ogrzewaniu mikrofalami. Fasolę szparagową poddano odpowiedniej obróbce cieplnej (blanszowanie i ogrzewanie w kuchence mikrofalowej), a z uzyskanego surowca ekstrahowano peptydy 1% kwasem trójchlorooctowym (TCA) Następnie przeprowadzono rozdział chromatograficzny na kolumnach z unieruchomionymi jonami niklu i miedzi poprzez kwas iminodioctowy (IDA). W otrzymanych frakcjach oznaczono zawartość peptydów. Peptydy obecne w fasoli szparagowej charakteryzowały się zbliżonym powinowactwem do jonów metali, co przebiegało w kolejności Cu > Ni, natomiast selektywność układała się w kolejności Ni > Cu. Ogrzewanie fasoli szparagowej obniża poziom peptydów w ekstraktach izolowanych 1% TCA. Zmiany uzależnione są od czasu trwania procesu i rodzaju zastosowanego czynnika grzewczego. Metoda chromatografii powinowactwa z wykorzystaniem unieruchomionych jonów metali na schelatowanym kwasem iminodioctowym (IDA) żelu Sephadex G-25 może być z powodzeniem stosowana do rozdziału peptydów izolowanych z fasoli szparagowej.
    Źródło:
    Journal of Elementology; 2010, 15, 2; 291-300
    1644-2296
    Pojawia się w:
    Journal of Elementology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Próby aktywowania grupy karboksylowej kwasu 3-amino-1Hpirazolo-5-karboksylowego metodą azydkową
    Attempts of activating carboxylic group of 3-amine-1-Hpyrazole-5-carboxylic acid using azide method
    Autorzy:
    Kusakiewicz-Dawid, A.
    Masiekiewicz, E.
    Powiązania:
    https://bibliotekanauki.pl/articles/143389.pdf
    Data publikacji:
    2014
    Wydawca:
    Stowarzyszenie Inżynierów i Techników Przemysłu Chemicznego. Zakład Wydawniczy CHEMPRESS-SITPChem
    Tematy:
    amino pirazol
    hybrydy peptydowe
    metoda azydkowa
    aminopyrazole
    peptide hybrids
    azide method
    Opis:
    Opracowano metodę łączenia 3-acetamido-5-karboksy-1H-pirazolu od C- końca z aminokwasem białkowym, bez konieczności ochrony bocznych grup funkcyjnych pierścienia pirazolowego z wykorzystaniem aktywacji azydkowej. W modelowej reakcji zsyntezowano ester metylowy N-(3-acetamido-5-karbonylo-1H-pirazolo)glicyny.
    The method was developed for linking 3-acetamido-5-carboxy-1H-pyrazole with protein amino acid from C-end without necessity to protect side functional groups of pyrazole ring by means of azide activation. In the model reaction N-(3-acetamido-5-carbonyl-1Hpyrazole) glycine methyl ester was synthesized.
    Źródło:
    Chemik; 2014, 68, 4; 296-303
    0009-2886
    Pojawia się w:
    Chemik
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Inflammation- and axotomy-induced changes in cocaine- and amphetamine-regulated transcript peptide-like immunoreactive (CART-LI) nervous structures in the porcine descending colon
    Autorzy:
    Burlinski, P.J.
    Powiązania:
    https://bibliotekanauki.pl/articles/32044.pdf
    Data publikacji:
    2012
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    inflammation
    axotomy
    cocaine
    amphetamine
    peptide
    enteric nervous system
    nerve structure
    pig
    colon
    Opis:
    This study reports on changes in CART-like immunoreactive (CART-LI) nerve structures in the porcine descending colon during chemically driven inflammation and after axotomy. The distribution pattern of CART-LI nerve structures was studied using doublelabeling immunofluorescence technique in the circular muscle layer, myenteric (MP), outer submucous (OSP) and inner submucous plexuses (ISP) and also in the mucosal layer of the porcine descending colon in physiological conditions as well as under pathological factors. In the control animals, CART-LI perikarya have been shown to constitute 5.11% ± 0.64, 4.03% ± 1.17 and 0.05% ± 0.04 in MP, OSP and ISP, respectively. Changes in CART-immunoreactivity depended on the pathological factor and the part of the enteric nervous system (ENS) studied. Numbers of CART-LI perikarya amounted to 2.77% ± 0.64, 2.60% ± 0.36 and 0.26% ± 0.19 during chemically-induced colitis and 3.04% ± 0.88, 2.46% ± 0.8 and 0.43% ± 0.09 after axotomy in MP, OSP and ISP, respectively. Both studied pathological processes also caused an increase in the number of CART-LI nerve fibers in the circular muscle as well as in the mucosal layer.
    Źródło:
    Polish Journal of Veterinary Sciences; 2012, 15, 3
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    The impact of treatment exposure on diabetes biomarkers among Jordanian breast cancer women: a connection through FBG, C-peptide and HOMA-IR
    Wpływ leczenia na biomarkery cukrzycy u kobiet z rakiem piersi w Jordanii: związek z glikemią na czczo, peptydem C i wskaźnikiem HOMA-IR
    Autorzy:
    Al-Zeidaneen, Safaa A.
    Ahmad, Mousa N.
    Al-Ebuos, Ali D.
    Powiązania:
    https://bibliotekanauki.pl/articles/1029513.pdf
    Data publikacji:
    2017
    Wydawca:
    Medical Communications
    Tematy:
    C-peptide
    breast cancer stage
    fasting blood glucose
    menopausal status
    treatment exposure
    Opis:
    Background: Breast cancer is the most frequently occurring and life-threatening malignant tumor in women. The evidence that associates diabetes’ biomarkers with breast cancer is highly controversial. Aims: To evaluate diabetes’ biomarkers in breast cancer patients according to type of treatment exposure, breast cancer severity and menopausal status. Material and methods: A total of 396 breast cancer patients aged between 25 and 65 years attending breast cancer clinics were evaluated. The experimental design permitted to include 134 newly-diagnosed breast cancer patients who were not exposed to any type of interventions and 262 recently diagnosed breast cancer patients (up to three months). Recently, group members were subdivided in two subgroups to control exposure to therapy specially chemotherapy. The patients were further divided according to breast cancer stages and postmenopausal status. Diabetes biomarkers consisted of fasting blood glucose (FBG), C-peptide and HOMA-IR. Results: The high FBG was more prevalent in advance (24.1%) than early (10.6%) stage breast cancer. Compared with premenopausal breast cancer patients, postmenopausal breast cancer patients had higher prevalence of abnormal FBG (21.0% vs. 11.1%). The differences were also significant in the mean of FBG (103.0 ± 1.5 vs. 89.0 ± 0.0 mg/dL). In postmenopausal breast cancer patients, FBG was higher in the recently diagnosed whom expose to treatments including chemotherapy (106.5 ± 1.7 mg/dL vs. 126.2 ± 1.2 mm Hg) compared to the newly-diagnosed group whom not yet expose to any kind of treatment interventions. Conclusion: Diabetes was prevalent among breast cancer patients and it was higher in postmenopausal and advanced stage breast cancer women. The burden of diabetes on treatment expose breast cancer women tend to be high and warrants closer attention by health care provider to improved outcomes after diagnosis and treatment exposure.
    Wstęp: Rak piersi to najczęstszy zagrażający życiu nowotwór złośliwy u kobiet. Doniesienia dotyczące związku cukrzycy z rakiem piersi są wysoce kontrowersyjne. Cele: Analiza biomarkerów cukrzycy u pacjentek z rakiem piersi w zależności od ekspozycji na leczenie, stopnia zaawansowania nowotworu i statusu menopauzalnego. Materiał i metody: Do badania włączono 396 pacjentek z rakiem piersi w wieku od 25 do 65 lat, które zgłosiły się do klinik specjalizujących się w leczeniu tej choroby. Plan badania dopuszczał włączenie 134 pacjentek z nowo rozpoznanym nowotworem, które nie otrzymały jeszcze leczenia w żadnej postaci, oraz 262 pacjentek z nowotworem rozpoznanym w ciągu ostatnich trzech miesięcy. Następnie uczestniczki podzielono na dwie podgrupy w celu kontroli ekspozycji na leczenie, szczególnie chemioterapię. Pacjentki podzielono także na podstawie stopnia zaawansowania nowotworu i statusu menopauzalnego. Oceniono następujące biomarkery cukrzycy: glikemię na czczo, peptyd C oraz wskaźnik HOMA-IR. Wyniki: Wysoki poziom glukozy na czczo obserwowano częściej u chorych z zaawansowanym rakiem piersi (24,1%) niż u kobiet z nowotworem we wczesnym stadium zaawansowania (10,6%). W porównaniu z pacjentkami przed menopauzą chore po menopauzie charakteryzowało częstsze występowanie nieprawidłowej glikemii na czczo (21,0% vs 11,1%). Różnice były także istotne dla średniej wartości glukozy na czczo (103,0 ± 1,5 vs 89,0 ± 0,0 mg/dl). W przypadku kobiet po menopauzie wartości te były wyższe u chorych z rakiem piersi, które otrzymały leczenie, w tym chemioterapię (106,5 ± 1,7 mg/dl vs 126,2 ± 1,2 mm Hg), w porównaniu z nowo zdiagnozowanymi pacjentkami, u których nie rozpoczęto jeszcze leczenia w jakiejkolwiek postaci. Wnioski: W grupie badanych chorych cukrzyca była częstym problemem. Chorobę tą częściej obserwowano u kobiet po menopauzie i z zaawansowanym nowotworem. Odnotowano też istotną zależność między cukrzycą a ekspozycją na leczenie raka piersi. Na korelację tę należy zwrócić szczególną uwagę po rozpoznaniu choroby i wdrożeniu leczenia.
    Źródło:
    Current Gynecologic Oncology; 2017, 15, 4; 231-238
    2451-0750
    Pojawia się w:
    Current Gynecologic Oncology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Stereochemistry of insect kinin tetrazole analogues and their diuretic activity in crickets.
    Autorzy:
    Nachman, Ronald
    Coast, Geoffrey
    Kaczmarek, Krzysztof
    Williams, Howard
    Zabrocki, Janusz
    Powiązania:
    https://bibliotekanauki.pl/articles/1043327.pdf
    Data publikacji:
    2004
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    constrained insect kinin analogue
    tetrazole
    diuresis
    cis-peptide bond
    β-turn mimetic
    Opis:
    Insect kinin analogues of the sequence Phe-Phe-ψ[CN4]-Ala-Trp-Gly-NH2 containing (L-Phe2,L-Ala3) and (L-Phe2,D-Ala3) stereochemical variants of the tetrazole moiety, a mimic of the type VI β-turn, demonstrate significant agonist and partial antagonist activity, respectively, in a cricket diuretic bioassay. A comparison of the solution conformations of these two stereochemical variants indicates a structural basis for their divergent bioactivities. The (D-Phe2,D-Ala3) stereochemical variant was synthesized and found to demonstrate significant agonist activity. The results further define stereochemical requirements for the diuretic activity of insect kinins in crickets and provide valuable information for the design of biostable analogues capable of disrupting digestive and diuretic processes in pest insects.
    Źródło:
    Acta Biochimica Polonica; 2004, 51, 1; 121-127
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    A novel cis-peptide bond motif inducing β-turn type VI. The synthesis of enkephalin analogues modified with 4-aminopyroglutamic acid.
    Autorzy:
    Kaczmarek, Krzysztof
    Kaleta, Maciej
    Chung, Nga
    Schiller, Peter
    Zabrocki, Janusz
    Powiązania:
    https://bibliotekanauki.pl/articles/1044069.pdf
    Data publikacji:
    2001
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    constrained enkephalin analogues
    4-aminopyroglutamic acid
    cis-peptide bond
    β-turn mimetic
    Opis:
    A new pathway leading to a mixture of four isomers of 4-aminopyroglutamic acid is described. Michael type addition of Z-ΔAla-OMe to enolates prepared from acylaminomalonates, followed by hydrolysis and decarboxylation give protected 4-aminopyroglutamic acid with the 4-aminopyroglutamic acid with the cis:trans ratio approximately 3:2. This mixture was incorporated into Leu-enkephalin (position 2-3). After separation of peptides it appeared that all analogues were essentially inactive in guinea pig ileum and mouse vas deferens bioassays.
