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    Wyświetlanie 1-39 z 39
    Tytuł:
    Wstępne badania w kierunku potwierdzenia obecności krwi ludzkiej w śladach biologicznych na podstawie analizy mRNA
    Preliminary examinations aiming at confirming presence of human blood in biological traces by means of mRNA analysis
    Autorzy:
    Gorzkiewicz, Marta
    Grabowska, Małgorzata
    Grzybowski, Tomasz
    Powiązania:
    https://bibliotekanauki.pl/articles/499775.pdf
    Data publikacji:
    2017
    Wydawca:
    Centralne Laboratorium Kryminalistyczne Policji
    Tematy:
    identyfikacja ludzkiej krwi
    profilowanie mRNA
    hemoglobina
    human blood identification
    mRNA profiling
    haemoglobin
    Opis:
    Celem projektu było stworzenie specyficznej i zarazem taniej metody służącej do wykrywania ludzkiej krwi w śladach biologicznych opartej na analizie mRNA hemoglobiny z wykorzystaniem reakcji PCR w czasie rzeczywistym, z zastosowaniem niespecyficznego detektora typu SYBR Green. Ostatecznie opracowany test umożliwia jednoczesną analizę krzywych topnienia trzech fragmentów o różnej długości – HBB61, HBA197 i HBB503, oraz dodatkowo genu referencyjnego – mRNA β-aktyny. Jednoznaczna identyfikacja możliwa była już dla 0,1 μl krwi. Metoda jest specyficzna tkankowo i gatunkowo. Analizowane markery mRNA cechują się większą stabilnością w porównaniu do białka hemoglobiny wykrywanego standardowymi metodami. Wynik profilowania mRNA wykazuje wartość predykcyjną odnośnie jakości materiału genetycznego i obecności mieszaniny płynów. Wyniki badań wykonanych w ramach projektu wskazują na potencjalną przydatność markerów HBB i HBA1 w rutynowych badaniach genetyczno-sądowych. Jednak niezbędne jest przeprowadzenie szerszego spektrum eksperymentów walidacyjnych, a szczególnie przeanalizowanie większej liczby rzeczywistych śladów biologicznych, dokładne określenie optymalnej ilości użytego RNA oraz sprawdzenie różnic osobniczych w poziomach ekspresji.
    The aim of the project was to develop a specific and at the same time economical method for detecting human blood in biological traces based on analysis of haemoglobin mRNA with use of PCR reaction in real time and non-specific SYBR Green detector. The test, which has eventually been developed enabled simultaneous analysis of melting curves for three fragments of various lengths: HBB61, HBA197 and HBB503, as well as an additional reference gene: mRNA β-actin. A definite identification was possible already for 0,1 μl of blood. The method is tissue and species specific. The analysed mRNA markers are characterized by high stability, as compared to haemoglobin detected by standard methods. The result of mRNA profiling shows the predictive value as regards quality of genetic material and occurrence of mixture of liquids. Results of analyses performed during the project indicate potential usefulness of HBB and HBA1 markers in routine forensic genetic examinations. However, it is necessary to carry out a broader spectrum of validation experiments, and particularly to analyse a larger number of actual biological casework and precisely determining an optimal quantity of RNA and identifying ontogenetic differences in the levels of expression.
    Źródło:
    Problemy Kryminalistyki; 2017, 295; 33-42
    0552-2153
    Pojawia się w:
    Problemy Kryminalistyki
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    The structure of fadL mRNA and its interactions with RybB sRNA
    Autorzy:
    Groszewska, Agata
    Wroblewska, Zuzanna
    Olejniczak, Mikolaj
    Powiązania:
    https://bibliotekanauki.pl/articles/1038749.pdf
    Data publikacji:
    2016
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    Hfq
    sRNA
    mRNA
    RybB
    fadL
    coding sequence
    Opis:
    Small bacterial RNAs (sRNAs) regulate translation by pairing with complementary sequences in their target mRNAs, in a process which is often dependent on the Hfq protein. Here, the secondary structure of a 95-nt long fragment of Salmonella fadL mRNA containing RybB sRNA binding site in the coding region was analyzed. The data indicated local rearrangements in this mRNA structure after the annealing of RybB. The filter retention data had shown that Hfq bound both RybB and the fadL mRNA fragment with tight affinities. Moreover, Hfq increased the rate of RybB annealing to fadL mRNA. These data indicate that Hfq directly participates in RybB interactions with the fadL mRNA.
    Źródło:
    Acta Biochimica Polonica; 2016, 63, 4; 835-840
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Metabolic enzymes that bind RNA: yet another level of cellular regulatory network?
    Autorzy:
    Cieśla, Joanna
    Powiązania:
    https://bibliotekanauki.pl/articles/1041266.pdf
    Data publikacji:
    2006
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    enzymes
    gene expression
    mRNA binding
    translation regulation
    Opis:
    Several enzymes that were originally characterized to have one defined function in intermediatory metabolism are now shown to participate in a number of other cellular processes. Multifunctional proteins may be crucial for building of the highly complex networks that maintain the function and structure in the eukaryotic cell possessing a relatively low number of protein-encoding genes. One facet of this phenomenon, on which I will focus in this review, is the interaction of metabolic enzymes with RNA. The list of such enzymes known to be associated with RNA is constantly expanding, but the most intriguing question remains unanswered: are the metabolic enzyme-RNA interactions relevant in the regulation of cell metabolism? It has been proposed that metabolic RNA-binding enzymes participate in general regulatory circuits linking a metabolic function to a regulatory mechanism, similar to the situation of the metabolic enzyme aconitase, which also functions as iron-responsive RNA-binding regulatory element. However, some authors have cautioned that some of such enzymes may merely represent "molecular fossils" of the transition from an RNA to a protein world and that the RNA-binding properties may not have a functional significance. Here I will describe enzymes that have been shown to interact with RNA (in several cases a newly discovered RNA-binding protein has been identified as a well-known metabolic enzyme) and particularly point out those whose ability to interact with RNA seems to have a proven physiological significance. I will also try to depict the molecular switch between an enzyme's metabolic and regulatory functions in cases where such a mechanism has been elucidated. For most of these enzymes relations between their enzymatic functions and RNA metabolism are unclear or seem not to exist. All these enzymes are ancient, as judged by their wide distribution, and participate in fundamental biochemical pathways.
    Źródło:
    Acta Biochimica Polonica; 2006, 53, 1; 11-32
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Influence of reagent formulation on mRNA quantification by RT-PCR using imported external standard curves
    Autorzy:
    Farriol, Mireia
    Orta, Xavi
    Powiązania:
    https://bibliotekanauki.pl/articles/1041328.pdf
    Data publikacji:
    2005
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    mRNA quantification
    ornithine decarboxylase
    RT-PCR
    Opis:
    Use of an imported external standard curve is common in real-time quantitative RT-PCR. Two practical strategies for long-term experiments include importing a grand mean standard curve to all accumulated runs or using daily imported standard curves, fixing the slope at the beginning of the experiment and calibrating successive runs with curves generated from this imported slope, adding a single standard that registers the variation in the y-intercept. This study determines the influence that a change in reagent lots has on these two calibration approaches when determining mRNA copy numbers of the ornithine decarboxylase and porphobilinogen deaminase genes. Two sets of determinations were run with the use of lot A and lot B. A marked decrease in the crossing points (Cp) in the standards for both genes at all concentration levels was observed with the change in lots. A grand mean standard curve was generated for each gene and each set and comparisons between the sets were performed. Statistically significant differences were found with respect to the y-intercept but not the slope, suggesting that the change of reagent lot affected the detection sensitivity but not the efficiency of the reaction. The excellent correlation coefficients obtained for these curves for each gene were not achieved when overall data from both sets were combined to generate an overall grand mean standard curve. We conclude that when faced with a change of RT-PCR reagent lot that will affect the detection sensitivity of the method, samples should be calculated with either the daily imported standard curves or with the respective grand mean standard curve for each lot.
    Źródło:
    Acta Biochimica Polonica; 2005, 52, 4; 845-848
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    The nuclear cap-binding protein complex is not essential for nonsense-mediated mRNA decay (NMD) in plants
    Autorzy:
    Dzikiewicz-Krawczyk, Agnieszka
    Piontek, Paulina
    Szweykowska-Kulińska, Zofia
    Jarmołowski, Artur
    Powiązania:
    https://bibliotekanauki.pl/articles/1040693.pdf
    Data publikacji:
    2008
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    nuclear cap-binding protein complex
    alternative splicing
    mRNA surveillance
    NMD
    premature terminatin codon
    nonsense-mediated mRNA decay
    Opis:
    In this study we investigated whether in plants, like in mammals, components of the nuclear cap-binding protein complex (CBC) are involved in nonsense-mediated mRNA decay (NMD). We selected several genes producing at least two alternatively spliced mRNA variants: one with a premature termination codon (PTC+) and another without it (PTC-). For each gene the PTC+/PTC- ratio was calculated using RT-PCR and direct sequencing in four Arabidopsis thaliana lines: wild type, the NMD mutant atupf3-1 and two CBC mutants: cbp20 and abh1. Whereas in the NMD mutant the ratios of PTC+/PTC- splice variants were higher than in wild-type plants, the two CBC mutants investigated showed no change in the PTC+/PTC- ratios. Our results suggest that neither CBP20 nor CBP80 is involved in NMD in A. thaliana.
