Informacja

Drogi użytkowniku, aplikacja do prawidłowego działania wymaga obsługi JavaScript. Proszę włącz obsługę JavaScript w Twojej przeglądarce.

English (EN)·Polski (PL)·Yкраїнський (UK)
Przejdź do strony domowej biblioteki Centralna Biblioteka Wojskowa Link otwiera się w nowym oknie Logo biblioteki Prolib Integro - strona głównaCentralna Biblioteka Wojskowa

Wyszukujesz frazę "gene" wg kryterium: Wszystkie pola

  Lista filtrów

Dostawca treści

Podbaza

Wyrównaj
Wyświetlanie 1-50 z 1094
Tytuł:
Conceptualization of the Gene in Richard Dawkins’ The Selfish Gene
Autorzy:
Drogosz, Anna
Powiązania:
https://bibliotekanauki.pl/articles/2016189.pdf
Data publikacji:
2017
Wydawca:
Komisja Nauk Filologicznych Polskiej Akademii Nauk, Oddział we Wrocławiu
Tematy:
personification
blending
selfish gene
metaphor in science
Opis:
This paper investigates the conceptualization of the gene in the book The Selfish Gene by Richard Dawkins from the point of view of the Conceptual Metaphor Theory and the Conceptual Blending Theory. It is argued that there are two senses of the gene: gene1 is a physically existing section of the DNA, gene2 is information about protein synthesis. It is the second sense of the concept of the gene that undergoes metaphorization. The analysis reveals that Dawkins’ gene is a conceptual blend which becomes extensively elaborated. Through elaboration the gene becomes personified on the one hand and deified on the other. The study shows the richness of Dawkins’ personification: the gene is conceptualized as building and controlling organisms, cooperating and competing with other genes, even showing personality traits. Deification of the gene is focused on highlighting its stipulated immortality and power to create organisms, humans included. The gene blend is at the same time used as an input space in the integration network producing the meme blend. It is also established that the personification of the gene plays the theory-constitutive role in the sense of Boyd (1993).
Źródło:
Academic Journal of Modern Philology; 2017, 6; 75-84
2299-7164
2353-3218
Pojawia się w:
Academic Journal of Modern Philology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Analysis of single gene multitrait effects in livestock by the use of Gibbs sampling
Autorzy:
Dobek, A
Molinski, K.
Szydlowski, M.
Szwaczkowski, T.
Powiązania:
https://bibliotekanauki.pl/articles/2042024.pdf
Data publikacji:
2000
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
gene
gene frequency
major gene
livestock
Gibbs sampling
Źródło:
Journal of Applied Genetics; 2000, 41, 4; 275-283
1234-1983
Pojawia się w:
Journal of Applied Genetics
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Antiangiogenic gene therapy in inhibition of metastasis.
Autorzy:
Szala, Stanisław
Szary, Jarosław
Cichoń, Tomasz
Sochanik, Aleksander
Powiązania:
https://bibliotekanauki.pl/articles/1043756.pdf
Data publikacji:
2002
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
antiangiogenic gene therapy
encapsulation
inducible gene expression
metastasis
Opis:
This short review attempts to demonstrate the usefulness of antiangiogenic gene therapy in achieving inhibition of growth in experimentally-induced metastases. Certain normal tissues (for example skeletal muscle) may be used in vivo, after genetic modification, as a "bioreactor", able to produce and secrete into the bloodstream proteins known to exert antiangiogenic effects. By inhibiting neoangiogenesis these proteins would thus prevent the development of metastases. The review discusses also the perspectives of antimetastatic therapy based on certain types of allogenic cells (for example myoblasts and fibroblasts) that had been genetically modified and then microencapsulated. The strategy of encapsulation is aimed at protecting the modified cells secreting antiangiogenic factors from being eliminated by the immune system. Secretion of antiangiogenic proteins by these microencapsulated cells can be controlled with inducible promoters. Antiangiogenic genes remaining under the transcriptional control of such promoters may be switched on and off using antibiotics, such as tetracycline derivatives, or steroid hormones.
Źródło:
Acta Biochimica Polonica; 2002, 49, 2; 313-321
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Data mining methods for gene selection on the basis of gene expression arrays
Autorzy:
Muszyński, M.
Osowski, S.
Powiązania:
https://bibliotekanauki.pl/articles/329803.pdf
Data publikacji:
2014
Wydawca:
Uniwersytet Zielonogórski. Oficyna Wydawnicza
Tematy:
gene expression array
gene ranking
feature selection
clusterization measures
fusion
SVM classification
ekspresja genów
selekcja cech
klasyfikacja SVM
Opis:
The paper presents data mining methods applied to gene selection for recognition of a particular type of prostate cancer on the basis of gene expression arrays. Several chosen methods of gene selection, including the Fisher method, correlation of gene with a class, application of the support vector machine and statistical hypotheses, are compared on the basis of clustering measures. The results of applying these individual selection methods are combined together to identify the most often selected genes forming the required pattern, best associated with the cancerous cases. This resulting pattern of selected gene lists is treated as the input data to the classifier, performing the task of the final recognition of the patterns. The numerical results of the recognition of prostate cancer from normal (reference) cases using the selected genes and the support vector machine confirm the good performance of the proposed gene selection approach.
Źródło:
International Journal of Applied Mathematics and Computer Science; 2014, 24, 3; 657-668
1641-876X
2083-8492
Pojawia się w:
International Journal of Applied Mathematics and Computer Science
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Ensemble of data mining methods for gene ranking
Autorzy:
Wiliński, A.
Osowski, S.
Powiązania:
https://bibliotekanauki.pl/articles/201570.pdf
Data publikacji:
2012
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
gene expression array
feature selection
gene ranking methods
classification
SVM
Opis:
The paper presents the ensemble of data mining methods for discovering the most important genes and gene sequences generated by the gene expression arrays, responsible for the recognition of a particular type of cancer. The analyzed methods include the correlation of the feature with a class, application of the statistical hypotheses, the Fisher measure of discrimination and application of the linear Support Vector Machine for characterization of the discrimination ability of the features. In the first step of ranking we apply each method individually, choosing the genes most often selected in the cross validation of the available data set. In the next step we combine the results of different selection methods together and once again choose the genes most frequently appearing in the selected sets. On the basis of this we form the final ranking of the genes. The most important genes form the input information delivered to the Support Vector Machine (SVM) classifier, responsible for the final recognition of tumor from non-tumor data. Different forms of checking the correctness of the proposed ranking procedure have been applied. The first one is relied on mapping the distribution of selected genes on the two-coordinate system formed by two most important principal components of the PCA transformation and applying the cluster quality measures. The other one depicts the results in the graphical form by presenting the gene expressions in the form of pixel intensity for the available data. The final confirmation of the quality of the proposed ranking method are the classification results of recognition of the cancer cases from the non-cancer (normal) ones, performed using the Gaussian kernel SVM. The results of selection of the most significant genes used by the SVM for recognition of the prostate cancer cases from normal cases have confirmed a good accuracy of results. The presented methodology is of potential use for practical application in bioinformatics.
Źródło:
Bulletin of the Polish Academy of Sciences. Technical Sciences; 2012, 60, 3; 461-470
0239-7528
Pojawia się w:
Bulletin of the Polish Academy of Sciences. Technical Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Novel isoforms of transcript of the EDA gene confirm X-linked inheritance of anhidrotic ectodermal dysplasia
Autorzy:
Kobielak, K
Kobielak, A.
Trzeciak, W.H.
Powiązania:
https://bibliotekanauki.pl/articles/2043606.pdf
Data publikacji:
1999
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
dysplasia
inheritance
anhidrotic ectodermal dysplasia
Christ-Siemens-Touraine syndrome
Tabby gene
EDA gene
gene transcript
Źródło:
Journal of Applied Genetics; 1999, 40, 4; 355-364
1234-1983
Pojawia się w:
Journal of Applied Genetics
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Properties of a Singular Value Decomposition Based Dynamical Model of Gene Expression Data
Autorzy:
Simek, K.
Powiązania:
https://bibliotekanauki.pl/articles/908156.pdf
Data publikacji:
2003
Wydawca:
Uniwersytet Zielonogórski. Oficyna Wydawnicza
Tematy:
informatyka
multiple gene expression
singular value decomposition
dynamical model of gene expression data
Opis:
Recently, data on multiple gene expression at sequential time points were analyzed using the Singular Value Decomposition (SVD) as a means to capture dominant trends, called characteristic modes, followed by the fitting of a linear discrete-time dynamical system in which the expression values at a given time point are linear combinations of the values at a previous time point. We attempt to address several aspects of the method. To obtain the model, we formulate a nonlinear optimization problem and present how to solve it numerically using the standard MATLAB procedures. We use freely available data to test the approach. We discuss the possible consequences of data regularization, called sometimes "polishing", on the outcome of the analysis, especially when the model is to be used for prediction purposes. Then, we investigate the sensitivity of the method to missing measurements and its abilities to reconstruct the missing data. Summarizing, we point out that approximation of multiple gene expression data preceded by SVD provides some insight into the dynamics, but may also lead to unexpected difficulties, like overfitting problems.