    Źródło:
    Acta Biochimica Polonica; 2001, 48, 4; 1159-1163
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Antybiotyki peptydowe i ich kompleksy z jonami metali
    Peptide antibiotics and their complexes with metal ions
    Autorzy:
    Stokowa-Sołtys, K.
    Powiązania:
    https://bibliotekanauki.pl/articles/171960.pdf
    Data publikacji:
    2018
    Wydawca:
    Polskie Towarzystwo Chemiczne
    Tematy:
    antybiotyki peptydowe
    metaloantybiotyki
    kompleksy jonów metali
    peptide antibiotics
    metalloantibiotic
    metal ion complexes
    Opis:
    Metal ions are essential for numerous antibiotics. They play a crucial role in the mechanism of action and may be involved in specific interactions with cell membrane or target molecules, such as: proteins and nucleic acids. Due to the fact that complexes usually poses a higher positive charge than free ligands, they might interact more tightly with DNA and RNA molecules. However, complexes may also form during antimicrobial agents application, because a lot of them possess functional groups which can bind metal ions present in physiological fluids. Many recent studies support a hypothesis that drugs may alter the serum metal ions concentration. Moreover, it has been shown that numerous complexes with antibiotics can cause DNA degradation, e.g. bleomycin which form stable complexes with redox metal ions and split the nucleic acids chain via the free radicals mechanism. Therefore, it is widely used in cancer therapy.
    Źródło:
    Wiadomości Chemiczne; 2018, 72, 7-8; 497-522
    0043-5104
    2300-0295
    Pojawia się w:
    Wiadomości Chemiczne
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Role of calcitonin gene related peptide in the modulation of intestinal circulatory, metabolic and myoelectric activity during ischemia-reperfusion
    Autorzy:
    Pawlik, W.W.
    Obuchowicz, R.
    Biernat, J.
    Sendur, R.
    Jaworek, J.
    Powiązania:
    https://bibliotekanauki.pl/articles/70549.pdf
    Data publikacji:
    2000
    Wydawca:
    Polskie Towarzystwo Fizjologiczne
    Tematy:
    metabolic activity
    intestinal circulation
    peptide
    blood flow
    ischaemia
    myoelectrical activity
    calcitonin gene
    oxygen uptake
    Źródło:
    Journal of Physiology and Pharmacology; 2000, 51, 4,2
    0867-5910
    Pojawia się w:
    Journal of Physiology and Pharmacology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Identification of CD4plus T cell epitopes from Staphylococcus aureus secretome using immunoinformatic prediction and molecular docking
    Autorzy:
    Francis, D.
    Kumar, A.
    Chittalakkottu, S.
    Powiązania:
    https://bibliotekanauki.pl/articles/2096359.pdf
    Data publikacji:
    2021
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    CD4+
    T cell
    epitope prediction
    secretory proteins
    Staphylococcus aureus
    peptide vaccine
    molecular docking
    Opis:
    One major reason for the lack of clinical success of Staphylococcus aureus vaccine candidates is the inability of the antigens to develop a CD4+ T cell-mediated immune response. Hence, it is important to identify CD4+ T cell antigens from S. aureus. CD4+ T cells are activated following the presentation of epitopes derived from exogenous proteins on HLA class II molecules. Fifty-nine secretory proteins of S. aureus were analyzed computationally for the presence of HLA class II binding peptides. Fifteen-mer peptides were generated, and their binding to 26 HLA class II alleles was predicted. The structural feasibility of the peptides binding to HLA-II was studied using molecular docking. Of the 16,724 peptides generated, 6991 (41.8%) were predicted to bind to any one of the alleles with an IC50 value below 50 nM. Comparative sequence analysis revealed that only 545 of the strong binding peptides are non-self in the human system. Approximately 50% of the binding peptides were monoallele-specific. Moreover, approximately 95% of the predicted strong binding non-self peptides interacted with the binding groove of at least one HLA class II molecule with a glide score better than !10 kcal/mol. On the basis of the analysis of the strength of binding, non-self presentation in the human host, propensity to bind to a higher number of alleles, and energetically favorable interactions with HLA molecules, a set of 11 CD4+ T cell epitopes that can be used as vaccine candidates was identified.
    Źródło:
    BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2021, 102, 1; 43-54
    0860-7796
    Pojawia się w:
    BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Targeting BACE with small inhibitory nucleic acids - a future for Alzheimers disease therapy?
    Autorzy:
    Nawrot, Barbara
    Powiązania:
    https://bibliotekanauki.pl/articles/1043280.pdf
    Data publikacji:
    2004
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    β-amyloid peptide
    deoxyribozymes
    small interfering RNAs
    hammerhead ribozymes
    Alzheimer's disease
    β-secretase
    Opis:
    β-Secretase, a β-site amyloid precursor protein (APP) cleaving enzyme (BACE), participates in the secretion of β-amyloid peptides (Aβ), the major components of the toxic amyloid plaques found in the brains of patients with Alzheimer's disease (AD). According to the amyloid hypothesis, accumulation of Aβ is the primary influence driving AD pathogenesis. Lowering of Aβ secretion can be achieved by decreasing BACE activity rather than by down-regulation of the APP substrate protein. Therefore, β-secretase is a primary target for anti-amyloid therapeutic drug design. Several approaches have been undertaken to find an effective inhibitor of human β-secretase activity, mostly in the field of peptidomimetic, non-cleavable substrate analogues. This review describes strategies targeting BACE mRNA recognition and its down-regulation based on the antisense action of small inhibitory nucleic acids (siNAs). These include antisense oligonucleotides, catalytic nucleic acids - ribozymes and deoxyribozymes - as well as small interfering RNAs (siRNAs). While antisense oligonucleotides were first used to identify an aspartyl protease with β-secretase activity, all the strategies now demonstrate that siNAs are able to inhibit BACE gene expression in a sequence-specific manner, measured both at the level of its mRNA and at the level of protein. Moreover, knock-down of BACE reduces the intra- and extracellular population of Aβ40 and Aβ42 peptides. An anti-amyloid effect of siNAs is observed in a wide spectrum of cell lines as well as in primary cortical neurons. Thus targeting BACE with small inhibitory nucleic acids may be beneficial for the treatment of Alzheimer's disease and for future drug design.
    Źródło:
    Acta Biochimica Polonica; 2004, 51, 2; 431-444
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Prospective proecological insecticides among the naturally occurring peptides
    Autorzy:
    Sobotka, W.
    Konopinska, D.
    Powiązania:
    https://bibliotekanauki.pl/articles/836235.pdf
    Data publikacji:
    1994
    Wydawca:
    Polskie Towarzystwo Parazytologiczne
    Tematy:
    proecological insecticide
    public health
    alga
    peptide
    bacteria
    fungi
    biological control agent
    insecticide
    plant
    crop protection
    Źródło:
    Annals of Parasitology; 1994, 40, 3
    0043-5163
    Pojawia się w:
    Annals of Parasitology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Cyclic AMP deficiency stimulates a stress condition in tobacco BY-2 cells
    Autorzy:
    Sgobba, A.
    Sabetta, W.
    Blanco, E.
    Paradiso, A.
    Viggiano, L.
    De Pinto, M.
    Powiązania:
    https://bibliotekanauki.pl/articles/80076.pdf
    Data publikacji:
    2013
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    conference
    cyclic amphipathic peptide
    stress condition
    tobacco
    BY-2 cell
    cell cycle progression
    plant
    subunit
    Źródło:
    BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2013, 94, 2
    0860-7796
    Pojawia się w:
    BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Lunasin – a bioactive peptide from triticale (X Triticosecale Wittmack) seeds, inhibits proliferation of cancer HeLa and SK-OV-3 cells
    Autorzy:
    Galbas, M.E.
    Porzucek, F.
    Selwet, M.
    Nowak, A.
    Slomski, R.
    Powiązania:
    https://bibliotekanauki.pl/articles/80518.pdf
    Data publikacji:
    2017
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    triticale
    Triticosecale
    seed
    bioactive peptide
    lunasin
    immunoblotting
    proliferation
    neoplastic cell
    SDS-PAGE electrophoresis
    HeLa cell
    Źródło:
    BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2017, 98, 3
    0860-7796
    Pojawia się w:
    BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Free radicals in chemistry, biology and medicine: contribution of radiation chemistry
    Autorzy:
    Bobrowski, K.
    Powiązania:
    https://bibliotekanauki.pl/articles/147946.pdf
    Data publikacji:
    2005
    Wydawca:
    Instytut Chemii i Techniki Jądrowej
    Tematy:
    free radicals
    pulse radiolysis
    oxidative stress
    ionic liquids
    long range electron transfer
    beta-amyloid peptide
    Opis:
    The scope of this article is limited to the concept of free radical and its historical background and a brief introduction to time-resolved techniques (pulse radiolysis, laser flash photolysis), which allowed direct observation of free radicals on real time. The selected contributions of pulse radiolysis to better understanding the role of free radical reactions in chemistry, biology and medicine are presented and some selected future research needs and opportunities in radiation chemistry are briefly addressed.
    Źródło:
    Nukleonika; 2005, 50,suppl.3; 67-76
    0029-5922
    1508-5791
    Pojawia się w:
    Nukleonika
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Conformational properties of N-acetyl-N-methyl-α,β-dehydroalanine N-methylamide
    Autorzy:
    Macedowska, Agnieszka
    Siodłak, Dawid
    Rzeszotarska, Barbara
    Powiązania:
    https://bibliotekanauki.pl/articles/1041296.pdf
    Data publikacji:
    2006
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    ab initio/DFT calculations
    N-alkylpeptides
    cis-trans isomerisation
    α,β-dehydroamino acids
    peptide design
    Opis:
    The conformational properties of Ac-Δ(Me)Ala-NHMe (N-acetyl-N-methyl-α,β-dehydroalanine N'-methylamide), as the simplest model of N-methyl-α,β-dehydroamino acids, was examined with theoretical methods and in comparison with Ac-ΔAla-NHMe and Ac-ΔAla-NMe2. The N-terminal amide of the Δ(Me)Ala residue easily adopts the configuration cis and the torsion angles φ, ψ are highly flexible. The Δ(Me)Ala residue is a conformational flexibilizer as compared to the parent ΔAla, which is a conformational stiffener. This seems to be the reason why Δ(Me)Ala is found in small natural cyclic peptides, where it ensures the conformational flexibility necessary for biological action.
    Źródło:
    Acta Biochimica Polonica; 2006, 53, 1; 227-232
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Prospective proecological insecticides among the naturally occurring peptides
    Autorzy:
    Sobotka, W.
    Konopińska, D.
    Powiązania:
    https://bibliotekanauki.pl/articles/2151480.pdf
    Data publikacji:
    1994
    Wydawca:
    Polskie Towarzystwo Parazytologiczne
    Tematy:
    proecological insecticide
    public health
    alga
    peptide
    bacteria
    fungi
    biological control agent
    insecticide
    plant
    crop protection
    Źródło:
    Wiadomości Parazytologiczne; 1994, 40, 3; 314-316
    0043-5163
    Pojawia się w:
    Wiadomości Parazytologiczne
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Antioxidant Activity of Hybrid Sturgeon (Huso dauricus × Acipenser schrenckii) Protein Hydrolysate Prepared Using Bromelain, Its Fractions and Purified Peptides
    Autorzy:
    Noman, Anwar
    Wang, Yuxia
    Zhang, Chao
    Abed, Sherif M.
    Powiązania:
    https://bibliotekanauki.pl/articles/2019350.pdf
    Data publikacji:
    2022-03-01
    Wydawca:
    Instytut Rozrodu Zwierząt i Badań Żywności Polskiej Akademii Nauk w Olsztynie
    Tematy:
    hybrid sturgeon
    enzymatic hydrolysis
    hydrolysis optimization
    bromelain
    degree of hydrolysis
    radical scavenging activity
    peptide purification
    Opis:
    Protein hydrolysates could be a natural and safer source of antioxidant peptides. The purpose of this study was to optimize the hydrolysis of Huso dauricus × Acipenser schrenckii sturgeon proteins using bromelain and purify antioxidant peptides from hydrolysate. The degree of hydrolysis of 18.69% was obtained under the optimal conditions and hydrolysate had 94.76% solubility, 902 nm particle size and high antioxidant activity. The IC50 for DPPH• and ABTS•+ scavenging activity were 3.14 and 3.81 mg/mL, respectively. The fraction of hydrolysate with a molecular weight of <1 kDa exhibited the highest antiradical activity against DPPH• with IC50 of 2.10 mg/mL. In turn, the IC50 of the most active fraction after the Sephadex G-15 separation was 1.77 mg/mL. The reverse phase high performance liquid chromatography (RP-HPLC) was used to purify the peptides from this fraction. The peptide with histidine, leucine and glycine (MW of 0.2955 kDa) exhibited the highest antioxidant activity (IC50 of 1.33 mg/mL). The obtained fractions and peptides with antioxidant activity could be used as natural substitutes for synthetic antioxidants, especially in food and pharmaceuticals.
