A comparison of anther culture and maize pollination for haploid production in wheat

The use of 2,4-D as a post pollination treatment to fertilize florets instead of GA₃ provided a two-fold improvement in seed set, culturable embryos and hence green plant production from wheat and maize pollination. The efficiency of the wheat by maize pollination method for haploid production was equal to the anther culture method in F₁ hybrid combinations that were responsive to anther culture. However, in recalcitrant combinations obtained from non-responsive genotypes the maize pollination method was far superior.