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Tytuł:
Badania laboratoryjne przydatnosci biopreparatu Dipel w redukcji prusakow [Blattella germanica L.]
Laboratory studies of the usefulness of the biopreparation Dipel for redukction of the population of german cocroaches [Blattella germanica L.]
Autorzy:
Zukowski, K
Powiązania:
https://bibliotekanauki.pl/articles/873190.pdf
Data publikacji:
1993
Wydawca:
Narodowy Instytut Zdrowia Publicznego. Państwowy Zakład Higieny
Tematy:
ochrona srodowiska
zwalczanie szkodnikow
owady
biopreparaty
badania laboratoryjne
szkodniki zywnosci
prusak
Blattella germanica
Dipel
szkodniki magazynowe
karaczany
environment protection
pest control
insect
biopreparation
laboratory research
food pest
German cockroach
Blatella germanica
Dipel biopreparation
storage pest
cockroach
Opis:
Określono wrażliwość prusaków (Blattella germanica L.) różnorodnych pod względem wieku i płci, na owadobójczy biopreparat z Bacillus thuringiensis var. kurs taki (Dipel).
In the studies conducted for finding of biological factors effectively controlling insects of hygienic importance German cockroaches were subjected to the action of an insecticide containing spores of Bacillus thuringiensis var. kurstaki. One gram of the insecticide contained 25 miliard (US billion) spores with protein endotoxin crystals. The inscets were given drinking water and food containing 1.0%, 0.5%, 0.25%, 0.125% of the initial preparation. The experiment was carried out on 1800 cockroaches. Half of them (600 mature insects differentiated for sex and age and 300 larvae in stage 1st of development) were treated with the preparation, the remaining insects served for control. The insects were kept at about 28°C. After 9 days o the experiment in the group receiving 1% Dipel on food 76.4% mature insects and 83,0% larvae in 1st stage of development died. After 14 days all males died, and after 17 days died all females. In the control group died in that time 2.3% and 3.4% respectively. In case of lower per cent of the preparation in food the time of dying was prolonged, and at the lowest per cent it was 22 days. The study showed that Dipel effectively reduced the population of German cockroaches, and although in initial period its action was slower than that of chemical agents, it could be used for the control of these insects, especially as it in non-toxic for humans, warm-blooded animals and useful insects.
Źródło:
Roczniki Państwowego Zakładu Higieny; 1993, 44, 2-3; 227-230
0035-7715
Pojawia się w:
Roczniki Państwowego Zakładu Higieny
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Insecticidal efficacy of silica nanoparticles against Rhyzopertha dominica F. and Tribolium confusum Jacquelin du Val
Autorzy:
Ziaee, M.
Ganji, Z.
Powiązania:
https://bibliotekanauki.pl/articles/66106.pdf
Data publikacji:
2016
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
insecticidal potential
silica
nanoparticle
Rhyzopertha dominica
Tribolium confusum
barley
pest control
plant protection
wheat
insect pest
Opis:
Bioassays were conducted to assess the effects of two silicon dioxide nanoparticles of Aerosil® and Nanosav against adults of Rhyzopertha dominica F. and Tribolium confusum Jacquelin du Val. Silica nanoparticles were applied at the rates of 50, 100, 200 and 300 mg · kg–1 on wheat and peeled barley. The mortality was counted after 1, 2, 3, and 7 days of exposure. Another experiment was carried out to evaluate the effect of food source on the survival of beetles after exposure to silica nanoparticles. Adults were exposed to silica nanoparticles at the rate of 0.2 mg · cm–2 for 1 and 2 days on filter paper inside plastic Petri dishes, respectively. After exposure, the initial mortality was counted and live individuals of both species were held for a week in empty glass vials or vials containing wheat and wheat flour, respectively. Silica nanoparticles have high toxicity on R. dominica and T. confusum adults. Rhyzopertha dominica was more susceptible than T. confusum. However, the mortality of both species increased with increasing concentrations and time exposed to each concentration. At low concentrations, Aerosil® was more effective than Nanosav. Silica nanoparticles were more effective in wheat grains than barley. Results indicated that the initial mortality was so high that the impact of food source on delay mortality was unclear in most cases. Silica nanoparticles were efficient against tested species and can be used effectively in a stored grain integrated pest management program.
