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Tytuł:
Wstępne badania w kierunku potwierdzenia obecności krwi ludzkiej w śladach biologicznych na podstawie analizy mRNA
Preliminary examinations aiming at confirming presence of human blood in biological traces by means of mRNA analysis
Autorzy:
Gorzkiewicz, Marta
Grabowska, Małgorzata
Grzybowski, Tomasz
Powiązania:
https://bibliotekanauki.pl/articles/499775.pdf
Data publikacji:
2017
Wydawca:
Centralne Laboratorium Kryminalistyczne Policji
Tematy:
identyfikacja ludzkiej krwi
profilowanie mRNA
hemoglobina
human blood identification
mRNA profiling
haemoglobin
Opis:
Celem projektu było stworzenie specyficznej i zarazem taniej metody służącej do wykrywania ludzkiej krwi w śladach biologicznych opartej na analizie mRNA hemoglobiny z wykorzystaniem reakcji PCR w czasie rzeczywistym, z zastosowaniem niespecyficznego detektora typu SYBR Green. Ostatecznie opracowany test umożliwia jednoczesną analizę krzywych topnienia trzech fragmentów o różnej długości – HBB61, HBA197 i HBB503, oraz dodatkowo genu referencyjnego – mRNA β-aktyny. Jednoznaczna identyfikacja możliwa była już dla 0,1 μl krwi. Metoda jest specyficzna tkankowo i gatunkowo. Analizowane markery mRNA cechują się większą stabilnością w porównaniu do białka hemoglobiny wykrywanego standardowymi metodami. Wynik profilowania mRNA wykazuje wartość predykcyjną odnośnie jakości materiału genetycznego i obecności mieszaniny płynów. Wyniki badań wykonanych w ramach projektu wskazują na potencjalną przydatność markerów HBB i HBA1 w rutynowych badaniach genetyczno-sądowych. Jednak niezbędne jest przeprowadzenie szerszego spektrum eksperymentów walidacyjnych, a szczególnie przeanalizowanie większej liczby rzeczywistych śladów biologicznych, dokładne określenie optymalnej ilości użytego RNA oraz sprawdzenie różnic osobniczych w poziomach ekspresji.
The aim of the project was to develop a specific and at the same time economical method for detecting human blood in biological traces based on analysis of haemoglobin mRNA with use of PCR reaction in real time and non-specific SYBR Green detector. The test, which has eventually been developed enabled simultaneous analysis of melting curves for three fragments of various lengths: HBB61, HBA197 and HBB503, as well as an additional reference gene: mRNA β-actin. A definite identification was possible already for 0,1 μl of blood. The method is tissue and species specific. The analysed mRNA markers are characterized by high stability, as compared to haemoglobin detected by standard methods. The result of mRNA profiling shows the predictive value as regards quality of genetic material and occurrence of mixture of liquids. Results of analyses performed during the project indicate potential usefulness of HBB and HBA1 markers in routine forensic genetic examinations. However, it is necessary to carry out a broader spectrum of validation experiments, and particularly to analyse a larger number of actual biological casework and precisely determining an optimal quantity of RNA and identifying ontogenetic differences in the levels of expression.
Źródło:
Problemy Kryminalistyki; 2017, 295; 33-42
0552-2153
Pojawia się w:
Problemy Kryminalistyki
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The structure of fadL mRNA and its interactions with RybB sRNA
Autorzy:
Groszewska, Agata
Wroblewska, Zuzanna
Olejniczak, Mikolaj
Powiązania:
https://bibliotekanauki.pl/articles/1038749.pdf
Data publikacji:
2016
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
Hfq
sRNA
mRNA
RybB
fadL
coding sequence
Opis:
Small bacterial RNAs (sRNAs) regulate translation by pairing with complementary sequences in their target mRNAs, in a process which is often dependent on the Hfq protein. Here, the secondary structure of a 95-nt long fragment of Salmonella fadL mRNA containing RybB sRNA binding site in the coding region was analyzed. The data indicated local rearrangements in this mRNA structure after the annealing of RybB. The filter retention data had shown that Hfq bound both RybB and the fadL mRNA fragment with tight affinities. Moreover, Hfq increased the rate of RybB annealing to fadL mRNA. These data indicate that Hfq directly participates in RybB interactions with the fadL mRNA.
Źródło:
Acta Biochimica Polonica; 2016, 63, 4; 835-840
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Metabolic enzymes that bind RNA: yet another level of cellular regulatory network?
Autorzy:
Cieśla, Joanna
Powiązania:
https://bibliotekanauki.pl/articles/1041266.pdf
Data publikacji:
2006
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
enzymes
gene expression
mRNA binding
translation regulation
Opis:
Several enzymes that were originally characterized to have one defined function in intermediatory metabolism are now shown to participate in a number of other cellular processes. Multifunctional proteins may be crucial for building of the highly complex networks that maintain the function and structure in the eukaryotic cell possessing a relatively low number of protein-encoding genes. One facet of this phenomenon, on which I will focus in this review, is the interaction of metabolic enzymes with RNA. The list of such enzymes known to be associated with RNA is constantly expanding, but the most intriguing question remains unanswered: are the metabolic enzyme-RNA interactions relevant in the regulation of cell metabolism? It has been proposed that metabolic RNA-binding enzymes participate in general regulatory circuits linking a metabolic function to a regulatory mechanism, similar to the situation of the metabolic enzyme aconitase, which also functions as iron-responsive RNA-binding regulatory element. However, some authors have cautioned that some of such enzymes may merely represent "molecular fossils" of the transition from an RNA to a protein world and that the RNA-binding properties may not have a functional significance. Here I will describe enzymes that have been shown to interact with RNA (in several cases a newly discovered RNA-binding protein has been identified as a well-known metabolic enzyme) and particularly point out those whose ability to interact with RNA seems to have a proven physiological significance. I will also try to depict the molecular switch between an enzyme's metabolic and regulatory functions in cases where such a mechanism has been elucidated. For most of these enzymes relations between their enzymatic functions and RNA metabolism are unclear or seem not to exist. All these enzymes are ancient, as judged by their wide distribution, and participate in fundamental biochemical pathways.