Źródło:
International Journal of Applied Mathematics and Computer Science; 2003, 13, 3; 337-345
1641-876X
2083-8492
Pojawia się w:
International Journal of Applied Mathematics and Computer Science
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
SSCP polymorphism within 5 region of bovine lactoglobulin [LGB] gene
Autorzy:
Kaminski, S
Zabolewicz, T.
Powiązania:
https://bibliotekanauki.pl/articles/2044462.pdf
Data publikacji:
1998
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
lactoglobulin gene
polymorphism
SSCP method
cattle
gene transcription
electrophoresis
hormonal receptor
mutation
beta-lactoglobulin
milk
Opis:
In the paper the detection of the SSCP polymorphism within the 5’ fragment of bovine beta-lactoglobulin (LGB) gene is described. The 5’ fragment of LGB gene (209 bp) was PCR-amplified and then subjected to electrophoresis allowing the detection of SSCP polymorphism. Among 124 animals (50 cows and 74 bulls) six SSCP patterns were identified and named Rl, R2, R3, R4, R5 and R6, which occured with the frequency of 0.32, 0.51, 0.09, 0.06, 0.01 and 0.01, respectively. The PCR-SSCP method is simple, fast, and relatively inexpensive. The SSCP polymorphism reported in the paper may be useful in looking for the associations between different SSCP patterns and LGB gene expression and milk properties.
Źródło:
Journal of Applied Genetics; 1998, 39, 1; 97-102
1234-1983
Pojawia się w:
Journal of Applied Genetics
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The effect of phenobarbital on gene expression levels of p53 and DNMT1 in the liver of Wistar rats
Autorzy:
Urbanek-Olejnik, K.
Liszewska, M.
Kostka, G.
Powiązania:
https://bibliotekanauki.pl/articles/874211.pdf
Data publikacji:
2014
Wydawca:
Narodowy Instytut Zdrowia Publicznego. Państwowy Zakład Higieny
Tematy:
phenobarbital
gene expression
animal exposure
rat
p53 gene
Dnmt1 gene
liver
Wistar rat
Opis:
Background. Our previous studies have shown that short-term treatment with phenobarbital (PB) resulted in cytosine methylation of CpG sites on the p53 gene promoter in male Wistar rats’ liver. Furthermore, PB induced DNA-methyltransferases (DNMTs) activity was also demonstrated; being the enzymes that catalyze methyl group transfer to cytosine in CpG dinucleotides. Objective. Since DNA methylation is involved in regulating gene transcription and that DNMT1 is implicated in regulating DNA methylation, this study assessed whether PB-induced hypermethylation of the p53 promoter region was associated with an altered expression of p53 and Dnmt1 genes. Material and methods. Male Wistar rats received PB in three daily oral doses (at 24-h intervals) of 92,8 mg/kg b.w. x day-1. Levels of mRNA for p53 and Dnmt1 and levels of relevant proteins were respectively examined by Real-Time PCR and Western blot analysis. Results. Gene expression analysis revealed that exposure of Wistar rats to PB caused statistically significant alternations in the expression of tested genes. We found that both mRNA and protein expression of p53 was down-regulated, whereas expression of Dnmt1 (both mRNA and protein) was up-regulated after PB treatment. Conclusions. Suppression of p53 mRNA and protein expression, which is probably a result of epigenetic changes, (in particular aberrant p53 promoter hypermethylation), can be associated with tumour promoting activity of phenobarbital.
Wprowadzenie. Nasze wcześniejsze badania wykazały, że krótkoterminowe narażenie szczurów Wistar na fenobarbital (PB) stymulowało metylację cytozyny w badanych sekwencjach rejonu promotorowego genu p53. Ponadto stwierdzono wzrost aktywności metylotransferaz DNA (DNMT), enzymów które katalizują przenoszenie grupy metylowej do cytozyny w dinukleotydach CpG. Cel badań. Z uwagi że metylacja DNA pełni istotną rolę w ekspresji genów, a DNMT1 uczestniczy w regulacji metylacji DNA, w prezentowanych badaniach oceniano czy indukowana PB hipermetylacja rejonu promotorowego genu p53 była związana ze zmianami ekspresji genów p53 i Dnmt1. Materiał i metody. Samce szczurów szczepu Wistar otrzymywały PB w dawce 92,8 mg/kg m.c. x dzień-1, 3-krotnie w odstępach dobowych. Analizę poziomu transkryptów i białek badanych genów przeprowadzano odpowiednio metodą Real- -Time PCR i Western blot. Wyniki. W wyniku oddziaływania PB wykazano obniżoną ekspresję genu p53 i wzrost ekspresji metylotranserazy 1 (DNMT1). Wnioski. Supresja ekspresji p53 (na poziomie mRNA i białka) będąca prawdopodobnie wynikiem zmian epigenetycznych, w szczególności hipermetylacji jego rejonu promotorowego może być związana z promocyjną aktywnością fenobarbitalu.
Źródło:
Roczniki Państwowego Zakładu Higieny; 2014, 65, 3
0035-7715
Pojawia się w:
Roczniki Państwowego Zakładu Higieny
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Search for mouse gene related to GA733 human tumor antigen gene
Autorzy:
Zielewicz, J
Skrzypczak, M.
Wojcierowski, J.
Powiązania:
https://bibliotekanauki.pl/articles/2048195.pdf
Data publikacji:
1995
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
human antigen
glycoprotein
human genetics
mice
gene sequence
adenocarcinoma
cancer therapy
electrophoresis
carcinoma cell
bacteriophage
antigen
gene
mouse
oncoantigen
hybridization
tumour etiology
tumour
DNA
Opis:
Human antigen GA733-1, defined as 40 kDa cell glycoprotein, is one of the antigens associated with gastrointestinal carcinomas. Its studies may contribute to the tumor etiology and therapy effects in animal model.
Źródło:
Journal of Applied Genetics; 1995, 36, 3; 273-277
1234-1983
Pojawia się w:
Journal of Applied Genetics
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Direct transfer of IL-12 gene into growing Renca tumors.
Autorzy:
Budryk, Magdalena
Wilczyńska, Urszula
Szary, Jarosław
Szala, Stanisław
Powiązania:
https://bibliotekanauki.pl/articles/1044365.pdf
Data publikacji:
2000
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
IL-12 gene
tumor gene therapy
naked DNA
Opis:
We investigated the feasibility of transferring naked plasmid DNA containing a therapeutic gene (IL-12) into mice harboring growing Renca tumors. We found that naked DNA transferred into growing Renca and B16(F10) tumors gives higher expression level of reporter gene than complexes of DNA with DDAB/ DOPE or DC-Chol/DOPE. Transfer of naked DNA carrying the IL-12 gene into growing Renca tumors causes a distinct therapeutic effect that depends on the time span between inoculation of mice with cancer cells and the beginning of the therapy. Therapy started on day 3 resulted in total cure (100%) of mice.
Źródło:
Acta Biochimica Polonica; 2000, 47, 2; 385-391
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Novel regulators of photosynthesis
Autorzy:
Leister, D.
Powiązania:
https://bibliotekanauki.pl/articles/80943.pdf
Data publikacji:
2013
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
conference
photosynthesis
chloroplast
gene expression
nuclear protein
nuclear gene
protein
thylakoid protein
Źródło:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2013, 94, 2
0860-7796
Pojawia się w:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Involvement of plastid, mitochondrial and nuclear genomes in plant-to-plant horizontal gene transfer
Autorzy:
Sanchez-Puerta, M.V.
Powiązania:
https://bibliotekanauki.pl/articles/57778.pdf
Data publikacji:
2014
Wydawca:
Polskie Towarzystwo Botaniczne
Tematy:
angiosperm
plastid
mitochondrial genome
nuclear genome
genome
horizontal gene transfer
gene conversion
parasite
Opis:
This review focuses on plant-to-plant horizontal gene transfer (HGT) involving the three DNA-containing cellular compartments. It highlights the great incidence of HGT in the mitochondrial genome (mtDNA) of angiosperms, the increasing number of examples in plant nuclear genomes, and the lack of any convincing evidence for HGT in the well-studied plastid genome of land plants. Most of the foreign mitochondrial genes are non-functional, generally found as pseudogenes in the recipient plant mtDNA that maintains its functional native genes. The few exceptions involve chimeric HGT, in which foreign and native copies recombine leading to a functional and single copy of the gene. Maintenance of foreign genes in plant mitochondria is probably the result of genetic drift, but a possible evolutionary advantage may be conferred through the generation of genetic diversity by gene conversion between native and foreign copies. Conversely, a few cases of nuclear HGT in plants involve functional transfers of novel genes that resulted in adaptive evolution. Direct cell-to-cell contact between plants (e.g. host-parasite relationships or natural grafting) facilitate the exchange of genetic material, in which HGT has been reported for both nuclear and mitochondrial genomes, and in the form of genomic DNA, instead of RNA. A thorough review of the literature indicates that HGT in mitochondrial and nuclear genomes of angiosperms is much more frequent than previously expected and that the evolutionary impact and mechanisms underlying plant-to-plant HGT remain to be uncovered.