Źródło:
Journal of Plant Protection Research; 2016, 56, 3
1427-4345
Pojawia się w:
Journal of Plant Protection Research
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Development of thermotolerant isolates of Beauveria bassiana (Bals.-Criv.) Vuill. with ethyl methanesulfonate
Autorzy:
Wongwanich, Y.
Cobelli, P.
Boonchuay, D.
Wangsomboondee, T.
Powiązania:
https://bibliotekanauki.pl/articles/65516.pdf
Data publikacji:
2017
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
thermotolerance
entomopathogenic fungi
Beauveria bassiana
ethyl methanesulphonate
brown planthopper
Nilaparvata lugens
pest insect
pest control
mutation
fungal isolate
molecular identification
pathogenicity test
Opis:
Beauveria bassiana is an entomopathogenic fungus that is widely used in Thailand to control pest insects. However, the increasing temperature has influenced the insect control efficiency of the fungus. Therefore, determination of thermotolerant isolates of B. bassiana that can grow and remain pathogenic at higher temperatures than its current optimum temperature may be a better way to control pest insects in a high temperature environment. Three isolates of B. bassiana obtained from the Rice Department, Thailand were selected for mutagenesis using ethyl methanesulfonate (EMS) with subsequent screening at high temperatures (33 and 35°C). In addition, the recovery of fungal growth after exposure to a high temperature for a period of time (5–15 days) and then transferring to 25°C was evaluated. No isolates were found that grew at 35°C but one mutant isolate (BCNT002MT) produced larger diameter colonies and more spores than the corresponding wild type (WT) at 33°C. Growth and spore production of the BCNT002MT isolate were greater than its WT when incubated at 25°C for 14 days following exposure to 33°C for 7 days. In addition, the spore germination level (%) of BCNT002MT was significantly higher than its WT during culture at 25°C after prior exposure to 33°C for 5, 10 and 15 days. The pathogenicity against the brown planthopper, Nilaparvata lugens (Stål), of this mutant isolate was also prominent.
Źródło:
Journal of Plant Protection Research; 2017, 57, 4
1427-4345
Pojawia się w:
Journal of Plant Protection Research
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Activity of azadirachtin relating to Pieris brassicae and Apanteles glomeratus
Autorzy:
Wawrzyniak, M
Wrzesinska, D.
Powiązania:
https://bibliotekanauki.pl/articles/65381.pdf
Data publikacji:
2000
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
pest
cabbage plant
azadirachtin
Apanteles glomeratus
mite
insect
Azadirachta indica
Pieris brassicae
Opis:
Azadirachtin is the most active substance separated from Azadirachta indica. lt acts destructively on numerous species of insects and mites. The aim of the presented investigations was to determine the effect of azadirachtin on one of the most dangerous pests of cabbage plants, Pieris brassicae, and on its natural enemy, Apanteles glomeratus. The results of the field observations did not show any repellent effect of azadirachtin relating to laying their eggs cabbage butterflies. However, a significant effect of the azadirachtin solution on reduction the number of cabbage butterfly caterpillars (it was more distinctly marked in respect to young larva stages) as well as on limited feeding by caterpillars. No negative influence of azadirachtin on a parasite of Pieris brassicae caterpillars, Apanteles glomeratus was distinctly shown.
