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    Wyświetlanie 1-29 z 29
    Tytuł:
    Effect of post-thaw supplementation of fractionated seminal plasma on survival of boar spermatozoa
    Autorzy:
    Wasilewska-Sakowska, K.
    Zasiadczyk, Ł.
    Fraser, L.
    Strzeżek, J.
    Karpiesiuk, K.
    Powiązania:
    https://bibliotekanauki.pl/articles/2087478.pdf
    Data publikacji:
    2019
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    boar
    semen
    fractionated seminal plasma
    cryopreservation
    Źródło:
    Polish Journal of Veterinary Sciences; 2019, 3; 617-625
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    The cryoprotective potential of propolis supplemented in frozen-thawed bull semen; biochemical and physiological findings
    Autorzy:
    Yeni, D.
    Gülhan, M.F.
    İnanç, M.E.
    Avdatek, F.
    Güngör, Ş.
    Türkmen, R.
    Tuncer, P.B.
    Taşdemir, U.
    Powiązania:
    https://bibliotekanauki.pl/articles/16539069.pdf
    Data publikacji:
    2022
    Wydawca:
    Polska Akademia Nauk. Czasopisma i Monografie PAN
    Tematy:
    cryopreservation
    DNA damage
    oxidative stress
    propolis
    Opis:
    In this study, the cryoprotective effect of different doses of propolis (P) on bull semen, which has solid pharmacological properties thanks to its rich phenolic components, was investigated biochemically and physiologically. Semen samples were collected from Simmental breed bulls via the artificial vagina and pooled. After dividing into five groups, control (C: no additive) and four different P (200, 100, 50, and 25 μg/mL) groups, the final concentration was diluted to 16×106 per straw. Semen samples were equilibrated at 4°C for approximately 4 hours, then placed in French straws and frozen. After thawing, sperm motility and kinetic parameters, DNA integrity by single-cell gel electrophoresis, sperm abnormalities by liquid fixation, and lipid peroxidation levels by the colorimetric method was analyzed by Computer-Assisted Semen Analyzer. P added to the diluent showed no effect on motility and kinetic parameters at P25 and P50 (p>0.05), while P100 and P200 had a negative effect (p<0.001). The addition of P (25 and 50) showed a treatment effect on tail abnormality compared to C (p<0.05). Especially P50 had a positive effect on tail length, tail DNA, and tail movement, while P100 and P200 caused DNA damage (p<0.001). MDA levels increased in all P dose groups compared to C (p<0.001). This study has clearly demonstrated that P25 and P50 supplements could be used therapeutically to treat sperm tail abnormalities and prevent DNA damage in post-thawed bull sperm.
    Źródło:
    Polish Journal of Veterinary Sciences; 2022, 25, 1; 5-12
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    The effect of luteolin on spermatological parameters, apoptosis, oxidative stress rate in freezing rabbit semen
    Autorzy:
    Akarsu, S.A.
    Acısu, T.C.
    Güngör, İ.H.
    Çakır Cihangiroğlu, A.
    Koca, R.H.
    Türk, G.
    Sönmez, M.
    Gür, S.
    Fırat, F.
    Esmer Duruel, H.E.
    Powiązania:
    https://bibliotekanauki.pl/articles/16647483.pdf
    Data publikacji:
    2023
    Wydawca:
    Polska Akademia Nauk. Czasopisma i Monografie PAN
    Tematy:
    rabbit
    semen
    cryopreservation
    luteolin
    oxidative stress
    apoptosis
    Opis:
    The aim of the present study was to determine the effects of Luteolin (LUT) on semen quality, oxidative stress, apoptosis, acrosomal integrity, mitochondrial membrane potential and dead sperm ratio in rabbits. Ejaculates from six New Zealand rabbits were collected, evaluated and pooled. The pooling was divided into five groups as control (no additive) LUT 25 μM, LUT 50 μM, LUT 100 μM and LUT 200 μM and LUT added. It was then filled into a falcon tube with Tris-based extender at a final concentration of approximately 35 x 106 spermatozoa. Diluated rabbit semen samples were drawn into frozen and thawed. Frozen semen straws were thawed at 37°C in 30 seconds. According to our findings, no statistical difference was found between all doses of luteolin and the control group in the CASA (computer assisted sperm analysis) analysis performed at 4°C. However, total motility, progressive motility and rapid sperm percentage were found to be higher in the frozen and thawed rabbit semen at a dose of LUT 50 μM compared to the other groups (p<0.05). While amplitude of lateral head displacement (ALH) and beat cross-frequency (BCF) values were found at the lowest dose of LUT 200 μM, a statistically significant difference was observed between the other groups. When the flow cytometry results were examined, no statistical difference was found between the rate of dead sperm, acrosomal integrity, mitochondrial membrane potential and apoptosis rate. Morever, the H2O2 percentage was found to be lower in all experimental groups compared to the control group (p<0.001). In conclusion, the addition of LUT in long-term storage of rabbit semen provided a protective effect for spermatozoa with its antioxidative properties against damage caused by cryopreservation.