    Źródło:
    Acta Biochimica Polonica; 2008, 55, 4; 825-828
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Licensed liposomal vaccines and adjuvants in the antigen delivery system
    Autorzy:
    Krasnopolsky, Yuriy
    Pylypenko, Daria
    Powiązania:
    https://bibliotekanauki.pl/articles/16648151.pdf
    Data publikacji:
    2022
    Wydawca:
    Polska Akademia Nauk. Czasopisma i Monografie PAN
    Tematy:
    vaccine
    mRNA
    liposomes
    adjuvant
    virosoma
    lipid nanoparticles
    Opis:
    Liposomes (LSs) are promising nanoparticles with unique properties such as controlled nanosize, large surface area, increased reactivity, and ability to undergo modification. Worldwide, licensed liposomal forms of antibiotics, hormones, antioxidants, cytostatics, ophthalmic drugs, etc., are available on the pharmaceutical market. This review focuses on the adjuvant properties of LSs in the production of vaccines (VACs). LS-VACs have the following advantages: antigens with low immunogenicity can become highly immunogenic; LSs can include both hydrophilic and hydrophobic antigens; LSs allow to achieve a prolonged specific action of antibodies; and LSs reduce the toxicity and pyrogenicity of encapsulated antigens and adjuvants. The immune response is influenced by the composition of the liposomal membrane, physicochemical characteristics of lipids, antigen localization in LSs, interaction of LSs with complement, and a number of proteins, which leads to opsonization. The major requirements for adjuvants are their ability to enhance the immune response, biodegradability, and elimination from the organism, and LSs fully meet these requirements. The effectiveness and safety of LSs as carriers in the antigen delivery system have been proven by the long-term clinical use of licensed vaccines against hepatitis A, influenza, herpes zoster, malaria, and COVID-19.
    Źródło:
    BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2022, 103, 4; 409-423
    0860-7796
    Pojawia się w:
    BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    The role of the 5 terminal region of p53 mRNA in the p53 gene expression
    Autorzy:
    Swiatkowska, Agata
    Zydowicz, Paulina
    Sroka, Joanna
    Ciesiołka, Jerzy
    Powiązania:
    https://bibliotekanauki.pl/articles/1038716.pdf
    Data publikacji:
    2016
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    p53
    transcription
    translation
    non-coding region
    mRNA
    IRES
    Opis:
    The p53 tumour suppressor protein is one of the major factors responsible for cell cycle regulation and protection against cancer development. This is why it is often referred to as "the guardian of the genome". On the other hand, mutations in the p53 gene are connected with more than 50% of tumours of various types. The thirty-six years of extensive research on the p53 gene and its protein products have shown how sophisticated the p53-based cell system control is. An additional level of complexity of the p53 research is connected with at least twelve p53 isoforms which have been identified in the cell. Importantly, disturbance of the p53 isoforms' expression seems to play a key role in tumorigenesis, cell differentiation and cell response to pathogenic bacteria, and RNA and DNA viruses. Expression of various p53 isoforms results from the usage of different transcription promoters, alternative splicing events and translation initiation from alternative AUG codons. The importance of the 5'-terminal regions of different p53 mRNA transcripts in the multi-level regulation of the p53 gene has recently been documented. In this review we focus on the structural features of these regions and their specific role in the p53 translation initiation process.
    Źródło:
    Acta Biochimica Polonica; 2016, 63, 4; 645-651
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Differential stability of mitochondrial mRNA in HeLa cells
    Autorzy:
    Piechota, Janusz
    Tomecki, Rafał
    Gewartowski, Kamil
    Szczęsny, Roman
    Dmochowska, Aleksandra
    Kudła, Marek
    Dybczyńska, Lien
    Stepien, Piotr
    Bartnik, Ewa
    Powiązania:
    https://bibliotekanauki.pl/articles/1041282.pdf
    Data publikacji:
    2006
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    polyadenylation
    HeLa
    mitochondria
    poly(A) polymerase
    mRNA turnover
    Opis:
    The physiological significance and metabolism of oligoadenylated and polyadenylated human mitochondrial mRNAs are not known to date. After study of eight mitochondrial transcripts (ND1, ND2, ND3, ND5, CO1, CO2, ATP6/8 and Cyt. b) we found a direct correlation between the half-lives of mitochondrial mRNAs and their steady-state levels. Investigation of the mt-mRNA decay after thiamphenicol treatment indicated that three transcripts (ND2, ND3 and Cyt. b) are significantly stabilized after inhibition of mitochondrial translation. Careful analysis one of them, ND3, showed that inaccurate processing of the H-strand RNA precursor may occasionally occur between the ND3 and tRNAArg locus leading to synthesis of ND3 mRNAs lacking the STOP codon. However, analysis of the oligo(A) fraction observed in case of the ND3 indicates that partially polyadenylated mRNAs are linked rather to the transcription process than to the translation-dependent deadenylation. Analysis of ND3 mRNA turnover in cells with siRNA-mediated knock-down of the mitochondrial poly(A) polymerase shows that strongly decreased polyadenylation does not markedly affect the decay of this transcript. We present a model where oligoadenylated mitochondrial transcripts are precursors of molecules containing full length poly(A) tails.
    Źródło:
    Acta Biochimica Polonica; 2006, 53, 1; 157-168
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Contributions of the Hfq protein to translation regulation by small noncoding RNAs binding to the mRNA coding sequence
    Autorzy:
    Wroblewska, Zuzanna
    Olejniczak, Mikolaj
    Powiązania:
    https://bibliotekanauki.pl/articles/1038726.pdf
    Data publikacji:
    2016
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    Hfq
    sRNA
    mRNA
    coding sequence
    RNase E
    ribosome
    Opis:
    The bacterial Sm-like protein Hfq affects the regulation of translation by small noncoding RNAs (sRNAs). In this way, Hfq participates in the cell adaptation to environmental stress, regulation of cellular metabolism, and bacterial virulence. The majority of known sRNAs bind complementary sequences in the 5'-untranslated mRNA regions. However, recent studies have shown that sRNAs can also target the mRNA coding sequence, even far downstream of the AUG start codon. In this review, we discuss how Hfq contributes to the translation regulation by those sRNAs which bind to the mRNA coding sequence.
    Źródło:
    Acta Biochimica Polonica; 2016, 63, 4; 701-707
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    COVID-19: szczepienie mRNA dorosłych i dzieci. Niskie ryzyko zapalenia mięśnia sercowego
    Autorzy:
    Krzystyniak, Krzysztof
    Powiązania:
    https://bibliotekanauki.pl/articles/2037195.pdf
    Data publikacji:
    2022-02-16
    Wydawca:
    Wydawnictwo Naukowe Medyk sp. z o.o.
    Tematy:
    COVID-19
    szczepionki mRNA
    zapalenie mięśnia sercowego
    zapalenie osierdzia
    zespół ostrej niewydolności oddechowej (ARDS)
    mRNA vaccines
    myocarditis
    pericarditis
    acute respiratory distress syndrome (ARDS)
    Opis:
    Aktualnie ocenia się, że bez szczepień prawdopodobnie prawie wszyscy — w tym małe dzieci — w pewnym momencie ich życia zostaną zarażeni koronawirusem SARS-CoV-2. Zaleca się szczepienie przeciw zakażeniom COVID-19 szczepionkami z informacyjnym mRNA dla dorosłych i dla dzieci w wieku 5–11 lat. Wielu rodziców jest niechętnych lub sprzeciwia się zapewnieniu dzieciom tej ochrony. Istnieje niskie ryzyko zapalenia mięśnia sercowego, około 5 przypadków na milion osób otrzymujących szczepionki mRNA anty-COVID-19. Jednak podjęcie negatywnej decyzji o zaszczepieniu nie jest wyborem pozbawionym ryzyka, a raczej jest to wybór poważniejszego ryzyka.
    It is now estimated, that without vaccination, it is likely that almost everyone-including young children – will be infected with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) at some point in their lives. Vaccination with messenger RNA (mRNA) anti-COVID-19 vaccine is recommended for adults and for children between 5 and 11 years of age. Many parents are reluctant or opposed to seeking this protection for children. Low risk of myocarditis has been reported, approximately 5 cases per 1,000,000 individuals receiving mRNA COVID-19 vaccines, possibly as high as 1 per 10,000 in young men. However, a choice not to get a vaccine is not a risk-free choice; rather, it’s a choice to take a different and more serious risk.
