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Tytuł:
Application of gel filtration for rapeseed peptides analysis. Short report
Autorzy:
Amarowicz, R.
Kmita-Glazewska, H.
Powiązania:
https://bibliotekanauki.pl/articles/1372750.pdf
Data publikacji:
1995
Wydawca:
Instytut Rozrodu Zwierząt i Badań Żywności Polskiej Akademii Nauk w Olsztynie
Tematy:
gel filtration
peptide fraction
peptide separation
peptide
peptide extraction
food
chromatographic analysis
rapeseed
Źródło:
Polish Journal of Food and Nutrition Sciences; 1995, 04, 1; 88-92
1230-0322
2083-6007
Pojawia się w:
Polish Journal of Food and Nutrition Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Biologically active peptides derived from food proteins
Autorzy:
Kostyra, H.
Kostyra, E.
Powiązania:
https://bibliotekanauki.pl/articles/1371450.pdf
Data publikacji:
1992
Wydawca:
Instytut Rozrodu Zwierząt i Badań Żywności Polskiej Akademii Nauk w Olsztynie
Tematy:
man
peptide
biologically active peptide
natural peptide
human nutrition
nutrition
food
food protein
protein degradation
biotechnology
Źródło:
Polish Journal of Food and Nutrition Sciences; 1992, 01, 4; 5-17
1230-0322
2083-6007
Pojawia się w:
Polish Journal of Food and Nutrition Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The search for new biological activities for selected insect peptides
Autorzy:
Kuczer, M.
Dziubasik, K.
Luczak, M.
Majewska, A.
Kamysz, W.
Saniewska, A.
Konopinska, D.
Powiązania:
https://bibliotekanauki.pl/articles/55140.pdf
Data publikacji:
2008
Wydawca:
Sieć Badawcza Łukasiewicz - Instytut Przemysłu Organicznego
Tematy:
antitumour peptide
antifungal activity
antiviral peptide
oostatic peptide
antiviral activity
Phoma narcissi
Botrytis tulipae
colony growth
biological property
antimicrobial activity
insect peptide
biological activity
insect
peptide
Opis:
New biological properties of selected insect peptides are presented. The subjects of the investigation included insect oostatic peptides, like Neb-colloostatin (I) and Neb-TMOF(II), and/or insect peptides with antiviral or antitumor activity, such as alloferon (III) and its analogues modified at position 1 of the peptide chain. In the study was also included the oligopeptide Any-GS (VII) and its truncated analogues. The peptides were tested for antimicrobial activity on a series of bacterial species, antiviral activity against Human Herpes Virus type 1 (HHV-1) in vitro using a Vero cell line, and the growth and development of plant pathogens Phoma narcissi and Botrytis tulipae. The results of the biological investigations indicate that among the peptides investigated, compounds VII and IX inhibit the growth of plant pathogens P. narcissi and B. tulipae, whereas compounds I and II stimulate the mycelium growth of the aforementioned pathogens. Other peptides show slow antimicrobial activity but do not inhibit the replication of HHV-1 in Vero cells.
Źródło:
Pestycydy; 2008, 1-2; 5-11
0208-8703
Pojawia się w:
Pestycydy
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Structural biology of the influenza virus fusion peptide
Autorzy:
Worch, Remigiusz
Powiązania:
https://bibliotekanauki.pl/articles/1039239.pdf
Data publikacji:
2014
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
influenza virus
viral entry
membrane fusion
fusion peptide
peptide-lipid interactions
Opis:
The release of influenza RNA inside the host cell occurs through the fusion of two membranes, the viral envelope and that of the cellular endosome. The fusion is mediated by the influenza hemagglutinin protein (HA), in particular by the fusion peptide (HAfp) located in the N-terminal fragment of HA2 subunit. This protein fragment anchors in the internal endosomal membrane, whereas the C-terminal HA2 part comprises a transmembrane domain (TMD) embedded in the viral envelope. A drop of pH in the endosome acts as the main trigger for HA2 large conformational change that leads to anchoring of the fusion peptide, close contact of the membranes and the subsequent fusion. Throughout the years the major research effort was focused on a 20-aminoacid fragment (HAfp1-20), shown by NMR to adopt a 'boomerang'-like structure. However, recent studies showed that extending HAfp1-20 by three highly conserved residues W21-Y22-G23 leads to formation of a unique, tight helical hairpin structure. This review summarizes recently discovered structural aspects of influenza fusion peptides and their relations with the membrane fusion mechanism.
Źródło:
Acta Biochimica Polonica; 2014, 61, 3; 421-426
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
PRRT as an alternative method of treatment in patient with glucagonoma syndrome: A case report
Autorzy:
Popławska-Kita, A.
Szyszkowska, A.
Brelska, P.
Kowalczuk, M.
Szostek, A.
Popławski, Ł.
Siewko, K.
Szelachowska, M.
Kamiński, G.
Werel, D.
Górska, M.