    Źródło:
    Polish Journal of Veterinary Sciences; 2023, 26, 1; 91-98
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Freezability and fertility of frozen-thawed boar semen supplemented with ostrich egg yolk lipoproteins
    Autorzy:
    Fraser, L.
    Zasiadczyk, Ł.
    Strzeżek, J.
    Strzeżek, R.
    Karpiesiuk, K.
    Powiązania:
    https://bibliotekanauki.pl/articles/2087726.pdf
    Data publikacji:
    2018
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    boar
    egg yolk lipoproteins
    cryopreservation
    artificial insemination
    Źródło:
    Polish Journal of Veterinary Sciences; 2018, 21, 2; 255-263
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Interactions of ostrich egg yolk lipoproteins with seminal plasma of fractionated ejaculates and their effects on post-thaw boar semen quality
    Autorzy:
    Fraser, L.
    Zasiadczyk, Ł.
    Strzeżek, J.
    Kordan, W.
    Mańkowska, A.
    Powiązania:
    https://bibliotekanauki.pl/articles/2087484.pdf
    Data publikacji:
    2019
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    boar semen
    ejaculate fractions
    egg yolk lipoproteins
    cryopreservation
    Źródło:
    Polish Journal of Veterinary Sciences; 2019, 3; 463-473
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Quality parameters and fertility of ram semen cryopreserved in egg yolk and soybean lecithin supplemented extenders
    Autorzy:
    Gogol, P.
    Bryła, M.
    Trzcińska, M.
    Bochenek, M.
    Powiązania:
    https://bibliotekanauki.pl/articles/2087594.pdf
    Data publikacji:
    2019
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    ram semen
    cryopreservation
    soybean lecithin
    sperm quality
    fertility
    Źródło:
    Polish Journal of Veterinary Sciences; 2019, 1; 177-179
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Effects of spermidine on germination of Salix spp. after storage under controlled conditions
    Autorzy:
    Wawrzyniak, M.K.
    Ley-Lopez, J.M.
    Kijowska-Oberc, J.
    Chmielarz, P.
    Ratajczak, E.
    Powiązania:
    https://bibliotekanauki.pl/articles/2130180.pdf
    Data publikacji:
    2022
    Wydawca:
    Polska Akademia Nauk. Instytut Dendrologii PAN
    Tematy:
    hydrogen peroxide
    cryopreservation
    spermidine
    Salix
    antioxidant
    seed storage
    Opis:
    Willows produce fast germinating and short-lived seeds, difficult to store in the long-term under controlled conditions. The aim of this study was to examine the feasibility of storage of three Salix spp. at controlled temperatures (3°, −10°, −196 °C). We also analyzed the effect of spermidine (Spd) as an antioxidant factor in desiccated seeds. Collected seeds were either desiccated or hydrated to obtain 10 levels of moisture content (between app. 4% and 2%) and subjected to storage at temperatures 3°, −10°, or −196 °C (liquid nitrogen; LN). After two months, seeds were germinated on the light at 20 °C. Seeds desiccated below a safe range of moisture content were further tested and germinated on filter paper with additions of 0.25 mM Spd solution. After 7 days seedlings were examined for hydrogen peroxide content (H2O2) and total antioxidant capacity (TAC). Fresh seeds of three Salix species: Persian willow (S. aegyptiaca L.), heartleaf willow (S. cordata Michx.) and crack willow (S. ×fragilis L.) were successfully stored at temperature −10° and −196 °C for two months. After cryopreservation seed of S. aegyptiaca, S. cordata, and S. ×fragilis germinated without viability loss in moisture content ranging from 4.4–15.9%, 6.4–18.5%, and 7.1–11.5% respectively. The addition of Spd during germination of desiccated seed did not affect germination capacity. However, seedlings of S. aegyptiaca had lower hydrogen peroxide content in comparison with control (germination on water). Seedlings of S. cordata showed an increase in hydrogen peroxide content in control after storing in LN. In seedlings of Crack willow Spd increased hydrogen peroxide content. Seeds of tested species differ in response to storage conditions. Salix seeds can be stored successfully for two months at −10° or −196 °C without losing viability in the safe range of moisture content. Storing at 3 °C can be used for storage in the narrower range of seeds’ moisture content, however, seedlings stored at this temperature produce a higher level of reactive oxygen species. Germinating seeds in Spd did not increase their germination, however in S. aegyptiaca and S. cordata decreased hydrogen peroxide content.
    Źródło:
    Dendrobiology; 2022, 87; 137-148
    1641-1307
    Pojawia się w:
    Dendrobiology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Changes in cell physiology at the stage of dehydration in a cryopreservation protocol
    Autorzy:
    Domzalska, L.
    Kedracka-Krok, S.
    Mikula, A.
    Rybczynski, J.J.
