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Wyszukujesz frazę "anther culture" wg kryterium: Temat


Tytuł:
Biometrical characteristics of $R_2$ generation of anther-derived pepper (Capsicum spp.) plants
Charakterystyka biometryczna linii otrzymanych w kulturach pylników mieszańców papryki (Capsicum spp.)
Autorzy:
Kisiała, A.
Olszewska, D.
Niklas-Nowak, A.
Nowaczyk, P.
Powiązania:
https://bibliotekanauki.pl/articles/1576065.pdf
Data publikacji:
2011
Wydawca:
Polskie Towarzystwo Botaniczne
Tematy:
Capsicum
anther culture
morphological characteristics
androgenic regenerants
Opis:
Eight anther-derived DH lines of pepper hybrids: two red-fruited (AP14, AP15) and two yellow lines (AP25 and AP32) of C. annuum (ATZ1 × PO)F2, two lines of (C. frutescens × C. annuum)F1 (FA1, FA2), and two of (C. frutescens × C. chinense)F1 (FCH2, FCH3), were studied regarding important morphological plant and fruit characters. C. annuum breeding line ‘ATZ1’ was used as a standard. The following traits were evaluated: total fruit yield, fruit weight, weight of placenta with seeds, technological matter, pericarp thickness, extract content, dry matter content, weight and number of seeds per fruit. The level of homogeneity within the DH lines was analysed with a one-way analysis of variance, additionally the values of coefficient of variation (CV) were determined for the tested plant characters. The highest phenotypic uniformity was noted for the red-fruited lines obtained from anthers of (ATZ1 × PO)F2 and for the DH lines of (C. frutescens × C. annuum) F1, while the least uniform were FCH2 and FCH3 lines of (C. frutescens × C. chinense)F1. The DH lines AP14 and AP15, as well as FA1 and FA2 were phenotype homogeneous in respect of weight of fruit, technological matter, pericarp thickness and extract content. For these parameters, also the values of CV were the lowest. Statistically significant interline polymorphism between the androgenic lines of the same origin was detected among the lines of C. annuum (ATZ1 × PO)F2.
W pracy oceniono wybrane cechy plonotwórcze ośmiu linii DH, otrzymanych w kulturach pylników mieszańców papryki (Capsicum spp.): C. annuum (ATZ1 × PO) F2 (dwie linie czerwonoowocowe – AP14, AP15 i dwie żółtoowocowe – AP25 i AP32), (C. frutescens × C. annuum) F1 (FA1, FA2) oraz (C. frutescens × C. chinense) F1 (FCH2, FCH3). Linia hodowlana C. annuum ATZ1 została wykorzystana jako standard do porównań. Oceniano całkowity plon owoców z rośliny, masę owocu, masę łożyska z nasionami, masę technologiczną, grubość ścian perykarpu, zawartość ekstraktu i suchej masy oraz masę i liczbę nasion z owocu. Wyrównanie każdej z linii określono przy pomocy analizy wariancji oraz współczynnika zmienności cech (CV). Najwyższą jednorodność fenotypową roślin stwierdzono w przypadku czerwonoowocowych linii DH, otrzymanych w kulturach pylników mieszańców (ATZ1 × PO) F2 oraz (C. frutescens × C. annuum) F1. Linie AP14, AP15 oraz FA1 i FA2 były wyrównane między innymi pod względem masy całkowitej oraz technologicznej owocu, a także grubości ścian perykarpu i zawartości ekstraktu. Dla tych cech odnotowano również najniższe wartości CV. Najmniej wyrównane fenotypowo okazały się linie FCH2 i FCH3, pochodzące z kultur pylników międzygatunkowego mieszańca (C. frutescens × C. chinense) F1. Istotny statystycznie, międzyliniowy polimorfizm cechował cztery androgeniczne linie wewnątrzgatunkowego mieszańca C. annuum (ATZ1 × PO)F2.
Źródło:
Acta Agrobotanica; 2011, 64, 3; 53-58
0065-0951
2300-357X
Pojawia się w:
Acta Agrobotanica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Androgenic potential of polish barley cultivars in anther culture in vitro
Autorzy:
Kruczkowska, H.
Pawłowska, H.
Skucińska, B.
