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Wyszukujesz frazę "electrophoresis" wg kryterium: Temat


Tytuł:
Bioactive coatings obtained by electrophoretic deposition on Ti15Mo alloy
Autorzy:
Szklarska, M.
Dercz, G.
Łosiewicz, B.
Stróż, D.
Powiązania:
https://bibliotekanauki.pl/articles/283998.pdf
Data publikacji:
2016
Wydawca:
Akademia Górniczo-Hutnicza im. Stanisława Staszica w Krakowie. Polskie Towarzystwo Biominerałów
Tematy:
biomaterials
electrophoresis
implants
Źródło:
Engineering of Biomaterials; 2016, 19, 138; 35
1429-7248
Pojawia się w:
Engineering of Biomaterials
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Application of electrophoretic methods for detection of protein-porphyrin complexes.
Autorzy:
Kowalska, Agnieszka
Kwiek, Piotr
Miłosz, Ewa
Gondek, Grzegorz
Romiszewska, Anna
Graczyk, Alfreda
Podhajska, Anna
Powiązania:
https://bibliotekanauki.pl/articles/1043407.pdf
Data publikacji:
2003
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
porphyrins
photodynamic therapy
PDT
electrophoresis
Opis:
Simple methods for detection and isolation of protein-porphyrin complexes were elaborated in our laboratory. They are based on the separation of protein-porphyrin complexes in native polyacrylamide gel and measurement of their fluorescence, with the use of two detection systems: the commercially available Gel DocTM 2000 system, and a system specially designed for the purpose of these investigations, concerning protein-porphyrin interactions. The fluorescent complexes can be electro-transferred from the gel onto PVDF membrane, eluted and analyzed in order to identify the protein interacting with porphyrins.
Źródło:
Acta Biochimica Polonica; 2003, 50, 4; 1155-1163
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Separation of low molecular rapeseed proteins by capillary electrophoresis
Autorzy:
Amarowicz, Ryszard
Panasiuk, Robert
Pari, Leelavinothan
Powiązania:
https://bibliotekanauki.pl/articles/2051054.pdf
Data publikacji:
2003
Wydawca:
Instytut Rozrodu Zwierząt i Badań Żywności Polskiej Akademii Nauk w Olsztynie
Tematy:
capillary electrophoresis
rapeseed
low molecular proteins
Opis:
Low molecular proteins were extracted and isolated from rapeseed. The main protein were characterised with molecular mass of 14 000 determined using capillary electrophoresis-SDS technique. UV spectrum showed that this protein appears as a complex with phenolic acids.
Źródło:
Polish Journal of Food and Nutrition Sciences; 2003, 53, 1s; 7-9
1230-0322
2083-6007
Pojawia się w:
Polish Journal of Food and Nutrition Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Genetic diversity of winter wheat cultivars and strains determined by electrophoregrams of gliadin and glutenin proteins
Autorzy:
Węgrzyn, Stanisław
Waga, Jacek
Powiązania:
https://bibliotekanauki.pl/articles/2198933.pdf
Data publikacji:
2004-06-20
Wydawca:
Instytut Hodowli i Aklimatyzacji Roślin
Tematy:
cluster analysis
electrophoresis
gliadins
glutenins
polymorphism
Opis:
Based on the polymorphism of gliadin and glutenin proteins relationships of 45 cultivars and strains of winter wheat were evaluated. The cluster analysis showed a considerable variation of the investigated genotypes. The similarity indices were calculated using the Nei and Li formula. The genetic distances between the cultivars ranged from 1.00 to 0.12. The highest similarity index - SI=1.00- being proof of the identical physicochemical composition of storage proteins, was found for the pair Farmer and Elena. The groups of similar and genetically distant cultivars have been presented in the form of a dendrogram. The possibility of using the results obtained from the cluster analysis in breeding programmes has been discussed.
Źródło:
Plant Breeding and Seed Science; 2004, 49; 51-61
1429-3862
2083-599X
Pojawia się w:
Plant Breeding and Seed Science
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
B-cell chronic lymphocytic leukemia-associated nuclear antigens
Autorzy:
Rogalińska, Małgorzata
Błoński, Jerzy Z
Robak, Tadeusz
Kiliańska, Zofia M
Powiązania:
https://bibliotekanauki.pl/articles/764933.pdf
Data publikacji:
2008
Wydawca:
Uniwersytet Łódzki. Wydawnictwo Uniwersytetu Łódzkiego
Tematy:
B-CLL specific antigens
electrophoresis
immunoblotting
Opis:
One- and two-dimensional polyacrylamide gel electrophoresis were used to compare the composition of nuclear polypeptides from normal and В-cell chronic lymphocytic leukemia mononuclear cells. Against two electrophoretically-specific nuclear proteins with molecular weight of 38/39 and 44/46 kD a from leukemic cells rabbit sera were obtained. As it was analyzed by Western blot technique the available antisera recognized the 38/39 kDa antigen in 53 of the 56 (94.6%), while the 44/46 kDa in 46 of the 49 (93.9%) of examined В-CLL nuclear fraction preparations, but not in normal ones. The pi values of described leukaemia-specific antigens were determined; p38/39 had pi in the range of pH 6.55 -7.00 and p44/46 - in the range of pH 6.2-6.4.
Źródło:
Acta Universitatis Lodziensis. Folia Biologica et Oecologica; 2008, 4
1730-2366
2083-8484
Pojawia się w:
Acta Universitatis Lodziensis. Folia Biologica et Oecologica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Poziom frakcji białkowych surowicy krwi u nurków
Levels of protein fractions in diver’s blood serum
Autorzy:
Dolatkowski, A.
Szczeblewski, B.
Lokucijewski, B.
Kudarenko, W.
Powiązania:
https://bibliotekanauki.pl/articles/1360608.pdf
Data publikacji:
2014
Wydawca:
Polskie Towarzystwo Medycyny i Techniki Hiperbarycznej
Tematy:
nurek
nurkowanie
białka krwi
elektroforeza białek
elektroforeza bibułowa
diver
diving
blood proteins
protein electrophoresis
paper electrophoresis
Opis:
Badano osocze krwi 72 nurków w wieku 21 do 23 lat nurków metodą elektroforezy bibułowej. Wartości frakcji białkowych w badaniu wyniosły: albuminy 61,7%, α1 globuliny 26%, α2 globuliny 6%, β globuliny 9,5% γ globuliny 20,2%. Wartości te mieściły się w normie fizjologicznej, ale największy procent odchyleń obserwowano w grupie I – nurków początkujących. Autorzy wiążą tę obserwację z możliwością działania czynnika emocjonalnego i chwilowymi zaburzeniami regulacji hormonalnej.
The study consisted in an analysis of the blood plasma of 72 divers aged between 21 and 23 years with paper electrophoresis method. The examined protein fractions reached the following values: albumins 61.7%, α1 globulins 26%, α2 globulins 6%, β globulins 9.5% γ globulins 20.2%. The obtained values were within physiological norm, with the most significant deviations observed in group I – beginner divers. The authors link this observation with a possible impact of an emotional factor and momentary disorders in hormonal regulation.
Źródło:
Polish Hyperbaric Research; 2014, 1(46); 93-106
1734-7009
2084-0535
Pojawia się w:
Polish Hyperbaric Research
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Experimental study on electrokinetic of kaolinite particles in aqueous suspensions
Autorzy:
Min, F.
Zhao, Q.
Liu, L.
Powiązania:
https://bibliotekanauki.pl/articles/110918.pdf
Data publikacji:
2013
Wydawca:
Politechnika Wrocławska. Oficyna Wydawnicza Politechniki Wrocławskiej
Tematy:
kaolinite
zeta potential
pH
immersion time
electrophoresis
Opis:
Influence of aqueous phase properties and process parameters on kaolinite particle zeta potential was quantified by electrophoresis experiments. The results indicated that pH strongly altered the zeta potential of kaolinite and it decreases at the beginning and then increases in the range of pH = 2–13. The activity of different cations changes the zeta potential and has the following tendency of Al3+ > Ca2+ > Mg2+ > Na+ and the zeta potential increases due to heterocoagulation of different mineral particles in suspension. It was found that the zeta potential of kaolinite particles increases after the suspension was stirred and decreases at the beginning, and then increases with soaking time. The FTIR results showed that the zeta potential takes into account ion adsorption and the change of Si–O, Al–O and Al–OH groups on the surface of the kaolinite particles.
Źródło:
Physicochemical Problems of Mineral Processing; 2013, 49, 2; 659-672
1643-1049
2084-4735
Pojawia się w:
Physicochemical Problems of Mineral Processing
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Native nucleic acid electrophoresis as an efficient alternative for genotyping method of influenza virus
Autorzy:
Pajak, Beata
Lepek, Krzysztof
Powiązania:
https://bibliotekanauki.pl/articles/1039250.pdf
Data publikacji:
2014
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
gel electrophoresis
SSCP
influenza
minor genetic variants
Opis:
Influenza viruses are the worldwide major causative agents of human and animal acute respiratory infections. Some of the influenza subtypes have caused epidemics and pandemics among humans. The varieties of methods are available for the rapid isolation and identification of influenza viruses in clinical and environmental samples. Since nucleic acids amplification techniques such as RT-PCR have been adapted, fast and sensitive influenza type and subtype determination is possible. However, in some ambiguous cases other, more detailed assay might be desired. The genetic material of influenza virus is highly unstable and constantly mutates. It is known that single nucleotide polymorphisms (SNPs) results in resistance to commercially available anti-viral drugs. The genetic drift of the virus could also result in weakening of immune response to infection. Finally, in a substantial number of patients co-infection with various virus strains or types has been confirmed. Although the detection of co-infection or presence of minor genetic variants within flu-infected patients is not a routine procedure, a rapid and wide spectrum diagnostics of influenza virus infections could reveal an accurate picture of the disease and more importantly, is crucial for choosing the appropriate therapeutics and virus monitoring. Herein we present the evidences that native gel electrophoresis and MSSCP - a method based on multitemperature single strand conformation polymorphism could furnish a useful technique for minor variants, which escape discovery by conventional diagnostic assays.