    Powiązania:
    https://bibliotekanauki.pl/articles/951293.pdf
    Data publikacji:
    2015
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    cryopreservation
    cell physiology
    dehydration
    Gentiana cruciata
    proteomic analysis
    Źródło:
    BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2015, 96, 1
    0860-7796
    Pojawia się w:
    BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Importance of explant size and origin for cryopreservation of Rosa pomifera “Karpatia” shoot tips by a droplet vitrification method
    Autorzy:
    Kwasniewska, E.
    Dziedzic, E.
    Pawlowska, B.
    Powiązania:
    https://bibliotekanauki.pl/articles/951284.pdf
    Data publikacji:
    2015
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    cryopreservation
    Rosa pomifera
    shoot tip
    droplet vitrification
    axenic culture
    Źródło:
    BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2015, 96, 1
    0860-7796
    Pojawia się w:
    BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Phytophthora Infestans: Isolation of Pure Cultures, Storage and Inoculum Preparation
    Autorzy:
    Sobkowiak, Sylwester
    Śliwka, Jadwiga
    Powiązania:
    https://bibliotekanauki.pl/articles/2199698.pdf
    Data publikacji:
    2017-12-20
    Wydawca:
    Instytut Hodowli i Aklimatyzacji Roślin
    Tematy:
    cryopreservation
    isolate
    late blight
    potato
    rye B agar medium
    Opis:
    Phytophthora infestans causes potato and tomato late blight, economically the most important disease of these plant species. The Oomycete pathogen is frequently sampled, isolated to pure cultures, stored, and char-acterized. The knowledge of its diversity, migrations and evolution is essential for breeding resistant plants and for designing appropriate control strategies. The article presents methods for collection, storage and prep-aration of P. infestans isolates for inoculation of plant tissues, based on the publication by Zarzycka (2001), later updated and modified.
    Źródło:
    Plant Breeding and Seed Science; 2017, 76; 9-15
    1429-3862
    2083-599X
    Pojawia się w:
    Plant Breeding and Seed Science
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Bioetyczny i teologicznomoralny wymiar zamrażania ludzkich embrionów
    The Bioethical and Moral Theology Dimension of Human Embryo Cryopreservation
    Autorzy:
    Wróbel, Józef
    Powiązania:
    https://bibliotekanauki.pl/articles/2035013.pdf
    Data publikacji:
    2020-03-30
    Wydawca:
    Katolicki Uniwersytet Lubelski Jana Pawła II. Towarzystwo Naukowe KUL
    Tematy:
    zamrażanie embrionów
    ART
    prokreacja wspomagana
    cryopreservation of embryos
    assisted procreation
    Opis:
    Artykuł jest poświęcony problematyce kriokonserwacji ludzkich embrionów. Stosowanie tej techniki pozostaje w ścisłym związku z prokreacją wspomaganą. Stwarzane celem zwiększenia skuteczności procedury in vitro nadliczbowe embriony zostają zamrożone, aby następnie można było je wykorzystać w kolejnych próbach transferu. Autor przedstawia techniczny wymiar zamrażania i ryzyko z nim związane. W ostatniej części Autor analizuje wymiar etyczny tych technik. Podkreśla, że chociaż sama prokreacja wspomagana jest niemoralna, to jednak moralnie słuszne jest dążenie do naprawienia tego zła przez matkę, która jest gotowa przyjąć do swojego łona stopniowo rozmrażane embriony. Dobrym rozwiązaniem wydaje się być adopcja porzuconych embrionów. Takiego rozwiązania nie da się jednak usprawiedliwić z punktu widzenia etycznego. Jeżeli rozmrożenie zostaje wymuszone przez prawo lub wymówienie umowy przez klinikę przechowywującą te embriony, możliwa jest zgoda na ich obumarcie. Z obowiązku transferu zwalnia również stan zdrowotny embrionów uniemożliwiający ich rozwój. Ponadto niemoralna jest selekcja eugeniczna embrionów oraz transfer wyłącznie tych embrionów, które mają pożądane cechy fenotypowe czy płeć.
    The article discusses the issues of cryopreservation of human embryos. The use of this technique is related to assisted procreation. The supernumerary embryos created in order to increase the effectiveness of the in vitro procedure are frozen so that they can be used in subsequent transfer attempts. The author presents the technical dimension of freezing and the risks associated with it. In the last part, the author analyzes the ethical dimension of these techniques. He emphasize that although assisted procreation is immoral itself, it is morally right to strive to correct this evil by the mother, who is ready to accept into her womb the thawed embryos. The adoption of abandoned embryos seems to be a good solution. However, this solution cannot be justified from the ethical point of view. If it is forced by law to defrost or terminate the contract by the clinic that stores the embryos, it is possible to consent to their death. The health condition of the embryos, which makes their development impossible, releases from the obligation to transfer. Moreover, it is immoral to select embryos by eugenics and transfer only those embryos that have the desired phenotypic or sex characteristics.
    Źródło:
    Roczniki Teologiczne; 2020, 67, 3; 75-103
    2353-7272
    Pojawia się w:
    Roczniki Teologiczne
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    The quality and fertilizing ability of the ram semen cryopreserved around 40 years ago
    Autorzy:
    Gogol, P.