Źródło:
Acta Biochimica Polonica; 2006, 53, 1; 11-32
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Influence of reagent formulation on mRNA quantification by RT-PCR using imported external standard curves
Autorzy:
Farriol, Mireia
Orta, Xavi
Powiązania:
https://bibliotekanauki.pl/articles/1041328.pdf
Data publikacji:
2005
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
mRNA quantification
ornithine decarboxylase
RT-PCR
Opis:
Use of an imported external standard curve is common in real-time quantitative RT-PCR. Two practical strategies for long-term experiments include importing a grand mean standard curve to all accumulated runs or using daily imported standard curves, fixing the slope at the beginning of the experiment and calibrating successive runs with curves generated from this imported slope, adding a single standard that registers the variation in the y-intercept. This study determines the influence that a change in reagent lots has on these two calibration approaches when determining mRNA copy numbers of the ornithine decarboxylase and porphobilinogen deaminase genes. Two sets of determinations were run with the use of lot A and lot B. A marked decrease in the crossing points (Cp) in the standards for both genes at all concentration levels was observed with the change in lots. A grand mean standard curve was generated for each gene and each set and comparisons between the sets were performed. Statistically significant differences were found with respect to the y-intercept but not the slope, suggesting that the change of reagent lot affected the detection sensitivity but not the efficiency of the reaction. The excellent correlation coefficients obtained for these curves for each gene were not achieved when overall data from both sets were combined to generate an overall grand mean standard curve. We conclude that when faced with a change of RT-PCR reagent lot that will affect the detection sensitivity of the method, samples should be calculated with either the daily imported standard curves or with the respective grand mean standard curve for each lot.
Źródło:
Acta Biochimica Polonica; 2005, 52, 4; 845-848
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The nuclear cap-binding protein complex is not essential for nonsense-mediated mRNA decay (NMD) in plants
Autorzy:
Dzikiewicz-Krawczyk, Agnieszka
Piontek, Paulina
Szweykowska-Kulińska, Zofia
Jarmołowski, Artur
Powiązania:
https://bibliotekanauki.pl/articles/1040693.pdf
Data publikacji:
2008
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
nuclear cap-binding protein complex
alternative splicing
mRNA surveillance
NMD
premature terminatin codon
nonsense-mediated mRNA decay
Opis:
In this study we investigated whether in plants, like in mammals, components of the nuclear cap-binding protein complex (CBC) are involved in nonsense-mediated mRNA decay (NMD). We selected several genes producing at least two alternatively spliced mRNA variants: one with a premature termination codon (PTC+) and another without it (PTC-). For each gene the PTC+/PTC- ratio was calculated using RT-PCR and direct sequencing in four Arabidopsis thaliana lines: wild type, the NMD mutant atupf3-1 and two CBC mutants: cbp20 and abh1. Whereas in the NMD mutant the ratios of PTC+/PTC- splice variants were higher than in wild-type plants, the two CBC mutants investigated showed no change in the PTC+/PTC- ratios. Our results suggest that neither CBP20 nor CBP80 is involved in NMD in A. thaliana.
Źródło:
Acta Biochimica Polonica; 2008, 55, 4; 825-828
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Licensed liposomal vaccines and adjuvants in the antigen delivery system
Autorzy:
Krasnopolsky, Yuriy
Pylypenko, Daria
Powiązania:
https://bibliotekanauki.pl/articles/16648151.pdf
Data publikacji:
2022
Wydawca:
Polska Akademia Nauk. Czasopisma i Monografie PAN
Tematy:
vaccine
mRNA
liposomes
adjuvant
virosoma
lipid nanoparticles
Opis:
Liposomes (LSs) are promising nanoparticles with unique properties such as controlled nanosize, large surface area, increased reactivity, and ability to undergo modification. Worldwide, licensed liposomal forms of antibiotics, hormones, antioxidants, cytostatics, ophthalmic drugs, etc., are available on the pharmaceutical market. This review focuses on the adjuvant properties of LSs in the production of vaccines (VACs). LS-VACs have the following advantages: antigens with low immunogenicity can become highly immunogenic; LSs can include both hydrophilic and hydrophobic antigens; LSs allow to achieve a prolonged specific action of antibodies; and LSs reduce the toxicity and pyrogenicity of encapsulated antigens and adjuvants. The immune response is influenced by the composition of the liposomal membrane, physicochemical characteristics of lipids, antigen localization in LSs, interaction of LSs with complement, and a number of proteins, which leads to opsonization. The major requirements for adjuvants are their ability to enhance the immune response, biodegradability, and elimination from the organism, and LSs fully meet these requirements. The effectiveness and safety of LSs as carriers in the antigen delivery system have been proven by the long-term clinical use of licensed vaccines against hepatitis A, influenza, herpes zoster, malaria, and COVID-19.
Źródło:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2022, 103, 4; 409-423
0860-7796
Pojawia się w:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The role of the 5 terminal region of p53 mRNA in the p53 gene expression
Autorzy:
Swiatkowska, Agata
Zydowicz, Paulina
Sroka, Joanna
Ciesiołka, Jerzy
Powiązania:
https://bibliotekanauki.pl/articles/1038716.pdf
Data publikacji:
2016
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
p53
transcription
translation
non-coding region
mRNA
IRES
Opis:
The p53 tumour suppressor protein is one of the major factors responsible for cell cycle regulation and protection against cancer development. This is why it is often referred to as "the guardian of the genome". On the other hand, mutations in the p53 gene are connected with more than 50% of tumours of various types. The thirty-six years of extensive research on the p53 gene and its protein products have shown how sophisticated the p53-based cell system control is. An additional level of complexity of the p53 research is connected with at least twelve p53 isoforms which have been identified in the cell. Importantly, disturbance of the p53 isoforms' expression seems to play a key role in tumorigenesis, cell differentiation and cell response to pathogenic bacteria, and RNA and DNA viruses. Expression of various p53 isoforms results from the usage of different transcription promoters, alternative splicing events and translation initiation from alternative AUG codons. The importance of the 5'-terminal regions of different p53 mRNA transcripts in the multi-level regulation of the p53 gene has recently been documented. In this review we focus on the structural features of these regions and their specific role in the p53 translation initiation process.
