Informacja

Drogi użytkowniku, aplikacja do prawidłowego działania wymaga obsługi JavaScript. Proszę włącz obsługę JavaScript w Twojej przeglądarce.

English (EN)·Polski (PL)·Yкраїнський (UK)
Przejdź do strony domowej biblioteki Centralna Biblioteka Wojskowa Link otwiera się w nowym oknie Logo biblioteki Prolib Integro - strona głównaCentralna Biblioteka Wojskowa

Wyszukujesz frazę "mRNA" wg kryterium: Temat

  Lista filtrów
Wyrównaj
Wyświetlanie 1-15 z 39
Tytuł:
Wstępne badania w kierunku potwierdzenia obecności krwi ludzkiej w śladach biologicznych na podstawie analizy mRNA
Preliminary examinations aiming at confirming presence of human blood in biological traces by means of mRNA analysis
Autorzy:
Gorzkiewicz, Marta
Grabowska, Małgorzata
Grzybowski, Tomasz
Powiązania:
https://bibliotekanauki.pl/articles/499775.pdf
Data publikacji:
2017
Wydawca:
Centralne Laboratorium Kryminalistyczne Policji
Tematy:
identyfikacja ludzkiej krwi
profilowanie mRNA
hemoglobina
human blood identification
mRNA profiling
haemoglobin
Opis:
Celem projektu było stworzenie specyficznej i zarazem taniej metody służącej do wykrywania ludzkiej krwi w śladach biologicznych opartej na analizie mRNA hemoglobiny z wykorzystaniem reakcji PCR w czasie rzeczywistym, z zastosowaniem niespecyficznego detektora typu SYBR Green. Ostatecznie opracowany test umożliwia jednoczesną analizę krzywych topnienia trzech fragmentów o różnej długości – HBB61, HBA197 i HBB503, oraz dodatkowo genu referencyjnego – mRNA β-aktyny. Jednoznaczna identyfikacja możliwa była już dla 0,1 μl krwi. Metoda jest specyficzna tkankowo i gatunkowo. Analizowane markery mRNA cechują się większą stabilnością w porównaniu do białka hemoglobiny wykrywanego standardowymi metodami. Wynik profilowania mRNA wykazuje wartość predykcyjną odnośnie jakości materiału genetycznego i obecności mieszaniny płynów. Wyniki badań wykonanych w ramach projektu wskazują na potencjalną przydatność markerów HBB i HBA1 w rutynowych badaniach genetyczno-sądowych. Jednak niezbędne jest przeprowadzenie szerszego spektrum eksperymentów walidacyjnych, a szczególnie przeanalizowanie większej liczby rzeczywistych śladów biologicznych, dokładne określenie optymalnej ilości użytego RNA oraz sprawdzenie różnic osobniczych w poziomach ekspresji.
The aim of the project was to develop a specific and at the same time economical method for detecting human blood in biological traces based on analysis of haemoglobin mRNA with use of PCR reaction in real time and non-specific SYBR Green detector. The test, which has eventually been developed enabled simultaneous analysis of melting curves for three fragments of various lengths: HBB61, HBA197 and HBB503, as well as an additional reference gene: mRNA β-actin. A definite identification was possible already for 0,1 μl of blood. The method is tissue and species specific. The analysed mRNA markers are characterized by high stability, as compared to haemoglobin detected by standard methods. The result of mRNA profiling shows the predictive value as regards quality of genetic material and occurrence of mixture of liquids. Results of analyses performed during the project indicate potential usefulness of HBB and HBA1 markers in routine forensic genetic examinations. However, it is necessary to carry out a broader spectrum of validation experiments, and particularly to analyse a larger number of actual biological casework and precisely determining an optimal quantity of RNA and identifying ontogenetic differences in the levels of expression.