Powiązania:
https://bibliotekanauki.pl/articles/1917699.pdf
Data publikacji:
2016
Wydawca:
Uniwersytet Medyczny w Białymstoku
Tematy:
Glucagonoma
tumor
peptide receptor radionuclide therapy
Opis:
Introduction: Glucagonoma is a rare pancreatic neuroendocrine tumor derived from alpha-cells of the islet of Langerhans. Due to oversecretion of glucagon it is associated with a characteristic paraneoplastic phenomenon, called glucagonoma syndrome, which consists of necrolytic migratory erythema (NME), weight loss, diabetes mellitus, diarrhea, normochromic normocytic anemia, deep vein thrombosis or pulmonary embolism and neuropsychiatric disturbances. Treatment modalities include surgical removal of tumor, somatostatin analogs and peptide receptor radionuclide therapy (PRRT). Case report: We present a case of 61-year-old woman diagnosed with glucagonoma in April 2012. Initially, body-caudal pancreatomy and resection of regional lymph nodes were performed. Five months after surgery, a PET-CT scan detected pathological mass with expression of somatostatin receptors in pancreatic body and metastases to regional lymph nodes. What is more, since April 2014 the patient had complained about persistent pruritus of the entire body. At present, due to the nonsurgical pancreatic mass and metastases she is treated with somatostatin analogs and PRRT. During this therapy the pruritus had decreased and currently there is no sign of cutaneous disease. Moreover, reduction of tumor size was obtained. Conclusions: PRRT may reduce tumor size and by reducing bothersome symptoms substantially improve the quality of life in patients with SSTR-positive tumors
Źródło:
Progress in Health Sciences; 2016, 6(1); 209-214
2083-1617
Pojawia się w:
Progress in Health Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Biological evaluation of analogues of an insect neuropeptide proctolin.
Autorzy:
Woźnica, Iwona
Szeszel-Fedorowicz, Wioletta
Rosiński, Grzegorz
Konopińska, Danuta
Powiązania:
https://bibliotekanauki.pl/articles/1043325.pdf
Data publikacji:
2004
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
proctolin analogues
proctolin
insect myotropic peptide proctolin
Opis:
Continuing our studies on proctolin (Arg-Tyr-Leu-Pro-Thr) we performed the synthesis and biological evaluation of 52 analogues substituted in position 2, 3, 4, and 5 of the peptide chain. The peptides were bioassayed for cardiotropic activity in vitro on Tenebrio molitor and myotropic activity on foregut of Schistocerca gregaria. Twenty analogues retained 20-80% of proctolin activity.
Źródło:
Acta Biochimica Polonica; 2004, 51, 1; 115-119
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Antibacterial peptides of the moth Galleria mellonella
Autorzy:
Mak, Paweł
Chmiel, Dorota
Gacek, Grzegorz
Powiązania:
https://bibliotekanauki.pl/articles/1044082.pdf
Data publikacji:
2001
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
antibacterial peptide
bacteriocin
insect immunity
hemolymph
cecropin
Opis:
The work describes purification and biochemical characterization of two inducible antimicrobial peptides from the hemolymph of Galleria mellonella. The peptides were isolated by a sequence of reversed-phase chromatography steps from the hemolymph of larvae immunized with viable bacteria. The first peptide is a member of the cecropin family while the second one is rich in proline residues and has a unique sequence.
Źródło:
Acta Biochimica Polonica; 2001, 48, 4; 1191-1195
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Chelating ability of proctolin tetrazole analogue
Autorzy:
Łodyga-Chruścińska, Elżbieta
Sanna, Daniele
Micera, Giovanni
Chruściński, Longin
Olejnik, Jadwiga
Nachman, Ronald
Zabrocki, Janusz
Powiązania:
https://bibliotekanauki.pl/articles/1041268.pdf
Data publikacji:
2006
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
metallopeptides
metal complexes
proctolin
tetrazole peptide analogue
Opis:
The aim of the investigation was to establish the chelating ability of a new proctolin analogue of the sequence Arg-Tyr-LeuΨ[CN4]Ala-Thr towards copper(II) ions. The insertion of the tetrazole moiety into the peptide sequence has considerably changed the coordination ability of the ligand. Potentiometric and spectroscopic (UV-Vis, CD, EPR) results indicate that the incorporation of 1,5-disubstituted tetrazole ring favours the formation of a stable complex form of CuH-1L. This 4N coordination type complex is the dominant species in the physiological pH range. The tetrazole moiety provides one of these nitrogens. The data indicate that Cu(II) ions are strongly trapped inside the peptide backbone. These findings suggest that Cu(II) can hold peptide chains in a bent conformation. This bent conformation may be essential for bioactivity of the tetrazole peptides.
Źródło:
Acta Biochimica Polonica; 2006, 53, 1; 65-72
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Gene expression and peptide localization for LH-hCG receptor in porcine small and large luteal cells: possible regulation by opioid peptides
Autorzy:
Kaminski, T.
Gawronska, B.
Derecka, K.
Okrasa, S.
Przala, J.
Powiązania:
https://bibliotekanauki.pl/articles/69878.pdf
Data publikacji:
2000
Wydawca:
Polskie Towarzystwo Fizjologiczne
Tematy:
reverse transcription
opioid peptide
gene expression
immunocytochemistry
polymerase chain reaction
peptide localization
porcine luteal cell
Źródło:
Journal of Physiology and Pharmacology; 2000, 51, 2
0867-5910
Pojawia się w:
Journal of Physiology and Pharmacology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Chicken meat proteins as potential precursors of bioactive peptides
Autorzy:
Dziuba, J.
Minkiewicz, P.
Plitnik, K.