    Źródło:
    Polish Journal of Food and Nutrition Sciences; 2022, 72, 1; 79-89
    1230-0322
    2083-6007
    Pojawia się w:
    Polish Journal of Food and Nutrition Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Fengycin or plipastatin? A confusing question in Bacilli
    Autorzy:
    Hussein, W.
    Powiązania:
    https://bibliotekanauki.pl/articles/79895.pdf
    Data publikacji:
    2019
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    fengycin
    plipastatin
    L-tyrosine
    Bacillus subtilis
    Bacillus amyloliquefaciens
    Bacillus atrophaeus
    epimerization
    nonribosomal peptide system
    Źródło:
    BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2019, 100, 1
    0860-7796
    Pojawia się w:
    BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Probing of Cu2+ ions binding to A β (5−16) peptide using ITC measurements and MD simulations
    Autorzy:
    Makowska, J.
    Żmudzińska, W.
    Wyrzykowski, D.
    Brzozowski, K.
    Zblewska, H.
    Chmurzyński, L.
    Powiązania:
    https://bibliotekanauki.pl/articles/1938620.pdf
    Data publikacji:
    2016
    Wydawca:
    Politechnika Gdańska
    Tematy:
    A β (5 − 16) fragments
    metal-peptide binding
    isothermal titration calorimetry
    molecular dynamics simulations
    Opis:
    It is shown that probably three residues: His6, His14 and His16 in the original sequence A β (1−42) serve as metal-binding sites for Cu2+ions. On the other hand, there is a possibility that only one of them plays a crucial role in the formation of the{A β (1-42)-Cu2+} complex. The isothermal titration calorimetry (ITC) measurements supported by molecular dynamic simulation (MD) with the NMR-derived restrains were used to investigate the interactions of Cu2+ with A β(5-16), a fragment of the A β(1-42) protein, with the following sequence: Ac-Arg-His-Asp-Ser-Gly-Tyr-Glu-Val-His-His-Gln-Lys-NH2, termed HZ1. The conditional thermodynamic parameters suggest that the formation of the Cu2+-HZ1 complex is both an enthalpy and entropy driven process under the experimental conditions. The studies presented here (after comparison with our previous results) show that the affinity of peptides to copper metal ions depends on two factors: the primary structure (amino acid composition) and the shape of the peptide conformation adopted.
    Źródło:
    TASK Quarterly. Scientific Bulletin of Academic Computer Centre in Gdansk; 2016, 20, 4; 409-416
    1428-6394
    Pojawia się w:
    TASK Quarterly. Scientific Bulletin of Academic Computer Centre in Gdansk
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Polymorphic analysis of peptide binding domain of major histocompatibility complex class I in domestic ducks
    Autorzy:
    Yu, S.
    Wu, J.
    Bai, J.
    Ding, Y.
    Qiu, W.
    Zhang, L.
    Powiązania:
    https://bibliotekanauki.pl/articles/2087551.pdf
    Data publikacji:
    2019
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    domestic duck
    highly variably sites
    MHC class I
    peptide binding domain
    phylogenetic tree
    polymorphism
    Źródło:
    Polish Journal of Veterinary Sciences; 2019, 2; 415-422
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    1,3-OXAZOLIDIN-5-ONES DERIVED FROM PROLINE AS CHIRAL COMPONENTS IN THE SYNTHESIS OF PREDICTIVE ENANTIOSELECTIVE COUPLING REAGENTS
    Autorzy:
    Kasperowicz-Frankowska, Katarzyna
    Kolesińska, Beata
    Gzik, Anna
    Jastrząbek, Konrad
    Kaminski, Zbigniew J.
    Powiązania:
    https://bibliotekanauki.pl/articles/895643.pdf
    Data publikacji:
    2018-08-31
    Wydawca:
    Polskie Towarzystwo Farmaceutyczne
    Tematy:
    enantioselective condensation
    peptide synthesis
    chirality transfer on nitrogen
    chiral nitrogen atom
    triazine coupling reagent
    Opis:
    1,3-Oxazolidin-5-ones derived from both enantiomers of proline and trichloroacetaldehyde were prepared and applied as amine component in the synthesis of chiral predictive triazine based coupling reagents. The usefulness of these reagents in the condensations yielding enantiomerically enriched products from racemic substrates was presented.
    Źródło:
    Acta Poloniae Pharmaceutica - Drug Research; 2018, 75, 4; 921-927
    0001-6837
    2353-5288
    Pojawia się w:
    Acta Poloniae Pharmaceutica - Drug Research
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Utylizacja odpadu nasion ostropestu plamistego II. Biologicznie czynne peptydy z odpadu nasion ostropestu plamistego
    Autorzy:
    Baranowska, B
    Kurzepa, K.
    Marczak, E.
    Szczucinska, A.
    Lipkowski, A.W.
    Powiązania:
    https://bibliotekanauki.pl/articles/833421.pdf
    Data publikacji:
    2003
    Wydawca:
    Instytut Hodowli i Aklimatyzacji Roślin
    Tematy:
    rosliny oleiste
    nasiona
    peptydy
    hydroliza enzymatyczna
    ostropest plamisty
    oil plant
    seed
    peptide
    enzymatic hydrolysis
    milk thistle
    Źródło:
    Rośliny Oleiste - Oilseed Crops; 2003, 24, 2; 725-732
    1233-8273
    Pojawia się w:
    Rośliny Oleiste - Oilseed Crops
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Anti-pathogenic properties of plant peptide hormone phytosulfokines [PSKs] and its selected analogues
    Autorzy:
    Bahyrycz, A.
    Saniewska, A.
    Konopinska, D.
    Powiązania:
    https://bibliotekanauki.pl/articles/55358.pdf
    Data publikacji:
    2008
    Wydawca:
    Sieć Badawcza Łukasiewicz - Instytut Przemysłu Organicznego
    Tematy:
    antipathogenic property
    Botrytis tulipae
    antifungal activity
    Phoma narcissi
    phytosulphokine-alpha
    plant peptide hormone
    phytosulphokine-beta analogue
    Opis:

    Phytosulfokine-α (PSK-α) (H-Tyr(SO3H)-Ile-Tyr(SO3H)-Thr-Gln- OH) (I), a sulfated growth factor universally found in both monocotyledons and dicotyledons, strongly promotes proliferation of plant cells in culture. The C-terminal truncated analog named PSK-β (Tyr(SO3H)-Ile-Tyr(SO3H)-Thr) (II) showed a 10-fold lower activity than that of the parent pentapeptide. Because PSK-α promotes proliferation and differentiation during the plant growth we undertook the studies on the influence of PSK-α on plant defense mechanisms in that period. In present studies on PSK-α (I), PSK-β (II), and its analogues, we performed a search of another biological properties. The aim of our investigation was evaluation of PSK-α, PSK-β and their selected analogues in relation to growth and development of plant pathogens, such as Phoma narcissi and Botrytis tulipae. In these studies we elaborated the synthesis of PSK-α or PSK-β and their 22 analogues modified by natural and non-natural amino acid residues. Peptides were synthesized by the solid phase method according to the Fmoc procedure on a Wang-resin. Free peptides were released from the resin by 95% TFA in the presence of EDT. Biological effect of these peptides was evaluated by test on the growth and development of pathogens of P. narcissi and B. tulipae.

    Źródło:
    Pestycydy; 2008, 1-2; 109-117
    0208-8703
    Pojawia się w:
    Pestycydy
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Nonprotein nitrogen compounds and proteolytic activity of albumin and globulin fractions in faba bean seeds
    Autorzy:
    Darewicz, M
    Kostyra, H.
    Powiązania:
    https://bibliotekanauki.pl/articles/1371367.pdf
    Data publikacji:
    1992
    Wydawca:
    Instytut Rozrodu Zwierząt i Badań Żywności Polskiej Akademii Nauk w Olsztynie
    Tematy:
    nitrogen compound
    peptide
    nucleic acid
    globulin fraction
    faba bean
    amino acid
    proteolytic activity
    seed
    albumin fraction
    Źródło:
    Polish Journal of Food and Nutrition Sciences; 1992, 01, 2; 109-113
    1230-0322
    2083-6007
    Pojawia się w:
    Polish Journal of Food and Nutrition Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Ca2plus waves and ROS in long distance root-to-shoot signalling
    Autorzy:
    Choi, W.
    Toyota, M.
    Hilleary, R.
    Swanson, S.
    Gilroy, S.
    Powiązania:
    https://bibliotekanauki.pl/articles/80330.pdf
    Data publikacji:
    2013
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    conference
    reactive oxygen species
    peptide
    signalling system
    plant stress
    stress tolerance
    gene expression
    signalling network
    Źródło:
    BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2013, 94, 2
    0860-7796
    Pojawia się w:
    BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Conformational properties of N-acetyl-L-alanine N',N'-dimethylamide.
    Autorzy:
    Siodłak, Dawid
    Rzeszotarska, Barbara
    Broda, Małgorzata
    Powiązania:
    https://bibliotekanauki.pl/articles/1043329.pdf
    Data publikacji:
    2004
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    ab initio/DFT calculations
    N-alkylpeptides
    β-turns
    alanine derivative
    N',N'-dimethylamides
    peptide design
    Opis:
    Ab initio/DFT analysis of the conformational properties of free Ac-Ala-NMe2 (N-acetyl-L-alanine-N',N'-dimethylamide) in terms of the N-H···O, N-H···N, C-H···O hydrogen bonds and Cδ+ = Oδ- dipole attractions was performed. The Ala residue combined with the C-terminal tertiary amide prefers an extended conformation and that characteristic of the (i + 1)th position of the βVIb turn. These can be easily remodelled into a structure compatible with the (i + 1)th position of the βII/βVIa turn. The residue has also the potential to adopt the conformation accommodated at both central positions of the βIII/βIII' turn or the (i + 1)th position of the βI/β'I turn.
    Źródło:
    Acta Biochimica Polonica; 2004, 51, 1; 137-143
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Właściwości koordynacyjne wybranych cyklicznych hormonów peptydowych oraz ich pochodnych
    Coordination properties of selected cyclic peptide hormones and their analogues
    Autorzy:
    Witak, Weronika
    Marciniak, Aleksandra
    Powiązania:
    https://bibliotekanauki.pl/articles/1409943.pdf
    Data publikacji:
    2021
    Wydawca:
    Polskie Towarzystwo Chemiczne
    Tematy:
    hormony peptydowe
    peptydy
    wiązanie disulfidowe
    jony metali
    peptide hormones
    cyclic peptides
    disulfide bridge
    metal ions
    Opis:
    Hormones are a heterogeneous, significant compounds, responsible for proper functioning of living organisms, produced by specialized cells, tissues and glands. Theirs main role is signals transmission to target tissues, responsible for the right working of the whole organism. Dysfunctions of the hormones homeostasis balance lead to disease states [1-3]. Recently, scientists have paid attention to role of the metal ions in the proper synthesis of these compounds and functioning of human body [4]. More and more scientific works explain the complicated roles of metals as beneficial factors that stimulate the conformation of peptides. Metal ions are responsible for biological properties and influence of hormones binding to appropriate receptors, but also adverse factors [4,5]. The search for an answer to the question about the metal - hormone relationship has led to the development of a new, interdisciplinary studies: metalloendocrinology, linking inorganic chemistry with endocrinology [4]. In this work, we present the literature data that relate to metal - peptide hormone interactions. We focused on cyclic hormones with a disulfide bridge and their analogues. In our review we have focused on selected natural cyclic peptide hormones: oxytocin, vasopressin, somatostatin, hepcidin and amylin. The studies on the coordination abilities showed that transition metal ions, such as copper(II), zinc(II) or nickel(II), form stable complexes with described peptides. Metal ions actively participate in many phenomena. They have played role in the formation of amylin aggregates in patients with type 2 diabetes [6]. The high ability to copper(II) by hepcidin may have an effect on its homeostasis [7]. Stable complexes of oxytocin and vasopressin facilitate binding with appropriate receptors for these peptides [8].
