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Wyświetlanie 1-45 z 45
Tytuł:
Indicated mean effective pressure oscillations in a natural gas combustion engine by recurrence plots
Analiza wykresów rekurencyjnych dla średniego efektywnego ciśnienia indykowanego w silniku spalinowym zasilanym gazem naturalnym
Autorzy:
Litak, G.
Syta, A.
Yao, B. -F.
Li, G.-X
Powiązania:
https://bibliotekanauki.pl/articles/280206.pdf
Data publikacji:
2009
Wydawca:
Polskie Towarzystwo Mechaniki Teoretycznej i Stosowanej
Tematy:
engine
combustion
recurrence plot
nonlinear oscillations
Opis:
We investigate the nonlinear time series of Indicated Mean Effective Pressure (IMEP) of the spark ignition engine cyclic combustion process of a natural gas. By applying the embedding theorem and the recurrence plots technique, we show changes in the engine dynamics for different equivalence ratios. Especially, we provide arguments for intermittency behaviour.
Zbadano nieliniowe przebiegi czasowe Średniego Ciśnienia Indykowanego cyklicznego procesu spalania w silniku spalinowym zasilanym gazem naturalnym. Stosując twierdzenie o zanurzeniu oraz technikę wykresów rekurencyjnych, pokazano istotne różnice w dynamice silnika przy zmianie składu mieszanki paliwa wyrażonej wartością współczynnika równowagowego. W szczególności podano argumenty przemawiające za występowaniem zjawiska intermitencji.
Źródło:
Journal of Theoretical and Applied Mechanics; 2009, 47, 1; 55-67
1429-2955
Pojawia się w:
Journal of Theoretical and Applied Mechanics
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Dynamic response of a base-isolated concrete rectangular liquid-storage structure under large amplitude sloshing
Dynamiczna odpowiedź prostokątnej struktury magazynowania substancji ciekłych z wykorzystaniem betonu izolacyjnego
Autorzy:
Cheng, X.
Li, D.
Li, P.
Zhang, X.
Li, G.
Powiązania:
https://bibliotekanauki.pl/articles/230249.pdf
Data publikacji:
2017
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
chlupotanie cieczy
amplituda duża
trzęsienie ziemi
struktura prostokątna
substancja ciekła
magazynowanie
nieliniowość
odpowiedź dynamiczna
liquid sloshing
large amplitude
earthquake
rectangular structure
liquid substance
storage
nonlinearity
dynamic response
Opis:
Considering concrete nonlinearity, the wave height limit between small and large amplitude sloshing is defined based on the Bernoulli equation. Based on Navier-Stokes equations, the mathematical model of large amplitude sloshing is established for a Concrete Rectangle Liquid-Storage Structure (CRLSS). The results show that the seismic response of a CRLSS increases with the increase of seismic intensity. Under different seismic fortification intensities, the change in trend of wave height, wallboard displacement, and stress are the same, but the amplitudes are not. The areas of stress concentration appear mainly at the connections between the wallboards, and the connections between the wallboard and the bottom.
Gdy amplituda chlupotania cieczy jest zbliżona do częstotliwości drgań struktury magazynowania substancji ciekłych (CRLSS), wówczas osiągamy rezonans i możemy zaobserwować silne zjawisko nieliniowe. Szkoda jest znacznie większa niż chlupotanie o małej amplitudzie. Obecnie brak jest odpowiedniego raportu na temat badań dynamicznej odpowiedzi struktury magazynowania substancji ciekłych z wykorzystaniem betonu izolacyjnego (CRLSS) z chlupotaniem o dużej amplitudzie, a wpływ materiałów betonowych nie jest brany pod uwagę. W związku z tym, w niniejszej pracy, w oparciu o równanie Bernoulliego, otrzymano ograniczone wysokości fali o dużej amplitudzie chlupotania oraz małej amplitudzie chlupotania. Na podstawie równań Naviera-Stokesa ustanowiono matematyczny model chlupotania o dużej amplitudzie i zbadano odpowiedź sejsmiczną CRLSS podczas chlupotania o dużej amplitudzie. Rozważając równanie Bernoulliego i zadowalający stan, chlupotanie substancji ciekłej jest liniowe, a nieliniowy kwadratowy człon jest lekceważony. W stałym i płynnym interfejsie, struktura magazynowania substancji ciekłych spełnia warunki ciągłości przemieszczania i równowagi siły oddziaływań. Właściwość mechaniczna gumowego zabezpieczenia izolacyjnego została opisana w oparciu o konstytutywną relacje modelu Mooney-Rivlin.
Źródło:
Archives of Civil Engineering; 2017, 63, 1; 33-45
1230-2945
Pojawia się w:
Archives of Civil Engineering
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Health index synthetization and remaining useful life estimation for turbofan engines based on run-to-failure datasets
Synteza wskaźników stanu technicznego oraz ocena pozostałego okresu użytkowania silników turbowentylatorowych z wykorzystaniem zbiorów danych o pracy do czasu uszkodzenia
Autorzy:
Shi, J.
Li, Y.
Wang, G.
Li, X.
Powiązania:
https://bibliotekanauki.pl/articles/301447.pdf
Data publikacji:
2016
Wydawca:
Polska Akademia Nauk. Polskie Naukowo-Techniczne Towarzystwo Eksploatacyjne PAN
Tematy:
hierarchical clustering
relevance vector machine
run-to-failure
remaining useful life
health indices
prognostics
grupowanie hierarchiczne
maszyna wektorów istotnych
praca do czasu uszkodzenia
pozostały okres użytkowania
wskaźnik stanu technicznego
prognostyka
Opis:
Turbofan engines will gradually degrade until failure occurs or life ends if without maintenance. Reliable degradation assessment and remaining useful life (RUL) estimation make sense on both aviation safety and rational maintenance decisions. This paper proposes a data-driven prognostic method on the premise of run-to-failure (RtF) data which are multivariate sensory data collected from the engines operating from normal to failure. After necessary pre-processing to the data, clustering analysis is executed to generate the clusters which represent the multi-states of the degradation process. The failure state cluster is extracted, and then the distance between the pre-processed data and the cluster is calculated. Therefore, one-dimensional time series are generated and defined as the health indices. Afterwards the degradation models are built based on the health indices. Finally, the RUL of a testing unit can be estimated by similarity analysis with the models. Hierarchical clustering (HC) and relevance vector machine (RVM) are the main algorithms employed in this paper. To validate the proposition, a case study is performed on turbofan engines data from Prognostics Center of Excellence (PCoE) at NASA Ames Research Center, and sufficient comparisons were given.
Silniki turbowentylatorowe niepoddane konserwacji ulegają stopniowej degradacji aż do czasu wystąpienia uszkodzenia lub zakończenia cyklu życia. Rzetelna ocena degradacji oraz pozostałego okresu użytkowania (RUL) mają wpływ zarówno na bezpieczeństwo maszyn lotniczych jak i racjonalne podejmowanie decyzji dotyczących utrzymania ruchu. W artykule zaproponowano sterowaną danymi metodę prognostyczną opartą na danych o pracy do czasu uszkodzenia (run-to failure, RTF), które są wielowymiarowymi danymi sensorycznymi zbieranymi podczas normalnej pracy silnika aż do jego uszkodzenia. Po niezbędnej wstępnej obróbce danych, przeprowadzono analizę skupień w celu wygenerowania skupień reprezentujących multi-stany procesu degradacji. Wyodrębniono klaster stanów uszkodzenia, a następnie obliczono odległość między wstępnie przetworzonymi danymi a wyodrębnionym klastrem. Następnie wygenerowano jednowymiarowe szeregi czasowe, które zdefiniowano jako wskaźniki stanu technicznego. Na podstawie tych wskaźników zbudowano modele degradacji. Wreszcie, w oparciu o analizę podobieństwa do opracowanych modeli oceniono RUL jednostki testowej. Główne algorytmy zastosowane w niniejszym opracowaniu to algorytmy grupowania hierarchicznego (HC) oraz maszyny wektorów istotnych (RVM). Aby zweryfikować zaproponowaną w pracy metodę, przeprowadzono studium przypadku z wykorzystaniem danych dot. silników turbowentylatorowych pochodzące z Prognostic Center of Excellence (PCoE) przy NASA Ames Research Center oraz przedstawiono odpowiednie porównania.
Źródło:
Eksploatacja i Niezawodność; 2016, 18, 4; 621-631
1507-2711
Pojawia się w:
Eksploatacja i Niezawodność
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Design of Rascheltronic Vamp Fabric with Double-Colour Pitting Effect
Projektowanie dzianin dwukolorowych z efektem pittingu
Autorzy:
Li, X.
Zhang, A.
Jiang, G.
