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Tytuł:
Ship Route Design for Avoiding Heavy Weather and Sea Conditions
Autorzy:
Cai, Y.
Wen, Y.
Wu, L.
Powiązania:
https://bibliotekanauki.pl/articles/116290.pdf
Data publikacji:
2014
Wydawca:
Uniwersytet Morski w Gdyni. Wydział Nawigacyjny
Tematy:
Ship Route
Ship Route Design
green energy policy
Heavy Weather
Avoiding Heavy Weather
Avoiding Sea Conditions
Baltic Sea
Gulf of Finland
Opis:
This paper covers the current state of maritime oil transportation in the Baltic Sea and the development of oil transportation in the 2000s, as well as estimations of transported oil volumes in 2020 and 2030 in the Gulf of Finland. The scenarios were formulated on the basis of a current state analysis, energy and transportation strategies and scenarios and expert assessments. The study showed that the volumes of oil transportation in the Gulf of Finland will increase only moderately compared to the current status: 9.5-33.8 %, depending on the scenario. Green energy policy favours renewable energy sources, which can be seen in the smaller volumes of transported oil in the 2030 scenarios compared to the 2020 scenarios. In the Slow development 2020 scenario, oil transport volumes for 2020 are expected to be 170.6 Mt (million tonnes), in the Average development 2020 187.1 Mt and in the Strong development 2020 201.5 Mt. The corresponding oil volumes for the 2030 scenarios were 165 Mt for the Stagnating development 2030 scenario, 177.5 Mt for the Towards a greener society 2030 scenario and 169.5 Mt in the Decarbonising society 2030 scenario.
Źródło:
TransNav : International Journal on Marine Navigation and Safety of Sea Transportation; 2014, 8 no. 4; 551-556
2083-6473
2083-6481
Pojawia się w:
TransNav : International Journal on Marine Navigation and Safety of Sea Transportation
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The ascorbate metabolism as a target for the breeding of stress resistant rice
Autorzy:
Frei, M.
Holler, S.
Ueda, Y.
Wu, L.
Powiązania:
https://bibliotekanauki.pl/articles/80002.pdf
Data publikacji:
2013
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
conference
ascorbate
abiotic stress
plant tissue
oxidative stress
rice
stress resistance
zinc deficiency
tropospheric ozone
iron toxicity
Źródło:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2013, 94, 2
0860-7796
Pojawia się w:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Stochastic fractal based multiobjective fruit fly optimization
Autorzy:
Zuo, C.
Wu, L.
Zeng, Z. F.
Wei, H. L.
Powiązania:
https://bibliotekanauki.pl/articles/330026.pdf
Data publikacji:
2017
Wydawca:
Uniwersytet Zielonogórski. Oficyna Wydawnicza
Tematy:
multiobjective optimization
fruit fly optimization algorithm
stochastic fractal
optymalizacja wielokryterialna
algorytm optymalizacji
fraktal stochastyczny
Opis:
The fruit fly optimization algorithm (FOA) is a global optimization algorithm inspired by the foraging behavior of a fruit fly swarm. In this study, a novel stochastic fractal model based fruit fly optimization algorithm is proposed for multiobjective optimization. A food source generating method based on a stochastic fractal with an adaptive parameter updating strategy is introduced to improve the convergence performance of the fruit fly optimization algorithm. To deal with multiobjective optimization problems, the Pareto domination concept is integrated into the selection process of fruit fly optimization and a novel multiobjective fruit fly optimization algorithm is then developed. Similarly to most of other multiobjective evolutionary algorithms (MOEAs), an external elitist archive is utilized to preserve the nondominated solutions found so far during the evolution, and a normalized nearest neighbor distance based density estimation strategy is adopted to keep the diversity of the external elitist archive. Eighteen benchmarks are used to test the performance of the stochastic fractal based multiobjective fruit fly optimization algorithm (SFMOFOA). Numerical results show that the SFMOFOA is able to well converge to the Pareto fronts of the test benchmarks with good distributions. Compared with four state-of-the-art methods, namely, the non-dominated sorting generic algorithm (NSGA-II), the strength Pareto evolutionary algorithm (SPEA2), multi-objective particle swarm optimization (MOPSO), and multiobjective self-adaptive differential evolution (MOSADE), the proposed SFMOFOA has better or competitive multiobjective optimization performance.
Źródło:
International Journal of Applied Mathematics and Computer Science; 2017, 27, 2; 417-433
1641-876X
2083-8492
Pojawia się w:
International Journal of Applied Mathematics and Computer Science
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Effects of porcine epidemic diarrhea virus infection on tight junction protein gene expression and morphology of the intestinal mucosa in pigs
Autorzy:
Zong, Q.F.