Powiązania:
https://bibliotekanauki.pl/articles/2198872.pdf
Data publikacji:
2005-06-23
Wydawca:
Instytut Hodowli i Aklimatyzacji Roślin
Tematy:
androgenesis
anther culture
barley
cold or mannitol pretreatment
Opis:
The aim of the research was to assess the androgenic potential of 21 Polish cultivars of spring and winter barley. On average, a few green plants developed per 100 anthers. Cold pretreatment proved conducive to the formation of embryos/calli, while mannitol pretreatment favoured green plant development. The first embryos/calli formed on the induction medium developed most efficiently. The authors chose FHG medium supplemented with 1 mg/l BAP and 0.2 mg/l 2,4-D (induction of androgenesis) and FHG medium supplemented with 0.4 mg/l BAP and 3% maltose (plant development).
Źródło:
Plant Breeding and Seed Science; 2005, 51; 57-66
1429-3862
2083-599X
Pojawia się w:
Plant Breeding and Seed Science
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Comparison of the efficiency of haploid and doubled haploid induction in anther cultures of winter and spring forms of triticale (× Triticosecale Wittm.)
Autorzy:
Slusarkiewicz-Jarzina, A.
Ponitka, A.
Pudelska, H.
Wozna, J.
Powiązania:
https://bibliotekanauki.pl/articles/951234.pdf
Data publikacji:
2015
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
doubled haploid
anther culture
homozygous line
triticale
Triticosecale
androgenesis
Źródło:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2015, 96, 1
0860-7796
Pojawia się w:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Factors influencing the efficiency of wheat anther culture
Autorzy:
Lantos, C.
Pauk, J.
Powiązania:
https://bibliotekanauki.pl/articles/2117772.pdf
Data publikacji:
2021
Wydawca:
Polska Akademia Nauk. Czasopisma i Monografie PAN
Tematy:
androgenesis
anther culture
doubled haploid
Triticum aestivum L.
wheat
Opis:
Using in vitro androgenesis serves as a unique opportunity to produce doubled haploid (DH) plants in many species. More benefits of this biological phenomenon have kept these methods in the focus of fundamental research and crop breeding for decades. In common wheat (Triticum aestivum L.), in vitro anther culture is one of the most frequently applied DH plant production methods. The efficiency of in vitro wheat anther culture is influenced by many factors, such as the genotype, growing conditions, collection time, pre-treatments, and compositions of media and culture conditions. According to some critical review, the genotype dependency, low efficiency and albinism are mentioned as limitations of application of the anther culture method. However, some research groups have made significant efforts to diminish the effects of these bottlenecks. Due to the improvements, a well-established in vitro anther culture method can be an efficient tool in modern wheat breeding programs.
Źródło:
Acta Biologica Cracoviensia. Series Botanica; 2020, 62, 2; 7-16
0001-5296
Pojawia się w:
Acta Biologica Cracoviensia. Series Botanica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Effect of donor plant treatment with 2,4-dichlorophenoxyacetic acid on the anther culture of hot Capsicum spp.
Autorzy:
Nowaczyk, L.
Nowaczyk, P.
Olszewska, D.
Niklas-Nowak, A.
Powiązania:
https://bibliotekanauki.pl/articles/951309.pdf
Data publikacji:
2015
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
androgenesis
Capsicum
capsaicinoid
2,4-dichlorophenoxyacetic acid
anther culture
microspore
Źródło:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2015, 96, 1
0860-7796
Pojawia się w:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Anther and microspore cultures of barley and wheat
Autorzy:
Kasha, K J
Ziauddin, A
Cho, U H
Simion, E
Petroski, R
Cistue, L
Powiązania:
https://bibliotekanauki.pl/articles/2046611.pdf
Data publikacji:
1997
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
transformation
isolated microspore
doubled haploid
wheat
microspore culture
barley
anther culture
Opis:
This paper briefly cites the various procedures for the production of doubled haploids in barley and wheat. Various associated terms are defined and the factor involved in haploid production are outlined. Isolated microspore cultures offer some advantages over anther culture. Our current procedures for isolated microspore culture of barley and wheat are presented and compared.
Źródło:
Journal of Applied Genetics; 1997, 38, 4; 373-380
1234-1983
Pojawia się w:
Journal of Applied Genetics
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The androgenic response in anther culture of spring wheat is greater in genotypes with solid stem in contrast to genotypes with hollow stem
Autorzy:
Weigt, D.
Kiel, A.
Nawracala, J.
Pluta, M.