Źródło:
Problemy Kryminalistyki; 2017, 295; 33-42
0552-2153
Pojawia się w:
Problemy Kryminalistyki
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The structure of fadL mRNA and its interactions with RybB sRNA
Autorzy:
Groszewska, Agata
Wroblewska, Zuzanna
Olejniczak, Mikolaj
Powiązania:
https://bibliotekanauki.pl/articles/1038749.pdf
Data publikacji:
2016
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
Hfq
sRNA
mRNA
RybB
fadL
coding sequence
Opis:
Small bacterial RNAs (sRNAs) regulate translation by pairing with complementary sequences in their target mRNAs, in a process which is often dependent on the Hfq protein. Here, the secondary structure of a 95-nt long fragment of Salmonella fadL mRNA containing RybB sRNA binding site in the coding region was analyzed. The data indicated local rearrangements in this mRNA structure after the annealing of RybB. The filter retention data had shown that Hfq bound both RybB and the fadL mRNA fragment with tight affinities. Moreover, Hfq increased the rate of RybB annealing to fadL mRNA. These data indicate that Hfq directly participates in RybB interactions with the fadL mRNA.
Źródło:
Acta Biochimica Polonica; 2016, 63, 4; 835-840
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Metabolic enzymes that bind RNA: yet another level of cellular regulatory network?
Autorzy:
Cieśla, Joanna
Powiązania:
https://bibliotekanauki.pl/articles/1041266.pdf
Data publikacji:
2006
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
enzymes
gene expression
mRNA binding
translation regulation
Opis:
Several enzymes that were originally characterized to have one defined function in intermediatory metabolism are now shown to participate in a number of other cellular processes. Multifunctional proteins may be crucial for building of the highly complex networks that maintain the function and structure in the eukaryotic cell possessing a relatively low number of protein-encoding genes. One facet of this phenomenon, on which I will focus in this review, is the interaction of metabolic enzymes with RNA. The list of such enzymes known to be associated with RNA is constantly expanding, but the most intriguing question remains unanswered: are the metabolic enzyme-RNA interactions relevant in the regulation of cell metabolism? It has been proposed that metabolic RNA-binding enzymes participate in general regulatory circuits linking a metabolic function to a regulatory mechanism, similar to the situation of the metabolic enzyme aconitase, which also functions as iron-responsive RNA-binding regulatory element. However, some authors have cautioned that some of such enzymes may merely represent "molecular fossils" of the transition from an RNA to a protein world and that the RNA-binding properties may not have a functional significance. Here I will describe enzymes that have been shown to interact with RNA (in several cases a newly discovered RNA-binding protein has been identified as a well-known metabolic enzyme) and particularly point out those whose ability to interact with RNA seems to have a proven physiological significance. I will also try to depict the molecular switch between an enzyme's metabolic and regulatory functions in cases where such a mechanism has been elucidated. For most of these enzymes relations between their enzymatic functions and RNA metabolism are unclear or seem not to exist. All these enzymes are ancient, as judged by their wide distribution, and participate in fundamental biochemical pathways.
Źródło:
Acta Biochimica Polonica; 2006, 53, 1; 11-32
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Influence of reagent formulation on mRNA quantification by RT-PCR using imported external standard curves
Autorzy:
Farriol, Mireia
Orta, Xavi
Powiązania:
https://bibliotekanauki.pl/articles/1041328.pdf
Data publikacji:
2005
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
mRNA quantification
ornithine decarboxylase
RT-PCR
Opis:
Use of an imported external standard curve is common in real-time quantitative RT-PCR. Two practical strategies for long-term experiments include importing a grand mean standard curve to all accumulated runs or using daily imported standard curves, fixing the slope at the beginning of the experiment and calibrating successive runs with curves generated from this imported slope, adding a single standard that registers the variation in the y-intercept. This study determines the influence that a change in reagent lots has on these two calibration approaches when determining mRNA copy numbers of the ornithine decarboxylase and porphobilinogen deaminase genes. Two sets of determinations were run with the use of lot A and lot B. A marked decrease in the crossing points (Cp) in the standards for both genes at all concentration levels was observed with the change in lots. A grand mean standard curve was generated for each gene and each set and comparisons between the sets were performed. Statistically significant differences were found with respect to the y-intercept but not the slope, suggesting that the change of reagent lot affected the detection sensitivity but not the efficiency of the reaction. The excellent correlation coefficients obtained for these curves for each gene were not achieved when overall data from both sets were combined to generate an overall grand mean standard curve. We conclude that when faced with a change of RT-PCR reagent lot that will affect the detection sensitivity of the method, samples should be calculated with either the daily imported standard curves or with the respective grand mean standard curve for each lot.
