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Tytuł:
Effect of post-thaw supplementation of fractionated seminal plasma on survival of boar spermatozoa
Autorzy:
Wasilewska-Sakowska, K.
Zasiadczyk, Ł.
Fraser, L.
Strzeżek, J.
Karpiesiuk, K.
Powiązania:
https://bibliotekanauki.pl/articles/2087478.pdf
Data publikacji:
2019
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
boar
semen
fractionated seminal plasma
cryopreservation
Źródło:
Polish Journal of Veterinary Sciences; 2019, 3; 617-625
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The cryoprotective potential of propolis supplemented in frozen-thawed bull semen; biochemical and physiological findings
Autorzy:
Yeni, D.
Gülhan, M.F.
İnanç, M.E.
Avdatek, F.
Güngör, Ş.
Türkmen, R.
Tuncer, P.B.
Taşdemir, U.
Powiązania:
https://bibliotekanauki.pl/articles/16539069.pdf
Data publikacji:
2022
Wydawca:
Polska Akademia Nauk. Czasopisma i Monografie PAN
Tematy:
cryopreservation
DNA damage
oxidative stress
propolis
Opis:
In this study, the cryoprotective effect of different doses of propolis (P) on bull semen, which has solid pharmacological properties thanks to its rich phenolic components, was investigated biochemically and physiologically. Semen samples were collected from Simmental breed bulls via the artificial vagina and pooled. After dividing into five groups, control (C: no additive) and four different P (200, 100, 50, and 25 μg/mL) groups, the final concentration was diluted to 16×106 per straw. Semen samples were equilibrated at 4°C for approximately 4 hours, then placed in French straws and frozen. After thawing, sperm motility and kinetic parameters, DNA integrity by single-cell gel electrophoresis, sperm abnormalities by liquid fixation, and lipid peroxidation levels by the colorimetric method was analyzed by Computer-Assisted Semen Analyzer. P added to the diluent showed no effect on motility and kinetic parameters at P25 and P50 (p>0.05), while P100 and P200 had a negative effect (p<0.001). The addition of P (25 and 50) showed a treatment effect on tail abnormality compared to C (p<0.05). Especially P50 had a positive effect on tail length, tail DNA, and tail movement, while P100 and P200 caused DNA damage (p<0.001). MDA levels increased in all P dose groups compared to C (p<0.001). This study has clearly demonstrated that P25 and P50 supplements could be used therapeutically to treat sperm tail abnormalities and prevent DNA damage in post-thawed bull sperm.
Źródło:
Polish Journal of Veterinary Sciences; 2022, 25, 1; 5-12
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The effect of luteolin on spermatological parameters, apoptosis, oxidative stress rate in freezing rabbit semen
Autorzy:
Akarsu, S.A.
Acısu, T.C.
Güngör, İ.H.
Çakır Cihangiroğlu, A.
Koca, R.H.
Türk, G.
Sönmez, M.
Gür, S.
Fırat, F.
Esmer Duruel, H.E.
Powiązania:
https://bibliotekanauki.pl/articles/16647483.pdf
Data publikacji:
2023
Wydawca:
Polska Akademia Nauk. Czasopisma i Monografie PAN
Tematy:
rabbit
semen
cryopreservation
luteolin
oxidative stress
apoptosis
Opis:
The aim of the present study was to determine the effects of Luteolin (LUT) on semen quality, oxidative stress, apoptosis, acrosomal integrity, mitochondrial membrane potential and dead sperm ratio in rabbits. Ejaculates from six New Zealand rabbits were collected, evaluated and pooled. The pooling was divided into five groups as control (no additive) LUT 25 μM, LUT 50 μM, LUT 100 μM and LUT 200 μM and LUT added. It was then filled into a falcon tube with Tris-based extender at a final concentration of approximately 35 x 106 spermatozoa. Diluated rabbit semen samples were drawn into frozen and thawed. Frozen semen straws were thawed at 37°C in 30 seconds. According to our findings, no statistical difference was found between all doses of luteolin and the control group in the CASA (computer assisted sperm analysis) analysis performed at 4°C. However, total motility, progressive motility and rapid sperm percentage were found to be higher in the frozen and thawed rabbit semen at a dose of LUT 50 μM compared to the other groups (p<0.05). While amplitude of lateral head displacement (ALH) and beat cross-frequency (BCF) values were found at the lowest dose of LUT 200 μM, a statistically significant difference was observed between the other groups. When the flow cytometry results were examined, no statistical difference was found between the rate of dead sperm, acrosomal integrity, mitochondrial membrane potential and apoptosis rate. Morever, the H2O2 percentage was found to be lower in all experimental groups compared to the control group (p<0.001). In conclusion, the addition of LUT in long-term storage of rabbit semen provided a protective effect for spermatozoa with its antioxidative properties against damage caused by cryopreservation.