Powiązania:
https://bibliotekanauki.pl/articles/951280.pdf
Data publikacji:
2015
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
anther culture
spring wheat
stem
genotype
water-soluble carbohydrate
androgenesis
breeding programme
Źródło:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2015, 96, 1
0860-7796
Pojawia się w:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Wheat anther culture as affected by various cultural changes and supplements
Autorzy:
Simonson, R L
Baenziger, P S
Gustafson, V D
Powiązania:
https://bibliotekanauki.pl/articles/2046627.pdf
Data publikacji:
1997
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
cultural change
supplement
wheat
silver nitrate
Triticum aestivum
anther culture
cereal tissue
Opis:
With the numerous improvements in cereal tissue and wheat anther culture, it is necessary to determine which of the improvements should be combined for optimal response. This study was conducted using one highly responsive cultivar of wheat (Triticum aestivum L. cv. Pavon 76) to test the effectiveness of pre-culture cold treatment (0 or 3-7 days at 5°C) of anthers, five initiation basal media, and various changes in Murashige-Skoog regeneration media. A cold pre-culture treatment was inhibitory for all initiation media for embryoid initiation. Of the initiation media, P1, 85D12, and N6 were similar for embryoid initiation (0.80 to 0.90 embryoids/anther) without a cold pre-culture treatment. Plant regeneration was improved by the addition of amino acids or glucose, increased sucrose concentration, filter sterilizing the medium, and altering plant growth regulator concentrations. P1 medium which is normally used for embryoid initiation was also beneficial for improving plant regeneration. Ethylene inhibitors were generally not beneficial.
Źródło:
Journal of Applied Genetics; 1997, 38, 4; 381-392
1234-1983
Pojawia się w:
Journal of Applied Genetics
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Effect of genotype and media composition on embryoid induction and plant regeneration from anther culture in triticale
Autorzy:
Slusarkiewicz-Jarzina, A
Ponitka, A
Powiązania:
https://bibliotekanauki.pl/articles/2046679.pdf
Data publikacji:
1997
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
plant regeneration
microspore
triticale
haploid plant
anther culture
embryonic structure
androgenesis
somatic embryo
Opis:
Anthers of twenty triticale genotypes were cultured on three different media: 1 - PII (Chuang et al. 1978) with increased 2,4-D to 2 mg L⁻¹ and agarose 6 g L⁻¹ , 2 - Macro-, micronutrients and vitamines like in MN6 (Chu, Hill 1988) + 2 mg L⁻¹ 2.4-D + 0.5 mg L⁻¹ KIN + 5 mg L⁻¹ FeEDTA + 90 g L⁻¹ sucrose; 3 - Macro-, micronutrients and vitamines like in MN6 (Chu, Hill, 1988) + 2 mg L⁻¹ 2.4-D + 0.5 mg L⁻¹ KIN + 5 mg L⁻¹ FeEDTA + 120 g L⁻¹ sucrose. Embryoid induction and plant regeneration were influenced by donor plant genotype and induction medium. Medium 1 was the best for embryoid induction, while for green plant regeneration the best were media 1 and 2. Out of 300 anthers from each genotype plated on each of the three media, 64-1250, 12-486 and 6-212 somatic embryos and 8-86, 3-136 and 1-26 green plants were recorded, on media 1, 2 and 3, respectively.
Źródło:
Journal of Applied Genetics; 1997, 38, 3; 253-258
1234-1983
Pojawia się w:
Journal of Applied Genetics
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Otrzymywanie spontanicznych i indukowanych linii podwojonych haploidów pszenżyta ozimego z wykorzystaniem kultur pylnikowych
Production of spontaneous and induced doubled-haploid lines of winter triticale obtained through anther culture
Autorzy:
Ponitka, Aleksandra
Ślusarkiewicz-Jarzina, Aurelia
Powiązania:
https://bibliotekanauki.pl/articles/2198115.pdf
Data publikacji:
2011-09-30
Wydawca:
Instytut Hodowli i Aklimatyzacji Roślin
Tematy:
haploid
kultury pylnikowe
linie DH
pszenżyto ozime
anther culture
DH lines
winter triticale
Opis:
W kulturach pylnikowych 15 form mieszańcowych pszenżyta ozimego badano częstotliwość uzyskiwania androgenicznych roślin. Ze wszystkich genotypów otrzymano zielone rośliny, przy czym częstotliwość wahała się od 0,4 do 15,2/100 pylników w poszczególnych genotypach. Dla regeneracji roślin zastosowano pożywkę 190-2 z dwoma stężeniami kinetyny (0,5 lub 1,5 mg/l). Na pożywce zawierającej 0,5 mg/l kinetyny otrzymano więcej zielonych roślin, tj. od 2,5 do 46,7/100 androgenicznych struktur. Poziom ploidalności oznaczono cytometrycznie u 350 roślin i w zależności od genotypu stwierdzono od 17,6 do 80,0% spontanicznie podwojonych haploidów. Ogółem uzyskano 80,3% linii DH pszenżyta ozimego, wśród których było 38,0% spontanicznie podwojonych haploidów, natomiast w wyniku kolchicynowania 209 roślin haploidalnych otrzymano 70,8% linii DH.