Huang, Y.J.
Wu, L.S.
Wu, Z.C.
Wu, S.L.
Bao, W.B.
Powiązania:
https://bibliotekanauki.pl/articles/2087524.pdf
Data publikacji:
2019
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
pig
tight junction protein
PEDV
diarrhea
Źródło:
Polish Journal of Veterinary Sciences; 2019, 2; 345-353
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Effect of gas holdup on the efficiency of cyclonic-static microbubble flotation column for oily wastewater treatment
Autorzy:
Ning, K.
Huang, G.
Xu, H.
Wu, L.
Li, X.
Wang, Y.
Powiązania:
https://bibliotekanauki.pl/articles/208139.pdf
Data publikacji:
2018
Wydawca:
Politechnika Wrocławska. Oficyna Wydawnicza Politechniki Wrocławskiej
Tematy:
bubble columns
efficiency
flotation
wastewater treatment
oil water separation
kolumna flotacyjna
efektywność
flotacja
oczyszczanie ścieków
separacja wody
Opis:
A cyclonic-static microbubble flotation column of a novel construction was used in oil-water separation fields and has high efficiency for oil-water separation. The gas holdup is a key parameter for the evaluation of the performance of a flotation column. The gas holdup, closely related to the bubble size, bubble velocity and superficial gas velocity, is one of the most important parameters characterizing the hydrodynamics of a bubble column. The effect of gas holdup in a cyclonic-static microbubble flotation column was investigated. In addition, several operating parameters such as the circulating pressure, superficial gas velocity, and frother consumption were also investigated. The gas holdup was positively correlated to the superficial gas velocity. The gas holdup of clean water and oil wastewater increased along with the increase of the frother consumption. The separation mechanism of cyclonic-static microbubble flotation column was analyzed.
Źródło:
Environment Protection Engineering; 2018, 44, 2; 5-17
0324-8828
Pojawia się w:
Environment Protection Engineering
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
A fault diagnosis method for substation grounding grid based on the square-wave frequency domain model
Autorzy:
Zhang, P.-H.
He, J.-J.
Zhang, D.-D.
Wu, L.-M.
Powiązania:
https://bibliotekanauki.pl/articles/221635.pdf
Data publikacji:
2012
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
square-wave
frequency characteristics
grounding grid
fault diagnosis
Opis:
Current methods of fault diagnosis for the grounding grid using DC or AC are limited in accuracy and cannot be used to identify the locations of the faults. In this study, a new method of fault diagnosis for substation grounding grids is proposed using a square-wave. A frequency model of the grounding system is constructed by analyzing the frequency characteristics of the soil and the grounding conductors into which two different frequency square-wave sources are injected. By analyzing and comparing the corresponding information of the surface potentials of the output signals, the faults of the grounding grid can be diagnosed and located. Our method is verified by software simulation, scale model experiments and field experiments.
Źródło:
Metrology and Measurement Systems; 2012, 19, 1; 63-71
0860-8229
Pojawia się w:
Metrology and Measurement Systems
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Crown and root biomass equations for the small trees of Pinus koraiensis under canopy
Autorzy:
Wang, J.
Fan, J.
Fan, X.
Zhang, Ch.
Wu, L.
v.Gadow, K.
Powiązania:
https://bibliotekanauki.pl/articles/41047.pdf
Data publikacji:
2013
Wydawca:
Polska Akademia Nauk. Instytut Dendrologii PAN
Opis:
Estimation of tree biomass is an essential part of studies on carbon sequestration and cycling in forest ecosystem. Small trees grow in the understory and allometric development is different from that of mature trees. However, less attention has been paidto biomass estimates of small trees, especially in mixedforest where tree competition is intensive. Tree allometric equations at both branch level andat whole tree level were, thus, developed and compared for the small trees of Korean pine (Pinus koraiensis) in a mixedstandin northeastern China. At branch level, the best model for live branch biomass was one which used a combination of branch diameter, branch length, whorl position and relative branch depth. For needle biomass, the best model did not significantly improve the estimate with more variables. At whole tree level, stem diameter at breast height (DBH) was a significant determinant of biomass for different components. Tree height did not significantly improve biomass estimation at all. Tree crown variables provedto be useful for estimating all biomass components except the fine roots. The variable measuring abovegroundcompetition intensity was a significant negative determinant of biomass components except canopy biomass. Comparisons to published equations for the same species growing in Heilongjiang province in northeastern China andin central South Korea, were also presented. Both total aboveground biomass and belowground biomass in our study showed somewhat smaller values for a given diameter than the trees growing in other two places.