Źródło:
Polish Journal of Veterinary Sciences; 2023, 26, 1; 91-98
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Freezability and fertility of frozen-thawed boar semen supplemented with ostrich egg yolk lipoproteins
Autorzy:
Fraser, L.
Zasiadczyk, Ł.
Strzeżek, J.
Strzeżek, R.
Karpiesiuk, K.
Powiązania:
https://bibliotekanauki.pl/articles/2087726.pdf
Data publikacji:
2018
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
boar
egg yolk lipoproteins
cryopreservation
artificial insemination
Źródło:
Polish Journal of Veterinary Sciences; 2018, 21, 2; 255-263
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Interactions of ostrich egg yolk lipoproteins with seminal plasma of fractionated ejaculates and their effects on post-thaw boar semen quality
Autorzy:
Fraser, L.
Zasiadczyk, Ł.
Strzeżek, J.
Kordan, W.
Mańkowska, A.
Powiązania:
https://bibliotekanauki.pl/articles/2087484.pdf
Data publikacji:
2019
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
boar semen
ejaculate fractions
egg yolk lipoproteins
cryopreservation
Źródło:
Polish Journal of Veterinary Sciences; 2019, 3; 463-473
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Quality parameters and fertility of ram semen cryopreserved in egg yolk and soybean lecithin supplemented extenders
Autorzy:
Gogol, P.
Bryła, M.
Trzcińska, M.
Bochenek, M.
Powiązania:
https://bibliotekanauki.pl/articles/2087594.pdf
Data publikacji:
2019
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
ram semen
cryopreservation
soybean lecithin
sperm quality
fertility
Źródło:
Polish Journal of Veterinary Sciences; 2019, 1; 177-179
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Effects of spermidine on germination of Salix spp. after storage under controlled conditions
Autorzy:
Wawrzyniak, M.K.
Ley-Lopez, J.M.
Kijowska-Oberc, J.
Chmielarz, P.
Ratajczak, E.