Źródło:
Acta Societatis Botanicorum Poloniae; 2014, 83, 4
0001-6977
2083-9480
Pojawia się w:
Acta Societatis Botanicorum Poloniae
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Helper-dependent adenoviral vectors in experimental gene therapy
Autorzy:
Józkowicz, Alicja
Dulak, Józef
Powiązania:
https://bibliotekanauki.pl/articles/1041362.pdf
Data publikacji:
2005
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
helper-dependent adenoviral vectors
adenoviruses
gene therapy
Opis:
In the majority of potential applications gene therapy will require an effective transfer of a transgene in vivo resulting in high-level and long-term transgene expression, all in the absence of significant toxicity or inflammatory responses. The most efficient vehicles for delivery of foreign genes to the target tissues are modified adenoviruses. Adenoviral vectors of the first generation, despite the high infection efficacy, have an essential drawback: they induce strong immune response, which leads to short term expression of the transgene, and limits their usefulness in clinical trials. In contrast, helper-dependent adenoviral vectors (HdAd) lacking all viral coding sequences display only minimal immunogenicity and negligible side-effects, allowing for long-term transgene expression. Thus, HdAd vehicles have become the carrier of choice for adenoviral vector-mediated experimental gene therapy, effectively used in animal models for delivery of transgenes into the liver, skeletal muscle, myocardium or brain. Strong and long-lasting expression of therapeutic genes has allowed for successful treatment of dyslipidemias, muscular dystrophy, obesity, hemophilia, and diabetes. Additionally, the large cloning capacity of HdAd, up to 37 kb, facilitates the use of physiologically regulated, endogenous promoters, instead of artificial viral promoter sequences. This enables also generation of the single vectors expressing multiple genes, which can be potentially useful for treatment of polygenic diseases. In this review we characterize the basic features of HdAd vectors and describe some of their experimental and potential clinical applications.
Źródło:
Acta Biochimica Polonica; 2005, 52, 3; 589-599
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
A structural gene – evolving term and dilemmas
Autorzy:
Chorąży, Mieczysław
Powiązania:
https://bibliotekanauki.pl/articles/704374.pdf
Data publikacji:
2009
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
DNA
structural gene
RNA transcripts
non-protein coding RNA
“secondary” information
Opis:
The term “gene” was originally used as a purely theoretical concept. After discovery of DNA structure, and understanding the genetic code, the gene acquired a form of a distinct physical entity with its borders and specific signal sequences, having rather simple (as it was thought at that time) functions and relation to phenotype outcome. The term “structural gene” has been coined. The unique gene structure, and several unusual and omnipotent traits have been ascribed to the gene that resulted in the formulation of a “genocentric” theory as a basic expla nation of all features of living organisms. However, recent discoveries reveal a complex structure and functions of eukaryotic genes. It appears now that the coding sequences (exons) are spread out over extended regions (hundreds of thousands of kilobases) of DNA. The role of protein-non coding DNA sequences were recognized, and the new mechanisms controlling gene functions have been discovered. In addition, we acquired the knowledge about a powerful ability of the cell to interpret the information potential of genes, accordingly to the needs of a cell/organ or actual “context” and status of the dynamic systems operating within the cell. All these discoveries undermine the genocentric view of life. At this time any definition of “gene” seems to be inadequate with present knowledge, and one may ask again: what is a gene?
Źródło:
Nauka; 2009, 3
1231-8515
Pojawia się w:
Nauka
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
ERF022 controls somatic embryogenesis in Arabidopsis via ethylene-related mechanism
Autorzy:
Nowak, K.
Gaj, M.D.
Powiązania:
https://bibliotekanauki.pl/articles/80300.pdf
Data publikacji:
2013
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
conference
gene encoding
transcription factor
somatic embryogenesis
ERF022 gene
Arabidopsis
expression pattern
tf gene
plant stress
ethylene biosynthesis
Źródło:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2013, 94, 3
0860-7796
Pojawia się w:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Association between RBMS1 gene rs7593730 and BCAR1 gene rs7202877 and Type 2 diabetes mellitus in the Chinese Han population
Autorzy:
Kazakova, Elena
Chen, Meijun
Jamaspishvili, Esma
Lin, Zhang
Yu, Jingling
Sun, Lulu
Qiao, Hong
Powiązania:
https://bibliotekanauki.pl/articles/1038363.pdf
Data publikacji:
2018
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
type 2 diabetes mellitus
RBMS1 gene
BCAR1 gene
single nucleotide polymorphisms
SNPscan
Opis:
Two recent studies found that RBMS1 gene rs7593730 and BCAR1 gene rs7202877 are related to type 2 diabetes. However, the association of these loci with type 2 diabetes mellitus (T2DM) has not been examined in Chinese. We performed a replication study to investigate the association of the 2 susceptibility loci with T2DM in the Chinese population. We genotyped 1961 Chinese participants (991 with T2DM and 970 controls) for each of the 2 single nucleotide polymorphisms (SNPs) rs7593730 in RBMS1 and rs7202877 near BCAR1 using SNPscan and examined their association with T2DM using logistic regression analysis. We also analyzed the correlation of the SNP alleles and clinical phenotypes. In additive model, genotype association analysis of BCAR1 rs7202877 loci revealed that the homozygous of rs7202877 GG carriers had significantly decreased T2DM risk compared to homozygous carriers of TT (P=0.038, OR 0.44, 95% CI 0.20-0.96). In the recessive model, the GG genotype GG had significantly decreased T2DM risk compared to GT+TT (P=0.043, OR 0.67, 95% CI 0.46-0.99). Allele G was statistically significantly correlated with TC (mmol/L) (P=0.036) and LDL-C (mmol/L) (P=0.007). As for rs7593730, the carriers of CT and TT genotype had significantly decreased T2DM risk compared to the carriers of CC genotype (CT: CC P=0.038, OR 0.71, 95% CI 0.51-0.98; TT: CC P=0.010, OR 0.32, 95% CI 0.13-0.76). In a dominant model, TT+CT: CC (P=0.013, OR 0.673, 95% CI 0.49-0.92) and in a recessive model, TT: CT+CC (P=0.019, OR 0.59, 95% CI 0.39-0.92). The T allele carriers had significantly decreased T2DM risk compared to the carriers of C (P=0.002, OR 0.65, 95% CI 0.50-0.86). Allele T was statistically correlated with FINS (P=0.010). In conclusion, our study showed that RBMS1 gene rs7593730 and BCAR1 gene rs7202877 were significantly associated with type 2 diabetes in the Chinese population.
Źródło:
Acta Biochimica Polonica; 2018, 65, 3; 377-382
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Bounded Picard groups
Autorzy:
Abrams, Gene
Haefner, Jeremy
Powiązania:
https://bibliotekanauki.pl/articles/966672.pdf
Data publikacji:
1997
Wydawca:
Polska Akademia Nauk. Instytut Matematyczny PAN
Źródło:
Colloquium Mathematicum; 1997, 72, 2; 325-334
0010-1354
Pojawia się w:
Colloquium Mathematicum
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
More Acanthamoeba Genotypes: Limits to Use rDNA Fragments to Describe New Genotype
Autorzy:
Corsaro, Daniele
Powiązania:
https://bibliotekanauki.pl/articles/763668.pdf
Data publikacji:
2011
Wydawca:
Uniwersytet Jagielloński. Wydawnictwo Uniwersytetu Jagiellońskiego
Tematy:
Acanthamoeba, genotype, full gene sequence, partial gene sequence
Opis:
Strains of the genus Acanthamoeba are usually assigned to sequence types or genotypes according to pair-wise similarity values of the nuclear gene for the small subunit of ribosomal RNA. This classification system was established by comparing full or nearly full gene sequences, > 2000 bp. For practical reasons, diagnostic fragments of smaller lengths have been identified and used for rapid and economic identification of large number of strains. While the use of these small fragments in diagnostics applications remains valid when and only if the reference full sequence-type is available, we contest their use to identify and describe new genotypes. We report herein the case of a new genotype described on the basis of solely a small partial sequence and discuss the poor reliability of this fragment to correctly infer phylogenetic relationships, and its limits in the description of new genotypes of Acanthamoeba.
Źródło:
Acta Protozoologica; 2011, 50, 1
1689-0027
Pojawia się w:
Acta Protozoologica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Genetic predisposition to breast and/or ovarian cancer – focus on the candidate BARD1 gene
Autorzy:
Klonowska, K.
Ratajska, M.
Wojciechowska, M.
Kozlowski, P.
Powiązania:
https://bibliotekanauki.pl/articles/80894.pdf
Data publikacji:
2014
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
breast cancer
ovarian cancer
genetic predisposition
BRCA1 gene
structure
function
BARD1 gene
Źródło:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2014, 95, 3
0860-7796
Pojawia się w:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Analysis of dynamics of gene exspression using singular value decomposition
Autorzy:
Simek, K.
Kimmel, M.