Źródło:
Acta Biochimica Polonica; 2016, 63, 4; 645-651
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Differential stability of mitochondrial mRNA in HeLa cells
Autorzy:
Piechota, Janusz
Tomecki, Rafał
Gewartowski, Kamil
Szczęsny, Roman
Dmochowska, Aleksandra
Kudła, Marek
Dybczyńska, Lien
Stepien, Piotr
Bartnik, Ewa
Powiązania:
https://bibliotekanauki.pl/articles/1041282.pdf
Data publikacji:
2006
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
polyadenylation
HeLa
mitochondria
poly(A) polymerase
mRNA turnover
Opis:
The physiological significance and metabolism of oligoadenylated and polyadenylated human mitochondrial mRNAs are not known to date. After study of eight mitochondrial transcripts (ND1, ND2, ND3, ND5, CO1, CO2, ATP6/8 and Cyt. b) we found a direct correlation between the half-lives of mitochondrial mRNAs and their steady-state levels. Investigation of the mt-mRNA decay after thiamphenicol treatment indicated that three transcripts (ND2, ND3 and Cyt. b) are significantly stabilized after inhibition of mitochondrial translation. Careful analysis one of them, ND3, showed that inaccurate processing of the H-strand RNA precursor may occasionally occur between the ND3 and tRNAArg locus leading to synthesis of ND3 mRNAs lacking the STOP codon. However, analysis of the oligo(A) fraction observed in case of the ND3 indicates that partially polyadenylated mRNAs are linked rather to the transcription process than to the translation-dependent deadenylation. Analysis of ND3 mRNA turnover in cells with siRNA-mediated knock-down of the mitochondrial poly(A) polymerase shows that strongly decreased polyadenylation does not markedly affect the decay of this transcript. We present a model where oligoadenylated mitochondrial transcripts are precursors of molecules containing full length poly(A) tails.
Źródło:
Acta Biochimica Polonica; 2006, 53, 1; 157-168
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Contributions of the Hfq protein to translation regulation by small noncoding RNAs binding to the mRNA coding sequence
Autorzy:
Wroblewska, Zuzanna
Olejniczak, Mikolaj
Powiązania:
https://bibliotekanauki.pl/articles/1038726.pdf
Data publikacji:
2016
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
Hfq
sRNA
mRNA
coding sequence
RNase E
ribosome
Opis:
The bacterial Sm-like protein Hfq affects the regulation of translation by small noncoding RNAs (sRNAs). In this way, Hfq participates in the cell adaptation to environmental stress, regulation of cellular metabolism, and bacterial virulence. The majority of known sRNAs bind complementary sequences in the 5'-untranslated mRNA regions. However, recent studies have shown that sRNAs can also target the mRNA coding sequence, even far downstream of the AUG start codon. In this review, we discuss how Hfq contributes to the translation regulation by those sRNAs which bind to the mRNA coding sequence.
Źródło:
Acta Biochimica Polonica; 2016, 63, 4; 701-707
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
COVID-19: szczepienie mRNA dorosłych i dzieci. Niskie ryzyko zapalenia mięśnia sercowego
Autorzy:
Krzystyniak, Krzysztof
Powiązania:
https://bibliotekanauki.pl/articles/2037195.pdf
Data publikacji:
2022-02-16
Wydawca:
Wydawnictwo Naukowe Medyk sp. z o.o.
Tematy:
COVID-19
szczepionki mRNA
zapalenie mięśnia sercowego
zapalenie osierdzia
zespół ostrej niewydolności oddechowej (ARDS)
mRNA vaccines
myocarditis
pericarditis
acute respiratory distress syndrome (ARDS)
Opis:
Aktualnie ocenia się, że bez szczepień prawdopodobnie prawie wszyscy — w tym małe dzieci — w pewnym momencie ich życia zostaną zarażeni koronawirusem SARS-CoV-2. Zaleca się szczepienie przeciw zakażeniom COVID-19 szczepionkami z informacyjnym mRNA dla dorosłych i dla dzieci w wieku 5–11 lat. Wielu rodziców jest niechętnych lub sprzeciwia się zapewnieniu dzieciom tej ochrony. Istnieje niskie ryzyko zapalenia mięśnia sercowego, około 5 przypadków na milion osób otrzymujących szczepionki mRNA anty-COVID-19. Jednak podjęcie negatywnej decyzji o zaszczepieniu nie jest wyborem pozbawionym ryzyka, a raczej jest to wybór poważniejszego ryzyka.
It is now estimated, that without vaccination, it is likely that almost everyone-including young children – will be infected with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) at some point in their lives. Vaccination with messenger RNA (mRNA) anti-COVID-19 vaccine is recommended for adults and for children between 5 and 11 years of age. Many parents are reluctant or opposed to seeking this protection for children. Low risk of myocarditis has been reported, approximately 5 cases per 1,000,000 individuals receiving mRNA COVID-19 vaccines, possibly as high as 1 per 10,000 in young men. However, a choice not to get a vaccine is not a risk-free choice; rather, it’s a choice to take a different and more serious risk.
Źródło:
Gabinet Prywatny; 2022, 280, 01; 5-8
2353-8600
Pojawia się w:
Gabinet Prywatny
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Histopathological characteristics of myocarditis following COVID-19 vaccination: a scoping review
Autorzy:
Kalali, Datis
Anestakis, Doxakis
Powiązania:
https://bibliotekanauki.pl/articles/24987723.pdf
Data publikacji:
2023-10-09
Wydawca:
Gdański Uniwersytet Medyczny
Tematy:
histopathology
myocarditis
myocardial inflammation
COVID-19 vaccine
mRNA vaccines
Opis:
Introduction: Cases of myocarditis in people who were vaccinated against COVID-19 have been reported in the recent years. Nevertheless, the histopathological features and the pathomechanisms in these cases are still unclear. Hence, a scoping review of existing literature was performed to discover the histopathological features of myocarditis induced by the above-mentioned vaccine. Material and Methods: A search was performed in the PubMed, Scopus and EMBASE databases to retrieve the relevant records, involving analyses of biopsy and autopsy specimens. Baseline characteristics of the patients and the histopathological characteristics of the respective specimens were extracted and recorded. Results: Overall, 24 case reports and case series (involving a total of 54 patients) were included in this scoping review. The following signs of inflammation were present in the specimens: lymphocyte infiltration (64.8%), eosinophilic infiltration (29.6%), neutrophil infiltration (3.7%) and giant-cell formation (1.9%). Other features included myocardial tissue necrosis (20.4%), the presence of the SARS-CoV-2 spike protein (16.7%) and microthrombosis (3.7%). Conclusions: The histopathological characteristics of SARS-CoV-2 vaccine-induced myocarditis were heterogenous, the only common characteristic was the presence of lymphocyte infiltration in more than half of the cases. Studies of unreported past cases may provide further insights into the topic.