Powiązania:
https://bibliotekanauki.pl/articles/232311.pdf
Data publikacji:
2017
Wydawca:
Sieć Badawcza Łukasiewicz - Instytut Biopolimerów i Włókien Chemicznych
Tematy:
warp knitting
rascheltronic
vamp fabric
double-colour pitting effect
CAD
projektowanie dzianin dwukolorowych
wspomaganie komputerowe
dzianina
Opis:
Applications of Piezo jacquard and CAD technology in warp-knitting have provided traditional jacquard fabrics with the possibility of innovating the structure design. Research on innovative design and fabrication is conducted aiming at knitting jacquard vamp fabric with the double-colour pitting effect on a technical back. By utilising Piezoelectric jacquard’s performance of displacing both underlapping and overlapping, new structures are formed, such as mesh stitches, koper stitches and float structures. Based on threading with yarns in two colors, jacquard bars in split execution create a pattern with a double-colour effect. To realize a highly efficient design, the paper proposes a computer-aided jacquard design method covering technical parameters and jacquard pattern design modules. Additionally, to pursue convenience and efficiency, mathematic models are built in terms of an automatic borderline design, loop stitching inspection and structure database. The method of jacquard vamp fabric design with a double-color pitting effect has been proven practically by illustrating a vamp design example which meets the requirement of fashion and performance well.
Projektowanie dzianin dwukolorowych z efektem pittingu jest możliwe przy użyciu komputerowego systemu projektowania opartego na specjalnej metodzie dziewiarskiej. Dzięki macierzy i modelowi matematycznemu system obejmuje wszystkie podstawowe parametry techniczne modułów projektowego i kontrolnego oraz modułu bazy danych struktur, które współpracują w celu sprawnego projektowania dzianiny. Na podstawie otrzymanych wyników badań stwierdzono, że wspomagana komputerowo metoda projektowania może być z powodzeniem stosowana w projektowaniu dzianin dwukolorowych z efektem pittingu.
Źródło:
Fibres & Textiles in Eastern Europe; 2017, 3 (123); 92-97
1230-3666
2300-7354
Pojawia się w:
Fibres & Textiles in Eastern Europe
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The Machine Learning Method of PIDVCA
Autorzy:
Li, L.
Wang, X.
Chen, G.
Powiązania:
https://bibliotekanauki.pl/articles/1841618.pdf
Data publikacji:
2020
Wydawca:
Uniwersytet Morski w Gdyni. Wydział Nawigacyjny
Tematy:
learning methods
personified intelligence
collision avoidance
dynamic collision avoidance
static collision avoidance
Opis:
Building a dynamic collision knowledge base of self-learning is one of the core contents of implementing "personified intelligence" in Personifying Intelligent Decision-making for Vessel Collision Avoidance (short for PIDVCA). In the paper, the machine learning method of PIDVCA combined with offline artificial learning and online machine learning is proposed. The static collision avoidance knowledge is acquired through offline artificial learning, and the isomeric knowledge representation integration method with process knowledge as the carrier is established, and the Dynamic collision avoidance knowledge is acquired through online machine learning guided by inference engine. A large number of simulation results show that the dynamic collision avoidance knowledge base constructed by machine learning can achieve the effect of anthropomorphic intelligent collision avoidance. It is verified by examples that the machine learning method of PIDVCA can realize target perception, target cognition and finally obtain an effective collision avoidance decision-making.
Źródło:
TransNav : International Journal on Marine Navigation and Safety of Sea Transportation; 2020, 14, 3; 533-540
2083-6473
2083-6481
Pojawia się w:
TransNav : International Journal on Marine Navigation and Safety of Sea Transportation
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Life assessment of marine ethylene propylene rubber power cables based on hardness retention rate
Autorzy:
Meng, X.
Wang, Z.
Li, G.
Powiązania:
https://bibliotekanauki.pl/articles/141470.pdf
Data publikacji:
2017
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
ethylene propylene rubber (EPR)
nondestructive
hardness retention rate
elongation at break retention rate (EAB %)
termination index
Opis:
The lifetime of ethylene propylene rubber (EPR) insulated cables will decrease because of complex aging processes. From the safety perspective, insulation condition assessment of the cable is essential to maintain an efficient and reliable operation. As a nondestructive and online evaluation method, a hardness retention rate was used to estimate the lifetime of cable. First, accelerated thermal aging tests in the laboratory were performed to measure the elongation at break retention rate (EAB%) and a hardness retention rate at different temperatures. Second, the aging values were processed by the Arrhenius equation and time temperature superposition to assess aging lifetime of insulation at different temperatures and end levels. As the insulation condition assessment of the cable by hardness retention test has no approved standard, the EAB% data were correlated with hardness retention to provide an evaluation basis. The results show that when EAB% picks out the time corresponding to a certain amount of 50% degradation, 10% of hardness retention was chosen as the termination index.
Źródło:
Archives of Electrical Engineering; 2017, 66, 3; 475-484
1427-4221
2300-2506
Pojawia się w:
Archives of Electrical Engineering
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Study on the optimization and performance of TDMA allocation algorithm in ships’ ad hoc networks
Autorzy:
Han, X.
Li, Y.
Liu, G.
Powiązania:
https://bibliotekanauki.pl/articles/259367.pdf
Data publikacji:
2018
Wydawca:
Politechnika Gdańska. Wydział Inżynierii Mechanicznej i Okrętownictwa
Tematy:
timeslot allocation algorithm
ships
ad hoc network
Opis:
This paper analyzes the requirements of the information transmission network of ship integrated condition monitoring system, and proposes a design scheme of ship condition monitoring system based on wireless ad hoc network. The wireless ad hoc network protocol was designed, its networking process was analyzed in detail, and the network transmission performance of the monitoring system was tested. The results proved the feasibility of the system. The above solution can be used for the transmission of ship state information that satisfies the requirements of wireless transmission, and has important theoretical and practical significance. The slot allocation algorithm has been receiving extensive attention as an important part of the TDMA system research. This paper analyzes the summarization and summarization of TDMA time slot assignment algorithms from several aspects such as slot synchronization, existing slot allocation algorithm, and slot assignment model, laying an important foundation for researchers to do further research. In the TDMA system, time is divided into non-overlapping time frames, and the time frames are divided into non-overlapping time slots. Each node in the network performs corresponding operations in each time slot.
Źródło:
Polish Maritime Research; 2018, S 2; 170-175
1233-2585
Pojawia się w:
Polish Maritime Research
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Gradient flow optimization for reducing blocking effects of transform coding
Autorzy:
Gao, F.
Li, X.
Wang, X.
Wee, W. G.
Powiązania:
https://bibliotekanauki.pl/articles/907251.pdf
Data publikacji:
2004
Wydawca:
Uniwersytet Zielonogórski. Oficyna Wydawnicza
Tematy:
przepływ gradientu
efekt blokowania
kodowanie transformatowe
gradient flow
blocking effects
optimization
transform coding
Opis:
This paper addresses the problem of reducing blocking effects in transform coding. A novel optimization approach using the gradient flow is proposed. Using some properties of the gradient flow on a manifold, an optimized filter design method for reducing the blocking effects is presented. Based on this method, an image reconstruction algorithm is derived. The algorithm maintains the fidelity of images while reducing the blocking effects. Experimental tests demonstrate that the presented algorithm is effective.
Źródło:
International Journal of Applied Mathematics and Computer Science; 2004, 14, 1; 105-111
1641-876X
2083-8492
Pojawia się w:
International Journal of Applied Mathematics and Computer Science
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Four stage hybrid constructed wetlands treating low-strength aquaculture wastewater with and without artificial aeration
Autorzy:
Zhang, S.-Y.
Li, G.
Li, X.
Tao, L.
Powiązania:
https://bibliotekanauki.pl/articles/207748.pdf
Data publikacji:
2015
Wydawca:
Politechnika Wrocławska. Oficyna Wydawnicza Politechniki Wrocławskiej
Tematy:
wastewater treatment
aquaculture
water pollution
wetlands
aquaculture wastewater
artificial aeration
oczyszczanie ścieków
akwakultura
zanieczyszczenie wody
mokradła
sztuczne napowietrzanie
tereny podmokłe
Opis:
Driven by the booming demands for healthy food, aquaculture industry has to deal with the problem of water pollution appropriately so as to achieve sustainable development. In this study, a combination of four stage CWs (three horizontal subsurface flows followed by one free water surface flow) was configured to treat low-strength aquaculture wastewater. For performance assessment, the wetlands were monitored over three years, during which artificial aeration was added to them. By the results, the organic matters and nutrients were mainly sequestered in the anterior subsurface flows, while the surface flow mainly contributed to DO improvement. These results probably implied no necessity of excessive subsurface flows connected in a staged manner. In addition, the artificial aeration improved the treatment performance on ammonium-N, TN and TP in the first-stage CW.
Źródło:
Environment Protection Engineering; 2015, 41, 3; 31-42
0324-8828
Pojawia się w:
Environment Protection Engineering
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The effect of high ship speed ratio on collision avoidance behavior of COLREGS
Autorzy:
Wang, X. H.
Li, L.
Chen, G.
Powiązania:
https://bibliotekanauki.pl/articles/116490.pdf
Data publikacji:
2019
Wydawca:
Uniwersytet Morski w Gdyni. Wydział Nawigacyjny
Tematy:
colregs
Personifying Intelligent Decision-making for Vessel Collision Avoidance (PIDVCA)
Vessel Collision Avoidance
collision avoidance
decision making
relative motion
geometricdiagram of relative motion
anti-collision
Opis:
The speed ratio is an important factor that must be considered when two vessels will course change to avoid collision. In the process of the research on Personifying Intelligent Decision-making for Vessel Collision Avoidance (short for PIDVCA), it is found that the effect of collision avoidance based on the existing “International Regulations for Prevention Collision at sea” (short for COLREGS) is greatly affected by the high speed ratio (k=Vt/V0≥1.5). Through the analysis on the geometric change law of two vessels’ relative motion in Open waters, the effects of the responsibility for the ship collision avoidance under the COLREGS and special case for high-speed ratio is discussed. According to the collision avoidance measures taken for two vessels encounter situation, some reasonable suggestions are put forward and the simulation experiments that based on ship's intelligent collision avoidance simulation platform are given to support the idea.