Źródło:
Acta Biochimica Polonica; 2005, 52, 4; 845-848
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The nuclear cap-binding protein complex is not essential for nonsense-mediated mRNA decay (NMD) in plants
Autorzy:
Dzikiewicz-Krawczyk, Agnieszka
Piontek, Paulina
Szweykowska-Kulińska, Zofia
Jarmołowski, Artur
Powiązania:
https://bibliotekanauki.pl/articles/1040693.pdf
Data publikacji:
2008
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
nuclear cap-binding protein complex
alternative splicing
mRNA surveillance
NMD
premature terminatin codon
nonsense-mediated mRNA decay
Opis:
In this study we investigated whether in plants, like in mammals, components of the nuclear cap-binding protein complex (CBC) are involved in nonsense-mediated mRNA decay (NMD). We selected several genes producing at least two alternatively spliced mRNA variants: one with a premature termination codon (PTC+) and another without it (PTC-). For each gene the PTC+/PTC- ratio was calculated using RT-PCR and direct sequencing in four Arabidopsis thaliana lines: wild type, the NMD mutant atupf3-1 and two CBC mutants: cbp20 and abh1. Whereas in the NMD mutant the ratios of PTC+/PTC- splice variants were higher than in wild-type plants, the two CBC mutants investigated showed no change in the PTC+/PTC- ratios. Our results suggest that neither CBP20 nor CBP80 is involved in NMD in A. thaliana.
Źródło:
Acta Biochimica Polonica; 2008, 55, 4; 825-828
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Licensed liposomal vaccines and adjuvants in the antigen delivery system
Autorzy:
Krasnopolsky, Yuriy
Pylypenko, Daria
Powiązania:
https://bibliotekanauki.pl/articles/16648151.pdf
Data publikacji:
2022
Wydawca:
Polska Akademia Nauk. Czasopisma i Monografie PAN
Tematy:
vaccine
mRNA
liposomes
adjuvant
virosoma
lipid nanoparticles
Opis:
Liposomes (LSs) are promising nanoparticles with unique properties such as controlled nanosize, large surface area, increased reactivity, and ability to undergo modification. Worldwide, licensed liposomal forms of antibiotics, hormones, antioxidants, cytostatics, ophthalmic drugs, etc., are available on the pharmaceutical market. This review focuses on the adjuvant properties of LSs in the production of vaccines (VACs). LS-VACs have the following advantages: antigens with low immunogenicity can become highly immunogenic; LSs can include both hydrophilic and hydrophobic antigens; LSs allow to achieve a prolonged specific action of antibodies; and LSs reduce the toxicity and pyrogenicity of encapsulated antigens and adjuvants. The immune response is influenced by the composition of the liposomal membrane, physicochemical characteristics of lipids, antigen localization in LSs, interaction of LSs with complement, and a number of proteins, which leads to opsonization. The major requirements for adjuvants are their ability to enhance the immune response, biodegradability, and elimination from the organism, and LSs fully meet these requirements. The effectiveness and safety of LSs as carriers in the antigen delivery system have been proven by the long-term clinical use of licensed vaccines against hepatitis A, influenza, herpes zoster, malaria, and COVID-19.
