Informacja

Drogi użytkowniku, aplikacja do prawidłowego działania wymaga obsługi JavaScript. Proszę włącz obsługę JavaScript w Twojej przeglądarce.

English (EN)·Polski (PL)·Yкраїнський (UK)
Przejdź do strony domowej biblioteki Centralna Biblioteka Wojskowa Link otwiera się w nowym oknie Logo biblioteki Prolib Integro - strona głównaCentralna Biblioteka Wojskowa

Wyszukujesz frazę "electrophoresis" wg kryterium: Temat

  Lista filtrów
Wyrównaj
Wyświetlanie 1-25 z 95
Tytuł:
Bioactive coatings obtained by electrophoretic deposition on Ti15Mo alloy
Autorzy:
Szklarska, M.
Dercz, G.
Łosiewicz, B.
Stróż, D.
Powiązania:
https://bibliotekanauki.pl/articles/283998.pdf
Data publikacji:
2016
Wydawca:
Akademia Górniczo-Hutnicza im. Stanisława Staszica w Krakowie. Polskie Towarzystwo Biominerałów
Tematy:
biomaterials
electrophoresis
implants
Źródło:
Engineering of Biomaterials; 2016, 19, 138; 35
1429-7248
Pojawia się w:
Engineering of Biomaterials
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Application of electrophoretic methods for detection of protein-porphyrin complexes.
Autorzy:
Kowalska, Agnieszka
Kwiek, Piotr
Miłosz, Ewa
Gondek, Grzegorz
Romiszewska, Anna
Graczyk, Alfreda
Podhajska, Anna
Powiązania:
https://bibliotekanauki.pl/articles/1043407.pdf
Data publikacji:
2003
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
porphyrins
photodynamic therapy
PDT
electrophoresis
Opis:
Simple methods for detection and isolation of protein-porphyrin complexes were elaborated in our laboratory. They are based on the separation of protein-porphyrin complexes in native polyacrylamide gel and measurement of their fluorescence, with the use of two detection systems: the commercially available Gel DocTM 2000 system, and a system specially designed for the purpose of these investigations, concerning protein-porphyrin interactions. The fluorescent complexes can be electro-transferred from the gel onto PVDF membrane, eluted and analyzed in order to identify the protein interacting with porphyrins.
Źródło:
Acta Biochimica Polonica; 2003, 50, 4; 1155-1163
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Separation of low molecular rapeseed proteins by capillary electrophoresis
Autorzy:
Amarowicz, Ryszard
Panasiuk, Robert
Pari, Leelavinothan
Powiązania:
https://bibliotekanauki.pl/articles/2051054.pdf
Data publikacji:
2003
Wydawca:
Instytut Rozrodu Zwierząt i Badań Żywności Polskiej Akademii Nauk w Olsztynie
Tematy:
capillary electrophoresis
rapeseed
low molecular proteins
Opis:
Low molecular proteins were extracted and isolated from rapeseed. The main protein were characterised with molecular mass of 14 000 determined using capillary electrophoresis-SDS technique. UV spectrum showed that this protein appears as a complex with phenolic acids.
Źródło:
Polish Journal of Food and Nutrition Sciences; 2003, 53, 1s; 7-9
1230-0322
2083-6007
Pojawia się w:
Polish Journal of Food and Nutrition Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Genetic diversity of winter wheat cultivars and strains determined by electrophoregrams of gliadin and glutenin proteins
Autorzy:
Węgrzyn, Stanisław
Waga, Jacek
Powiązania:
https://bibliotekanauki.pl/articles/2198933.pdf
Data publikacji:
2004-06-20
Wydawca:
Instytut Hodowli i Aklimatyzacji Roślin
Tematy:
cluster analysis
electrophoresis
gliadins
glutenins
polymorphism
Opis:
Based on the polymorphism of gliadin and glutenin proteins relationships of 45 cultivars and strains of winter wheat were evaluated. The cluster analysis showed a considerable variation of the investigated genotypes. The similarity indices were calculated using the Nei and Li formula. The genetic distances between the cultivars ranged from 1.00 to 0.12. The highest similarity index - SI=1.00- being proof of the identical physicochemical composition of storage proteins, was found for the pair Farmer and Elena. The groups of similar and genetically distant cultivars have been presented in the form of a dendrogram. The possibility of using the results obtained from the cluster analysis in breeding programmes has been discussed.