    Źródło:
    Gabinet Prywatny; 2022, 280, 01; 5-8
    2353-8600
    Pojawia się w:
    Gabinet Prywatny
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Histopathological characteristics of myocarditis following COVID-19 vaccination: a scoping review
    Autorzy:
    Kalali, Datis
    Anestakis, Doxakis
    Powiązania:
    https://bibliotekanauki.pl/articles/24987723.pdf
    Data publikacji:
    2023-10-09
    Wydawca:
    Gdański Uniwersytet Medyczny
    Tematy:
    histopathology
    myocarditis
    myocardial inflammation
    COVID-19 vaccine
    mRNA vaccines
    Opis:
    Introduction: Cases of myocarditis in people who were vaccinated against COVID-19 have been reported in the recent years. Nevertheless, the histopathological features and the pathomechanisms in these cases are still unclear. Hence, a scoping review of existing literature was performed to discover the histopathological features of myocarditis induced by the above-mentioned vaccine. Material and Methods: A search was performed in the PubMed, Scopus and EMBASE databases to retrieve the relevant records, involving analyses of biopsy and autopsy specimens. Baseline characteristics of the patients and the histopathological characteristics of the respective specimens were extracted and recorded. Results: Overall, 24 case reports and case series (involving a total of 54 patients) were included in this scoping review. The following signs of inflammation were present in the specimens: lymphocyte infiltration (64.8%), eosinophilic infiltration (29.6%), neutrophil infiltration (3.7%) and giant-cell formation (1.9%). Other features included myocardial tissue necrosis (20.4%), the presence of the SARS-CoV-2 spike protein (16.7%) and microthrombosis (3.7%). Conclusions: The histopathological characteristics of SARS-CoV-2 vaccine-induced myocarditis were heterogenous, the only common characteristic was the presence of lymphocyte infiltration in more than half of the cases. Studies of unreported past cases may provide further insights into the topic.
    Źródło:
    European Journal of Translational and Clinical Medicine; 2023, 6, 2; 85-90
    2657-3148
    2657-3156
    Pojawia się w:
    European Journal of Translational and Clinical Medicine
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Antibody response after SARS-CoV-2 mRNA vaccine in naïve and previously infected healthcare workers
    Odpowiedź na przeciwciała po szczepionce mRNA SARS-CoV-2 u uprzednio niezainfekowanych oraz zainfekowanych pracowników medycznych
    Autorzy:
    Huțanu, Adina
    Dobreanu, Minodora
    Powiązania:
    https://bibliotekanauki.pl/articles/2056546.pdf
    Data publikacji:
    2022
    Wydawca:
    Akademia Bialska Nauk Stosowanych im. Jana Pawła II w Białej Podlaskiej
    Tematy:
    COVID-19 antibody testing
    mRNA vaccine
    immune response
    COVID-19
    healthcare workers
    testowanie przeciwciał COVID-19
    szczepionka mRNA
    odpowiedź immunologiczna
    pracownicy służby zdrowia
    Opis:
    Background. Evaluating and monitoring plasma levels of anti-SARS-CoV-2 antibodies in healthcare workers, together with the vaccination of this at-risk population is important for maintaining the viability of the healthcare system, especially during the emergence of new viral variants. The aim of this study is to investigate plasma levels of anti-SARS-CoV-2 antibodies in healthcare providers following full vaccination, in both naïve and previously infected individuals. Material and methods. Complete data was available for 89 healthcare workers from the larger group of 102 initial participants. Plasma was collected at least one month, and no later than two months after the full dose of an mRNA vaccine, and analyzed by determining the total antibody concentration against the spike protein using an ECLIA kit. Results. The degree of humoral-specific immune response was at least 5-fold higher in previously infected healthcare workers compared to naïve persons that received the vaccine only. The highest titer was found in office-based staff, relative to those found in doctors and nurses. However, this difference lacks statistical significance. Among previously infected participants, nurses had significantly higher antibody titers, when compared to doctors. Conclusions. The study revealed a sustained immune response after mRNA vaccine among healthcare workers, with enhanced response in previously infected subjects, highlighting a boosting effect of the vaccine.
    Wprowadzenie. Ocena i monitorowanie stężenia przeciwciał anty-SARS-CoV-2 w osoczu pracowników służby zdrowia oraz szczepienia tej szczególnej grupy ryzyka są ważne do utrzymania sprawności systemu opieki zdrowotnej, zwłaszcza w przypadku pojawienia się nowych wariantów wirusa. Celem pracy jest zbadanie poziomu przeciwciał anty-SARS-CoV-2 w osoczu pracowników służby zdrowia po pełnym szczepieniu, zarówno u osób wcześniej niezainfekowanych, jak i zainfekowanych. Materiał i metody. Kompletne dane były dostępne dla 89 pracowników służby zdrowia pochodzących z większej, wstępnej grupy 102 uczestników. Osocze pobierano co najmniej 1 miesiąc, a nie później niż 2 miesiące po podaniu pełnej dawki szczepionki mRNA i analizowano poprzez określenie całkowitego stężenia przeciwciał skierowanych przeciwko białku S przy użyciu zestawu ECLIA. Wyniki. Stopień humoralnej odpowiedzi swoistej był co najmniej 5-krotnie wyższy u uprzednio zakażonych pracowników służby zdrowia w porównaniu z osobami wcześniej niezainfekowanymi, które otrzymały tylko szczepionkę. Wyższe miano stwierdzono wśród osób pracujących w biurze niż u lekarzy i pielęgniarek, jednak różnica ta nie jest istotna statystycznie. Wśród wcześniej zakażonych uczestników pielęgniarki miały znacząco wyższe miana przeciwciał w porównaniu z lekarzami. Wnioski. Badanie wykazało trwałą odpowiedź immunologiczną po podaniu szczepionki mRNA wśród pracowników służby zdrowia, a także zwiększoną odpowiedź u osób wcześniej zakażonych, co podkreśla wzmacniający efekt szczepionki.
    Źródło:
    Health Problems of Civilization; 2022, 16, 1; 48-56
    2353-6942
    2354-0265
    Pojawia się w:
    Health Problems of Civilization
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Cyclopenta[c]phenanthrene induction of CYP1A in brain of rainbow trout (Oncorhynchus mykiss)
    Autorzy:
    Brzuzan, P.
    Woźny, M.
    Łuczyński, M. K.
    Góra, M.
    Ciesielski, S.
    Kuźmiński, H.
    Powiązania:
    https://bibliotekanauki.pl/articles/363272.pdf
    Data publikacji:
    2007
    Wydawca:
    Uniwersytet Warmińsko-Mazurski w Olsztynie
    Tematy:
    benzo(a)piren
    mózg
    CYP1A
    cyklopenta[c]fenantren
    absolutna kwantyfikacja mRNA
    pstrąg tęczowy
    benzo(a)pyrene
    brain
    cyclopenta[c]phenanthrene
    mRNA absolute quantification
    rainbow trout
    Opis:
    We assessed the effects of cyclopenta[c]phenanthrene (CP[c]Ph) and benzo[a]pyrene (B[a]P; positive control) on CYP1A gene expression in brain of juvenile rainbow trout (Oncorhynchus mykiss) using the quantitative reverse transcription polymerase chain reaction (Q-RT-PCR). A group of hatchery raised rainbow trout, with an average body mass of 49.4 g and total length of 15.5 cm were given an intraperitoneal injection (10 mg*kg-1) of either CP[c]Ph or B[a]P in corn oil (2 mg*mi-1 corn oil) or corn oil alone (control). After 24 and 48 h, trout brains were collected for mRNA isolation and analysis. After 24 hours of the exposure, only B[a]P-treated rainbow trout had 10-fold higher number of CYP1A transcripts (mean = 3.63*106 transcripts*µg-1 total RNA) than control fish (3.24*105 transcripts*µg-1 total RNA; Tukey test, P<0.05). After 48 hrs, significantly higher levels of CYP1A expression (Tukey test, P<0.001) were found in either CP[c]Ph- or B[a]P- induced group (1.45*106 and 6.92*106 transcriptsźµg-1 total RNA, respectively) over a control group (mean=1.41*105 transcripts*µg-1 total RNA). The finding that CYP1A in brain tissue was inducible by CP[c]Ph, a polycyclic aromatic hydrocarbon (PAH) of different than B[a]P planar characteristics, may further validate the use of rainbow trout brain CYP1A mRNA levels as a biomarker of PAH exposure.
    Źródło:
    Environmental Biotechnology; 2007, 3, 1; 10-14
    1734-4964
    Pojawia się w:
    Environmental Biotechnology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Analysis of integrins and vascular endothelial growth factor isoforms mRNA expression in the canine uterus during perimplantation period
    Autorzy:
    Bukowska, D.