Źródło:
Dendrobiology; 2013, 70
1641-1307
Pojawia się w:
Dendrobiology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Oxygen-containing Tetrazole Salts of 3-Hydrazino-4-amino-1,2,4-triazole (HATr): Nitrogen-rich Ionic Materials with High Thermal Stability
Autorzy:
Wu, J.-T.
Zhang, J.-G.
Yin, X.
Li, T.
Wu, L.
Zhang, Z.-B.
Powiązania:
https://bibliotekanauki.pl/articles/358927.pdf
Data publikacji:
2017
Wydawca:
Sieć Badawcza Łukasiewicz - Instytut Przemysłu Organicznego
Tematy:
energetic salts
thermal stability
oxygen-containing tetrazoles
3-hydrazino-4-amino-1,2,4-triazole
Opis:
Energetic salts of doubly-protonated 3-hydrazino-4-amino-1,2,4-triazole (2-4), and mono-protonated 3-hydrazino-4-amino-1,2,4-triazole (5-7), have been prepared in high yields from the corresponding oxygen-containing tetrazoles by (i) reaction of free acids and neutral 3-hydrazino-4-amino-1,2,4-triazole, or (ii) metathesis reactions of 3-hydrazinium-4-amino-1H-1,2,4-triazolium di-chloride with the silver salts of the corresponding oxygen-containing tetrazoles. All of these energetic salts were fully characterized by single-crystal X-ray diffraction, FT-IR and DSC measurements. All of the structures are dominated by extensive hydrogen bonds due to amino groups, hydrazino groups and oxygen atoms in the molecules. These salts exhibit good thermal stability, especially the salt 3-hydrazinium-4-amino-1H-1,2,4-triazolium 1H,1’H-5,5’-bitetrazole-1,1’-diolate (4), which exhibits a measured onset of decomposition temperature of 237 °C. Quantum chemical calculations, carried out using the Gaussian 03 suite of programs and based on the experimental densities were used to calculate the detonation pressures and velocities of all of the salts.
Źródło:
Central European Journal of Energetic Materials; 2017, 14, 1; 217-232
1733-7178
Pojawia się w:
Central European Journal of Energetic Materials
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
An EL-model based passivity control of four-phase interleaved PFC
Autorzy:
Hua-Wu, L.
Hong-Xing, M.
Jian-Feng, J.
Xi-Jun, Y.
Xing-Hua, Y.
Powiązania:
https://bibliotekanauki.pl/articles/140472.pdf
Data publikacji:
2013
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
four-phase interleaved PFC
passivity based control
damping injection
current sparing
Opis:
With the continuous increase of output power ratings, multi-phase (multichannel) interleaved power factor corrector (IPFC) is gradually employed in domestic and commercial inverter air-conditioners. IPFC can solve several main problems, such as power rating increase, power device selection, input current ripple reduction as well as inductor on-board mounting. But for a multi-phase IPFC, the key problem is that it should show rapid dynamic responds and good current sharing capability, so in this paper the aim is to improve the dynamic performance and current sharing capability by means of passivity control theory. Considering the power circuit topology of a four-phase IPFC, an EL (Euler-Lagrange) mathematical model is established when the IPFC operates in continuous conduction mode (CCM). Then the passivity of the four-phase IPFC is proved, and the passivity-based controller using the state variables feedback and damping injection method is designed. The proposed control scheme, which is easy to control and needs no proportion integral controller, has strong robustness on disturbance from singlephase AC input voltage, the load as well as the parameters of the employed devices. Even in wide-range load condition, the mains current has a fast dynamic response and the average output voltage almost keep unchanged. As a result, the main functions of the four-phase IPFC are implemented including nearly unitary power factor and constant DC output voltage. Meanwhile, the four-phase IPFC acquires an excellent current sparing effect after using passivity-based controller. The above analysis has been proved with simulated results by means of MATLAB/SIMULINK and experimental results, showing that the passivity-based IPFC controller has superior performances and feasibility.
Źródło:
Archives of Electrical Engineering; 2013, 62, 4; 613-628
1427-4221
2300-2506
Pojawia się w:
Archives of Electrical Engineering
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Improved fuzzy feedback linearization and Sinswat-transformation control of inverted pendulum
Autorzy:
Chien, T. L.
Chen, C. C.