Źródło:
Acta Biochimica Polonica; 2014, 61, 3; 479-483
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Determination of glyphosate in water samples with the combination of cation-exchange chromatography and capillary electrophoresis
Autorzy:
Khrolenko, M.
Dżygiel, P.
Wieczorek, P.
Powiązania:
https://bibliotekanauki.pl/articles/347017.pdf
Data publikacji:
2003
Wydawca:
Politechnika Bydgoska im. Jana i Jędrzeja Śniadeckich. Wydział Technologii i Inżynierii Chemicznej
Tematy:
glyphosate
ion-exchange chromatography
capillary electrophoresis
pesticides
Opis:
An analytical method for determination of pesticide Glyphosate in water as a combination of cation-exchange chromatography and capillary electrophoresis is presented. Pure water was spiked with Glyphosate at concentrations 0.1, 0.25, 0.5 and 1 mM and percolated through a strong cation-exchange column packed with Dowex 50WX4-400 resin in its H+ form. The extract was further analyzed by capillary electrophoresis in indirect detection mode. The calibration curve for the pesticide in the range 0.1–2.5 mM was linear and with high degree of reproducibility. The obtained recoveries for all the studied concentrations amount 85%. Afterwards, the possibility to determine Glyphosate at the concentration 0.001mM (0.17 µg/ml) was checked by percolation of 100 ml of water sample through a column. The calculated recovery was 97.7%
Źródło:
Ars Separatoria Acta; 2003, 2; 56-63
1731-6340
Pojawia się w:
Ars Separatoria Acta
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Electrophoretic seed albumin patterns in the Vicia sativa L. aggregate
Autorzy:
Przybylska, J
Zimniak-Przybylska, Z.
Krajewski, P.
Powiązania:
https://bibliotekanauki.pl/articles/2043428.pdf
Data publikacji:
2000
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
polyacrylamide gel electrophoresis
genetic resource
chromosome number
Vicia sativa
variation
DNA polymorphism
vetch
seed albumin
electrophoresis
karyotype
isoenzyme variation
taxonomy
Źródło:
Journal of Applied Genetics; 2000, 41, 3; 139-149
1234-1983
Pojawia się w:
Journal of Applied Genetics
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Azotan MDMA (3,4-metylenodioksymetamfetaminy) – nietypowa postać soli czy nowy trend na rynku narkotyków?
MDMA (3,4-methylenedioxymethamphetamine) nitrate – an atypical salt form or a new trend in the drug market?
Autorzy:
Bachliński, Robert
Galarda, Małgorzata
Powiązania:
https://bibliotekanauki.pl/articles/499846.pdf
Data publikacji:
2017
Wydawca:
Centralne Laboratorium Kryminalistyczne Policji
Tematy:
MDMA
azotan
elektroforeza kapilarna
SEM
nitrate
capillary electrophoresis
Opis:
W niniejszym artykule przedstawiony został przypadek wystąpienia na nielegalnym rynku narkotykowym nietypowej postaci 3,4-metylenodioksymetamfetaminy (MDMA) w formie azotanu. Związek taki bardzo dobrze można zidentyfikować jedynie poprzez zastosowanie metod analitycznych, których nie wykorzystuje się rutynowo w badaniach kryminalistycznych tego typu substancji – elektroforezy kapilarnej (CE) czy skaningowej mikroskopii elektronowej (SEM). Należy więc być bardzo czujnym w przypadkach, gdy badania metodą chromatografii gazowej sprzężonej ze spektrometrią masową (GC-MS) jednoznacznie identyfikują badaną substancję, ale już badania w spektrofotometrii w podczerwieni tego nie potwierdzają.
The article presents a case involving an appearance of an atypical 3,4-methylenedioxymethamphetamine (MDMA) in the form of nitrate on the illicit drug market. This compound can be identified only by using such analytical methods as capillary electrophoresis (CE) or scanning electron microscopy (SEM), which are not routinely applied to forensic analyses of this type of substances. Therefore, particular caution should be exercised whenever a gas chromatography-mass spectrometry (GC-MS) method unambiguously identifies a substance, yet infrared spectroscopy fails to confirm this result.
Źródło:
Problemy Kryminalistyki; 2017, 296; 37-45
0552-2153
Pojawia się w:
Problemy Kryminalistyki
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Determination of lanthanum(III) and europium(III) – lactate interaction constants by capillary electrophoresis for environmental applications
Autorzy:
Bortolus, S.
Varenne, A.
Draye, M.
Gareil, P.
Czerwiński, K.
Cote, G.
Powiązania:
https://bibliotekanauki.pl/articles/347001.pdf
Data publikacji:
2003
Wydawca:
Politechnika Bydgoska im. Jana i Jędrzeja Śniadeckich. Wydział Technologii i Inżynierii Chemicznej
Tematy:
lanthanides(III)
lactate
separation
interaction constants
capillary electrophoresis
Opis:
In the present work, we investigated the ability of electrophoresis to determine the stability constant of the complex formed between lactate ions (a simple model of natural organic matter), and lanthanum (III) and europium (III), two model metal cations of actinides. The effect of lactate on lanthanide’s effective mobility is studied, and conditional metal ion-ligand interaction constants are determined on the basis of the variation in the effective mobilities (log βn = 2.23 for lanthanum (III) and 2.39 for europium (III), at 25°C and I=0.015 M). The originality of this contribution lies in the fact that we have chosen to detect the metal ion instead of the ligand by an indirect UV-visible detection and using a chromophoric compound (creatinine).
Źródło:
Ars Separatoria Acta; 2003, 2; 29-35
1731-6340
Pojawia się w:
Ars Separatoria Acta
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Genetic characterization of maize inbred lines and their mutual affinities.
Autorzy:
Bulińska-Radomska, Z.
Adamczyk, J.
Królikowski, Z.
Powiązania:
https://bibliotekanauki.pl/articles/2198825.pdf
Data publikacji:
2001-06-21
Wydawca:
Instytut Hodowli i Aklimatyzacji Roślin
Tematy:
Inbred lines
enzymes
electrophoresis
similarity
pedigree
heterosis
maize
Opis:
Twenty-five inbred lines of maize assigned to six heterotic groups and developed in Poland were studied electrophoretically to determine their purity and mutual relationships. The electrophoretic data were analyzed by principal component and cluster analyses. All lines (except five) were monomorphic in each enzyme studied. Electrophoretic affinities of inbred lines were, with some exception, were in accordance with the expectation inferred from their pedigrees. Lines of identical pedigree but with small differences in phenotypic or developmental characteristics had the lowest dissimilarity coefficient values and in cluster and principal component analyses behaved accordingly. They showed however diverse levels of clustering and dissimilarity coefficient values, which most likely reflected their departure from the common parental gene pool, which occurred in the course of breeding manipulations. Lines, which had one parent in common, were less similar to each other than those with identical pedigree. The most distinct electrophretically were lines with complex or dissimilar pedigree formulae as was confirmed by cluster and principal component analyses.
Źródło:
Plant Breeding and Seed Science; 2001, 45, 1; 11-19
1429-3862
2083-599X
Pojawia się w:
Plant Breeding and Seed Science
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Development and validation of a capillary electrophoresis method for determination of oxcarbazepine and zonisamide in pharmaceuticals
Autorzy:
Paw, B.
Wos, K.
Powiązania:
https://bibliotekanauki.pl/articles/3443.pdf
Data publikacji:
2008
Wydawca:
Instytut Medycyny Wsi
Tematy:
method development
validation
capillary electrophoresis
determination
oxcarbazepine
zonisamide
drug
Źródło:
Journal of Pre-Clinical and Clinical Research; 2008, 02, 2
1898-2395
Pojawia się w:
Journal of Pre-Clinical and Clinical Research
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Single-walled carbon nanotubes fractionation via electrophoresis
Autorzy:
Scheibe, B.
Lukaszczuk, P.
Rümmeli, M. H.
Kalenczuk, R. J.
Borowiak-Palen, E.
Powiązania:
https://bibliotekanauki.pl/articles/778838.pdf
Data publikacji:
2011
Wydawca:
Zachodniopomorski Uniwersytet Technologiczny w Szczecinie. Wydawnictwo Uczelniane ZUT w Szczecinie
Tematy:
SWCNTs
sole diazoniowe
frakcjonowanie
elektroforeza
diazonium salts
fractionation
electrophoresis
Opis:
This work presents the influence of the sonication time on the efficiency of the metallic/semiconducting (M/S) fractionation of diazonium salt functionalized single-walled carbon nanotubes (SWCNTs) via free solution electrophoresis (FSE) method. The SWCNTs synthesized via laser ablation were purified from amorphous carbon and catalyst particles through high vacuum annealing and subsequent refluxing processes in aqua regia solutions, respectively. The purified material was divided into two batches. The SWCNTs samples were dispersed in 1% SDS solution in ultrasound bath for 2 and 12 hours. Both dispersed SWCNTs samples were functionalized with p-aminobenzoic acid diazonium salt and fractionated via free solution electrophoresis method. Afterwards, the fractionated samples were recovered, purified from surfactant/functionalities by annealing and investigated via UV-Vis-NIR optical absorption spectroscopy (OAS). The efficiency of the fractionation process was estimated through the comparison of the van Hove singularities (vHS) presented in the obtained fractions to the starting SWCNTs.
