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Wyszukujesz frazę "Enzyme" wg kryterium: Temat


Tytuł:
The effect of pulsation magnetic field on enzymatic activity of hydrolases of Candida albicans strains isolated from patients of risk group
Autorzy:
Glowacka, A.
Budak, A.
Powiązania:
https://bibliotekanauki.pl/articles/836784.pdf
Data publikacji:
1998
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
patient
hydrolytic enzyme
enzyme
enzyme activity
Candida albicans
Źródło:
Annals of Parasitology; 1998, 44, 3
0043-5163
Pojawia się w:
Annals of Parasitology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
An easy procedure to transform the ratio of two polynomials of first degree into Michaelis-Menten-type equations. Application to the ordered Uni Bi enzyme mechanism.
Autorzy:
Fontes, Rui
Ribeiro, João
Sillero, Antonio
Powiązania:
https://bibliotekanauki.pl/articles/1044439.pdf
Data publikacji:
2000
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
rectangular hyperbolas
enzyme inhibition
enzyme equations
enzyme kinetics
graphical presentations
Opis:
It is not always clear that some equations affected by complicated factors can, actually, be interpreted as a ratio of two polynomials of first degree and so that they can be, in general, represented by rectangular hyperbolas. In this paper we present an easy procedure to rearrange those equations into Michaelis-Menten-type equations and so to make the aspects of these rectangular hyperbolas more clear, particularly for researchers familiar with general biochemistry. As an example, the method is applied to transform the classical rate equation of the Cleland×s Ordered Uni Bi enzyme mechanism.
Źródło:
Acta Biochimica Polonica; 2000, 47, 1; 259-268
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Inhibition and activation of enzymes. The effect of a modifier on the reaction rate and on kinetic parameters.
Autorzy:
Fontes, Rui
Ribeiro, João
Sillero, Antonio
Powiązania:
https://bibliotekanauki.pl/articles/1044438.pdf
Data publikacji:
2000
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
enzyme inhibition
enzyme modifier
rapid equilibrium kinetics
I50
enzyme activation
enzyme kinetics
graphical presentations
Opis:
A combined analysis of enzyme inhibition and activation is presented, based on a rapid equilibrium model assumption in which one molecule of enzyme binds one molecule of substrate (S) and/or one molecule of a modifier X. The modifier acts as activator (essential or non-essential), as inhibitor (total or partial), or has no effect on the reaction rate (v), depending on the values of the equilibrium constants, the rate constants of the limiting velocity steps, and the concentration of substrate ([S]). Different possibilities have been analyzed from an equation written to emphasize that v = Ł([X]) is, in general and at a fixed [S], a hyperbolic function. Formulas for Su (the value of [S], different from zero, at which v is unaffected by the modifier) and vsu (v at that particular [S]) were deduced. In Lineweaver-Burk plots, the straight lines related to different [X] generally cross in a point (P) with coordinates (Su, vsu). In certain cases, point P is located in the first quadrant which implies that X acts as activator, as inhibitor, or has no effect, depending on [S]. Furthermore, we discuss: (1) the apparent Vmax and Km displayed by the enzyme in different situations; (2) the degree of effect (inhibition or activation) observed at different concentrations of substrate and modifier; (3) the concept of Ke, a parameter that depends on the concentration of substrate and helps to evaluate the effect of the modifier: it equals the value of [X] at which the increase or decrease in the reaction rate is half of that achieved at saturating [X]. Equations were deduced for the general case and for particular situations, and used to obtain computer-drawn graphs that are presented and discussed. Formulas for apparent Vmax, Km and Ke have been written in a way making it evident that these parameters can be expressed as pondered means.
Źródło:
Acta Biochimica Polonica; 2000, 47, 1; 233-257
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Novel potential of pancreatic-like enzymes of microbial origin in exocrine pancreatic insufficiency - study on a pig model
Autorzy:
Szwiec, K.
Goncharova, K.
Velverde-Piedra, J.L.
Ushakova, G.
Kovalenko, T.
Osadchenko, I.
Kardas, M.
Swieboda, P.
Podgurniak, P.
Winiarczyk, M.
Grochowska-Niedworok, E.
Lozinska, L.
Filip, R.
Pierzynowski, S.G.