    Źródło:
    Wiadomości Chemiczne; 2021, 75, 5-6; 799-821
    0043-5104
    2300-0295
    Pojawia się w:
    Wiadomości Chemiczne
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Guanylin and related peptides
    Autorzy:
    Beltowski, J.
    Powiązania:
    https://bibliotekanauki.pl/articles/69588.pdf
    Data publikacji:
    2001
    Wydawca:
    Polskie Towarzystwo Fizjologiczne
    Tematy:
    gastrointestinal tract
    lymphoguanylin
    uroguanylin
    peptide
    guanylin
    Escherichia coli
    cystic fibrosis
    protein kinase G
    enterotoxin
    diarrhea
    Źródło:
    Journal of Physiology and Pharmacology; 2001, 52, 3
    0867-5910
    Pojawia się w:
    Journal of Physiology and Pharmacology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Wyjaśnienie elementów homeostazy niklu(II) i cynku(II) u bakterii i grzybów
    Explaining elements of nickel(II) and zinc(II) homeostasis in bacteria and fungi
    Autorzy:
    Rowinska-Żyrek, M.
    Powiązania:
    https://bibliotekanauki.pl/articles/172088.pdf
    Data publikacji:
    2018
    Wydawca:
    Polskie Towarzystwo Chemiczne
    Tematy:
    transport Zn2+
    transport Ni2+
    homeostaza metali drobnoustrojach
    układ metal-peptyd
    struktura układów metal-peptyd
    termodynamika układów metal-peptyd
    Zn2+ transport
    Ni2+ transport
    microbial metal homeostasis
    metal-peptide structure
    metal-peptide thermodynamics
    Opis:
    In the last 30 years, no new class of antibiotic was developed, and resistance to these already existing has increased dramatically. It seems reasonable to search for new classes therapeutics, targeting metabolic pathways, which standard therapies do not aim at. One of the biggest obstacles in finding effective and specific antibacterial and antifungal agents, which do not cause serious side effects in patients, is due to the fact that micro-organisms share many basic metabolic pathways with their human hosts. One of the significant differences may be the transport system and homeostasis of Zn2+ and Ni2+. The review sheds new light on the homeostasis of the two metals in bacteria and fungi. The main points are: (i) determination of Zn2+ binding sites on the C. albicans Pra1 zincophore and in the N-terminal domain of the C. albicans Zrt1 zinc transporter; description of the geometry and thermodynamics of such binding (Fig. 5 and 6); (ii) understanding of the bioinorganic chemistry of zincophore based Zn2+ transport (understanding Pra1-Zrt1 interactions); suggesting how Zn2+ is delivered from the zincophore to the zinc transporter (Fig. 7); (iii) defining the specificity of zincophore-based transport; showing that they can also transport Ni2+ ions; (iv) pointing out Zn2+ binding sites on amylin1-19 and pramlintide – amylin’s non-aggregating analogue; describing the thermodynamics of the process (Fig. 10) and suggesting the potential effect of Zn2+ coordination on the antimicrobial effectiveness of amylin (Fig. 11 and 12); (v) defining how the non-coordinating poly- Gln region affect the structure and how it increases the thermodynamic stability of nickel complexes of the N-terminal region Hpn-like, a microbial Ni2+ storage protein (Fig. 13); (vi) indicating the specific regions of proteins with polyHis and polyGln regions, which are most likely to bind Ni2+ and Zn2+; (vii) explaining the effect of pH and Ni2+ binding to the N-terminal domain of HypA, a bacterial protein involved in the maturation of hydrogenase (Fig. 14 and 15); (viii) explaining the average efficiency and selectivity of HupE, a bacterial Ni2+ transporter (Fig. 16 and 17). This new piece of knowledge is an interesting contribution to the beautiful, basic bioinorganic chemistry, which allows for a better understanding of basic mechanisms in biology and can be the basis in the design of effective, specific and selective drugs to be used in anti-microbial therapy, e.g. of traditional drugs combined with a part of a zincophore, which is specifically recognized by the fungus. First biological studies, which show that Candida albicans recognizes the C-terminal region Pra1, have already been carried out (see Figure 8 and its description).
    Źródło:
    Wiadomości Chemiczne; 2018, 72, 7-8; 469-496
    0043-5104
    2300-0295
    Pojawia się w:
    Wiadomości Chemiczne
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Effect of trypsin modulating oostatic factor (TMOF) on trypsin and chymotrypsin in Glyphodes pyloalis Walker (Lep.: Pyralidae) and Hyphantria cunea Drury (Lep.: Arctiidae)
    Autorzy:
    Ajamhassani, M.
    Jalali Sendi, J.
    Ghadamyary, M.
    Borovsky, D.
    Powiązania:
    https://bibliotekanauki.pl/articles/55071.pdf
    Data publikacji:
    2011
    Wydawca:
    Sieć Badawcza Łukasiewicz - Instytut Przemysłu Organicznego
    Tematy:
    trypsin
    modulating oostatic factor
    chymotrypsin
    Glyphodes pyloalis
    Lepidoptera
    Pyralidae
    Hyphantria cunea
    Arctiidae
    peptide hormone
    larva
    biosynthesis
    Opis:
    In the present study, the effect of three concentrations of TMOF (7.5, 15 and 30 μg dissolved in water) on trypsin and chymotrypsin biosynthesis in 4th instar larvae of Glyphodes pyloalis walker and Hyphantria cunea Drury were studied 24 h and 48 h after injecting. Our results indicated that in G. pyloalis, lower concentrations (7.5 and 15 μg) inhibited trypsin biosynthesis at 24 h after injection. (P < 0.0001). TMOF, however, did not significantly affect trypsin biosynthesis at 48 h. In H. cunea, at 48 h after the injection, all concentrations (7.5, 15 and 30 μg per larvae) significantly inhibited trypsin biosynthesis (P < 0.05). Injections of TMOF did not significantly affect chymotrypsin in both insects. Although, in G. pyloalis, chymotrypsin activity decreased about 25% at 48 h after injection.
    Źródło:
    Pestycydy; 2011, 1-4
    0208-8703
    Pojawia się w:
    Pestycydy
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Actions of several substituted short analogues of porcine galanin on isolated rat fundus strips: a structure-activity relationship
    Autorzy:
    Korolkiewicz, R.P.
    Konstanski, Z.
    Rekowski, P.
    Ruczynski, J.
    Szyk, A.
    Grzybowska, M.
    Korolkiewicz, K.Z.
    Petrusewicz, J.
    Powiązania:
    https://bibliotekanauki.pl/articles/71045.pdf
    Data publikacji:
    2001
    Wydawca:
    Polskie Towarzystwo Fizjologiczne
    Tematy:
    gastric fundus
    genitourinary tract
    peptide
    gut
    peripheral nervous system
    endocrine system
    amino acid
    smooth muscle
    rat
    Źródło:
    Journal of Physiology and Pharmacology; 2001, 52, 1
    0867-5910
    Pojawia się w:
    Journal of Physiology and Pharmacology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Modele eksperymentalne w badaniach zewnątrzkomórkowego transportu miedzi
    Experimental models in studies of extracellular copper transport
    Autorzy:
    Bal, Wojciech
    Powiązania:
    https://bibliotekanauki.pl/articles/2200581.pdf
    Data publikacji:
    2022
    Wydawca:
    Polskie Towarzystwo Chemiczne
    Tematy:
    copper(II)
    peptide
    complexation
    reaction kinetics
    biological mechanisms
    miedź(II)
    peptyd
    kompleksowanie
    kinetyka reakcji
    mechanizmy biologiczne
    Opis:
    The biological relevance of proteins and peptides for Cu(II) biology, including the extracellular transport of this element, is commonly estimated by studying stabilities and structures of their complexes. However, our experimental studies on the kinetics of formation of Cu(II) complexes of ATCUN/NTS and Xaa-His-R peptides, considered to be key actors in extracellular copper biology, revealed novel long-lived reaction intermediates. These intermediates, rather than the final reaction products fulfil the chemical criteria for actual biocomplexes derived from biological studies. Our research clearly demonstrated that understanding of the kinetic aspect of interactions is indispensable for realistic modeling of biological interactions of metal ions.
    Źródło:
    Wiadomości Chemiczne; 2022, 76, 5-6; 456-466
    0043-5104
    2300-0295
    Pojawia się w:
    Wiadomości Chemiczne
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Metody chemicznej ligacji w syntezie peptydów i białek. Część 1
    Chemical ligation methods in the synthesis of peptides and proteins. Part 1
    Autorzy:
    Kropidłowska, M.
    Jędrzejewska, K.
    Wieczerzak, E.
    Powiązania:
    https://bibliotekanauki.pl/articles/171570.pdf
    Data publikacji:
    2017
    Wydawca:
    Polskie Towarzystwo Chemiczne
    Tematy:
    natywna chemiczna ligacja
    NCL
    kinetycznie kontrolowana ligacja
    KCL
    synteza peptydów
    native chemical ligation
    kinetically controlled ligation
    peptide synthesis
    Opis:
    Proteins are biological macromolecules affecting very important functions in the body. They are involved in many biochemical processes. They can perform catalytic functions acting as enzymes. They also participate in the transport of many small molecules and ions – for example one molecule of hemoglobin carries four molecules of oxygen. In addition, proteins serve as antibodies and are involved in transmission of nerve impulses as receptor proteins. Because peptides and proteins perform so important functions, to study them it is essential to obtain these compounds in the greatest possible amounts. The compounds can be obtained generally by three main methods: • by isolation of the native peptides and proteins • by expression in microorganisms • by chemical synthesis. Each of the above methods has its advantages and disadvantages, but only the chemical synthesis gives the possibility to introduce modifications to the structure of the resulting protein, such as the insertion of new functional groups, to give the product in the final form and with satisfactory yield. In this review we present the application of chemical ligation methods in the synthesis of peptides and proteins. We describe in details mechanism of native chemical ligation method and the conditions necessary to carry the reaction [1]. The synthesis of long polypeptide chains by kinetically controlled ligation (KCL) is also depicted [2]. This part of the paper also details a number of approaches to noncysteine containing peptides by chemical ligation methods.
    Źródło:
    Wiadomości Chemiczne; 2017, 71, 9-10; 727-746
    0043-5104
    2300-0295
    Pojawia się w:
    Wiadomości Chemiczne
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Identyfikacja białek z wykorzystaniem techniki Peptide Mass. Część I - charakterystyka eksperymentu identyfikacji
    The identification of proteins by Peptide Mass Fingerprinting (PMF). Part I - properties of the identification experiment
    Autorzy:
    Kamińska, H.
    Podbielska, H.
    Powiązania:
    https://bibliotekanauki.pl/articles/261316.pdf
    Data publikacji:
    2011
    Wydawca:
    Politechnika Wrocławska. Wydział Podstawowych Problemów Techniki. Katedra Inżynierii Biomedycznej
    Tematy:
    proteomika
    identyfikacja protein
    spektrometria masowa
    schematy scoringu
    proteomics
    identification of proteins
    mass spectrometry
    peptide mass fingerprinting
    scoring schemes
    Opis:
    Wprowadzenie w spektrometrach jonizacji typu MALDI zrewolucjonizowało proces identyfikacji białek. Automatyzacja procesu identyfikacji oraz bezpośrednie połączenie analizy spektrometrem masowym z separacją białek dwuwymiarową elektroforezą żelową (2D-GE) pociągnęły za sobą znaczny rozwój proteomiki. Późniejszy rozrost proteomicznych baz danych pozwolił na zwiększenie dokładności identyfikacji, z wykorzystaniem pierwszej w historii techniki wydajnej identyfikacji białek – peptide mass fingerprinting, w skrócie: PMF. Metoda peptide mass fingerprinting pozwala identyfikować białka z widm masowych uzyskanych w wyniku analizy próbki spektrometrem masowym. Przez wzgląd na powszechność stosowania metody, jak i ciągle obserwowane jej ulepszenia, autorzy postanowili podsumować obecny stan wiedzy w tym zakresie. Praca została podzielona na dwie części: w pierwszej znajduje się opis historii powstania metody PMF wraz z charakterystyką części eksperymentalnej i opisem najpopularniejszych baz danych stosowanych przy identyfikacji, natomiast druga część pracy jest poświęcona zagadnieniom algorytmicznym, związanym z wyszukiwaniem w bazie danych protein najlepiej odzwierciedlających białko analizowane w próbce. Specyfikacja eksperymentu w pierwszej części pracy uwzględnia zarówno opis metody separacji, trawienia białek w próbce, jak i późniejszej ich analizy z wykorzystaniem spektrometru masowego. Eksperymentalne fazy metody PMF są opisane z uwzględnieniem ich cech biochemicznych, mających wpływ na dalsze etapy schematu identyfikacji.