Azadirachtyna jest najbardziej aktywną substancją wyizolowaną z Azadirachta indica. Działa szkodliwie na liczne gatunki owadów i roztoczy. Celem przedstawionych doświadczeń było określenie jej wpływu na jednego z najgroźniejszych szkodników roślin kapustnych bielinka kapustnika (Pieris brassicae) oraz na jego wroga naturalnego baryłkarza bieliniaka (Apanteles glomeratus). Wyniki przeprowadzonych obserwacji polowych nie wykazały repelentnego działania azadirachtyny w stosunku do składających jaja motyli bielinka. Stwierdzono natomiast znaczący wpływ preparatu na redukcję liczby gąsienic bielinka kapustnika (wyraźniej zaznacza się to w odniesieniu do młodszych stadiów larwalnych) oraz na hamowanie pobierania pokarmu przez gąsienice. Nie wykazano wyraźnie negatywnego wpływu azadirachtyny na pasożyta gąsienic bielinka – baryłkarza bieliniaka.
Źródło:
Journal of Plant Protection Research; 2000, 40, 2
1427-4345
Pojawia się w:
Journal of Plant Protection Research
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
System for warning and control of leaf mining flies and leaf beetles on cereal crops
Autorzy:
Walczak, F
Powiązania:
https://bibliotekanauki.pl/articles/65294.pdf
Data publikacji:
1998
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
pest
Agromyzidae
crop damage
control
warning
larva
leaf mining fly
cereal crop
trapped insect
Opis:
Many species of leaf mining flies feed on cereal crops. Cereal crop plantations with high level of the pest infestation need to be treated with insecticides. The yellow tables covered with glue were used for trapping the pests. The leaf mining flies warning system can provide the information about date of treatment based on counting the trapped imagines of the pest and observation of leaf beetle appearance and its development. The following species of leaf mining flies are the most common in Poland: Phytomyza nigra Mg., Agromyza ambigua Fll., Ceroclontha pygmaea Mg., Agromyza mobilis Mg.
Wzrost liczebności i szkodliwości miniarek w uprawach zbóż w Polsce stwierdzono na początku lat dziewięćdziesiątych. Larwy miniarek żerują w tkance miękiszowej liści, w wyniku czego powstają charakterystyczne uszkodzenia (miny). W niektórych rejonach kraju lokalnie owady te mogą występować liczniej i przyczyniać się do znacznego ograniczenia powierzchni asymilacyjnej liści (głównie flagowych i podflagowych), co w konsekwencji ma wpływ na wartość i wielkość plonu. Celem pracy było opracowanie wskazówek metodycznych przy podejmowaniu decyzji o chemicznym zwalczaniu na zbożach miniarek równocześnie ze skrzypionkami. W wyniku przeprowadzonych badań stwierdzono, że dobrą praktyczną metodą sygnalizacji chemicznego zwalczania tych szkodników jest kontrola liczebności form dorosłych miniarek, odławianych na tablicach chwytnych w kolorze żółtym, przy równoległej obserwacji pojawu i rozwoju skrzypionek.
Źródło:
Journal of Plant Protection Research; 1998, 38, 1
1427-4345
Pojawia się w:
Journal of Plant Protection Research
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Wplyw zerowania przedziorkow [Acari: Tetranychidae] na zawartosc wybranych metabolitow wtornych na lisciach ogorka
Autorzy:
Tomczyk, A
Powiązania:
https://bibliotekanauki.pl/articles/800020.pdf
Data publikacji:
2006
Wydawca:
Szkoła Główna Gospodarstwa Wiejskiego w Warszawie. Wydawnictwo Szkoły Głównej Gospodarstwa Wiejskiego w Warszawie
Tematy:
ogorki
zwiazki fenolowe
metabolity wtorne
szkodniki roslin
odpornosc roslin
liscie
zerowanie
kukurbitacyna
przedziorki
odpornosc na szkodniki
cucumber
phenolic compound
secondary metabolite
plant pest
plant resistance
leaf
insect foraging
cucurbitacin
spider mite
Tetranychidae
pest resistance
Opis:
Porównywano zawartość związków fenolowych oraz kukurbitacyn w liściach roślin ogórka nieuszkodzonych i uszkodzonych przez przędziorka chmielowca (Tetranychus urticae Koch). Próby do analiz pobierano z liści uszkodzonych oraz z liści młodych, na których nie zaobserwowano jeszcze śladów żerowania szkodnika. W młodych liściach ogórka stwierdzono wyższą zawartość związków fenolowych, w porównaniu z liśćmi starszymi. W wyniku uszkodzenia roślin przez przędziorki poziom fenoli w młodych liściach tych roślin wzrósł w porównaniu z analogicznymi liśćmi roślin kontrolnych, co spowodowało dużą różnicę w zawartości fenoli między młodymi i dojrzałymi liśćmi roślin uszkodzonych. Analiza frakcji fenolowej przy użyciu HPLC wykazała zwiększoną zawartość kwasu salicylowego i kwasu chlorogenowego w młodych liściach uszkodzonych roślin, w porównaniu z analogicznymi liśćmi roślin kontrolnych. Zwiększona zawartość związków fenolowych, przede wszystkim kwasu salicylowego w młodych, jeszcze nieuszkodzonych liściach na uszkodzonej roślinie, wskazuje na indukcję odporności systemicznej w roślinach zaatakowanych przez przędziorki. W dojrzałych liściach, uszkodzonych przez przędziorki, stwierdzono wzrost zawartości kukurbitacyn, znanych jako metabolity wtórne, wpływające na żerowanie szkodników na roślinach dyniowatych. Terpenoidy te mogą być dla przędziorków inhibitorami żerowania. Zwiększenie zawartości kukurbitacyn w liściach ogórka, uszkodzonych przez przędziorki, może stanowić reakcję obronną roślin.
A higher level of total phenols was found in young cucumber leaves, as compared to the older ones. As the effect of mite infestation the amount of phenolic compounds increased in young leaves and was much higher than in the analogical leaves of the undamaged plants. Analysis of phenolic fraction with HPLC showed an increase of two phenolic acids: chlorogenic and salicylic in the young leaves of damaged plants as compared to the control. An increased level of phenolic compounds in the young leaves of damaged plants indicates the induction of systemic defense in plants attacked by spider mites. In many cases the increased level of phenolic compounds can also be observed in the damaged leaves but probably it depends on the level of leaf injury. The concentration of phenols usually increases at the beginning of the pest feeding and later it decreases. In damaged leaves, however, a higher concentration of cucurbitacins, which are known as the compounds affecting pest feeding activity on cucurbits, was detected. These terpenoids can be attractants for some pests in low concentrations and have a deterrent effect in higher concentrations on other insect or mite species. The increase in cucurbitacin concentration in the spider mite infested leaves can be defined as a plant defense response.
Źródło:
Zeszyty Problemowe Postępów Nauk Rolniczych; 2006, 509; 405-412
0084-5477
Pojawia się w:
Zeszyty Problemowe Postępów Nauk Rolniczych
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Ecotoxicological view of protection of apple orchards against insect pests in Poland
Autorzy:
Sadlo, S.
Szpyrka, E.
Powiązania:
https://bibliotekanauki.pl/articles/55453.pdf
Data publikacji:
2009
Wydawca:
Sieć Badawcza Łukasiewicz - Instytut Przemysłu Organicznego
Tematy:
tomato fruit
maximum residue limit
health risk
daily intake
human health
ecotoxicology
consumer exposure
pesticide
residue level
apple orchard
insect pest
quality index
apple fruit
plant protection
pesticide residue
Opis:
The paper presents some aspects of relations between modern plant protection of apple orchards and ecotoxicology. Based on significant similarity of the shape and size of apple and tomato fruits the relation between the doses of active ingredients of plant protection products and their residue levels immediately after treatment was derived for the apples and then quality indices and the risks for human health were estimated. It was found that many of the currently in force MRLs need to be revised and the exposure of consumer should not exceed the acceptable daily intakes (ADIs) and acute reference doses (ARfDs) for pesticide residues.
Źródło:
Pestycydy; 2009, 1-4; 15-26
0208-8703
Pojawia się w:
Pestycydy
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Toxicological and environmental aspects of carrot fly [Psila rosae Fabr.] control
Autorzy:
Sadlo, S.
Filar, M.
Szpyrka, E.