    Powiązania:
    https://bibliotekanauki.pl/articles/16647392.pdf
    Data publikacji:
    2022
    Wydawca:
    Polska Akademia Nauk. Czasopisma i Monografie PAN
    Tematy:
    ram semen
    ex situ storage
    gene banks
    cryopreservation
    sperm quality
    fertility
    Opis:
    The aim of the study was to verify the quality of ram semen, frozen in 1982-1983, from the historical collection of the Bank of Biological Material of the National Research Institute of Animal Production. A total of 18 ejaculates from 3 Świniarka type rams were analyzed to assess sperm motility (subjectively), total motility, progressive motility, sperm concentration (CASA), membrane integrity (SYBR-14/PI) and chromatin structure (SCSA). In order to determine sperm fertilizing ability 49 ewes were intracervically inseminated (200×106 sperm per AI) with frozen- thawed semen 12 and 24 hours after detection of estrus. Sperm motility parameters, membrane intact spermatozoa and DFI did not differ among the analyzed rams. Spermatozoa concentration was significantly higher for ram no. 2 than for rams no. 1 and 3. The lambing rates (27.3 to 36.0%) did not differ significantly for individual rams. The ram semen, which had been stored for around 40 years, showed satisfactory quality and fertilizing capacity, allowing for its use in artificial insemination.
    Źródło:
    Polish Journal of Veterinary Sciences; 2022, 25, 4; 631-633
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    A simple way to overcome the recalcitrance of the water fern Ceratopteris thalictroides (L.) Brongn. to cryopreservation
    Autorzy:
    Makowski, D.
    Rybczynski, J.J.
    Mikula, A.
    Powiązania:
    https://bibliotekanauki.pl/articles/57732.pdf
    Data publikacji:
    2015
    Wydawca:
    Polskie Towarzystwo Botaniczne
    Tematy:
    abscisic acid
    water fern
    fern
    Ceratopteris thalictroides
    cryopreservation
    encapsulation-dehydration method
    darkness
    Opis:
    Ceratopteris thalictroides is a water fern very sensitive to both dehydration and low temperature. This study focuses on the cryopreservation of this species by encapsulation-dehydration technique, in particular on the effects of pre-culture step, alginate bead size and the physical conditions of culture on the cryopreservation efficiency. Encapsulated and non-pre-cultured gametophytes did not survive cooling with liquid nitrogen. When cryopreservation was preceded by a 2-week period of pre-culture, regrowth reached 42.1%. Reduction in the size of the alginate bead, and culture in total darkness resulted in improved gametophyte regrowth capacity (75.5% or 81.7%, respectively). The best results (91.3%) were obtained when all factors tested occurred simultaneously. The gametophytes recovered very quickly and sporophytes were formed within 4 weeks after rewarming. These simple improvements can be used, not only for the cryopreservation of gametophytes in cryptogams but also for some recalcitrant species of seed plants.
    Źródło:
    Acta Societatis Botanicorum Poloniae; 2015, 84, 3
    0001-6977
    2083-9480
    Pojawia się w:
    Acta Societatis Botanicorum Poloniae
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Sensitivity of Tilia cordata seeds to dehydration and temperature of liquid nitrogen
    Autorzy:
    Chmielarz, P
    Powiązania:
    https://bibliotekanauki.pl/articles/41625.pdf
    Data publikacji:
    2002
    Wydawca:
    Polska Akademia Nauk. Instytut Dendrologii PAN
    Tematy:
    dehydration
    drying
    liquid nitrogen
    temperature
    Tilia cordata
    forest habitat
    sensitivity
    scarification
    cryopreservation
    seed
    Opis:
    The aim of the study was to assess the susceptibility of small-leaved lime (Tilia cordata Mill.) seeds to drying and freezing in liquid nitrogen (-196°C). Seed samples were frozen in liquid nitrogen for 24 h at 11 different levels of seed moisture content (m.c.), ranging from 3.1% to 22.8% (fresh weight basis). All samples, including unfrozen control samples, were subjected to scarification with concentrated sulphuric acid (Tylkowski 1998) either before or after freezing. Seed pre-treatment before germination (at 3~15°C/16~8h) involved cold stratification at 3°C without substrate. Seed drying to 3.1% m.c. significantly reduced their germinability (to 63%), as compared to the high germinability (82-88%) of seeds with 5.2-20.9% m.c. Thus seeds of this species can be assigned to the ‘suborthodox' category. Such a high germinability (79-87%) was preserved after freezing in liquid nitrogen in samples dried to 9.0-17.4% m.c. if scarification was performed before freezing, and in samples dried to 9.1-16.2% m.c. if scarification was performed after freezing. The highest percentage of seedlings emerged after freezing in liquid nitrogen from seeds dried to 11.1-20.1% m.c. (emergence 65-75%) if scarification was performed before freezing, and from seeds dried to 7.3-17.8% m.c. (emergence 53%-71%) if scarification was performed after freezing.