    The development of MALDI ionization method in mass spectrometers, had revolutionized the protein identification procedure. The automation of an identification procedure and the mass spectrometry direct connection to the protein separation with the two-dimensional gel electrophoresis (2D-GE) implicated the significant proteomics development. The later growth of the proteomics databases contributed to the enhancement of the identification accuracy, by using the first method of effective protein identification in the history: the peptide mass fingerprinting (PMF). The peptide mass fingerprinting enabled the protein identification from the mass spectra acquired by the mass spectrometry sample analysis. Due to the common use of method and its continuous improvements, the authors decided to summarize the current state of the knowledge in this field of science. The publication is divided into two parts. The first one is devoted to the origins of PMF scheme, the characteristics of its experimental part and a description of the most popular databases used in the identification procedure. The second part relates to the algorithmic issues of searching the database protein, which reflects the sample content in the best way. The experiment specification in the first part takes into the consideration the description of separation and sample digestion methods, as well as the later protein sample analysis by the mass spectrometer. The experimental steps of the PMF method are described according to their biochemical properties, having an impact for the later stages of the identification procedure.
    Źródło:
    Acta Bio-Optica et Informatica Medica. Inżynieria Biomedyczna; 2011, 17, 2; 153-160
    1234-5563
    Pojawia się w:
    Acta Bio-Optica et Informatica Medica. Inżynieria Biomedyczna
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Secretin, cholecystokinin and the biliary canalicular secretion in the rat
    Autorzy:
    Romanski, K W
    Bochenek, W.J.
    Unold, J.
    Szczepaniak, M.
    Powiązania:
    https://bibliotekanauki.pl/articles/70531.pdf
    Data publikacji:
    1995
    Wydawca:
    Polskie Towarzystwo Fizjologiczne
    Tematy:
    biliary tree
    secretin
    biliary canalicular secretion
    gastrointestinal hormone
    gastrointestinal peptide
    rat
    biliary canalicular flow
    cholecystokinin
    bile acid
    bile
    Źródło:
    Journal of Physiology and Pharmacology; 1995, 46, 2
    0867-5910
    Pojawia się w:
    Journal of Physiology and Pharmacology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Effects of arginine substitutions on the cardioinhibitory activity of the Led-NPF-I neuropeptide
    Autorzy:
    Marciniak, P.
    Slocinska, M.
    Bednarz, P.
    Grodecki, S.
    Konopinska, D.
    Rosinski, G.
    Powiązania:
    https://bibliotekanauki.pl/articles/55015.pdf
    Data publikacji:
    2009
    Wydawca:
    Sieć Badawcza Łukasiewicz - Instytut Przemysłu Organicznego
    Tematy:
    cardioinhibitory activity
    arginine substitution
    mealworm beetle
    Zophobas atratus
    giant mealworm beetle
    Led-NPF-I-peptide
    insect
    beetle
    neuropeptide
    Opis:
    Effects of structural changes on the cardioinhibitory activity of the Led-NPF-I peptide (Ala-Arg-Gly-Pro-Gln-Leu-Arg-Leu-Arg-Phe-amide) were examined by replacing Arg residues in positions 2, 7 and 9. Replacement of L-Arg2 with another basic amino acid, such as Lys, His or D-Arg, did not abolish but rather promoted cardioinhibitory activity in giant mealworm beetle Zophobas atratus Fab. Agonistic peptides were also obtained by substitution of Arg residue in position 7 with Lys or D-Arg, and Arg in position 9 with His or D-Arg, respectively. All these analogues showed stronger cardioinhibitory effects than the native peptide at low concentration (10-9 M), and [Lys7]-, [D-Arg7]- and [D-Arg9]-Led-NPF-I also at the higher concentration (10-6 M). However, substitutions of the Arg residues in position 7 with His or in position 9 with Lys caused a loss of the cardioinhibitory activity. In addition, the replacement of Arg residues in all three positions with Lys or Orn caused a reduction of cardioinhibitory activity, although a single substitution of Arg in positions 2 or 7 with Lys yielded agonistic peptides. We conclude that the Arg2 position in the N-terminal region is more tolerant to structural modification than the other two Arg positions located in the C-terminal region.
    Źródło:
    Pestycydy; 2009, 1-4; 71-78
    0208-8703
    Pojawia się w:
    Pestycydy
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Beef as a source of bioactive components
    Mięso wołowe jako źródło składników bioaktywnych
    Autorzy:
    Sadowska, A.
    Waszkiewicz-Robak, B.
    Nowosinska, K.
    Batogowska, J.
    Rakowska, R.
    Powiązania:
    https://bibliotekanauki.pl/articles/796400.pdf
    Data publikacji:
    2014
    Wydawca:
    Szkoła Główna Gospodarstwa Wiejskiego w Warszawie. Wydawnictwo Szkoły Głównej Gospodarstwa Wiejskiego w Warszawie
    Tematy:
    beef
    bioactive component
    source
    protein
    amino acid
    peptide
    collagen
    nutritional value
    B-group vitamin
    iron
    zinc
    copper
    Opis:
    Beef is classified as the most valuable meat in terms of nutritional value. It contains a number of valuable bioactive substances, preferably affecting the functioning of the body. It is a rich source of protein, amino acids, bioactive peptides (including taurine, carnosine, creatine, carnitine) and contains collagen rich with hydroxy acids (hydroxyproline and hudroksylizyne). Beef is also characterized with a high content of B vitamins (especially B₁₂ vitamin), minerals including most of all easily digestible iron, zinc and copper. Moreover, from a nutritional point of view beef fat contains lots of valuable components, such as conjugated linoleic acid (CLA), significant amounts of fatty acids from n-3 group and coenzyme Q₁₀. In comparison with meat obtained from other animals beef derived from animal meat breed is characterized by a low content of intramuscular fat (2–3%) which is connected with low content of saturated fatty acids. At the same time according to the latest data consuming too much red meat can pose a risk to health due to the development of many diseases.
    Mięso wołowe pod względem odżywczym zaliczane jest do najwartościowszych mięs. Mięso to jest źródłem wielu cennych substancji bioaktywnych, korzystnie oddziałujących na funkcjonowanie organizmu, bogatym źródłem pełnowartościowego białka, bioaktywnych aminokwasów i peptydów (m.in. tauryny, karnozyny, kreatyny, karnityny) oraz zawiera bogate w hydroksykwasy (hydroksyprolinę i hydroksylizynę) białka kolagenowe. Mięso wołowe charakteryzuje się także wysoką zawartością witamin z grupy B (głównie witaminy B₁₂), składników mineralnych, w tym przede wszystkim łatwo przyswajalnego żelaza, cynku i miedzi. Ponadto tłuszcz wołowy zawiera wiele cennych, z żywieniowego punktu widzenia, komponentów, takich jak: sprzężony kwas linolowy (CLA), znaczne ilości kwasów tłuszczowych z rodziny n-3, czy koenzym Q₁₀. Mięso wołowe pochodzące od zwierząt ras mięsnych cechuje się niską zawartością tłuszczu śródmięśniowego (ok. 2–3%) i z tym związaną niską zawartością kwasów tłuszczowych nasyconych w porównaniu do mięsa pochodzącego od innych gatunków zwierząt. Jednocześnie według najnowszych danych spożywanie zbyt dużych ilości mięsa czerwonego może stwarzać zagrożenie dla zdrowia wskutek rozwoju wielu chorób.
    Źródło:
    Zeszyty Problemowe Postępów Nauk Rolniczych; 2014, 576
    0084-5477
    Pojawia się w:
    Zeszyty Problemowe Postępów Nauk Rolniczych
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Dansylated analogues of the opioid peptide [Dmt1]DALDA: in vitro activity profiles and fluorescence parameters.
    Autorzy:
    Berezowska, Irena
    Lemieux, Carole
    Chung, Nga
    Zelent, Bogumil
    Schiller, Peter
    Powiązania:
    https://bibliotekanauki.pl/articles/1043324.pdf
    Data publikacji:
    2004
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    fluorescence spectroscopy
    opioid activity profile in vitro
    fluorescence quantum yield
    opioid peptides
    fluorescent opioid peptide analogues
    [Dmt1]DALDA
    Opis:
    Dansylated analogues of the potent and selective μ opioid peptide agonist [Dmt1]DALDA (H-Dmt-D-Arg-Phe-Lys-NH2; Dmt = 2',6'-dimethyltyrosine) were prepared either by substitution of Nβ-dansyl-α,β-diaminopropionic acid or Nε-dansyllysine for Lys4, or by attachment of a dansyl group to the C-terminal carboxamide function via a linker. All three analogues displayed high μ agonist potency in vitro and the C-terminally dansylated one retained significant μ receptor selectivity. The three analogues showed interesting differences in their fluorescence emission maxima and quantum yields, indicating that the dansyl group in two of them was engaged in intramolecular hydrophobic interactions. These dansylated [Dmt1]DALDA analogues represent valuable tools for binding studies, cellular uptake and intracellular distribution studies, and tissue distribution studies.
    Źródło:
    Acta Biochimica Polonica; 2004, 51, 1; 107-113
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Co-operation between particulate and soluble guanylyl cyclase systems in the rat renal glomeruli
    Autorzy:
    Stepinski, J.
    Wendt, U.
    Lewko, B.
    Angielski, S.
    Powiązania:
    https://bibliotekanauki.pl/articles/70751.pdf
    Data publikacji:
    2000
    Wydawca:
    Polskie Towarzystwo Fizjologiczne
    Tematy:
    nitric oxide
    blood flow
    kidney
    atrial natriuretic peptide
    cardiovascular system
    glomerular filtration
    rat
    glomerulus
    renal glomerulus
    guanylyl cyclase system
    Źródło:
    Journal of Physiology and Pharmacology; 2000, 51, 3
    0867-5910
    Pojawia się w:
    Journal of Physiology and Pharmacology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Genetic variants of the glucagon-like receptor-1 in obesity
    Autorzy:
    Nikulina, Anna
    Powiązania:
    https://bibliotekanauki.pl/articles/29519596.pdf
    Data publikacji:
    2023-12-31
    Wydawca:
    Uniwersytet Rzeszowski. Wydawnictwo Uniwersytetu Rzeszowskiego
    Tematy:
    analysis of single nucleotide gene variants
    children
    glucagon-like peptide-1 receptor
    metabolically healthy obesity
    metabolically unhealthy obesity
    Opis:
    Introduction and aim. Dysfunction of the glucagon-like peptide 1 (GLP-1)/GLP-1 receptor (GLP-1R) axis promotes obesity and metabolic disorders. The aim was to study the associations of the single nucleotide variants (SNV) GLP1R gene with proinflammatory cytokines and metabolic disorders in children with various obesity phenotypes. Material and methods. 252 children with obesity aged 6-18 years were examined. The first group (n=152) was represented by children with metabolically unhealthy obesity (MUO). The second group (n=100) consolidated of children with metabolically healthy obesity (MHO). Whole genome sequencing (CeGat, Germany) was performed in 52 children. Results. An association with the development of obesity was noted for T alleles rs61754624 (t=3.33) and rs10305457 (t=2.06); with MUO – for C alleles rs1042044 (t=2.23), rs1126476 (t=2.63), rs2235868 (t=2.82); T alleles rs61754624 (t=3.33), rs10305457 (t=2.06) GLP1R, p<0.05. In the MHO group, a correlation was found with the levels of pro-inflammatory markers IL-1β, IL-6 in the presence of the GA genotype SNV rs3765468; with hyperglycemia - GA genotype SNV rs6923761, CC genotype SNV rs1042044, AA rs6918287; hyperinsulinemia - GA genotype SNV rs3765468, GG rs10305421; triglyceridemia - AA rs6918287 of GLP1R. Conclusion. SNV rs1042044, rs3765468, rs6923761, s6918287, and rs rs10305421 GLP1R are associated with the development of MUO in individuals with MHO.