Powiązania:
https://bibliotekanauki.pl/articles/3181.pdf
Data publikacji:
2015
Wydawca:
Instytut Medycyny Wsi
Tematy:
pancreatic-like enzyme
microbial enzyme
exocrine pancreatic insufficiency
porcine model
pig
animal model
enzyme activity
Opis:
Introduction. The standard porcine-derived pancreatic enzyme replacement therapy (PERT) is a lifesaving treatment for patients with diseases causing exocrine pancreatic insufficiency (EPI). An attempt to replace PERT with microbial enzymes were undertaken. The aim was to highlight whether the mode of application, mixed with food or applied directly to the stomach, of pancreatic-like enzymes of microbial origin (PLEM) can affect their activity along the gastrointestinal tract. Materials and method. The activity of amylase, lipase and proteinase in the stomach, duodenum and ileum were tested in EPI pigs (n=6) after supplementation of PLEM, either orally – before and during feed consumption – or via the stomach – before and during feed consumption. Healthy pigs not treated with PLEM (n=3) served as controls. Activity of the enzymes measured in the chyme were obtained together with the digesta pH. Activity of the enzymatic residues in the stool samples was also checked. Results. The highest pancreatic enzyme activities were found in the duodenum of the healthy pigs (amylase 162,68 kU/ mL, lipase 507,34 kU/mL and protolitic (trypsin) activity 357,60 kU/mL). Nevertheless, the microbial enzymes remained also active along the entire length of the GIT – including stomach in EPI pigs, regardless of their route of administration. However, activity level was significantly lower. Discussion. Results indicate that the activity pattern of PLEM in the small intestine mimics the activity of the natural endogenous pancreatic enzymes in healthy pigs. The most physiological features of PLEM were observed when enzymes were offered orally. The magnitude of PLEM activity in the stomach of EPI pigs was essential and significantly higher than that measured in healthy pigs, thus being somewhat not physiological, and for health reasons of the patients should be further explored. Interestingly, specific trypsin-like activity was measured in all parts of the GIT after PLEM application. However, proteolytic activity of the experimental proteaze in in vitro studies did not exhibit trypsin-like activity.
Źródło:
Journal of Pre-Clinical and Clinical Research; 2015, 09, 1
1898-2395
Pojawia się w:
Journal of Pre-Clinical and Clinical Research
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Influence of the zearalenone on the activity of chosen liver enzymes in a rat
Autorzy:
Stadnik, A
Borzecki, A.
Powiązania:
https://bibliotekanauki.pl/articles/51110.pdf
Data publikacji:
2009
Wydawca:
Instytut Medycyny Wsi
Tematy:
rat
liver
enzyme
enzyme activity
zearalenone
mycotoxin
Fusarium
liver cell
Źródło:
Annals of Agricultural and Environmental Medicine; 2009, 16, 1; 31-35
1232-1966
Pojawia się w:
Annals of Agricultural and Environmental Medicine
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Histochemical study on oxidative and antioxidative enzymes of mouse liver in the course of infection with Babesia microti
Autorzy:
Kuzna-Grygiel, W.
Kolodziejczyk, L.
Powiązania:
https://bibliotekanauki.pl/articles/838274.pdf
Data publikacji:
1998
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
parasite
oxidative enzyme
infection
mouse
Babesia microti
mice
liver
antioxidative enzyme
Źródło:
Annals of Parasitology; 1998, 44, 3
0043-5163
Pojawia się w:
Annals of Parasitology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
L-asparaginase: An ultimate anti-neoplastic enzyme
Autorzy:
Prasad Talluri, V.S.S.L.
Bhavana, M.
Mahesh Kumar, M.V.S.
Rajagopal, S.V.
Powiązania:
https://bibliotekanauki.pl/articles/11250.pdf
Data publikacji:
2014
Wydawca:
Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
Tematy:
L-asparaginase
anti-neoplastic enzyme
enzyme
acute lymphoblastic leukemia
chemotherapeutic drug
Opis:
The objective of the study described the importance of L-asparaginase and its importance in the field of medicine. Different types of enzymes are produced based on the adaptation to the environment where the living organisms live to tune the metabolic pathways according to their adapted changes. The enzymes present in various organs are produced by many cell types in multicellular organisms. Except ribosomes all other known enzymes are proteinaceous in nature. L-asparaginase is a potential therapeutic agent for acute lymphoblastic leukemia (ALL) and chronic myelogenous leukemia which is approved by FDA & WHO. L-asparaginase catalyzes the deamination of L-asparagine to L-aspartic acid & ammonia. Unlike normal cells, malignant cells require large amount of L-asparagine for protein synthesis and cell division. From this background the present review is an effort to gather the information on the mechanism, sources, molecular details and application of L-asparaginase enzyme.