Powiązania:
https://bibliotekanauki.pl/articles/1371527.pdf
Data publikacji:
1996
Wydawca:
Instytut Rozrodu Zwierząt i Badań Żywności Polskiej Akademii Nauk w Olsztynie
Tematy:
peptide
proteolysis
food biochemistry
tropomyosin
Gallus gallus
collagen
meat protein
chicken meat
myosin
troponin
bioactive peptide
protein
chicken
Opis:
Amino acid sequences of chicken (Gallus gallus) meat proteins: myosin, tropomyosin, troponin, collagen and connectin taken from SWISS-PROT and EMBL databases have been analysed using "PROTEIN" computer program searching for fragments identical to bioactive peptides and for bonds susceptible to the action of endopeptidases in protein chains. Chicken meat proteins contain fragments with antihypertensive (connectin), immunomodulating (myosin, tropomyosin, collagen), antithrombotic (collagen), antibacterial (collagen), embryotoxic (collagen) activity and also neuroactive (myosin, collagen, connectin) occurring in amino acid sequences with the frequency higher than that expected from the probability of appearance of given fragments in random amino acid sequences. There is a theoretical possibility of release of bioactive fragments from chicken meat proteins by endopeptidases. Such possibility especially occurs in the case of hydrolysis by proteinase K (EC 3.4.21.14). The frequency of occurrence of bioactive fragments may be applied for quantitative comparison of value of proteins as a source of bioactive peptides, although different affinity of bioactive fragments to their receptors and different susceptibility of proteins to proteolysis should be taken into consideration.
Sekwencje aminokwasowe białek mięsa kurcząt (Gallus gallus): miozyny, tropomiozyny, troponiny, kolagenu i konektyny pochodzące z baz danych SWISS-PROT i E MB L były analizowane za pomocą programu komputerowego PROTEIN wyszukującego w łańcuchach białek fragmenty identyczne z sekwencjami bioaktywnych peptydów oraz wiązania podatne na działanie endopeptydaz. Białka mięsa kurcząt zawierają fragmenty o aktywności przeciwnadciśnieniowej (konektyna) (rys. 3), immunomodulacyjnej (miozyna, tropomiozyna, kolagen), przeciwkrzepliwej (kolagen), antybakteryjnej (kolagen), embriotoksycznej (kolagen) (rys. 2) oraz neuroaktywne (miozyna, kolagen, konektyna) (rys. 2, 3) występujące w sekwencjach aminokwasowych z częstością większą, niż przewidywana na podstawie prawdopodobieństwa pojawienia się danych fragmentów w przypadkowych sekwencjach aminokwasowych (rys. 1). Istnieje teoretyczna możliwość uwalniania bioaktywnych peptydów z białek mięsa kurcząt przez endopeptydazy. Taka możliwość istnieje szczególnie w przypadku hydrolizy przez proteinazę K (EC 3.4.21.14) (rys. 2 i 3). Częstość występowania bioaktywnych fragmentów może służyć do ilościowego porównywania potencjalnej wartości białek jako źródła bioaktywnych peptydów, chociaż musi być uwzględnione także różne powinowactwo fragmentów do ich receptorów oraz różna podatność białek na proteolizę.
Źródło:
Polish Journal of Food and Nutrition Sciences; 1996, 05, 4; 85-96
1230-0322
2083-6007
Pojawia się w:
Polish Journal of Food and Nutrition Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Cathelicidin LL-37: LPS-neutralizing, pleiotropic peptide
Autorzy:
Golec, M
Powiązania:
https://bibliotekanauki.pl/articles/51492.pdf
Data publikacji:
2007
Wydawca:
Instytut Medycyny Wsi
Tematy:
human organism
human disease
asthma
lipopolysaccharide
peptide
pathogenic microorganism
organic dust
cathelicidin
antimicrobial peptide
dust
endotoxin
Opis:
Human organism, constantly exposed to a large variety of pathogenic microorganisms and their products, such as lipopolysaccharide (LPS), developed innate immunity as a fi rst line of defence. One of the compartments of our organism well equipped with these defence mechanisms is the respiratory system. The cells lining the airways respond to the presence of virulent microorganisms by producing natural antimicrobial peptides, including the only member of the cathelicidins family found to date in humans, peptide LL-37. LL-37 is a small peptide of 37 amino acid residues. The peptide, in addition to its bactericidal effect, plays numerous roles in infl ammatory and tissue remodelling processes. It stimulates angiogenesis, induces proliferation of lung epithelial cells, accelerates wound closure of the airway epithelium, and provokes cytokine release (e.g. IL-8) and cell migration. LL-37 is also able to neutralize LPS, a heteropolymer associated with organic dust, produced by Gram-negative bacteria. LPS (commonly referred to as endotoxin) plays an important role in pathogenesis of many respiratory diseases caused by organic dust, including organic dust toxic syndrome and chronic illnesses such as chronic obstructive pulmonary disease (COPD), asthma or allergic alveolitis (hypersensitivity pneumonitis). LPS is a strong pro-infl ammatory stimulus, inducing in respiratory airways expression of antimicrobial peptides, including LL-37, which is in turn a potent LPS-neutralizing factor. The article discusses the complex interplay between endotoxin and the LPS-neutralizing, pleiotropic peptide LL-37 in pathogenic mechanisms of lung diseases, with regard to closer perspectives of using LL-37 and its derivatives as therapeutic agents.