Chen, Y. C.
Wu, S. L.
Powiązania:
https://bibliotekanauki.pl/articles/970222.pdf
Data publikacji:
2010
Wydawca:
Polska Akademia Nauk. Instytut Badań Systemowych PAN
Tematy:
fuzzy logic control
almost disturbance decoupling
feedback linearization approach
composite Lyapunov approach
inverted pendulum control system
Opis:
This paper studies the output tracking and almost disturbance decoupling problem of nonlinear control systems with uncertainties via fuzzy logic control and feedback linearization approach. The main contribution of this study is to construct a controller, under appropriate conditions, such that the resulting closed-loop system enjoys for any initial condition and bounded tracking signal the following characteristics: input-to-state stability with respect to disturbance inputs and almost disturbance decoupling, i.e., the influence of disturbances on the L2 norm of the output tracking error can be arbitrarily attenuated by increasing some adjustable parameters. The underlying theoretical approaches are the differential geometry approach and the composite Lyapunov approach. One example, which cannot be solved by the approach from the first paper (Marino et al., 1989) on the almost disturbance decoupling problem, is proposed in this paper to exploit the fact that the almost disturbance decoupling and the convergence rate performances are easily achieved by virtue of our approach. In order to demonstrate the practical applicability, the paper takes up the study of an inverted pendulum control system.
Źródło:
Control and Cybernetics; 2010, 39, 4; 1069-1093
0324-8569
Pojawia się w:
Control and Cybernetics
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Quantitative processing of situation awareness for autonomous ships navigation
Autorzy:
Zhou, X. Y.
Liu, Z. J.
Wu, Z. L.
Wang, F. W.
Powiązania:
https://bibliotekanauki.pl/articles/117424.pdf
Data publikacji:
2019
Wydawca:
Uniwersytet Morski w Gdyni. Wydział Nawigacyjny
Tematy:
autonomous vessel
autonomous ship
autonomous ships navigation
situation awareness (SA)
quantitative processing
navigation safety
remotely controlled vessel
maritime unmanned navigation through intelligence in networks (MUNIN)
Opis:
The first ever attempt at fully autonomous dock-to-dock operation has been tested and demonstrated successfully at the end of 2018. The revolutionary shift is feared to have a negative impact on the safety of navigation and the getting of real-time situation awareness. Especially, the centralized context onboard could be changed to a distributed context. In navigation safety domain, monitoring, control, assessment of dangerous situations, support of operators of decision-making support system should be implemented in real time. In the context of autonomous ships, decision-making processes will play an important role under such ocean autonomy, therefore the same technologies should consist of adequate system intelligence. At the same time, situation awareness is the key element of the decision-making processes. Although there is substantial research on situation awareness measurement techniques, they are not suitable to directly execute quantitative processing for the situation awareness of autonomous ships navigation. Hence, a novel quantitative model of situation awareness is firstly proposed based on the system safety control structure of remotely controlled vessel. The data source is greatly limited, but the main result still indicates that the probability of operator lose adequate situation awareness of the autonomous ship is significantly higher than the conventional ship. Finally, the paper provides a probabilistic theory and model for high-level abstractions of situation awareness to guide future evaluation of the navigation safety of autonomous ships.
Źródło:
TransNav : International Journal on Marine Navigation and Safety of Sea Transportation; 2019, 13, 1; 25-31
2083-6473
2083-6481
Pojawia się w:
TransNav : International Journal on Marine Navigation and Safety of Sea Transportation
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Hydrogen sulfide modulates gastric acid secretion in rats via involvement of substance P and nuclear factor-kappaB signaling
Autorzy:
Sun, H.-Z.
Gong, X.-Y.
Wu, L.
Wang, X.-X.
Nie, Y.-N.
Shang, R.
Wang, H.
Li, Y.-C.
Sun, Q.-F.
Gao, P.-F.
Bi, J.-X.
Powiązania:
https://bibliotekanauki.pl/articles/69523.pdf
Data publikacji:
2018
Wydawca:
Polskie Towarzystwo Fizjologiczne
Tematy:
hydrogen sulphide
gastric acid secretion
rat
nuclear factor-kappaB
substance P
transient receptor potential vanilloid type 1
neurokinin-1
Źródło:
Journal of Physiology and Pharmacology; 2018, 69, 3
0867-5910
Pojawia się w:
Journal of Physiology and Pharmacology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Multivariate statistical analysis of water quality and phytoplankton characteristics in Daya Bay, China, from 1999 to 2002
Autorzy:
Wang, Y.S.
