Molecular typing of Staphylococcus aureus based on PCR-RFLP of coa gene and RAPD analysis

Szczegóły
Opis

Tytuł:: Molecular typing of Staphylococcus aureus based on PCR-RFLP of coa gene and RAPD analysis
Autorzy:: Karakulska, J.
Pobucewicz, A.
Nawrotek, P.
Muszynska, M.
Furowicz, A.J.
Czernomysy-Furowicz, D.
Powiązania:: https://bibliotekanauki.pl/articles/31963.pdf
Data publikacji:: 2011
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: molecular typing
Staphylococcus aureus
PCR-RFLP method
coa gene
random amplified polymorphic DNA analysis
mastitis
nuc gene
gap gene
milk sample
species identification
Źródło:: Polish Journal of Veterinary Sciences; 2011, 14, 2
1505-1773
Język:: angielski
Prawa:: CC BY-NC-ND: Creative Commons Uznanie autorstwa - Użycie niekomercyjne - Bez utworów zależnych 3.0 PL
Dostawca treści:: Biblioteka Nauki
: Artykuł

Przejdź do źródła

The aim of this study was molecular identification of S. aureus strains isolated from mastitic milk samples and establishing the genetic relationship between strains isolated from cows belonging to the same herd. In all 43 isolated strains the gap gene (930 bp) was amplified, which enabled their affiliation to the Staphylococcus genus to be established. PCR-RFLP with AluI endonuclease of the gap gene as well as nuc (450 bp) and coa (1130 bp) gene amplification allowed precise S. aureus species identification. One hundred percent of the genetic relationship between strains was established via RAPD-PCR and coa-typing.

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