Źródło:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2022, 103, 4; 409-423
0860-7796
Pojawia się w:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The role of the 5 terminal region of p53 mRNA in the p53 gene expression
Autorzy:
Swiatkowska, Agata
Zydowicz, Paulina
Sroka, Joanna
Ciesiołka, Jerzy
Powiązania:
https://bibliotekanauki.pl/articles/1038716.pdf
Data publikacji:
2016
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
p53
transcription
translation
non-coding region
mRNA
IRES
Opis:
The p53 tumour suppressor protein is one of the major factors responsible for cell cycle regulation and protection against cancer development. This is why it is often referred to as "the guardian of the genome". On the other hand, mutations in the p53 gene are connected with more than 50% of tumours of various types. The thirty-six years of extensive research on the p53 gene and its protein products have shown how sophisticated the p53-based cell system control is. An additional level of complexity of the p53 research is connected with at least twelve p53 isoforms which have been identified in the cell. Importantly, disturbance of the p53 isoforms' expression seems to play a key role in tumorigenesis, cell differentiation and cell response to pathogenic bacteria, and RNA and DNA viruses. Expression of various p53 isoforms results from the usage of different transcription promoters, alternative splicing events and translation initiation from alternative AUG codons. The importance of the 5'-terminal regions of different p53 mRNA transcripts in the multi-level regulation of the p53 gene has recently been documented. In this review we focus on the structural features of these regions and their specific role in the p53 translation initiation process.
Źródło:
Acta Biochimica Polonica; 2016, 63, 4; 645-651
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Differential stability of mitochondrial mRNA in HeLa cells
Autorzy:
Piechota, Janusz
Tomecki, Rafał
Gewartowski, Kamil
Szczęsny, Roman
Dmochowska, Aleksandra
Kudła, Marek
Dybczyńska, Lien
Stepien, Piotr
Bartnik, Ewa
Powiązania:
https://bibliotekanauki.pl/articles/1041282.pdf
Data publikacji:
2006
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
polyadenylation
HeLa
mitochondria
poly(A) polymerase
mRNA turnover
Opis:
The physiological significance and metabolism of oligoadenylated and polyadenylated human mitochondrial mRNAs are not known to date. After study of eight mitochondrial transcripts (ND1, ND2, ND3, ND5, CO1, CO2, ATP6/8 and Cyt. b) we found a direct correlation between the half-lives of mitochondrial mRNAs and their steady-state levels. Investigation of the mt-mRNA decay after thiamphenicol treatment indicated that three transcripts (ND2, ND3 and Cyt. b) are significantly stabilized after inhibition of mitochondrial translation. Careful analysis one of them, ND3, showed that inaccurate processing of the H-strand RNA precursor may occasionally occur between the ND3 and tRNAArg locus leading to synthesis of ND3 mRNAs lacking the STOP codon. However, analysis of the oligo(A) fraction observed in case of the ND3 indicates that partially polyadenylated mRNAs are linked rather to the transcription process than to the translation-dependent deadenylation. Analysis of ND3 mRNA turnover in cells with siRNA-mediated knock-down of the mitochondrial poly(A) polymerase shows that strongly decreased polyadenylation does not markedly affect the decay of this transcript. We present a model where oligoadenylated mitochondrial transcripts are precursors of molecules containing full length poly(A) tails.
Źródło:
Acta Biochimica Polonica; 2006, 53, 1; 157-168
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Contributions of the Hfq protein to translation regulation by small noncoding RNAs binding to the mRNA coding sequence
Autorzy:
Wroblewska, Zuzanna
Olejniczak, Mikolaj
Powiązania:
https://bibliotekanauki.pl/articles/1038726.pdf
Data publikacji:
2016
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
Hfq
sRNA
mRNA
coding sequence
RNase E
ribosome
Opis:
The bacterial Sm-like protein Hfq affects the regulation of translation by small noncoding RNAs (sRNAs). In this way, Hfq participates in the cell adaptation to environmental stress, regulation of cellular metabolism, and bacterial virulence. The majority of known sRNAs bind complementary sequences in the 5'-untranslated mRNA regions. However, recent studies have shown that sRNAs can also target the mRNA coding sequence, even far downstream of the AUG start codon. In this review, we discuss how Hfq contributes to the translation regulation by those sRNAs which bind to the mRNA coding sequence.