Powiązania:
https://bibliotekanauki.pl/articles/332865.pdf
Data publikacji:
2002
Wydawca:
Uniwersytet Śląski. Wydział Informatyki i Nauki o Materiałach. Instytut Informatyki. Zakład Systemów Komputerowych
Tematy:
wielokrotna ekspresja genów
dynamiczny model danych ekspresji genów
pojedynczy rozkład wartości
multiple gene expression
dynamic model of gene expression data
singular value decomposition
Opis:
Recently, data on multiple gene expression at sequential time points were analyzed, using Singular Value Decomposition (SVD) as a means to capture dominant trends, called characteristic modes, followed by fitting of a linear discrete-time dynamical model in which the expression values at a given time point are linear combinations of the values at a previous time point. We attempt to address several aspects of the method. To obtain the model we formulate a nonlinear optimization problem and present how to solve it numerically using standard MATLAB procedures. We use publicly available data to test the approach. Then, we investigate the sensitivity of the method to missing measurements and its possibilities to reconstruct missing data. Summarizing we point out that approximation of multiple gene expression data preceded by SVD provides some insight into the dynamics but may also lead to unexpected difficulties.
Źródło:
Journal of Medical Informatics & Technologies; 2002, 3; MI31-40
1642-6037
Pojawia się w:
Journal of Medical Informatics & Technologies
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Characterization of the suppressor gene of powdery mildew resistance gene Pm8 in common wheat [Triticum aestivum L.] cv. Regina
Autorzy:
Hanusova, R
Bartos, P
Zeller, F J
Powiązania:
https://bibliotekanauki.pl/articles/2046597.pdf
Data publikacji:
1997
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
yellow rust resistance
resistance gene Pm8
powdery mildew
wheat
suppressor gene
leaf rust resistance
Triticum aestivum
stem rust resistance
Opis:
The Czech winter wheat cv. Regina which does not possess specific genes for powdery mildew resistance except Pm5 was crossed with the cvs. Florida, Tjelvar, Agra, Olymp and Sabina, all possessing T1BL-1RS and the dominant suppressor SuPm8, with Riebesel 47/51 possessing substitution 1R-1B and SuPm8 and with the T1BL-1RS cvs. Sparta, Iris, Mona without SuPm8. Powdery mildew isolates avirulent on Pm8 and virulent on other Pm genes were used in the trials. Tests for resistance were carried out either on seedlings at the first leaf stage or on detached leaves cultured on benzimidazolagar. No segregation for resistant plants in F₂ of all crosses of the cv. Regina with cultivars possessing T1BL-1RS and SuPm8 indicates that Regina has SuPm8. The segregation ratio 3 resistant: 13 susceptible in the F₂ population of crosses between the cv. Regina and cultivars possessing T1BL-1RS and no SuPm8 also confirms the presence of SuPm8 in the cv. Regina. The obtained results also indicate that expression of Pm2 and Pm4b is not affected by SuPm8 and that SuPm8 does not affect stem rust resistance gene Sr31 located on 1RS segment.
Źródło:
Journal of Applied Genetics; 1997, 38, 1; 11-17
1234-1983
Pojawia się w:
Journal of Applied Genetics
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Gene patents – short report
Autorzy:
Waszkowska, E.
Powiązania:
https://bibliotekanauki.pl/articles/80750.pdf
Data publikacji:
2014
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Źródło:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2014, 95, 4
0860-7796
Pojawia się w:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Involvement of the essential yeast DNA polymerases in induced gene conversion
Autorzy:
Hałas, Agnieszka
Ciesielski, Arkadiusz
Żuk, Jerzy
Powiązania:
https://bibliotekanauki.pl/articles/1044442.pdf
Data publikacji:
1999
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
gene conversion
DNA polymerases
Saccharomyces cerevisiae
Opis:
In the yeast Saccharomyces cerevisiae three different DNA polymerases α, δ and ε are involved in DNA replication. DNA polymerase α is responsible for initiation of DNA synthesis and polymerases δ and ε are required for elongation of DNA strand during replication. DNA polymerases δ and ε are also involved in DNA repair. In this work we studied the role of these three DNA polymerases in the process of recombinational synthesis. Using thermo-sensitive heteroallelic mutants in genes encoding DNA polymerases we studied their role in the process of induced gene conversion. Mutant strains were treated with mutagens, incubated under permissive or restrictive conditions and the numbers of convertants obtained were compared. A very high difference in the number of convertants between restrictive and permissive conditions was observed for polymerases α and δ, which suggests that these two polymerases play an important role in DNA synthesis during mitotic gene conversion. Marginal dependence of gene conversion on the activity of polymerase ε indicates that this DNA polymerase may be involved in this process but rather as an auxiliary enzyme.
Źródło:
Acta Biochimica Polonica; 1999, 46, 4; 862-872
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Is there any mystery of ORPHANs?
Autorzy:
Cebrat, S
Dudek, M
Mackiewicz, P
Powiązania:
https://bibliotekanauki.pl/articles/2046624.pdf
Data publikacji:
1997
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
yeast
gene finding
DNA asymmetry
orphan
gene number
genome
Saccharomyces cerevisiae
Opis:
We have analysed the coding capacity of ORFs longer than 100 codons found in the yeast genome. Comparing the parameters describing the DNA asymmetry in the set of known genes and the set of all ORFs>100 codons we have found that there are about 4700 coding ORFs in the yeast genome. Since for more than 2300 ORFs recognisable functions have been already found and for about 2000 ORFs homology to known genes has been identified - only about 400 ORFs can be considered as orphans - ORFs without any known function or homology. This finding means that there is no mystery of orphans - a paradox showing that the fraction of orphans has been growing with the growing number of genes with known functions in the yeast genome.
Źródło:
Journal of Applied Genetics; 1997, 38, 4; 365-372
1234-1983
Pojawia się w:
Journal of Applied Genetics
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
A comprehensive in silico prediction of the most deleterious missense variants in the bovine LEP gene
Autorzy:
Al-Shuhaib, M.B.S.
Powiązania:
https://bibliotekanauki.pl/articles/80824.pdf
Data publikacji:
2019
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
LEP gene
leptin
biological activity
bovine gene
single nucleotide polymorphism
coding sequence
cattle
Źródło:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2019, 100, 4
0860-7796
Pojawia się w:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Telomeraza – struktura i funkcja oraz regulacja ekspresji genu
Telomerase – structure, function and the regulation of gene expression
Autorzy:
Bryś, Magdalena
Laskowska, Magdalena
Forma, Ewa
Krześlak, Anna
Powiązania:
https://bibliotekanauki.pl/articles/1032770.pdf
Data publikacji:
2012
Wydawca:
Łódzkie Towarzystwo Naukowe
Tematy:
telomeraza
gen
białko
ekspresja genu
czynniki
transkrypcyjne
telomerase
gene
protein
gene expression
transcription factors
Opis:
A telomere is a fragment localized at the end of chromosome which protects the chromosome from damage during replication. Telomeres are also factors that control number of cell divisions and are thought to be a suppressors of carcinogenesis since limited, strictly determined number of cell divisions protects from accumulation of mutations in cell. It is assumed that presence of 4-6 mutation in genetic material is a carcinogenic factor and after about 60-70 divisions, the cell enter the resting phase. Telomerase is an enzyme which adds DNA sequence to the 3’ end of DNA and extends the telomere region. This protein is a DNA polymerase dependent on RNA, which syntheses telomere by reverse transcription. The unique characteristics of telomerase is that RNA matrix for DNA synthesis is an integral component of this enzyme. Telomerase is present in intensively dividing cells and its activity is decreasing with age. In normal cells usually activity of telomerase is undetectable but in cancer cells activity of this enzyme is high. The aim of this work is to present the structure of telomeres and the role of proteins involved in maintaining the structure. In details, the structure and function of the telomerase gene/protein is described, including the regulation of gene expression at the transcriptional level. The involvement of telomerase in the neoplastic transformation has been also characterized.
Telomer jest to fragment chromosomu zlokalizowany na jego końcu, który zabezpiecza go przed uszkodzeniem podczas kopiowania. Telomery są także czynnikami kontrolującymi liczbę podziałów komórkowych i dlatego uważane są za supresory transformacji nowotworowej, ponieważ ograniczona, ściśle kontrolowana liczba podziałów zapobiega ewentualnemu kumulowaniu się mutacji w komórce. Przyjęto, że obecność 4-6 mutacji w materiale genetycznym jest czynnikiem karcynogennym, a po granicznej liczbie podziałów (około 60-70) komórka wchodzi w fazę spoczynku M1. Enzymem, którego zadaniem jest dobudowanie 3'-końcowego odcinka nici DNA i tym samym wydłużanie sekwencji telomerowych jest enzym telomeraza. Białko to jest polimerazą DNA zależną od RNA, która syntetyzuje telomery na zasadzie odwrotnej transkrypcji. Unikalną cechą telomerazy jest to, że jej integralnym składnikiem jest matryca RNA służąca do syntezy DNA. Telomeraza występuje w intensywnie dzielących się komórkach, a jej aktywność zmniejsza się wraz z wiekiem. W komórkach prawidłowych zwykle nie stwierdza się aktywności telomerazy, natomiast w nowotworowych aktywność tego enzymu zwykle jest podwyższona. W pracy omówiono strukturę sekwencji telomerowych oraz udział białek zaangażowanych w jej utrzymanie. Szczegółowo przedstawiono także strukturę i funkcję genu/białka telomerazy, z uwzględnieniem regulacji ekspresji genu na poziomie transkrypcji. Scharakteryzowano ponadto udział telomerazy w procesach transformacji nowotworowej.