Źródło:
Annals of Agricultural and Environmental Medicine; 2007, 14, 1
1232-1966
Pojawia się w:
Annals of Agricultural and Environmental Medicine
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Detekcja enzymów z wykorzystaniem macierzy polimer-substrat
Enzyme assays based on polymer-substrate arrays
Autorzy:
Trzcińska, R.
Utrata-Wesołek, A.
Suder, P.
Dworak, A.
Silberring, J.
Trzebicka, B.
Powiązania:
https://bibliotekanauki.pl/articles/285196.pdf
Data publikacji:
2011
Wydawca:
Akademia Górniczo-Hutnicza im. Stanisława Staszica w Krakowie. Polskie Towarzystwo Biominerałów
Tematy:
macierze peptydowe
koniugaty polimer-peptyd
enzymy
powierzchnie
peptydy
peptide arrays
conjugates polymer-peptide
enzymes
surfaces
peptides
Opis:
Ocena aktywności enzymów jest kluczowym zagadnieniem w badaniu kinetyki reakcji enzymatycznych i inhibicji. Ostatnio, macierze polimerowo-peptydowe, gdzie peptyd jest specyficznym substratem dla enzymu wzbudzają coraz większe zainteresowanie wśród biochemików. W połączeniu z czułymi metodami detekcji takimi jak spektrometria mas i fluorescencja macierze mogą znaleźć zastosowanie jako niezwykle użyteczne narzędzia do detekcji enzymów i oceny ich aktywności w diagnostyce klinicznej. Macierze peptydowe składają się z powierzchni, na której immobilizowane są peptydy . Peptydy mogą być przyłączone bezpośrednio do sfunkcjonalizowanej powierzchni lub poprzez polimerowy linker. Obecność polimerowego linkera pomiędzy powierzchnią a peptydem przynosi wiele korzyści. Zastosowanie linkera z poli(tlenku etylenu) minimalizuje niespecyficzną adsorpcję biomolekuł na podłożu, zapewnia niskie tło w pomiarach fluorescencyjnych i ułatwia dostęp steryczny centrum aktywnego do peptydu. W niniejszej pracy koniugaty peptydów z poli(tlenkiem etylenu) zostały otrzymane na drodze syntezy na nośniku stałym metodą Fmoc. Otrzymane koniugaty immobilizowano na powierzchni krzemu zmodyfikowanej aminopropy lotrietoksy silanem. Powierzchnię scharakteryzowano techniką AFM i zbadano jej kąt zwilżania. Tak otrzymane macierze peptydowe poddano inkubacji z enzymami a produkty reakcji analizowano za pomocą techniki ESI MS.
Enzyme assays are methods for measuring enzymatic activity, they are vital to study enzyme kinetics and enzyme inhibitors. Recently, polymer-peptide arrays where the peptide is specific substrate for the enzyme have attracted high attention. Such arrays can be applied as invaluable tools for enzyme recognition when connected to fluorescence or mass spectrometry detection. Peptide arrays consist of peptides immobilized on solid support directly or through polymeric linker. It w as shown that the presence of poly(ethylene oxide) as a linker between surface and the peptide offers many advantages. It minimizes non-specific binding of biomolecules on the surface, provides low background in fluorescence measurement, avoids steric hindrance , and thus makes linked peptide fully accessible the active site of an enzyme. In this work, conjugate of peptide and poly(ethylene oxide) were synthesized on the Tentagel resin using solid phase Fmoc chemistry, and then covalently immobilized by grafting-to method on the silica surface. Silica plates before grafting were modified by 3-aminopropy ltriethoxy silane and characterized by AFM and contact angel measurement. The obtained peptide arrays were incubated with enzymes and products of these reactions were analyzed by ESI MS.
Źródło:
Engineering of Biomaterials; 2011, 14, no. 109-111 spec. iss.; 36
1429-7248
Pojawia się w:
Engineering of Biomaterials
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Use of o-phosphoserine [OPS] for the separation of peptides on immobilized copper ions
Wykorzystanie OPS w procesie rozdzialu peptydow na unieruchomionych jonach miedzi
Autorzy:
Karas, M
Powiązania:
https://bibliotekanauki.pl/articles/14435.pdf
Data publikacji:
2010
Wydawca:
Uniwersytet Warmińsko-Mazurski w Olsztynie / Polskie Towarzystwo Magnezologiczne im. Prof. Juliana Aleksandrowicza
Tematy:
o-phosphoserine
separation
peptide
immobilized copper ion
copper ion
metal ion
protein
diet component
bioactive peptide
Opis:
Recent research into the structure and properties of proteins and peptides as physiologically active diet components has spurred a new interest in the isolation and investigation of bioactive peptides of animal, plant and microbiological origin. The isolation and separation of protein and peptide mixtures requires advanced procedures. It usually involves a multi-stage separation process on chromatographic columns with various packing. Immo- bilised Metal Ion Affinity Chromatography (IMAC) is frequently used in the complex process of obtaining peptide fractions. Affinity Chromatography (IMAC) relies on the specific interactions between amino acids, their reactive groups in proteins and peptides and „transitory” metal ions, in particular Cu2+. Those ions are immobilised by the chelating compound on the bed, forming specific adsorbents which bind proteins and peptides. The aim of this study was to determine whether o-phosphoserine (OPS) can be used for the immobilization of copper ions on Sephadex G25 during the separation of peptides and proteins isolated from string beans. Frozen pods of dwarf, green-podded string bean cv. Fana were used in the study. Peptide were extracted from well-homogenized string bean pods with tris-HCl buffer (pH 7.5), from which high molecular weight proteins were isolated with methanol, acetone, 20% trichloroacetic acid and the Magnafloc M-22S cation flocculant. The protein and peptide content of the separated fractions was determined. The peptide content depended on the type of extract from which high molecular weight proteins were isolated. The results obtained by using OPS as a chelating agent in the separation of string bean can be recommended for analysis of plant peptides.