Źródło:
Plant Breeding and Seed Science; 2004, 49; 51-61
1429-3862
2083-599X
Pojawia się w:
Plant Breeding and Seed Science
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
B-cell chronic lymphocytic leukemia-associated nuclear antigens
Autorzy:
Rogalińska, Małgorzata
Błoński, Jerzy Z
Robak, Tadeusz
Kiliańska, Zofia M
Powiązania:
https://bibliotekanauki.pl/articles/764933.pdf
Data publikacji:
2008
Wydawca:
Uniwersytet Łódzki. Wydawnictwo Uniwersytetu Łódzkiego
Tematy:
B-CLL specific antigens
electrophoresis
immunoblotting
Opis:
One- and two-dimensional polyacrylamide gel electrophoresis were used to compare the composition of nuclear polypeptides from normal and В-cell chronic lymphocytic leukemia mononuclear cells. Against two electrophoretically-specific nuclear proteins with molecular weight of 38/39 and 44/46 kD a from leukemic cells rabbit sera were obtained. As it was analyzed by Western blot technique the available antisera recognized the 38/39 kDa antigen in 53 of the 56 (94.6%), while the 44/46 kDa in 46 of the 49 (93.9%) of examined В-CLL nuclear fraction preparations, but not in normal ones. The pi values of described leukaemia-specific antigens were determined; p38/39 had pi in the range of pH 6.55 -7.00 and p44/46 - in the range of pH 6.2-6.4.
Źródło:
Acta Universitatis Lodziensis. Folia Biologica et Oecologica; 2008, 4
1730-2366
2083-8484
Pojawia się w:
Acta Universitatis Lodziensis. Folia Biologica et Oecologica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Poziom frakcji białkowych surowicy krwi u nurków
Levels of protein fractions in diver’s blood serum
Autorzy:
Dolatkowski, A.
Szczeblewski, B.
Lokucijewski, B.
Kudarenko, W.
Powiązania:
https://bibliotekanauki.pl/articles/1360608.pdf
Data publikacji:
2014
Wydawca:
Polskie Towarzystwo Medycyny i Techniki Hiperbarycznej
Tematy:
nurek
nurkowanie
białka krwi
elektroforeza białek
elektroforeza bibułowa
diver
diving
blood proteins
protein electrophoresis
paper electrophoresis
Opis:
Badano osocze krwi 72 nurków w wieku 21 do 23 lat nurków metodą elektroforezy bibułowej. Wartości frakcji białkowych w badaniu wyniosły: albuminy 61,7%, α1 globuliny 26%, α2 globuliny 6%, β globuliny 9,5% γ globuliny 20,2%. Wartości te mieściły się w normie fizjologicznej, ale największy procent odchyleń obserwowano w grupie I – nurków początkujących. Autorzy wiążą tę obserwację z możliwością działania czynnika emocjonalnego i chwilowymi zaburzeniami regulacji hormonalnej.
The study consisted in an analysis of the blood plasma of 72 divers aged between 21 and 23 years with paper electrophoresis method. The examined protein fractions reached the following values: albumins 61.7%, α1 globulins 26%, α2 globulins 6%, β globulins 9.5% γ globulins 20.2%. The obtained values were within physiological norm, with the most significant deviations observed in group I – beginner divers. The authors link this observation with a possible impact of an emotional factor and momentary disorders in hormonal regulation.
Źródło:
Polish Hyperbaric Research; 2014, 1(46); 93-106
1734-7009
2084-0535
Pojawia się w:
Polish Hyperbaric Research
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Experimental study on electrokinetic of kaolinite particles in aqueous suspensions
Autorzy:
Min, F.
Zhao, Q.
Liu, L.