Źródło:
International Letters of Natural Sciences; 2014, 10
2300-9675
Pojawia się w:
International Letters of Natural Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Usability of enzyme activity in estimation of forest soil quality
Autorzy:
Blonska, E.
Januszek, K.
Powiązania:
https://bibliotekanauki.pl/articles/38481.pdf
Data publikacji:
2013
Wydawca:
Instytut Badawczy Leśnictwa
Tematy:
usability
enzyme activity
estimation
forest soil
soil quality
soil enzyme
site index
Opis:
Taking into account the progressive degradation of soils it is important to assess their quality. Soil quality depends on a large number of physical, chemical, biological and biochemical properties. In the publications available, there are presented three approaches regarding the use of soil properties to estimate soil quality: (1) the use of individual properties, (2) the use of simple indexes and (3) the use of complex indexes derived from combinations of different properties. The purpose of this study was to investigate the possibility to use enzymes as indicators of forest soil quality. Experimental plots (43) were located in central Poland. The study was carried out in a number of diverse fresh forest sites. To assess the quality of forest soils dehydrogenase and urease activity and the degree of base saturation were used. One of the final conclusions point out that enzymatic activity indicates current site condition as well as the changes that occur in soil better than soil physical and chemical properties. In other words, in comparison to soil enzymatic activity, soil physico-chemical properties constitute a less sensitive indicator of soil changes.
Źródło:
Folia Forestalia Polonica. Series A . Forestry; 2013, 55, 1
0071-6677
Pojawia się w:
Folia Forestalia Polonica. Series A . Forestry
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Some specific and non-specific phosphatases in the sporocyst of Fasciola hepatica. 3. Enzymes associated with glycolysis
NIEKTÓRE SPECYFICZNE I NIESPECYFICZNE FOSFATAZY W SPOROCYSTACH FASCIOLA HEPATICA. III. ENZYMY ZWIĄZANE Z GLIKOLIZĄ
Autorzy:
Humiczewska, M.
Powiązania:
https://bibliotekanauki.pl/articles/2148926.pdf
Data publikacji:
1997
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
glucose-6-phosphatase
aldolase
enzyme
sporocyst
glycolytic enzyme
Fasciola hepatica
phosphatase
hexokinase
Opis:
Using histochemical and cytophotometric methods, enzymes associated with anaerobie glycolysis: glucose-6-phosphatase, hexokinase, and aldolase in the developing sporocysts of Fasciola hepatica were studied. Highest activity of these enzymes was found in the germ balls in the sporocysts, at all phases of their development, which is related to intensive proliferation and differentiation of the embryos developing inside the germ balls.
Źródło:
Wiadomości Parazytologiczne; 1997, 43, 4; 411-423
0043-5163
Pojawia się w:
Wiadomości Parazytologiczne
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Proteins and enzymatic systems in three varieties of Helianthus annuus L.
Autorzy:
Kalinowski, A
Klimko, M.
He, L.
Kluza, M.
Powiązania:
https://bibliotekanauki.pl/articles/878224.pdf
Data publikacji:
2001
Wydawca:
Uniwersytet Przyrodniczy w Poznaniu
Tematy:
variability
Helianthus annuus
enzyme
hybrid
electrophoresis
protein
enzyme system
oil plant
isoelectric focusing
Opis:
The paper reports results of analysis of protein and eight enzymatic systems of three varieties of Helianthus annuus L. (Wielkopolski, Coril, and Frankasol) performed by three electrophoretic methods. Electrophoretic analyses allowed finding protein and isoenzymes markers for each of variety. The two interline hybrids Coril and Frankasol shared more features in common than the population variety Wielkopolski with each of the former. Mean values of the polymorphism index for the protein and the eight enzymes indicate that the variety Wielkopolski is more homogeneous than Coril and Frankasol.
W pracy przedstawiono wyniki analiz białek i ośmiu układów enzymatycznych, rozdzielanych trzema metodami elektroforezy, u trzech odmian Helianthus annuus L. (Wielkopolski, Coril i Frankasol). Elektroforetyczne analizy umożliwiły znalezienie białkowych i izoenzymatycznych markerów dla trzech odmian. U dwóch odmian międzyliniowych mieszańców Coril i Frankasol wykryto więcej cech wspólnych niQ u wymienionych odmian i odmiany Wielkopolski. Średnie wartości indeksu polimorfizmu, obliczone dla białek i ośmiu układów enzymatycznych, pokazały, że odmiana Wielkopolski była bardziej wyrównana niż odmiany Coril i Frankasol.