Źródło:
Acta Biochimica Polonica; 2016, 63, 4; 701-707
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
COVID-19: szczepienie mRNA dorosłych i dzieci. Niskie ryzyko zapalenia mięśnia sercowego
Autorzy:
Krzystyniak, Krzysztof
Powiązania:
https://bibliotekanauki.pl/articles/2037195.pdf
Data publikacji:
2022-02-16
Wydawca:
Wydawnictwo Naukowe Medyk sp. z o.o.
Tematy:
COVID-19
szczepionki mRNA
zapalenie mięśnia sercowego
zapalenie osierdzia
zespół ostrej niewydolności oddechowej (ARDS)
mRNA vaccines
myocarditis
pericarditis
acute respiratory distress syndrome (ARDS)
Opis:
Aktualnie ocenia się, że bez szczepień prawdopodobnie prawie wszyscy — w tym małe dzieci — w pewnym momencie ich życia zostaną zarażeni koronawirusem SARS-CoV-2. Zaleca się szczepienie przeciw zakażeniom COVID-19 szczepionkami z informacyjnym mRNA dla dorosłych i dla dzieci w wieku 5–11 lat. Wielu rodziców jest niechętnych lub sprzeciwia się zapewnieniu dzieciom tej ochrony. Istnieje niskie ryzyko zapalenia mięśnia sercowego, około 5 przypadków na milion osób otrzymujących szczepionki mRNA anty-COVID-19. Jednak podjęcie negatywnej decyzji o zaszczepieniu nie jest wyborem pozbawionym ryzyka, a raczej jest to wybór poważniejszego ryzyka.
It is now estimated, that without vaccination, it is likely that almost everyone-including young children – will be infected with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) at some point in their lives. Vaccination with messenger RNA (mRNA) anti-COVID-19 vaccine is recommended for adults and for children between 5 and 11 years of age. Many parents are reluctant or opposed to seeking this protection for children. Low risk of myocarditis has been reported, approximately 5 cases per 1,000,000 individuals receiving mRNA COVID-19 vaccines, possibly as high as 1 per 10,000 in young men. However, a choice not to get a vaccine is not a risk-free choice; rather, it’s a choice to take a different and more serious risk.
Źródło:
Gabinet Prywatny; 2022, 280, 01; 5-8
2353-8600
Pojawia się w:
Gabinet Prywatny
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Histopathological characteristics of myocarditis following COVID-19 vaccination: a scoping review
Autorzy:
Kalali, Datis
Anestakis, Doxakis
Powiązania:
https://bibliotekanauki.pl/articles/24987723.pdf
Data publikacji:
2023-10-09
Wydawca:
Gdański Uniwersytet Medyczny
Tematy:
histopathology
myocarditis
myocardial inflammation
COVID-19 vaccine
mRNA vaccines
Opis:
Introduction: Cases of myocarditis in people who were vaccinated against COVID-19 have been reported in the recent years. Nevertheless, the histopathological features and the pathomechanisms in these cases are still unclear. Hence, a scoping review of existing literature was performed to discover the histopathological features of myocarditis induced by the above-mentioned vaccine. Material and Methods: A search was performed in the PubMed, Scopus and EMBASE databases to retrieve the relevant records, involving analyses of biopsy and autopsy specimens. Baseline characteristics of the patients and the histopathological characteristics of the respective specimens were extracted and recorded. Results: Overall, 24 case reports and case series (involving a total of 54 patients) were included in this scoping review. The following signs of inflammation were present in the specimens: lymphocyte infiltration (64.8%), eosinophilic infiltration (29.6%), neutrophil infiltration (3.7%) and giant-cell formation (1.9%). Other features included myocardial tissue necrosis (20.4%), the presence of the SARS-CoV-2 spike protein (16.7%) and microthrombosis (3.7%). Conclusions: The histopathological characteristics of SARS-CoV-2 vaccine-induced myocarditis were heterogenous, the only common characteristic was the presence of lymphocyte infiltration in more than half of the cases. Studies of unreported past cases may provide further insights into the topic.
