Effect of genotype and media composition on embryoid induction and plant regeneration from anther culture in triticale

Anthers of twenty triticale genotypes were cultured on three different media: 1 - PII (Chuang et al. 1978) with increased 2,4-D to 2 mg L⁻¹ and agarose 6 g L⁻¹ , 2 - Macro-, micronutrients and vitamines like in MN6 (Chu, Hill 1988) + 2 mg L⁻¹ 2.4-D + 0.5 mg L⁻¹ KIN + 5 mg L⁻¹ FeEDTA + 90 g L⁻¹ sucrose; 3 - Macro-, micronutrients and vitamines like in MN6 (Chu, Hill, 1988) + 2 mg L⁻¹ 2.4-D + 0.5 mg L⁻¹ KIN + 5 mg L⁻¹ FeEDTA + 120 g L⁻¹ sucrose. Embryoid induction and plant regeneration were influenced by donor plant genotype and induction medium. Medium 1 was the best for embryoid induction, while for green plant regeneration the best were media 1 and 2. Out of 300 anthers from each genotype plated on each of the three media, 64-1250, 12-486 and 6-212 somatic embryos and 8-86, 3-136 and 1-26 green plants were recorded, on media 1, 2 and 3, respectively.