Źródło:
European Journal of Translational and Clinical Medicine; 2023, 6, 2; 85-90
2657-3148
2657-3156
Pojawia się w:
European Journal of Translational and Clinical Medicine
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Antibody response after SARS-CoV-2 mRNA vaccine in naïve and previously infected healthcare workers
Odpowiedź na przeciwciała po szczepionce mRNA SARS-CoV-2 u uprzednio niezainfekowanych oraz zainfekowanych pracowników medycznych
Autorzy:
Huțanu, Adina
Dobreanu, Minodora
Powiązania:
https://bibliotekanauki.pl/articles/2056546.pdf
Data publikacji:
2022
Wydawca:
Akademia Bialska Nauk Stosowanych im. Jana Pawła II w Białej Podlaskiej
Tematy:
COVID-19 antibody testing
mRNA vaccine
immune response
COVID-19
healthcare workers
testowanie przeciwciał COVID-19
szczepionka mRNA
odpowiedź immunologiczna
pracownicy służby zdrowia
Opis:
Background. Evaluating and monitoring plasma levels of anti-SARS-CoV-2 antibodies in healthcare workers, together with the vaccination of this at-risk population is important for maintaining the viability of the healthcare system, especially during the emergence of new viral variants. The aim of this study is to investigate plasma levels of anti-SARS-CoV-2 antibodies in healthcare providers following full vaccination, in both naïve and previously infected individuals. Material and methods. Complete data was available for 89 healthcare workers from the larger group of 102 initial participants. Plasma was collected at least one month, and no later than two months after the full dose of an mRNA vaccine, and analyzed by determining the total antibody concentration against the spike protein using an ECLIA kit. Results. The degree of humoral-specific immune response was at least 5-fold higher in previously infected healthcare workers compared to naïve persons that received the vaccine only. The highest titer was found in office-based staff, relative to those found in doctors and nurses. However, this difference lacks statistical significance. Among previously infected participants, nurses had significantly higher antibody titers, when compared to doctors. Conclusions. The study revealed a sustained immune response after mRNA vaccine among healthcare workers, with enhanced response in previously infected subjects, highlighting a boosting effect of the vaccine.
Wprowadzenie. Ocena i monitorowanie stężenia przeciwciał anty-SARS-CoV-2 w osoczu pracowników służby zdrowia oraz szczepienia tej szczególnej grupy ryzyka są ważne do utrzymania sprawności systemu opieki zdrowotnej, zwłaszcza w przypadku pojawienia się nowych wariantów wirusa. Celem pracy jest zbadanie poziomu przeciwciał anty-SARS-CoV-2 w osoczu pracowników służby zdrowia po pełnym szczepieniu, zarówno u osób wcześniej niezainfekowanych, jak i zainfekowanych. Materiał i metody. Kompletne dane były dostępne dla 89 pracowników służby zdrowia pochodzących z większej, wstępnej grupy 102 uczestników. Osocze pobierano co najmniej 1 miesiąc, a nie później niż 2 miesiące po podaniu pełnej dawki szczepionki mRNA i analizowano poprzez określenie całkowitego stężenia przeciwciał skierowanych przeciwko białku S przy użyciu zestawu ECLIA. Wyniki. Stopień humoralnej odpowiedzi swoistej był co najmniej 5-krotnie wyższy u uprzednio zakażonych pracowników służby zdrowia w porównaniu z osobami wcześniej niezainfekowanymi, które otrzymały tylko szczepionkę. Wyższe miano stwierdzono wśród osób pracujących w biurze niż u lekarzy i pielęgniarek, jednak różnica ta nie jest istotna statystycznie. Wśród wcześniej zakażonych uczestników pielęgniarki miały znacząco wyższe miana przeciwciał w porównaniu z lekarzami. Wnioski. Badanie wykazało trwałą odpowiedź immunologiczną po podaniu szczepionki mRNA wśród pracowników służby zdrowia, a także zwiększoną odpowiedź u osób wcześniej zakażonych, co podkreśla wzmacniający efekt szczepionki.
Źródło:
Health Problems of Civilization; 2022, 16, 1; 48-56
2353-6942
2354-0265
Pojawia się w:
Health Problems of Civilization
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Cyclopenta[c]phenanthrene induction of CYP1A in brain of rainbow trout (Oncorhynchus mykiss)
Autorzy:
Brzuzan, P.
Woźny, M.
Łuczyński, M. K.
Góra, M.
Ciesielski, S.
Kuźmiński, H.