Źródło:
TransNav : International Journal on Marine Navigation and Safety of Sea Transportation; 2019, 13, 2; 319-323
2083-6473
2083-6481
Pojawia się w:
TransNav : International Journal on Marine Navigation and Safety of Sea Transportation
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
A Fast Method of Feature Extraction for Lowering Vehicle Pass-By Noise Based on Nonnegative Tucker3 Decomposition
Autorzy:
Wang, H.
Cheng, G.
Deng, G.
Li, X.
Li, H.
Huang, Y.
Powiązania:
https://bibliotekanauki.pl/articles/177883.pdf
Data publikacji:
2017
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
vehicle pass-by noise
NTD
feature extraction
sound pressure level
Opis:
Usually, the judgement of one type fault of vehicle pass-by noise is difficult for engineers, especially when some significant features are disturbed by other interference noise, such as the squealing noise is almost simultaneous with the whistle in the exhaust system. In order to cope with this problem, a new method, with the antinoise ability of the algorithm on the condition by which the features are entangled, is developed to extract clear features for the fault analysis. In the proposed method, the nonnegative Tucker3 decomposition (NTD) with fast updating algorithm, signed as NTD_FUP, can find out the natural frequency of the parts/components from the exhaust system. Not only does the NTD_FUP extract clear features from the confused noise, but also it is superior to the traditional methods in practice. Then, an aluminium-foil alloy material, which is used for the heat shield for its lower noise radiation, replaces the aluminium alloy alone. Extensive experiments show that the sound pressure level of the vehicle pass-by noise is reduced 0.9 dB(A) by the improved heat shield, which is also considered as a more lightweight design for the exhaust system of an automobile.
Źródło:
Archives of Acoustics; 2017, 42, 4; 619-629
0137-5075
Pojawia się w:
Archives of Acoustics
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The influence of landing mat composition on ankle injury risk during a gymnastic landing: a biomechanical quantification
Autorzy:
Xiao, X.
Xiao, W.
Li, X.
Wan, B.
Shan, G.
Powiązania:
https://bibliotekanauki.pl/articles/306922.pdf
Data publikacji:
2017
Wydawca:
Politechnika Wrocławska. Oficyna Wydawnicza Politechniki Wrocławskiej
Tematy:
symulacja komputerowa
właściwości mechaniczne
sztywność
tarcie
computer simulation
impact load
mechanical properties of floor mats
stiffness
dampness
friction
Opis:
Purpose: About 70% injury of gymnasts happened during landing – an interaction between gymnast and landing mat. The most injured joint is the ankle. The current study examined the effect of mechanical properties of landing mat on ankle loading with aims to identify means of decreasing the risk of ankle injury. Method: Gymnastic skill – salto backward stretched with 3/2 twist was captured by two high-speed camcorders and digitized by using SIMI-Motion software. A subject-specific, 14-segment rigid-body model and a mechanical landing-mat model were built using BRG.LifeMODTM. The landings were simulated with varied landing-mat mechanical properties (i.e., stiffness, dampness and friction coefficients). Result: Real landing performance could be accurately reproduced by the model. The simulations revealed that the ankle angle was relatively sensitive to stiffness and dampness of the landing mat, the ankle loading rate increased 26% when the stiffness was increased by 30%, and the changing of dampness had notable effect on horizontal ground reaction force and foot velocity. Further, the peak joint-reaction force and joint torque were more sensitive to friction than to stiffness and dampness of landing mat. Finally, ankle muscles would dissipate about twice energy (189%) when the friction was increased by 30%. Conclusion: Loads to ankles during landing would increase as the stiffness and dampness of the landing mat increase. Yet, increasing friction would cause a substantial rise of the ankle internal loads. As such, the friction should be a key factor influencing the risk of injury. Unfortunately, this key factor has rarely attracted attention in practice.
Źródło:
Acta of Bioengineering and Biomechanics; 2017, 19, 1; 105-113
1509-409X
2450-6303
Pojawia się w:
Acta of Bioengineering and Biomechanics
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
New application of D-π-A molecules as a sensor for recognition of Cu2+ in polluted water
Autorzy:
Niu, H.
Zhu, G.
Zhu, M.
Li, X.
Powiązania:
https://bibliotekanauki.pl/articles/207782.pdf
Data publikacji:
2017
Wydawca:
Politechnika Wrocławska. Oficyna Wydawnicza Politechniki Wrocławskiej
Tematy:
polluted water
nuclear magnetic resonance spectroscopy
Cu(II)
woda zanieczyszczona
spektroskopia magnetycznego rezonansu jądrowego
Opis:
Novel chromophore GZP1 with D-π-A structure was designed and prepared for its application as a sensor for the recognition of Cu2+ ions in polluted water. Reaction of aldimine condensation was used to synthesize chromophore GZP1. Its chemical structure of was characterized by mass spectrum, nuclear magnetic resonance spectroscopy and ultraviolet-visible absorption spectrum. Cu2+ ions were recognized via fluorescence spectra. Chromophore GZP1 showed specific detection ability of Cu2+. 14 other metal ions were used as reference. The detection limit was estimated to be 0.06 μmol/dm3 in aqueous solution, which was significantly lower than the typical concentration of blood Cu2+ in normal individuals and the limit of copper(II) in drinking water.
Źródło:
Environment Protection Engineering; 2017, 43, 4; 67-72
0324-8828
Pojawia się w:
Environment Protection Engineering
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Solution Growth of Well-Aligned ZnO Nanorods on Sapphire Substrate
Autorzy:
Jia, G.
Hao, B.
Lu, X.
Wang, X.
Li, Y.
Yao, J.
Powiązania:
https://bibliotekanauki.pl/articles/1399510.pdf
Data publikacji:
2013-07
Wydawca:
Polska Akademia Nauk. Instytut Fizyki PAN
Tematy:
68.37.Yz
78.40.Fy
68.55.J-
Opis:
Vertically well-aligned ZnO nanorods arrays were synthesized on sapphire substrates by chemical bath deposition. Those sapphire substrates were seeded to control the density and orientation of ZnO nanorods using sol-gel method. Well-aligned and uniformly distributed ZnO nanorods in a large scale were obtained with strongly (002) preferential orientation. The structural properties were characterized by X-ray diffraction spectrometer and morphological characteristics were analyzed by scanning electron microscopy, respectively. The ZnO nanorods are obvious hexangular wurtzite structure and preferentially oriented along the c-axis (002) and growth vertically to the substrates. The optical properties were further thoroughly studied. What is more, the influences of the strain between substrate and ZnO nanorods due to thickness of the ZnO seed-layer on the characteristics and optical properties of ZnO were also analyzed.
Źródło:
Acta Physica Polonica A; 2013, 124, 1; 74-77
0587-4246
1898-794X
Pojawia się w:
Acta Physica Polonica A
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Bacterial communities in PM2.5 and PM10 in broiler houses at different broiler growth stages in spring
Autorzy:
Zhang, J.
Li, Y.
Xu, E.
Jiang, L.
Tang, J.
Li, M.
Zhao, X.
Chen, G.
Zhu, H.
Yu, X.
Zhang, X.
Powiązania:
https://bibliotekanauki.pl/articles/2087465.pdf
Data publikacji:
2019
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
bacterial communities
broilers
high-throughput sequencing
particulate matter
Źródło:
Polish Journal of Veterinary Sciences; 2019, 3; 495-504
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Removal of quinoline from aqueous solutions by lignite, coking coal and anthracite. Adsorption isotherms and thermodynamics
Autorzy:
Xu, H.
Huagn, G.
Li, X.
Gao, L.
Wang, Y.
Powiązania:
https://bibliotekanauki.pl/articles/110016.pdf
Data publikacji:
2016
Wydawca:
Politechnika Wrocławska. Oficyna Wydawnicza Politechniki Wrocławskiej
Tematy:
quinoline adsorption
coking coal
adsorption isotherms
thermodynamics
Opis:
Based on the concept of circular economy, a novel method of industrial organic wastewater treatment by using adsorption on coal is introduced. Coal is used to adsorb organic pollutants from coking wastewaters. After adsorption, the coal would be used for its original purpose, its value is not reduced and the pollutant is thus recycled. Through systemic circulation of coking wastewater zero emissions can be achieved. Lignite, coking coal and anthracite were used as adsorbents in batch experiments. The quinoline removal efficiency of coal adsorption was investigated. The coking coal and anthracite exhibited properties well-suited for adsorption onto both adsorbents. The experimental data were fitted to Langmuir and Freundlich isotherms as well as Temkin, Redlich–Peterson (R-P) and Dubinin-Radushkevich (D-R) models. Both Freundlich Isotherm and D-R model provided reasonable models of the adsorption process. The thermodynamic parameters of quinoline adsorption on coking coal were calculated. The thermodynamic parameters indicated that the adsorption process is exothermic and is a physical adsorption. The △S° value indicated that the adsorption entropy decreased because the adsorbate molecule was under restrictions after it adsorption on the coal surface. The coal adsorption method for removing refractory organic pollutants is a great hope for achieving zero emission waste water for a coking plant.