    Kempisty, B.
    Jackowska, M.
    Wozna, M.
    Antosik, P.
    Piotrowska, H.
    Jaskowski, J.M.
    Powiązania:
    https://bibliotekanauki.pl/articles/31799.pdf
    Data publikacji:
    2011
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    integrin
    vascular endothelial growth factor
    isoform
    mRNA expression
    dog
    uterus
    pregnancy
    bitch
    Opis:
    Integrins are the major receptors within the extracellular matrix (ECM) that mediate several functions connected with cell life and metabolism, such as cell adhesion, migration, cytoskeletal organization, proliferation, survival, and differentiation. A vascular endothelial growth factor (VEGF) is one of the most important angiogenic factors. It has been suggested that the expression of this gene may play crucial physiological roles in reproductive organs. All investigated endometrial tissues were isolated on day 10-12 after mating. Control bitches, used in this study, were in metestrus, which was determined according to the vaginal cytology and progesterone level in blood. Early pregnancy was verified by flushing the uterine horns with PBS. Total RNA was isolated from the bitches endometrium by means of the Chomczyński and Sacchi method, treated by DNase I, and reverse-transcribed into cDNA. A quantitative analysis of integrins α2b, β2 and β3, VEGF 164, 182 and 188 cDNA was performed by RT-PCR. In results we have shown an increased expression of all investigated genes (integrins α2b, β2 and β3, VEGF 164, 182, and 188) in pregnant bitches uterus as compared to non-pregnant females (P<0.001). Our results indicated that the expression of genes encoding integrins and vascular endothelial growth factors is different in relation to the time of the embryo implantation and it is increased in the first period of this process. This may be associated with the induction of specific mechanisms responsible for receptivity of uterus following the embryo attachment. In addition, all of investigated genes are up-regulated in a pregnancy-specific manner and the increased expression of these genes may regulate the uterus function during the implantation of canine embryos.
    Źródło:
    Polish Journal of Veterinary Sciences; 2011, 14, 2
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Interaction of three Caenorhabditis elegans isoforms of translation initiation factor eIF4E with mono- and trimethylated mRNA 5 cap analogues.
    Autorzy:
    Stachelska, Alicja
    Wieczorek, Zbigniew
    Ruszczyńska, Katarzyna
    Stolarski, Ryszard
    Pietrzak, Monika
    Lamphear, Barry
    Rhoads, Robert
    Darżynkiewicz, Edward
    Jankowska-Anyszka, Marzena
    Powiązania:
    https://bibliotekanauki.pl/articles/1043732.pdf
    Data publikacji:
    2002
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    fluorescence quenching
    eIF4E
    Caenorhabditis elegans
    mRNA 5' cap
    translation initiation
    Opis:
    Translation initiation factor eIF4E binds the m7G cap of eukaryotic mRNAs and mediates recruitment of mRNA to the ribosome during cap-dependent translation initiation. This event is the rate-limiting step of translation and a major target for translational control. In the nematode Caenorhabditis elegans, about 70% of genes express mRNAs with an unusual cap structure containing m32,2,7G, which is poorly recognized by mammalian eIF4E. C. elegans expresses five isoforms of eIF4E (IFE-1, IFE-2, etc.). Three of these (IFE-3, IFE-4 and IFE-5) were investigated by means of spectroscopy and structural modelling based on mouse eIF4E bound to m7GDP. Intrinsic fluorescence quenching of Trp residues in the IFEs by iodide ions indicated structural differences between the apo and m7G cap bound proteins. Fluorescence quenching by selected cap analogues showed that only IFE-5 forms specific complexes with both m7G- and m32,2,7G-containing caps (Kas 2×106 M-1 to 7×106 M-1) whereas IFE-3 and IFE-4 discriminated strongly in favor of m7G-containing caps. These spectroscopic results quantitatively confirm earlier qualitative data derived from affinity chromatography. The dependence of Kas on pH indicated optimal cap binding of IFE-3, IFE-4 and IFE-5 at pH 7.2, lower by 0.4 pH units than that of eIF4E from human erythrocytes. These results provide insight into the molecular mechanism of recognition of structurally different caps by the highly homologous IFEs.
    Źródło:
    Acta Biochimica Polonica; 2002, 49, 3; 671-682
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Localization of the calreticulin gene mRNA in unpollinated and pollinated styles of Petunia hybrida Hort.
    Autorzy:
    Lenartowska, M
    Lenartowski, R.
    Bednarska, E.
    Powiązania:
    https://bibliotekanauki.pl/articles/2041944.pdf
    Data publikacji:
    2001
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    pistil
    Petunia hybrida
    gene
    pollen tube
    transcription
    mRNA
    protein
    calreticulin
    transmitting cell
    Źródło:
    Journal of Applied Genetics; 2001, 42, 1; 15-20
    1234-1983
    Pojawia się w:
    Journal of Applied Genetics
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Evidence for differential effects of glucose and cycloheximide on mRNA levels of peroxisome proliferator-activated receptor- (PPAR-) machinery members: Superinduction of PPAR-γ1 and -γ2 mRNAs
    Autorzy:
    Rypka, Miroslav
    Veselý, Jaroslav
    Powiązania:
    https://bibliotekanauki.pl/articles/1040405.pdf
    Data publikacji:
    2010
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    peroxisome proliferator-activated receptor
    HepG2 cells
    mRNA stability
    PPAR-γ coactivator
    superinduction
    Opis:
    Quantitative real-time RT-PCR study was conducted to reveal the effects of normal (5 mmol/l) and high (30 mmol/l) glucose without or with oleate (0.3 mmol/l) on mRNA levels of peroxisome proliferator-activated receptor- (PPAR-)α, -γ1, -γ2, and peroxisome proliferator-activated receptor-γ coactivator- (PGC-)1α and -1β in commercial human hepatoma-derived HepG2 cells maintained under low-serum condition. Significant decrease in PPAR-γ1 and PGC-1α mRNA levels to about 50 % was observed during the first 4 h incubation period. During the next 4 h period, both PPAR-γ1 and PGC-1α mRNAs were partly but significantly restored in high glucose batches. In this period, the presence of the transcriptional inhibitor actinomycin D revealed a significant protective effect of excess glucose on mature PPAR-γ1 and PGC-1α mRNAs. Furthermore, PPAR-γ1 and -γ2 mRNAs were differentially superinduced 1.2-2.5 fold in cells upon the administration of the translational inhibitor cycloheximide. When the cells were co-treated with the combination of cycloheximide and actinomycin D, superinduction was completely suppressed, however. Altogether, the experiments revealed, first, an unexpected protective effect of abundant glucose on PPAR-γ1 and PGC-1α mRNAs in HepG2 cells. Second, we demonstrated cycloheximide-induced, transcription-dependent upregulation of mature PPAR-γ1 and -γ2 mRNAs in HepG2 cells associated with preferential expression of the PPAR-γ2 mRNA variant. The results draw attention to as yet unexplored mechanisms involved in the control of PPAR and PGC genes.
    Źródło:
    Acta Biochimica Polonica; 2010, 57, 2; 209-215
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    The construction of recombinant vectors for a DNA vaccination of rats against fasciolosis
    Autorzy:
    Kofta, W.
    Wedrychowicz, H.
    Powiązania:
    https://bibliotekanauki.pl/articles/838715.pdf
    Data publikacji:
    1998
    Wydawca:
    Polskie Towarzystwo Parazytologiczne
    Tematy:
    parasite
    vaccination
    cattle
    economic loss
    fluke
    fasciolosis
    mRNA
    Fasciola hepatica
    liver
    cysteine proteinase
    cDNA
    rat
    Źródło:
    Annals of Parasitology; 1998, 44, 3
    0043-5163
    Pojawia się w:
    Annals of Parasitology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Characterization and expression analysis of the yellow lupin (Lupinus luteus L.) gene coding for nodule specific proline-rich protein.
    Autorzy:
    Karłowski, Wojciech
    Stróżycki, Paweł
    Legocki, Andrzej
    Powiązania:
    https://bibliotekanauki.pl/articles/1044363.pdf
    Data publikacji:
    2000
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    symbiotic nitrogen fixation
    plant gene expression
    ENOD2
    proline-rich proteins
    early nodulins
    mRNA degradation
    Opis:
    The LlPRP2 gene coding for a proline-rich protein shows a high level of similarity to, as well as significant differences from the family of ENOD2 nodule-specific genes. Several sequence motifs with putative regulatory function were identified in the 5' and 3' noncoding regions of the LlPRP2 gene. Northern blot analysis revealed that the expression of the LlPRP2 gene begins 9 days after inoculation of yellow lupin roots with Bradyrhizobium sp. (Lupinus); the expression is restricted to symbiotic nodules and is not detected in other tissues or organs. Detailed hybridization analysis showed that, when expression is activated, the LlPRP2 transcript is modified so as to produce at least three bands and a continuous distribution of decay intermediates. The modification of the LlPRP2 transcript probably involves degradation from the 5'- and/or 3'-ends of the RNA molecules. Southern blot analysis indicates that only one gene is present in the yellow lupin genome. The presence of genes homologous to the LlPRP2 gene was confirmed for three cultivars of yellow lupin and for Lupinus angustifolius. However, LlPRP2 homologues were not detected in Lupinus albus cv. Bac, indicating that this plant may lack the ENOD2 sequence.