Powiązania:
https://bibliotekanauki.pl/articles/363272.pdf
Data publikacji:
2007
Wydawca:
Uniwersytet Warmińsko-Mazurski w Olsztynie
Tematy:
benzo(a)piren
mózg
CYP1A
cyklopenta[c]fenantren
absolutna kwantyfikacja mRNA
pstrąg tęczowy
benzo(a)pyrene
brain
cyclopenta[c]phenanthrene
mRNA absolute quantification
rainbow trout
Opis:
We assessed the effects of cyclopenta[c]phenanthrene (CP[c]Ph) and benzo[a]pyrene (B[a]P; positive control) on CYP1A gene expression in brain of juvenile rainbow trout (Oncorhynchus mykiss) using the quantitative reverse transcription polymerase chain reaction (Q-RT-PCR). A group of hatchery raised rainbow trout, with an average body mass of 49.4 g and total length of 15.5 cm were given an intraperitoneal injection (10 mg*kg-1) of either CP[c]Ph or B[a]P in corn oil (2 mg*mi-1 corn oil) or corn oil alone (control). After 24 and 48 h, trout brains were collected for mRNA isolation and analysis. After 24 hours of the exposure, only B[a]P-treated rainbow trout had 10-fold higher number of CYP1A transcripts (mean = 3.63*106 transcripts*µg-1 total RNA) than control fish (3.24*105 transcripts*µg-1 total RNA; Tukey test, P<0.05). After 48 hrs, significantly higher levels of CYP1A expression (Tukey test, P<0.001) were found in either CP[c]Ph- or B[a]P- induced group (1.45*106 and 6.92*106 transcriptsźµg-1 total RNA, respectively) over a control group (mean=1.41*105 transcripts*µg-1 total RNA). The finding that CYP1A in brain tissue was inducible by CP[c]Ph, a polycyclic aromatic hydrocarbon (PAH) of different than B[a]P planar characteristics, may further validate the use of rainbow trout brain CYP1A mRNA levels as a biomarker of PAH exposure.
Źródło:
Environmental Biotechnology; 2007, 3, 1; 10-14
1734-4964
Pojawia się w:
Environmental Biotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Analysis of integrins and vascular endothelial growth factor isoforms mRNA expression in the canine uterus during perimplantation period
Autorzy:
Bukowska, D.
Kempisty, B.
Jackowska, M.
Wozna, M.
Antosik, P.
Piotrowska, H.
Jaskowski, J.M.
Powiązania:
https://bibliotekanauki.pl/articles/31799.pdf
Data publikacji:
2011
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
integrin
vascular endothelial growth factor
isoform
mRNA expression
dog
uterus
pregnancy
bitch
Opis:
Integrins are the major receptors within the extracellular matrix (ECM) that mediate several functions connected with cell life and metabolism, such as cell adhesion, migration, cytoskeletal organization, proliferation, survival, and differentiation. A vascular endothelial growth factor (VEGF) is one of the most important angiogenic factors. It has been suggested that the expression of this gene may play crucial physiological roles in reproductive organs. All investigated endometrial tissues were isolated on day 10-12 after mating. Control bitches, used in this study, were in metestrus, which was determined according to the vaginal cytology and progesterone level in blood. Early pregnancy was verified by flushing the uterine horns with PBS. Total RNA was isolated from the bitches endometrium by means of the Chomczyński and Sacchi method, treated by DNase I, and reverse-transcribed into cDNA. A quantitative analysis of integrins α2b, β2 and β3, VEGF 164, 182 and 188 cDNA was performed by RT-PCR. In results we have shown an increased expression of all investigated genes (integrins α2b, β2 and β3, VEGF 164, 182, and 188) in pregnant bitches uterus as compared to non-pregnant females (P<0.001). Our results indicated that the expression of genes encoding integrins and vascular endothelial growth factors is different in relation to the time of the embryo implantation and it is increased in the first period of this process. This may be associated with the induction of specific mechanisms responsible for receptivity of uterus following the embryo attachment. In addition, all of investigated genes are up-regulated in a pregnancy-specific manner and the increased expression of these genes may regulate the uterus function during the implantation of canine embryos.
Źródło:
Polish Journal of Veterinary Sciences; 2011, 14, 2
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Interaction of three Caenorhabditis elegans isoforms of translation initiation factor eIF4E with mono- and trimethylated mRNA 5 cap analogues.
Autorzy:
Stachelska, Alicja
Wieczorek, Zbigniew
Ruszczyńska, Katarzyna
Stolarski, Ryszard
Pietrzak, Monika
Lamphear, Barry
Rhoads, Robert
Darżynkiewicz, Edward
Jankowska-Anyszka, Marzena
Powiązania:
https://bibliotekanauki.pl/articles/1043732.pdf
Data publikacji:
2002
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
fluorescence quenching
eIF4E
Caenorhabditis elegans
mRNA 5' cap
translation initiation
Opis:
Translation initiation factor eIF4E binds the m7G cap of eukaryotic mRNAs and mediates recruitment of mRNA to the ribosome during cap-dependent translation initiation. This event is the rate-limiting step of translation and a major target for translational control. In the nematode Caenorhabditis elegans, about 70% of genes express mRNAs with an unusual cap structure containing m32,2,7G, which is poorly recognized by mammalian eIF4E. C. elegans expresses five isoforms of eIF4E (IFE-1, IFE-2, etc.). Three of these (IFE-3, IFE-4 and IFE-5) were investigated by means of spectroscopy and structural modelling based on mouse eIF4E bound to m7GDP. Intrinsic fluorescence quenching of Trp residues in the IFEs by iodide ions indicated structural differences between the apo and m7G cap bound proteins. Fluorescence quenching by selected cap analogues showed that only IFE-5 forms specific complexes with both m7G- and m32,2,7G-containing caps (Kas 2×106 M-1 to 7×106 M-1) whereas IFE-3 and IFE-4 discriminated strongly in favor of m7G-containing caps. These spectroscopic results quantitatively confirm earlier qualitative data derived from affinity chromatography. The dependence of Kas on pH indicated optimal cap binding of IFE-3, IFE-4 and IFE-5 at pH 7.2, lower by 0.4 pH units than that of eIF4E from human erythrocytes. These results provide insight into the molecular mechanism of recognition of structurally different caps by the highly homologous IFEs.
Źródło:
Acta Biochimica Polonica; 2002, 49, 3; 671-682
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Localization of the calreticulin gene mRNA in unpollinated and pollinated styles of Petunia hybrida Hort.
Autorzy:
Lenartowska, M
Lenartowski, R.
Bednarska, E.