Źródło:
Polish Journal of Chemical Technology; 2011, 13, 3; 1-4
1509-8117
1899-4741
Pojawia się w:
Polish Journal of Chemical Technology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Peripartum changes in serum activities of three major alkaline phosphatase isoenzymes in Holstein dairy cows
Autorzy:
Chiba, A.
Onomi, R.
Hatate, K.
Moriyama, T.
Goto, A.
Yamagishi, N.
Powiązania:
https://bibliotekanauki.pl/articles/2087301.pdf
Data publikacji:
2020
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
agarose gel electrophoresis (AGE)
alkaline phosphatase isoenzyme
periparturient cow
Źródło:
Polish Journal of Veterinary Sciences; 2020, 23, 3; 457-459
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Agarose gel electrophoresis pattern of serum alkaline phosphatase isoenzymes in Holstein cows during lactation
Autorzy:
Chiba, A.
Hatate, K.
Onomi, R.
Moriyama, T.
Goto, A.
Yamagishi, N.
Powiązania:
https://bibliotekanauki.pl/articles/2087309.pdf
Data publikacji:
2020
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
agarose gel electrophoresis (AGE)
alkaline phosphatase isoenzyme
lactating cow
Źródło:
Polish Journal of Veterinary Sciences; 2020, 23, 2; 317-319
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Chromatoelektroforeza aminokwasów
Khromatoehlektroforez aminokislot
Electrophoretic separation of amino-acids
Autorzy:
Podeszewski, Z.
Powiązania:
https://bibliotekanauki.pl/articles/874411.pdf
Data publikacji:
1962
Wydawca:
Narodowy Instytut Zdrowia Publicznego. Państwowy Zakład Higieny
Tematy:
elektroforeza
chromatografia
elektroforeza dwukierunkowa
elektrochromatografia
rozklad aminokwasow
aminokwasy
oznaczanie ilosciowe
electrophoresis
chromatography
bidirectional electrophoresis
electrochromatography
amino acid degradation
amino acid
quantitative determination
Źródło:
Roczniki Państwowego Zakładu Higieny; 1962, 13, 1
0035-7715
Pojawia się w:
Roczniki Państwowego Zakładu Higieny
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Isolation and characterization of zinc-binding proteins of canine seminal plasma
Autorzy:
Mogielnicka-Brzozowska, M.
Fraser, L.
Czarzasta, J.
Kordan, W.
Powiązania:
https://bibliotekanauki.pl/articles/32311.pdf
Data publikacji:
2012
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
isolation
zinc-binding protein
seminal plasma
dog
electrophoresis
reproductive process
Opis:
Zinc-binding proteins from seminal plasma (ZnBPs) originate in the secretions of different accessory sex glands and are implicated in key events associated with sperm-egg fertilization processes. This study describes the isolation and characterization of the ZnBPs of canine seminal plasma. Ejaculates were collected from three crossbred dogs for a 2-week period. The ZnBPs as well as non zinc-binding proteins (nZnBPs) were isolated by zinc-dependent affinity chromatography. The isolated fractions were subjected to native gel electrophoresis (one-dimensional polyacryamide gel electrophoresis, PAGE) and sodium dodecyl sulphate polyacryamide gel electrophoresis (SDS-PAGE), using denaturing and reducing conditions. Zinc-elution profile using affinity chromatography displayed two protein fractions represented by the nZnBPs and ZnBPs, respectively. Using native gel electrophoresis, it was found that both the nZnBPs and ZnBPs occurred in their native state as aggregates, ranging from 140 to 669 kDa. The nZnBPs were disaggregated into 8 protein bands, with molecular weights ranging from 10.7 to 79.7 kDa, following SDS-PAGE analysis. By contrast, SDS-PAGE analysis of the ZnBPs revealed 13 protein bands, with molecular weights ranging from 11.6 to 152.3 kDa. Densitometric analysis showed that 46-48% of nZnBPs could be accounted by protein fractions with molecular weights of 10.7 and 14.2 kDa. Also, 2 protein fractions with molecular weights of 11.6 and 14.3 kDa, were predominant in ZnBPs, accounting for approximately 28-30% of the total proteins. These results demonstrate the zinc-binding capacity of proteins secreted by the canine prostate. The findings of this study indicate that ZnBPs of canine seminal plasma comprise several protein fractions, which might be implicated in the reproductive processes in the dog.
Źródło:
Polish Journal of Veterinary Sciences; 2012, 15, 3
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Determination of plasma bone-specific alkaline phosphatase isoenzyme activity in Holstein calves using a commercial agarose gel electrophoresis kit
Autorzy:
Onomi, R.
Hatate, K.
Yamagishi, N.
Powiązania:
https://bibliotekanauki.pl/articles/2087395.pdf
Data publikacji:
2019
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
agarose gel electrophoresis (AGE)
bone-specific alkaline phosphatase isoenzyme
calf
Źródło:
Polish Journal of Veterinary Sciences; 2019, 4; 789-792
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Using capillary electrophoresis to study methylation effect on RNA-peptide interaction.
Autorzy:
Mucha, Piotr
Szyk, Agnieszka
Rekowski, Piotr
Agris, Paul
Powiązania:
https://bibliotekanauki.pl/articles/1043465.pdf
Data publikacji:
2003
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
methylated nucleosides
methylation
arginine methylation
RN-peptide interaction
capillary electrophoresis
Opis:
Methylation of RNA and proteins is one of a broad spectrum of post-transcriptional/translational mechanisms of gene expression regulation. Its functional signification is only beginning to be understood. A sensitive capillary electrophoresis mobility shift assay (CEMSA) for qualitative study of the methylation effect on biomolecules interaction is presented. Two RNA-peptide systems were chosen for the study. The first one consists of a 17-nucleotide analogue (+27-+43) of the yeast tRNAPhe anticodon stem and loop domain (ASLPhe) containing three of the five naturally occurring modifications (2'-O-methylcytidine (Cm32), 2'-O-methylguanine (Gm34) and 5-methylcytidine (m5C40)) (ASLPhe-Cm32,Gm34,m5C40) and a 15-amino-acid peptide (named tF2 : Ser1-Ile-Ser-Pro-Trp5-Gly-Phe-Ser-Gly-Leu10-Leu- Arg-Trp-Ser-Tyr15) selected from a random phage display library (RPL). A peptide-concentration-dependent formation of an RNA-peptide complex was clearly observable by CEMSA. In the presence of the peptide the capillary electrophoresis (CE) peak for triply methylated ASLPhe shifted from 18.16 to 20.90 min. Formation of the complex was not observed when an unmethylated version of ASLPhe was used. The second system studied consisted of the (+18)-(+44) fragment of the trans-activation response element of human immunodeficiency virus type 1 (TAR RNA HIV-1) and a 9-amino-acid peptide of the trans-activator of transcription protein (Tat HIV-1) Tat(49-57)-NH2 (named Tat1 : Arg49-Lys-Lys-Arg52-Arg-Gln-Arg-Arg- Arg57-NH2). In the presence of Tat(49-57)-NH2 a significant shift of migration time of TAR from 18.66 min to 20.12 min was observed. Methylation of a residue Arg52→Arg(Me)2, crucial for TAR binding, strongly disrupted formation of the complex. Only at a high micromolar peptide concentration a poorly shaped, broad peak of the complex was observed. CE was found to be an efficient and sensitive method for the analysis of methylation effects on interaction of biomolecules.
Źródło:
Acta Biochimica Polonica; 2003, 50, 3; 857-864
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Trypsin inhibitor electrophoretic patterns in Vicia faba L. and related species
Autorzy:
Zimniak-Przybylska, Z
Przybylska, J.
Krajewski, P.
Powiązania:
https://bibliotekanauki.pl/articles/2043622.pdf
Data publikacji:
1999
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
Vicia faba
trypsin inhibitor
Narbonensis
electrophoresis
faba bean
Bithynicae
taxonomy
Źródło:
Journal of Applied Genetics; 1999, 40, 4; 283-292
1234-1983
Pojawia się w:
Journal of Applied Genetics
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Sample Preparation for Determination of Biological Thiols by Liquid Chromatography and Electromigration Techniques
Autorzy:
Bald, Edward
Powiązania:
https://bibliotekanauki.pl/articles/896289.pdf
Data publikacji:
2004
Wydawca:
Uniwersytet Łódzki. Wydawnictwo Uniwersytetu Łódzkiego
Tematy:
sample preparation
liquid chromatografy
capillary electrophoresis
chemical derivatization
biological thiols
Opis:
Wydrukowano z dostarczonych Wydawnictwu UŁ gotowych materiałów
Majority of the bioanalytical or environmental methods do not use just one chromatografie or electrophoretic step, but rather involve several sample pretreatment steps which simplfy the matrix, and often preconcentrate and chemically modify the analytes. This work surveys typical procedures for sample preparation for most commonly analyzed biofluids with particular emphasis placed on chemical derivatization of sulfur amino acids for their determination by liquid phase separation techniques. Recent author's laboratory contribution to the development of sample preparation procedures is merked.
Źródło:
Acta Universitatis Lodziensis. Folia Chimica; 2004, 13
0208-6182
Pojawia się w:
Acta Universitatis Lodziensis. Folia Chimica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Proces impregnowania teflonem powłoki tlenkowej wytwarzanej metodą anodowego utleniania na podłożu aluminium
The Teflon impregnation of anodic coating onto aluminium substrate
Autorzy:
Kwolek, P.
Górecka, P.
Obłój, A.
Kwolek, U.
Dychtoń, K.
Drajewicz, M.
Sieniawski, J.