We investigated the rate of obtaining androgenic plants using anther culture of 15 winter triticale hybrids. Green plants were obtained from all genotypes, with the frequency ranging from 0.4 to 15.2/100 anthers. The medium 190-2, with two different concentrations of kinetin (0.5 and 1.5 mg/l), was used for plant regeneration. Higher number of green plants was observed in the case of the medium with 0.5 mg/l of kinetin (2.5–46.7/ 100 androgenic structures). Ploidy level was determined by flow cytometry in 350 plants and showed from 17.6 to 80.0% spontaneously doubled haploids, depending on the genotype. A total of 80.3% of DH lines was obtained, among which 38.0% were spontaneously doubled haploids, whereas 70.8% of DH lines was obtained from colchicine treatment of 209 haploid plants.
Źródło:
Biuletyn Instytutu Hodowli i Aklimatyzacji Roślin; 2011, 260/261; 183-191
0373-7837
2657-8913
Pojawia się w:
Biuletyn Instytutu Hodowli i Aklimatyzacji Roślin
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Induced androgenesis in Phleum pratense
Indukowana androgeneza w Phleum pratense
Autorzy:
Broda, Z.
Mikołajczyk, S.
Powiązania:
https://bibliotekanauki.pl/articles/2234853.pdf
Data publikacji:
2006
Wydawca:
Polskie Towarzystwo Łąkarskie
Tematy:
induced androgenesis
androgenesis
Phleum pratense
anther culture
genotype
timothy
medium
anther treatment
homozygous line
in vitro culture
haploid
microspore
plant production
Źródło:
Łąkarstwo w Polsce; 2006, 09; 33-40
1506-5162
Pojawia się w:
Łąkarstwo w Polsce
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Gene segregation in a barley DH population
Autorzy:
Szarejko, I
Maluszynski, M
Polok, K
Zbieszczyk, J
Powiązania:
https://bibliotekanauki.pl/articles/2046613.pdf
Data publikacji:
1997
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
doubled haploid
barley
haploid plant
gametoclonal variation
gametic selection
anther culture
population
gene segregation
Opis:
A large population of anther culture-derived barley regenerants and their progeny was tested for allele segregation at 1 isozyme and 8 morphological marker loci. The segregation of genetic markers was examined separately for haploid, diploid and polyploid regenerants. All the 9 analysed genes except al (albino lemma) on chromosome 3 segregated according to the expected 1:1 ratio in the microspore-derived barley population. There was no difference in allele distribution between haploid and diploid regenerants. Among the limited number of 34 analysed tetraploids a significant excess of the dominant allele at locus о (orange lemma) of chromosome 6 was also observed. The recombination frequency between linked genes (n - lk2 on chromosome 1 and r - s on chromosome 7) estimated in the DH population did not differ significantly from recombination rates calculated in F₂ progeny or presented in barley chromosome maps. The phenomenon of gametic selection is discussed in relation to the genotype dependency of anther culture response and procedures used for DH production in barley.
Źródło:
Journal of Applied Genetics; 1997, 38, 4; 415-424
1234-1983
Pojawia się w:
Journal of Applied Genetics
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Evaluation of the regeneration ability of Brassica interspecific hybrids in in vitro anther culture
Autorzy:
Wojciechowski, A.
Niemann, J.
Powiązania:
https://bibliotekanauki.pl/articles/951274.pdf
Data publikacji:
2015
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
anther culture
haploid plant
homozygous line
interspecific hybrid
Brassica
regeneration ability
in vitro condition
Źródło:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2015, 96, 1
0860-7796
Pojawia się w:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Influence of genetic and environmental factors on anther culture response of wheat
Autorzy:
Zhou, H
Konzak, C F
Powiązania:
https://bibliotekanauki.pl/articles/2046623.pdf
Data publikacji:
1997
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
callus response
plant regeneration
environmental factor
doubled haploid
wheat
genetic factor
Triticum aestivum
anther culture
Opis:
The influences of genetic and environmental factors on the anther culture responses of wheat were investigated. Significant differences for callus induction, plant regeneration, and green plant percentages were observed when the nucleus of Triticum acstivum L. cv. Selkirk was transferred to ten alien cytoplasms by substitution backcrosses. In most cases, the alien cytoplasms decreased anther culture responses, but sometimes they were as good as or better than the T. aestivum cytoplasm. Significant within-genotype variation for anther culture responses were observed for wheat varieties Chris, Yecora Rojo, WA7176 and Edwall, indicating genetic heterogeneity in the present commercial cultivars, and potential for improving anther culture responses by in vitro prescreening. When five genotypes (Chris, Pavon 76, Butte 86, WA6916, and Edwall) were cultured across three (potato-4 liquid, 100 g L⁻¹ ficoll-supplemented, and 6 g L⁻¹ agar-solidified) induction media, the liquid and ficoll-containing media were 10 to 15 times more productive than the agar-solidified medium. Whereas, the ficoll medium was not significantly different from the liquid medium. Several low concentration starch media appeared promising to replace current induction media. The starch media sustained the highcallus-induction properties of the liquid medium, while improving callus aeration similar to that observed on solid media, resulting in markedly higher plant regeneration and green plant percentages.