Źródło:
European Journal of Translational and Clinical Medicine; 2023, 6, 2; 85-90
2657-3148
2657-3156
Pojawia się w:
European Journal of Translational and Clinical Medicine
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Antibody response after SARS-CoV-2 mRNA vaccine in naïve and previously infected healthcare workers
Odpowiedź na przeciwciała po szczepionce mRNA SARS-CoV-2 u uprzednio niezainfekowanych oraz zainfekowanych pracowników medycznych
Autorzy:
Huțanu, Adina
Dobreanu, Minodora
Powiązania:
https://bibliotekanauki.pl/articles/2056546.pdf
Data publikacji:
2022
Wydawca:
Akademia Bialska Nauk Stosowanych im. Jana Pawła II w Białej Podlaskiej
Tematy:
COVID-19 antibody testing
mRNA vaccine
immune response
COVID-19
healthcare workers
testowanie przeciwciał COVID-19
szczepionka mRNA
odpowiedź immunologiczna
pracownicy służby zdrowia
Opis:
Background. Evaluating and monitoring plasma levels of anti-SARS-CoV-2 antibodies in healthcare workers, together with the vaccination of this at-risk population is important for maintaining the viability of the healthcare system, especially during the emergence of new viral variants. The aim of this study is to investigate plasma levels of anti-SARS-CoV-2 antibodies in healthcare providers following full vaccination, in both naïve and previously infected individuals. Material and methods. Complete data was available for 89 healthcare workers from the larger group of 102 initial participants. Plasma was collected at least one month, and no later than two months after the full dose of an mRNA vaccine, and analyzed by determining the total antibody concentration against the spike protein using an ECLIA kit. Results. The degree of humoral-specific immune response was at least 5-fold higher in previously infected healthcare workers compared to naïve persons that received the vaccine only. The highest titer was found in office-based staff, relative to those found in doctors and nurses. However, this difference lacks statistical significance. Among previously infected participants, nurses had significantly higher antibody titers, when compared to doctors. Conclusions. The study revealed a sustained immune response after mRNA vaccine among healthcare workers, with enhanced response in previously infected subjects, highlighting a boosting effect of the vaccine.
Wprowadzenie. Ocena i monitorowanie stężenia przeciwciał anty-SARS-CoV-2 w osoczu pracowników służby zdrowia oraz szczepienia tej szczególnej grupy ryzyka są ważne do utrzymania sprawności systemu opieki zdrowotnej, zwłaszcza w przypadku pojawienia się nowych wariantów wirusa. Celem pracy jest zbadanie poziomu przeciwciał anty-SARS-CoV-2 w osoczu pracowników służby zdrowia po pełnym szczepieniu, zarówno u osób wcześniej niezainfekowanych, jak i zainfekowanych. Materiał i metody. Kompletne dane były dostępne dla 89 pracowników służby zdrowia pochodzących z większej, wstępnej grupy 102 uczestników. Osocze pobierano co najmniej 1 miesiąc, a nie później niż 2 miesiące po podaniu pełnej dawki szczepionki mRNA i analizowano poprzez określenie całkowitego stężenia przeciwciał skierowanych przeciwko białku S przy użyciu zestawu ECLIA. Wyniki. Stopień humoralnej odpowiedzi swoistej był co najmniej 5-krotnie wyższy u uprzednio zakażonych pracowników służby zdrowia w porównaniu z osobami wcześniej niezainfekowanymi, które otrzymały tylko szczepionkę. Wyższe miano stwierdzono wśród osób pracujących w biurze niż u lekarzy i pielęgniarek, jednak różnica ta nie jest istotna statystycznie. Wśród wcześniej zakażonych uczestników pielęgniarki miały znacząco wyższe miana przeciwciał w porównaniu z lekarzami. Wnioski. Badanie wykazało trwałą odpowiedź immunologiczną po podaniu szczepionki mRNA wśród pracowników służby zdrowia, a także zwiększoną odpowiedź u osób wcześniej zakażonych, co podkreśla wzmacniający efekt szczepionki.