Powiązania:
https://bibliotekanauki.pl/articles/55198.pdf
Data publikacji:
2008
Wydawca:
Sieć Badawcza Łukasiewicz - Instytut Przemysłu Organicznego
Tematy:
insecticide
environment
carrot fly
residue
parsley
chlorothalonil
fungicide
toxicology
plantation
pest control
insect
plant protection
Psila rosae
chlorpyrifos
carrot
Opis:
The objectives of this work were to describe the residual behaviour of chlorpyrifos (insecticide) and chlorothalonil (fungicide), applied for the protection of carrot and parsley plantations. The field tests were carried out at farms located near Rzeszów (South-Eastern Poland) on processing carrot and parsley destined for baby food production. The results obtained indicated that disappearance rate of chlorothalonil on carrot leaves was significantly faster and its half-life time was about 7 times shorter than that of chlorpyrifos. In weather conditions of 2006, chlorpyrifos residues in rape carrot and parsley roots were reduced to levels lower than MRL and even the rigorous value of 0.01 mg/kg.
Źródło:
Pestycydy; 2008, 1-2; 119-126
0208-8703
Pojawia się w:
Pestycydy
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Możliwości wykorzystania Mamestra dissimilis Knoch. do regulacji zachwaszczenia przez Rumex confertus Willd.
Mamestra dissimilis Knoch. potential to control weed infestation with mossy sorrel (Rumex confertus Willd.)
Autorzy:
Piesik, D.
Wenda-Piesik, A.
Powiązania:
https://bibliotekanauki.pl/articles/47114.pdf
Data publikacji:
2005
Wydawca:
Politechnika Bydgoska im. Jana i Jędrzeja Śniadeckich. Wydawnictwo PB
Tematy:
Mamestra dissimilis
pietnowka zmienna
szkodniki roslin
zerowanie
Rumex confertus
szczaw omszony
chwasty
regulacja zachwaszczenia
biologiczne metody ochrony roslin
plant pest
insect foraging
weed
weed infestation control
biological plant protection
Opis:
Badania polowe i laboratoryjne prowadzono w celu oszacowania możliwości wykorzystania piętnówki zmiennej (Mamestra dissimilis Knoch., Lepidoptera: Noctuidae) do regulacji zachwaszczenia spowodowanego przez Rumex confertus (Willd.). W naturalnym siedlisku szczawiu badano dynamikę jego wzrostu oraz liczbę gąsienic piętnówki zmiennej zasiedlających roślinę w okresie 50 dni. Największą liczbę gąsienic odłowiono pod koniec pierwszej dekady czerwca, w czasie gdy roślina żywicielska miała już w pełni wykształconą rozetę. W warunkach laboratoryjnych badano wzrost masy ciała gąsienic oraz ich żerowanie w temperaturze 20oC. Zależności pomiędzy masą zjedzonych liści szczawiu omszonego a przyrostami masy larw L4 i L5 były statystycznie istotne (r = 0,97 i r = 0,88). Stwierdzono bardzo progresywny charakter żerowania larw L4, opisany równaniem 1. stopnia (r = 0,98). Wyliczono, że każdego dnia w ciągu 20 dni żerowania larwy zjadły o 5,9 mg więcej liści. Natomiast dla gąsienic L5 związek ten miał charakter funkcji 2. stopnia (r = 0,96). Oszacowana masa liści szczawiu omszonego, która uległa skonsumowaniu przez larwy piętnówki zmiennej w warunkach naturalnych, wyniosła 159,3 g, co stanowiło około 11% utraty biomasy żywiciela.