Powiązania:
https://bibliotekanauki.pl/articles/1208784.pdf
Data publikacji:
2016
Wydawca:
Stowarzyszenie Inżynierów i Techników Przemysłu Chemicznego. Zakład Wydawniczy CHEMPRESS-SITPChem
Tematy:
anodowanie
teflon
zużycie ścierne
korozja
elektroforeza
anodizing
abrasion resistance
corrosion
electrophoresis
Opis:
Powłoki tlenkowe wytworzone na podłożu aluminium o czystości technicznej impregnowano w procesie elektroforezy. Stosowano zawiesinę wodną cząstek teflonu. Ustalono wpływ wartości napięcia elektrycznego i czasu prowadzenia procesu elektroforezy na odporność na zużycie – w warunkach tarcia wytwarzanych impregnowanych powłok tlenkowych. Wykazano, że aluminium nieimpregnowane ma największą odporność na ścieranie. Proces impregnowania poprawia natomiast odporność korozyjną powłoki tlenkowej w roztworze HCl.
Electrophoresis was employed in order to impregnate anodic coatings obtained onto aluminium. The water suspension composed of teflon particles was applied. The influence of voltage and time of electrophoresis on the abrasion resistance of the impregnated coatings was determined. It was shown that anodized aluminium is the most wear resistant coating. However, the impregnation process enhances the corrosion resistance of anodic coatings in the HCl solution.
Źródło:
Chemik; 2016, 70, 7; 361-368
0009-2886
Pojawia się w:
Chemik
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Analysis of Genetic Similarities Between Clostridium Perfringens Isolates Isolated from Patients with Gas Gangrene and from Hospital Environment Conducted with the use of the PFGE Method
Autorzy:
Brzychczy-Włoch, Monika
Bulanda, Małgorzata
Powiązania:
https://bibliotekanauki.pl/articles/1395730.pdf
Data publikacji:
2014-03-01
Wydawca:
Index Copernicus International
Tematy:
Clostridium perfringens
gas gangrene
pulsed field gel electrophoresis - PFGE
epidemiological investigation
Opis:
The objective of the study was to perform a comparative analysis of genetic similarity, with the use of pulsed field gel electrophoresis (PFGE), of Clostridium perfringens isolates originating from patients with gas gangrene and from the hospital environment. The study encompassed two patients with a clinical and microbiological diagnosis of gas gangrene, who were hospitalized in one of the hospitals of the Małopolska province in the time period between 31st March 2012 and 18th May 2012. Clostridium perfringens isolates genotyping indicated that the isolates originating from the two studied patients did not display genetic similarity and represented two different PFGE types, which corresponded to two different clones (clone A and B). Whereas the strains isolated from the hospital environment were genetically identical with the strain coming from the second patient and represented one PFGE type, which corresponded to one clone (clone A). As a result of the study, it is possible to conclude that both patients developed endogenous infection. Even so, the examination of the hospital environment indicates the possibility of the appearance of exogenous infections. It prompts recommending and following the exact regulations of sanitary regime in the ward and the operating theater if a patient is diagnosed with gas gangrene.
Źródło:
Polish Journal of Surgery; 2014, 86, 3; 141-146
0032-373X
2299-2847
Pojawia się w:
Polish Journal of Surgery
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Polymorphism of the storage proteins in rye cultivars and populations selected for tolerance to nutrient deficiency.
Autorzy:
Rzepka-Plevneš, Danuta
Smolik, Miłosz
Powiązania:
https://bibliotekanauki.pl/articles/2198912.pdf
Data publikacji:
2003-12-21
Wydawca:
Instytut Hodowli i Aklimatyzacji Roślin
Tematy:
differences
electrophoresis
in vitro cultures
nutrient deficit
rye
storage protein
tolerance
Opis:
Ten open-pollinated cultivars, two strains of rye and Sl, S2, S3 progenies obtained after selection directed towards tolerance to nutrient deficit were used in the study. The aim of the study was to compare the genotypes of the above mentioned rye populations by electrophoretically characterised of their secalin patterns.The result showed that most of the tested rye cultivars revealed simi1ar but not identica1 electrophoretic patterns. Specific secalin patterns were obtained from all the cultivars, except cv. Arant and strain SMH 92. Differences in comparison to initial genotypes were observed in some rye populations in Sl and S2 generations, including additional polypeptide of molecular weight 43 kDa. It was not linked with rye tolerance to nitrogen and potassium deficit in medium.
Źródło:
Plant Breeding and Seed Science; 2003, 48; 49-59
1429-3862
2083-599X
Pojawia się w:
Plant Breeding and Seed Science
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Comparison of the effects of bleomycin and ionizing radiation in two sublines of murine lymphoma L5178Y
Autorzy:
Kruszewski, M.
Zaim, J.
Grądzka, I.
Szumiel, I.
Powiązania:
https://bibliotekanauki.pl/articles/147561.pdf
Data publikacji:
2001
Wydawca:
Instytut Chemii i Techniki Jądrowej
Tematy:
bleomycin
comet assay
DNA damage
gamma radiation
single cell gel electrophoresis
Opis:
We compared the effects of bleomycin (BLM) and ionizing radiation on two sublines of murine lymphoma L5178Y (LY): LY-R, radiation resistant and LY-S, radiation sensitive. This radiosensitivity difference is related to the ability to rejoin DNA double strand breaks. LY-S cells were about two times more sensitive to BLM than LY-R, similarly as in the case of sensitivity to X rays. Since there was no difference in the P-glycoprotein-related drug transport system between the sublines, it could be expected that the enhanced sensitivity of LY-S cells to BLM was caused by the DNA repair defect. Growth disturbances in BLM treated cell populations were proportional to the lethal effect and their duration was observed until elimination of dead cells (3-6 days after 50 ěM BLM, 1 h at 37oC). There was no slow growth phase accompanied by normal viability, as previously described for X-irradiated LY-S cells. Initial DNA damage, estimated with the single cell gel electrophoresis method was linearly related to BLM dose in LY-S cells; in LY-R cells - in the low dose range (up to 10 ěM) - there was more damage than in LY-S cells, however, at higher doses the dose - effect curves became identical. The doseeffect relationship for ă rays was linear and identical in both cell sublines. DNA damage distribution in BLM treated cells was much less uniform as compared to that in irradiated cells and indicated the presence of cells with severely damaged DNA, a feature typical for BLM action in vitro.
Źródło:
Nukleonika; 2001, 46, 3; 81-86
0029-5922
1508-5791
Pojawia się w:
Nukleonika
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Express analysis of chitosan and its derivatives by gel electrophoresis
Autorzy:
Konovalova, Mariya
Shagdarova, Balzhima Ts.
Zubov, Vitaliy
Svirshchevskaya, Elena
Powiązania:
https://bibliotekanauki.pl/articles/1034210.pdf
Data publikacji:
2019
Wydawca:
Sieć Badawcza Łukasiewicz - Polskie Towarzystwo Chitynowe
Tematy:
agarose gel electrophoresis
chitosan
chitosan derivatives
degree of deacetylation
molecular weight
Opis:
Chitosan is one of the most promising polymers for biomedical applications due to its unique properties, such as its biocompatibility, low toxicity, biodegradation, and the presence of reactive amino and hydroxyl groups. Analysis of physicochemical properties of chitosan and its derivatives is a time-consuming process and requires expensive equipment and large amounts of the sample. This paper proposes a method for express analysis of the molecular weight (MW), the degree of deacetylation (DD), the substitution degree (SD), and the charge of chitosan and its derivatives using agarose gel electrophoresis under acidic and neutral conditions with Coomassie staining. Positively charged chitosan samples required acidic Tris Acetate-EDTA (TAE) buffer to move in the gel. The electrophoretic mobility of chitosan depended on MW, DD, SD, and the chitosan charge. Based on the dependences obtained by the proposed method, the MW and DD of commercial chitosan samples were determined. Express analysis of chitosan and its derivatives in agarose gel can be used to monitor the reactions of chitosan modification and to analyse samples with unknown characteristics.
Źródło:
Progress on Chemistry and Application of Chitin and its Derivatives; 2019, 24; 84-95
1896-5644
Pojawia się w:
Progress on Chemistry and Application of Chitin and its Derivatives
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Inheritance of some w-gliadin protein subunits in spelt wheat and their linkage with the red glume coding Rg-1 locus.
Autorzy:
Waga, Jacek
Powiązania:
https://bibliotekanauki.pl/articles/2198843.pdf
Data publikacji:
2002-12-22
Wydawca:
Instytut Hodowli i Aklimatyzacji Roślin
Tematy:
A PAGE electrophoresis
linkage analysis
protein blocks
gliadin subunits
glume colour
Opis:
The Gli B1 locus controlling the synthesis of certain fractions of wheat storage proteins belonging to the group of ω-gliadins is tightly linked with the Rg1 gene responsible for red glume colour. The inheritance of selected ω-gliadin subunits and of glume colour was studied in order to identify markers of this trait among protein fractions specific to spelt wheat. Electrophoretic analysis for 217 single plants of the F2 generation in the cross Tr. aestivum (cultivar Elena) × Tr. spelta was done. Four out of five protein subunits specific for spelt wheat (O1, O3, O4 and O5) as well as gliadin block Gli B1-1 of the Elena cultivar were inherited according to Mendelian segregation ratio 1:2:1 assumed for two allelic protein variants of one gene. The above mentioned fractions of spelt wheat formed a block, which has been denoted by the symbol Gli B1-6. The results of segregation analysis regarding glume colour indicate Mendelian, single-gene inheritance of this trait (3:1 ratio of red and white glumes), which differs from the results of other investigations suggesting the existence of two genes controlling the red colour. The genetic distance between loci Gli B1 and Rg1 has been estimated to be 1.9 cM.
Źródło:
Plant Breeding and Seed Science; 2002, 46, 2; 25-35
1429-3862
2083-599X
Pojawia się w:
Plant Breeding and Seed Science
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Preparation of mares colostrum and milk sample for 2-DE separation without acetone precipitation
Przygotowanie próbek siary i mleka klaczy do rozdziału 2-DE, z pominięciem precypitacji acetonem
Autorzy:
Cichy, A.