Powiązania:
https://bibliotekanauki.pl/articles/110918.pdf
Data publikacji:
2013
Wydawca:
Politechnika Wrocławska. Oficyna Wydawnicza Politechniki Wrocławskiej
Tematy:
kaolinite
zeta potential
pH
immersion time
electrophoresis
Opis:
Influence of aqueous phase properties and process parameters on kaolinite particle zeta potential was quantified by electrophoresis experiments. The results indicated that pH strongly altered the zeta potential of kaolinite and it decreases at the beginning and then increases in the range of pH = 2–13. The activity of different cations changes the zeta potential and has the following tendency of Al3+ > Ca2+ > Mg2+ > Na+ and the zeta potential increases due to heterocoagulation of different mineral particles in suspension. It was found that the zeta potential of kaolinite particles increases after the suspension was stirred and decreases at the beginning, and then increases with soaking time. The FTIR results showed that the zeta potential takes into account ion adsorption and the change of Si–O, Al–O and Al–OH groups on the surface of the kaolinite particles.
Źródło:
Physicochemical Problems of Mineral Processing; 2013, 49, 2; 659-672
1643-1049
2084-4735
Pojawia się w:
Physicochemical Problems of Mineral Processing
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Native nucleic acid electrophoresis as an efficient alternative for genotyping method of influenza virus
Autorzy:
Pajak, Beata
Lepek, Krzysztof
Powiązania:
https://bibliotekanauki.pl/articles/1039250.pdf
Data publikacji:
2014
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
gel electrophoresis
SSCP
influenza
minor genetic variants
Opis:
Influenza viruses are the worldwide major causative agents of human and animal acute respiratory infections. Some of the influenza subtypes have caused epidemics and pandemics among humans. The varieties of methods are available for the rapid isolation and identification of influenza viruses in clinical and environmental samples. Since nucleic acids amplification techniques such as RT-PCR have been adapted, fast and sensitive influenza type and subtype determination is possible. However, in some ambiguous cases other, more detailed assay might be desired. The genetic material of influenza virus is highly unstable and constantly mutates. It is known that single nucleotide polymorphisms (SNPs) results in resistance to commercially available anti-viral drugs. The genetic drift of the virus could also result in weakening of immune response to infection. Finally, in a substantial number of patients co-infection with various virus strains or types has been confirmed. Although the detection of co-infection or presence of minor genetic variants within flu-infected patients is not a routine procedure, a rapid and wide spectrum diagnostics of influenza virus infections could reveal an accurate picture of the disease and more importantly, is crucial for choosing the appropriate therapeutics and virus monitoring. Herein we present the evidences that native gel electrophoresis and MSSCP - a method based on multitemperature single strand conformation polymorphism could furnish a useful technique for minor variants, which escape discovery by conventional diagnostic assays.
Źródło:
Acta Biochimica Polonica; 2014, 61, 3; 479-483
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Determination of glyphosate in water samples with the combination of cation-exchange chromatography and capillary electrophoresis
Autorzy:
Khrolenko, M.
Dżygiel, P.
Wieczorek, P.
Powiązania:
https://bibliotekanauki.pl/articles/347017.pdf
Data publikacji:
2003
Wydawca:
Politechnika Bydgoska im. Jana i Jędrzeja Śniadeckich. Wydział Technologii i Inżynierii Chemicznej
Tematy:
glyphosate
ion-exchange chromatography
capillary electrophoresis
pesticides
Opis:
An analytical method for determination of pesticide Glyphosate in water as a combination of cation-exchange chromatography and capillary electrophoresis is presented. Pure water was spiked with Glyphosate at concentrations 0.1, 0.25, 0.5 and 1 mM and percolated through a strong cation-exchange column packed with Dowex 50WX4-400 resin in its H+ form. The extract was further analyzed by capillary electrophoresis in indirect detection mode. The calibration curve for the pesticide in the range 0.1–2.5 mM was linear and with high degree of reproducibility. The obtained recoveries for all the studied concentrations amount 85%. Afterwards, the possibility to determine Glyphosate at the concentration 0.001mM (0.17 µg/ml) was checked by percolation of 100 ml of water sample through a column. The calculated recovery was 97.7%
Źródło:
Ars Separatoria Acta; 2003, 2; 56-63
1731-6340
Pojawia się w:
Ars Separatoria Acta
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Electrophoretic seed albumin patterns in the Vicia sativa L. aggregate
Autorzy:
Przybylska, J
Zimniak-Przybylska, Z.
Krajewski, P.