    Źródło:
    Dendrobiology; 2002, 47 Supplement
    1641-1307
    Pojawia się w:
    Dendrobiology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Changes in DNA methylation of embryonic axes of seeds of oak and beech during in vitro culture and cryopreservation
    Autorzy:
    Nuc, K.
    Marszalek, M.
    Pukacki, P.M.
    Powiązania:
    https://bibliotekanauki.pl/articles/80269.pdf
    Data publikacji:
    2013
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    conference
    DNA methylation
    plant growing
    embryonic axis
    seed
    oak
    beech
    in vitro culture
    cryopreservation
    Źródło:
    BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2013, 94, 3
    0860-7796
    Pojawia się w:
    BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Zagrożenia podczas krioprezerwacji osi zarodkowych nasion drzew
    Threats during cryopreservation of seed embryonic axes of woody plants
    Autorzy:
    Juszczyk, K.
    Pukacki, P.M.
    Powiązania:
    https://bibliotekanauki.pl/articles/994516.pdf
    Data publikacji:
    2013
    Wydawca:
    Polskie Towarzystwo Leśne
    Tematy:
    lesnictwo
    nasiennictwo
    drzewa lesne
    nasiona
    kriokonserwacja
    osie zarodkowe
    stres oksydacyjny
    cryopreservation
    embryo axes
    oxidative stress
    Opis:
    Cryopreservation in liquid nitrogen (–196℃, LN) is the method of long−term conservation of plant tissues, which has been evolving for many of seed of woody plant species. During cryopreservation protocol to LN in plant cells take place the supercooling tissue water or vitrification, which allows the successful storage. Water content was a significant determining factor with survival of cryostored embryonic axes of woody plants. The protocols of cryopreservation include some steps during preparation of plant material, which could be a source of oxidative stress, phase transition of membranes and destruction in cells.
    Źródło:
    Sylwan; 2013, 157, 11; 842-853
    0039-7660
    Pojawia się w:
    Sylwan
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Effect of platelet activating factor (PAF) supplementation in semen extender on viability and ATP content of cryopreserved canine spermatozoa
    Autorzy:
    Kordan, W.
    Lecewicz, M.
    Strzezek, R.
    Dziekonska, A.
    Fraser, L.
    Powiązania:
    https://bibliotekanauki.pl/articles/30804.pdf
    Data publikacji:
    2010
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    platelet-activating factor
    supplementation
    semen
    viability
    spermatozoon
    cryopreservation
    cryopreserved spermatozoon
    dog
    adenosine triphosphate content
    sperm
    Opis:
    The aim of this study was to investigate the effect of platelet activating factor (PAF) on the quality characteristics of cryopreserved canine spermatozoa. Cryopreserved semen of 5 mixed-breed dogs was treated with different concentrations of exogenous PAF (1 × 10⁻³M, 1 × 10⁻⁴M, 1 × 10⁻⁵M and 1 × 10-6M) and examined at different time intervals (0, 30, 60 and 120 min). Cryopreserved semen treated without PAF was used as the control. Sperm quality was evaluated for motility (computer-assisted semen analysis, CASA), mitochondrial function (JC-1/PI assay) and plasma membrane integrity (SYBR-14/PI assay and Hoechst 33258). Also, ATP content of spermatozoa was determined using a bioluminescence assay. Treatment of cryopreserved semen with 1 × 10⁻³ M PAF at 120 min of incubation resulted in significantly higher total sperm motility compared with the control. It was observed that PAF-improved total sperm motility was concurrent with enhanced sperm motility patterns after treatment of cryopreserved semen. Treatment of cryopreserved semen with PAF did not improve either sperm mitochondrial function or plasma membrane integrity, as monitored by different fluorescent membrane markers. Furthermore, ATP content of cryopreserved spermatozoa was significantly higher when PAF was used at a concentration of 1 × 10⁻³ M compared with the control and other PAF treatments, regardless of the incubation time. The findings of this study indicated that treatment with 1 × 10⁻³ M PAF at 120 min of incubation rendered better quality of cryopreserved canine semen, which was associated with improved sperm motility parameters and ATP content. It can be suggested that exogenous PAF addition is beneficial as a supplement for canine semen extender used for cryopreservation.
    Źródło:
    Polish Journal of Veterinary Sciences; 2010, 13, 4
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    In vitro culture as a powerful method for conserving Iranian ornamental geophytes
    Autorzy:
    Hesami, M.
    Naderi, R.
    Yoosefzadeh-Najafabadi, M.
    Maleki, M.
    Powiązania:
    https://bibliotekanauki.pl/articles/80634.pdf
    Data publikacji:
    2018
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    in vitro culture
    plant tissue culture
    ornamental plant
    geophyte
    cryopreservation
    genetic resource
    biodiversity
    germplasm
    Iran
    Źródło:
    BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2018, 99, 1
    0860-7796
    Pojawia się w:
    BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Cryopreservation of cherry rootstock Gisela 5 (Prunus cerasus × Prunus canescens) shoot tips by droplet-vitrification technique
    Autorzy:
    Ruzic, D.