Dratwa-Chałupnik, A.
Medeńska, W.
Ożgo, M.
Pikuła, R.
Powiązania:
https://bibliotekanauki.pl/articles/2621587.pdf
Data publikacji:
2020
Wydawca:
Zachodniopomorski Uniwersytet Technologiczny w Szczecinie. Wydawnictwo Uczelniane ZUT w Szczecinie
Tematy:
electrophoretic separation
colostrum
mare milk
proteomic analysis
precipitation
acetone
2-D electrophoresis
Źródło:
Acta Scientiarum Polonorum. Zootechnica; 2020, 19, 3; 71-77
1644-0714
Pojawia się w:
Acta Scientiarum Polonorum. Zootechnica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Zastosowanie elektroforezy kapilarnej do oznaczania wybranych jonów nieorganicznych w pozostałościach po przemianie wybuchowej materiałów pirotechnicznych
Application of capillary electrophoresis for selected inorganic ions determination after explosive transformation of pyrotechnic materials
Autorzy:
Wideł, Dariusz
Smagacz, Łukasz
Klepacz, Michał
Powiązania:
https://bibliotekanauki.pl/articles/1366460.pdf
Data publikacji:
2019
Wydawca:
Centralny Ośrodek Badawczo-Rozwojowy Aparatury Badawczej i Dydaktycznej, COBRABiD
Tematy:
materiały pirotechniczne
elektroforeza kapilarna
jony nieorganiczne
pyrotechnic materials
capillary electrophoresis
inorganic anions
Opis:
Zastosowano metodę elektroforezy kapilarnej (CE) do oznaczenia jakościowego i ilościowego jonów: ClO3-, ClO4-, NO3-, NH4+,K+ w pozostałościach po przemianie wybuchowej materiałów pirotechnicznych.Po wybuchu petardy hukowej FP3 i petardy wojskowej pobrano próbki gleby z miejsca wybuchu i poddano je ekstrakcji, a następnie analizie metodą CE. Wyznaczone granice oznaczalności wynosiły od 0,78 mg/l dla K+ do 3,12 dla NO-. Metoda CE pozwoliła na rozróżnienie materiałów pirotechnicznych na podstawie oznaczenia wybranych jonów w pozostałościach po wybuchu.
Capillary electrophoresis (CE) method was applied for qualitative and quantitative determination ClO3-, ClO4-, NO3-, NH4+,K+ , in pyrotechnic materials after explosive transformation. After ex- plosion of fire-cracker with black powder and military fire-cracker, soil samples from explosion area were collected, extracted and analyzed by CE method. Determined limits of quantifications were from 0.78 mg/L for K+ to 3.12 for NO-. The CE method allowed to distinguish pyrotechnic materials on the basis of selected ions determination in pyrotechnic materials residues.
Źródło:
Aparatura Badawcza i Dydaktyczna; 2019, 24, 2; 179-184
2392-1765
Pojawia się w:
Aparatura Badawcza i Dydaktyczna
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Elektroforeza swobodna z przepływem hydrodynamicznym : podstawy teoretyczne i rozwiązania techniczne
Free flow electrophoresis : theory and technology
Autorzy:
Hoda, P.
Polak, B.
Dzido, T. H.
Powiązania:
https://bibliotekanauki.pl/articles/172756.pdf
Data publikacji:
2018
Wydawca:
Polskie Towarzystwo Chemiczne
Tematy:
elektroforeza swobodna
przepływ hydrodynamiczny
technika elektromigracyjna
rozdzielanie pseudo-dwuwymiarowe
rozdzielanie preparatywne
mikro-elektroforeza swobodna
free flow electrophoresis
electromigration technique
pseudo-two dimensional separation
preparative separation
micro free flow electrophoresis
Opis:
Free flow electrophoresis, or carrier-free electrophoresis, is a technique based upon the principle of mobility of chargeable species in the electric field. However, unlike more widespread gel electrophoresis, free flow electrophoresis does not utilise any kind of solid support medium. Instead, species to be separated move within the space filled with aqueous buffer, which is constantly pumped in the direction perpendicular to the direction of the applied electric field. Such set-up allows for continuous separation, as compounds of the sample being processed form separate bands and leave the separation system through several different outlets located along the edge of the chamber. Furthermore, the use of mild separation conditions increases the chance of biologically active compounds retaining their activity after the separation is finished. These qualities make free flow electrophoresis an excellent tool for protein research and cytology. In this review, the basic theoretical aspects of the technique are outlined, with a special emphasis placed on various modes in which free flow electrophoresis can be operated. Besides, a review of milestone papers related to free flow electrophoresis technology is presented. Apart from the devices following the original concept of Barrollier and Hannig, an insight into the construction of recirculating instrumentation, multicompartment electrolysers as well as radially-symmetric chambers is provided. A special focus is placed on patents and commercialized solutions. Finally, the challenges of scaling-down free flow electrophoresis to micro-dimensions are introduced.
Źródło:
Wiadomości Chemiczne; 2018, 72, 1-2; 39-60
0043-5104
2300-0295
Pojawia się w:
Wiadomości Chemiczne
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Proteome responses to herbicidal stress in maize
Autorzy:
Tyczewska, A.
Bielinska, I.
Gracz, J.
Sikora, M.
Twardowski, T.
Powiązania:
https://bibliotekanauki.pl/articles/951291.pdf
Data publikacji:
2015
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
abiotic stress
plant response
maize
proteomic analysis
gene expression
two dimensional gel electrophoresis
Źródło:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2015, 96, 1
0860-7796
Pojawia się w:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Proteins and enzymatic systems in three varieties of Helianthus annuus L.
Autorzy:
Kalinowski, A
Klimko, M.
He, L.
Kluza, M.
Powiązania:
https://bibliotekanauki.pl/articles/878224.pdf
Data publikacji:
2001
Wydawca:
Uniwersytet Przyrodniczy w Poznaniu
Tematy:
variability
Helianthus annuus
enzyme
hybrid
electrophoresis
protein
enzyme system
oil plant
isoelectric focusing
Opis:
The paper reports results of analysis of protein and eight enzymatic systems of three varieties of Helianthus annuus L. (Wielkopolski, Coril, and Frankasol) performed by three electrophoretic methods. Electrophoretic analyses allowed finding protein and isoenzymes markers for each of variety. The two interline hybrids Coril and Frankasol shared more features in common than the population variety Wielkopolski with each of the former. Mean values of the polymorphism index for the protein and the eight enzymes indicate that the variety Wielkopolski is more homogeneous than Coril and Frankasol.
W pracy przedstawiono wyniki analiz białek i ośmiu układów enzymatycznych, rozdzielanych trzema metodami elektroforezy, u trzech odmian Helianthus annuus L. (Wielkopolski, Coril i Frankasol). Elektroforetyczne analizy umożliwiły znalezienie białkowych i izoenzymatycznych markerów dla trzech odmian. U dwóch odmian międzyliniowych mieszańców Coril i Frankasol wykryto więcej cech wspólnych niQ u wymienionych odmian i odmiany Wielkopolski. Średnie wartości indeksu polimorfizmu, obliczone dla białek i ośmiu układów enzymatycznych, pokazały, że odmiana Wielkopolski była bardziej wyrównana niż odmiany Coril i Frankasol.
Źródło:
Roczniki Akademii Rolniczej w Poznaniu. Botanika; 2001, 04
1508-9193
Pojawia się w:
Roczniki Akademii Rolniczej w Poznaniu. Botanika
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
A proteomics approach to identify the differential protein level in cardiac muscle of diabetic rat
Autorzy:
Karthik, Dhanaraj
Vijayakumar, Ravichandran
Pazhanichamy, Kalailingam
Ravikumar, Sivanesan
Powiązania:
https://bibliotekanauki.pl/articles/1039290.pdf
Data publikacji:
2014
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
diabetes mellitus
cardiac muscle proteome
2D electrophoresis
MALDI-TOF-MS
phylogenetic analysis
Opis:
Background: Cardiovascular proteomics investigation reveals the characterization and elucidation of the novel therapeutic targets and strategies to prevent the development of heart failure associated diabetic complication by using 2DE and MS. Methods: The experimental animals were made diabetic with a single intraperitoneal injection of alloxan (150 mg/kg of bw). Albino rats were randomly divided into four individual groups: Group-I control (n=6), group-II alloxan-induced diabetic rats, untreated (n=6), group-III (n=6) and group-IV (n=6) alloxan-induced diabetic rats were treated with aqueous and ethanolic extracts of Cynodon dactylon for 15 days, respectively. Animals were euthanized to collect the heart tissues and blood samples. 2DE sample preparation, gel running and staining (n=6: each groups) were performed at the same time to avoid variation. The result of six gel images from each group were analyzed and evaluated as one match set with 2D software (P<0.05). Results: The above experiment revealed two up-regulated proteins in group-II i.e. NTF4 and ETFB. Conclusions: NTF4 is a neuro-protective agent for neuro-degenerative diseases. It will prevent diabetic secondary complications, such as diabetic polyneuropathy and cardiomyopathy. ETFB is active in the mitochondria, the energy-producing centres in cells. It is clear from the experiment that because of up-regulation of ETFB more energy is availabile and the electron transfer for heart during diabetes is possible, what leads to reduce the oxidative stress and free-radical formation. The up-regulated proteins reduced CVD that occurred just before overt hyperglycaemia due to administration of C. dactylon. This approach established the preliminary reference map for decoding cellular mechanisms linked between pathogenesis CVD and diabetes.