Powiązania:
https://bibliotekanauki.pl/articles/2043428.pdf
Data publikacji:
2000
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
polyacrylamide gel electrophoresis
genetic resource
chromosome number
Vicia sativa
variation
DNA polymorphism
vetch
seed albumin
electrophoresis
karyotype
isoenzyme variation
taxonomy
Źródło:
Journal of Applied Genetics; 2000, 41, 3; 139-149
1234-1983
Pojawia się w:
Journal of Applied Genetics
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Azotan MDMA (3,4-metylenodioksymetamfetaminy) – nietypowa postać soli czy nowy trend na rynku narkotyków?
MDMA (3,4-methylenedioxymethamphetamine) nitrate – an atypical salt form or a new trend in the drug market?
Autorzy:
Bachliński, Robert
Galarda, Małgorzata
Powiązania:
https://bibliotekanauki.pl/articles/499846.pdf
Data publikacji:
2017
Wydawca:
Centralne Laboratorium Kryminalistyczne Policji
Tematy:
MDMA
azotan
elektroforeza kapilarna
SEM
nitrate
capillary electrophoresis
Opis:
W niniejszym artykule przedstawiony został przypadek wystąpienia na nielegalnym rynku narkotykowym nietypowej postaci 3,4-metylenodioksymetamfetaminy (MDMA) w formie azotanu. Związek taki bardzo dobrze można zidentyfikować jedynie poprzez zastosowanie metod analitycznych, których nie wykorzystuje się rutynowo w badaniach kryminalistycznych tego typu substancji – elektroforezy kapilarnej (CE) czy skaningowej mikroskopii elektronowej (SEM). Należy więc być bardzo czujnym w przypadkach, gdy badania metodą chromatografii gazowej sprzężonej ze spektrometrią masową (GC-MS) jednoznacznie identyfikują badaną substancję, ale już badania w spektrofotometrii w podczerwieni tego nie potwierdzają.
The article presents a case involving an appearance of an atypical 3,4-methylenedioxymethamphetamine (MDMA) in the form of nitrate on the illicit drug market. This compound can be identified only by using such analytical methods as capillary electrophoresis (CE) or scanning electron microscopy (SEM), which are not routinely applied to forensic analyses of this type of substances. Therefore, particular caution should be exercised whenever a gas chromatography-mass spectrometry (GC-MS) method unambiguously identifies a substance, yet infrared spectroscopy fails to confirm this result.
Źródło:
Problemy Kryminalistyki; 2017, 296; 37-45
0552-2153
Pojawia się w:
Problemy Kryminalistyki
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Determination of lanthanum(III) and europium(III) – lactate interaction constants by capillary electrophoresis for environmental applications
Autorzy:
Bortolus, S.
Varenne, A.
Draye, M.
Gareil, P.
Czerwiński, K.
Cote, G.
Powiązania:
https://bibliotekanauki.pl/articles/347001.pdf
Data publikacji:
2003
Wydawca:
Politechnika Bydgoska im. Jana i Jędrzeja Śniadeckich. Wydział Technologii i Inżynierii Chemicznej
Tematy:
lanthanides(III)
lactate
separation
interaction constants
capillary electrophoresis
Opis:
In the present work, we investigated the ability of electrophoresis to determine the stability constant of the complex formed between lactate ions (a simple model of natural organic matter), and lanthanum (III) and europium (III), two model metal cations of actinides. The effect of lactate on lanthanide’s effective mobility is studied, and conditional metal ion-ligand interaction constants are determined on the basis of the variation in the effective mobilities (log βn = 2.23 for lanthanum (III) and 2.39 for europium (III), at 25°C and I=0.015 M). The originality of this contribution lies in the fact that we have chosen to detect the metal ion instead of the ligand by an indirect UV-visible detection and using a chromophoric compound (creatinine).
Źródło:
Ars Separatoria Acta; 2003, 2; 29-35
1731-6340
Pojawia się w:
Ars Separatoria Acta
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Genetic characterization of maize inbred lines and their mutual affinities.
Autorzy:
Bulińska-Radomska, Z.
Adamczyk, J.
Królikowski, Z.