Lou, Z.P.
Sun, C.C.
Wu, M.L.
Han, S.H.
Powiązania:
https://bibliotekanauki.pl/articles/48049.pdf
Data publikacji:
2006
Wydawca:
Polska Akademia Nauk. Instytut Oceanologii PAN
Tematy:
South China Sea
water quality
China
phytoplankton
Daya Bay
multivariate statistical analysis
Opis:
This study analyzed seasonal physicochemical and phytoplankton data collected at 12 marine monitoring stations in Daya Bay from 1999 to 2002. Cluster analysis based on water quality and phytoplankton parameters measured at the 12 stations could be grouped into three clusters: cluster I – stations S1,S2 , S7 and S11 in the southern part and the north-eastern part of Daya Bay; cluster II – stations S5, S6,S9 ,S1 0 and S12 in the central and north-eastern parts of Daya Bay; cluster III – stations S3,S 4 and S8 in the cage culture areas in the south-western part of Daya Bay and in the north-western part of the Bay near Aotou harbor. Bivariate correlations between phytoplankton density and the major physical and nutrient factors were calculated for all stations. Factor analysis shows that there were high positive loadings of pH,T IN and the ratio of TIN to PO4-P in the three clusters, which indicates that all the stations in the three clusters were primarily grouped according to their respective nutrient conditions.
Źródło:
Oceanologia; 2006, 48, 2
0078-3234
Pojawia się w:
Oceanologia
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Effects of porcine epidemic diarrhea virus infection on Toll-like receptor expression and cytokine levels in porcine intestinal epithelial cells
Autorzy:
Wang, F.
Wang, S.Q.
Wang, H.F.
Wu, Z.C.
Bao, W.B.
Wu, S.L.
Powiązania:
https://bibliotekanauki.pl/articles/2087351.pdf
Data publikacji:
2020
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
pig
porcine epidemic diarrhea virus
TLRs
innate immunity
Źródło:
Polish Journal of Veterinary Sciences; 2020, 23, 1; 119-126
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Effect of bamboo vinegar powder on the expression of the immune-related genes MyD88 and CD14 in weaning piglets
Autorzy:
Qin, W.Y.
Gan, L.N.
Dong, L.
Yu, L.H.
Wu, S.L.
Bao, W.B.
Powiązania:
https://bibliotekanauki.pl/articles/2087674.pdf
Data publikacji:
2018
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
bamboo vinegar powder
pig
antibiotics
MyD88 gene
CD14 gene
Źródło:
Polish Journal of Veterinary Sciences; 2018, 21, 3; 525-532
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Using chemometrics to identify water quality in Daya Bay, China
Autorzy:
Wu, M.-L.
Wang, Y.-S.
Sun, C.-C.
Wang, H.
Lou, Z.-P.
Dong, J.-D.
Powiązania:
https://bibliotekanauki.pl/articles/49096.pdf
Data publikacji:
2009
Wydawca:
Polska Akademia Nauk. Instytut Oceanologii PAN
Tematy:
robust principal component analysis
water quality
chemometrics
China
cluster analysis
Daya Bay
Opis:
In this paper, chemometric approaches based on cluster analysis, classical and robust principal component analysis were employed to identify water quality in Daya Bay (DYB), China. The results show that these approaches divided water quality in DYB into two groups: stations S3, S8, S10 and S11 belong to cluster A, which lie in Dapeng Cove, Aotou Harbor and the north-eastern part of DYB, where water quality is related mainly to anthropogenic activities. The other stations belong to cluster B, which lie in the southern, central and eastern parts of DYB, where the quality is related mainly to water exchange with the South China Sea. Cluster analysis yields good results as a first exploratory method for evaluating spatial difference, but it fails to demonstrate the relationship between variables and environmental quality on the one hand and the untreated data on the other. However, with the aid of suitable chemometric approaches, the relationship between samples or variables can be investigated. Classical and robust principal component analysis can provide a visual aid for identifying the water environment in DYB, and then extracting specific information about relationships between variables and spatial variation trends in water quality.
Źródło:
Oceanologia; 2009, 51, 2; 217-232
0078-3234
Pojawia się w:
Oceanologia
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
LBP gene methylation involved in mRNA expression and resistance to E. coli F18 in weaned piglets
Autorzy:
Gan, L.N.
Bao, W.B.
Wu, S.L.
Qin, W.Y.
Sun, L.
Zhao, C.X.