Źródło:
Acta Biochimica Polonica; 2014, 61, 2; 285-293
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Molecular basis of cellulose biosynthesis disappearance in submerged culture of Acetobacter xylinum
Autorzy:
Krystynowicz, Alina
Koziołkiewicz, Maria
Wiktorowska-Jezierska, Agnieszka
Bielecki, Stanisław
Klemenska, Emilia
Masny, Aleksander
Płucienniczak, Andrzej
Powiązania:
https://bibliotekanauki.pl/articles/1041378.pdf
Data publikacji:
2005
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
2-D electrophoresis
UDP-glucose pyrophosphorylase
phosphoglucomutase
bacterial cellulose
Acetobacter xylinum
PCR-MP
Opis:
Acetobacter xylinum strains are known as very efficient producers of bacterial cellulose which, due to its unique properties, has great application potential. One of the most important problems faced during cellulose synthesis by these bacteria is generation of cellulose non-producing cells, which can appear under submerged culture conditions. The reasons of this remain unknow. These studies have been undertaken to compare at the molecular level wild-type, cellulose producing (Cel+) A. xylinum strains with Cel- forms of cellulose-negative phenotype. Comparison of protein profiles of both forms of A. xylinum by 2D electrophoresis allowed for the isolation of proteins which were produced exclusively by either Cel+ or Cel- cells. Sequences of peptides derived from these proteins were aligned with those of proteins deposited in databases. This analysis revealed that Cel- cells lacked two enzymes: phosphoglucomutase and glucose-1-phosphate uridylyltransferase, which generates UDP-glucose being the substrate for cellulose synthase. DNA was analyzed by ligation-mediated PCR carried out at low denaturation temperature (PCR-MP). Two DNA fragments of different thermal stability (218 and 217 bp) were obtained from the DNA of Cel+ and Cel- forms, respectively. The only difference between these Cel- and Cel+ DNA fragments is deletion of one T residue. Alignment of those two sequences with those deposited in the GenBank database revealed that similar fragments are present in the genomes of some bacterial cellulose producers and are located downstream from open reading frames (ORF) encoding phosphoglucomutase. The meaning of this observation is discussed.
Źródło:
Acta Biochimica Polonica; 2005, 52, 3; 691-698
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Urinary proteomic strategies in biomarkers discovery of renal diseases
Autorzy:
Ciechanowicz, A.K.
Ozgo, M .
Herosimczyk, A.
Kurpinska, A.
Klonowska, A.
Lepczynski, A.
Stanski, L.R.
Powiązania:
https://bibliotekanauki.pl/articles/3155.pdf
Data publikacji:
2011
Wydawca:
Instytut Medycyny Wsi
Tematy:
proteomics
biomarker discovery
human disease
renal disease
chromatography
electrophoresis
mass spectrometry
kidney
urine
Źródło:
Journal of Pre-Clinical and Clinical Research; 2011, 05, 1
1898-2395
Pojawia się w:
Journal of Pre-Clinical and Clinical Research
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Identification of mare colostrum proteins
Identyfikacja bialek siary klaczy – badania wstępne
Autorzy:
Medeńska, W.
Dratwa-Chałupnik, A.
Ożgo, M.
Cichy, A.
Pikuła, R.
Bobik, J.
Powiązania:
https://bibliotekanauki.pl/articles/3131472.pdf
Data publikacji:
2020
Wydawca:
Zachodniopomorski Uniwersytet Technologiczny w Szczecinie. Wydawnictwo Uczelniane ZUT w Szczecinie
Tematy:
colostrum
mare
proteomics
animal feeding
functional protein
foal growth
development
two-dimensional electrophoresis
Źródło:
Acta Scientiarum Polonorum. Zootechnica; 2020, 19, 4; 25-31
1644-0714
Pojawia się w:
Acta Scientiarum Polonorum. Zootechnica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Primary results of 2-dimensional electrophoresis for protein studies of Gentiana kurroo Royle somatic embryos derived from long-term embryogenic cell suspensions
Autorzy:
Niedziela, A.
Rybczynski, J.J.
Powiązania:
https://bibliotekanauki.pl/articles/56507.pdf
Data publikacji:
2014
Wydawca:
Polskie Towarzystwo Botaniczne
Tematy:
two dimensional gel electrophoresis
protein
Gentiana kurroo
somatic embryo
embryogenic cell
cell suspension
Opis:
Two-dimensional gel electrophoresis was used to compare protein expression profiles between various stages of Gentiana kurroo Royle somatic embryos. Seven distinct stages (I–VII) were pointed out and measured from long-term embryogenic cell suspension. Isoelectric focusing was done in the pH intervals 3–10, and the second dimension was carried out with 13% SDS-polyacrylamide gel electrophoresis. Dependent on the stage from about 400 (stage IV) to more than 700 (stage II) protein spots were totally detected. The molecular weight of abundant proteins range from 12 to 70 kDa, however, majority of proteins were located between 20–49 kDa spots on the gels. The highest difference in the number of spots appeared in the case of globular embryo (stage I) and elongated cotyledonary stage (stage VII) with differences being about 130 spots. The relevance of embryogenic cell suspension choice for proteomic analysis as well as expediency of the increasing number of particular embryo stages is discussed.
Źródło:
Acta Societatis Botanicorum Poloniae; 2014, 83, 3
0001-6977
2083-9480
Pojawia się w:
Acta Societatis Botanicorum Poloniae
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Związek białek zapasowych z zimotrwałością u wybranych mieszańców pszenicy ozimej
Relationship between wheat storage proteins and winter hardiness in chosen hybrids of winter wheat
Autorzy:
Witkowska, Krystyna
Witkowski, Edward
Śmiałowski, Tadeusz
Waga, Jacek
Powiązania:
https://bibliotekanauki.pl/articles/2198492.pdf
Data publikacji:
2013-06-30
Wydawca:
Instytut Hodowli i Aklimatyzacji Roślin
Tematy:
elektroforeza
markery biochemiczne
pszenica ozima
zimotrwałość
electrophoresis
bichemical markers
winter wheat
winter hardiness
Opis:
Celem badań było poszukiwanie związków pomiędzy markerami biochemicznymi a stopniem przezimowania u 2 grup pszenic ozimych: 19 wieloliniowych genotypów (90 linii F5) pochodzących z 6 kombinacji mieszańcowych pszenicy zwyczajnej (T. aestivum ssp. vulgare) oraz trzech par genotypów wytworzonych z mieszańca orkiszu (odmiany Oberkummer Rotkorn (Triticum aestivum spelta L.) i rodu LAD 480 (T. aestivum ssp. vulgare). Materiał poddano analizom elektro¬foretycznym. W pierwszej grupie badanego materiału stwierdzono silne zróżnicowanie pod względem zimotrwałości wahające się od 4 do 9 stopni (w skali 9°). Uzyskane wyniki sugerują, że kombinacja podjednostek HMW glutenin [Glu A1(Null), Glu B1(7+9), Glu D1 (5+10)] jest powiązana z podwyższoną zimotrwałością pszenicy ozimej. Zimotrwałość drugiej grupy mieszańców oddalonych była niższa i wahała się od 1 do 5 stopnia. Markerami wysokiej zimotrwałości u tej grupy mieszańców okazał się blok białek gluteninowych z podjednostką HMW warunkowanych przez locus Glu A1 z podjednostką „2”i locus Glu D1 z podjednostką „5+10/2+12” oraz blok białek gliadynowych z podjednostkami zawierającymi markery białkowe linii z null allelem w locus Gli B2.
The aim of these studies was to find out whether polymorphic wheat storage proteins (gliadins and HMW glutenins) and winter hardiness are interrelated. Two sets of winter wheats were analyzed. In the first set 19 groups of hybrid genotypes (90 lines in total) of F5 generation from cross combinations between common wheat (Triticum aestivum ssp. vulgare) cultivars and strains were analyzed. The second set comprised four pairs of hybrid genotypes originating from crossing between spelt (Triticum aestivum ssp. spelta, cv. Obercummer Rotkorn) and common wheat breeding strain LAD 480. Gliadins and HMW glutenins extracted from individual kernels of analyzed hybrid genotypes were separated using electrophoretic techniques: A-PAGE and SDS-PAGE. The obtained results suggested close relationships between HMW glutenin subunits: Glu A1-1, Glu B1-7+9/6+8, Glu D1-5+10 and gliadin null alleles localized on the chromosome 6B (locus Gli B2) and winter hardiness of winter wheat.