Powiązania:
https://bibliotekanauki.pl/articles/2130180.pdf
Data publikacji:
2022
Wydawca:
Polska Akademia Nauk. Instytut Dendrologii PAN
Tematy:
hydrogen peroxide
cryopreservation
spermidine
Salix
antioxidant
seed storage
Opis:
Willows produce fast germinating and short-lived seeds, difficult to store in the long-term under controlled conditions. The aim of this study was to examine the feasibility of storage of three Salix spp. at controlled temperatures (3°, −10°, −196 °C). We also analyzed the effect of spermidine (Spd) as an antioxidant factor in desiccated seeds. Collected seeds were either desiccated or hydrated to obtain 10 levels of moisture content (between app. 4% and 2%) and subjected to storage at temperatures 3°, −10°, or −196 °C (liquid nitrogen; LN). After two months, seeds were germinated on the light at 20 °C. Seeds desiccated below a safe range of moisture content were further tested and germinated on filter paper with additions of 0.25 mM Spd solution. After 7 days seedlings were examined for hydrogen peroxide content (H2O2) and total antioxidant capacity (TAC). Fresh seeds of three Salix species: Persian willow (S. aegyptiaca L.), heartleaf willow (S. cordata Michx.) and crack willow (S. ×fragilis L.) were successfully stored at temperature −10° and −196 °C for two months. After cryopreservation seed of S. aegyptiaca, S. cordata, and S. ×fragilis germinated without viability loss in moisture content ranging from 4.4–15.9%, 6.4–18.5%, and 7.1–11.5% respectively. The addition of Spd during germination of desiccated seed did not affect germination capacity. However, seedlings of S. aegyptiaca had lower hydrogen peroxide content in comparison with control (germination on water). Seedlings of S. cordata showed an increase in hydrogen peroxide content in control after storing in LN. In seedlings of Crack willow Spd increased hydrogen peroxide content. Seeds of tested species differ in response to storage conditions. Salix seeds can be stored successfully for two months at −10° or −196 °C without losing viability in the safe range of moisture content. Storing at 3 °C can be used for storage in the narrower range of seeds’ moisture content, however, seedlings stored at this temperature produce a higher level of reactive oxygen species. Germinating seeds in Spd did not increase their germination, however in S. aegyptiaca and S. cordata decreased hydrogen peroxide content.
Źródło:
Dendrobiology; 2022, 87; 137-148
1641-1307
Pojawia się w:
Dendrobiology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Changes in cell physiology at the stage of dehydration in a cryopreservation protocol
Autorzy:
Domzalska, L.
Kedracka-Krok, S.
Mikula, A.
Rybczynski, J.J.
Powiązania:
https://bibliotekanauki.pl/articles/951293.pdf
Data publikacji:
2015
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
cryopreservation
cell physiology
dehydration
Gentiana cruciata
proteomic analysis
Źródło:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2015, 96, 1
0860-7796
Pojawia się w:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Importance of explant size and origin for cryopreservation of Rosa pomifera “Karpatia” shoot tips by a droplet vitrification method
Autorzy:
Kwasniewska, E.
Dziedzic, E.
Pawlowska, B.
Powiązania:
https://bibliotekanauki.pl/articles/951284.pdf
Data publikacji:
2015
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
cryopreservation
Rosa pomifera
shoot tip
droplet vitrification
axenic culture
Źródło:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2015, 96, 1
0860-7796
Pojawia się w:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Phytophthora Infestans: Isolation of Pure Cultures, Storage and Inoculum Preparation
Autorzy:
Sobkowiak, Sylwester
Śliwka, Jadwiga
Powiązania:
https://bibliotekanauki.pl/articles/2199698.pdf
Data publikacji:
2017-12-20
Wydawca:
Instytut Hodowli i Aklimatyzacji Roślin
Tematy:
cryopreservation
isolate
late blight
potato
rye B agar medium
Opis:
Phytophthora infestans causes potato and tomato late blight, economically the most important disease of these plant species. The Oomycete pathogen is frequently sampled, isolated to pure cultures, stored, and char-acterized. The knowledge of its diversity, migrations and evolution is essential for breeding resistant plants and for designing appropriate control strategies. The article presents methods for collection, storage and prep-aration of P. infestans isolates for inoculation of plant tissues, based on the publication by Zarzycka (2001), later updated and modified.