Powiązania:
https://bibliotekanauki.pl/articles/2041944.pdf
Data publikacji:
2001
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
pistil
Petunia hybrida
gene
pollen tube
transcription
mRNA
protein
calreticulin
transmitting cell
Źródło:
Journal of Applied Genetics; 2001, 42, 1; 15-20
1234-1983
Pojawia się w:
Journal of Applied Genetics
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Evidence for differential effects of glucose and cycloheximide on mRNA levels of peroxisome proliferator-activated receptor- (PPAR-) machinery members: Superinduction of PPAR-γ1 and -γ2 mRNAs
Autorzy:
Rypka, Miroslav
Veselý, Jaroslav
Powiązania:
https://bibliotekanauki.pl/articles/1040405.pdf
Data publikacji:
2010
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
peroxisome proliferator-activated receptor
HepG2 cells
mRNA stability
PPAR-γ coactivator
superinduction
Opis:
Quantitative real-time RT-PCR study was conducted to reveal the effects of normal (5 mmol/l) and high (30 mmol/l) glucose without or with oleate (0.3 mmol/l) on mRNA levels of peroxisome proliferator-activated receptor- (PPAR-)α, -γ1, -γ2, and peroxisome proliferator-activated receptor-γ coactivator- (PGC-)1α and -1β in commercial human hepatoma-derived HepG2 cells maintained under low-serum condition. Significant decrease in PPAR-γ1 and PGC-1α mRNA levels to about 50 % was observed during the first 4 h incubation period. During the next 4 h period, both PPAR-γ1 and PGC-1α mRNAs were partly but significantly restored in high glucose batches. In this period, the presence of the transcriptional inhibitor actinomycin D revealed a significant protective effect of excess glucose on mature PPAR-γ1 and PGC-1α mRNAs. Furthermore, PPAR-γ1 and -γ2 mRNAs were differentially superinduced 1.2-2.5 fold in cells upon the administration of the translational inhibitor cycloheximide. When the cells were co-treated with the combination of cycloheximide and actinomycin D, superinduction was completely suppressed, however. Altogether, the experiments revealed, first, an unexpected protective effect of abundant glucose on PPAR-γ1 and PGC-1α mRNAs in HepG2 cells. Second, we demonstrated cycloheximide-induced, transcription-dependent upregulation of mature PPAR-γ1 and -γ2 mRNAs in HepG2 cells associated with preferential expression of the PPAR-γ2 mRNA variant. The results draw attention to as yet unexplored mechanisms involved in the control of PPAR and PGC genes.
Źródło:
Acta Biochimica Polonica; 2010, 57, 2; 209-215
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The construction of recombinant vectors for a DNA vaccination of rats against fasciolosis
Autorzy:
Kofta, W.
Wedrychowicz, H.
Powiązania:
https://bibliotekanauki.pl/articles/838715.pdf
Data publikacji:
1998
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
parasite
vaccination
cattle
economic loss
fluke
fasciolosis
mRNA
Fasciola hepatica
liver
cysteine proteinase
cDNA
rat
Źródło:
Annals of Parasitology; 1998, 44, 3
0043-5163
Pojawia się w:
Annals of Parasitology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Characterization and expression analysis of the yellow lupin (Lupinus luteus L.) gene coding for nodule specific proline-rich protein.
Autorzy:
Karłowski, Wojciech
Stróżycki, Paweł
Legocki, Andrzej
Powiązania:
https://bibliotekanauki.pl/articles/1044363.pdf
Data publikacji:
2000
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
symbiotic nitrogen fixation
plant gene expression
ENOD2
proline-rich proteins
early nodulins
mRNA degradation
Opis:
The LlPRP2 gene coding for a proline-rich protein shows a high level of similarity to, as well as significant differences from the family of ENOD2 nodule-specific genes. Several sequence motifs with putative regulatory function were identified in the 5' and 3' noncoding regions of the LlPRP2 gene. Northern blot analysis revealed that the expression of the LlPRP2 gene begins 9 days after inoculation of yellow lupin roots with Bradyrhizobium sp. (Lupinus); the expression is restricted to symbiotic nodules and is not detected in other tissues or organs. Detailed hybridization analysis showed that, when expression is activated, the LlPRP2 transcript is modified so as to produce at least three bands and a continuous distribution of decay intermediates. The modification of the LlPRP2 transcript probably involves degradation from the 5'- and/or 3'-ends of the RNA molecules. Southern blot analysis indicates that only one gene is present in the yellow lupin genome. The presence of genes homologous to the LlPRP2 gene was confirmed for three cultivars of yellow lupin and for Lupinus angustifolius. However, LlPRP2 homologues were not detected in Lupinus albus cv. Bac, indicating that this plant may lack the ENOD2 sequence.
Źródło:
Acta Biochimica Polonica; 2000, 47, 2; 371-383
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
SRC kinase mRNA transcription changes in testosterone-induced rat ventral prostate lobes under the influence of Epilobium angustifolium extract
Zmiany liczby transkryptów kinazy SRC w brzusznym płacie prostaty szczurów indukowanych hormonalnie pod wpływem wyciągu z ziela Epilobium angustifolium
Autorzy:
Kujawski, R.
Bartkowiak-Wieczorek, J.
Bogacz, A.
Karasiewicz, M.
Mikolajczak, P.L.
Czerny, B.
Mrozikiewicz, P.M.
Powiązania:
https://bibliotekanauki.pl/articles/72053.pdf
Data publikacji:
2013
Wydawca:
Instytut Włókien Naturalnych i Roślin Zielarskich
Tematy:
Src kinase
mRNA
transcription change
testosterone
rat
ventral prostate lobe
prostate
Epilobium angustifolium
plant extract
Opis:
The aim of this study was to investigate the influence of standardized crude aqueous Epilobium angustifolium L. extract [100 mg/kg/day, p.o.] on the expression level of SRC kinase mRNA - a representatives of non-genomics xenobiotics signaling pathway in prostate ventral lobes of testosterone-induced, castrated rats. We have shown that in all analyzed groups induced by testosterone an elevation of SRC kinase mRNA transcription was observed, in comparison to control animals (not receiving the testosterone), (p<0.05). Finasteride in rats induced by testosterone caused the strongest inhibition of SRC mRNA transcription (p<0.05). In rats receiving testosterone and the plant extract a ca. 90% decrease of mRNA level was observed vs. testosterone-induced animals (p<0.05), while in testosterone-induced animals receiving concomitantly E. angustifolium extract and finasteride the observed reduction reached 87.3% (p<0.05). We did not observed, however, any positive feedback between studied plant extract and finasteride in the inhibitory activity (p<0.05). Further experimental studies should be performed in order to the understanding the molecular basis of interactions, the efficacy and safety of tested plant extract.