Field and laboratory research were conducted to determine the potential of Mamestra dissimilis Knoch., Lepidoptera: Noctuidae to control mossy sorrel (Rumex confertus Willd.). In the natural sorrel habitat a dynamics of the plant growth and the number of Mamestra dissimilis Knoch larvae occurring on that plant over 50 days were researched. The highest number of M. dissimilis larvae was caught at the end of the first decade of June when the host plant rosette was fully developed. In the laboratory, the weight of consumed food by larvae, and larval body weight were measured at 20oC. The relations between the weight of the mossy sorrel leaves consumed and L4 and L5 larvae body weight gains were significant, (r = 0.97 and r = 0.88, respectively). A very progressive feeding of L4 larvae was noted, which was defined by first degree equation (r = 0.98). It was calculated that each day over 20 days of feeding L4 larvae consumed about 5.9 mg more leaves than L5 larvae. For L5 larvae, the relationship was the second degree equation (r = 0.96). An estimated weight of mossy sorrel leaves consumed by M. dissimilis larvae under natural conditions was 159.3 g, which accounted for about 11% loss of the host biomass.
Źródło:
Acta Scientiarum Polonorum. Agricultura; 2005, 04, 1
1644-0625
Pojawia się w:
Acta Scientiarum Polonorum. Agricultura
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Rural farmers’ perceptions, knowledge and management of insect pests of fruit vegetables in Ogbomoso Agricultural Zone of Nigeria
Autorzy:
Olaniran, O.A.
Babarinde, S.A.
Odewole, A.F.
Aremu, P.A.
Popoola, K.
Powiązania:
https://bibliotekanauki.pl/articles/10930.pdf
Data publikacji:
2014
Wydawca:
Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
Tematy:
rural area
farmer
perception
knowledge
management
insect pest management
beetle
vegetable-like fruit
Ogbomoso Agricultural Zone
Nigeria
Opis:
Surveys were carried out in five local government areas of Ogbomoso Agricultural Zone Nigeria during planting season in 2010 to investigate rural farmers perceptions and management practices of insect pests of fruit vegetables. The survey involved 150 randomly selected farmers who were interviewed using structured questionnaire. Fruit vegetables planted by the farmers were okra, tomatoes, pepper and garden egg. Reasons given by farmers for cultivating fruit vegetables were significantly favored by age category, educational qualification, and secondary occupation of farmers (x2 = 4.757, P = 0.029). Field insect pests were perceived as the major production constraint to fruit vegetables in this zone. Majority of the farmer estimated 78.4 % rated insect pest as the most serious pest infesting fruit vegetables, the insect pests were grasshopper (Orthoptera), beetles (Coleoptera) and caterpillar (Lepidoptera). Integrated pest management module consisted basically of chemical and cultural control strategies, with weeding, shifting cultivation and crop rotation as main cultural control methods. More than 76.7 % of the farmers make use of cultural control because of unavailability and cost of chemical insecticides. Only 23.3 % had access to chemical insecticides in controlling insect pest of fruit vegetables.
Źródło:
International Letters of Natural Sciences; 2014, 20
2300-9675
Pojawia się w:
International Letters of Natural Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Comparative efficacy of plant extracts with synthetic against insect pests of okra (Abelmoschus esculentus L. Monech)
Autorzy:
Odewole, A.F.
Alao, F.O.
Agboola, A.A.
Adedapo, B.F.
Powiązania:
https://bibliotekanauki.pl/articles/11813.pdf
Data publikacji:
2014
Wydawca:
Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
Tematy:
comparative efficacy
plant extract
insect pest
okra
Abelmoschus esculentus
insecticide
Podagrica uniformis
Podagrica sjostedti
Zenocerus variegatus
Opis:
A field study was carried out during the two cropping seasons of okra to investigate the efficacy of plant extracts and synthetic insecticides applied singly and synergistic effect of botanicals with the synthetic insecticide on some insect pests of okra. Plots were arranged in a Randomized Complete Block Design (RCBD), with three replications. Three insect pests of okra (Podagrica uniformis, Podagrica sjostedti and Zenocerous variegatus) were observed on the field. Cypermethrin applied singly and Cypermethrin combined with plant extracts effectively controlled observed insect pests, followed by lone application of plant extracts (L camara, T. vogelii and Z. officinales plant extracts at 1st and 2nd season. Okra yield revealed that both plant extract and Cypermethrin combined with plant extract were not significantly different from one another at first season and at second season Cypermethrin combined with plant extracts performed better than Cypermethrin applied singly. This study indicates that the studied plant extracts can be good synergist for Cypermethrin in controlling field pest of okra.