Rozwój nauki o strukturze oraz właściwościach białek i peptydów jako fizjologicznie aktywnych składnikach diety przyczynił się do wzrostu zainteresowania izolowaniem i badaniem bioaktywnych peptydów pochodzenia zwierzęcego, roślinnego i mikrobiologicznego. Izolowanie i rozdział mieszanin białek i peptydów wymaga zaawansowanej procedury. Stosuje się zazwyczaj kilkustopniowy rozdział na kolumnach chromatograficznych z różnym wypełnieniem. W tak skomplikowanym procesie otrzymywania frakcji peptydowych szerokie zastosowanie znalazła chromatografia powinowactwa na unieruchomionych jonach metali IMAC (Immobilized Metal Ion Affinity Chromatography). Chromatografia powinowactwa wykorzystuje specyficzne oddziaływania między aminokwasami oraz ich reaktywnymi ugrupowaniami w białkach i peptydach a jonami metali „przejściowych”, szczególnie zaś z Cu2+. Jony te są immobilizowane przez związek chelatujący na złożu, i w ten sposób stanowią specyficzne adsorbenty wiążące białka lub peptydy. Celem pracy było zbadanie przydatności OPS (o-fosfoseryny) jako czynnika unieruchamiającego jony miedzi na złożu w procesie rozdziału peptydów i białek wyizolowanych z fasoli szparagowej metodą IMAC. Materiałem do badań były mrożone strąki fasoli szparagowej karłowatej zielonostrąkowej, odmiany Fana. Peptydy i białka izolowano z fasoli szparagowej buforem Tris-HCl, z otrzymanego ekstraktu białka wysokocząsteczkowe wydzielono: metanolem, acetonem, 20% kwasem trichlorooctowym i kationowym flokulantem Magnafloc M-22S. W otrzymanych frakcjach oznaczono zawartość białka i peptydów. Peptydy obecne w fasoli szparagowej charakteryzowały się zbliżonym powinowactwem do jonów miedzi. Wykazano, że rozdział peptydów zależy w małym stopniu od właściwości czynnika zastosowanego podczas usuwania białek z ekstraktu. Przebieg rozdziału z wykorzystaniem OPS jako czynnika chelatującego w technice IMAC z powodzeniem może być stosowany do rozdziału peptydów z ekstraktów roślinnych.
Źródło:
Journal of Elementology; 2010, 15, 1; 101-110
1644-2296
Pojawia się w:
Journal of Elementology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Antinociceptive effect of MAS MT in rats
Autorzy:
Rykaczewska-Czerwinska, M.
Radosz, A.
Szymanowska-Dziubasik, K.
Konopinska, D.
Plech, A.
Powiązania:
https://bibliotekanauki.pl/articles/55342.pdf
Data publikacji:
2008
Wydawca:
Sieć Badawcza Łukasiewicz - Instytut Przemysłu Organicznego
Tematy:
Tenebrio molitor
antinociceptive effect
rat
larva
brain
neuropeptide
insect neuropeptide
Manduca sexta
proctolin
myotropin
biological activity
peptide chain
peptide
Opis:
MAS MT is a myotropic decapeptide isolated from Manduca sexta. This peptide exerts stimulatory effect on insects heart-beat frequency. The present study was undertaken in order to determine a probable antinociceptive effect in rats of native synthetic decapeptide, MAS MT-I and its two analogs, heptapeptides MAS MT-II and MAS MT-III. All these peptides were applied directly into the lateral brain ventricle (icv) at three doses: 10, 25 and 50 nmol. The analgesic (antinociceptive) effect was evaluated by a tail immersion test. It was found that two doses of MAS MT-I: 25 and 50 nmol induced significant antinociceptive effect, while MAS MT-II and MAS MT-III exert a less antinociceptive effect in comparison with native MAS MT-I. Prior icv administration of naloxone, an opioid antagonist weakly blocked MAS MT-I effect. We conclude that antinociceptive effect of MAS MT-I in rats is not mediated by central opioid system.
Źródło:
Pestycydy; 2008, 3-4; 139-146
0208-8703
Pojawia się w:
Pestycydy
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Synthesis and application of chiral triazine condensing reagents prepared from esters of amino acids.
Autorzy:
Kamiński, Zbigniew
Zając, Krzysztof
Jastrząbek, Konrad
Powiązania:
https://bibliotekanauki.pl/articles/1044064.pdf
Data publikacji:
2001
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
enantioselective reagent
coupling reagent
chiral triazines
peptide synthesis
Opis:
Treatment of cyanuric chloride with chiral amines or esters of chiral amino acids gave chiral 2,4-dichloro-6-alkylamino-1,3,5-triazines (2-5) in 49-69% yield, which were found useful as coupling reagents. Enantioselective activation and enantioselective aminolysis in the presence of 2-5 was observed.