Źródło:
Roczniki Akademii Rolniczej w Poznaniu. Botanika; 2001, 04
1508-9193
Pojawia się w:
Roczniki Akademii Rolniczej w Poznaniu. Botanika
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The effect of Fe-deficiency on antioxidant enzymes’ activity and lipid peroxidation in wheat leaves
Wpływ deficytu Fe na aktywność enzymów antyoksydacyjnych i peroksydację tłuszczu w liściach pszenicy
Autorzy:
Esfandiari, E.
Sabaghnia, N.
Powiązania:
https://bibliotekanauki.pl/articles/11236546.pdf
Data publikacji:
2012
Wydawca:
Uniwersytet Przyrodniczy w Lublinie. Wydawnictwo Uniwersytetu Przyrodniczego w Lublinie
Tematy:
iron deficiency
antioxidative enzyme
enzyme activity
lipid peroxidation
wheat
leaf
catalase
superoxide dismutase
Źródło:
Annales Universitatis Mariae Curie-Skłodowska. Sectio E. Agricultura; 2012, 67, 4; 25-34
0365-1118
Pojawia się w:
Annales Universitatis Mariae Curie-Skłodowska. Sectio E. Agricultura
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Aktywnosc ureazy w wybranych glebach Polski
Urease activity in selected polish soils
Autorzy:
Stepniewska, Z
Samborska, A.
Powiązania:
https://bibliotekanauki.pl/articles/1630458.pdf
Data publikacji:
1999
Wydawca:
Polska Akademia Nauk. Instytut Agrofizyki PAN
Tematy:
Polska
gleby
enzymy
enzymy glebowe
ureaza
aktywnosc ureazowa
aktywnosc enzymatyczna
oznaczanie
Polska
soil
enzyme
soil enzyme
urease
urease activity
enzyme activity
determination
Opis:
Celem badań było określenie aktywności ureazy na wybranych glebach Polski: bielicowych i brunatnych wytworzonych z piasków i glin, lessów, czarnych ziem i mad rzecznych. Badaniom poddano próbki z poziomu 0-10 cm, które pobrano z Banku Gleb Instytutu Agrofizyki PAN. Aktywność ureazy zawierała się w przedziale 0,3256 - 1,7896 pM N-NH4 kg-lh-1. Najniższą aktywność ureazową zanotowano w glebach bielicowych i wynosiła ona 0,33 pM N-NH4 kg-lh-1. W glebach brunatnych wytworzonych z piasków i lessów aktywność ureazy wahała się w granicach 0,55 - 1,00 pM N-NH4 kg-lh-1. Najwyższą aktywność stwierdzono w glebach: czarnych ziemiach, madach rzecznych, glebach murszowych i murszowatych 1,43 - 1,79 pM N-NH4 kg-lh-1. W oparciu o analizę statystyczną otrzymanych wyników można stwierdzić, że aktywność ureazowa pozostaje w ścisłym związku z zawartością materii organicznej.
The aim of the investigation was to measure activity of urease in different polish soils such as podzolic and brown developed from sands and loams, brown developed from loess, as well as in black earths and aluvials soils. The measurements were performed on samples taken from 0-10 cm depths. The urease activity was in the range of 0,3256 - 1,7896 pM N-NH4+ kg''h‘'. The lowest activity was noted in the podzolic soil and was on the level of 0,3256 pM N-NH4+ kg’'h''. In the brown and lessive soils the urease activity was in the rangę 0,55-1,00 pM N-NH4+ kg''h_l. The highest urease activity were found in black earths, alluvials soils, Eutric Histosol Soils 1,43-1,79 pM N-NH/kg1 h’’. The results obtained allow to conclude that the urease activity is tightly connected with the organie matter content of the soil.
Źródło:
Acta Agrophysica; 1999, 22; 219-228
1234-4125
Pojawia się w:
Acta Agrophysica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Activity of respiratory enzymes in embryogenesis of Toxocara canis
Autorzy:
Kolodziejczyk, L.