Źródło:
Journal of Applied Genetics; 1997, 38, 4; 393-406
1234-1983
Pojawia się w:
Journal of Applied Genetics
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Regeneration of oat androgenic plants in relation to induction media and culture conditions of embryo-like structures
Autorzy:
Ponitka, A
Slusarkiewicz-Jarzina, A.
Powiązania:
https://bibliotekanauki.pl/articles/57988.pdf
Data publikacji:
2009
Wydawca:
Polskie Towarzystwo Botaniczne
Tematy:
oat
Avena sativa
hybrid
plant regeneration
haploid plant
doubled haploid
anther culture
embryo-like structure
culture condition
Opis:
The effect of C17 and W14 induction media on the formation of embryo-like structures (ELS) from F3 generation of nine hexaploid oat hybrids was investigated in the study. In all genotypes, the highest number of ELS (0.6 - 12.1/100 anthers) was obtained on C17 medium. The efficiency of plant regeneration on medium 190-2 was tested, in relation to different ELS culture conditions. The highest rate of green plants per 100 ELS (3.3 - 42.4) was produced by incubation at 22oC in the dark for the first two weeks. Among 36 green regenerants, 28 (77.8%) were haploid and 8 (22.2%) were spontaneous doubled haploids, fully fertile. After colchicine treatment of haploid plants, 19 were partially fertile and set from 1 to 15 seed per panicle.
Źródło:
Acta Societatis Botanicorum Poloniae; 2009, 78, 3; 209-213
0001-6977
2083-9480
Pojawia się w:
Acta Societatis Botanicorum Poloniae
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
A comparison of anther culture and maize pollination for haploid production in wheat
Autorzy:
Fedak, G
Burvill, M
Voldeng, H D
Powiązania:
https://bibliotekanauki.pl/articles/2046608.pdf
Data publikacji:
1997
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
plant production
haploid production
F1 hybrid
green plant
growth regulator
wheat
pollination
haploid
anther culture
maize
Opis:
The use of 2,4-D as a post pollination treatment to fertilize florets instead of GA₃ provided a two-fold improvement in seed set, culturable embryos and hence green plant production from wheat and maize pollination. The efficiency of the wheat by maize pollination method for haploid production was equal to the anther culture method in F₁ hybrid combinations that were responsive to anther culture. However, in recalcitrant combinations obtained from non-responsive genotypes the maize pollination method was far superior.
Źródło:
Journal of Applied Genetics; 1997, 38, 4; 407-414
1234-1983
Pojawia się w:
Journal of Applied Genetics
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Otrzymywanie podwojonych haploidów z mieszańców ozimych i jarych pszenżyta z zastosowaniem kolchicyny w kulturach pylnikowych
Doubled haploid production of winter and spring triticale hybrids using colchicine in anther cultures
Autorzy:
Ślusarkiewicz-Jarzina, Aurelia
Ponitka, Aleksandra
Powiązania:
https://bibliotekanauki.pl/articles/2198759.pdf
Data publikacji:
2015-06-30
Wydawca:
Instytut Hodowli i Aklimatyzacji Roślin
Tematy:
kolchicyna
kultury pylnikowe
podwojone haploidy
pszenżyto ozime
pszenżyto jare
colchicine
anther culture
doubled haploids
winter triticale
spring triticale
Opis:
Celem pracy było podwyższenie efektywności otrzymywania podwojonych haploidów w kultu¬rach pylnikowych 6 form mieszańcowych pszenżyta ozimego i 6 form jarych poprzez zastosowanie kolchicyny w pożywce indukującej androgenezę. Modyfikowano stężenie kolchicyny i czas traktowania (0,1 mg/l lub 1,0 mg/l kolchicyny odpowiednio przez 24, 48 i 72 godziny) w płynnej pożywce C17. Stwierdzono, że optymalnymi parametrami zastosowania kolchicyny były stężenia 1,0 mg/l przez 24 godziny oraz 0,1 mg/l przez 48 godzin. Na podstawie analiz cytometrycznych, w tych warunkach stwierdzono zwiększenie liczby roślin o podwojonej liczbie chromosomów. Wynik ten obserwowano u 6 form ozimych (średnio 66,2%) oraz u 2 form jarych (średnio 52,7%), w porów¬naniu z warunkami kontrolnymi na pożywce bez kolchicyny, w których uzyskano około 30% podwojonych haploidów.