Źródło:
Folia Medica Lodziensia; 2012, 39, 2; 293-326
0071-6731
Pojawia się w:
Folia Medica Lodziensia
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Combined delivery of an antiangiogenic protein (angiostatin) and an immunomodulatory gene (interleukin-12) in the treatment of murine cancer.
Autorzy:
Wilczyńska, Urszula
Kucharska, Anna
Szary, Jarosław
Szala, Stanisław
Powiązania:
https://bibliotekanauki.pl/articles/1044050.pdf
Data publikacji:
2001
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
angiogenesis
IL-12 gene
tumor gene therapy
angiostatin
Opis:
We investigated the feasibility of a novel therapeutic approach to treat neoplastic diseases in mice. This novel strategy consists in delivering a protein (angiostatin) with strong antiangiogenic properties, followed by administration of the interleukin 12 gene that is strongly immunomodulatory and has also some antiangiogenic effects. When angiostatin-mediated antiangiogenic therapy was used in combination with intratumor delivery of the IL-12 gene (a strategy much safer than IL-12 protein administration), this produced a synergistic therapeutic effect.
Źródło:
Acta Biochimica Polonica; 2001, 48, 4; 1077-1084
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Der Geist und sein Denken. Zur Rezeption der platonischen megista-gene Lehre bei Plotin und Proklos
The Spirit and Its Thinking. On the Recepcion of Plato’s megista gēnē Doctrine in Plotinus and Proclus
Autorzy:
Gögelein, Simon
Powiązania:
https://bibliotekanauki.pl/articles/938385.pdf
Data publikacji:
2014
Wydawca:
Uniwersytet im. Adama Mickiewicza w Poznaniu
Tematy:
Plato
megista gēnē
Plotinus
Proclus
Opis:
This article is primarily concerned with Platoʼs later dialogue, the Sophist, and the reception of the megista-gēnē-dialectic in Neoplatonism (especially Plotinus and Proclus). The present paper offers a historical comparative study that consists of three parts. The first one gives a short summary concerning Platoʼs request regarding the concept of inverse and complex Ideas. The second one examines Plotinus’ conception of the νοῦς (Enn. VI 2, 7–8), in which the megista gēnē στάσις, κίνησις, ὄν, ταὐτόν and ἕτερον constitute the realm of the intellect. While the third and final part of the article investigates Proclusʼ extrapolation of the Platonic dialectic, it focuses on selected passages from the Commentary on the Parmenides. The paper concludes with a summary of the results.
Źródło:
Peitho. Examina Antiqua; 2014, 5, 1; 139-162
2082-7539
Pojawia się w:
Peitho. Examina Antiqua
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Analysis of occurrence of virulence genes among Yersinia enterocolitica isolates belonging to different biotypes and serotypes
Autorzy:
Kot, B
Piechota, M.
Jakubczak, A.
Powiązania:
https://bibliotekanauki.pl/articles/32221.pdf
Data publikacji:
2010
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
virulence gene
occurrence
Yersinia enterocolitica
isolate
biotype
serotype
polymerase chain reaction
ystB gene
myfA gene
man
pig
isolation
Opis:
The 150 Y. enterocolitica strains isolated from humans and from pigs belonged to biotypes 4 (68.7%), 1A (18.7%) and 2 (4%), or were biochemically untypeable (8.6%). Biotype 4 was comprised of Y. enterocolitica strains representing serotype 0:3, within biotype 1A the strains either belonged to serotypes 0:5 and 0:6 or were untypeable, and biotype 2 was represented by the strains of serotype 0:9. The strains which were biochemically untypeable belonged to serotypes 0:5, 0:6 and 0:3. Among the strains tested there also were those of an unidentified biotype and serotype. Nearly all the strains of biotype 1A represented genotype ystB+myfA+, and few belonged to genotype ystB+. The presence of the ystB gene in the strains of biotype 1A and only occasional occurrence of the gene in the other biotypes makes ystB a distinguishing marker of biotype 1A. The strains of genotype ystA+ail+myfA+yadA+ predominated in biotype 4 (serotype 0:3). The strains of biotype 2 (serotype 0:9) represented genotype ystA+ail+myfA+, and the plasmid yadA gene was detected in some of them. Within the group of biochemically untypeable strains ystB- and myfA-specific PCR products were mainly obtained. The genotypes determined for the tested biotypes and serotypes of Y. enterocolitica, based upon the selected genes of virulence, can be applied as distinguishing markers and indicators of the potential virulence of Y. enterocolitica strains, excluding bioserotyping.
Źródło:
Polish Journal of Veterinary Sciences; 2010, 13, 1; 13-19
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The uselessness of cytochrome oxydase gene analysis for genetic identification of selected Gobiidae species from some European countries
Autorzy:
Kiełpińska, Jolanta
Czerniejewski, Przemysław
Adamkowska, Natalia
Uzunova, Eliza
Powiązania:
https://bibliotekanauki.pl/articles/2203561.pdf
Data publikacji:
2022
Wydawca:
Instytut Technologiczno-Przyrodniczy
Tematy:
cytochrome oxidase subunit 1 gene
COI gene
Gobiidae
invasive species
native species
mt DNA
Opis:
The family Gobiidae is the largest teleost family, consisting of more than 2,000 species in more than 200 genera. They have adapted to a wide range of habitats, inhabiting mostly marine and brackish waters. Recently invasive Ponto-Caspian gobies have caused significant changes to the structure of fish assemblage throughout the European waters. The aim of the study was to verify the possibility of identifying three species of the genus Gobiidae based on the analysis of the mitochondrial region of the cytochrome oxidase subunit 1 (COI) gene. 339 samples originated from seven countries and 17 different sites were investigated. Qualitative and quantitative assessment of the extracted DNA was conducted by measuring light absorbance and electrophoretic separation. Approximately 650-bp (base pairs) fragments were amplified from the 5' region of the mitochondrial COI with the PCR (polymerase chain reaction) reaction. Sequences of 652 bp were obtained and compared with reference sequences in the National Center for Biotechnology Information (NCBI). Based on the sequence analysis, we determined that in the analysed geographical regions, the goby preliminary identified morphologically as the round goby was represented by three species: round goby (Neogobius melanostomus), tubenose goby (Proterorhinus marmoratus), and monkey goby (Neogobius fluviatilis). The latter two are newly reported for their new habitats. Genetic characterisation of such populations offers the opportunity to assess their genetic stability, which provides agencies managing the aquatic environment in EU countries with a tool for revising the legislation, including regulations relevant for updating the status of native and invasive species in European waters.
Źródło:
Journal of Water and Land Development; 2022, Special Issue; 8--14
1429-7426
2083-4535
Pojawia się w:
Journal of Water and Land Development
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Molecular typing of Staphylococcus aureus based on PCR-RFLP of coa gene and RAPD analysis
Autorzy:
Karakulska, J.
Pobucewicz, A.
Nawrotek, P.
Muszynska, M.
Furowicz, A.J.
Czernomysy-Furowicz, D.
Powiązania:
https://bibliotekanauki.pl/articles/31963.pdf
Data publikacji:
2011
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
molecular typing
Staphylococcus aureus
PCR-RFLP method
coa gene
random amplified polymorphic DNA analysis
mastitis
nuc gene
gap gene
milk sample
species identification
Opis:
The aim of this study was molecular identification of S. aureus strains isolated from mastitic milk samples and establishing the genetic relationship between strains isolated from cows belonging to the same herd. In all 43 isolated strains the gap gene (930 bp) was amplified, which enabled their affiliation to the Staphylococcus genus to be established. PCR-RFLP with AluI endonuclease of the gap gene as well as nuc (450 bp) and coa (1130 bp) gene amplification allowed precise S. aureus species identification. One hundred percent of the genetic relationship between strains was established via RAPD-PCR and coa-typing.
Źródło:
Polish Journal of Veterinary Sciences; 2011, 14, 2
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Gene expression pattern in canine mammary osteosarcoma
Autorzy:
Pawlowski, K.
Majewska, A.
Szyszko, K.
Dolka, I.
Motyl, T.
Krol, M.