Powiązania:
https://bibliotekanauki.pl/articles/836286.pdf
Data publikacji:
1998
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
red fox
parasite
respiratory enzyme
carnivorous mammal
enzyme
dog
embryogenesis
Toxocara canis
fox
egg
Źródło:
Annals of Parasitology; 1998, 44, 3
0043-5163
Pojawia się w:
Annals of Parasitology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
AtDeg2 - a chloroplast protein with dual protease/chaperone activity
Autorzy:
Jagodzik, P.
Adamiec, M.
Jackowski, G.
Powiązania:
https://bibliotekanauki.pl/articles/57301.pdf
Data publikacji:
2014
Wydawca:
Polskie Towarzystwo Botaniczne
Tematy:
chloroplast protein
protease
proteolytic enzyme
chaperone
enzyme activity
chloroplast
PDZ domain
Opis:
Chloroplast protease AtDeg2 (an ATP-independent serine endopeptidase) is cytosolically synthesized as a precursor, which is imported into the chloroplast stroma and deprived of its transit peptide. Then the mature protein undergoes routing to its functional location at the stromal side of thylakoid membrane. In its linear structure AtDeg2 molecule contains the protease domain with catalytic triad (HDS) and two PDZ domains (PDZ1 and PDZ2). In vivo AtDeg2 most probably exists as a supposedly inactive haxamer, which may change its oligomeric stage to form active 12-mer, or 24-mer. AtDeg2 has recently been demonstrated to exhibit dual protease/chaperone function. This review is focused on the current awareness with regard to AtDeg2 structure and functional significance.
Źródło:
Acta Societatis Botanicorum Poloniae; 2014, 83, 3
0001-6977
2083-9480
Pojawia się w:
Acta Societatis Botanicorum Poloniae
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Composites of polyvinyl alcohol (PVA) hydrogel and calcium and magnesium phosphate formed by enzymatic functionalization
Autorzy:
Douglas, T. E. L.
Piegat, A.
Schaubroeck, D.
El Fray, M.
Dubruel, P.
Powiązania:
https://bibliotekanauki.pl/articles/285623.pdf
Data publikacji:
2012
Wydawca:
Akademia Górniczo-Hutnicza im. Stanisława Staszica w Krakowie. Polskie Towarzystwo Biominerałów
Tematy:
hydrogel
composite
mineralization
enzyme
Opis:
In this study, polyvinyl alcohol (PVA) hydrogels designed for osteochondral regeneration were mineralized with calcium phosphate (CaP) and magnesium phosphate (MgP) by incorporation of the enzyme alkaline phosphatase (ALP) and incubation in solutions of 0.1 M calcium or magnesium glycerophosphate (CaGP, MgGP). Hydrogels incubated in water served as controls. Dry mass percentage, i.e. the percentage consisting of polymer and mineral and not water, was markedly higher for samples incubated in CaGP and MgGP and decreased in the order: CaGP>MgGP>water. CaP and MgP formation was confirmed by FTIR. SEM revealed spherical deposits characteristic for apatite in samples incubated in CaGP and plate-like crystals of the magnesium phosphate bobierrite in samples incubated in MgGP, while samples incubated in water were devoid of deposits. Rheometric testing showed that storage modulus of hydrogels incubated in CaGP and MgGP was markedly higher and decreased in the order: CaGP>MgGP>water. The results prove the principle of enzymatic mineralization of PVA with CaP and MgP to improve their suitability for bone regeneration applications.
Źródło:
Engineering of Biomaterials; 2012, 15, no. 116-117 spec. iss.; 137-138
1429-7248
Pojawia się w:
Engineering of Biomaterials
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Influence of selenium on growth, lipid peroxidation and antioxidative enzyme activity in melon (Cucumis melo L.) seedlings under salt stress
Autorzy:
Hu, K.L.
Zhang, L.
Wang, J.T.
You, Y.