Źródło:
Plant Breeding and Seed Science; 2017, 76; 9-15
1429-3862
2083-599X
Pojawia się w:
Plant Breeding and Seed Science
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Bioetyczny i teologicznomoralny wymiar zamrażania ludzkich embrionów
The Bioethical and Moral Theology Dimension of Human Embryo Cryopreservation
Autorzy:
Wróbel, Józef
Powiązania:
https://bibliotekanauki.pl/articles/2035013.pdf
Data publikacji:
2020-03-30
Wydawca:
Katolicki Uniwersytet Lubelski Jana Pawła II. Towarzystwo Naukowe KUL
Tematy:
zamrażanie embrionów
ART
prokreacja wspomagana
cryopreservation of embryos
assisted procreation
Opis:
Artykuł jest poświęcony problematyce kriokonserwacji ludzkich embrionów. Stosowanie tej techniki pozostaje w ścisłym związku z prokreacją wspomaganą. Stwarzane celem zwiększenia skuteczności procedury in vitro nadliczbowe embriony zostają zamrożone, aby następnie można było je wykorzystać w kolejnych próbach transferu. Autor przedstawia techniczny wymiar zamrażania i ryzyko z nim związane. W ostatniej części Autor analizuje wymiar etyczny tych technik. Podkreśla, że chociaż sama prokreacja wspomagana jest niemoralna, to jednak moralnie słuszne jest dążenie do naprawienia tego zła przez matkę, która jest gotowa przyjąć do swojego łona stopniowo rozmrażane embriony. Dobrym rozwiązaniem wydaje się być adopcja porzuconych embrionów. Takiego rozwiązania nie da się jednak usprawiedliwić z punktu widzenia etycznego. Jeżeli rozmrożenie zostaje wymuszone przez prawo lub wymówienie umowy przez klinikę przechowywującą te embriony, możliwa jest zgoda na ich obumarcie. Z obowiązku transferu zwalnia również stan zdrowotny embrionów uniemożliwiający ich rozwój. Ponadto niemoralna jest selekcja eugeniczna embrionów oraz transfer wyłącznie tych embrionów, które mają pożądane cechy fenotypowe czy płeć.
The article discusses the issues of cryopreservation of human embryos. The use of this technique is related to assisted procreation. The supernumerary embryos created in order to increase the effectiveness of the in vitro procedure are frozen so that they can be used in subsequent transfer attempts. The author presents the technical dimension of freezing and the risks associated with it. In the last part, the author analyzes the ethical dimension of these techniques. He emphasize that although assisted procreation is immoral itself, it is morally right to strive to correct this evil by the mother, who is ready to accept into her womb the thawed embryos. The adoption of abandoned embryos seems to be a good solution. However, this solution cannot be justified from the ethical point of view. If it is forced by law to defrost or terminate the contract by the clinic that stores the embryos, it is possible to consent to their death. The health condition of the embryos, which makes their development impossible, releases from the obligation to transfer. Moreover, it is immoral to select embryos by eugenics and transfer only those embryos that have the desired phenotypic or sex characteristics.
Źródło:
Roczniki Teologiczne; 2020, 67, 3; 75-103
2353-7272
Pojawia się w:
Roczniki Teologiczne
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The quality and fertilizing ability of the ram semen cryopreserved around 40 years ago
Autorzy:
Gogol, P.
Powiązania:
https://bibliotekanauki.pl/articles/16647392.pdf
Data publikacji:
2022
Wydawca:
Polska Akademia Nauk. Czasopisma i Monografie PAN
Tematy:
ram semen
ex situ storage
gene banks
cryopreservation
sperm quality
fertility
Opis:
The aim of the study was to verify the quality of ram semen, frozen in 1982-1983, from the historical collection of the Bank of Biological Material of the National Research Institute of Animal Production. A total of 18 ejaculates from 3 Świniarka type rams were analyzed to assess sperm motility (subjectively), total motility, progressive motility, sperm concentration (CASA), membrane integrity (SYBR-14/PI) and chromatin structure (SCSA). In order to determine sperm fertilizing ability 49 ewes were intracervically inseminated (200×106 sperm per AI) with frozen- thawed semen 12 and 24 hours after detection of estrus. Sperm motility parameters, membrane intact spermatozoa and DFI did not differ among the analyzed rams. Spermatozoa concentration was significantly higher for ram no. 2 than for rams no. 1 and 3. The lambing rates (27.3 to 36.0%) did not differ significantly for individual rams. The ram semen, which had been stored for around 40 years, showed satisfactory quality and fertilizing capacity, allowing for its use in artificial insemination.
Źródło:
Polish Journal of Veterinary Sciences; 2022, 25, 4; 631-633
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
A simple way to overcome the recalcitrance of the water fern Ceratopteris thalictroides (L.) Brongn. to cryopreservation
Autorzy:
Makowski, D.