Powiązania:
https://bibliotekanauki.pl/articles/32248.pdf
Data publikacji:
2011
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
bone tumour
canine mammary osteosarcoma
gene expression
mammary osteosarcoma
microarray
osteosarcoma
transcriptome
tumour
sarcoma
proliferation
aggressive tumour
gene list
Opis:
Canine mammary sarcomas are usually very aggressive and easily metastasize. Unfortunately the biology of this type of tumor is not well known because they are a very rare type of tumors. The aim of this study was to find differences in gene expression patterns in canine mammary osteosarcomas (malignant) versus osteomas (benign) using DNA microarrays. Our microarray experiment showed that 11 genes were up-regulated in osteosarcoma in comparison to osteoma whereas 36 genes were down-regulated. Among the up-regulated genes were: PDK1, EXT1, and EIF4H which are involved in AKT/PI3K and GLI/Hedgehog pathways. These genes play an important role in cell biology (cancer cell proliferation) and may be essential in osteosarcoma formation and development. Analyzing the down-regulated genes, the most interesting seemed to be HSPB8 and SEPP1. HSPB8 is a small heat shock protein that plays an important role in cell cycle regulation, apoptosis, and breast carcinogenesis. Also SEPP1 may play a role in carcinogenesis, as its down-regulation may induce oxidative stress possibly resulting in carcinogenesis. The preliminary results of the present study indicate that the up-regulation of three genes EXT1, EIF4H, and PDK1 may play an essential role in osteosarcoma formation, development and proliferation. In our opinion the cross-talk between GLI/Hedgehog and PI3K/AKT pathways may be a key factor to increase tumor proliferation and malignancy.
Źródło:
Polish Journal of Veterinary Sciences; 2011, 14, 1
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Plasminogen activator inhibitor-1 (PAI-1) gene 4G/5G promoter polymorphism is not associated with breast cancer.
Autorzy:
Błasiak, Janusz
Smolarz, Beata
Powiązania:
https://bibliotekanauki.pl/articles/1044431.pdf
Data publikacji:
2000
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
gene polymorphism
PAI-1 gene
prognostic marker
plasminogen activator inhibitor-1 (PAI-1)
breast cancer
Opis:
The antigen content of plasminogen activator inhibitor-1 (PAI-1) in primary breast cancer tissue extracts may be of strong prognostic value: high levels of PAI-1 in tumors predict poor prognosis for patients. The gene encoding PAI-1 is highly polymorphic and an insertion (5G)/deletion (4G) polymorphism in the PAI-1 gene promoter (the 4G/5G polymorphism), may have functional significance in PAI-1 expression. In the present work the distribution of genotypes and frequency of alleles of the 4G/5G polymorphism in subjects with breast cancer were investigated. Tumor tissues were obtained from 100 postmenopausal women with node-negative and node-positive ductal breast carcinoma with uniform tumor size. Blood samples from age matched healthy women served as control. The 4G/5G polymorphism was determined by PCR amplification using the allele specific primers. The distribution of the genotypes of the 4G/5G polymorphism in both control and patients did not differ significantly (P > 0.05) from those predicted by the Hardy-Weinberg distribution. There were no differences in the genotype distributions and allele frequencies between node-positive and node-negative patients. The 4G/5G polymorphism may not be linked with elevated level of PAI-1 observed in breast cancer and therefore may not be associated with appearance and/or progression of breast cancer.
Źródło:
Acta Biochimica Polonica; 2000, 47, 1; 191-199
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Apoplastic ROS sensing and signalling
Autorzy:
Kangasjarvi, J.
Powiązania:
https://bibliotekanauki.pl/articles/80047.pdf
Data publikacji:
2013
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
conference
reactive oxygen species
signalling
plant cell
stress adaptation
acclimation
cysteine-rich protein
extracellular protein
gene regulation
marker gene
Źródło:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2013, 94, 2
0860-7796
Pojawia się w:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Climatological characterisation of the area of the Carpathian Regional Gene Bank in the Wisla Forest District
Autorzy:
Feliksik, E
Durlo, G.
Powiązania:
https://bibliotekanauki.pl/articles/41320.pdf
Data publikacji:
2004
Wydawca:
Polska Akademia Nauk. Instytut Dendrologii PAN
Tematy:
Wisla Forest District
Carpathian Regional Gene Bank
gene bank
climate
meteorology
Silesian Beskids Mountains
humidity
rainfall condition
thermal condition
Opis:
The paper summarises the results of climatological studies concerning the area of the Carpathian Regional Gene Bank station located in the Wisła Forest District. The work provides raw meteorological data, daily, monthly, seasonal and annual records, and various indices such as the pluvial-thermal index, biometeorological index, Sielianinow’s index and de Martonne’s index. In addition, the detailed profiles of thermal, humidity and rainfall conditions are presented against a background of the general synoptic situation.
Źródło:
Dendrobiology; 2004, 51 Supplement
1641-1307
Pojawia się w:
Dendrobiology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
CRH gene polymorphism in relation to milk production traits in cattle
Polimorfizm w genie CRH w powiązaniu z cechami użytkowości mlecznej bydła
Autorzy:
Kulig, H.
Kowalewska-Luczak, I.
Szydlowska, J.
Powiązania:
https://bibliotekanauki.pl/articles/44889.pdf
Data publikacji:
2011
Wydawca:
Zachodniopomorski Uniwersytet Technologiczny w Szczecinie. Wydawnictwo Uczelniane ZUT w Szczecinie
Tematy:
CRH gene
gene polymorphism
milk production trait
cattle
dairy cattle
Opis:
The aim of this study was to estimate the relations between the CRH-A145G polymorphism and milk production traits (yields of milk, protein, and fat, as well as protein and fat content) in 176 Jersey cows. The genotype and allele frequencies were estimated and they were as follows: AG – 0.31; GG – 0.69; A – 0.16; G – 0.84. Statistical analysis revealed that studied polymorphism significantly affected the fat yield, fat content (P≤0.05) and protein content in milk (P≤0.05). The results indicate that selection for the CRH-A145G AG animals might contribute to increase the value of these traits in Jersey cattle. However, further studies are necessary to verify the results of our study.
Celem badań było oszacowanie zależności między polimorfizmem CRH-A145G a cechami użytkowości mlecznej (wydajnością mleka, tłuszczu i białka oraz zawartością tłuszczu i białka w mleku) bydła. Badania przeprowadzono na stadzie 176 krów rasy jersey. Oszacowano częstość występowania genotypów i alleli, które wynosiły: AA – 0,00; AG – 0,31; GG – 0,69; A – 0,16; G – 0,84.Analiza statystyczna wykazała, że badany polimorfizm wpływał istotnie na wydajność tłuszczu i zawartość tłuszczu (P≤0,01) oraz na zawartość białka w mleku (P≤0,05).Wyniki wskazują, że uwzględnienie w selekcji osobników z genotypem CRH-A145G AG mogłoby przyczynić się do zwiększenia wartości powyższych cech u bydła rasy jersey. Wymagane jest jednak kontynuowanie badań aby móc zweryfikować uzyskane wyniki.
Źródło:
Acta Scientiarum Polonorum. Zootechnica; 2011, 10, 1
1644-0714
Pojawia się w:
Acta Scientiarum Polonorum. Zootechnica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
SURF1 gene mutations in Polish patients with COX-deficient Leigh syndrome
Autorzy:
Piekutowska-Abramczuk, D
Popowska, E.
Pronicka, E.
Karczmarewicz, E.
Pronicki, M.
Kmiec, T.
Krajewska-Walasek, M.
Powiązania:
https://bibliotekanauki.pl/articles/2041946.pdf
Data publikacji:
2001
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
Leigh disease
polymorphism
deletion
patient
substitution
Polska
SURF1 gene
gene mutation
Leigh syndrome
cytochrome oxidase
Źródło:
Journal of Applied Genetics; 2001, 42, 1; 103-108
1234-1983
Pojawia się w:
Journal of Applied Genetics
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Frequency of transgression and gene distribution in barley doubled haploid populations from first and second cycle hybrids
Autorzy:
Surma, M
Adamski, T.
Kaczmarek, Z.
Kapala, A.
Powiązania:
https://bibliotekanauki.pl/articles/2044239.pdf
Data publikacji:
1998
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
transgression
gene dispersion
doubled haploid
grain yield
hybrid
barley
first cycle hybrid
second cycle hybrid
recurrent selection
gene distribution
Opis:
Barley doubled haploids (DH) derived from first and second cycle hybrids were investigated in field experiments. Parental lines designed for the second cycle hybrids were three doubled haploids from the first cycle hybrids, which were observed to have the highest grain yield. Yield structure characters, crude protein content and protein fractions were analysed. Phenotypic and genetic variability and the frequency of transgression in the studied populations were calculated. For the studied traits additive, [d], and epistatic (additive x additive), [i], effects as well as coefficient of gene dispersion were estimated. It was found that the phenotypic and genetic variability of DH populations derived from second cycle hybrids was higher than that of the original population for all the studied traits except grain yield. A greater proportion of transgressive lines than in the original population was also observed in populations from second cycle hybrids. Only one DH line exceeding the high yielding parent was found among 141 lines under study. The relationship between the frequency of transgressions and gene dispersion was recorded: the greatest number of transgressive lines occurred in those traits for which the dispersion was observed.