Powiązania:
https://bibliotekanauki.pl/articles/2198825.pdf
Data publikacji:
2001-06-21
Wydawca:
Instytut Hodowli i Aklimatyzacji Roślin
Tematy:
Inbred lines
enzymes
electrophoresis
similarity
pedigree
heterosis
maize
Opis:
Twenty-five inbred lines of maize assigned to six heterotic groups and developed in Poland were studied electrophoretically to determine their purity and mutual relationships. The electrophoretic data were analyzed by principal component and cluster analyses. All lines (except five) were monomorphic in each enzyme studied. Electrophoretic affinities of inbred lines were, with some exception, were in accordance with the expectation inferred from their pedigrees. Lines of identical pedigree but with small differences in phenotypic or developmental characteristics had the lowest dissimilarity coefficient values and in cluster and principal component analyses behaved accordingly. They showed however diverse levels of clustering and dissimilarity coefficient values, which most likely reflected their departure from the common parental gene pool, which occurred in the course of breeding manipulations. Lines, which had one parent in common, were less similar to each other than those with identical pedigree. The most distinct electrophretically were lines with complex or dissimilar pedigree formulae as was confirmed by cluster and principal component analyses.
Źródło:
Plant Breeding and Seed Science; 2001, 45, 1; 11-19
1429-3862
2083-599X
Pojawia się w:
Plant Breeding and Seed Science
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Development and validation of a capillary electrophoresis method for determination of oxcarbazepine and zonisamide in pharmaceuticals
Autorzy:
Paw, B.
Wos, K.
Powiązania:
https://bibliotekanauki.pl/articles/3443.pdf
Data publikacji:
2008
Wydawca:
Instytut Medycyny Wsi
Tematy:
method development
validation
capillary electrophoresis
determination
oxcarbazepine
zonisamide
drug
Źródło:
Journal of Pre-Clinical and Clinical Research; 2008, 02, 2
1898-2395
Pojawia się w:
Journal of Pre-Clinical and Clinical Research
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Single-walled carbon nanotubes fractionation via electrophoresis
Autorzy:
Scheibe, B.
Lukaszczuk, P.
Rümmeli, M. H.
Kalenczuk, R. J.
Borowiak-Palen, E.
Powiązania:
https://bibliotekanauki.pl/articles/778838.pdf
Data publikacji:
2011
Wydawca:
Zachodniopomorski Uniwersytet Technologiczny w Szczecinie. Wydawnictwo Uczelniane ZUT w Szczecinie
Tematy:
SWCNTs
sole diazoniowe
frakcjonowanie
elektroforeza
diazonium salts
fractionation
electrophoresis
Opis:
This work presents the influence of the sonication time on the efficiency of the metallic/semiconducting (M/S) fractionation of diazonium salt functionalized single-walled carbon nanotubes (SWCNTs) via free solution electrophoresis (FSE) method. The SWCNTs synthesized via laser ablation were purified from amorphous carbon and catalyst particles through high vacuum annealing and subsequent refluxing processes in aqua regia solutions, respectively. The purified material was divided into two batches. The SWCNTs samples were dispersed in 1% SDS solution in ultrasound bath for 2 and 12 hours. Both dispersed SWCNTs samples were functionalized with p-aminobenzoic acid diazonium salt and fractionated via free solution electrophoresis method. Afterwards, the fractionated samples were recovered, purified from surfactant/functionalities by annealing and investigated via UV-Vis-NIR optical absorption spectroscopy (OAS). The efficiency of the fractionation process was estimated through the comparison of the van Hove singularities (vHS) presented in the obtained fractions to the starting SWCNTs.
Źródło:
Polish Journal of Chemical Technology; 2011, 13, 3; 1-4
1509-8117
1899-4741
Pojawia się w:
Polish Journal of Chemical Technology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Peripartum changes in serum activities of three major alkaline phosphatase isoenzymes in Holstein dairy cows
Autorzy:
Chiba, A.
Onomi, R.
Hatate, K.
Moriyama, T.
Goto, A.
Yamagishi, N.
Powiązania:
https://bibliotekanauki.pl/articles/2087301.pdf
Data publikacji:
2020
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
agarose gel electrophoresis (AGE)
alkaline phosphatase isoenzyme
periparturient cow
Źródło:
Polish Journal of Veterinary Sciences; 2020, 23, 3; 457-459
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Agarose gel electrophoresis pattern of serum alkaline phosphatase isoenzymes in Holstein cows during lactation
Autorzy:
Chiba, A.