Źródło:
Acta Biochimica Polonica; 2001, 48, 4; 1143-1146
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Supported liquid membrane extraction of peptides.
Autorzy:
Drapała, Anna
Dżygiel, Paweł
Jönsson, Jan
Wieczorek, Piotr
Powiązania:
https://bibliotekanauki.pl/articles/1044056.pdf
Data publikacji:
2001
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
Aliquat 336
peptide
extraction
supported liquid membrane
Opis:
The application of supported liquid membrane (SLM) extraction for the enrichment of short peptides is presented. The extraction efficiency is dependent on the pH of donor phase and salt concentration in acceptor phase. Moreover, the extraction efficiency is also influenced by the peptide amino-acid sequence and hydrophobicity.
Źródło:
Acta Biochimica Polonica; 2001, 48, 4; 1113-1116
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Eliminating side effects of pain therapies
Autorzy:
Różycki, Krzysztof
Rapacz, Jacek
Fichna, Jakub
Kosson, Piotr
Powiązania:
https://bibliotekanauki.pl/articles/1177402.pdf
Data publikacji:
2018
Wydawca:
Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
Tematy:
PAMORA
cancer
constipation
morphine
opioid
pain
peptide
Opis:
Pain is a very serious problem associated with many types of health conditions. Unfortunately, patients suffering from intense pain often give up or cut down on taking analgesics because of side effects of such treatment. New generation drugs create an opportunity for supporting therapies effectively. Persistent constipation is one of the more acute side effects of administration of opioids. This article gives an extensive coverage of the PAMORA group of drugs that eliminate opioid-induced constipation. It also proposes future directions for contemporary studies on the subject and other feasible therapeutic applications for this group of medicines. The molecule developed under the POIG.01.04.00-14-213/12 project “New intestinal drug for control of side effects of opioid therapies” is one of the prospects. [The project] researched structures based on opioid peptide analogs behaving like opioid antagonists vs. intestinal μ receptors. In addition, [the structures] feature properties that can be used in oncological therapies because they inhibit growth of malignant cells. The structures are being tested on animal models.
Źródło:
World Scientific News; 2018, 104; 400-410
2392-2192
Pojawia się w:
World Scientific News
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Who stole the sugar? Recurrent hypoglycemia in three women
Autorzy:
Gontarz, Katarzyna
Barczykowska, Anna
Głowacka, Paulina
Mędza, Aleksandra
Młynarkiewicz, Małgorzata
Obrębski, Jakub
Szczurek, Małgorzata
Wielewicka, Aleksandra
Obołończyk, Łukasz
Wiśniewski, Piotr
Powiązania:
https://bibliotekanauki.pl/articles/895783.pdf
Data publikacji:
2018-09-28
Wydawca:
Gdański Uniwersytet Medyczny
Tematy:
factitious disorder
Munchausen syndrome
hyperinsulinemia
C-peptide
Opis:
This article presents 3 cases that highlight one of the factitious disorders named Munchausen Syndrome (MS). It is defined as intentional simulation or self-induction of disease symptoms to gain attention of others and to be perceived as an ill person. Early recognition of factitious disorders is a challenge for non-psychiatrists, as its clinical symptoms vary significantly among patients. In this paper we present three women with recurrent episodes of hypoglycemia, who were eventually diagnosed with MS. Our aims were to share the diagnostic clues that can suggest the presence of a factitious disorder, to highlight the analysis of patient’s medical history and to suggest the potential the ethical dilemmas involved in caring for such patients.
Źródło:
European Journal of Translational and Clinical Medicine; 2018, 1, 1; 70-75
2657-3148
2657-3156
Pojawia się w:
European Journal of Translational and Clinical Medicine
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Novel peptide recognized by RhoA GTPase
Autorzy:
Drulis-Fajdasz, Dominika
Jelen, Filip
Oleksy, Arkadiusz
Otlewski, Jacek
Powiązania:
https://bibliotekanauki.pl/articles/1041207.pdf
Data publikacji:
2006
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
GrafGAP
RhoA GTPase
epitope mapping
PDZRhoGEF
peptide phage display
Opis:
A phage-displayed random 7-mer disulfide bridge-constrained peptide library was used to map the surface of the RhoA GTPase and to find peptides able to recognize RhoA switch regions. Several peptide sequences were selected after four rounds of enrichment, giving a high signal in ELISA against RhoA-GDP. A detailed analysis of one such selected peptide, called R2 (CWSFPGYAC), is reported. The RhoA - R2 interaction was investigated using fluorescence spectroscopy, chemical denaturation, and determination of the kinetics of nucleotide exchange and GTP hydrolysis in the presence of RhoA regulatory proteins. All measurements indicate that the affinity of the R2 peptide for RhoA is in the micromolar range and that R2 behaves as an inhibitor of: i) GDP binding to the apo form of RhoA (Mg2+- and nucleotide-free form of the GTPase), ii) nucleotide exchange stimulated by GEF (DH/PH tandem from PDZRhoGEF), and iii) GTP hydrolysis stimulated by the BH domain of GrafGAP protein.