    Źródło:
    Acta Biochimica Polonica; 2000, 47, 2; 371-383
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    SRC kinase mRNA transcription changes in testosterone-induced rat ventral prostate lobes under the influence of Epilobium angustifolium extract
    Zmiany liczby transkryptów kinazy SRC w brzusznym płacie prostaty szczurów indukowanych hormonalnie pod wpływem wyciągu z ziela Epilobium angustifolium
    Autorzy:
    Kujawski, R.
    Bartkowiak-Wieczorek, J.
    Bogacz, A.
    Karasiewicz, M.
    Mikolajczak, P.L.
    Czerny, B.
    Mrozikiewicz, P.M.
    Powiązania:
    https://bibliotekanauki.pl/articles/72053.pdf
    Data publikacji:
    2013
    Wydawca:
    Instytut Włókien Naturalnych i Roślin Zielarskich
    Tematy:
    Src kinase
    mRNA
    transcription change
    testosterone
    rat
    ventral prostate lobe
    prostate
    Epilobium angustifolium
    plant extract
    Opis:
    The aim of this study was to investigate the influence of standardized crude aqueous Epilobium angustifolium L. extract [100 mg/kg/day, p.o.] on the expression level of SRC kinase mRNA - a representatives of non-genomics xenobiotics signaling pathway in prostate ventral lobes of testosterone-induced, castrated rats. We have shown that in all analyzed groups induced by testosterone an elevation of SRC kinase mRNA transcription was observed, in comparison to control animals (not receiving the testosterone), (p<0.05). Finasteride in rats induced by testosterone caused the strongest inhibition of SRC mRNA transcription (p<0.05). In rats receiving testosterone and the plant extract a ca. 90% decrease of mRNA level was observed vs. testosterone-induced animals (p<0.05), while in testosterone-induced animals receiving concomitantly E. angustifolium extract and finasteride the observed reduction reached 87.3% (p<0.05). We did not observed, however, any positive feedback between studied plant extract and finasteride in the inhibitory activity (p<0.05). Further experimental studies should be performed in order to the understanding the molecular basis of interactions, the efficacy and safety of tested plant extract.
    Celem pracy było zbadanie wpływu standaryzowanego ekstraktu z ziela Epilobium angustifolium L. [100 mg/kg/dzień, p.o.] na poziom transkrypcji mRNA kinazy SRC - przedstawiciela androgenozależnego, niegenomowego szlaku sygnalizacji komórkowej w brzusznym płacie prostat indukowanych hormonalnie, kastrowanych szczurów. We wszystkich grupach szczurów indukowanych testosteronem zaobserowaliśmy wzrost liczby transkryptów kinazy SRC, w porównaniu do zwierząt kontrolnych (p<0.05). U szczurów indukowanych hormonalnie finasteryd wykazał najsilniejsze właściwości hamujące transkrypcję mRNA (p<0.05). U zwierząt otrzymujących testosteron wraz z wyciągiem z E. angutifolium stwierdziliśmy obniżenie liczby badanych transkryptów o blisko 90%, w porownaniu ze zwierzętami z grupy kontrolnej, indukowanymi testosteronem (p<0.05). U szczurów otrzymujących jednocześnie testosteron i ekstrakt roślinny łącznie z finasterydem redukcja poziomu mRNA kinazy SRC sięgała 87.3% (p<0.05). Nie zaobserwowaliśmy dodatniego sprzężenia w inhibicji transkrypcji badanego mRNA u szczurów otrzymujących badany ekstrakt wraz z finasterydem (p<0.05). Istnieje potrzeba przeprowadzenia dalszych badań zmierzających do wyjaśnienia molekularnego podłoża interakcji, mechanizmu działania oraz skuteczności badanego wciągu roślinnego.
    Źródło:
    Herba Polonica; 2013, 59, 4
    0018-0599
    Pojawia się w:
    Herba Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    The analysis of Fanconi anemia/BRCA pathway genesin laryngeal carcinoma
    Autorzy:
    Szaumkessel, Marcin
    Powiązania:
    https://bibliotekanauki.pl/articles/1401931.pdf
    Data publikacji:
    2014
    Wydawca:
    Index Copernicus International
    Tematy:
    Fanconi anemia/BRCA pathway
    DNA repair
    Laryngeal carcinoma
    DNA methylation
    microRNA
    mRNA level
    FANCA gene
    Opis:
    Summary of Doctoral Thesis/Streszczenie pracy doktorskiej
    Źródło:
    Polski Przegląd Otorynolaryngologiczny; 2014, 3, 4; 250-252
    2084-5308
    2300-7338
    Pojawia się w:
    Polski Przegląd Otorynolaryngologiczny
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Zakażenia wirusem brodawczaka ludzkiego – współczesne metody diagnostyczne
    Human papillomavirus infection – current diagnostic techniques
    Autorzy:
    Skrajna, Adrianna
    Maździarz, Agnieszka
    Reinholz-Jaskólska, Małgorzata
    Powiązania:
    https://bibliotekanauki.pl/articles/1030768.pdf
    Data publikacji:
    2010
    Wydawca:
    Medical Communications
    Tematy:
    Hybrid Capture System
    PCR
    cervical cancer
    human papillomavirus
    mRNA
    rak szyjki macicy
    wirus brodawczaka ludzkiego
    Opis:
    Human papillomavirus (HPV) belongs to the family of DNA viruses. Infection by HPV is associated with the development of benign or malignant lesions within mucosal membrane and skin of the genitals, anus, head and neck. Presence of the virus, particularly of the highly oncogenic variants in epithelial cells of uterine cervix directly influences the development of cervical cancer. In a considerable proportion of cases, HPV infections are asymptomatic and this is probably associated with lack of proliferation phase of viral life cycle, or may be transient, which greatly complicates early detection of the virus. Diagnostic techniques enabling diagnosis of HPV infection include: cytological exam (PAP smear), colposcopy, histological study, as well as increasingly sophisticated molecular techniques, enabling detection of viral DNA when other indices of infection are lacking. These techniques include Southern Blot, Dot Blot, in situ hybridization, polymerase chain reaction (PCR) and Hybrid Capture System I and II (HCI, HCII). Increasingly often authors refer to tests based on isolation of viral mRNA (PreTect HPV-Proofer test). These techniques detect integrated form of HPV, the so-called episomal form of the virus. In general opinion, the presence of HPV in this form is directly associated with progression of lesions developing as a result of this infection. While the PreTect HPV-Proofer test detects a smaller number of cases of HPV infection, its use enables selection of patients most at risk of developing cervical cancer.
    Wirus brodawczaka ludzkiego (HPV) należy do grupy DNA wirusów. Infekcje wirusem brodawczaka ludzkiego wiążą się z występowaniem łagodnych lub złośliwych zmian w obrębie błon śluzowych i skóry narządów płciowych, odbytu, jak również głowy i szyi. Obecność wirusa, szczególnie typów wysokoonkogennych, w komórkach nabłonka szyjki macicy bezpośrednio wpływa na rozwój raka szyjki macicy. Zakażenia HPV w dużym odsetku przebiegają bezobjawowo i jest to najprawdopodobniej związane z niewystąpieniem fazy proliferacyjnej cyklu życiowego wirusa, a także mają charakter przejściowy, co znacznie utrudnia wczesną identyfikację wirusa. Wśród metod diagnostycznych umożliwiających rozpoznanie zakażenia wymienia się badanie cytologiczne, kolposkopię, badanie histologiczne, a także coraz doskonalsze metody molekularne, które pozwalają wykryć wirusowe DNA w przypadkach braku innych wykładników zakażenia. Do metod tych zalicza się: Southern Blot, Dot Blot, hybrydyzacje in situ, polymerase chain reaction (PCR), Hybrid Capture System I i II (HCI, HCII). Coraz częściej wspomina się o testach opartych na izolacji mRNA wirusa HPV (PreTect HPV-Proofer test). Metody te wykrywają zintegrowaną formę HPV, tzw. formę episomalną wirusa. Uważa się, że obecność wirusa HPV w tej postaci jest bezpośrednio związana z progresją zmian powstałych na podłożu tego zakażenia. Co prawda przy pomocy testu PreTect HPV-Proofer wykrywa się mniejszą liczbę zakażeń HPV, ale stosując go, można wyodrębnić grupę pacjentek najbardziej narażonych na raka szyjki macicy.