Powiązania:
https://bibliotekanauki.pl/articles/2087872.pdf
Data publikacji:
2017
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
LBP gene
methylation analysis of the LBP region
E.coli F18 strain
weaned piglets
Źródło:
Polish Journal of Veterinary Sciences; 2017, 4; 643-650
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Use of fluorescence quantitative polymerase chain reaction (PCR) for the detection of Escherichia coli adhesion to pig intestinal epithelial cells
Autorzy:
Dai, C.H.
Gan, L.N.
Qin, W.U.
Zi, C.
Zhu, G.Q.
Wu, S.L.
Bao, W.B.
Powiązania:
https://bibliotekanauki.pl/articles/31510.pdf
Data publikacji:
2016
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
An efficient and accurate method to test Escherichia coli (E. coli) adhesion to intestinal epithelial cells will contribute to the study of bacterial pathogenesis and the function of genes that encode receptors related to adhesion. This study used the quantitative real-time polymerase chain reaction (qPCR) method. qPCR primers were designed from the PILIN gene of E. coli F18ab, F18ac, and K88ac, and the pig β-ACTIN gene. Total deoxyribonucleic acid (DNA) from E. coli and intestinal epithelial cells (IPEC-J2 cells) were used as templates for qPCR. The 2−ΔΔCt formula was used to calculate the relative number of bacteria in cultures of different areas. We found that the relative numbers of F18ab, F18ac, and K88ac that adhered to IPEC-J2 cells did not differ significantly in 6-, 12-, and 24-well culture plates. This finding indicated that there was no relationship between the relative adhesion number of E. coli and the area of cells, so the method of qPCR could accurately test the relative number of E. coli. This study provided a convenient and reliable testing method for experiments involving E. coli adhesion, and also provided innovative ideas for similar detection methods.
Źródło:
Polish Journal of Veterinary Sciences; 2016, 19, 3
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Differential expression of Toll - like receptor 4 signaling pathway genes in Escherichia coli F18 - resistant and - sensitive Meishan piglets
Autorzy:
Liu, Y.
Gan, L.N.
Qin, W.Y.
Sun, S.Y.
Zhu, G.Q.
Wu, S.L.
Bao, W.B.
Powiązania:
https://bibliotekanauki.pl/articles/31946.pdf
Data publikacji:
2016
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
The Toll-like receptor 4 (TLR4) signaling pathway is an important inflammatory pathways associated with the progression of numerous diseases. The aim of the present study was to investigate the relationship between TLR4 signaling and resistance to Escherichia coli F18 in locally weaned Meishan piglets. Using a real-time PCR approach, expression profiles were determined for key TLR4 signaling pathway genes TLR4, MyD88, CD14, IFN-α, IL-1β and TNF-α in the spleen, thymus, lymph nodes, duodenum and jejunum of E. coli F18-resistant and -sensitive animals. TLR4 signaling pathway genes were expressed in all the immune organs and intestinal tissues, and the expression was generally higher in the spleen and lymph nodes. TLR4 transcription was higher in the spleen of sensitive piglets (p<0.05), but there was no significant difference in TLR4 mRNA levels in other tissues. Similarly, CD14 transcription was higher in lymph nodes of sensitive animals (p<0.05) but not in other tissues. IL-1β expression was higher in the spleen and in the duodenum of resistant piglets (p<0.05, p<0.01, respectively), and there were no significant differences in other tissues. There were also no significant differences in the expression of MyD88, TNF-α and IFN-α between sensitive and resistant piglets (p>0.05). These results further confirm the involvement of the TLR4 signaling pathway in resistance to E. coli F18 in Meishan weaned piglets. The resistance appeared to be mediated via downregulation of TLR4 and CD14, and upregulation of MyD88 that may promote the release of cytokines TNF-α, IL-1β, IFN-α and other inflammatory mediators which help to fight against E. coli F18 infection.
Źródło:
Polish Journal of Veterinary Sciences; 2016, 19, 2
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Demonstration of a new indicator for studying upwelling in the Northern South China Sea
Autorzy:
Lin, L.
Wang, Y.-S.
Sun, C.-C.
Li, N.
Wang, H.
Mitchell, B.G.
Wu, M.-L.
Song, H.
Wu, J.-F.