Źródło:
Physicochemical Problems of Mineral Processing; 2016, 52, 1; 214-227
1643-1049
2084-4735
Pojawia się w:
Physicochemical Problems of Mineral Processing
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Adsorption behaviors of typical aromatic pollutants in biologically treated coking wastewater on powdered coal
Autorzy:
Sun, H.
Wang, Y.
Bian, Y.
He, X.
Li, G.
Powiązania:
https://bibliotekanauki.pl/articles/110777.pdf
Data publikacji:
2018
Wydawca:
Politechnika Wrocławska. Oficyna Wydawnicza Politechniki Wrocławskiej
Tematy:
aromatic pollutants
wastewater treatment
adsorption affinity
competitive adsorption
Opis:
The raw coal was utilized as adsorbent based on its remarkable adsorption ability of coal slime to organic compounds in the flotation process. This paper mainly investigates adsorption behaviors of benzpyrole (BZP), pyrrole (PR) and benzoic acid (BA) on powdered coal (PC). In the monocomponent solution, the removal efficiencies of BZP, PR and BA reached 85.23%, 55.02% and 24.84% at PC dosage of 20 g/dm3. Adsorption behaviors of three pollutants fitted perfectly to the pseudo-second order kinetics model and liquid film diffusion occupied the largest proportion in rate-limiting step according to the diffusion rates. Furthermore, the influences of pH on adsorption behaviors and competitive adsorption mechanism of three pollutants were also reported.
Źródło:
Physicochemical Problems of Mineral Processing; 2018, 54, 2; 496-504
1643-1049
2084-4735
Pojawia się w:
Physicochemical Problems of Mineral Processing
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Emerging of canine kobuvirus in dogsin China, 2015
Autorzy:
Liu, D.F.
Liu, X.
Li, Z.J.
F. Liu
Hu, X.L.
Li, Z.G.
Liu, C.G.
Ma, J.Z.
Powiązania:
https://bibliotekanauki.pl/articles/2087863.pdf
Data publikacji:
2017
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
canine kobuvirus (CaKoV)
dogs
phylogenetic
VP1
China
Źródło:
Polish Journal of Veterinary Sciences; 2017, 4; 707-711
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Unidirectional Torsion Properties of Single Silk Fibre
Właściwości monowłókien jedwabnych przy jednokierunkowym skręcaniu
Autorzy:
Jiang, S.
Cao, G.
Cai, G.
Xu, W.
Li, W.
Wang, X.
Powiązania:
https://bibliotekanauki.pl/articles/232187.pdf
Data publikacji:
2016
Wydawca:
Sieć Badawcza Łukasiewicz - Instytut Biopolimerów i Włókien Chemicznych
Tematy:
unidirectional torsion
silk fibre
fracture angle
breaking cycle
SEM
XRD
skręcanie jednokierunkowe
włókno jedwabne
badania
Opis:
The unidirectional torsion properties of silk fibre were investigated on a purpose-built single fibre torsion tester. The torsional fracture angle and the number of cycles of torsion at breaking were recorded, and the effect of the gauge length and pretension together with the torsion speed on the torsion properties of single silk fibre was investigated in detail. Scanning electron microscopy (SEM), X-ray diffraction (XRD) analysis and a tensile tester were used to understand the morphology, structure and tensile properties of silk fibre after torsion deformation. SME photos show that silk fibre exhibits a ribbon-like profile after torsion, and fracture tends to occur at both ends of the silk fibre, where a larger number of twists can be observed. The crystallinity calculated from XRD spectra of silk fibre increases from 26.11% to 34.10% after torsion. The breaking stress and strain decreases slightly with an increase in the gauge length. The breaking cycle increases linearly with an increase in the gauge length, while the actual torsional fracture angle decreases gradually at the same time. The torsional fracture angle together with the breaking cycle decreases gradually as the pretension increases. The fracture angle together with the breaking cycle increases with an increase in the torsion speed. Understanding the unidirectional torsion properties of single silk fibre will benefit its further application in specific areas where the fibre will be subject to frequent torsion and deformation.
Badania przeprowadzono za pomocą specjalnie zbudowanego urządzenia do badania skrętu włókien. Badano kąt przełomu oraz ilość cykli doprowadzających do zerwania. Sprawdzano również wpływ długości próbki oraz wstępnego naprężenia. Oceniono wpływ długości próbki wstępnego naprężenia, szybkości skrętu na właściwości wytrzymałościowe włókien jedwabnych. Stosowano SEM, XRD dla określenia morfologii i struktury włókien. Stwierdzono, że włókna jedwabne po badaniu wykazują formę podobną do taśmy. Na podstawie XRD stwierdzono, że krystaliczność wzrasta od ok. 26% do 34% po skręceniu. Naprężenie zrywające i odkształcenia zmniejszają się lekko przy wzroście długości próbki.
Źródło:
Fibres & Textiles in Eastern Europe; 2016, 3 (117); 26-30
1230-3666
2300-7354
Pojawia się w:
Fibres & Textiles in Eastern Europe
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Removal of quinoline from aqueous solutions by lignite, coking coal and anthracite. Adsorption kinetics
Autorzy:
Xu, H.
Wang, Y.
Huagn, G.
Fan, G.
Gao, L.
Li, X.
Powiązania:
https://bibliotekanauki.pl/articles/110664.pdf
Data publikacji:
2016
Wydawca:
Politechnika Wrocławska. Oficyna Wydawnicza Politechniki Wrocławskiej
Tematy:
quinoline adsorption
coking coal
kinetics
adsorption activation energy
coal adsorption
Opis:
Basing on the concept of circular economy, a novel method of industrial organic wastewater treatment by using adsorption on coal is introduced. Coal is used to adsorb organic pollutants from coking wastewaters. After adsorption, the coal would be used in its original purpose, as its value was not reduced and the pollutant was reused. Through the systemic circulation of coking wastewater zero emissions can be achieved. Lignite, coking coal and anthracite were used as adsorbents in batch experiments. The quinoline removal efficiency of coal adsorption was investigated. Both the coking coal and anthracite exhibited properties well-suited for quinoline adsorption removal. The experimental data were fitted to the pseudo-first- order and pseudo-second-order kinetic equations as well as intraparticle diffusion and Bangham models. An attempt was made to find the rate-limiting step involved in the adsorption processes. Both boundary-layer diffusion and intraparticle diffusion are likely involved in the rate-limiting mechanisms. Effect of pH on coal adsorptions by coking coal was investigated. The process of quinoline adsorption on coal was researched. The coal adsorption method for removing refractory organic pollutants is a great hope for achieving wastewater zero emission for coking plants.
Źródło:
Physicochemical Problems of Mineral Processing; 2016, 52, 1; 397-408
1643-1049
2084-4735
Pojawia się w:
Physicochemical Problems of Mineral Processing
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Electric Interaction between Two Identical Conducting Spheres in a Uniform Electric Field
Autorzy:
Gao, X.
Wang, Q.
Li, C.
Hu, L.
Sun, G.
Powiązania:
https://bibliotekanauki.pl/articles/1401870.pdf
Data publikacji:
2015-09
Wydawca:
Polska Akademia Nauk. Instytut Fizyki PAN
Tematy:
41.20.Cv
47.65.Gx
Opis:
An experimental apparatus is devised to measure the interaction between two spheres separated by a small gap in a uniform electric field. The results show that the interaction between two conducting spheres is near that between two dielectric spheres with high permittivity. Accordingly, the calculation can be simplified by mirror image method, for it is only available for conducting system. A method using multiple mirror images of point charges is put forward to analyze the induction of two identical conducting spheres in a uniform electric field. The key operation on how to add compensative charges is emphasized and given out in detail. The results from experiment and calculation are compared, and they agree with each other very well.
Źródło:
Acta Physica Polonica A; 2015, 128, 3; 289-293
0587-4246
1898-794X
Pojawia się w:
Acta Physica Polonica A
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Insensitive HMX (Octahydro-1,3,5,7-tetranitro-1,3,5,7-tetrazocine) Nanocrystals Fabricated by High-Yield, Low-Cost Mechanical Milling
Autorzy:
Wang, Y.
Jiang, W.
Song, X.
Deng, G.
Li, F.
Powiązania:
https://bibliotekanauki.pl/articles/358912.pdf
Data publikacji:
2013
Wydawca:
Sieć Badawcza Łukasiewicz - Instytut Przemysłu Organicznego
Tematy:
nanoexplosives
HMX
thermal decomposition
sensitivity
Opis:
A mechanical approach had been adopted for fabricating HMX nanoparticles. This fabrication method avoided the recrystallization process and was different from the traditional methods employed to prepare nanoexplosives. In particular, the high yield and low cost increased the possibility of its industrial application. Specifcally, HMX particles, that had a mean size of 0.27 μm, were prepared by mechanical milling; a signifcant proportion of nano-HMX (<100 nm) were present and these were observed by TEM and SEM images. The thermal decomposition of HMX samples before and after pulverization was investigated by TG/DSC analysis. The results indicated that there was no obvious difference between the thermographs of raw and pulverized HMX. The HMX samples were investigated by friction, impact, and shock sensitivity tests. High safety was confrmed since pulverized HMX was far more insensitive than raw HMX; indeed the shock sensitivity of pulverized HMX was about 60 percent lower than that of raw HMX.