Hatate, K.
Onomi, R.
Moriyama, T.
Goto, A.
Yamagishi, N.
Powiązania:
https://bibliotekanauki.pl/articles/2087309.pdf
Data publikacji:
2020
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
agarose gel electrophoresis (AGE)
alkaline phosphatase isoenzyme
lactating cow
Źródło:
Polish Journal of Veterinary Sciences; 2020, 23, 2; 317-319
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Chromatoelektroforeza aminokwasów
Khromatoehlektroforez aminokislot
Electrophoretic separation of amino-acids
Autorzy:
Podeszewski, Z.
Powiązania:
https://bibliotekanauki.pl/articles/874411.pdf
Data publikacji:
1962
Wydawca:
Narodowy Instytut Zdrowia Publicznego. Państwowy Zakład Higieny
Tematy:
elektroforeza
chromatografia
elektroforeza dwukierunkowa
elektrochromatografia
rozklad aminokwasow
aminokwasy
oznaczanie ilosciowe
electrophoresis
chromatography
bidirectional electrophoresis
electrochromatography
amino acid degradation
amino acid
quantitative determination
Źródło:
Roczniki Państwowego Zakładu Higieny; 1962, 13, 1
0035-7715
Pojawia się w:
Roczniki Państwowego Zakładu Higieny
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Isolation and characterization of zinc-binding proteins of canine seminal plasma
Autorzy:
Mogielnicka-Brzozowska, M.
Fraser, L.
Czarzasta, J.
Kordan, W.
Powiązania:
https://bibliotekanauki.pl/articles/32311.pdf
Data publikacji:
2012
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
isolation
zinc-binding protein
seminal plasma
dog
electrophoresis
reproductive process
Opis:
Zinc-binding proteins from seminal plasma (ZnBPs) originate in the secretions of different accessory sex glands and are implicated in key events associated with sperm-egg fertilization processes. This study describes the isolation and characterization of the ZnBPs of canine seminal plasma. Ejaculates were collected from three crossbred dogs for a 2-week period. The ZnBPs as well as non zinc-binding proteins (nZnBPs) were isolated by zinc-dependent affinity chromatography. The isolated fractions were subjected to native gel electrophoresis (one-dimensional polyacryamide gel electrophoresis, PAGE) and sodium dodecyl sulphate polyacryamide gel electrophoresis (SDS-PAGE), using denaturing and reducing conditions. Zinc-elution profile using affinity chromatography displayed two protein fractions represented by the nZnBPs and ZnBPs, respectively. Using native gel electrophoresis, it was found that both the nZnBPs and ZnBPs occurred in their native state as aggregates, ranging from 140 to 669 kDa. The nZnBPs were disaggregated into 8 protein bands, with molecular weights ranging from 10.7 to 79.7 kDa, following SDS-PAGE analysis. By contrast, SDS-PAGE analysis of the ZnBPs revealed 13 protein bands, with molecular weights ranging from 11.6 to 152.3 kDa. Densitometric analysis showed that 46-48% of nZnBPs could be accounted by protein fractions with molecular weights of 10.7 and 14.2 kDa. Also, 2 protein fractions with molecular weights of 11.6 and 14.3 kDa, were predominant in ZnBPs, accounting for approximately 28-30% of the total proteins. These results demonstrate the zinc-binding capacity of proteins secreted by the canine prostate. The findings of this study indicate that ZnBPs of canine seminal plasma comprise several protein fractions, which might be implicated in the reproductive processes in the dog.
Źródło:
Polish Journal of Veterinary Sciences; 2012, 15, 3
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Determination of plasma bone-specific alkaline phosphatase isoenzyme activity in Holstein calves using a commercial agarose gel electrophoresis kit
Autorzy:
Onomi, R.
Hatate, K.
Yamagishi, N.
Powiązania:
https://bibliotekanauki.pl/articles/2087395.pdf
Data publikacji:
2019
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
agarose gel electrophoresis (AGE)
bone-specific alkaline phosphatase isoenzyme
calf
Źródło:
Polish Journal of Veterinary Sciences; 2019, 4; 789-792
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Using capillary electrophoresis to study methylation effect on RNA-peptide interaction.