    Vujovic, T.
    Cerovic, R.
    Powiązania:
    https://bibliotekanauki.pl/articles/2189.pdf
    Data publikacji:
    2013
    Wydawca:
    Instytut Ogrodnictwa
    Tematy:
    cherry
    Gisela 5 cultivar
    rootstock
    cryopreservation
    Prunus cerasus × Prunus canescens
    droplet-vitrification technique
    shoot tip
    Źródło:
    Journal of Horticultural Research; 2013, 21, 2
    2300-5009
    Pojawia się w:
    Journal of Horticultural Research
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Encapsulation-dehydration technique for chrysanthemum “Lady Orange” cryopreservation
    Autorzy:
    Kulus, D.
    Rewers, M.
    Mikula, A.
    Powiązania:
    https://bibliotekanauki.pl/articles/951265.pdf
    Data publikacji:
    2015
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    ornamental plant
    chrysanthemum
    plant material
    horticultural production
    somaclonal variation
    cryopreservation
    long-term storage
    encapsulation-dehydration method
    Źródło:
    BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2015, 96, 1
    0860-7796
    Pojawia się w:
    BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    The effect of abscisic acid and dimethyl sulfoxide and different temperatures on the cryopreservation process of Abies nordmanniana (Steven) Spach embryogenic callus
    Autorzy:
    Nawrot-Chorabik, K.
    Sitko, K.
    Powiązania:
    https://bibliotekanauki.pl/articles/79904.pdf
    Data publikacji:
    2013
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    conference
    cryopreservation
    liquid nitrogen
    somatic embryo
    embryogenic callus
    Abies nordmanniana
    abscisic acid
    dimethyl sulphoxide
    dehydration
    callus cell
    Źródło:
    BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2013, 94, 3
    0860-7796
    Pojawia się w:
    BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Conifer somatic embryogenesis: I. Development
    Autorzy:
    Klimaszewska, K
    Cyr, D.R.
    Powiązania:
    https://bibliotekanauki.pl/articles/41304.pdf
    Data publikacji:
    2002
    Wydawca:
    Polska Akademia Nauk. Instytut Dendrologii PAN
    Tematy:
    somatic seedling
    initiation
    development
    somatic embryogenesis
    Pinaceae
    maturation
    embryogenesis
    conifer species
    forest industry
    forest
    somatic tree
    cryopreservation
    Opis:
    The discovery of conifer somatic embryogenesis (SE) and the subsequent development of SE protocols for a range of genera and species have opened new research opportunities to forest biotechnologists and a means towards mass clonal propagation for the forest industry. This paper provides a general description of the conifer SE process, followed by a review of protocols developed specifically for several conifer species in the Pinaceae family for which production of somatic trees has been demonstrated. Additionally, future research needs, including approaches for developing markers to optimize SE process and the production of high quality embryos, are discussed.
    Źródło:
    Dendrobiology; 2002, 48
    1641-1307
    Pojawia się w:
    Dendrobiology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Efficient cryopreservation by droplet vitrification of pentaploid roses and the phenotype of regenerated plants
    Autorzy:
    Pawlowska, B.
    Szewczyk-Taranek, B.
    Powiązania:
    https://bibliotekanauki.pl/articles/57879.pdf
    Data publikacji:
    2015
    Wydawca:
    Polskie Towarzystwo Botaniczne
    Tematy:
    cryopreservation
    Caninae
    droplet vitrification
    rose
    dog rose
    Rosa canina
    phenotype
    regenerated plant
    in vitro culture
    liquid nitrogen
    Opis:
    Shoot tips from in vitro plants of four rose species were cryopreserved by the droplet vitrification method. Optimized conditions involved exposure to loading solution for 20 min, then treatment with plant vitrification solution (PVS2) for 20 min (Rosa agrestis, R. canina and R. dumalis) or 30 min (R. rubiginosa) followed by freezing in liquid nitrogen. Survival rate ranged from 78.3 to 95.1%, depending on the species. Regrowth rate of shoot tips was 50.5% for R. agrestis, 63.2% for R. rubiginosa, 71.4% for R. dumalis and 78% for R. canina. The preculture of donor plants in a medium with 0.25 цЫ sucrose facilitated the isolation of shoot tips and increased regrowth rate after cryopreservation. Plant regeneration was carried out in Murashige and Skoog medium with 1 цЫ 6-benzylaminopurine, 1.5 цЫ gibberellic acid and 0.087 M sucrose. Plants regenerated from cryopreserved shoot tips did not display morphological alterations in comparison with non-cryopreserved shoot tip - derived plants.
    Źródło:
    Acta Societatis Botanicorum Poloniae; 2015, 84, 4
    0001-6977
    2083-9480
    Pojawia się w:
    Acta Societatis Botanicorum Poloniae
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Application of in vitro culture techniques in the conservation of orchids in Gdansk Pomerania
    Autorzy:
    Znaniecka, J.
    Minasiewicz, J.