Źródło:
Central European Journal of Energetic Materials; 2013, 10, 2; 277-287
1733-7178
Pojawia się w:
Central European Journal of Energetic Materials
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Investigations on the EPR Parameters of $KMgF_3$:$Cr^{+}$
Autorzy:
Kuang, M.
Wu, S.
Hu, X.
Li, G.
Zu, H.
Powiązania:
https://bibliotekanauki.pl/articles/1365348.pdf
Data publikacji:
2014-05
Wydawca:
Polska Akademia Nauk. Instytut Fizyki PAN
Tematy:
75.10.Dg
76.30.Fc
Opis:
The electron paramagnetic resonance parameters (i.e., g factor, hyperfine structure constant and superhyperfine parameters) of $KMgF_3$:$Cr^{+}$ are theoretically investigated from the perturbation formulae of these parameters for an octahedral $3 d^5$ cluster. As for the calculations of g factor and hyperfine structure constant, both the contributions from the crystal-field and charge transfer mechanisms are included based on the cluster approach. The metal to ligand charge transfer contribution to the g-shift Δg ( ≈ g-2.0023) is the same (negative) in sign and much larger in magnitude as compared to the crystal-field one. The conventional argument that the charge transfer contributions to zero-field splittings are negligible for $3 d^5$ ions in fluorides is no longer suitable for Δg analysis of $KMgF_3$:$Cr^{+}$ due to the dominant second-order charge transfer perturbation term. The charge transfer contribution to hyperfine structure constant exhibits the same sign and about 4% of the crystal-field one. The unpaired spin densities of the fluorine 2s, 2pσ and 2pπ orbitals are quantitatively acquired from the relationships with the relevant molecular orbital coefficients using the uniform model. The present treatments are superior to the previous calculations of directly fitting the experimental superhyperfine parameters.
Źródło:
Acta Physica Polonica A; 2014, 125, 5; 1224-1228
0587-4246
1898-794X
Pojawia się w:
Acta Physica Polonica A
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Analytical Descriptions of Dynamic Softening Mechanisms for Ti-13Nb-13Zr Biomedical Alloy in Single Phase and Two Phase Regions
Autorzy:
Quan, G.-Z.
Wang, X.
Li, Y.-L.
Zhang, L.
Powiązania:
https://bibliotekanauki.pl/articles/353917.pdf
Data publikacji:
2017
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
biomedical titanium alloy
flow stress
dynamic recovery
dynamic recrystallization
dynamic softening
Opis:
Dynamic softening behaviors of a promising biomedical Ti-13Nb-13Zr alloy under hot deformation conditions across dual phase α + β and single phase β regions were quantitatively characterized by establishing corresponding dynamic recovery (DRV) and dynamic recrystallization (DRX) kinetic models. A series of wide range hot compression tests on a Gleeble-3500 thermo-mechanical physical simulator were implemented under the strain rate range of 0.01-10 s−1 and the temperature range of 923-1173 K. The apparent differences of flow stress curves obtained in dual phase α + β and single phase β regions were analyzed in term of different dependence of flow stress to temperature and strain rate and different microstructural evolutions. Two typical softening mechanisms about DRV and DRX were identified through the variations of a series of stress-strain curves acquired from these compression tests. DRX is the dominant softening mechanism in dual phase α + β range, while DRV is the main softening mechanism in single phase β range. The DRV kinetic model for single phase β region and the DRX kinetic model for dual phase α + β region were established respectively. In addition, the microstructures of the compressed specimens were observed validating the softening mechanisms accordingly.
Źródło:
Archives of Metallurgy and Materials; 2017, 62, 4; 2029-2043
1733-3490
Pojawia się w:
Archives of Metallurgy and Materials
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Efficient learning variable impedance control for industrial robots
Autorzy:
Li, C.
Zhang, Z.
Xia, G.
Xie, X.
Zhu, Q.
Powiązania:
https://bibliotekanauki.pl/articles/200716.pdf
Data publikacji:
2019
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
variable impedance control
reinforcement learning
efficient
Gaussian process
industrial robots
impedancja
poprawa efektywności
wydajność
model Gaussa
roboty przemysłowe
Opis:
Compared with the robots, humans can learn to perform various contact tasks in unstructured environments by modulating arm impedance characteristics. In this article, we consider endowing this compliant ability to the industrial robots to effectively learn to perform repetitive force-sensitive tasks. Current learning impedance control methods usually suffer from inefficiency. This paper establishes an efficient variable impedance control method. To improve the learning efficiency, we employ the probabilistic Gaussian process model as the transition dynamics of the system for internal simulation, permitting long-term inference and planning in a Bayesian manner. Then, the optimal impedance regulation strategy is searched using a model-based reinforcement learning algorithm. The effectiveness and efficiency of the proposed method are verified through force control tasks using a 6-DoFs Reinovo industrial manipulator.
Źródło:
Bulletin of the Polish Academy of Sciences. Technical Sciences; 2019, 67, 2; 201-212
0239-7528
Pojawia się w:
Bulletin of the Polish Academy of Sciences. Technical Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
A multiplex PCR for simultaneous detection of classical swine fever virus, African swine fever virus, highly pathogenic porcine reproductive and respiratory syndrome virus, porcine reproductive and respiratory syndrome virus and pseudorabies in swines
Autorzy:
Hu, L.
Lin, X.Y.
Yang, Z.X.
Yao, X.P.
Li, G.L.
Peng, S.Z.
Wang, Y.
Powiązania:
https://bibliotekanauki.pl/articles/30249.pdf
Data publikacji:
2015
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
In this assay, we developed and evaluated a multiplex PCR (mPCR) for its ability in detecting multiple infections of swine simultaneously. Four pairs of primers were used to detect five viruses. Specific primers were designed for classical swine fever virus (CSFV), African swine fever virus (ASFV) and pseudorabies (PRV). A pair of primers was designed prudently for two different types of porcine reproductive and respiratory syndrome virus that respectively were porcine reproductive and respiratory syndrome virus (PRRSV), highly pathogenic porcine reproductive and respiratory syndrome virus (HP-PRRSV). The detection limits of the mPCR were 1.09×10⁴, 1.50×10³, 2.10×10³, 1.30×10³ and 8.97×10² copies/reaction for CSFV, ASFV, HP-PRRSV, PRRSV and PRV, respectively. A total of 49 clinical specimens were tested by the mPCR, and the result showed that co-infection by two or three viruses was 51%. In conclusion, the PCR is a useful tool for clinical diagnosis of not only single infections but also mixed infections in swines.
Źródło:
Polish Journal of Veterinary Sciences; 2015, 18, 4
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Defect Recovery in α-Fe e$\text{}^{-}$-irradiated at 300 K
Autorzy:
Dai, G. H.
Li, X. H.
Moser, P.
Moya, G.
Van Duysen, J. C.
Powiązania:
https://bibliotekanauki.pl/articles/1928989.pdf
Data publikacji:
1993-03
Wydawca:
Polska Akademia Nauk. Instytut Fizyki PAN
Tematy:
61.70.Bv
61.80.Fe
78.70.Bj
Opis:
Defect annealing recovery has been studied, by measuring positron lifetime spectra, in high-purity α-iron irradiated at 300 K with 3 MeV electrons to a fluency of 7 × 10$\text{}^{19}$ cm$\text{}^{-2}$. Vacancy clusters containing 6-10 single vacancies were observed immediately after irradiation during which they were possibly forming (the so-called "irradiation annealing"). With increasing temperature, the agglomerates continually grow in size at the expense of their concentration, giving rise to the formation of microvoids (> 15 vacancies). Also present were other types of defects, probably immobile vacancies trapped by impurity (e.g. carbon) atoms and dislocation/loops generated presumably from collapse of voids during the relatively high dose irradiation and/or the annealing. The immobile vacancies eventually became movable at around 350 K, supplying the growing clusters and thus leading to a stabilization in their concentration till around 500 K. Between 500 and 700 K, microvoids gradually evaporated, but the dislocation-associated defects were able to survive annealing at temperatures as high as 700 K. The void size and concentration and their evolution have been evaluated on the basis of both the to date theoretical and experimental studies. The temperature dependence was also, observed of positron trapping into vacancy agglomerates of various sizes.Za
Źródło:
Acta Physica Polonica A; 1993, 83, 3; 277-286
0587-4246
1898-794X
Pojawia się w:
Acta Physica Polonica A
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Effect of gas holdup on the efficiency of cyclonic-static microbubble flotation column for oily wastewater treatment
Autorzy:
Ning, K.
Huang, G.
Xu, H.
Wu, L.
Li, X.
Wang, Y.