Autorzy:
Mucha, Piotr
Szyk, Agnieszka
Rekowski, Piotr
Agris, Paul
Powiązania:
https://bibliotekanauki.pl/articles/1043465.pdf
Data publikacji:
2003
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
methylated nucleosides
methylation
arginine methylation
RN-peptide interaction
capillary electrophoresis
Opis:
Methylation of RNA and proteins is one of a broad spectrum of post-transcriptional/translational mechanisms of gene expression regulation. Its functional signification is only beginning to be understood. A sensitive capillary electrophoresis mobility shift assay (CEMSA) for qualitative study of the methylation effect on biomolecules interaction is presented. Two RNA-peptide systems were chosen for the study. The first one consists of a 17-nucleotide analogue (+27-+43) of the yeast tRNAPhe anticodon stem and loop domain (ASLPhe) containing three of the five naturally occurring modifications (2'-O-methylcytidine (Cm32), 2'-O-methylguanine (Gm34) and 5-methylcytidine (m5C40)) (ASLPhe-Cm32,Gm34,m5C40) and a 15-amino-acid peptide (named tF2 : Ser1-Ile-Ser-Pro-Trp5-Gly-Phe-Ser-Gly-Leu10-Leu- Arg-Trp-Ser-Tyr15) selected from a random phage display library (RPL). A peptide-concentration-dependent formation of an RNA-peptide complex was clearly observable by CEMSA. In the presence of the peptide the capillary electrophoresis (CE) peak for triply methylated ASLPhe shifted from 18.16 to 20.90 min. Formation of the complex was not observed when an unmethylated version of ASLPhe was used. The second system studied consisted of the (+18)-(+44) fragment of the trans-activation response element of human immunodeficiency virus type 1 (TAR RNA HIV-1) and a 9-amino-acid peptide of the trans-activator of transcription protein (Tat HIV-1) Tat(49-57)-NH2 (named Tat1 : Arg49-Lys-Lys-Arg52-Arg-Gln-Arg-Arg- Arg57-NH2). In the presence of Tat(49-57)-NH2 a significant shift of migration time of TAR from 18.66 min to 20.12 min was observed. Methylation of a residue Arg52→Arg(Me)2, crucial for TAR binding, strongly disrupted formation of the complex. Only at a high micromolar peptide concentration a poorly shaped, broad peak of the complex was observed. CE was found to be an efficient and sensitive method for the analysis of methylation effects on interaction of biomolecules.
Źródło:
Acta Biochimica Polonica; 2003, 50, 3; 857-864
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Trypsin inhibitor electrophoretic patterns in Vicia faba L. and related species
Autorzy:
Zimniak-Przybylska, Z
Przybylska, J.
Krajewski, P.
Powiązania:
https://bibliotekanauki.pl/articles/2043622.pdf
Data publikacji:
1999
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
Vicia faba
trypsin inhibitor
Narbonensis
electrophoresis
faba bean
Bithynicae
taxonomy
Źródło:
Journal of Applied Genetics; 1999, 40, 4; 283-292
1234-1983
Pojawia się w:
Journal of Applied Genetics
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Sample Preparation for Determination of Biological Thiols by Liquid Chromatography and Electromigration Techniques
Autorzy:
Bald, Edward
Powiązania:
https://bibliotekanauki.pl/articles/896289.pdf
Data publikacji:
2004
Wydawca:
Uniwersytet Łódzki. Wydawnictwo Uniwersytetu Łódzkiego
Tematy:
sample preparation
liquid chromatografy
capillary electrophoresis
chemical derivatization
biological thiols
Opis:
Wydrukowano z dostarczonych Wydawnictwu UŁ gotowych materiałów
Majority of the bioanalytical or environmental methods do not use just one chromatografie or electrophoretic step, but rather involve several sample pretreatment steps which simplfy the matrix, and often preconcentrate and chemically modify the analytes. This work surveys typical procedures for sample preparation for most commonly analyzed biofluids with particular emphasis placed on chemical derivatization of sulfur amino acids for their determination by liquid phase separation techniques. Recent author's laboratory contribution to the development of sample preparation procedures is merked.