    Powiązania:
    https://bibliotekanauki.pl/articles/951235.pdf
    Data publikacji:
    2015
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    conference
    in vitro culture
    orchid
    Cypripedium calceolus
    Polska
    genetic diversity
    pollination
    long-term storage
    preservation
    endangered species
    cryopreservation
    Źródło:
    BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2015, 96, 1
    0860-7796
    Pojawia się w:
    BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Kriogeniczne przechowywanie nasion
    Cryogenic storage of seeds
    Autorzy:
    Plitta, B.P.
    Michalak, M.
    Kotlarski, S.
    Chmielarz, P.
    Powiązania:
    https://bibliotekanauki.pl/articles/991637.pdf
    Data publikacji:
    2013
    Wydawca:
    Polskie Towarzystwo Leśne
    Tematy:
    nasiona
    przechowywanie
    kriokonserwacja
    ciekly azot
    nasiona z kategorii orthodox
    nasiona z kategorii recalcitrant
    liquid nitrogen
    cryopreservation
    orthodox
    suborthodox
    recalcitrant seeds
    Opis:
    Cryopreservation is a method of storage of biological material at the temperature of liquid nitrogen (–196°C, LN). The main advantage of this method is the possibility to store viable cells for a long time. Desiccation and freezing sensitivity of seeds, their fragments or other plant organs, which are useful as genetic resources, should be investigated before cryopreservation.
    Źródło:
    Sylwan; 2013, 157, 10; 723-729
    0039-7660
    Pojawia się w:
    Sylwan
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Czy kriokonserwacja jest bezpieczna? Określenie potencjalnych zmian w tkankach roślinnych poddanych kriogenicznemu przechowywaniu
    Cryopreservation - is it safe? Possible changes in cryostored plant genetic resources
    Autorzy:
    Michalak, M.
    Plitta, B.P.
    Kotlarski, S.
    Chmielarz, P.
    Powiązania:
    https://bibliotekanauki.pl/articles/990857.pdf
    Data publikacji:
    2014
    Wydawca:
    Polskie Towarzystwo Leśne
    Tematy:
    lesnictwo
    ochrona zasobow genowych
    tkanki roslinne
    kriokonserwacja
    zmiennosc fenotypowa
    zmiennosc biochemiczna
    zmiennosc genetyczna
    cryopreservation
    liquid nitrogen
    epigenetics
    preservation of genetic resources
    Opis:
    Cryopreservation (storage in liquid nitrogen, –196°C) is a technique that ensures safe, long−term conservation of plant species with recalcitrant seeds, vegetatively propagated species and biotechnology products such as somatic embryos, cell lines and genetically transformed material. The aim of cryostorage is to keep plant tissue in a safe way, which does not cause changes during storage at neither phenotypic, cytological and histological, biochemical, genetic nor epigenetic level. Therefore, before cryostorage will be applied for long−term preservation it should be thoroughly assess if it does not cause any injuries in plant material.
    Źródło:
    Sylwan; 2014, 158, 10; 795-800
    0039-7660
    Pojawia się w:
    Sylwan
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Comparison of heat transfer phenomena for two different cryopreservation methods : slow freezing and vitrification
    Autorzy:
    Skorupa, Anna
    Piasecka-Belkhayat, Alicja
    Powiązania:
    https://bibliotekanauki.pl/articles/2202057.pdf
    Data publikacji:
    2023
    Wydawca:
    Politechnika Częstochowska. Wydawnictwo Politechniki Częstochowskiej
    Tematy:
    cryopreservation
    slow freezing
    vitrification
    phase change
    finite difference method
    directed interval arithmetic
    kriokonserwacja
    powolne zamrażanie
    witryfikacja
    przemiana fazowa
    metoda różnic skończonych
    arytmetyka przedziałów skierowanych
    Opis:
    The purpose of the research is to prepare a mathematical and numerical model for the phenomenon of heat transfer during cryopreservation. In the paper, two popular methods, slow freezing and vitrification, are compared. Furthermore, the basic model of thermal processes is supplemented by the phenomenon of phase transitions. To determine the temperature distribution during cryopreservation processes, one uses the heat transfer equation proposed by Pennes. An integral part of the energy equation is the substitute thermal capacity (STC) performed according to the concept named one domain method (fixed domain method), The numerical model is developed using the finite difference method (FDM) connected with directed interval arithmetic. The final part of the article contains the results of numerical simulations.
    Źródło:
    Journal of Applied Mathematics and Computational Mechanics; 2023, 22, 1; 57--69
    2299-9965
    Pojawia się w:
    Journal of Applied Mathematics and Computational Mechanics
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Effect of various preculture and osmotic dehydration conditions on cryopreservation efficiency and morphogenetic response of chrysanthemum shoot tips
    Autorzy:
    Kulus, D.
    Serocka, M.
    Mikuła, A.