Powiązania:
https://bibliotekanauki.pl/articles/208139.pdf
Data publikacji:
2018
Wydawca:
Politechnika Wrocławska. Oficyna Wydawnicza Politechniki Wrocławskiej
Tematy:
bubble columns
efficiency
flotation
wastewater treatment
oil water separation
kolumna flotacyjna
efektywność
flotacja
oczyszczanie ścieków
separacja wody
Opis:
A cyclonic-static microbubble flotation column of a novel construction was used in oil-water separation fields and has high efficiency for oil-water separation. The gas holdup is a key parameter for the evaluation of the performance of a flotation column. The gas holdup, closely related to the bubble size, bubble velocity and superficial gas velocity, is one of the most important parameters characterizing the hydrodynamics of a bubble column. The effect of gas holdup in a cyclonic-static microbubble flotation column was investigated. In addition, several operating parameters such as the circulating pressure, superficial gas velocity, and frother consumption were also investigated. The gas holdup was positively correlated to the superficial gas velocity. The gas holdup of clean water and oil wastewater increased along with the increase of the frother consumption. The separation mechanism of cyclonic-static microbubble flotation column was analyzed.
Źródło:
Environment Protection Engineering; 2018, 44, 2; 5-17
0324-8828
Pojawia się w:
Environment Protection Engineering
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Influence of the photosynthetic pathway on the hydrogen isotopic profile of glucose
Autorzy:
Zhang, B.
Billault, I.
Li, X.
Mabon, F.
Remaud, G.
Martin, M.
Powiązania:
https://bibliotekanauki.pl/articles/146974.pdf
Data publikacji:
2002
Wydawca:
Instytut Chemii i Techniki Jądrowej
Tematy:
deuterium
glucose
isotope ratio
plant metabolism
SNIF-NMR
Opis:
The SNIF-NMR method (site-specific natural isotope fractionation studied by Nuclear Magnetic Resonance) was used to examine the isotopic profile of glucoses derived from plants with different photosynthetic pathways. It is shown that the type of photosynthetic metabolism, either C3 (beet-root, orange, grape), C4 (maize, sugar-cane) or CAM (pineapple), exerts a strong influence on the deuterium distribution in the sugar molecules. The isotopic profile also depends, secondarily, on the physiological status of the precursor plant. Consequently, the isotopic fingerprint of glucose may be a rich source of information in mechanistic comparisons of metabolic pathways. Moreover, from an analytical point of view, it may provide complementary criteria with respect to the ethanol probe for origin inference of sugars.
Źródło:
Nukleonika; 2002, 47,suppl.1; 63-65
0029-5922
1508-5791
Pojawia się w:
Nukleonika
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Flotation of long flame coal pretreated by polyoxyethylene sorbitan monostearate
Autorzy:
Ni, C.
Xie, G.
Li, Z.
Bu, X.
Peng, Y.
Sha, J.
Powiązania:
https://bibliotekanauki.pl/articles/110299.pdf
Data publikacji:
2016
Wydawca:
Politechnika Wrocławska. Oficyna Wydawnicza Politechniki Wrocławskiej
Tematy:
flotation
hydrophobicity
low rank coal
Tween 60
pretreatment
Opis:
In this investigation, polyoxyethylene sorbitan monostearate (Tween 60) was used to improve flotation of fine long flame coal. The flotation recovery of long flame coal could be increased when long flame coal was either pretreated or conditioned with Tween 60 in a flotation cell for a period before the addition of collector. Fourier Transform Infrared (FTIR) technique was used to indicate surface properties of long flame coal. The results of FTIR show that there are many oxygen functional groups on the sur-face. Contact angle measurements were used to indicate changes in hydrophobicity of coal surface before and after Tween 60 and/or diesel pretreatments. The results of contact angle measurements show that hydrophobicity of coal can be increased by Tween 60. Tween 60 can also enhance adsorption of diesel on the coal surface, and hence floatability of long flame coal can be further improved. Tween 60 primarily enhances the flotation recovery of low density coal fractions (<1.5 and 1.5-1.8 kg/dm3). However, the increase in flotatation recovery is less significant with an excessive addition of Tween 60.
Źródło:
Physicochemical Problems of Mineral Processing; 2016, 52, 1; 317-327
1643-1049
2084-4735
Pojawia się w:
Physicochemical Problems of Mineral Processing
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Dft investigation of a high energy density polynitro compound, 2,2’-Bis(trinitromethyl)-5,5’-azo-1,2,3,4- tetrazole
Autorzy:
Lin, H.
Zhu, S. G.
Chen, P. Y.
Li, K.
Li, H. Z.
Peng, X. H.
Powiązania:
https://bibliotekanauki.pl/articles/358030.pdf
Data publikacji:
2013
Wydawca:
Sieć Badawcza Łukasiewicz - Instytut Przemysłu Organicznego
Tematy:
polynitro
electronic structure
thermodynamic properties
crystal structure
detonation performance
stability
Opis:
A novel polynitro compound, 2,2’-bis(trinitromethyl)-5,5’-azo- 1,2,3,4-tetrazole, was designed and investigated at the DFT-B3LYP/6-31G(d) level. Its properties, such as electronic structure, IR spectrum, heat of formation, thermodynamic properties and crystal structure, were predicted. This compound is most likely to crystallize in the P21 space group, and the corresponding cell parameters are Z = 2, a = 5.46 Å, b = 9.72 Å, c = 14.05 Å, α = 90°, β = 90°, γ = 90°. In addition, the detonation velocity and pressure were also estimated by using the empirical Kamlet-Jacobs equations, and were predicted to be 8.28 km/s and 31.61 GPa respectively. The oxygen balance of this compound is +13.79%, which indicates that it could serve as an oxidizer. Bond dissociation energy calculations show that the C(13)-N(21)O2 and C(14)-N(30)O2 bonds are the locations of thermal decomposition and that this compounds meets the thermal stability requirements as an exploitable explosive. Keywords: polynitro, electronic structure, thermodynamic properties, crystal structure, detonation performance, stability.
Źródło:
Central European Journal of Energetic Materials; 2013, 10, 3; 325-338
1733-7178
Pojawia się w:
Central European Journal of Energetic Materials
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Isolation and genetic analysis of a variant porcine epidemic diarrhea virus in China
Autorzy:
Li, R.-f.
Tian, X.-g.
Liu, Y.
Xu, J.
Liu, D.-y.
Powiązania:
https://bibliotekanauki.pl/articles/30492.pdf
Data publikacji:
2016
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
Porcine epidemic diarrhea virus (PEDV) is having a severe effect on the pig breeding industry in central China. The mucosa and the content of the small intestine from newborn pre-weaned piglets with diarrhea were tested for the presence of PEDV by molecular and morphologic methods, and found to be positive. Negative-staining electron microscopy (EM) revealed the presence of coronavirus- like particles in the samples. The result of molecular detection by nested RT-PCR based on the amplification of the M gene was positive. Using a novel alternative method we successfully propagated the PEDV strain (CH/QX-2) in Vero cells, confirmed by ultrathin sections of the cells and Immunofluorescence assay (IFA). Phylogenetic analysis based on the partial S gene showed that the CH/QX-2 isolate was genetically closer to strains more commonly found in China, but differed genetically from two domestic strains (CH/S, 1986 and LZC, 2007), Korean strains (DR13, 2007), and the vaccine strain (CV777 vs) currently being used in China. CH/QX-2 formed a unique clade in the derived phylogenetic tree indicating that the CH/QX-2 strain currently circulating in central China is a new variant of PEDV. This study extends current knowledge on the diversity and epidemiology of PEDV.
Źródło:
Polish Journal of Veterinary Sciences; 2016, 19, 1
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Influence of Polytetrafluorethylene on the Mechanical and Safety Properties of a Composite Modified Double Base Propellant
Autorzy:
Sun, S.
Zhang, T.
Zhao, B.
Zhang, G.
Li, X.
Luo, Y.
Powiązania:
https://bibliotekanauki.pl/articles/358296.pdf
Data publikacji:
2018
Wydawca:
Sieć Badawcza Łukasiewicz - Instytut Przemysłu Organicznego
Tematy:
PTFE fibres
CMDB propellant
mechanical properties
mechanical sensitivity
combustion
Opis:
A novel Composite Modified Double Base (CMDB) propellant, formed by mechanically mixing aluminium/polytetrafluorethylene (Al/PTFE) powders, was prepared through a rolling process. A variety of tests, such as tensile properties, particle size analysis etc., were carried out to study the influence of PTFE on the CMDB propellant properties. The PTFE deformed from particles to fibres under a uniform shear force, forming a fibre network which greatly improved the propellant’s mechanical properties. Compared to that of the CMDB propellant without PTFE, the elongation of the propellant containing 6% PTFE was increased by 26 times, and moreover, the impact strength was enhanced by 326% at −40 °C. Significantly, the propellant friction and impact sensitivities were reduced by 75.8% and 35.6%, respectively. In addition, the presence of PTFE in the propellant resulted in fluorination of the Al. The gaseous combustion product AlF3 reduced the propellant combustion agglomeration. Consequently, PTFE significantly promoted the propellant’s mechanical performance, decreased the shock (friction, impact) sensitivity and reduced combustion agglomeration.
Źródło:
Central European Journal of Energetic Materials; 2018, 15, 3; 468-484
1733-7178
Pojawia się w:
Central European Journal of Energetic Materials
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Effect of Ageratina adenophora on hepatic and pulmonic pathological lesions in horses
Autorzy:
Gu, X.L.