Powiązania:
https://bibliotekanauki.pl/articles/58727.pdf
Data publikacji:
2013
Wydawca:
Polskie Towarzystwo Botaniczne
Tematy:
selenium
plant growth
lipid peroxidation
antioxidative enzyme
enzyme activity
melon
Cucumis melo
seedling
salt stress
Opis:
The objective of this study was to investigate the effect of exogenous selenium (Se) supply (0, 2, 4, 8, 16 μM) on the growth, lipid peroxidation and antioxidative enzyme activity of 100 mM NaCl-stressed melon (Cucumis melo L.) seedlings. Salt stress significantly reduced the growth attributes including stem length, stem diameter, dry weight and increased antioxidative enzyme activity [superoxide dismutase (SOD), peroxidase (POD), catalase (CAT)]. Moreover, the plant exhibited a significant increase in electrolyte leakage and malondialdehyde (MDA) content under NaCl stress. Se supplementation not only improved the growth parameters but also successfully ameliorated the adverse effect caused by salt stress in melon seedlings. However, the mitigation of NaCl-stressed seedlings was different depending on the Se concentration. At lower concentrations (2–8 μM), Se improved growth and acted as antioxidant by inhibiting lipid peroxidation and increasing in SOD and POD enzymes activity under salt stress. At higher concentrations (16 μM), Se exerted diminished beneficial effects on growth. Whereas CAT activity was enhanced. The result indicated that Se supplementation had a positive physiological effect on the growth and development of salt-stressed melon seedlings.
Źródło:
Acta Societatis Botanicorum Poloniae; 2013, 82, 3
0001-6977
2083-9480
Pojawia się w:
Acta Societatis Botanicorum Poloniae
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Some specific and non-specific phosphatases in the sporocyst of Fasciola hepatica. 3. Enzymes associated with glycolysis
Autorzy:
Humiczewska, M
Powiązania:
https://bibliotekanauki.pl/articles/837871.pdf
Data publikacji:
1997
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
glucose-6-phosphatase
aldolase
enzyme
sporocyst
glycolytic enzyme
Fasciola hepatica
phosphatase
hexokinase
Opis:
Using histochemical and cytophotometric methods, enzymes associated with anaerobie glycolysis: glucose-6-phosphatase, hexokinase, and aldolase in the developing sporocysts of Fasciola hepatica were studied. Highest activity of these enzymes was found in the germ balls in the sporocysts, at all phases of their development, which is related to intensive proliferation and differentiation of the embryos developing inside the germ balls.
Źródło:
Annals of Parasitology; 1997, 43, 4
0043-5163
Pojawia się w:
Annals of Parasitology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The role of boron in animal health
Autorzy:
Kabu, M.
Uyarlar, C.
Zarczynska, K.
Milewska, W.
Sobiech, P.
Powiązania:
https://bibliotekanauki.pl/articles/961611.pdf
Data publikacji:
2015
Wydawca:
Uniwersytet Warmińsko-Mazurski w Olsztynie / Polskie Towarzystwo Magnezologiczne im. Prof. Juliana Aleksandrowicza
Tematy:
boron
enzyme
hormone
mineral
animal health
Opis:
Boron is a mineral used for various purposes in glass, ceramics, automotive and paint industries. Recently, boron has been confirmed to be an essential element for plants, animals and humans, although the knowledge of its biological effects is rather scanty. Boron is a dynamic trace element, and inorganic borates are transformed into boric acids and absorbed from mucosal surfaces, even when they are in low levels of physiological pH. It has been determined that boron affects many enzymes, bone development, mineralization, Ca, P, Mg and energy metabolism. Boron mineral compounds can be effective in optimizing the performance of an organism, treatment of bone structure disorders, reduction of cholesterol and triglyceride levels. Beside the effects it produces specifically on fat and lipid metabolisms, boron can influence the activity of vitamin D and affect some disorders connected with its deficiency. Although several studies on effects of boron on some mechanisms have been conducted over the last ten years, the available information remains insufficient.
Źródło:
Journal of Elementology; 2015, 20, 2
1644-2296
Pojawia się w:
Journal of Elementology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Theoretical model explaining the relationship between the molecular mass and the activation energy of the enzyme revealed by a large-scale analysis of bioinformatics data
Autorzy:
Pawlowski, Piotr
Zielenkiewicz, Piotr
Powiązania:
https://bibliotekanauki.pl/articles/1039585.pdf
Data publikacji:
2013
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
statistics
catalysis
turnover
enzyme
molecular mass
Opis:
A general dependence of the enzyme catalytic rate on its mass was revealed when a statistical analysis of 17065 records from the EMP database was performed. The estimated activation energy of the catalytic process decreases asymptotically with the enzyme molecular mass increase. The proposed theoretical model postulates the existence of an intermediate complex of the enzyme and the departing product. It allows for the explanation of the discovered mass-energy relationship, as an effect of the global enzyme-product interactions during complex dissociation. Fitted parameters of the model seem to be in agreement with those widely accepted for the van der Waals energy of molecular interactions. Their values also agree with the picture of the hydrogen bonding in the catalytic process and suggest that surface walk can be the favorable way of the product departure.