    Źródło:
    European Journal of Clinical and Experimental Medicine; 2023, 4; 682-691
    2544-2406
    2544-1361
    Pojawia się w:
    European Journal of Clinical and Experimental Medicine
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Conformational investigation of α,β-dehydropeptides. XIII. Conformational properties of N-acetyl-α,β-dehydrovaline N',N'-dimethylamide.
    Autorzy:
    Siodłak, Dawid
    Rzeszotarska, Barbara
    Broda, Małgorzata
    Kozioł, Anna
    Kołodziejczyk, Edyta
    Powiązania:
    https://bibliotekanauki.pl/articles/1043332.pdf
    Data publikacji:
    2004
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    ab initio/DFT calculations
    N',N'-dimethylamides
    X-ray crystallography
    α,β-dehydroamino acids
    peptide design
    valine derivative
    Opis:
    The crystal structure of Ac-ΔVal-NMe2 (ΔVal = α,β-dehydrovaline) was determined by X-ray crystallography. The found angles φ = -60° and ψ = 125° correspond exactly to the respective values of the (i + 1)th residue in idealised β-turn II/VIa. Ab initio/DFT studies revealed that the molecule adopts the angle ψ restricted only to about |130°| and very readily attains the angle φ = about -50°. This is in line with its solid-state conformation. Taken together, these data suggest that the ΔVal residue combined with a C-terminal tertiary amide is a good candidate at the (i + 1)th position in a type II/VIa β-turn.
    Źródło:
    Acta Biochimica Polonica; 2004, 51, 1; 145-152
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Identyfikacja białek z wykorzystaniem techniki Peptide Mass Fingerprinting (PMF). Część II - algorytmy scoringu
    The identification of proteins by Peptide Mass Fingerprinting (PMF). Part II - the scoring algorithms
    Autorzy:
    Kamińska, H.
    Podbielska, H.
    Powiązania:
    https://bibliotekanauki.pl/articles/261443.pdf
    Data publikacji:
    2011
    Wydawca:
    Politechnika Wrocławska. Wydział Podstawowych Problemów Techniki. Katedra Inżynierii Biomedycznej
    Tematy:
    proteomika
    identyfikacja protein
    spektrometria masowa
    peptide mass fingerprinting
    schematy scoringu
    proteomics
    identification of proteins
    mass spectrometry
    scoring schemes
    Opis:
    Postęp w dziedzinie komputerów oraz rozwój Internetu zrewolucjonizował, proces identyfikacji białek oraz przyczynił się do szybkiego wzrostu proteomicznych baz danych. Krótko po wprowadzeniu pierwszej technologii identyfikacji białek z widm spektrometrów masowych PMF (Peptide Mass Fingerprinting) okazało się, że algorytmy wykorzystywane do wyszukiwania w bazie danych protein odpowiadających wynikom eksperymentu mają kluczowe znaczenie dla wysokiej poprawności identyfikacji. Rozwój metody PMF był zatem uwarunkowany nie tylko przez usprawnienia techniczne schematu, ale przede wszystkim przez zastosowanie rozmaitych metod matematycznych i statystycznych (tzw. algorytmów scoringu) przy wyszukiwaniu poprawnych rozwiązań. Kolejnym krokiem w informatycznym usprawnieniu identyfikacji było opracowanie metod walidacji jej rezultatów na podstawie istniejących baz danych lub też symulacji. Walidacja rezultatów pozwoliła na wyeliminowanie większości błędów pierwszego rodzaju w identyfikacji metodą PMF. Przez wzgląd na powszechność stosowania metody, a także jej ulepszenia autorzy postanowili podsumować obecny stan wiedzy w tym zakresie. Praca została podzielona na dwie części: w pierwszej przedstawiono opis historii powstania metody PMF wraz z charakterystyką jej części eksperymentalnej i opisem najpopularniejszych baz danych stosowanych przy identyfikacji, natomiast druga część jest poświęcona zagadnieniom algorytmicznym związanym z wyszukiwaniem w bazie danych protein najlepiej odzwierciedlających białko analizowane w próbce. Bioinformatyczne ujęcie identyfikacji białek w drugiej części nawiązuje do specyfikacji eksperymentu, omówionej w części pierwszej publikacji. Druga część pracy w szczegółowy sposób opisuje główne aspekty porównywania mas teoretycznych i eksperymentalnych, tj. trawienie in silico, rozpoznawanie modyfikacji białek, dopasowywanie mas oraz kalibrację poprawnych dopasowań. Opisane zostały także sposoby budowania funkcji scoringowych oraz algorytmy walidacji ich wartości. Dodatkowo, w pracy przedstawiono najbardziej znane funkcje scoringowe oraz pełny przegląd oprogramowania do identyfikacji białek metodą PMF.
    The internet and computer science progress have revolutionized the process of protein identification and contributed to the growth of proteomics databases. Just after discovering the first technology for protein identification from the mass spectra PMF (peptide mass fingerprinting), it appeared that the algorithms searching databases for proteins corresponding to experiment results have crucial meaning for the sensitivity and specificity of the identification procedure. Therefore, the development of PMF method was conditioned by both the technological improvements in the PMF scheme and the application of various mathematical and statistical methods (so called: scoring algorithms) to the searching of correct identifications. The next step in the development of an identification procedure was to work out the methods for identification results validation, according to the proteomics databases content or simulations. The results validation allowed to eliminate the most of unwanted false positives in the PMF identification. Regarding the method common use, as well as its improvements which are still present, the authors decide to summarize the current level of knowledge related to this topic. The publication is divided into two parts. The first one is devoted to the origins of PMF scheme, the characteristics of its experimental part and a description of the most popular databases used in the identification procedure. The second part relates to the algorithmic issues of searching the database protein, which reflects the sample content best. From the bioinformatics point of view the protein identification in the second part of publication refers to the experiment specification described in the first part. The second part of the publication describes in details the aspects of theoretical and experimental masses comparison, i.e. in silico digestion, the discrimination of protein modifications, the pairing of masses and the calibration of matches. Moreover, the scoring functions building manners and the algorithms for scoring functions values validation were also taken into the consideration. Additionally, we present the most known scoring schemes with the comprehensive review of the PMF protein identification software.
    Źródło:
    Acta Bio-Optica et Informatica Medica. Inżynieria Biomedyczna; 2011, 17, 3; 239-247
    1234-5563
    Pojawia się w:
    Acta Bio-Optica et Informatica Medica. Inżynieria Biomedyczna
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Gamma-secretase complex in plant cells
    Autorzy:
    Skrzypczak, T.
    Smolarkiewicz, M.
    Wojtaszek, P.
    Powiązania:
    https://bibliotekanauki.pl/articles/80341.pdf
    Data publikacji:
    2013
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    conference
    gamma-secretase
    beta-amyloid peptide
    beta-amyloid precursor protein
    proteolysis
    calcium homeostasis
    apoptosis
    synapse
    intercellular junction
    Arabidopsis
    plant cell
    Źródło:
    BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2013, 94, 3
    0860-7796
    Pojawia się w:
    BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Zastosowanie metod elektrochemicznych w bioanalityce : wybrane zagadnienia
    Electrochemical methods in bioanalytics : selected aspects
    Autorzy:
    Ufnalska, I.
    Wiloch, M. Z.
    Wesoły, M.
    Ćwik, P.
    Zabadaj, M.
    Ciosek, P.
    Wawrzyniak, U. E.
    Wróblewski, W.
    Powiązania:
    https://bibliotekanauki.pl/articles/171732.pdf
    Data publikacji:
    2015
    Wydawca:
    Polskie Towarzystwo Chemiczne
    Tematy:
    elektrochemia
    elektroniczny język
    chemometria
    kompleksy peptydjony miedzi(II)
    warstwy boroorganiczne
    electrochemistry
    electronic tongue
    chemometrics
    peptide-copper(II) complexes
    boroorganic layers
    Opis:
    The cooperation of scientists specializing in different fields has given rise to the integration of previously distinct thematic areas and creation of new multidimensional disciplines as a result. Biochemistry, which has derived from the borderline of chemistry and biology, can be set as a good example. In this short review an insight into electrochemical studies, which are currently carried out in the Department of Microbioanalytics at the Faculty of Chemistry (Warsaw University of Technology), was presented. Three independent scientific pathways introducing electrochemical methods for biochemical and bioanalytical purposes can be distinguished among the ongoing researches. The first one embraces the design of the so-called electronic tongue – a system used for the qualitative and quantitative analysis of liquid samples of complex composition. In this work, potentiometric sensor arrays were applied to develop an electronic tongue system enabling the evaluation of the effectiveness of bitter taste masking of pharmaceuticals. The second scientific pathway involves voltammetric studies of the interactions of biologically active peptides with copper(II) ions. The interest was drawn to clarify and describe the role of β-amyloid and NSFRY copper(II) complexes, relevant to Alzheimer’s disease occurrence and cardiovascular system disorder respectively. Finally, boronic acids and their derivatives, exhibiting the affinity for molecules possessing 1,2 or 1,3-diol group in their structure, were used as selective molecular receptors in the third research project. The studies include the selection of the optimal method and conditions of the immobilization process, providing the most favorable receptor layer structure, and the determination of the performances of constructed electrochemical sensor towards particular bioanalytes.
    Źródło:
    Wiadomości Chemiczne; 2015, 69, 9-10; 931-946
    0043-5104
    2300-0295
    Pojawia się w:
    Wiadomości Chemiczne
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Metoda O-acyloizopeptydowa w syntezie peptydow
    O-acyl isopeptide method in peptide synthesis
    Autorzy:
    Frączak, O.
    Olma, A.
    Powiązania:
    https://bibliotekanauki.pl/articles/171738.pdf
    Data publikacji:
    2014
    Wydawca:
    Polskie Towarzystwo Chemiczne
    Tematy:
    peptide
    O-acyloizopeptydy
    O N-migracja
    peptydy
    peptydy o trudnych sekwencjach
    O-acyloisopeptides
    O N-migration
    difficult sequence containing peptides
    Opis:
    Proteins are macromolecules that carry out most of the biochemical functions of the cell, which strongly depend on the secondary and tertiary structure, defined by the amino acid sequence of a polypeptide chain. The importance of peptides and proteins in biology and medicine inspired chemists to develop strategies for their synthesis. The main limitation to the preparation of long peptides is their tendency to aggregation, what makes the coupling and deprotection reaction ineffective, and purification of the compounds difficult. Inter- and intramolecular interactions, hydrophobic character, the presence of multiple hydrogen bonds significantly affect the secondary structure of peptides, making further extension of the peptide chain very difficult. Undesirable aggregation process may be disrupted by reduction of hydrophobic interactions. For this purpose, various methods are used, based on the implementation of specific modifications to the peptide chain, affecting its secondary structure. These methods include, for example, incorporation of pseudoproline building blocks [5] and proximity induced peptide ligation [6, 7]. In some cases, it is convenient to extend the amino acid side chain to form isopeptides (Fig. 1) [14–16]. Depsipeptides can be created with the natural amino acids such as cysteine, serine, threonine, tyrosine, or tryptophan. The basic requirement is the presence of β-hydroxyamino component. The presence of a depsipeptide moiety in place of an amide bond significantly change the secondary structure of native peptide and prevents from aggregation, leading to higher yields of desired compounds [18]. In the solution phase peptide synthesis, this method is free from racemization [19]. Isodipeptide units can be successfully applied in SPPS for the synthesis of “difficult sequence”-containing peptides [19]. In this paper, many examples of effective use of O-acylisopeptides method in peptide synthesis are discussed.
    Źródło:
    Wiadomości Chemiczne; 2014, 68, 7-8; 733-749
    0043-5104
    2300-0295
    Pojawia się w:
    Wiadomości Chemiczne
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    trans-Pro isosteres in the development of non-selective and selective mimetic agonists of insect pyrokinin neuropeptides: A review
    Autorzy:
    Nachman, R.J.