    Źródło:
    Current Gynecologic Oncology; 2010, 8, 1; 40-46
    2451-0750
    Pojawia się w:
    Current Gynecologic Oncology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    The role of dopamine D2 receptor in the behavioral effects of imipramine - study with the use of antisense oligonucleotides
    Autorzy:
    Dziedzicka-Wasylewska, M.
    Kolasiewicz, W.
    Rogoz, Z.
    Margas, W.
    Maj, J.
    Powiązania:
    https://bibliotekanauki.pl/articles/70213.pdf
    Data publikacji:
    2000
    Wydawca:
    Polskie Towarzystwo Fizjologiczne
    Tematy:
    antisense oligonucleotide
    locomotor activity
    drug
    mRNA
    dopamine D2 receptor
    limbic forebrain
    antidepressant drug
    imipramine
    Źródło:
    Journal of Physiology and Pharmacology; 2000, 51, 3
    0867-5910
    Pojawia się w:
    Journal of Physiology and Pharmacology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Tumor necrosis factor-alpha as a possible auto-/paracrine factor affecting estrous cycle in the cat uterus
    Autorzy:
    Siemieniuch, M.J.
    Ogrodowska, K.
    Ohgawara, H.
    Skarzynski, D.J.
    Okuda, K.
    Powiązania:
    https://bibliotekanauki.pl/articles/31521.pdf
    Data publikacji:
    2010
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    oestrous cycle
    cat
    tumour necrosis factor-alpha
    uterus
    prostaglandin
    tumour cell
    cell differentiation
    mRNA
    gene expression
    Opis:
    Tumor Necrosis Factor-alpha (TNFα) is a pleiotrophic cytokine, affects either normal or tumor cells, and influences cellular differentiation. TNFα role in female reproduction has been proven to be mediated through an influence on prostaglandin (PGs) synthesis and output. To evaluate the possible role of TNFα in an auto-/paracrine regulation in the cat uterus, mRNA expression coding for TNFα and its receptors (TNFR1 and TNFR2), and TNFα protein content at different stages of the estrous cycle were investigated. Additionally, TNFα involvement in PG secretion at different stages of the estrous cycle was investigated by in vitro tissue culture. Gene expressions coding for TNFα and TNFR1 were the highest at diestrus (P < 0.05). TNFα protein expression was the lowest at interestrus (P < 0.05). Nevertheless, TNFR2 was not affected by the estrous stage. TNFα at a dose of 1 ng/ml significantly increased PGF₂α secretion at estrus (P < 0.01) and PGE₂ secretion at diestrus (P < 0.001) after 12h incubation. Overall findings indicate that TNFα locally produced in the cat’s uterus, stimulates PG secretion in an estrous cycle-related manner.
    Źródło:
    Polish Journal of Veterinary Sciences; 2010, 13, 4
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Preliminary study on adverse effects of phenanthrene and its methyl and phenyl derivatives in larval zebrafish, Danio rerio
    Autorzy:
    Wolińska, L.
    Brzuzan, P.
    Woźny, M.
    Góra, M.
    Łuczyński, M. K.
    Podlasz, P.
    Kolwicz, S.
    Piasecka, A.
    Powiązania:
    https://bibliotekanauki.pl/articles/363152.pdf
    Data publikacji:
    2011
    Wydawca:
    Uniwersytet Warmińsko-Mazurski w Olsztynie
    Tematy:
    toksyczność ostra
    CYP1A
    cyp1b1
    geny vtg
    ekspresja mRNA
    stężenie nie wywołujące efektu
    WWA
    Real-Time qPCR
    acute toxicity
    cyp1a genes
    cyp1b1 genes
    vtg genes
    mRNA expression
    No Effect Concentration
    PAHs
    Opis:
    Toxic effects of polycyclic aromatic hydrocarbons (PAHs) have been extensively studied in fish, although knowledge concerning biological activities of phenanthrene and its derivatives remains still incomplete. The aim of this work was to evaluate lethal and sublethal effects of benzo(a)pyrene, phenanthrene and phenanthrene derivatives (1-methylphenanthrene, 4-methylphenanthrene, 1-phenylphenanthrene and 4-phenylphenanthrene) on zebrafish (Danio rerio) larvae. We conducted acute toxicity test, using 96h static renewal exposure to a series of the PAH concentrations (0.05, 0.50, 5.00, 50.00µmol*l-1), to determine the No Effect Concentration (NEC) value for each compound examined. The mean NEC estimates obtained in the study were 5.16۪.45µmol*l-1 (B[a]P), 4.88۪.13µmol*l-1 (Ph), 40.24䔰.93µmol*l-1 (1P-Ph), 47.92ۭ.61µmol*l-1 (1M-Ph), 24.31۱.33µmol*l-1 (4P-Ph) and 3.11۫.01µmol*l-1 (4M-Ph) and suggested the following order of PAH toxicities on Danio rerio larvae: 4M-Ph>Ph˜B[a]P>4PPhP-Ph>1M-Ph. To gain insight into possible molecular mechanisms of apparent toxicity of phenanthrene derivatives on zebrafish larvae, we examined mRNA expression of cyp1a, cyp1b1, and vtg genes in the larvae exposed for 48h to a PAH concentration of 0.50µmol*l-1. Whereas the larvae exposed to each tested PAH displayed many developmental abnormalities (i.e. pericardial and yolk sac edema, dorsal curvature, or tail malformations), no significant upregulation of cyp1a and cyp1b1 mRNA was observed, except for zebrafish exposed to B[a]P. However, significant reduction of vtg mRNA was observed in the larvae exposed to phenanthrene and its 4P- derivative. The results may contribute to the development of a new knowledge about effects of structurally diverse phenanthrene derivatives on vertebrate organisms.
    Źródło:
    Environmental Biotechnology; 2011, 7, 1; 26-33
    1734-4964
    Pojawia się w:
    Environmental Biotechnology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Infection with Echinococcus granulosus [Batsch, 1786] and expression of superoxide dismutase gene at mRNA level in hepatocytes
    Autorzy:
    Kozlowska-Loj, J
    Rzymowska, J.
    Powiązania:
    https://bibliotekanauki.pl/articles/839774.pdf
    Data publikacji:
    2006
    Wydawca:
    Polskie Towarzystwo Parazytologiczne
    Tematy:
    parasitic disease
    pig
    Echinococcus granulosus
    superoxide dismutase
    infection
    gene expression
    mRNA level
    animal disease
    organism
    parasite
    detoxication
    parasitology
    hepatocyte
    Opis:
    Superoxide dismutase (SOD) plays an important role in detoxication of the organism. Its function is to protect the organism against the cytotoxic action of free radicals. The highest expression of superoxide dismutase was observed in the hepatocytes adjacent to the hydatid cyst. The expression of this gene in the hepatocytes of infected livers 5 cm apart from the infection site was slightly lower.
    Źródło:
    Annals of Parasitology; 2006, 52, 4
    0043-5163
    Pojawia się w:
    Annals of Parasitology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    The mRNA sequence polymorphisms of flowering key genes in bolting sensitive or tolerant sugar beet genotypes
    Autorzy:
    Alimirzaee, M.
    Mirzaie-Asl, A.
    Abdollahi, M.R.
    Kolaei, H.E.
    Fasahat, P.
    Powiązania:
    https://bibliotekanauki.pl/articles/79841.pdf
    Data publikacji:
    2017
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    sugar-beet
    Beta vulgaris ssp.maritima
    mRNA
    polymorphism
    flowering
    single nucleotide polymorphism
    genetic control
    RNA extraction
    protein structure prediction
    Źródło:
    BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2017, 98, 3
    0860-7796
    Pojawia się w:
    BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Infection with Echinococcus granulosus [Batsch, 1786] and expression of superoxide dismutase gene at mRNA level in hepatocytes
    Autorzy:
    Kozłowska-Łój, J.
    Rzymowska, J.
    Powiązania:
    https://bibliotekanauki.pl/articles/2146352.pdf
    Data publikacji:
    2006
    Wydawca:
    Polskie Towarzystwo Parazytologiczne
    Tematy:
    parasitic disease
    pig
    Echinococcus granulosus
    superoxide dismutase
    infection
    gene expression
    mRNA level
    animal disease
    organism
    parasite
    detoxication
    parasitology
    hepatocyte
    Opis:
    Superoxide dismutase (SOD) plays an important role in detoxication of the organism. Its function is to protect the organism against the cytotoxic action of free radicals. The highest expression of superoxide dismutase was observed in the hepatocytes adjacent to the hydatid cyst. The expression of this gene in the hepatocytes of infected livers 5 cm apart from the infection site was slightly lower.