Rybczynski, J.J.
Mikula, A.
Powiązania:
https://bibliotekanauki.pl/articles/57732.pdf
Data publikacji:
2015
Wydawca:
Polskie Towarzystwo Botaniczne
Tematy:
abscisic acid
water fern
fern
Ceratopteris thalictroides
cryopreservation
encapsulation-dehydration method
darkness
Opis:
Ceratopteris thalictroides is a water fern very sensitive to both dehydration and low temperature. This study focuses on the cryopreservation of this species by encapsulation-dehydration technique, in particular on the effects of pre-culture step, alginate bead size and the physical conditions of culture on the cryopreservation efficiency. Encapsulated and non-pre-cultured gametophytes did not survive cooling with liquid nitrogen. When cryopreservation was preceded by a 2-week period of pre-culture, regrowth reached 42.1%. Reduction in the size of the alginate bead, and culture in total darkness resulted in improved gametophyte regrowth capacity (75.5% or 81.7%, respectively). The best results (91.3%) were obtained when all factors tested occurred simultaneously. The gametophytes recovered very quickly and sporophytes were formed within 4 weeks after rewarming. These simple improvements can be used, not only for the cryopreservation of gametophytes in cryptogams but also for some recalcitrant species of seed plants.
Źródło:
Acta Societatis Botanicorum Poloniae; 2015, 84, 3
0001-6977
2083-9480
Pojawia się w:
Acta Societatis Botanicorum Poloniae
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Sensitivity of Tilia cordata seeds to dehydration and temperature of liquid nitrogen
Autorzy:
Chmielarz, P
Powiązania:
https://bibliotekanauki.pl/articles/41625.pdf
Data publikacji:
2002
Wydawca:
Polska Akademia Nauk. Instytut Dendrologii PAN
Tematy:
dehydration
drying
liquid nitrogen
temperature
Tilia cordata
forest habitat
sensitivity
scarification
cryopreservation
seed
Opis:
The aim of the study was to assess the susceptibility of small-leaved lime (Tilia cordata Mill.) seeds to drying and freezing in liquid nitrogen (-196°C). Seed samples were frozen in liquid nitrogen for 24 h at 11 different levels of seed moisture content (m.c.), ranging from 3.1% to 22.8% (fresh weight basis). All samples, including unfrozen control samples, were subjected to scarification with concentrated sulphuric acid (Tylkowski 1998) either before or after freezing. Seed pre-treatment before germination (at 3~15°C/16~8h) involved cold stratification at 3°C without substrate. Seed drying to 3.1% m.c. significantly reduced their germinability (to 63%), as compared to the high germinability (82-88%) of seeds with 5.2-20.9% m.c. Thus seeds of this species can be assigned to the ‘suborthodox' category. Such a high germinability (79-87%) was preserved after freezing in liquid nitrogen in samples dried to 9.0-17.4% m.c. if scarification was performed before freezing, and in samples dried to 9.1-16.2% m.c. if scarification was performed after freezing. The highest percentage of seedlings emerged after freezing in liquid nitrogen from seeds dried to 11.1-20.1% m.c. (emergence 65-75%) if scarification was performed before freezing, and from seeds dried to 7.3-17.8% m.c. (emergence 53%-71%) if scarification was performed after freezing.
Źródło:
Dendrobiology; 2002, 47 Supplement
1641-1307
Pojawia się w:
Dendrobiology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Changes in DNA methylation of embryonic axes of seeds of oak and beech during in vitro culture and cryopreservation
Autorzy:
Nuc, K.
Marszalek, M.
Pukacki, P.M.
Powiązania:
https://bibliotekanauki.pl/articles/80269.pdf
Data publikacji:
2013
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
conference
DNA methylation
plant growing
embryonic axis
seed
oak
beech
in vitro culture
cryopreservation
Źródło:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2013, 94, 3
0860-7796
Pojawia się w:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
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