Źródło:
Acta Biochimica Polonica; 2006, 53, 3; 515-524
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Discrete dynamic system oriented on the formation of prebiotic dipeptides from Rodes experiment
Autorzy:
Polanco, Carlos
Samaniego, José
Buhse, Thomas
Castañón González, Jorge
Powiązania:
https://bibliotekanauki.pl/articles/1039201.pdf
Data publikacji:
2014
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
origins of life
biogenesis
dipeptides
salt-induced peptide formation
Opis:
This work attempts to rationalize the possible prebiotic profile of the first dipeptides of about 4 billion years ago based on a computational discrete dynamic system that uses the final yields of the dipeptides obtained in Rode's experiments of salt-induced peptide formation (Rode et al., 1999, Peptides 20: 773-786). The system built a prebiotic scenario that allowed us to observe that (i) the primordial peptide generation was strongly affected by the abundances of the amino acid monomers, (ii) small variations in the concentration of the monomers have almost no effect on the final distribution pattern of the dipeptides and (iii) the most plausible chemical reaction of prebiotic peptide bond formation can be linked to Rode's hypothesis of a salt-induced scenario. The results of our computational simulations were related to former simulations of the Miller, and Fox & Harada experiments on amino acid monomer and oligomer generation, respectively, offering additional information to our approach.
Źródło:
Acta Biochimica Polonica; 2014, 61, 4; 717-726
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Free energy of helix propagation in short polyalanine chains determined from peptide growth simulations of La3+-binding model peptides. Comparison with experimental data
Autorzy:
Maciejczyk, Maciej
Hermans, Jan
Bierzyński, Andrzej
Powiązania:
https://bibliotekanauki.pl/articles/1041277.pdf
Data publikacji:
2006
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
thermodynamics
peptide
helix-coil equilibrium
thermodynamic integration
molecular dynamics
Opis:
Molecular dynamics (MD) is, at present, a unique tool making it possible to study, at the atomic level, conformational transitions in peptides and proteins. Nevertheless, because MD calculations are always based on a more or less approximate physical model, using a set of approximate parameters, their reliability must be tested by comparison with experimental data. Unfortunately, it is very difficult to find a peptide system in which conformational transitions can be studied both experimentally and using MD simulations so that a direct comparison of the results obtained in both ways could be made. Such a system, containing a rigid α-helix nucleus stabilized by La3+ coordination to a 12-residue sequence taken from an EF-hand protein has recently been used to determine experimentally the helix propagation parameters in very short polyalanine segments (Goch et al. (2003) Biochemistry 42: 6840-6847). The same parameters were calculated here for the same peptide system using the peptide growth simulation method with, alternatively, charmm 22 and cedar potential energy functions. The calculated free energies of the helix-coil transition are about two times too large for cedar and even three times too large for charmm 22, as compared with the experimental values. We suggest that these discrepancies have their origin in the incorrect representation of unfolded peptide backbone in solution by the molecular mechanics force fields.
Źródło:
Acta Biochimica Polonica; 2006, 53, 1; 121-130
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Conformational analysis of fragment of human Pin1 ww domain: influence of charged amino-acid residues on β-hairpin structure
Autorzy:
Makowska, J.
Uber, D.
Żmudzińska, W.
Chmurzyński, L.
Powiązania:
https://bibliotekanauki.pl/articles/1935818.pdf
Data publikacji:
2014
Wydawca:
Politechnika Gdańska
Tematy:
peptide conformation
β-hairpin
hPin1 protein
NMR
Opis:
We examined the effect of like-charged residues on the conformation of an original nine amino-acid-residue fragment of the human Pin1 WW domain (hPin1) with the following sequence: Ac-Arg-Met-Ser-Arg-Ser-Ser-Gly-Arg-Val-NH 2 (U9). This was facilitated by CD and NMR spectroscopic measurements, and molecular dynamics calculations. Our ear lier studies suggested that the presence of like-charged residues at the end of a short polypeptide chain composed of nonpolar residues could induce a chain reversal. For the U9 peptide, canonical MD simulations with NMR -derived restraints demonstrated the presence of ensembles of structures with a tendency to form a β -chain reversal. Additionally, thermal stabilities of the peptide under study were measured using differential scanning calorimetry ( DSC ). The estimated well defined phase transition point showed that conformational equilibria in the U9 peptide were strongly dependent on temperature.
Źródło:
TASK Quarterly. Scientific Bulletin of Academic Computer Centre in Gdansk; 2014, 18, 4; 343--349
1428-6394
Pojawia się w:
TASK Quarterly. Scientific Bulletin of Academic Computer Centre in Gdansk
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Solution structure of conformationally restricted vasopressin analogues.
Autorzy:
Trzepałka, Emilia
Oleszczuk, Marta
Maciejczyk, Maciej
Lammek, Bernard
Powiązania:
https://bibliotekanauki.pl/articles/1043316.pdf
Data publikacji:
2004
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
vasopressin
X-PLOR
NMR spectroscopy
EDMC
peptide conformation
Opis:
In recent years, a massive effort has been directed towards designing potent and selective antagonists of neurohypophyseal hormones substituted at position 3. Modification of vasopressin at position 3 with 4,4'-biphenylalanine results in pharmacologically inactive analogues. Chemically, this substitution appears to vary only slightly from those previously made by us (1-Nal or 2-Nal), which afforded potent agonists of V2 receptors. In this situation, it seemed worthwhile to study the structure of the analogues with 4,4'-biphenylalanine (BPhe) at position 3 in aqueous solution using NMR spectroscopy and total conformational analysis. This contribution is part of extensive studies aimed at understanding spatial structures of 3-substituted [Arg8]vasopressin analogues of different pharmacological properties. NMR data were used to calculate 3D structures for all the analogues using two methods, EDMC with the ECEPP/3 force field, and molecular dynamic with the simulated annealing (SA) algorithm. The structures obtained by the first method show a better fit between the NMR spectral evidence and the calculation for all the peptides.