    Źródło:
    Wiadomości Parazytologiczne; 2006, 52, 4; 287-289
    0043-5163
    Pojawia się w:
    Wiadomości Parazytologiczne
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    On the biological relevance of alternative splicing in plants: dual function of an Arabidopsis membrane transporter
    Autorzy:
    Doque, P.
    Powiązania:
    https://bibliotekanauki.pl/articles/81202.pdf
    Data publikacji:
    2013
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    eukaryotic gene
    mRNA
    alternative splicing
    plant
    protein function
    proteome
    Arabidopsis thaliana
    auxin transport
    root
    stress tolerance
    plasma membrane
    Źródło:
    BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2013, 94, 3
    0860-7796
    Pojawia się w:
    BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Gonadotropin-releasing hormone and kisspeptin-10 regulate nuclear receptor subfamily 5 group a member 1/catenin beta 1/nuclear receptor subfamily 0 group B member 1 activity in female rat anterior pituitary gland
    Autorzy:
    Zielinska-Gorska, M.
    Gorski, K.
    Biernacka, K.
    Sawosz, E.
    Kaminski, T.
    Gajewska, A.
    Powiązania:
    https://bibliotekanauki.pl/articles/69453.pdf
    Data publikacji:
    2018
    Wydawca:
    Polskie Towarzystwo Fizjologiczne
    Tematy:
    gonadotrophin releasing hormone
    neuronal network
    mRNA expression
    kisspeptin-10
    nuclear receptor
    anterior pituitary gland
    ovariectomy
    luteinizing hormone
    rat
    Źródło:
    Journal of Physiology and Pharmacology; 2018, 69, 3
    0867-5910
    Pojawia się w:
    Journal of Physiology and Pharmacology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    U12 intron is evolutionary conserved in plant nuclear cap binding protein (CBP20) genes and is required for correct pre-miRNA splicing and proper mRNA-protein level
    Autorzy:
    Pieczynski, M.
    Bielewicz, D.
    Dolata, J.
    Szczesniak, M.
    Jarmolowski, A.
    Szweykowska-Kulinska, Z.
    Powiązania:
    https://bibliotekanauki.pl/articles/80411.pdf
    Data publikacji:
    2013
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    conference
    RNA polymerase II
    binding protein
    mRNA
    protein level
    nuclear localization signal
    U12 intron
    Arabidopsis thaliana
    CBP20 gene
    Źródło:
    BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2013, 94, 3
    0860-7796
    Pojawia się w:
    BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Detekcja ekspresji genów drzew leśnych za pomocą mikromacierzy DNA
    Gene-expression in forest-tree species assessed with microarrays as a tool
    Autorzy:
    Nowakowska, J.A.
    Powiązania:
    https://bibliotekanauki.pl/articles/972859.pdf
    Data publikacji:
    2006
    Wydawca:
    Polskie Towarzystwo Leśne
    Tematy:
    mRNA
    ekspresja genow
    genetyka roslin
    mikromacierze DNA
    sekwencja ESTs
    lesnictwo
    wykrywanie
    drzewa lesne
    microarray
    gene−expression
    forest−tree specie
    ESTs
    Opis:
    DNA microarray technology is a powerful tool in functional genomics study of many organisms, the forest−trees included. This method allow to examine simultaneously the changes in expression of thousands of genes and it is based on specific hybridization of cDNA probes from an organism with the DNA library immobilized in an array. The power of this method consists in miniaturization, automation and parallel study of large−scale genome from multiple samples. In forest science, the microarray technology has already been applied in some study of gene−expression in Populus, Pinus and Picea species and the number of new reports is still increasing every year. Since far, some gene−expression have been studied among woody plants in regard of development and growth processes (xylem, adventious−root and zygotic embryo formation, flowering, ripening, shooting of leaves), resistance mechanisms against biotic (fungi, viruses) and abiotic factors (drought, NaCl, elevated CO2 and O3 concentrations). Many practical applications of the microarray technique may concern the early selection in nursery of trees for morphologically valuable traits, the sustainable forest regeneration and the production of genetically transformed species for the chosen trait.
    Źródło:
    Sylwan; 2006, 150, 04; 33-43
    0039-7660
    Pojawia się w:
    Sylwan
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Relation between expression of TNF alpha, iNOS, VEGF mRNA and development of heart failure after experimental myocardial infarction in rats
    Autorzy:
    Heba, G.
    Krzeminski, T.
    Porc, M.
    Grzyb, J.
    Dembinska-Kiec, A.
    Powiązania:
    https://bibliotekanauki.pl/articles/69900.pdf
    Data publikacji:
    2001
    Wydawca:
    Polskie Towarzystwo Fizjologiczne
    Tematy:
    nitric oxide synthase
    tumour necrosis factor-alpha
    cytokine
    ischaemic myocardium
    mRNA
    thoracotomy
    growth factor
    rat
    myocardial infarction
    vascular endothelial growth factor
    Źródło:
    Journal of Physiology and Pharmacology; 2001, 52, 1
    0867-5910
    Pojawia się w:
    Journal of Physiology and Pharmacology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Wybrane czynniki prognostyczne w raku jajnika
    Selected prognostic factors in ovarian cancer
    Autorzy:
    Markowska, Janina
    Bednarek, Wiesława
    Markowska, Anna
    Powiązania:
    https://bibliotekanauki.pl/articles/1030778.pdf
    Data publikacji:
    2010
    Wydawca:
    Medical Communications
    Tematy:
    BRCA1
    VEGF
    prognostic factors in ovarian cancer
    targeted therapy
    mRNA
    markery prognostyczne w raku jajnika
    terapia celowana
    hif-1α
    Opis:
    The authors discuss several predictive factors related to ovarian cancer, both those associated with prognosis and those potentially useful in targeted therapy thereof. Expression of mRNA BRCA1, VEGF and its receptors, CXCR4, HER-2, HIF-1α, COX-2, NM-23, KAI 1, IGF-1R, kisspeptin, neuropilin and Prox-1 gene are discussed. Expression of mRNA BRCA1 is associated not only with hereditary ovarian cancer, but also with its sporadic form. Higher expression correlates with a better response to taxanes. Hopefully, mRNA BRCA1 level in women with sporadic ovarian cancer will become a useful parameter, qualifying patients for a particular chemotherapy protocol. BRMS1 acts as a gene inhibiting development of metastases in ovarian cancer. Levels of mRNA for BRMS1 are lower in late than in early stage ovarian cancer. The VEFG family is involved in angio- and lymphangiogenesis and includes 5 glycoproteins (VEGF-A, VEGF-B, VEGF-C, VEGF-D and VEGF-E), which acting upon receptors VEGFR1-3. Neuropilin 1 plays a role in angiogenesis, while neuropilin 2 modulates lymphangiogenesis, similar to the Prox-1 gene. COX-2 mediates the release of pro-angiogenic factors. HIF-1α expression is associated with resistance of hypoxic cancer cells against cytostatics and radiation. Expression of c-Met correlates with poorly differentiated cancer types. The SDF-1/CXCR4 complex plays a key role in the development of cancer metastases. Survivin is present in cancer cells only, where it inhibits apoptosis and promotes the development of cancer. Metastases suppressor gene (BRMS1) and receptors for VEGF, CXCR4, HER-2, HIF-1α and COX-2, may serve as targets for targeted therapy. At present, an increasing number of studies focus on VEGF receptors. Poor expression of NM-23 and KAI 1 correlate with tumor ability to form metastases. The authors discuss the role of biological markers for clear-cell ovarian cancer (IGF-1R and kisspeptin). Promising topics for future studies are survivin, neuropilin and the Prox-1 gene.