    Powiązania:
    https://bibliotekanauki.pl/articles/55206.pdf
    Data publikacji:
    2009
    Wydawca:
    Sieć Badawcza Łukasiewicz - Instytut Przemysłu Organicznego
    Tematy:
    diapause hormone
    physiological process
    melanization
    pupariation
    pheromonotropin
    neuropeptide
    pyrokinin family
    pheromone
    diapause break
    pheromone biosynthesis activating neuropeptide
    insect
    reddish colouration hormone
    peptide
    Opis:
    The pyrokinin (PK) family plays a multifunctional role in an array of important physiological processes in a variety of insects. A PK active core analog containing an (E)-alkene, transPro isosteric component was evaluated in five disparate PK bioassays and/or in a recombinant PK receptor cell line, representing six different insect species. The assays included pheromone biosynthesis in the moth Heliothis peltigera, melanization in the larval Spodoptera littoralis, pupariation acceleration in the larval fly Neobellieria bullata, diapause termination in the moth Heliothis zea, and hindgut contraction in the cockroach Leucophaea maderae. This constrained analog demonstrated unselective agonist activity that approached, matched, or exceeded the activity of parent PK peptides of equal length in all six PK assays. The results provide strong evidence for the orientation of Pro and the core conformation adopted by PK neuropeptides during interaction with disparate PK receptors. A PK active core analog incorporating a second transPro motif, the dihydroimidazoline moiety, was found to demonstrate pure, selective agonism in the melanotropic bioassay, with no significant activity in three other PK bioassays. Both types of transPro isosteric analogs feature modification adjacent to the primary tissue-bound peptidase hydrolysis site that is expected to enhance biostability over natural PK peptides. The research further identifies two novel scaffolds with which to design either selective or non-selective mimetic PK analogs as potential leads in the development of environmentally favorable pest management agents capable of disrupting PK-regulated systems.
    Źródło:
    Pestycydy; 2009, 1-4; 33-39
    0208-8703
    Pojawia się w:
    Pestycydy
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    A brief overview of the process of the elucidation of GnRH structure (1971)
    Autorzy:
    Kochman, K.
    Powiązania:
    https://bibliotekanauki.pl/articles/80024.pdf
    Data publikacji:
    2012
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    luteinizing hormone
    follicle stimulating hormone
    receptor protein
    protein G
    cell membrane
    hypothalamic peptide
    adrenocorticotrophin
    radioimmunoassay
    central nervous system
    gonadotrophin releasing hormone
    receptor
    neuroendocrinology
    Źródło:
    BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2012, 93, 4
    0860-7796
    Pojawia się w:
    BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    The cell-free protein biosynthesis - applications and analysis of the system.
    Autorzy:
    Lamla, Thorsten
    Mammeri, Kerstin
    Erdmann, Volker
    Powiązania:
    https://bibliotekanauki.pl/articles/1044140.pdf
    Data publikacji:
    2001
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    in vitro protein biosynthesis
    ribosome display
    2D-gel elctrophoresis
    His-tag and function
    in vitro evolution of proteins
    Strep-tag affinity peptide
    Opis:
    The in vitro protein biosynthesis has the potentials to become a powerful technology for biochemical research. Beside the determination of structure and function the in vitro evolution of proteins is also of great interest. The system described was used to produce bovine heart fatty acid binding protein (FABP) and bacterial chloramphenicol acetyltransferase (CAT) with and without fusion of the Strep-tag II affinity peptide. The proteins were purified after and during protein biosynthesis by using a StrepTactin Sepharose matrix. No significant influence of the Strep-tag and the conditions during the affinity chromatography on maturation or activity of the protein was observed. The in vitro evolution of proteins is feasible by means of ribosome display. The selection of a specific mRNA coding for a shortened FABP with a N-terminal His-tag via the accompanying protein property was shown. Goal of the selection was to bind the FABP via the His-tag on Ni(II)-IDA-agarose. After nine cycles of transcription, translation, affinity selection and RT-PCR the protein with the His-tag could be enriched 108-fold. In order to correlate a possible relationship between changes in protein population and biological function studies were initiated in which 2-dimensional protein patterns of the total in vitro system were compared after 0 and 2 h reaction time. The very interesting findings are that a number of proteins disappear, while others are newly formed during protein synthesis.
    Źródło:
    Acta Biochimica Polonica; 2001, 48, 2; 453-465
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Polymers for peptide/protein arrays
    Polimery w macierzach peptydowych/białkowych
    Autorzy:
    Szweda, R.
    Lipowska, D.
    Silberring, J.
    Dworak, A.
    Trzebicka, B.
    Powiązania:
    https://bibliotekanauki.pl/articles/945832.pdf
    Data publikacji:
    2015
    Wydawca:
    Sieć Badawcza Łukasiewicz - Instytut Chemii Przemysłowej
    Tematy:
    peptide arrays
    protein arrays
    polymeric surfaces
    microarrays
    polymeric linkers
    poly(ethylene glycol)
    dendrimers
    macierze peptydowe
    macierze białkowe
    powierzchnie polimerowe
    mikromacierze
    łączniki (linkery) polimerowe
    poli(glikol etylenowy)
    dendrymery
    Opis:
    Peptide and protein arrays have gained increasing attention due to their potential application in many areas of research, clinical diagnosis, and pharmacy. A typical array consists of asupport containing immobilized peptides or proteins positioned in an addressable format. The greatest advantage of the arrays is the possibility for miniaturization, which relies on dividing the surface into miniature spots, thus allowing for hundreds/thousands of analyses to be simultaneously performed using minimal amounts of aprecious biological material. The quality of assays with the use of peptide and protein arrays depends on the surface properties, e.g., hydrophilicity, homogeneity, density of functional groups, surface morphology, etc. In recent years, it was shown that the quality of the assays might be improved by introducing polymers acting as spacers between the peptide and the solid support. This approach causes changes in the surface properties, e.g., it reduces the undesirable non-specific adsorption of biomolecules, increases the density of functional groups, or can improve the biological activity of biomolecules attached to the surface. In this review, various types of polymers that are used for peptide and protein arrays and their impact on the assay quality are discussed.
    Peptydy lub białka naniesione w regularnych, uporządkowanych pozycjach na nośnik stały tworzą tzw. macierze. Układy takie wzbudzają coraz większe zainteresowanie, ponieważ można je wykorzystywać do prowadzenia analiz w biochemii, diagnostyce klinicznej czy farmacji. Największą zaletą macierzy jest możliwość miniaturyzacji. Podział powierzchni macierzy na mikroplamki (mikrospoty) pozwala na wykonywanie do kilkuset analiz jednocześnie z wykorzystaniem minimalnej ilości cennego materiału biologicznego. Jakość analiz przeprowadzanych przy użyciu macierzy peptydowych i białkowych zależy od takich właściwości powierzchni, jak: hydrofilowość, jednorodność, gęstość obsadzenia grupami funkcyjnymi, morfologia, itp. W ostatnich latach wykazano, że można poprawić jakość analiz w wyniku wprowadzenia polimerów między peptyd/białko a podłoże. Polimery zmieniają właściwości powierzchni macierzy, np. redukują niepożądaną adsorpcję biocząsteczek, zwiększają gęstość obsadzenia powierzchni grupami funkcyjnymi lub poprawiają dostępność biocząsteczek związanych z powierzchnią. W niniejszej pracy omówiono różne typy polimerów stosowane do otrzymywania macierzy peptydowych i białkowych oraz ich wpływ na jakość przeprowadzanych analiz.
    Źródło:
    Polimery; 2015, 60, 2; 75-86
    0032-2725
    Pojawia się w:
    Polimery
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Biwalentne ligandy receptorów opioidowych
    Bivalent ligands of opioid receptors
    Autorzy:
    Frączak, O.
    Olma, A.
    Powiązania:
    https://bibliotekanauki.pl/articles/171626.pdf
    Data publikacji:
    2014
    Wydawca:
    Polskie Towarzystwo Chemiczne
    Tematy:
    peptydy opioidowe
    biwalentne ligandy niepeptydowe
    biwalentne ligandy peptydowe
    aktywność opioidowa
    homobiwalentne ligandy
    heterobiwalentne ligandy
    opioid peptides
    nonpeptide bivalent ligands
    peptide bivalent ligands
    opioid activity
    homobivalent ligands
    heterobivalent ligands
    Opis:
    Opioids are the oldest drugs know to humanity, which have been and continue to be used for the treatment of chronic pain. Unfortunately they have a large numbers of side effects [1–6]. Three main types of opioid receptors μ (MOR), δ (DOR) and κ (KOR) are known [8]. The ORL1 receptor was classified as the fourth member of opioid receptor family [9]. Opioid receptors can form homodimers and the following heterodimers: DOR-KOR, DOR-MOR and KOR-MOR [13c,d,f, 14]. Specially designed ligands which are able to penetrate the BBB are used to study physiological consequences of opioid receptor homo- and heterodimerization, and as new analgesics. Bivalent ligands are defined as compounds that contain two pharmacophoric units, an appropriately designed spacer to separate and define the two pharmacophores, and a linker unit to connect the pharmacophores, to the spacer (Fig. 1) [16]. The affinity of a ligand to its target depends on its fundamental kinetic association and dissociation rate constants (Scheme 1) [24]. Bivalent ligands interacting with the opioid receptors have been divided into three groups: nonpeptide, peptide- nonpeptide and peptide homo- or heterodimers. Nonpeptide bivalent ligands (4–21, 27–41 and 44–45) containing different pharmacophores (selective opioid agonists or/and antagonists) connected with designed linkers have potent analgesic properties [25–34]. Compound 35 may be useful in the treatment of opioid dependence. Studies of peptide-nonpeptide ligands, which are a combination of “address” segments of endogenous opioid peptides and selective alkaloid ligand (47–50) indicate that peptide part of the analogues can modulate the receptor selectivity of the attached alkaloid pharmacophores [35]. Series of peptide-nonpeptide ligands containing different classes of opioid peptides and fentanyl (52–86) were synthesized and tested for binding affinity to μ and δ opioid receptors [38–40]. Good opioid affinity and antinociceptive activity of some of the obtained bivalent ligands (57, 61, 63) suggesting that a novel class of analgesics can be further developed utilizing this approach. Among homobivalent ligands the most important is biphalin 87 and its analogues (88–124) [41–53]. Analgesic potency of the most active ligand 112 is greater than parent peptide (biphalin) and morphine.
    Źródło:
    Wiadomości Chemiczne; 2014, 68, 3-4; 233-255
    0043-5104
    2300-0295
    Pojawia się w:
    Wiadomości Chemiczne
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Oznaczanie gliadyny w niektorych produktach bezglutenowych metoda Elisa
    Gliadine determination in some gluten-free products by Elisa methods
    Autorzy:
    Maslowska, J
    Leszczynska, J.
    Powiązania:
    https://bibliotekanauki.pl/articles/871921.pdf
    Data publikacji:
    1992
    Wydawca:
    Narodowy Instytut Zdrowia Publicznego. Państwowy Zakład Higieny
    Tematy:
    surowce roslinne
    prolaminy
    zywnosc
    gliadyna
    zboza
    peptydy
    oznaczanie
    metody immunoenzymatyczne
    gluten
    produkty bezglutenowe
    plant raw material
    prolamin
    food
    gliadin
    cereal
    peptide
    determination
    immunoenzymatic method
    gluten-free product
    Opis:
    Zastosowano z pomyślnym rezultatem metodę immunoenzymatyczną z wykorzystaniem króliczych przeciwciał przeciwko gliadynie i koniugatów przeciwciał z peroksydazą do oznaczania gliadyny w bezglutenowej żywności. Otrzymane wyniki oznaczania gliadyny wskazują, że zawartość gliadyny jest w nich niska i nie przekracza 0,1%.
    Immunoenzymatic method with rabbit antibodies against gliadine and antibodies conjugates with peroxidase for gliadine determination in gluten-free food using polipropylene and polistyrene was succesfully used. Results of gliadine determination indicate that content of gliadine in investigated samples was low - about 0,1%.
    Źródło:
    Roczniki Państwowego Zakładu Higieny; 1992, 43, 1; 61-66
    0035-7715
    Pojawia się w:
    Roczniki Państwowego Zakładu Higieny
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Cathelicidin LL-37, granzymes, TGF-beta1 and cytokines levels in induced sputum from farmers with and without COPD
    Autorzy:
    Golec, M
    Reichel, C.