Źródło:
Acta Universitatis Lodziensis. Folia Chimica; 2004, 13
0208-6182
Pojawia się w:
Acta Universitatis Lodziensis. Folia Chimica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Proces impregnowania teflonem powłoki tlenkowej wytwarzanej metodą anodowego utleniania na podłożu aluminium
The Teflon impregnation of anodic coating onto aluminium substrate
Autorzy:
Kwolek, P.
Górecka, P.
Obłój, A.
Kwolek, U.
Dychtoń, K.
Drajewicz, M.
Sieniawski, J.
Powiązania:
https://bibliotekanauki.pl/articles/1208784.pdf
Data publikacji:
2016
Wydawca:
Stowarzyszenie Inżynierów i Techników Przemysłu Chemicznego. Zakład Wydawniczy CHEMPRESS-SITPChem
Tematy:
anodowanie
teflon
zużycie ścierne
korozja
elektroforeza
anodizing
abrasion resistance
corrosion
electrophoresis
Opis:
Powłoki tlenkowe wytworzone na podłożu aluminium o czystości technicznej impregnowano w procesie elektroforezy. Stosowano zawiesinę wodną cząstek teflonu. Ustalono wpływ wartości napięcia elektrycznego i czasu prowadzenia procesu elektroforezy na odporność na zużycie – w warunkach tarcia wytwarzanych impregnowanych powłok tlenkowych. Wykazano, że aluminium nieimpregnowane ma największą odporność na ścieranie. Proces impregnowania poprawia natomiast odporność korozyjną powłoki tlenkowej w roztworze HCl.
Electrophoresis was employed in order to impregnate anodic coatings obtained onto aluminium. The water suspension composed of teflon particles was applied. The influence of voltage and time of electrophoresis on the abrasion resistance of the impregnated coatings was determined. It was shown that anodized aluminium is the most wear resistant coating. However, the impregnation process enhances the corrosion resistance of anodic coatings in the HCl solution.
Źródło:
Chemik; 2016, 70, 7; 361-368
0009-2886
Pojawia się w:
Chemik
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Analysis of Genetic Similarities Between Clostridium Perfringens Isolates Isolated from Patients with Gas Gangrene and from Hospital Environment Conducted with the use of the PFGE Method
Autorzy:
Brzychczy-Włoch, Monika
Bulanda, Małgorzata
Powiązania:
https://bibliotekanauki.pl/articles/1395730.pdf
Data publikacji:
2014-03-01
Wydawca:
Index Copernicus International
Tematy:
Clostridium perfringens
gas gangrene
pulsed field gel electrophoresis - PFGE
epidemiological investigation
Opis:
The objective of the study was to perform a comparative analysis of genetic similarity, with the use of pulsed field gel electrophoresis (PFGE), of Clostridium perfringens isolates originating from patients with gas gangrene and from the hospital environment. The study encompassed two patients with a clinical and microbiological diagnosis of gas gangrene, who were hospitalized in one of the hospitals of the Małopolska province in the time period between 31st March 2012 and 18th May 2012. Clostridium perfringens isolates genotyping indicated that the isolates originating from the two studied patients did not display genetic similarity and represented two different PFGE types, which corresponded to two different clones (clone A and B). Whereas the strains isolated from the hospital environment were genetically identical with the strain coming from the second patient and represented one PFGE type, which corresponded to one clone (clone A). As a result of the study, it is possible to conclude that both patients developed endogenous infection. Even so, the examination of the hospital environment indicates the possibility of the appearance of exogenous infections. It prompts recommending and following the exact regulations of sanitary regime in the ward and the operating theater if a patient is diagnosed with gas gangrene.
Źródło:
Polish Journal of Surgery; 2014, 86, 3; 141-146
0032-373X
2299-2847
Pojawia się w:
Polish Journal of Surgery
Dostawca treści:
Biblioteka Nauki
Artykuł
Wyświetlanie 1-25 z 95

Ta witryna wykorzystuje pliki cookies do przechowywania informacji na Twoim komputerze. Pliki cookies stosujemy w celu świadczenia usług na najwyższym poziomie, w tym w sposób dostosowany do indywidualnych potrzeb. Korzystanie z witryny bez zmiany ustawień dotyczących cookies oznacza, że będą one zamieszczane w Twoim komputerze. W każdym momencie możesz dokonać zmiany ustawień dotyczących cookies