Źródło:
Acta Biochimica Polonica; 2013, 60, 2; 239-247
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Biochemical study of trehalase activity in Aspergillus niger conidia
Autorzy:
Chrungu, Deepti
Sharma, Sukesh Chander
Akhtar, Mahmood
Powiązania:
https://bibliotekanauki.pl/articles/1178299.pdf
Data publikacji:
2017
Wydawca:
Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
Tematy:
Aspergillus niger
Conidia
Enzyme Activity
Trehalase
Opis:
Trehalase (α, α-trehalose-1-C-glucohydrolase, EC 3.2.1.28) is a glycoside hydrolase enzyme that catalyzes the conversion of trehalose to glucose. Trehalose hydrolysis by trehalase enzyme is an important physiological process for various organisms, such as fungal spore germination, insect flight, and the resumption of growth in resting cells. So by studying biochemical properties of trehalase we can control the spore germination of harmful fungus, Apergillus niger. Till now trehalase has been reported from many other organisms including plants and animals, Trehalase has been purified and characterized from various organisms such as Saccharomyces cerevisiae, Lentinula edodes, Acidobacterium capsulatum, and Medicago sativa, as well as root nodules of Phaseolus vulgaris, soybean and from the from the seeds of Triticum aestivum. In this study, trehalase was purified from Aspergillus niger grown on cellulosic medium. And this purified trehalase was tested for various requirements it may need for enzymatic activity such as inorganic phosphate and Magnesium ions (Mg2+).
Źródło:
World Scientific News; 2017, 86, 3; 349-356
2392-2192
Pojawia się w:
World Scientific News
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Lipaza - charakterystyka, zastosowanie, sposoby immobilizacji
Lipase - characterization, applications and methods of immobilization
Autorzy:
Lasoń, E.
Ogonowski, J.
Powiązania:
https://bibliotekanauki.pl/articles/1287339.pdf
Data publikacji:
2010
Wydawca:
Stowarzyszenie Inżynierów i Techników Przemysłu Chemicznego. Zakład Wydawniczy CHEMPRESS-SITPChem
Tematy:
lipaza
immobilizacja
enzym
lipase
immobilization
enzyme
Opis:
W pracy przedstawiono charakterystykę enzymu lipazy ze szczególnym uwzględnieniem jego otrzymywania, właściwości katalitycznych, które decydują o szerokich możliwościach zastosowania oraz metod jego immobilizacji.
In this paper the characterization of the lipase enzyme was presented, with special consideration to its production and catalytic properties which decide about the wide-ranging applications, as well as about the methods of its immobilization.
Źródło:
Chemik; 2010, 64, 2; 97-102
0009-2886
Pojawia się w:
Chemik
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Hydroxyapatite as a support in protease immobilization process
Autorzy:
Zdarta, J.
Budzinska, K.
Kolodziejczak-Radzimska, A.
Klapiszewski, Ł.
Siwinska-Stefanska, K.
Bartczak, P.
Piasecki, A.
Maciejewski, H.
Jesionowski, T.
Powiązania:
https://bibliotekanauki.pl/articles/110452.pdf
Data publikacji:
2015
Wydawca:
Politechnika Wrocławska. Oficyna Wydawnicza Politechniki Wrocławskiej
Tematy:
hydroxyapatite
enzyme immobilization
protease
physicochemical characteristic
Opis:
Hydroxyapatite is used as a matrix for immobilization of protease from Aspergillus oryzae by a process of adsorption. The matrix obtained has the surface area of 26 m2/g and particles in the shape of flakes of diameters no greater than 650 nm. The efficiency of the proposed method was confirmed by the Fourier transform infrared spectroscopy, elemental analysis and by analysis of parameters of the pore structure of matrix and products after immobilization. On the basis of the Bradford method it was found that the greatest amount of enzyme (132 mg/g) was immobilized from a solution of initial enzyme concentration of 7 mg/cm3 after 24 h of the process.