Źródło:
Biuletyn Instytutu Hodowli i Aklimatyzacji Roślin; 2013, 268; 23-34
0373-7837
2657-8913
Pojawia się w:
Biuletyn Instytutu Hodowli i Aklimatyzacji Roślin
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Elektroforetyczna identyfikacja proteinaz cysteinowych w surowicach pacjentów z przewlekłą białaczką limfocytową (PBL) z wykorzystaniem biotynylowanego jodoacetamidu
Electrophoretic method of cysteine proteinase identification in the sera of patients with chronic lymphocytic leukemia (CLL) based on biotinylated iodoacetamide
Autorzy:
Młudzik, Paulina
Pietrzak, Jacek
Saed, Lias
Wodziński, Damian
Franiak–Pietryga, Ida
Mirowski, Marek
Powiązania:
https://bibliotekanauki.pl/articles/1032608.pdf
Data publikacji:
2016
Wydawca:
Łódzkie Towarzystwo Naukowe
Tematy:
proteinazy cysteinowe
jodoacetamid
elektroforeza
przewlekła białaczka limfocytowa
cysteine proteases
iodoacetamide
electrophoresis
chronic
lymphocytic leukemia
Opis:
Wstęp: Proteinazy cysteinowe są enzymami regulującymi liczne procesy fizjologiczne oraz patologiczne w organizmie człowieka. Zaburzenie ich aktywności może przyczyniać się do wystąpienia wielu chorób. Pełnią one ważną rolę w procesie kancerogenezy, uczestnicząc w inwazji, transformacji nowotworowej, angiogenezie, apoptozie oraz powstawaniu przerzutów. Celem pracy było opracowanie elektroforetycznej metody identyfikacji proteinaz cysteinowych w surowicach pacjentów z przewlekłą białaczką w oparciu o jodoacetamid biotynylowany. Materiał i metody: Badania wstępne przeprowadzono na handlowym preparacie papainy (EC 3.4.22.2), dobrze poznanej i szeroko stosowanej roślinnej proteinazy cysteinowej o masie cząsteczkowej 23,4 kDa. Badania wykonano na próbach surowicy uzyskanych z pełnej krwi pobranej od pacjentów chorych na przewlekłą białaczkę limfocytową (PBL) oraz surowicach kontrolnych uzyskanych od dawców. Surowice po wstępnej inkubacji z jodoacetamidem mieszano z buforem do prób i poddawano rozdziałowi elektroforetycznemu w żelu poliakrylamidowym zawierającym dodecylosiarczan sodu (SDS–PAGE). Rozdzielone elektroforetycznie białka po transferze na nitrocelulozową membranę, poddawano dalszej analizie za pomocą streptawidyny skoniugowanej z peroksydazą chrzanową (HRP). Użycie substratu dla HRP, czterochlorowodorku 3,3’–diaminobenzydynyny (DAB), umożliwiało identyfikację biotynylowanego jodoacetamidu, a przez to związanych z nim w sposób nieodwracalny proteinaz cysteinowych obecnych w badanych surowicach. Wyniki: Analiza porównawcza surowic pobranych od pacjentów chorych na przewlekłą białaczkę limfocytową w odniesieniu do surowic kontrolnych pozwoliła na identyfikację dodatkowego białka o charakterze proteinazy cysteinowej o masie cząsteczkowej wynoszącej około 37 kDa, które nie występowało lub było obecne w niewielkiej ilości w surowicach kontrolnych. Wnioski: Opracowana metoda umożliwia wykrywanie proteinaz cysteinowych w surowicach kontrolnych, jak i u pacjentów chorych na przewlekłą białaczkę limfocytową.
Introduction: Cysteine proteases are enzymes that regulate numerous physiological and pathological processes in the human body. Disorders of their activity can lead to a number of diseases. They play an important role in the process of carcinogenesis, participating in the invasion, transformation, angiogenesis, apoptosis and metastasis. The aim of this study was to elaborate the electrophoretic method of cysteine proteinases identification in the sera of patients suffering from chronic lymphocytic leukemia based on biotinylated iodoacetamide. Material and methods: Preliminary studies were carried out on the commercially available papain (EC 3.4.22.2) well known and widely used plant cysteine protease with a molecular weight 23,4 kDa. The study was conducted on the blood samples taken from patients with chronic lymphocytic leukemia (CLL) and control sera from healthy donors. The sera after the preincubation with iodoacetamide were mixed with the sample buffer followed by electrophoresis on polyacrylamide gel containing sodium dodecyl sulfate (SDS–PAGE). The separated proteins were electrophoretically transferred to the nitrocellulose membranes and subjected to the further analysis using streptavidin conjugated with horseradish peroxidase (HRP). The use of substrate for HRP 3,3’- diaminobenzidine tetrachloride (DAB) allows the biotinylated iodoacetamide and thereby cysteine proteinase identification. Results: The comparative analysis of the sera from the patients with chronic lymphocytic leukemia and the control sera led to the identification of additional protein with a cysteine protease characteristic having a molecular weight of about 37 kDa, which did not occur or was present in a smaller amount of the control sera. Conclusions: The developed method allows the detection of cysteine proteases which are present in the control sera and the sera of patients with chronic lymphocytic leukemia.
Źródło:
Folia Medica Lodziensia; 2016, 43, 1; 35-55
0071-6731
Pojawia się w:
Folia Medica Lodziensia
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Analiza wydajności i specyficzności procesu immobilizacji syntetycznego inhibitora proteaz serynowych z zastosowaniem elektroforezy kapilarnej
Analysis of the efficiency and specificity of the synthetic serine protease inhibitor immobilization process using capillary electrophoresis
Autorzy:
Szałapata, K.
Osińska-Jaroszuk, M.
Grąz, M.
Jarosz-Wilkołazka, A.
Powiązania:
https://bibliotekanauki.pl/articles/2073100.pdf
Data publikacji:
2015
Wydawca:
Stowarzyszenie Inżynierów i Techników Mechaników Polskich
Tematy:
immobilizacja
inhibitor proteaz serynowych AEBSF
elektroforeza kapilarna
immobilization
AEBSF serine protease inhibitor
capillary electrophoresis
Opis:
Przedstawiono proces immobilizacji syntetycznego inhibitora proteaz serynowych AEBSF, który należy do rodziny związków benzosulfonowych. Zastosowanie techniki elektroforezy kapilarnej do celów analizy ilościowej pozwoliło na przeprowadzenie szybkiego pomiaru, charakteryzującego się wysoką specyficznością i precyzją. Na podstawie uzyskanych wyników stwierdzono, że inhibitor AEBSF, poddawany procesowi kowalencyjnej immobilizacji, jest efektywnie wiązany do porowatego nośnika. Jednocześnie określono, że wiąże się on z matrycą także wiązaniami adsorpcyjnymi i hydrofobowymi.
The paper describes a method of immobilization of AEBSF synthetic protease inhibitor which belongs to the family of benzensulfonyl compounds. The application of capillary electrophoresis for a quantitative analysis allows one to perform high-speed measurement characterized by high specificity and repeatability. The obtained results allowed one to confirm that the AEBSF inhibitor under covalent immobilization process is effectively bound to the porous support. It was also determined that AEBSF inhibitor binds to the matrix by adsorption and hydrophobic bonds.
Źródło:
Inżynieria i Aparatura Chemiczna; 2015, 3; 119--120
0368-0827
Pojawia się w:
Inżynieria i Aparatura Chemiczna
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Heterogeneity of methicillin-sensitive Staphylococcus pseudintermedius strains isolated from diseased dogs
Autorzy:
Garbacz, K.
Piechowicz, L.
Zarnowska, S.
Haras, K.
Dabrowska-Szponar, M.
Powiązania:
https://bibliotekanauki.pl/articles/32033.pdf
Data publikacji:
2011
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
animal disease
dog
heterogeneity
methicillin
Staphylococcus pseudintermedius
isolation
diseased animal
pulsed-field gel electrophoresis
Opis:
Thirty nine canine S. pseudintermedius strains were examined for antibiotic susceptibility and genetic polymorphisms. All strains were methicillin-sensitive S. pseudintermedius (MSSP). Resistance to penicillin was most prevalent (66.6%), followed by resistance to neomycin (56.4%), erythromycin (53.8%), clindamycin (48.7%), chloramphenicol (48.7%), and tetracycline (46.2%). Pulsed-field electrophoresis (PFGE) showed a high genetic polymorphism in the investigated strains.
Źródło:
Polish Journal of Veterinary Sciences; 2011, 14, 2
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Comparative proteomic analysis of Bombyx mori hemolymph and fat body after calorie restriction
Autorzy:
Chen, Huiqing
Li, Yijia
Chen, Keping
Yao, Qin
Li, Guohui
Wang, Lin
Powiązania:
https://bibliotekanauki.pl/articles/1040312.pdf
Data publikacji:
2010
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
calorie restriction
Bombyx mori
two-dimensional gel electrophoresis
proteomic analysis
MALDI-TOF/TOF MS
Opis:
Calorie restriction (CR) is known to extend life span from yeast to mammals. To gain an insight into the effects of CR on growth and development of the silkworm Bombyx mori at protein level, we employed comparative proteomic approach to investigate proteomic differences of hemolymph and fat body of the silkworm larvae subjected to CR. Thirty-nine differentially expressed proteins were identified by MALDI TOF/TOF MS. Among them, 19 were from the hemolymph and 20 from the fat body. The hemolymph of the CR group contained two down-regulated and 17 up-regulated proteins, whereas the fat body contained 15 down-regulated and five up-regulated ones. These proteins belonged to those functioning in immune system, in signal transduction and apoptosis, in regulation of growth and development, and in energy metabolism. Our results suggest that CR can alter the expression of proteins related to the above four aspects, implying that these proteins may regulate life span of the silkworm through CR.
Źródło:
Acta Biochimica Polonica; 2010, 57, 4; 505-511
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
DNA double-strand break rejoining in radioadapted human lymphocytes: evaluation by neutral comet assay and pulse-field gel electrophoresis
Autorzy:
Wojewódzka, M.
Buraczewska, I.
Szumiel, I.
Grądzka, I.
Powiązania:
https://bibliotekanauki.pl/articles/146153.pdf
Data publikacji:
2006
Wydawca:
Instytut Chemii i Techniki Jądrowej
Tematy:
human lymphocytes
radioadaptation
DNA double-strand break rejoining
neutral comet assay
pulsefield gel electrophoresis
Opis:
Adaptive response (AR), an enhanced resistance to a high dose of ionising radiation acquired after pretreatment with a very low dose, was estimated in normal human lymphocytes. The question posed was whether the extent of radioadaptation, assessed by micronucleus test, would be related to the rate of DNA double-strand break (DSB) rejoining. Phytohemagglutinin-stimulated G1-lymphocytes from 5 healthy male volunteers were pre-treated (or not) with an adaptive (5 cGy) dose of X-rays, followed by a higher (5 or 10 Gy) challenge dose after 20-22 h. DSB rejoining after the challenge dose was monitored with the use of two methods: neutral comet assay, modified to reduce the contribution of single-strand breaks (SSBs) and thermolabile sites, and pulse-field gel electrophoresis (PFGE), specific for DSBs. At the level of micronuclei, an AR was observed in lymphocytes of 3 of 5 donors. Up to 60 min, comet assay showed no statistically significant differences in DNA break rejoining between adapted and non-adapted lymphocytes, independently of AR appearance. PFGE gave similar results, although in three donors it revealed secondary increases in DSBs levels at 30 min and/or 60 min post-irradiation in the adapted vs. the non-adapted samples. Failure to demonstrate changes in DSBs rejoining rate in the adapted lymphocytes could be due to diversity of AR intensity/timing at the level of DNA repair in not fully homogenous cell populations. Also, “rare” DNA cuts characteristic of early apoptosis/necrosis could overlap the process of DNA break rejoining.