Dai, F.Y.
Xiao, X.
Li, G.Z.
Zhang, L.M.
Qu, W.J.
Powiązania:
https://bibliotekanauki.pl/articles/2087129.pdf
Data publikacji:
2021
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
Ageratina adenophora
liver
lung
horse
toxicity
Źródło:
Polish Journal of Veterinary Sciences; 2021, 24, 4; 611-613
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Research on the influence of excavation and loading on Z-Direction displacement in surrounding soil mass
Badania wpływu prac wydobywczych i składowania na wielkość przemieszczenia gruntu w kierunku z w otaczającym górotworze
Autorzy:
Li, X.
Zhou, W.
Han, L.
Xi, W. J.
Crusoe Jr., G. E.
Powiązania:
https://bibliotekanauki.pl/articles/220213.pdf
Data publikacji:
2014
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
wydobycie
składowanie
wytrzymałość gruntu w trakcie składowania
naprężenia
przemieszczenia
kołowy przebieg przemieszczenia
excavation
loading
unloading strength
stress
region displacement
displacement circle
Opis:
To probe into the pattern in which the excavation and loading process have on such factors as stress and displacement in neighboring regions of deep open pits, a mechanical unloading model in coal mining process and another model for the loading process are set up respectively. Besides, FLAC3D software is used to simulate dynamic excavating and loading process in open pits and record such data as the unbalanced stress, unloading strength and displacement fluctuations, which further serve as basis for studying the functional relationship about different mining heights and scope of influence using fitting method. The research results indicate that the unloading strength enhances with increasing mining depth in a linear fashion. In addition, a noticeable displacement circle takes shape around the stope, which would also extends with growing mining depth. As to waste loading, it brings about large-scale surface subsidence in neighboring regions, which follows a logarithm function convergence pattern with the distance away from the dump border. Under combined effects of excavation and loading, the value of the soil mass displacement would increase with growing mining depth and loading height. Specifically, the soil displacement at a distance of 100 m away from the stope border (around 200 m away from the outer dump border) is abnormally significant and it further develops at a rate of 0.0228 mm/h.
W celu zbadania zależności pomiędzy procesami wydobycia i składowania urobku a takimi czynnikami jak naprężenia i przemieszczenia w sąsiadujących partiach głębokich wyrobisk odkrywkowych, wykorzystano model mechaniczny procesu urabiania węgla oraz model procesu jego składowania. Wykorzystano oprogramowanie FLAC 3D do symulacji procesów wydobycia i składowania w wyrobiskach odkrywkowych, zapisano odpowiednie dane: niezrównoważone naprężenia, wytrzymałość w trakcie odciążania oraz wahania przemieszczeń gruntu, które wykorzystane zostały następnie jako podstawa do badania funkcjonalnych zależności pomiędzy wysokością poziomu wydobycia a skalą oddziaływania, przy użyciu odpowiednich metod dopasowania. Wyniki badań wskazują, że wytrzymałość w trakcie odciążania wzrasta liniowo z głębokością. Ponadto, zarejestrowano wyraźny kołowy przebieg przemieszczenia wokół zbocza, który także rośnie z głębokością. W przypadku obciążenia wskutek urobku odpadowego, zanotowano obniżenia terenu o dużym zasięgu o przebiegu konwergencji opisanym funkcją logarytmiczną odległości od miejsca składowania urobku odpadowego. W przypadku wystąpienia połączonych efektów wydobycia i składowania, wielkość przemieszczeń mas gruntu wzrastać będzie wraz z głębokością prowadzenia prac i wysokością składowania. Przemieszczenie gruntu w odległości 100 m od brzegu wyrobiska (ok. 200 od zewnętrznej granicy składowiska) okazuje się niepokojąco duże i postępujące w tempie 0.0228 mm/h).
Źródło:
Archives of Mining Sciences; 2014, 59, 4; 959-970
0860-7001
Pojawia się w:
Archives of Mining Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The Microstructural Evolution in HMX Based Plastic-bonded Explosive During Heating and Cooling Process: an in situ Small-angle Scattering Study
Autorzy:
Yan, G.
Tian, Q.
Liu, J.
Fan, Z.
Sun, G.
Zhang, C.
Wang, Y.
Chen, B.
Gong, J.
Zhou, X.
Yang, Z.
Nie, F.
Li, J.
Li, X.
Powiązania:
https://bibliotekanauki.pl/articles/358618.pdf
Data publikacji:
2016
Wydawca:
Sieć Badawcza Łukasiewicz - Instytut Przemysłu Organicznego
Tematy:
SANS
SAXS
HMX-PBX
thermal damages
phase transition
Opis:
The thermal damage in octahydro-1,3,5,7-tetranitro-1,3,5,7-tetrazocine (HMX) based plastic-bonded explosive (PBX) was investigated using in situ small-angle neutron and X-ray scattering techniques. The microstructural evolution was quantitatively characterized by the model fitting parameters of total interfacial surface area (Sv) and void volume distribution. The Sv of HMX-PBX decreased markedly above 100 °C, indicating the movement of binder into the voids. After subsequent cooling to room temperature, the scattering intensity increased significantly with increasing storage time, and a new population of voids with average diameter of 20 nm was observed, accompanied by the gradual phase transition of HMX from δ- to β-phase. The experimental results implied that serious damage within the HMX-PBX was developed during storage after heating.
Źródło:
Central European Journal of Energetic Materials; 2016, 13, 4; 916-926
1733-7178
Pojawia się w:
Central European Journal of Energetic Materials
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Impact of different sources of donor cells upon the nuclear transfer efficiency in Chinese indigenous Meishan pig
Autorzy:
Hua, Z.
Xu, G.
Liu, X.
Bi, Y.
Xiao, H.
Hua, W.
Li, L.
Zhang, L.
Ren, H.
Zheng, X.
Powiązania:
https://bibliotekanauki.pl/articles/31224.pdf
Data publikacji:
2016
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
Somatic cell nuclear transfer (SCNT) is currently the most efficient and precise method to generate genetically tailored pig models for both agricultural and biomedical research. However, its efficiency is crucially dependent on the source of nuclear donor cells. In this study, we compared the cloning efficiency by using three lines of donor cells that are derived from fetal, newborn and adult fibroblasts of Chinese indigenous Meishan pig. We showed that cleavage rate and blastocyst formation rate of the reconstructed embryos were not significantly different between the fetal (80.7% and 15.6%) and newborn ear skin (77.5% and 12.3%) fibroblast groups (p>0.05), but in both groups these indices were significantly higher than that found in the adult ear skin (70.5% and 8.8%; p<0.05). Reconstructed embryos derived from fetal, newborn, and adult ear skin fibroblasts were transferred to four surrogates, respectively. For the fetal, newborn, and adult ear skin fibroblasts, the number of pregnancies were two (50.0%), two (50.0%), and one (25.0%), respectively, and the number of deliveries were two (50.0%), one (25.0%), and zero (0.0%), respectively. Seven and two cloned piglets were obtained from the fetal and newborn ear skin fibroblasts respectively, while no piglets were obtained from the adult ear skin fibroblasts. Two cloned piglets from the newborn ear skin fibroblasts died shortly after birth because of neonatal asphyxia caused by dystocia. The birth weights of the piglets derived from the fetal and newborn ear skin fibroblasts were 1230.5 and 1310.0g, respectively, which were statistically insignificant (p>0.05), but both were significantly higher than that of the control groups (p<0.05). Microsatellite analyses demonstrated that the genotypes of all cloned piglets were identical to their donor cells. Therefore, cloned pigs were successfully produced using two sources of donor cells isolated from the fetal and newborn ear skin fibroblasts of Meishan piglet, and indicating a better cloning efficiency than that obtained from adult fibroblasts. We concluded that the nuclear donor cell lines have significant impact on the developmental competence of cloned embryos as well as on the cloning efficiency of Meishan pig.
Źródło:
Polish Journal of Veterinary Sciences; 2016, 19, 1
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Easy Vortex Motion in an Artificial Channel of $YBa_2Cu_3O_{7-δ}$ Superconducting Films
Autorzy:
Jukna, A.
Barboy, I.
Jung, G.
Abrutis, A.
Li, X.
Wang, D.
Sobolewski, R.
Powiązania:
https://bibliotekanauki.pl/articles/1813381.pdf
Data publikacji:
2008-03
Wydawca:
Polska Akademia Nauk. Instytut Fizyki PAN
Tematy:
74.25.Sv
74.25.Qt
74.78.Bz
Opis:
Quasi-Josephson effect produced by a coherent vortex motion in the horizontal part of the laser-performedΠ-shaped channel of a $YBa_2Cu_3O_{7-δ}$ superconducting bridge was investigated by means of electric transport measurements. We observed that in our structures, in a limited range of temperatures and bias currents, the vortices were confined in the channel only and moved coherently with the velocity of $3×10^4$ m/s. The corresponding current-voltage characteristics of the bridge exhibited Josephson-like voltage steps with the amplitude dependent on temperature, but independent of the bias current.