Źródło:
Physicochemical Problems of Mineral Processing; 2015, 51, 2; 633-646
1643-1049
2084-4735
Pojawia się w:
Physicochemical Problems of Mineral Processing
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Change in hydrolytic enzyme efficiency over time
Autorzy:
Szadkowska, Dominika
Powiązania:
https://bibliotekanauki.pl/articles/2200102.pdf
Data publikacji:
2020
Wydawca:
Szkoła Główna Gospodarstwa Wiejskiego w Warszawie. Wydawnictwo Szkoły Głównej Gospodarstwa Wiejskiego w Warszawie
Tematy:
enzymatic hydrolysis
enzyme
poplar wood
holocellulose
Opis:
Change in hydrolytic enzyme efficiency over time. The purpose of this study was to determine the action of hydrolytic enzymes (by Dyadic Cellulase CP CONC, and the Dyadic Xylanase 2 XP CONC) over time. Chromatographic analysis of holocellulose samples subjected to enzymatic hydrolysis was performed. The following hydrolysis parameters were used: time 48h, temperature 45 ⁰C, acetate buffer pH 5.4, commercial enzymes Dyadic. Holocellulose extracted by the sodium chlorite method from white poplar wood (Populus alba L.) was used. The final yield of enzymatic hydrolysis was determined. The results of hydrolysis performed at intervals were compared. The results obtained show that the hydrolysis yield of holocellulose after five months decreased by 40 p.p. for glucose yield and by 25 p.p. for xylose yield. The yield for glucose after two and a half years decreases by 68 p.p. and 62 p.p. for xylose compared to the initial yield.
Zmiana wydajności enzymów hydrolitycznych w czasie Celem pracy było określenie działania enzymów hydrolitycznych w czasie. Przeprowadzono analizę chromatograficzną próbek holocelulozy poddanych hydrolizie enzymatycznej. Zastosowano następujące parametry hydrolizy: czas 48h, temperatura 45 ⁰C, bufor octanowy pH 5,4, enzymy komercyjne Dyadic. Zastosowano holocelulozę pozyskaną metodą chlorynu sodowego z drewna topoli bialej (Populs alba L.). Określono końcową wydajność hydrolizy enzymatycznej. Porównano wyniki hydrolizy przeprowadzonej w odstępach czasu. Uzyskane wyniki wskazują, że wydajność hydrolizy holocelulozy po pięciu miesiącach zmniejszyła się o 40 p.p. dla wydajności glukozy i o 25 p.p. dla wydajności ksylozy. Wydajność glukozy po dwóch i pół roku obniża się o 68 p.p., a ksylozy o 62 p.p. w stosunku do wydajności początkowej.
Źródło:
Annals of Warsaw University of Life Sciences - SGGW. Forestry and Wood Technology; 2020, 112; 79--84
1898-5912
Pojawia się w:
Annals of Warsaw University of Life Sciences - SGGW. Forestry and Wood Technology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The effects of nitric oxide on some antioxidant enzyme activities under salt stress in sunflower plants
Autorzy:
Yurekli, F.
Kirecci, O.A.
Celik, I.
Powiązania:
https://bibliotekanauki.pl/articles/12314257.pdf
Data publikacji:
2019
Wydawca:
Uniwersytet Przyrodniczy w Lublinie. Wydawnictwo Uniwersytetu Przyrodniczego w Lublinie
Tematy:
plant cultivation
common sunflower
Helianthus annuus
nitric oxide
antioxidant enzyme
antioxidant activity
enzyme activity
salt stress
Opis:
The effects of externally applied sodium nitroprusside on superoxide dismutase (SOD), glutathione peroxidase (GPx), ascorbate peroxidase (APX), and catalase (CAT) antioxidant enzyme activities, nitric oxide (NO) levels have been investigated in salt stress resistant and sensitive sunflower plants. NaCl treatments and SNP treatments simultaneously with salt application (NaCl+SNP) were performed. SOD, GPx, APX and CAT antioxidant enzyme activities and NO levels showed differences in leaf tissues treated with 100 µM SNP, different concentrations of NaCl, and NaCl+SNP. SOD, GPx and APX enzyme activities were generally increased in sensitive variety, but decreased in tolerant variety. However, while general increase in CAT enzyme activity was determined in tolerant type, a reduction was established in sensitive type. An increase was determined in both types in NO levels. It is evident from these results that administration of NO donor SNP can cope with reactive oxygen species in both varieties. This study indicates that negative effects of salt stress on different sun flower varieties can be recovered by nitric oxide.
Źródło:
Acta Scientiarum Polonorum. Hortorum Cultus; 2019, 18, 5; 171-179
1644-0692
Pojawia się w:
Acta Scientiarum Polonorum. Hortorum Cultus
Dostawca treści:
Biblioteka Nauki
Artykuł

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