The aim of this work was to increase the efficiency of doubled haploids production in anther cultures of 6 winter and 6 spring triticale hybrids. The colchicine concentration and the time of its use (0.1 mg/l or 1.0 mg/l colchicine, for 24, 48 and 72 h, respectively) in androgenesis inducing C17 liquid medium were modified. It was found that the optimal parameters of colchicine treatment were 1.0 mg/l for 24 hours, and 0.1 mg/l for 48 hours. Based on flow cytometry analysis, in those conditions an increase in a number of doubled haploids was observed. The increase was observed in 6 forms of winter (average 66.2%) and 2 forms of spring triticale (average of 52.7%), as compared to control conditions (the C17 medium without colchicine), producing of about 30.0% of doubled haploids
Źródło:
Biuletyn Instytutu Hodowli i Aklimatyzacji Roślin; 2015, 276; 57-67
0373-7837
2657-8913
Pojawia się w:
Biuletyn Instytutu Hodowli i Aklimatyzacji Roślin
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Application of in vitro culture techniques to barley [Hordeum vulgare L.] improvement
Autorzy:
Ullrich, S E
Kleinhofs, A.
Hou, L.
Jones, B.L.
Powiązania:
https://bibliotekanauki.pl/articles/2044453.pdf
Data publikacji:
1998
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
tissue culture
doubled haploid
mutagenesis
breeding programme
anther culture
in vitro
callus culture
somaclonal variation
barley
mutation
Hordeum vulgare
embryo
molecular biology
Opis:
Several aspects of in vitro culture have potential for cereal improvement. This paper focuses on evaluation of somaclonal variation (SV) from immature embryo callus culture, and doubled haploid (DH) production via anther culture in barley. Genetically stable SV was observed for several seedling morphological traits such as albino, yellow, light green and lethal. SV occurred at approximately half the frequency of azide-induced mutagenesis. The potential for widespread application of anther culture-mediated DH production in barley breeding and genetic studies was increased through culture procedure improvements and understanding the inheritance of anther culture response. Methodology improvements included substitution of inexpensive gelrite for expensive ficoll or agarose, ability to grow anther donor plants under field as well as growth chamber conditions and flexibility in cold pretreatment/storage of anther donor spikes for 4-6 weeks prior to anther plating. From diallel analysis, inheritance of anther culture response was complex with additive and dominance effects for embryoid formation, total plant regeneration and green plant regeneration and reciprocal effects (maternal) for green plant regeneration. High x low responder crosses generated F₁’s that were intermediate in response and low x low crosses sometimes produced F₁ heterosis for green plant regeneration. Therefore, some recalcitrant types appear to be usable in anther culture DH production systems within a breeding program.
Źródło:
Journal of Applied Genetics; 1998, 39, 1; 49-58
1234-1983
Pojawia się w:
Journal of Applied Genetics
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Cytological and genetic evaluation of anther culture-derived doubled haploids in barley
Autorzy:
Szarejko, I
Falk, D E
Janusz, A
Nabialkowska, D
Powiązania:
https://bibliotekanauki.pl/articles/2046612.pdf
Data publikacji:
1997
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
cytological analysis
doubled haploid
morphological variation
genotype
barley
genetic evaluation
gametoclonal variation
chlorophyll mutation test
Hordeum vulgare
anther culture
Opis:
Gametoclonal variation among anther culture-derived plants of three barley genotypes was estimated on the basis of cytological analysis (DH1, DH2 generation), observation of morphological variants (DH2, DH3) and chlorophyll mutation test (DH2, DH3). Individual head rows were grown in the field to detect possible chimeric structure of regenerants and to assess the number of variants and mutations in each line. Spontaneously doubled plants were the most frequent class (70%) among regenerants and almost 90% of them were completely fertile. There was a difference in proportion of haploids produced by different genotypes, but the highest frequency observed did not exceed 21%. The remaining regenerants were tetraploid, and contained chromosomal mutations or chimeras. In total, there were about 15% of polyploids and plants carrying chromosomal aberrations (translocations, inversions) among DH1 individuals. The changes in chromosome number and structure were the main source of observed variation. The level of gene mutation induced in vitro was relatively low. No more than 1% of microspore-derived plants expressed visible morphological changes in DH2 progeny. Only two morphological variants derived from the Bruce cultivar proved to be homozygous mutants (dwarf type) stable up the to third generation. The frequency of DH plants carrying chlorophyll mutation was 5.8%, but most of them (82%) were chimeric and had only a small mutation sector. The level of gametoclonal variation depended on the donor plant genotype. The highest proportion of variants and mutations was observed among DH plants derived from the Bruce cultivar, while the lowest was recorded among plants regenerated from anther culture of the doubled haploid line H930-36. Mechanisms leading to the observed variation and implications resulting from the presented experiments concerning implementation of anther culture in barley breeding were discussed. It was concluded that this method resulted in a high frequency of spontaneous doubling, a low frequency of genetic changes, and being less time and effort-consuming than the ’Bulbosum’ technique, can be applied to most barley breeding programs.