Źródło:
International Letters of Natural Sciences; 2014, 14
2300-9675
Pojawia się w:
International Letters of Natural Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Insecticidal activity of three 10–12 nucleotides long antisense sequences from 5.8S ribosomal RNA gene of gypsy moth Lymantria dispar L. against its larvae
Autorzy:
Oberemok, V.V.
Laikova, K.V.
Useinov, R.Z.
Gal`chinsky, N.V.
Novikov, I.A.
Yurchenko, K.A.
Volkov, M.E.
Gorlov, M.V.
Brailko, V.A.
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Powiązania:
https://bibliotekanauki.pl/articles/2084849.pdf
Data publikacji:
2019
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
antisense oligonucleotides
DNA insecticides
gypsy moth
insect pest control
Lymantria dispar
5.8S ribosomal RNA
Opis:
5.8S ribosomal RNA plays an important role in protein synthesis and eukaryotic ribosome translocation. Contact DNA insecticides based on antisense fragments of 5.8S ribosomal RNA gene of gypsy moth Lymantria dispar L. showed prospective insecticidal activity on its larvae. The most pronounced insecticidal effect was found for antisense fragments 10 and 11 nucleotides long (oligoRIBO-10 and oligoRIBO-11), whereas 12 nucleotides long fragment (oligoRIBO-12) caused the lowest level of insect mortality. This data corresponds to results obtained earlier using rabbit reticulocyte and wheat germ extracts, where maximum inhibition of protein synthesis was observed when a relevant oligomer 10-11 nucleotides long was used, whilst longer chain lengths resulted in reduced inhibition. Using oligoRIBO-11 fragment we have shown penetration of antisense oligonucleotides to insect cells through insects’ exoskeletons. MALDI technique registered the penetration of the oligoRIBO-11 fragment into insect cells after 30 min and a significant response of insect cells to the applied oligonucleotide after 60 min, which indicates not only that the oligonucleotide enters the insect cells, but also the synthesis of new substances in response to the applied DNA fragment. Contact DNA insecticides developed from the L. dispar 5.8S ribosomal RNA gene provide a novel biotechnology for plant protection using unmodified antisense oligonucleotides.
Źródło:
Journal of Plant Protection Research; 2019, 59, 4; 561-564
1427-4345
Pojawia się w:
Journal of Plant Protection Research
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The need for the application of modern chemical insecticides and environmental consequences of their use: a mini review
Autorzy:
Oberemok, V.V.
Laikova, K.V.
Zaitsev, A.S.
Temirova, Z.Z.
Gal'chinsky, N.V.
Nyadar, P.M.
Shumskykh, M.N.
Zubarev, I.V.
Powiązania:
https://bibliotekanauki.pl/articles/66108.pdf
Data publikacji:
2017
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
application need
modern insecticide
insecticide
chemical insecticide
environmental consequence
DNA insecticide
insect pest
insecticide resistance
plant protection
Opis:
Currently, the use of insecticides is an acute problem. Due to rapid population growth, the primary task is to increase food production. Beyond abiotic factors (drought, soil salinity, etc.) that reduce crop yields, farmers face problems with insect pests that can decrease crop productivity up to 60%. Also, insects are carriers of severe viral and protozoan human diseases. The need for application of insecticides is not questioned but many of them cause resistance of insect pests to them. This, in turn, leads to the necessity to invent new insecticides that are safe and more effective for long-term use. Preparations based on conservative parts of nucleic acids, particularly contact DNA insecticides, could be used to solve insecticide resistance problem as control agents which are well-tailored to target insect pests. This mini review is devoted to these issues.
Źródło:
Journal of Plant Protection Research; 2017, 57, 4
1427-4345
Pojawia się w:
Journal of Plant Protection Research
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Morphological and molecular characterization of entomophthorales (Entomophthoromycota: Entomophthoromycotina) from Argentina
Autorzy:
Manfrino, Romina G.