Źródło:
Nukleonika; 2006, 51, 4; 185-191
0029-5922
1508-5791
Pojawia się w:
Nukleonika
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Lunasin – a bioactive peptide from triticale (X Triticosecale Wittmack) seeds, inhibits proliferation of cancer HeLa and SK-OV-3 cells
Autorzy:
Galbas, M.E.
Porzucek, F.
Selwet, M.
Nowak, A.
Slomski, R.
Powiązania:
https://bibliotekanauki.pl/articles/80518.pdf
Data publikacji:
2017
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
triticale
Triticosecale
seed
bioactive peptide
lunasin
immunoblotting
proliferation
neoplastic cell
SDS-PAGE electrophoresis
HeLa cell
Źródło:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2017, 98, 3
0860-7796
Pojawia się w:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
SSCP polymorphism within 5 region of bovine lactoglobulin [LGB] gene
Autorzy:
Kaminski, S
Zabolewicz, T.
Powiązania:
https://bibliotekanauki.pl/articles/2044462.pdf
Data publikacji:
1998
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
lactoglobulin gene
polymorphism
SSCP method
cattle
gene transcription
electrophoresis
hormonal receptor
mutation
beta-lactoglobulin
milk
Opis:
In the paper the detection of the SSCP polymorphism within the 5’ fragment of bovine beta-lactoglobulin (LGB) gene is described. The 5’ fragment of LGB gene (209 bp) was PCR-amplified and then subjected to electrophoresis allowing the detection of SSCP polymorphism. Among 124 animals (50 cows and 74 bulls) six SSCP patterns were identified and named Rl, R2, R3, R4, R5 and R6, which occured with the frequency of 0.32, 0.51, 0.09, 0.06, 0.01 and 0.01, respectively. The PCR-SSCP method is simple, fast, and relatively inexpensive. The SSCP polymorphism reported in the paper may be useful in looking for the associations between different SSCP patterns and LGB gene expression and milk properties.
Źródło:
Journal of Applied Genetics; 1998, 39, 1; 97-102
1234-1983
Pojawia się w:
Journal of Applied Genetics
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Weryfikacja elektroforetyczna tożsamości roślin nietypowych pszenżyta ozimego z poletek kontroli następczej w Stacji Doświadczalnej Oceny Odmian w Zybiszowie
An electrophoretical technique to verify off-type plants of winter triticale during a post-control at the Experimental Station of Cultivars Testing in Zybiszów
Autorzy:
Drzewiecki, Jerzy
Mrówczyński, Adam
Powiązania:
https://bibliotekanauki.pl/articles/42834851.pdf
Data publikacji:
2009
Wydawca:
Instytut Hodowli i Aklimatyzacji Roślin
Tematy:
kontrola następcza
elektroforeza
prolaminy
rośliny nietypowe
tożsamość
electrophoresis
identity
off-type plants
post-control
prolamins
Opis:
W latach 2001, 2002, 2003 i 2004 w Stacji Doświadczalnej Oceny Odmian w Zybiszowie na poletkach kontroli następczej wysiano partie nasion z reprodukcji pięciu odmian pszenżyta ozimego. Najwięcej partii było w stopniu materiału kwalifikowanego pierwszego rozmnożenia (C/1) — 183. W sumie obsiano 297 poletek pszenżyta, w tym 75 odmianą Woltario, 73 Lamberto, 67 Fidelio, 47 Kitaro i 35 Magnat. Materiał do badań stanowiły rośliny nietypowe oraz zebrane z tych roślin ziarniaki, z lat zbioru 2002–2005. Celem pracy było oznaczenie roślin nietypowych oraz weryfikacja elektroforetyczna ich tożsamości na podstawie elektroforegramów prolamin monomerycznych ziarniaków. Wykonano obserwacje cech morfologicznych i fenologicznych roślin w fazie kłoszenia, przez porównanie z wzorcem. Tożsamość ziarniaków weryfikowano metodą elektroforezy prolamin monomerycznych ziarniaków, w żelu poliakrylamidowym, w środowisku kwaśnym (A-PAGE, pH 3). Odmiany Fidelio i Woltario miały najwięcej poletek z roślinami nietypowymi (ponad 70%), a odmiany Lamberto i Kitaro miały ich najmniej (około 1/3). Najczęściej obserwowanymi cechami roślin nietypowych były „rośliny wyższe”, „rośliny wcześniejsze” i „rośliny bez owłosienia dokłosia”. Weryfikacja elektroforetyczna potwierdziła prawidłowość oznaczeń poletkowych większości roślin nietypowych. Rośliny nietypowe u odmiany Kitaro stanowiły prawie 96,9%, które zweryfikowano jako „inne”. Ponad połowa roślin nietypowych Fidelio, Woltario i Magnat, (odpowiednio — 80,1%, 66,9% i 55,5%, ale u Lamberto tylko 36,1%) zweryfikowano jako „inne”. Najwyższy odsetek roślin nietypowych o niepotwierdzonej tożsamości stwierdzono dla cechy „rośliny wyższe”, a niższy odsetek dla cech „rośliny wcześniejsze” i „rośliny bez owłosienia dokłosia”.
Seed lots of five winter triticale cultivars were sown in post-control plots at the Experimental Station of Cultivars Testing in Zybiszów in the years 2001, 2002, 2003 and 2004. Certified seeds of the 1st generation (C/1) were predominant (183) among all the lots. In total, 297 experimental plots including 75, 73, 67, 47 and 35 plots for cvs Woltario, Lamberto, Fidelio, Kitaro and Magnat, respectively, were set up. The object of the investigations were untypical (off-type) plants and kernels harvested from these plants in the years 2002, 2003, 2004 and 2005. The aim of the work was to identify off-type plants and to verify their identity based on electrophoregrams of prolamins extracted from grains. Morphological and phenological traits of the plants were determined and compared with those of plants in reference plots. An electrophoretical analysis to verify the identity of off-type plants was done by A-PAGE, pH 3, electrophoresis. The greatest number of plots in which off-type plants were identified was recorded with cvs Fidelio and Woltario (>70%), whereas the smallest one was found with cvs Lamberto and Kitaro (approx. 1/3 of plots). Predominant among the off-type plants were the following traits: “higher plants”, “earlier earing” and “no hairiness of neck”. The identity of most of the examined grains produced by the off-plants was confirmed. In cv. Kitaro, the identity of almost all (96.9%) plants, and in cvs Fidelio, Woltario and Magnat of more than half of plants (80.1%, 66.9% and 55.5%, respectively), was positively confirmed by protein electrophoresis. The lowest proportion (36.1%) of off-type plants was recorded for cv. Lamberto. The proportion of off-type plants with unconfirmed identity was higher among the “higher plants” than among plants expressing the “earlier earing” or “no hairiness of neck” traits.
Źródło:
Biuletyn Instytutu Hodowli i Aklimatyzacji Roślin; 2009, 254; 21-39
0373-7837
2657-8913
Pojawia się w:
Biuletyn Instytutu Hodowli i Aklimatyzacji Roślin
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Study of Changes in Genetic Diversities and Hepatic Histological Structures of Two Ichthyofauna as a Consequence of Water Pollution in Two Different Areas of West Bengal
Autorzy:
Lahiri, Annesha
Das, Sohini
Datta, Rahul Kumar
Powiązania:
https://bibliotekanauki.pl/articles/1193627.pdf
Data publikacji:
2017
Wydawca:
Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
Tematy:
DNA gel electrophoresis
Labeo bata
Labeo rohita
Water pollution
fish liver
histopathology
ichthyofauna
physicochemical parameters
Opis:
Water pollution affects biodiversity harshly. An attempt was made to find out the effect of aquatic pollution on the tissues of the fish, taking two different types of carps from two different areas of West Bengal (Barrackpore in North 24 Parganas and Raiganj in North Dinajpur). The carps used were Labeo rohita (major carp) and Labeo bata (minor carp) and were sampled from two different ponds within 1 km of each locality. The physicochemical parameters of the respective ponds were estimated from which the fishes were collected and comparisons were made. Liver tissues were taken from the fishes for histological and molecular analysis. From histological studies, it was found that there were some irregularities in the liver tissue structures which might be due to water pollution. DNA isolation from fish liver followed by gel electrophoresis was done to check whether water pollution affect the integrity of the extracted genomic DNA of the fish samples.
Źródło:
World Scientific News; 2017, 71; 132-138
2392-2192
Pojawia się w:
World Scientific News
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Phaseolin seed variability in common bean [Phaseolus vulgaris L.] by capillary gel electrophoresis
Autorzy:
Salmanowicz, B P
Powiązania:
https://bibliotekanauki.pl/articles/2041989.pdf
Data publikacji:
2001
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
common bean
seed variability
phaseolin
capillary gel electrophoresis
storage protein
bean
Phaseolus vulgaris
genetic diversity
Źródło:
Journal of Applied Genetics; 2001, 42, 3; 269-281
1234-1983
Pojawia się w:
Journal of Applied Genetics
Dostawca treści:
Biblioteka Nauki
Artykuł

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