Źródło:
Acta Physica Polonica A; 2008, 113, 3; 959-962
0587-4246
1898-794X
Pojawia się w:
Acta Physica Polonica A
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Oxygen-containing Tetrazole Salts of 3-Hydrazino-4-amino-1,2,4-triazole (HATr): Nitrogen-rich Ionic Materials with High Thermal Stability
Autorzy:
Wu, J.-T.
Zhang, J.-G.
Yin, X.
Li, T.
Wu, L.
Zhang, Z.-B.
Powiązania:
https://bibliotekanauki.pl/articles/358927.pdf
Data publikacji:
2017
Wydawca:
Sieć Badawcza Łukasiewicz - Instytut Przemysłu Organicznego
Tematy:
energetic salts
thermal stability
oxygen-containing tetrazoles
3-hydrazino-4-amino-1,2,4-triazole
Opis:
Energetic salts of doubly-protonated 3-hydrazino-4-amino-1,2,4-triazole (2-4), and mono-protonated 3-hydrazino-4-amino-1,2,4-triazole (5-7), have been prepared in high yields from the corresponding oxygen-containing tetrazoles by (i) reaction of free acids and neutral 3-hydrazino-4-amino-1,2,4-triazole, or (ii) metathesis reactions of 3-hydrazinium-4-amino-1H-1,2,4-triazolium di-chloride with the silver salts of the corresponding oxygen-containing tetrazoles. All of these energetic salts were fully characterized by single-crystal X-ray diffraction, FT-IR and DSC measurements. All of the structures are dominated by extensive hydrogen bonds due to amino groups, hydrazino groups and oxygen atoms in the molecules. These salts exhibit good thermal stability, especially the salt 3-hydrazinium-4-amino-1H-1,2,4-triazolium 1H,1’H-5,5’-bitetrazole-1,1’-diolate (4), which exhibits a measured onset of decomposition temperature of 237 °C. Quantum chemical calculations, carried out using the Gaussian 03 suite of programs and based on the experimental densities were used to calculate the detonation pressures and velocities of all of the salts.
Źródło:
Central European Journal of Energetic Materials; 2017, 14, 1; 217-232
1733-7178
Pojawia się w:
Central European Journal of Energetic Materials
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Thermally induced damage in hexanitrohexaazaisowurtzitane
Autorzy:
Tian, Q.
Yan, G.
Sun, G.
Huang, C.
Xie, L.
Chen, B.
Huang, M.
Li, H.
Liu, X.
Wang, J.
Powiązania:
https://bibliotekanauki.pl/articles/358735.pdf
Data publikacji:
2013
Wydawca:
Sieć Badawcza Łukasiewicz - Instytut Przemysłu Organicznego
Tematy:
CL-20
SAXS
AFM
thermal effects
voids
Opis:
The evolution of the microstructure of hexanitrohexaazaisowurtzitane (CL-20) after a thermal stimulus plays a key role in the performance of CL-20. In the current work, microstructural variations of CL-20 caused by thermal treatment were investigated by X-ray diffraction, in situ variable-temperature optical microscopy, atom force microscopy, and small-angle X-ray scattering. A wave-like process, an abrupt volume expansion, movement, and fragmentation of CL-20 particles during phase transition were observed. After the phase transition (160 °C) the CL-20 sample acquired a very rough surface with numerous dimple depressions, and during the thermal decomposition stage (200 °C) a large number of voids were produced in both the bulk and the surface of the CL-20.
Źródło:
Central European Journal of Energetic Materials; 2013, 10, 3; 359-369
1733-7178
Pojawia się w:
Central European Journal of Energetic Materials
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Expression and immunological evaluation of elongation factor Tu of Streptococcus suis serotype 2
Autorzy:
Xia, X.J.
Wang, L.
Cheng, L.K.
Shen, Z.Q.
Li, S.G.
Wang, J.L.
Powiązania:
https://bibliotekanauki.pl/articles/31256.pdf
Data publikacji:
2017
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
Streptococcus suis serotype 2 (SS2) is considered as a major pathogen that causes sepsis and meningitis in piglets and humans, but knowledge of its antigenic proteins remains limited so far. The surface-related proteins of pathogens often play significant roles in bacterium-host interactions and infection. Here, we obtained the elongation factor Tu (EF-Tu) gene of Streptococcus suis and constructed the recombinant expression plasmid successfully. The target recombinant plasmid was then expressed in Escherichia coli and the immuno-protection of the recombinant protein was subsequently evaluated as well. The EF-Tu gene of Streptococcus suis is 1197 bp in length, encodes 398 amino acids. The target recombinant EF-Tu (rEF-Tu) protein can recognize the antiserum of Streptococcus suis and can provoke obvious humoral immune responses in rabbits and conferred protection to rabbits against Streptococcus suis ear-vein challenge, implying that the EF-Tu may be used as an attractive candidate antigen for a component of subunit vaccine.
Źródło:
Polish Journal of Veterinary Sciences; 2017, 20, 2
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Optimal expression and purification of sapelovirus A structural protein VP1, and its immunogenicity in mice
Autorzy:
Zhao, T.T.
Cui, L.
Chen, L.
Li, J.J.
Liang, Q.L.
Wu, P.J.
Yu, X.Q.
Zhang, Z.H.
Hua, X.G.
Powiązania:
https://bibliotekanauki.pl/articles/2087686.pdf
Data publikacji:
2018
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
sapelovirus A
prokaryotic expression
purification
Źródło:
Polish Journal of Veterinary Sciences; 2018, 21, 3; 573-579
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The distribution of different virulence grass carp reovirus strains in some neglected tissues
Autorzy:
Liang, H.R.
Fu, X.Z.
Li, N.Q.
Liu, L.H.
Lin, Q.
Li, Y.G.
Peng, Y.A.
Huang, Z.B.
Wu, S.Q.
Powiązania:
https://bibliotekanauki.pl/articles/30668.pdf
Data publikacji:
2016
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Źródło:
Polish Journal of Veterinary Sciences; 2016, 19, 4
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Identification of a linear epitope in the capsid protein of goose astrovirus with monoclonal antibody
Autorzy:
Dai, G.
Huang, X.
Liu, Q.
Li, Y.
Zhang, L.
Han, K.
Yang, J.
Liu, Y.
Xue, F.
Zhao, D.
Powiązania:
https://bibliotekanauki.pl/articles/16647377.pdf
Data publikacji:
2022
Wydawca:
Polska Akademia Nauk. Czasopisma i Monografie PAN
Tematy:
epitope
goose astrovirus
capsid protein
monoclonal antibody
Opis:
Goose astrovirus (GoAstV) is a novel avastrovirus that typically causes gosling gout and results in 2 to 20% mortality. GoAstV capsid protein is the sole structural protein, which is responsible for viral attachment, assembly, maturation as well as eliciting host antibodies. However, the epitopes within capsid protein have not been well studied. In this study, a monoclonal antibody, named 1D7, was generated against GoAstV capsid protein by hybridoma technology. Western blot results showed that this MAb could react with recombinant capsid protein expressed in E. coli. Also, it recognized the precursor of capsid protein, VP90 and VP70, in GoAstV-infected cells. Besides, excellent specificity of MAb 1D7 was further demonstrated in indirect immunofluorescence assay and immunohistochemical analysis. Epitope mapping results revealed that MAb 1D7 recognized the epitope 33QKVY 36 within Cap protein. Sequence alignment indicated that 33QKVY 36 is a conserved epitope among the isolates of goose astrovirus type 2 (GoAstV-2), suggesting the potential for its use in GoAstV-2 specific diagnostic assay. These findings may provide some insight into a function of the GoAstV capsid protein and further contribute to the development of diagnostic methods for GoAstV infection.
Źródło:
Polish Journal of Veterinary Sciences; 2022, 25, 4; 579-587
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Rapid screening of monoclonal antibodies against porcine circovirus type 2 using colloidal gold-based paper test
Autorzy:
Jin, Q.Y.
Feng, L.L.
Wang, Y.B.
Li, P.
Yang, J.F.
Teng, M.
Chai, S.J.
Xing, G.X.
Zhang, G.P.
Powiązania:
https://bibliotekanauki.pl/articles/16539073.pdf
Data publikacji:
2022
Wydawca:
Polska Akademia Nauk. Czasopisma i Monografie PAN
Tematy:
porcine circovirus type 2 (PCV2)
screening of MAbs
paper test
Opis:
A proof of concept for using paper test as a suitable method in the production of monoclonal antibodies (MAbs) is reported. The paper test which detects antibodies against porcine circovirus type 2 (PCV2) using colloidal gold-labelled capsid protein as the antigen probe was applied exclusively in the screening of anti-PCV2 MAbs. It allowed the detection of 118 single cell clones within 30 min using naked eyes. MAbs with specific binding to authentic epitopes on the virus were selected using a blocking strategy in which the antibody was pre-incubated with PCV2 viral sample before applying to the test paper. Five hybridomas secreting MAbs against the capsid protein were obtained, with only three of them capable of binding to PCV2. The results were validated and confirmed using enzyme-linked immunosorbent assay and immunofluorescence assay. The paper test is simple, rapid, and independent on professional technicians and proves to be an excellent approach for the screening of MAbs against specific targets.
Źródło:
Polish Journal of Veterinary Sciences; 2022, 25, 1; 27-34
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
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