Źródło:
Journal of Applied Genetics; 1997, 38, 4; 437-452
1234-1983
Pojawia się w:
Journal of Applied Genetics
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Rice improvement, involving altered flower structure more suitable to cross-pollination, using in vitro culture in combination with mutagenesis
Autorzy:
Min, S K
Powiązania:
https://bibliotekanauki.pl/articles/2048292.pdf
Data publikacji:
1998
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
japonica rice
radiation
China
mutagenesis
mutant
anther culture
in vitro
cross-pollination
hybridization
indica rice
somatic cell
Oryza sativa
rice
Opis:
Anther and somatic tissue culture in combination with mutagenesis were carried out to evaluate the efficiency of different mutagenic treatments of various in vitro culture materials, and to obtain some promising variants for rice improvement. Results indicated that in japonica rice radiation treatment of dry seeds and young panicles influenced the percentage of green plantlets regeneration from anther culture. Both treatments increased significantly the percentage of regenerated green plantlets in comparison with the control. Irradiation with 30 Gy of rice callus increased also the percentage of regenerated green plantlets. For indica rice, the combination of the suitable dose of gamma rays irradiation on seeds and an improved medium, increased the percentage of callus induction. This approach made it possible to use anther culture in indica rice breeding. Somatic tissue cultures combined with radiation-induced mutagenesis led to the development of a number of promising mutants including some new cytoplasm-nucleus interacting male-sterile lines with almost 100% stigma exsertion. Their development would be of practical significance for increasing the genetic diversity for production of hybrid rice.
Źródło:
Journal of Applied Genetics; 1998, 39, 2; 151-162
1234-1983
Pojawia się w:
Journal of Applied Genetics
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Ocena zdolnosci pylnikow wybranych odmian lnu oleistego [Linum usitatissimum L.] do tworzenia kalusa
Estimation of the ability of anthers from different linseed cultivars [Linum usitatissimum L.] for callus induction
Autorzy:
Wielgus, K
Mankowska, G.
Powiązania:
https://bibliotekanauki.pl/articles/832665.pdf
Data publikacji:
2006
Wydawca:
Instytut Hodowli i Aklimatyzacji Roślin
Tematy:
rosliny oleiste
len oleisty
Linum usitatissimum
odmiany roslin
pozywki hodowlane
kultury in vitro
kultury pylnikowe
kalus
oil plant
linseed
plant cultivar
culture medium
in vitro culture
anther culture
callus
Źródło:
Rośliny Oleiste - Oilseed Crops; 2006, 27, 2; 213-222
1233-8273
Pojawia się w:
Rośliny Oleiste - Oilseed Crops
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Anther culture response and genetic relationships between Iranian and European barley (Hordeum vulgare L.) cultivars
Autorzy:
Izadi, S.
Cheghamirza, K.
Kahrizi, D.