Powiązania:
https://bibliotekanauki.pl/articles/48889.pdf
Data publikacji:
2011
Wydawca:
Polska Akademia Nauk. Instytut Oceanologii PAN
Tematy:
China Sea
cluster analysis
multivariate statistical analysis
principal component analysis
remote sensing
satellite monitoring
sea surface temperature
silicate
spatial distribution
upwelling
Opis:
In order to demonstrate that silicate (SiO3-Si) can be used as an indicator to study upwelling in the northern South China Sea, hierarchical cluster analysis (CA) and principle component analysis (PCA) were applied to analyse the metrics of the data consisting of 14 physical-chemical-biological parameters at 32 stations. CA categorized the 32 stations into two groups (low and high nutrient groups). PCA was applied to identify five Principal Components (PCs) explaining 78.65% of the total variance of the original data. PCA found important factors that can describe nutrient sources in estuarine, upwelling, and non-upwelling areas. PC4, representing the upwelling source, is strongly correlated to SiO3-Si. The spatial distribution of silicate from the surface to 200 m depth clearly showed the upwelling regions, which is also supported by satellite observations of sea surface temperature.
Źródło:
Oceanologia; 2011, 53, 2
0078-3234
Pojawia się w:
Oceanologia
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
A counterexample in comonotone approximation in $L^p$ space
Autorzy:
Wu, Xiang
Zhou, Song
Powiązania:
https://bibliotekanauki.pl/articles/1396162.pdf
Data publikacji:
1993
Wydawca:
Polska Akademia Nauk. Instytut Matematyczny PAN
Opis:
Refining the idea used in [24] and employing very careful computation, the present paper shows that for 0 < p ≤ ∞ and k ≥ 1, there exists a function $f ∈ C_{[-1,1]}^k$, with $f^{(k)}(x)≥ 0$ for x ∈ [0,1] and $f^{(k)}(x) ≤ 0$ for x ∈ [-1,0], such that lim sup_{n→∞} (e_n^{(k)}(f)_p) / (ω_{k+2+[1/p]}(f,n^{-1})_{p}) = + ∞ where $e_n^{(k)}(f)_p$ is the best approximation of degree n to f in $L^p$ by polynomials which are comonotone with f, that is, polynomials P so that $P^{(k)}(x)f^{(k)}(x) ≥ 0$ for all x ∈ [-1,1]. This theorem, which is a particular case of a more general one, gives a complete solution to the converse result in comonotone approximation in $L^p$ space for 1 < p ≤ ∞.
Źródło:
Colloquium Mathematicum; 1993, 64, 2; 265-274
0010-1354
Pojawia się w:
Colloquium Mathematicum
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Energetic Nitrogen-rich Salts
Autorzy:
Wu, J.-W.
Zhang, J.-G.
Zhang, T.-L.
Yang, L.
Powiązania:
https://bibliotekanauki.pl/articles/358479.pdf
Data publikacji:
2015
Wydawca:
Sieć Badawcza Łukasiewicz - Instytut Przemysłu Organicznego
Tematy:
energetic salts
synthesis
characterization
crystal structure
review
Opis:
Energetic salts as a unique class of energetic materials have recently been investigated widely, since they possess advantages over their atomically similar nonionic analogues – they tend to exhibit lower vapor pressures, higher densities and better thermal stabilities. Furthermore, these salts are readily improved upon by the appropriate combination of different cations and anions. Over the last twenty years, our group has continued to synthesize and study novel ionic energetic materials. Here, we have summarized all of the series of nitrogen-rich energetic salts. This review gives an overview of the various studies dealing with synthetic aspects and some of the physicochemical properties of ionic compounds that are based on ammonia, guanidine, semicarbazide, carbohydrazide, tetrazine, tetrazole, triazole and imidazole. In addition, their potential applications in the fields of explosives and propellants are discussed. We hope these investigations will be helpful in providing a wider insight for future research in the area of energetic salts.
Źródło:
Central European Journal of Energetic Materials; 2015, 12, 3; 417-437
1733-7178
Pojawia się w:
Central European Journal of Energetic Materials
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Thermal effect on damaged stay-cables
Autorzy:
Wang, L.
Wu, Y.
Wang, D.
Powiązania:
https://bibliotekanauki.pl/articles/280543.pdf
Data publikacji:
2014
Wydawca:
Polskie Towarzystwo Mechaniki Teoretycznej i Stosowanej
Tematy:
stay cables
damage
thermal effect
aspect ratio
Opis:
Cables may suffer severe damage in cable-stayed bridges, leading to cable relaxation and tension loss. Such a damage effect merges in the sag augmentation or tension tightened by the thermal effect. This paper is intended to investigate the static response of damaged cables coupled with the thermal effect. With the introduction of three damage parameters such as damage position, damage degree and damage range as well as temperature parameter, the dimensionless governing equations for cables are derived and the numerical method is employed to solve the nonlinear equations. The static behavior of damaged cables due to the thermal coupling effect is analyzed, and the way to remove the thermal effect is given. It shows that the aspect ratio is the major factor is the deflection of the mid-span and horizontal force in the cable, whereas the angle inclination is the next important parameter. Cables with the natural length close to the chord distance reveal the highest sensitivity to temperature, whereas pre-tensioned and non-pre-tensioned cables are less sensitive to the thermal effect. It further demonstrates that with more damage included, the sensitive scope of the variation coefficient of the axial force with aspect ratio and temperature changes dramatically.