Celem pracy było zbadanie wpływu standaryzowanego ekstraktu z ziela Epilobium angustifolium L. [100 mg/kg/dzień, p.o.] na poziom transkrypcji mRNA kinazy SRC - przedstawiciela androgenozależnego, niegenomowego szlaku sygnalizacji komórkowej w brzusznym płacie prostat indukowanych hormonalnie, kastrowanych szczurów. We wszystkich grupach szczurów indukowanych testosteronem zaobserowaliśmy wzrost liczby transkryptów kinazy SRC, w porównaniu do zwierząt kontrolnych (p<0.05). U szczurów indukowanych hormonalnie finasteryd wykazał najsilniejsze właściwości hamujące transkrypcję mRNA (p<0.05). U zwierząt otrzymujących testosteron wraz z wyciągiem z E. angutifolium stwierdziliśmy obniżenie liczby badanych transkryptów o blisko 90%, w porownaniu ze zwierzętami z grupy kontrolnej, indukowanymi testosteronem (p<0.05). U szczurów otrzymujących jednocześnie testosteron i ekstrakt roślinny łącznie z finasterydem redukcja poziomu mRNA kinazy SRC sięgała 87.3% (p<0.05). Nie zaobserwowaliśmy dodatniego sprzężenia w inhibicji transkrypcji badanego mRNA u szczurów otrzymujących badany ekstrakt wraz z finasterydem (p<0.05). Istnieje potrzeba przeprowadzenia dalszych badań zmierzających do wyjaśnienia molekularnego podłoża interakcji, mechanizmu działania oraz skuteczności badanego wciągu roślinnego.
Źródło:
Herba Polonica; 2013, 59, 4
0018-0599
Pojawia się w:
Herba Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The analysis of Fanconi anemia/BRCA pathway genesin laryngeal carcinoma
Autorzy:
Szaumkessel, Marcin
Powiązania:
https://bibliotekanauki.pl/articles/1401931.pdf
Data publikacji:
2014
Wydawca:
Index Copernicus International
Tematy:
Fanconi anemia/BRCA pathway
DNA repair
Laryngeal carcinoma
DNA methylation
microRNA
mRNA level
FANCA gene
Opis:
Summary of Doctoral Thesis/Streszczenie pracy doktorskiej
Źródło:
Polski Przegląd Otorynolaryngologiczny; 2014, 3, 4; 250-252
2084-5308
2300-7338
Pojawia się w:
Polski Przegląd Otorynolaryngologiczny
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Zakażenia wirusem brodawczaka ludzkiego – współczesne metody diagnostyczne
Human papillomavirus infection – current diagnostic techniques
Autorzy:
Skrajna, Adrianna
Maździarz, Agnieszka
Reinholz-Jaskólska, Małgorzata
Powiązania:
https://bibliotekanauki.pl/articles/1030768.pdf
Data publikacji:
2010
Wydawca:
Medical Communications
Tematy:
Hybrid Capture System
PCR
cervical cancer
human papillomavirus
mRNA
rak szyjki macicy
wirus brodawczaka ludzkiego
Opis:
Human papillomavirus (HPV) belongs to the family of DNA viruses. Infection by HPV is associated with the development of benign or malignant lesions within mucosal membrane and skin of the genitals, anus, head and neck. Presence of the virus, particularly of the highly oncogenic variants in epithelial cells of uterine cervix directly influences the development of cervical cancer. In a considerable proportion of cases, HPV infections are asymptomatic and this is probably associated with lack of proliferation phase of viral life cycle, or may be transient, which greatly complicates early detection of the virus. Diagnostic techniques enabling diagnosis of HPV infection include: cytological exam (PAP smear), colposcopy, histological study, as well as increasingly sophisticated molecular techniques, enabling detection of viral DNA when other indices of infection are lacking. These techniques include Southern Blot, Dot Blot, in situ hybridization, polymerase chain reaction (PCR) and Hybrid Capture System I and II (HCI, HCII). Increasingly often authors refer to tests based on isolation of viral mRNA (PreTect HPV-Proofer test). These techniques detect integrated form of HPV, the so-called episomal form of the virus. In general opinion, the presence of HPV in this form is directly associated with progression of lesions developing as a result of this infection. While the PreTect HPV-Proofer test detects a smaller number of cases of HPV infection, its use enables selection of patients most at risk of developing cervical cancer.
Wirus brodawczaka ludzkiego (HPV) należy do grupy DNA wirusów. Infekcje wirusem brodawczaka ludzkiego wiążą się z występowaniem łagodnych lub złośliwych zmian w obrębie błon śluzowych i skóry narządów płciowych, odbytu, jak również głowy i szyi. Obecność wirusa, szczególnie typów wysokoonkogennych, w komórkach nabłonka szyjki macicy bezpośrednio wpływa na rozwój raka szyjki macicy. Zakażenia HPV w dużym odsetku przebiegają bezobjawowo i jest to najprawdopodobniej związane z niewystąpieniem fazy proliferacyjnej cyklu życiowego wirusa, a także mają charakter przejściowy, co znacznie utrudnia wczesną identyfikację wirusa. Wśród metod diagnostycznych umożliwiających rozpoznanie zakażenia wymienia się badanie cytologiczne, kolposkopię, badanie histologiczne, a także coraz doskonalsze metody molekularne, które pozwalają wykryć wirusowe DNA w przypadkach braku innych wykładników zakażenia. Do metod tych zalicza się: Southern Blot, Dot Blot, hybrydyzacje in situ, polymerase chain reaction (PCR), Hybrid Capture System I i II (HCI, HCII). Coraz częściej wspomina się o testach opartych na izolacji mRNA wirusa HPV (PreTect HPV-Proofer test). Metody te wykrywają zintegrowaną formę HPV, tzw. formę episomalną wirusa. Uważa się, że obecność wirusa HPV w tej postaci jest bezpośrednio związana z progresją zmian powstałych na podłożu tego zakażenia. Co prawda przy pomocy testu PreTect HPV-Proofer wykrywa się mniejszą liczbę zakażeń HPV, ale stosując go, można wyodrębnić grupę pacjentek najbardziej narażonych na raka szyjki macicy.