Powiązania:
https://bibliotekanauki.pl/articles/80731.pdf
Data publikacji:
2017
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
barley
Hordeum vulgare
cereal grain
anther culture response
callus induction
genetic diversity
inter-simple sequence repeat marker
Mantel test
Iranian barley
European barley
Źródło:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2017, 98, 4
0860-7796
Pojawia się w:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Production and evaluation of doubled haploid wheat lines
Autorzy:
Pauk, J
Kertesz, Z
Powiązania:
https://bibliotekanauki.pl/articles/2046625.pdf
Data publikacji:
1997
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
wheat line
production
doubled haploid
cross combination
haploid plant
anther response
Triticum aestivum
anther culture
F3 wheat line
F1 population
F4 wheat line
F2 population
combining ability
Opis:
F₁, F₂ populations as well as F₃ and F₄ wheat lines coming from more than 300 cross combinations have been used for androgenic wheat studies and more than 5000 haploid plants were produced. Promising doubled haploid lines were tested together with lines produced in the conventional way in yield trials. The two best doubled haploid lines overyielded the standard. On the basis of results related to the general combining ability it is advisible to cross parents with a good combining ability for characters such as high percentage of responding anthers and embryo/callus induction with parents indicating a good combining ability for green plant production. In this way ideal lines can be obtained for doubled haploid production.
Źródło:
Journal of Applied Genetics; 1997, 38, 4; 425-435
1234-1983
Pojawia się w:
Journal of Applied Genetics
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Induction of multi-nucleate microspores in anther culture of salix viminalis L.
Autorzy:
Wojciechowicz, M K
Kikowska, M.A.
Powiązania:
https://bibliotekanauki.pl/articles/41619.pdf
Data publikacji:
2009
Wydawca:
Polska Akademia Nauk. Instytut Dendrologii PAN
Tematy:
Salix viminalis
genotype
androgenesis
thermal factor
medium composition
stress response
thermal shock
anther culture
multinucleate microspore
male clone
mannitol
Kyo medium
microspore
ultrastructural analysis
initial microspore
Opis:
The aim of the work was to determine how the induction of androgenesis in selected genotypes of Salix viminalis is affected by thermal factors and medium composition. Anthers isolated from male clones of S. viminalis were pre-cultured at 4, 27 and 32°C for two to eight days in liquid Kyo medium with and without the addition of mannitol and on solid MS or WPM medium. The solid media were supplemented with different concentration of disaccharidesand various combinations of growth regulators including kinetin, 2iP, IAA and IBA. Multi-nucleate microspores indicative of androgenesis were observed in anthers pre-cultured for seven days at 4°C in liquid Kyo medium containing mannitol. The rate of androgenesis was higher when the anthers were transferred to solid modified MS medium containing high concentrations of sucrose and kinetin. In studied genotypes of basket willow early uninucleate microspores underwent sporophytic divisions. Ultrastructural observations showed differences in cellular arrangement of pre-stressed microspores. In Salix viminalis, microspores without starch grains and decreased number of lipid bodies were potentially androgenic cells.
Źródło:
Dendrobiology; 2009, 61; 55-64
1641-1307
Pojawia się w:
Dendrobiology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Applications of recent advances at the Institute of Grassland and Environmental Research in cytogenetics of the Lolium-Festuca complex
Autorzy:
Humphreys, M W
Thomas, H M
King, I P
Morgan, W G
Meredith, M R
Harper, J A
Humphreys, M O
Bettany, A J E
Dalton, S J
James, A R
Ougham, H J
Thomas, H
Powiązania:
https://bibliotekanauki.pl/articles/2046675.pdf
Data publikacji:
1997
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
RNA
in situ
tissue culture
gene transfer
chromosome segment
anther culture
androgenesis
plant breeding
introgression mapping
fluorescence
hybridization
Lolium-Festuca complex
drought resistance
gene isolation
tetraploid hybrid
DNA
meiosis
grass
chromosome behaviour
Opis:
Recent advances at Institute of Grassland and Environmental Research (Aberystwyth, U.K.) in cytogenetics of the Lolium/Festuca complex places us in the advantageous position of being able to map genes of agronomic importance onto chromosome arms using fluorescence in situ hybridization (FISH). The ability to physically map genes leads to the capability for "dissecting" quantitative traits into their different components and will lead to better understanding of the complex physiological processes involved and the identification of their genetic control. By tagging genes of interest, using molecular and morphological markers, it will be possible to select and combine suites of desirable genes in a single genotype and thus produce novel cultivars by conventional breeding procedures. Programmes for introgression depend on the relationships between species and on levels of chromosome pairing. Phylogenetic relationships within the Lolium/Festuca complex are being determined using both genomic in situ hybridization (GISH) and FISH. With recent advances in genetic manipulation within the Lolium/Festuca complex, opportunities now arise for gene transfer from Lolium and Festuca species into other important agricultural crops.
Źródło:
Journal of Applied Genetics; 1997, 38, 3; 273-284
1234-1983
Pojawia się w:
Journal of Applied Genetics
Dostawca treści:
Biblioteka Nauki
Artykuł

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