Źródło:
Health Problems of Civilization; 2022, 16, 1; 48-56
2353-6942
2354-0265
Pojawia się w:
Health Problems of Civilization
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Cyclopenta[c]phenanthrene induction of CYP1A in brain of rainbow trout (Oncorhynchus mykiss)
Autorzy:
Brzuzan, P.
Woźny, M.
Łuczyński, M. K.
Góra, M.
Ciesielski, S.
Kuźmiński, H.
Powiązania:
https://bibliotekanauki.pl/articles/363272.pdf
Data publikacji:
2007
Wydawca:
Uniwersytet Warmińsko-Mazurski w Olsztynie
Tematy:
benzo(a)piren
mózg
CYP1A
cyklopenta[c]fenantren
absolutna kwantyfikacja mRNA
pstrąg tęczowy
benzo(a)pyrene
brain
cyclopenta[c]phenanthrene
mRNA absolute quantification
rainbow trout
Opis:
We assessed the effects of cyclopenta[c]phenanthrene (CP[c]Ph) and benzo[a]pyrene (B[a]P; positive control) on CYP1A gene expression in brain of juvenile rainbow trout (Oncorhynchus mykiss) using the quantitative reverse transcription polymerase chain reaction (Q-RT-PCR). A group of hatchery raised rainbow trout, with an average body mass of 49.4 g and total length of 15.5 cm were given an intraperitoneal injection (10 mg*kg-1) of either CP[c]Ph or B[a]P in corn oil (2 mg*mi-1 corn oil) or corn oil alone (control). After 24 and 48 h, trout brains were collected for mRNA isolation and analysis. After 24 hours of the exposure, only B[a]P-treated rainbow trout had 10-fold higher number of CYP1A transcripts (mean = 3.63*106 transcripts*µg-1 total RNA) than control fish (3.24*105 transcripts*µg-1 total RNA; Tukey test, P<0.05). After 48 hrs, significantly higher levels of CYP1A expression (Tukey test, P<0.001) were found in either CP[c]Ph- or B[a]P- induced group (1.45*106 and 6.92*106 transcriptsźµg-1 total RNA, respectively) over a control group (mean=1.41*105 transcripts*µg-1 total RNA). The finding that CYP1A in brain tissue was inducible by CP[c]Ph, a polycyclic aromatic hydrocarbon (PAH) of different than B[a]P planar characteristics, may further validate the use of rainbow trout brain CYP1A mRNA levels as a biomarker of PAH exposure.
Źródło:
Environmental Biotechnology; 2007, 3, 1; 10-14
1734-4964
Pojawia się w:
Environmental Biotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Analysis of integrins and vascular endothelial growth factor isoforms mRNA expression in the canine uterus during perimplantation period
Autorzy:
Bukowska, D.
Kempisty, B.
Jackowska, M.
Wozna, M.
Antosik, P.
Piotrowska, H.
Jaskowski, J.M.