Castrillo, Louela A.
López Lastra, Claudia C.
Toledo, Andrea V.
Ferrari, Walter
Jansen, Annette B.
Powiązania:
https://bibliotekanauki.pl/articles/11543394.pdf
Data publikacji:
2020
Wydawca:
Polskie Towarzystwo Botaniczne
Tematy:
morphological characteristics
molecular characteristics
Entomophthorales
Entomophthoromycota
Entomophthoromycotina
insect pest
host range
Argentina
Opis:
We characterized 17 insect-pathogenic entomophthoralean fungal isolates (Entomophthoromycotina: Entomophthorales) using morphological and molecular techniques. We identified four species from various insect hosts: (i) Entomophthora planchoniana, six specimens from aphids; (ii) Pandora neoaphidis, three specimens from aphids; (iii) Zoophthora phalloides from an aphid; and (iv) Z. radicans, seven specimens from insects in the orders Diptera, Hemiptera, and Lepidoptera. Analysis of ITS1 data from E. planchoniana showed clustering in accordance to aphid host species. Entomophthora planchoniana from Macrosiphum euphorbiae clustered together, separate from the isolate from Myzus persicae. The P. neoaphidis specimens clustered with sequences from other aphid-pathogenic Pandora species in GenBank. In this study, Z. phalloides from Brevicoryne brassicae and Z. radicans from an unidentified species of Chironomidae (Diptera) in Argentina were characterized for the first time. The present study was initiated to elucidate the taxonomy of the entomophthoralean fungi in Argentina according to their morphological and molecular characters. The presented results emphasize the significance of the combination of molecular data and information on morphology, ecology, and host range for accurate identification of entomophthoralean and allied genera.
Źródło:
Acta Mycologica; 2020, 55, 2; 5522
0001-625X
2353-074X
Pojawia się w:
Acta Mycologica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
An appraisal of insect pests associated with cereal grains traded in Maiduguri, Borno State, Nigeria
Autorzy:
Mailafiya, D.M.
Dauda, Z.
Degri, M.M.
Kabir, B.G.J.
Maina, Y.T.
Powiązania:
https://bibliotekanauki.pl/articles/11837.pdf
Data publikacji:
2014
Wydawca:
Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
Tematy:
insect pest
stored product
cereal grain
infestation
insect abundance
grain type
Maiduguri town
Borno State
Nigeria
Opis:
This study investigated insect pest infestation of maize, millet, sorghum, rice and wheat grains traded in the three main markets namely Monday, Baga-Road and Custom-Park markets in Maiduguri, Borno state, Nigeria. Sampling lasted from January to June 2012. Samples per grain type, each weighing one kilogram, were obtained every two weeks from ten randomly selected traders per market. Each sample was sieved in the laboratory to recover all adult insects present, both at sampling and also after one month stay (laid out in a completely randomized design). In each market, Sitophilus zeamais Motschulsky and Rhyzopertha dominica (F.) followed by Tribolium castaneum Herbst, Sitophilus oryzae (Linnaeus), Trogoderma granarium (Everts) or Cryptolestes sp. were the insect pests commonly found attacking more (between two to five) grain types. Observed highest and lowest mean insect numbers were respectively by S. zeamais in maize grains (12.13 individuals in Monday market) and T. granarium in wheat grains (0.13 individuals in Baga-Road market). Results unveil that among three markets, higher mean total numbers of insects were present in maize, rice or wheat (18-33). For all grain types, the mean numbers of damaged grains were significantly higher in Monday (53-357) and Custom-Park (46-302) than in Baga-Road (17-109) markets. Furthermore, the mean total number of damaged grains across markets was significantly higher maize, followed by rice and wheat (55-231) than in other grain types (37-41). The implications of all these results on insect infestation of different cereal grain types sold in these markets are discussed.
Źródło:
International Letters of Natural Sciences; 2014, 14
2300-9675
Pojawia się w:
International Letters of Natural Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł

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