Źródło:
Journal of Applied Genetics; 1998, 39, 3; 237-247
1234-1983
Pojawia się w:
Journal of Applied Genetics
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The association between ALR2 -106C > T gene polymorphisms and diabetic retinopathy susceptibility in diabetes mellitus patient: a systematic review and meta-analysis
Autorzy:
Putri, Indah Sagitaisna
Rezkita, Bastomy Eka
Irving, Steven
Azmiardi, Akhmad
Powiązania:
https://bibliotekanauki.pl/articles/4175390.pdf
Data publikacji:
2023-03-27
Wydawca:
Medical Education
Tematy:
ALR2 gene
diabetic retinopathy
diabetes mellitus
gene polymorphism
polyol pathway
Opis:
Aldose reductase gene polymorphisms has been indicated to be associated with diabetic retinopathy (DR). The research data were from PubMed and EMBASE. We identified -106C > T single nucleotide polymorphism (SNP). Pool odds ratio (OR) with 95% CI were calculated. Nine studies were included. ALR2 106C > T gene polymorphisms was associated with the increased risk of DR in T1DM (C vs. T, OR = 2.07, p = 0.001; CC vs. CT + TT, OR = 2.56, p = 0.005). T allele and TT genotype were associated with decreased risk of DR in T1DM (OR = 0.48, p = 0.0001 and OR = 0.12, p = 0.0005). In conclusion, C allele and CC genotype may be a risk factor, while T allele and TT genotype may serve as protective factor for DR in T1DM patient.
Źródło:
OphthaTherapy; 2023, 10, 1; 12-21
2353-7175
2543-9987
Pojawia się w:
OphthaTherapy
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Improved downstream process for the production of plasmid DNA for gene therapy
Autorzy:
Urthaler, Jochen
Buchinger, Wolfgang
Necina, Roman
Powiązania:
https://bibliotekanauki.pl/articles/1041381.pdf
Data publikacji:
2005
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
plasmid DNA
production
gene- therapy
purification
alkaline lysis
chromatography
Opis:
Gene therapy and genetic vaccines promise to revolutionize the treatment of inherited and acquired diseases. Since viral vectors are generally associated with numerous disadvantages when applied to humans, the administration of naked DNA, or DNA packed into lipo- or polyplexes emerge as viable alternatives. To satisfy the increasing demand for pharmaceutical grade plasmids we developed a novel economic downstream process which overcomes the bottlenecks of common lab-scale techniques and meets all regulatory requirements. After cell lysis by an in-house developed gentle, automated continuous system the sequence of hydrophobic interaction, anion exchange and size exclusion chromatography guarantees the separation of impurities as well as undesired plasmid isoforms. After the consecutive chromatography steps, adjustment of concentration and final filtration are carried out. The final process was proven to be generally applicable and can be used from early clinical phases to market-supply. It is scaleable and free of animal-derived substances, detergents (except lysis) and organic solvents. The process delivers high-purity plasmid DNA of homogeneities up to 98% supercoiled form at a high yield in any desired final buffer.
Źródło:
Acta Biochimica Polonica; 2005, 52, 3; 703-711
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Screening of the myelin protein zero gene in patients with Charcot-Marie-Tooth disease.
Autorzy:
Nowakowski, Adam
Kochański, Andrzej
Powiązania:
https://bibliotekanauki.pl/articles/1043357.pdf
Data publikacji:
2004
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
Charcot-Marie-Tooth disease
heteroduplex analysis
MPZ gene
SSCP
Opis:
The myelin protein zero gene (MPZ) coding for the most abundant protein of the peripheral myelin was shown to be mutated in Charcot-Marie-Tooth type 1B disease (CMT1B). Later on MPZ mutations have been shown in axonal type of CMT (CMT2). Recently three novel MPZ gene mutations were reported in congenital hypomyelinating neuropathy (CHN). In contrast to the previously reported studies, focused on CMT1B disease, we aimed to analyze the coding and promoter sequences of the MPZ gene in a group of patients with three CMT phenotypes i.e.: CMT1, CMT2 and CHN. Over 500 PCR products were screened by single strand conformation polymorphism analysis (SSCP) and heteroduplex analysis (HA). In one CMT2 family we founded the E56K mutation in the MPZ gene and in one CHN patient the T124K substitution was detected. In agreement with previously reported studies we conclude that MPZ gene screening should be performed for wide phenotype spectrum of CMT.
Źródło:
Acta Biochimica Polonica; 2004, 51, 1; 273-280
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
A rat testis-specific hsp70 gene-related transcript is coded by a novel gene from the hsp70 multigene family
Autorzy:
Krawczyk, Zdzisław
Wiśniewski, Jan
Biesiada, Elżbieta
Powiązania:
https://bibliotekanauki.pl/articles/1045870.pdf
Data publikacji:
1988
Wydawca:
Polskie Towarzystwo Biochemiczne
Źródło:
Acta Biochimica Polonica; 1988, 35, 4; 377-385
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Structural and functional genomics in domestic animals: the way to understand the phenotype
Autorzy:
Schwerin, M
Powiązania:
https://bibliotekanauki.pl/articles/2042006.pdf
Data publikacji:
2001
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
genome analysis
gene expression
candidate gene
farm animal
phenotype
livestock
domestic animal
Źródło:
Journal of Applied Genetics; 2001, 42, 3; 293-308
1234-1983
Pojawia się w:
Journal of Applied Genetics
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Hash function generation by means of Gene Expression Programming
Autorzy:
Varrette, S.
Muszyński, J.
Bouvry, P.
Powiązania:
https://bibliotekanauki.pl/articles/106148.pdf
Data publikacji:
2012
Wydawca:
Uniwersytet Marii Curie-Skłodowskiej. Wydawnictwo Uniwersytetu Marii Curie-Skłodowskiej
Tematy:
hash function
Gene Expression Programming
Genetic Programming
GEPHashSearch
cryptography
Opis:
Cryptographic hash functions are fundamental primitives in modern cryptography and have many security applications (data integrity checking, cryptographic protocols, digital signatures, pseudo random number generators etc.). At the same time novel hash functions are designed (for instance in the framework of the SHA-3 contest organized by the National Institute of Standards and Technology (NIST)), the cryptanalysts exhibit a set of statistical metrics (propagation criterion, frequency analysis etc.) able to assert the quality of new proposals. Also, rules to design "good" hash functions are now known and are followed in every reasonable proposal of a new hash scheme. This article investigates the ways to build on this experiment and those metrics to generate automatically compression functions by means of Evolutionary Algorithms (EAs). Such functions are at the heart of the construction of iterative hash schemes and it is therefore crucial for them to hold good properties. Actually, the idea to use nature-inspired heuristics for the design of such cryptographic primitives is not new: this approach has been successfully applied in several previous works, typically using the Genetic Programming (GP) heuristic [1]. Here, we exploit a hybrid meta-heuristic for the evolutionary process called Gene Expression Programming (GEP) [2] that appeared far more efficient computationally speaking compared to the GP paradigm used in the previous papers. In this context, the GEPHashSearch framework is presented. As it is still a work in progress, this article focuses on the design aspects of this framework (individuals definitions, fitness objectives etc.) rather than on complete implementation details and validation results. Note that we propose to tackle the generation of compression functions as a multi-objective optimization problem in order to identify the Pareto front i.e. the set of non-dominated functions over the four fitness criteria considered. If this goal is not yet reached, the first experimental results in a mono-objective context are promising and open the perspective of fruitful contributions to the cryptographic community.
Źródło:
Annales Universitatis Mariae Curie-Skłodowska. Sectio AI, Informatica; 2012, 12, 3; 37-53
1732-1360
2083-3628
Pojawia się w:
Annales Universitatis Mariae Curie-Skłodowska. Sectio AI, Informatica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Dde I RFLP at the 5 region of bovine kappa-casein gene
Autorzy:
Kaminski, S
Powiązania:
https://bibliotekanauki.pl/articles/2047278.pdf
Data publikacji:
1996
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
gene region
bovine kappa-casein
polymorphism
milk property
dairy cattle
Opis:
The bovine kappa-casein (CASK) gene is known as a potential quantitative trait locus in dairy cattle breeding. However, the molecular basis of the effect of the CASK allele B on different milk properties remains unclear. In this report, a 214 bp fragment of the 5' untranslated region of the CASK gene containing 5 potential consensus sequenses for different transcription factors was PCR-amplified to find RFLPs. A Dde I RFLP was identified. In a population of 112 Bos taurus (86 cows and 26 bulls of Polish Black and White crossbred Holstein-Friesian) and 7 Bison bonasus individuals, 7 had no recognition sites for Dde I, 23 were heterozygous and 89 were cut completely into two fragments.
Źródło:
Journal of Applied Genetics; 1996, 37, 2; 173-178
1234-1983
Pojawia się w:
Journal of Applied Genetics
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
On spins and genes
Autorzy:
Miękisz, Jacek
Szymańska, Paulina
Powiązania:
https://bibliotekanauki.pl/articles/748773.pdf
Data publikacji:
2012
Wydawca:
Polskie Towarzystwo Matematyczne
Tematy:
Ising model, self-regulating gene, mean-field approximation
Ising model, self-regulating gene, mean-field approximation.