Źródło:
Acta Biochimica Polonica; 2004, 51, 1; 33-49
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Synthetic extracellular matrix as a substrate for regenerative medicine
Autorzy:
Stodolak-Zych, Ewa
Menaszek, Elżbieta
Twardy, Aleksandra
Ścisłowska-Czarnecka, Anna
Boguń, Maciej
Kolesińska, Beata
Powiązania:
https://bibliotekanauki.pl/articles/285740.pdf
Data publikacji:
2019
Wydawca:
Akademia Górniczo-Hutnicza im. Stanisława Staszica w Krakowie. Polskie Towarzystwo Biominerałów
Tematy:
synthetic extracellular matrix
biomimetic
peptide
polysaccharides
laboratory model
Opis:
The work presents materials characteristics of fibrous polysaccharide substrates (calcium alginate, CA) modified with short peptides. Three types of synthesized peptides (hexapeptides) were composed of: cysteine (C) and tryptophan (W) named - (WWC)2or cysteine (C) and tyrosine (Y) named (YYC)2 or phenyloalanine (F) named 6F. The peptides size distribution (DLS method) showed that they agglomerated in an alcohol medium. These results were used to select a modification method of the fibrous substrates i.e. the peptides were deposited on the fibrous alginate substrate by the electrospraying technique. Using this method three kinds of polysaccharide- peptides systems were obtained i.e.: CA/(WWC)2, CA/(YYC)2CA/6F. As a reference material, the pure calcium alginate fibrous substrate was used. The results of modification with short peptides were evaluated via scanning electron microscopy (SEM): small aggregates were observed (40-100 nm) on the surface of fibers, and the fibers size remained the same after modification (11-12 μm). The size of aggregates depended on the kind of short peptide; the smaller (40 nm) aggregates were observed when the peptide had only aromatic chain (6F), the bigger (<100 nm) ones were observed when the peptide had heterocyclic rings in the chain (WWC and YYC). All materials were contacted with osteoblast-like cells (MG-63) to test biocompatibility (cells viability after 3 and 7 days) and the results proved showed higher viability in the polysaccharide-peptide system which increased with the time of observation. The durability of polysaccharide-peptide systems was tested using the enzymatic assay: collagenase confirmed the stability of materials. The progress of degradation rate was observed using infrared spectroscopy (FTIR-ATR) - the ratio on bands with C-O and C-OH increased after degradation under in vitro conditions.Results of the investigations on the fibrous substrates have confirmed that the system is a good model of an extracellular matrix (ECM) due to its chemical composition and microstructure which both have biomimetic characteristics. Thus, it may be used as a filling of bone defects supporting the regeneration of the damaged tissue. Additionally, it may also serve as the model research system of ECM.
Źródło:
Engineering of Biomaterials; 2019, 22, 152; 10-15
1429-7248
Pojawia się w:
Engineering of Biomaterials
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
DIAGNOSTIC POTENTIAL OF SELECTED SALIVARY PROTEOMICS FOR AUTONOMIC NERVOUS SYSTEM ACTIVITY ASSESSMENT
Autorzy:
Dobrek, Lukasz
Powiązania:
https://bibliotekanauki.pl/articles/2137800.pdf
Data publikacji:
2020-05-14
Wydawca:
Fundacja Edukacji Medycznej, Promocji Zdrowia, Sztuki i Kultury Ars Medica
Tematy:
saliva
vasoactive intestinal peptide
neuropeptide Y
chromogranin A
α-amylase
Opis:
The clinical assessment of autonomic nervous system (ANS) functioning, enabling the diagnosis of autonomic neuropathy present in the course of many diseases, is currently based on performing simple cardiovascular reflexes (Ewing tests), analyzing heart rate variability (HRV) or heart rate turbulence (HRT), examining skin sweating or recording neurophysiological tests (e.g. microneurography). Laboratory assessment of ANS function is very scarce and practically only includes the plasma assessment of noradrenaline as a surrogate for the biochemical indicator of sympathetic activity. Recently, the possibility of evaluation of selected compounds present in saliva as laboratory markers of not only oral diseases but also systemic diseases has been raised. This work focuses on a brief description of the anatomy and physiology of the salivary glands and describes the formation of saliva, its composition and the use of this bodily fluid in laboratory diagnostics. In addition, the paper specifically discusses the possibility of determining selected compounds that are considered to reflect autonomic activity. A review of the literature indicates primarily four proteomics: two neuropeptides (vasoactive intestinal peptide (VIP) and neuropeptide Y (NPY) that are co-transmitters in autonomic fibers, chromogranin A, a synaptic vesicle protein and α-amylase, a hydrolytic enzyme pre-digesting carbohydrates in the oral cavity. These are currently the most widely investigated agents for their usefulness as laboratory markers of ANS activity.
Źródło:
Acta Neuropsychologica; 2020, 18(2); 285-303
1730-7503
2084-4298
Pojawia się w:
Acta Neuropsychologica
Dostawca treści:
Biblioteka Nauki
Artykuł
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