    W artykule omówiono szereg czynników prognostycznych związanych nie tylko z rokowaniem, ale również z możliwością zastosowania terapii tarczowej w raku jajnika. Przedstawiono ekspresję mRNA BRCA1, VEGF i jego receptorów, CXCR4, HER-2, HIF-1α, COX-2, NM-23, KAI 1, IGF-1R, kisspeptyny, neuropiliny i genu Prox-1. Ekspresja mRNA BRCA1 wiąże się nie tylko z dziedzicznym rakiem jajnika, ale również z rakiem sporadycznym. Wyższa ekspresja powoduje lepszą odpowiedź na taksany. Przypuszcza się, że zawartość mRNA BRCA1 u kobiet z rakiem sporadycznym może służyć do selekcji pacjentek do odpowiedniego typu chemioterapii. BRMS1 działa jako gen hamowania przerzutów w raku jajnika. Poziomy mRNA dla BRMS1 są niższe w zaawansowanych stadiach raka niż we wczesnych. Rodzina VEGF związana z angiogenezą i limfangiogenezą składająca się z 5 glikoprotein (VEGF-A, VEGF-B, VEGF-C, VEGF-D, VEGF-E) działa poprzez receptory VEGFR1-3. Neuropilina 1 odgrywa rolę w angiogenezie, a typ drugi moduluje proces limfangiogenezy, podobnie jak gen Prox-1. W uwalnianiu czynników proangiogennych bierze udział COX-2. Ekspresja HIF-1α związana jest z opornością niedotlenionych komórek raka na leczenie chemiczne i napromienianiem. Ekspresja c-Met wiąże się z niższym zróżnicowaniem raka. Kompleks SDF-1/CXCR4 odgrywa kluczową rolę w tworzeniu przerzutów komórek nowotworowych. Surwiwina występująca wyłącznie na komórkach nowotworowych poprzez hamowanie apoptozy promuje rozwój raka. Gen supresji przerzutów (BRMS1), podobnie jak receptory VEGF, CXCR4, HER-2, HIF-1α, COX-2, może być tarczą dla terapii celowanej. Obecnie coraz więcej badań dotyczy inhibitorów VEGF. Obniżona ekspresja NM-23 oraz KAI 1 koreluje ze zdolnością do przerzutowania. Omówiono markery biologiczne dla raka jasnokomórkowego jajnika (IGF-1R i kisspeptynę). Ciekawym obiektem badań może być surwiwina, neuropilina i gen Prox-1.
    Źródło:
    Current Gynecologic Oncology; 2010, 8, 1; 8-20
    2451-0750
    Pojawia się w:
    Current Gynecologic Oncology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Isolation and preliminary sequence characterization of beta-amylase gene promoters in rye [Secale cereale L.]
    Autorzy:
    Sadowski, J
    Rorat, T
    Cooke, R
    Delseny, M
    Powiązania:
    https://bibliotekanauki.pl/articles/2046684.pdf
    Data publikacji:
    1997
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    beta-amylase
    rye
    gene promoter
    homology
    amino acid
    DNA cloning
    prolamin
    Secale cereale
    mRNA
    embryogenesis
    endosperm
    cDNA
    inverse polymerase chain reaction
    Opis:
    The isolation of rye ß-Amy1 and ß-Amy2 gene promoters from nuclear DNA using the inverse polymerase chain reaction (IPCR) technique and characterization of their sequences are presented. The conservation of ß-amylasc coding sequences allowed for simultaneous IPCR amplification of two different promoters with primers designed on the basis of the single known cDNA sequence. Two ß-amylasc gene promoters display a low sequence similarity (47%). Beside consensus TATA and CCAAT boxes, other sequence motives common to both promoters were found. In addition, the homology of amino acid sequences of plant ß-amylases available in the database is discussed.
    Źródło:
    Journal of Applied Genetics; 1997, 38, 3; 241-251
    1234-1983
    Pojawia się w:
    Journal of Applied Genetics
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    The role of iron regulatory proteins in the control of iron metabolism in mammals
    Autorzy:
    Stys, A.
    Starzynski, R.R.
    Lipinski, P.
    Powiązania:
    https://bibliotekanauki.pl/articles/80193.pdf
    Data publikacji:
    2011
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    duodenal enterocyte
    ferritin
    hepatocyte
    hydrogen peroxide
    inflammation
    iron homeostasis
    iron metabolism
    iron regulated protein
    iron regulatory element
    mammal
    mRNA
    nitric oxide
    reticuloendothelial macrophage
    Źródło:
    BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2011, 92, 1
    0860-7796
    Pojawia się w:
    BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Collagen mRNA and fibronectin are increased in healing gastric ulcers in man
    Autorzy:
    Gillessen, A
    Shahin, M.
    Pohle, T.
    Foerster, E.
    Domschke, W.
    Powiązania:
    https://bibliotekanauki.pl/articles/70265.pdf
    Data publikacji:
    1995
    Wydawca:
    Polskie Towarzystwo Fizjologiczne
    Tematy:
    gastric ulcer
    RNA
    submucosa
    gastric mucosa
    collagen
    fibronectin
    human health
    man
    growth regulator
    hybridization
    polyclonal antibody
    epithelial layer
    procollagen mRNA
    antral ulcer
    human placenta
    Źródło:
    Journal of Physiology and Pharmacology; 1995, 46, 1
    0867-5910
    Pojawia się w:
    Journal of Physiology and Pharmacology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Antiviral Activities of Cu2+ Ions in Viral Prevention, Replication, RNA Degradation, and for Antiviral Efficacies of Lytic Virus, ROS-Mediated Virus, Copper Chelation
    Autorzy:
    Ishida, Tsuneo
    Powiązania:
    https://bibliotekanauki.pl/articles/1177808.pdf
    Data publikacji:
    2018
    Wydawca:
    Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
    Tematy:
    Capsid protein
    Copper chelation
    Copper homeostasis
    Copper oxide nanoparticles
    Cu2+ and Cu1+ ions
    DNA/RNA virus
    HSV
    ROS
    Viral replication
    mRNA degradation or decay
    Opis:
    Copper has been known for decades that marked changes of micronutrient homeostasis in the host are accompanied by infection or inflammation. Copper levels in the serum are significantly elevated in response to inflammation that copper accumulates at sites of inflammation. Easily oxidized copper oxide nanoparticles (CuONPs) are widely used as catalysts that the ability of CuONPs to reduce bacterial population and virus application is enhanced. The mechanism of copper-mediated inactivation of herpes simplex virus (HSV) is by which cupric ions oxidatively damage biomolecules. Virus-mediated subjugation and modulation of host lipids during infection that the life cycle of most viruses proceeds through a series of basic steps: binding and internalization, fusion, uncoating, of the viral genome, its replication, assembly of new particles, and budding or release of the newly made viruses. The HIV-1 protein Vpu is an 81-amino-acid (16-kDa) type I which the presence of Vpu leads to the degradation of BST-2 via an endosome-lysosome degradation pathway. Oxidative degradation by a Cu-metalloenzyme, and ubiquitin-mediated degradation of cellular proteins were exploited. Copper can disrupt the lytic cycle of the Coccolithovirus. Lysins represent a novel class of anti-infectives derived from bacteriophage which lysins are bacterial cell wall hydrolytic enzymes that selectively and rapidly kill specific bacteria. Regarding copper induced cellular toxicity, several mechanisms have been proposed based on the formations of ROS by free Cu ions as cupric and cuprous ions can participate in redox reactions. ROS (O2ˉ,･OH, OHˉ), Cu+ and H2O2 play the important roles for viral inactivations. Thujaplicin-copper chelates inhibit influenza virus-induced apoptosis. Pyrrolidine dithiocarbamate as a metal ion binding agent inhibits the activity of the viral proteases of polyprotein processing and RNA replication of HRV. Chelation enables metals are capable of ligand scavenging via complexation, since reverse transcriptase enzyme inhibits the growth and replication of RNA tumor viruses. Thus, copper complex and copper chelation enhance antiviral efficacy.
    Źródło:
    World Scientific News; 2018, 99; 148-168
    2392-2192
    Pojawia się w:
    World Scientific News
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    CYP1A gene expression in adipose fin of rainbow trout (Oncorhynchus mykiss Walbaum) exposed to benzo[a]pyrene
    Autorzy:
    Brzuzan, P.
    Woźny, M.
    Łuczyński, M. K.
    Góra, M.
    Powiązania:
    https://bibliotekanauki.pl/articles/363276.pdf
    Data publikacji:
    2007
    Wydawca:
    Uniwersytet Warmińsko-Mazurski w Olsztynie
    Tematy:
    płetwa tłuszczowa
    benzo(a)piren
    CYP1A mRNA
    skrzela
    pstrąg tęczowy
    real-time PCR
    adipose fin
    benzo(a)pyrene
    gill
    rainbow trout
    Real-Time PCR
    Opis:
    Proximate to the environment, adipose fin of fish may be considered as a lipid storing tissue, and thus can be a target for either waterborne or dietary polycyclic aromatic compounds (PACs). We determined the effects of benzo[a]pyrene (B[a]P), a model PAC member, on CYP1A gene expression in adipose fin and compared that with the effects in gill of juvenile rainbow trout (Oncorhynchus mykiss Walbaum) using the quantitative reverse transcription polymerase chain reaction (Q-RT-PCR). The results of the study demonstrated that constitutive CYP1A mRNA was present in adipose fin of rainbow trout, but the transcripts were far less abundant than those in gill tissue. We confirmed high CYP1A gene induction potential of the gills in rainbow trout injected with benzo[a]pyrene, but also showed moderately and transiently induced CYP1A mRNA in adipose fin. The modest and transitory gene expression may preclude rainbow trout adipose fin CYP1A mRNA levels from using it as an indicator of sustained exposure of fish to the polycyclic aromatic compounds.
    Źródło:
    Environmental Biotechnology; 2007, 3, 1; 20-24
    1734-4964
    Pojawia się w:
    Environmental Biotechnology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
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