Powiązania:
https://bibliotekanauki.pl/articles/31799.pdf
Data publikacji:
2011
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
integrin
vascular endothelial growth factor
isoform
mRNA expression
dog
uterus
pregnancy
bitch
Opis:
Integrins are the major receptors within the extracellular matrix (ECM) that mediate several functions connected with cell life and metabolism, such as cell adhesion, migration, cytoskeletal organization, proliferation, survival, and differentiation. A vascular endothelial growth factor (VEGF) is one of the most important angiogenic factors. It has been suggested that the expression of this gene may play crucial physiological roles in reproductive organs. All investigated endometrial tissues were isolated on day 10-12 after mating. Control bitches, used in this study, were in metestrus, which was determined according to the vaginal cytology and progesterone level in blood. Early pregnancy was verified by flushing the uterine horns with PBS. Total RNA was isolated from the bitches endometrium by means of the Chomczyński and Sacchi method, treated by DNase I, and reverse-transcribed into cDNA. A quantitative analysis of integrins α2b, β2 and β3, VEGF 164, 182 and 188 cDNA was performed by RT-PCR. In results we have shown an increased expression of all investigated genes (integrins α2b, β2 and β3, VEGF 164, 182, and 188) in pregnant bitches uterus as compared to non-pregnant females (P<0.001). Our results indicated that the expression of genes encoding integrins and vascular endothelial growth factors is different in relation to the time of the embryo implantation and it is increased in the first period of this process. This may be associated with the induction of specific mechanisms responsible for receptivity of uterus following the embryo attachment. In addition, all of investigated genes are up-regulated in a pregnancy-specific manner and the increased expression of these genes may regulate the uterus function during the implantation of canine embryos.
Źródło:
Polish Journal of Veterinary Sciences; 2011, 14, 2
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Interaction of three Caenorhabditis elegans isoforms of translation initiation factor eIF4E with mono- and trimethylated mRNA 5 cap analogues.
Autorzy:
Stachelska, Alicja
Wieczorek, Zbigniew
Ruszczyńska, Katarzyna
Stolarski, Ryszard
Pietrzak, Monika
Lamphear, Barry
Rhoads, Robert
Darżynkiewicz, Edward
Jankowska-Anyszka, Marzena
Powiązania:
https://bibliotekanauki.pl/articles/1043732.pdf
Data publikacji:
2002
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
fluorescence quenching
eIF4E
Caenorhabditis elegans
mRNA 5' cap
translation initiation
Opis:
Translation initiation factor eIF4E binds the m7G cap of eukaryotic mRNAs and mediates recruitment of mRNA to the ribosome during cap-dependent translation initiation. This event is the rate-limiting step of translation and a major target for translational control. In the nematode Caenorhabditis elegans, about 70% of genes express mRNAs with an unusual cap structure containing m32,2,7G, which is poorly recognized by mammalian eIF4E. C. elegans expresses five isoforms of eIF4E (IFE-1, IFE-2, etc.). Three of these (IFE-3, IFE-4 and IFE-5) were investigated by means of spectroscopy and structural modelling based on mouse eIF4E bound to m7GDP. Intrinsic fluorescence quenching of Trp residues in the IFEs by iodide ions indicated structural differences between the apo and m7G cap bound proteins. Fluorescence quenching by selected cap analogues showed that only IFE-5 forms specific complexes with both m7G- and m32,2,7G-containing caps (Kas 2×106 M-1 to 7×106 M-1) whereas IFE-3 and IFE-4 discriminated strongly in favor of m7G-containing caps. These spectroscopic results quantitatively confirm earlier qualitative data derived from affinity chromatography. The dependence of Kas on pH indicated optimal cap binding of IFE-3, IFE-4 and IFE-5 at pH 7.2, lower by 0.4 pH units than that of eIF4E from human erythrocytes. These results provide insight into the molecular mechanism of recognition of structurally different caps by the highly homologous IFEs.
Źródło:
Acta Biochimica Polonica; 2002, 49, 3; 671-682
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Wyświetlanie 1-15 z 39

Ta witryna wykorzystuje pliki cookies do przechowywania informacji na Twoim komputerze. Pliki cookies stosujemy w celu świadczenia usług na najwyższym poziomie, w tym w sposób dostosowany do indywidualnych potrzeb. Korzystanie z witryny bez zmiany ustawień dotyczących cookies oznacza, że będą one zamieszczane w Twoim komputerze. W każdym momencie możesz dokonać zmiany ustawień dotyczących cookies