Opis:
Naszym celem jest zrozumienie i przewidywanie zachowania się układów wielu oddziałujących obiektów, takich jak cząstki i spiny w fizyce statystycznej czy geny i białka w biologii molekularnej. Jako matematycy pragniemy udowadniać twierdzenia i wyprowadzać analityczne wzory. Bardzo szybko okazuje się, że w istotnych zastosowaniach jest to niemożliwe. Co robić? Część z nas ucieka w wyrafinowane symulacje komputerowe. Czy nie ma innej drogi? Czy jesteśmy ograniczeni do wyboru pomiędzy Matematyką i Mathematicą? Na pomoc przychodzi metoda samouzgodnionego pola średniego. Ferromagnetyczny model Isinga i samoregulujący się gen zilustrują nam tę niezwykle uniwersalną metodę otrzymywania przybliżonych rozwiązań analitycznych.
Many processes in natural and social sciences can be modeled by systems of interacting objects. It is usually very difficult to obtain analytic expressions describing time evolution and equilibrium behavior of such systems. Very often we rely only on computer simulations. Fortunately, in many cases one can construct useful approximation schemes and derive exact results which capture some specific features of a given process. A frequent approach is to replace interactions between objects by a mean interaction. Here we illustrate a self-consistent mean-field approximation in two examples: the Ising model of interacting spins and a simple model of a self-regulating gene.
Źródło:
Mathematica Applicanda; 2012, 40, 1
1730-2668
2299-4009
Pojawia się w:
Mathematica Applicanda
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Genetic study of familial cases of Alzheimers disease.
Autorzy:
Kowalska, Anna
Pruchnik-Wolińska, Danuta
Florczak, Jolanta
Modestowicz, Renata
Szczech, Józef
Kozubski, Wojciech
Rossa, Grzegorz
Wender, Mieczysław
Powiązania:
https://bibliotekanauki.pl/articles/1043353.pdf
Data publikacji:
2004
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
neurodegeneration
dementia
presenilin 1 gene
amyloid precursor protein gene
Alzheimer's disease
mutation
presenilin 2 gene
Opis:
A small number (1-5%) of Alzheimer's disease (AD) cases associated with the early-onset form of the disease (EOAD) appears to be transmitted as a pure genetic, autosomal dominant trait. To date, three genes responsible for familial EOAD have been identified in the human genome: amyloid precursor protein (APP), presenilin 1 (PS1), and presenilin 2 (PS2). Mutations in these genes account for a significant fraction (18 to 50%) of familial cases of early onset AD. The mutations affect APP processing causing increased production of the toxic Aβ42 peptide. According to the "amyloid cascade hypothesis", aggregation of the Aβ42 peptide in brain is a primary event in AD pathogenesis. In our study of twenty AD patients with a positive family history of dementia, 15% (3 of 20) of the cases could be explained by coding sequence mutations in the PS1 gene. Although a frequency of PS1 mutations is less than 2% in the whole population of AD patients, their detection has a significant diagnostic value for both genetic counseling and treatment in families with AD.
Źródło:
Acta Biochimica Polonica; 2004, 51, 1; 245-242
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Gene pool variation of Fragaria × ananassa (Duch.) and Fragaria vesca (L.)
Zmienność w obrębie zasobów genowych Fragaria × ananassa (Duch.) i Fragaria vesca (L.)
Autorzy:
Dziadczyk, E.
Dziadczyk, P.
Tyrka, M.
Powiązania:
https://bibliotekanauki.pl/articles/11543047.pdf
Data publikacji:
2015
Wydawca:
Uniwersytet Przyrodniczy w Lublinie. Wydawnictwo Uniwersytetu Przyrodniczego w Lublinie
Tematy:
gene variant
Fragaria ananassa
Fragaria vesca
gene pool
hybrid
interspecific hybrid
Opis:
Gene pool variation of twenty varieties and breeding clones of Fragaria × ananassa, nine varieties and breeding lines of Fragaria vesca, and one new interspecific hybrid designated Fragaria × anavesca was analysed with three DNA marker systems. ISSR reactions with four primers produced 45 polymorphic markers. Similarly, RAPD analyses with three primers produced 26 markers and SSR method with three primer pairs revealed 28 different alleles. The total number of 99 polymorphic markers allowed distinguishing clearly a group of F. × ananassa genotypes from that of F. vesca genotypes with F. × anavesca in between of these two. RAPD markers proved to be more informative than ISSRs as 3 of 26 were specific to F. × ananassa only and one exclusively to F. vesca and F. × anavesca. Thus, the presumed hybrid nature of F. × anavesca was effectively confirmed by RAPD markers. Especially important was the 1100bp long PCR product of the B104 primer present in all F. vesca genotypes as well as in F. × anavesca but absent in F. × ananassa. Presence of F. vesca DNA in the hybrid F. × anavesca was additionally corroborated by the 223bp product of the UDF017 primer pair and the 185bp-long band generated with the UDF006 primer pair.
Analizowano zmienność w puli genów dwudziestu odmian i klonów hodowlanych Fragaria × ananassa, dziewięciu odmian i linii hodowlanych Fragaria vesca i nowego prawdopodobnego mieszańca międzygatunkowego nazwanego Fragaria × anavesca przy zastosowaniu trzech systemów markerów DNA. Cztery startery ISSR wygenerowały 45 markerów polimorficznych. Trzy startery RAPD dały 26 markerów, natomiast trzy startery SSR – 28 markerów polimorficznych. 99 uzyskanych markerów polimorficznych pozwoliło na jednoznaczne odróżnienie grupy genotypów należących do gatunku F. × ananassa od grupy genotypów należących do gatunku F. vesca z mieszańcem F. × anavesca sytuującym się pomiędzy nimi. Markery RAPD okazały się lepszym źródłem informacji niż ISSR, ponieważ 3 spośród 26 były specyficzne tylko dla F. × ananassa, a jeden wyłącznie dla F. vesca i F. × anavesca. W ten sposób, za pomocą markerów RAPD, potwierdzona została przypuszczalna wcześniej mieszańcowość F. × anavesca. Szczególnie istotny okazał się produkt reakcji PCR ze starterem B104 o długości 1100pz obecny we wszystkich genotypach F. vesca oraz F. × anavesca, a niewystępujący u F. × ananassa. Obecność DNA pochodzącego z F. vesca w mieszańcu F. × anavesca została dodatkowo potwierdzona poprzez produkt pary starterów UDF017 o długości 223pz i produkt pary starterów UDF006 o długości 185pz.
Źródło:
Acta Scientiarum Polonorum. Hortorum Cultus; 2015, 14, 2; 41-50
1644-0692
Pojawia się w:
Acta Scientiarum Polonorum. Hortorum Cultus
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Carrier-state of D allele in ACE gene insertion/deletion polymorphism is associated with coronary artery disease, in contrast to the C677→T transition in the MTHFR gene.
Autorzy:
Żak, Iwona
Niemiec, Paweł
Sarecka, Beata
Balcerzyk, Anna
Ciemniewski, Zbigniew
Rudowska, Ewa
Dyląg, Stanisław
Powiązania:
https://bibliotekanauki.pl/articles/1043632.pdf
Data publikacji:
2003
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
angiotensin converting enzyme gene (ACE)
coronary artery disease
methylenetetrahydrofolate reductase gene (MTHFR)
polymorphisms
Opis:
Angiotensin I-converting enzyme (ACE), which plays an important role in blood pressure regulation, and methylenetetrahydrofolate reductase (MTHFR) involved in homocysteine metabolism belong to a large group of polypeptides which may be potential risk factors for atherosclerosis and coronary artery disease (CAD). To assess whether polymorphisms of the genes encoding these peptides are associated with CAD in Silesian we conducted a study among 68 individuals suffering from CAD (including 52 cases after myocardial infarction), 51 subjects with positive family history of CAD and 111 controls. We analysed the distribution of genotypes and allele frequencies of the insertion/deletion (I/D) polymorphism in the ACE gene using PCR amplification, and the C677→T polymorphism in the MTHFR gene using PCR-RFLP analysis. We found that D allele frequency was significantly higher in CAD patients (61%) than in controls (43%) (P = 0.001, OR = 2.06). The D allele carriers (DD + ID genotypes) were more frequent in the CAD patients (85%) compared to control group (65%) (P = 0.003, OR = 3.14), whereas the familial CAD risk group shows the highest frequency of the ID genotype (57% vs 43% in controls). In contrast, the MTHFR polymorphism does not seem to be associated with the disease. Our data indicate that in Silesian CAD patients the disease is strongly associated with carrier-state of the ACE D allele, but not with the C677→T transition in the MTHFR gene.
Źródło:
Acta Biochimica Polonica; 2003, 50, 2; 527-534
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Wyświetlanie 1-50 z 1094

Ta witryna wykorzystuje pliki cookies do przechowywania informacji na Twoim komputerze. Pliki cookies stosujemy w celu świadczenia usług na najwyższym poziomie, w tym w sposób dostosowany do indywidualnych potrzeb. Korzystanie z witryny bez zmiany ustawień dotyczących cookies oznacza, że będą one zamieszczane w Twoim komputerze. W każdym momencie możesz dokonać zmiany ustawień dotyczących cookies