Źródło:
Current Gynecologic Oncology; 2010, 8, 1; 40-46
2451-0750
Pojawia się w:
Current Gynecologic Oncology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The role of dopamine D2 receptor in the behavioral effects of imipramine - study with the use of antisense oligonucleotides
Autorzy:
Dziedzicka-Wasylewska, M.
Kolasiewicz, W.
Rogoz, Z.
Margas, W.
Maj, J.
Powiązania:
https://bibliotekanauki.pl/articles/70213.pdf
Data publikacji:
2000
Wydawca:
Polskie Towarzystwo Fizjologiczne
Tematy:
antisense oligonucleotide
locomotor activity
drug
mRNA
dopamine D2 receptor
limbic forebrain
antidepressant drug
imipramine
Źródło:
Journal of Physiology and Pharmacology; 2000, 51, 3
0867-5910
Pojawia się w:
Journal of Physiology and Pharmacology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Tumor necrosis factor-alpha as a possible auto-/paracrine factor affecting estrous cycle in the cat uterus
Autorzy:
Siemieniuch, M.J.
Ogrodowska, K.
Ohgawara, H.
Skarzynski, D.J.
Okuda, K.
Powiązania:
https://bibliotekanauki.pl/articles/31521.pdf
Data publikacji:
2010
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
oestrous cycle
cat
tumour necrosis factor-alpha
uterus
prostaglandin
tumour cell
cell differentiation
mRNA
gene expression
Opis:
Tumor Necrosis Factor-alpha (TNFα) is a pleiotrophic cytokine, affects either normal or tumor cells, and influences cellular differentiation. TNFα role in female reproduction has been proven to be mediated through an influence on prostaglandin (PGs) synthesis and output. To evaluate the possible role of TNFα in an auto-/paracrine regulation in the cat uterus, mRNA expression coding for TNFα and its receptors (TNFR1 and TNFR2), and TNFα protein content at different stages of the estrous cycle were investigated. Additionally, TNFα involvement in PG secretion at different stages of the estrous cycle was investigated by in vitro tissue culture. Gene expressions coding for TNFα and TNFR1 were the highest at diestrus (P < 0.05). TNFα protein expression was the lowest at interestrus (P < 0.05). Nevertheless, TNFR2 was not affected by the estrous stage. TNFα at a dose of 1 ng/ml significantly increased PGF₂α secretion at estrus (P < 0.01) and PGE₂ secretion at diestrus (P < 0.001) after 12h incubation. Overall findings indicate that TNFα locally produced in the cat’s uterus, stimulates PG secretion in an estrous cycle-related manner.
Źródło:
Polish Journal of Veterinary Sciences; 2010, 13, 4
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Preliminary study on adverse effects of phenanthrene and its methyl and phenyl derivatives in larval zebrafish, Danio rerio
Autorzy:
Wolińska, L.
Brzuzan, P.
Woźny, M.
Góra, M.
Łuczyński, M. K.
Podlasz, P.
Kolwicz, S.
Piasecka, A.
Powiązania:
https://bibliotekanauki.pl/articles/363152.pdf
Data publikacji:
2011
Wydawca:
Uniwersytet Warmińsko-Mazurski w Olsztynie
Tematy:
toksyczność ostra
CYP1A
cyp1b1
geny vtg
ekspresja mRNA
stężenie nie wywołujące efektu
WWA
Real-Time qPCR
acute toxicity
cyp1a genes
cyp1b1 genes
vtg genes
mRNA expression
No Effect Concentration
PAHs
Opis:
Toxic effects of polycyclic aromatic hydrocarbons (PAHs) have been extensively studied in fish, although knowledge concerning biological activities of phenanthrene and its derivatives remains still incomplete. The aim of this work was to evaluate lethal and sublethal effects of benzo(a)pyrene, phenanthrene and phenanthrene derivatives (1-methylphenanthrene, 4-methylphenanthrene, 1-phenylphenanthrene and 4-phenylphenanthrene) on zebrafish (Danio rerio) larvae. We conducted acute toxicity test, using 96h static renewal exposure to a series of the PAH concentrations (0.05, 0.50, 5.00, 50.00µmol*l-1), to determine the No Effect Concentration (NEC) value for each compound examined. The mean NEC estimates obtained in the study were 5.16۪.45µmol*l-1 (B[a]P), 4.88۪.13µmol*l-1 (Ph), 40.24䔰.93µmol*l-1 (1P-Ph), 47.92ۭ.61µmol*l-1 (1M-Ph), 24.31۱.33µmol*l-1 (4P-Ph) and 3.11۫.01µmol*l-1 (4M-Ph) and suggested the following order of PAH toxicities on Danio rerio larvae: 4M-Ph>Ph˜B[a]P>4PPhP-Ph>1M-Ph. To gain insight into possible molecular mechanisms of apparent toxicity of phenanthrene derivatives on zebrafish larvae, we examined mRNA expression of cyp1a, cyp1b1, and vtg genes in the larvae exposed for 48h to a PAH concentration of 0.50µmol*l-1. Whereas the larvae exposed to each tested PAH displayed many developmental abnormalities (i.e. pericardial and yolk sac edema, dorsal curvature, or tail malformations), no significant upregulation of cyp1a and cyp1b1 mRNA was observed, except for zebrafish exposed to B[a]P. However, significant reduction of vtg mRNA was observed in the larvae exposed to phenanthrene and its 4P- derivative. The results may contribute to the development of a new knowledge about effects of structurally diverse phenanthrene derivatives on vertebrate organisms.
Źródło:
Environmental Biotechnology; 2011, 7, 1; 26-33
1734-4964
Pojawia się w:
Environmental Biotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
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