    Powiązania:
    https://bibliotekanauki.pl/articles/11880881.pdf
    Data publikacji:
    2018
    Wydawca:
    Uniwersytet Przyrodniczy w Lublinie. Wydawnictwo Uniwersytetu Przyrodniczego w Lublinie
    Tematy:
    plant cultivation
    mother chrysanthemum
    Dendranthema grandiflora
    Lady Orange cultivar
    Lady Salmon cultivar
    Richmond cultivar
    plant cultivar
    ornamental plant
    flowering plant
    chimera
    shoot tip
    explant
    cryopreservation
    dehydration
    osmotic dehydration
    morphogenetic response
    sucrose concentration
    preculture treatment
    Opis:
    The aim of this study was to evaluate the influence of sucrose concentration in the preculture medium, as well as the duration of osmotic dehydration on the efficiency of chrysanthemum ‘Richmond’, ‘Lady Orange’ and ‘Lady Salmon’ cryopreservation by encapsulation-dehydration technique. For all cultivars tested, the best regrowth of cryopreserved shot tips was recorded with 0.25 M sucrose concentration and 10 µM ABA during a two-week preculture, followed by a 4- or 7-day osmotic dehydration. The survival rate ranged from 56.8–58.0% (the Lady group) to 63.6% (‘Richmond’). However, the ability to grow was smaller and reached 18.2–50.7%. It was found that higher sucrose concentration during the preculture slowed the growth of chrysanthemum shoot tips and led to an increased formation of multiple shoots (by activating axillary buds) or deformed adventitious shoots (incapable of further growth). The frequency of tissue hyperhydricity also increased, while the rhizogenesis efficiency decreased when higher sucrose concentration in the preculture medium was applied. The influence of osmotic dehydration duration on the explants morphogenetic response was cultivar-dependent.
    Źródło:
    Acta Scientiarum Polonorum. Hortorum Cultus; 2018, 17, 1; 139-147
    1644-0692
    Pojawia się w:
    Acta Scientiarum Polonorum. Hortorum Cultus
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Frozen future – ethical questions of social egg freezing
    Zamrożona przyszłość – etyczne kwestie zamrażania jajeczek z przyczyn społecznych
    Autorzy:
    Kovács, Gusztáv
    Powiązania:
    https://bibliotekanauki.pl/articles/475269.pdf
    Data publikacji:
    2013
    Wydawca:
    Uniwersytet Opolski. Redakcja Wydawnictw Wydziału Teologicznego
    Tematy:
    social egg freezing
    oocyte cryopreservation
    ethical questions of assisted reproductive technologies
    in vitro fertilization
    family planning
    child-bearing
    responsible parenthood
    zamrażanie jajeczek z przyczyn społecznych
    zamrażanie jajeczek
    kwestie etyczne wspieranych technologii reprodukcyjnych
    zapładnianie in vitro
    planowanie rodziny
    rodzenie dzieci
    odpowiedzialne rodzicielstwo
    Opis:
    This paper aims at making a critical judgment about the practice of social egg freezing. Most papers on the ethical issues of oocyte cryopreservation for non-medical reasons seem to welcome the wide range application of the new fast freezing technology. This paper aims to challenge some of the assumptions used to justify this new practice. It does so by viewing partnership and parenthood in terms of individual biographies which need careful planning in our modern risk societies. Papers in favour of social egg freezing argue that it could serve as a tool to expand reproductive autonomy of women, and also as a means promoting gender equality. They also reason that it promoted a responsible planning of biographies, since it enabled women to postpone important decisions concerning reproductive issues or partnership. As a response I will try to show the inadequateness of these arguments by analysing the logic behind social egg freezing, and also the desires and misconceptions the method is associated with.
    Celem referatu jest krytyczny osąd stosowania zamrażania jajeczek z przyczyn społecznych. Większość prac na temat kwestii etycznych związanych z zamrażaniem jajeczek z przyczyn niemedycznych wydaje się zachęcać do szerokiego zastosowania nowej technologii szybko mrożącej. Praca ta ma na celu zakwestionować pewne założenia stosowane, by uzasadnić tę nową praktykę. Dokonuje się tego poprzez przegląd partnerstwa i rodzicielstwa w kategoriach indywidualnych biografii, które wymagają uważnego planowania w naszych współczesnych ryzykownych społeczeństwach. Prace opowiadające się za zamrożeniem jajeczek z przyczyn społecznych dowodzą, że może ono służyć jako narzędzie do rozszerzenia autonomii reprodukcyjnej kobiet, a także jako środek promujący równość genderową. Wnioskują też, że sprzyja odpowiedzialnemu planowaniu życia, gdyż umożliwia kobietom odłożenie decyzji dotyczących kwestii reprodukcji czy partnerstwa. W odpowiedzi Autor stara się pokazać nieadekwatność tych argumentów poprzez przeanalizowanie logiki ukrytej za zamrażaniem jajeczek z przyczyn społecznych, a także pragnienia i nieporozumienia, z jakimi metoda ta jest związana.
    Źródło:
    Family Forum; 2013, 3; 201-210
    2084-1698
    Pojawia się w:
    Family Forum
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-29 z 29

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