    Mackiewicz, B.
    Skorska, C.
    Curzytek, K.
    Lemieszek, M.
    Dutkiewicz, J.
    Gora, A.
    Ziesche, R.
    Boltuc, J.
    Sodolska, K.
    Milanowski, J.
    Spiewak, R.
    Powiązania:
    https://bibliotekanauki.pl/articles/49729.pdf
    Data publikacji:
    2009
    Wydawca:
    Instytut Medycyny Wsi
    Tematy:
    cathelicidin LL-37
    peptide
    chronic obstructive pulmonary disease
    COPD zob.chronic obstructive pulmonary disease
    sputum
    farmer
    human disease
    organic dust
    ELISA test
    spirometric value
    sputum sample
    dust exposure
    cytokine
    lipopolysaccharide
    Źródło:
    Annals of Agricultural and Environmental Medicine; 2009, 16, 2; 289-297
    1232-1966
    Pojawia się w:
    Annals of Agricultural and Environmental Medicine
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Badanie struktury i dynamiki N-terminalnych sekwencji dermorfiny i ich analogów z wykorzystaniem spektroskopii NMR w ciele stałym i rentgenografii
    Studies on the structure and dynamics of N-terminal sequences of dermorphin and their analogs by means of solid state NMR spectroscopy and XRD
    Autorzy:
    Trzeciak-Karlikowska, K.
    Powiązania:
    https://bibliotekanauki.pl/articles/171648.pdf
    Data publikacji:
    2012
    Wydawca:
    Polskie Towarzystwo Chemiczne
    Tematy:
    peptydy opioidowe
    oddziaływania CH-pi
    dynamika molekularna
    oddziaływanie peptyd–fosfolipid
    spektroskopia NMR
    1H Ultra Fast MAS NMR
    PISEMA MAS
    PILGRIM
    XRD
    opioid peptides
    CH-pi interactions
    molecular dynamics
    peptide–phospholipid interaction
    NMR spectroscopy
    Opis:
    Deltorphin I (Tyr-d-Ala-Phe-Asp-Val-Val-Gly-NH_2) and dermorphin (Tyr-d-Ala-Phe- -Gly-Tyr-Pro-Ser-NH_2) are natural opioid peptides that have been isolated from the skin of South American frogs [1]. The presence of d-amino acid is crucial for their biological activity. The synthetic analogs of given heptapeptides containing l-alanine are not analgesics [2]. Analysis of the influence of stereochemistry on molecular packing, dynamics and biological functions of neuropeptides is still important for receptor studies and practical applications (e.g. design of new selective pain killers). Presented research is focused on the structure and dynamics of two N-terminal sequences of dermorphin: tripeptide Tyr-d-Ala-Phe 1, tetrapeptide Tyr-D-Ala-Phe-Gly 2, and their analogs with l-alanine: Tyr-Ala-Phe 3 and Tyr-Ala-Phe-Gly 4, using solid state NMR and X-ray diffraction. This study clearly demonstrates that 1 and 2 crystallized under different conditions to form exclusively one structure [3, 4]. In contrast, tripeptide and tetrapeptide with l-Ala in the sequence very easily form different crystal modifications. Tyr-Ala-Phe 3 crystallizes into two forms: 3a and 3b [5], while Tyr-Ala-Phe-Gly 4 gives three modifications: 4a, 4b and 4c [4]. It seems that one of the factors, which can be important in the preorganization mechanism anticipating the formation of crystals, is the intramolecular CH-đ interaction between aromatic rings of tyrosine and/or phenylalanine and the methyl group of alanine. Such interaction is possible only for d-Ala residue. For l-Ala in the peptide sequence, the methyl group is aligned on the opposite side with respect at least to one of the aromatic groups. It can be further speculated that such internal CH-π contacts can also occur during the interaction of ligand–receptor, making the message sequence of opioid peptides more rigid and finally selective. By employing different NMR experiments (e.g. PISEMA MAS and PILGRIM) it was proven that the main skeleton of analyzed peptides is rigid, whereas significant differences in the molecular motion of the aromatic residues were observed [4, 6]. Solid state 2H NMR spectroscopy of samples with deuterium labeled aromatic rings: Tyrd4-d-Ala-Phe 5, Tyr-d-Ala-Phed5 6, Tyrd4-Ala-Phe 7, Tyr-Ala-Phe^d5 8 was used to analyze the geometry and time scale of the molecular motion. At ambient temperature, the tyrosine ring of sample 5 is rigid and in the sample 6 the phenylalanine ring undergoes a "π -flip". The tyrosine rings of form I of 7 and 8 are static, while the phenylalanine rings of form II of 7 and 8 undergo a fast regime exchange [6]. Variable temperature 2H measurements proved that the tyrosine and phenylalanine rings of two forms of compounds 7 and 8 became more mobile with increasing temperature. In contrast, the aromatic rings of samples 5 and 6 preserve their dynamics regime (static tyrosine and "π -flip" phenylalanine) in a large range of temperatures [6]. The analysis of 13C, 15N labeled tetrapeptide Tyr-D-Ala-Phe-Gly 2’-phospholipid membrane interactions suggests that peptide 2’ is aligned on the surface of the membrane (RFDR MAS) and the sandwich-like π -CH_3-π arrangement of the pharmacophore is preserved (DARR) [7].
    Źródło:
    Wiadomości Chemiczne; 2012, 66, 9-10; 867-891
    0043-5104
    2300-0295
    Pojawia się w:
    Wiadomości Chemiczne
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    The role of binary and ternary systems in protein studies
    Rola systemów binarnych I ternarnych w badaniu białek
    Autorzy:
    Ławrynowicz, Julian
    Nowak-Kępczyk, Małgorzata
    Suzuki, Osamu
    Powiązania:
    https://bibliotekanauki.pl/articles/1837641.pdf
    Data publikacji:
    2021-08-12
    Wydawca:
    Łódzkie Towarzystwo Naukowe
    Tematy:
    binary physical structure
    ternary physical structure
    quaternary physical structure
    quinary physical structure
    senary physical structure
    alloy
    pentacene
    polymer
    protein
    peptide
    amino acid
    Galois extension
    Riemann surface
    binarna struktura fizyczna
    ternarna struktura fizyczna
    kwaternarna struktura fizyczna
    kwinarna struktura fizyczna
    sennarna struktura fizyczna
    stop
    pentacen
    polimer
    białko
    peptyd
    aminokwas
    rozszerzenie Galois
    powierzchnia Riemanna
    Opis:
    Various aspects of binary, ternary, quaternary, quinary, and senary structures for alloys, polymers and, in particular, proteins are studied. We refer to quinary and senary structures in some polymers indicating the role of total energy maxima in the infrared and Raman activity energy spectra. Decomposition of quinary structures to ternary structures is discussed. A complex analytical method of binary and ternary Galois extension is proposed as well as its realization in terms of Riemann surfaces. Slightly wavy behaviour of the system of hexagons in a polymer leaf is investigated
    Rozważamy rozmaite aspekty struktur binarnych, ternarnych, kwaternarnych i senarnych dla stopów, polimerów i protein. W szczególności odnosimy się do struktur kwinarnych i senarnych w niektórych polimerach wskazując na rolę maksimów energii w spektrach podczerwieni i aktywności Ramana. Dyskutujemy rozkład struktur kwinarnych do ternarnych. Proponujemy zespoloną metodę analityczną dla binarnych i ternarnych rozszerzeń Galois, jak również ich realizację na powierzchniach Riemanna. Omawiamy lekko falujące zachowanie układu sześciokątów w liściu polimeru
    Źródło:
    Bulletin de la Société des Sciences et des Lettres de Łódź, Série: Recherches sur les déformations; 2020, 70, 1; 169-187
    1895-7838
    2450-9329
    Pojawia się w:
    Bulletin de la Société des Sciences et des Lettres de Łódź, Série: Recherches sur les déformations
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Aminokwasy, glikany, peptydy i białka w ścieżkach diagnostycznych i terapeutycznych chorób cywilizacyjnych XXI wieku : projektowanie i charakterystyka fizykochemiczna oraz strukturalna
    Amino acids, glycans, peptides and proteins in the diagnostic and therapeutic pathways of the 21st century civilization diseases : design, physicochemical and structural characterisation
    Autorzy:
    Bylińska, Irena
    Dzierżyńska, Maria
    Giżyńska, Małgorzata
    Guzow, Katarzyna
    Jankowska, Elżbieta
    Jurczak, Przemysław
    Kaczyński, Zbigniew
    Karska, Natalia
    Kowalczyk, Agnieszka
    Kuncewicz, Katarzyna
    Orlikowska, Marta
    Sawicka, Justyna
    Spodzieja, Marta
    Szpakowska, Nikola
    Szymańska, Aneta
    Wieczerzak, Ewa
    Witkowska, Julia
    Rodziewicz-Motowidło, Sylwia
    Powiązania:
    https://bibliotekanauki.pl/articles/2200549.pdf
    Data publikacji:
    2022
    Wydawca:
    Polskie Towarzystwo Chemiczne
    Tematy:
    fluorophores
    fluorescence spectroscopy
    antimicrobial activity
    anticancer activity
    Cystapep
    Stahylococcus aureus
    antimicrobial compounds
    amyloidogenic protein
    mutagenesis
    fibrilization
    proteasome
    aging
    neurodegeneration
    self-assembling peptides
    tissue engineering
    biomaterials
    immune checkpoints
    peptide inhibitors
    immunotherapy
    ligands of TAP protein
    viral diseases
    NMR structure of the UL49.5 protein
    glycans
    glycoconjugates
    fluorofory
    spektroskopia fluorescencyjna
    aktywność przeciwdrobnoustrojowa
    aktywność antynowotworowa
    Staphylococcus aureus
    związki przeciwbakteryjne
    białko amyloidogenne
    mutageneza
    fibrylizacja
    proteasom
    procesy starzeniowe
    neurodegeneracja
    peptydy samoorganizujące
    inżynieria tkankowa
    biomateriały
    punkty kontrolne układu immunologicznego
    inhibitory peptydowe
    immunoterapia
    ligandy białka TAP
    choroby wirusowe,
    struktura NMR białka UL49.5
    glikany
    glikokoniugaty
    Opis:
    The civilization diseases of the 21st century are non-infectious disorders, affecting a large part of modern society. They are associated with the significant development of industry and technology, and hence with environmental pollution and an unhealthy lifestyle. These factors have led to the development of many civilization diseases, which currently include: cardiovascular diseases, respiratory diseases, diabetes, obesity, malignant tumors, gastrointestinal diseases, mental disorders and allergic diseases. The development of technologies, including modern therapies and new drugs, resulted in increase in life expectancy. This creates a global problem of an aging population with an increasing number of diseases of the old age, i.e. dementias. In addition, sedentary lifestyles and changing diets are the reasons why more and more people develop metabolic diseases, as well as neurological and cognitive disorders characterized by progressive damage to nerve cells and dementia. Currently, problem on a global scale is also the growing resistance to existing antimicrobial drugs. Therefore, the scientists face many challenges related to searching for the causes of these diseases, their diagnosis and treatment. Scientific research conducted at the Department of Biomedical Chemistry at the Faculty of Chemistry of the University of Gdańsk is part of this research trend. In this publication, we discuss various research topics with the long-term aim of solving the problems associated with the diseases mentioned above. The following chapters are dedicated to (i) looking for new effective fluorophores with diagnostic and anti-cancer activity; (ii) designing of new compounds with antibacterial and antiviral activity and their synthesis; (iii) investigating the mechanisms of amyloid deposit formation by human cystatin C and possibilities of inhibition of this process; (iv) designing and studies of compounds activating the proteasome with the potential to suppress the development of neurodegenerative diseases; (v) designing peptide fibrils and hydrogels as drug carriers; (vi) searching for peptide inhibitors of immune checkpoint as potential drugs for immunotherapy; (vii) studying the mechanism of action of selected herpesviruses by determining the structure of viral proteins and (viii) studying the composition of natural glycans and glycoconjugates in order to better understand the mechanisms of interaction of bacteria with the environment or with the host.
    Źródło:
    Wiadomości Chemiczne; 2022, 76, 5-6; 393--431
    0043-5104
    2300-0295
    Pojawia się w:
    Wiadomości Chemiczne
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
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