Źródło:
Journal of Theoretical and Applied Mechanics; 2014, 52, 4; 1071-1082
1429-2955
Pojawia się w:
Journal of Theoretical and Applied Mechanics
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Prokaryotic expression, purification and antigenicity analysis of African swine fever virus pK205R protein
Autorzy:
Wu, X.
Xiao, L.
Peng, B.
Wang, Y.
Yang, Z.
Yao, X.
Hu, L.
Lin, X.
Powiązania:
https://bibliotekanauki.pl/articles/30963.pdf
Data publikacji:
2016
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
African swine fever is an acute, febrile and highly virulent porcine disease causing serious economic losses worldwide. The pK205R protein of the African swine fever virus (ASFV) is largely expressed in the early stages of infection, which has given the K205R gene extensive attention. In this study, the ASFV K205R was cloned and expressed in Escherichia coli BL21 (DE3). Expression of histidine-tagged pK205R with a molecular mass of 44 kDa was determined by 12% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and Western blot analysis. Optimisation of culture conditions allowed induction of the recombinant protein with 0.4 mM Isopropyl β-D-thiogalactoside (IPTG) at 37oC for 2 h. The protein existed in cellular supernatant and was purified using a Ni-NTA resin column. The purified protein was used to immunize rabbits four times to enable the production of polyclonal antibodies, and the antiserum titre was detected by ELISA. The results showed that the purified pK205R can react with ASFV positive serum specifically by Western blotting. The pK205R had high antigenicity, which indicated that pK205R could be used as an antigen for detection of ASFV-specific antibodies in ELISA testing, and the recombinant protein could contribute to further research of the action and structure of pK205R.
Źródło:
Polish Journal of Veterinary Sciences; 2016, 19, 1
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Reconstruction of oviraptorid clutches illuminates their unique nesting biology
Autorzy:
Yang, T.-R.
Wiemann, J.
Xu, L.
Cheng, Y.-N.
Wu, X.-C.
Sander, P.M.
Powiązania:
https://bibliotekanauki.pl/articles/19933.pdf
Data publikacji:
2019
Wydawca:
Polska Akademia Nauk. Instytut Paleobiologii PAN
Opis:
Oviraptorosaurs, a group of non-avian theropod dinosaurs from the Cretaceous of Asia and North America, left behind the most abundant and informative fossil evidence of dinosaur reproductive biology. Previous studies had suggested that oviraptorosaur reproductive biology represents an intermediate stage and exhibited unique modern avian traits. For instance, the adult-associated clutches were predominantly considered as evidence for brooding/thermoregulatory contact incubation (TCI) behaviors, whereas the hypotheses of laying or protection were neglected. Despite numerous oviraptorid egg clutches uncovered from China and Mongolia, their nest architecture and clutch arrangement were rarely investigated in detail. Here we present a comprehensive reconstruction of an oviraptorid clutch based on five new oviraptorid clutches from Jiangxi Province, China. A detailed examination of the new clutches reveals a partially-open oviraptorid nest that contains 3–4 rings of paired eggs (more than 15 pairs total) whose blunt end points toward the center devoid of eggs at an angle of 35–40°. Our detailed three-dimensional reconstruction indicates that the oviraptorid clutch has a unique architecture unknown from extant bird clutches, implying an apomorphic nesting mode. Such a unique nest architecture further contradicts the TCI hypothesis in oviraptorids, hindering sufficient heat transfer to the inner (lower) ring(s) of eggs. Moreover, the size of the new oviraptorid clutches (>30 eggs) is significantly larger than that of the adult-associated clutches (<22 eggs), raising the alternative hypothesis that the adult-associated clutches were uncompleted. This clue thus supports the hypothesis that the clutch-associated oviraptorid adults possibly represent females after an oviposition before a catastrophic sandstorm/flooding burial.
Źródło:
Acta Palaeontologica Polonica; 2019, 64, 3
0567-7920
Pojawia się w:
Acta Palaeontologica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł

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