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    Tytuł:
    The total protein content, protein fractions and proteases activities of drone prepupae of Apis mellifera due to varrosis
    Autorzy:
    Żółtowska, K.
    Lipiński, Z.
    Dmitryjuk, M.
    Powiązania:
    https://bibliotekanauki.pl/articles/2146371.pdf
    Data publikacji:
    2005
    Wydawca:
    Polskie Towarzystwo Parazytologiczne
    Tematy:
    protease
    protease activity
    honey bee
    drone prepupa
    protein fraction
    varrosis
    total protein
    Varroa destructor
    Apis mellifera
    protein content
    Opis:
    The proteins level and activities of acid and alkaline proteases in whole body extracts of drone prepupae of Apis mellifera naturaly infested with Varroa destructor were studied. The infested and a non-infested group did not differ significantly in their total protein content. However, some differences in protein profiles were found. A lack of three protein fractions of moderate and lower molecular weight in infested prepupae was noted. Moreover, some differences in the quantity of protein in most of the fractions were observed. The activity of acid proteases from infested prepupae was lower (p < 0.05) compared with the activity of these proteases from the non-infested one group. The infested drone had higher activity of alkaline proteases than non-infested but this difference was not statisticaly significant.
    Źródło:
    Wiadomości Parazytologiczne; 2005, 51, 1; 43-47
    0043-5163
    Pojawia się w:
    Wiadomości Parazytologiczne
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    The total protein content, protein fractions and proteases activities of drone prepupae of Apis mellifera due to varrosis
    Autorzy:
    Zoltowska, K
    Lipinski, Z.
    Dmitryjuk, M.
    Powiązania:
    https://bibliotekanauki.pl/articles/840749.pdf
    Data publikacji:
    2005
    Wydawca:
    Polskie Towarzystwo Parazytologiczne
    Tematy:
    protease
    protease activity
    honey bee
    drone prepupa
    protein fraction
    varrosis
    total protein
    Varroa destructor
    Apis mellifera
    protein content
    Opis:
    The proteins level and activities of acid and alkaline proteases in whole body extracts of drone prepupae of Apis mellifera naturaly infested with Varroa destructor were studied. The infested and a non-infested group did not differ significantly in their total protein content. However, some differences in protein profiles were found. A lack of three protein fractions of moderate and lower molecular weight in infested prepupae was noted. Moreover, some differences in the quantity of protein in most of the fractions were observed. The activity of acid proteases from infested prepupae was lower (p < 0.05) compared with the activity of these proteases from the non-infested one group. The infested drone had higher activity of alkaline proteases than non-infested but this difference was not statisticaly significant.
    Źródło:
    Annals of Parasitology; 2005, 51, 1
    0043-5163
    Pojawia się w:
    Annals of Parasitology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Przemiany substancji organicznej azotowej w zasiarczonej glebie plowej wzbogaconej gnojowica
    Tranformations of nitrogenous organic matter in sulfated lessive soil amended with liquid manure
    Autorzy:
    Jezierska-Tys, S
    Powiązania:
    https://bibliotekanauki.pl/articles/1401771.pdf
    Data publikacji:
    2000
    Wydawca:
    Polska Akademia Nauk. Instytut Agrofizyki PAN
    Tematy:
    gleby plowe
    proteaza
    ureaza
    aktywnosc proteolityczna
    aktywnosc ureazowa
    amonifikacja
    nitryfikacja
    zasiarczenie gleby
    gnojowica
    nawozenie gnojowica
    grey-brown podzolic soil
    protease
    urease
    proteolytic activity
    urease activity
    ammonification
    nitrification
    soil sulphation
    slurry
    slurry fertilization
    Opis:
    Celem przeprowadzonych badań w doświadczeniu modelowym na glebie płowej wytworzonej z piasku gliniastego mocnego było poznanie wpływu dawki 500 kg S ha-1 r-1 na aktywność proteolityczną, urolityczną, nasilenie amonifikacji i nitryfikacji. Przed wprowadzeniem dawki kwaśnego opadu glebę wzbogacono gnojowicą (60 m3 ha-1 r-1). Przeprowadzone badania wykazały, że najwyższą aktywnością proteolityczną charakteryzowała się gleba wzbogacona gnojowicą z równoczesnym zasiarczeniem. Zasiarczenie gleby oraz wzbogacenie gnojowicą również wpływało stymulująco na aktywność proteolityczną. Na aktywność ureazową gleby największy istotny wpływ miała gnojowica oraz, gnojowica z równoczesnym zasiarczeniem. Na nasilenie procesu amonifikacji hamujący wpływ miało zastosowane zasiarczenie i gnojowica. Natomiast na proces nitryfikacji istotnie stymulujące wpływały zarówno nawożenie w postaci gnojowicy jak i zastosowana dawka kwaśnego opadu.
    The experiment was set on lessive soil developed from heavy loamy sand.The aim of study was to evaluate the effect of 500 kg S ha-1 year -1 dose on proteolytical and urolytical activities, as well as intensification of amonification and nitrification processes. Soil was amended with liquid manure (60 m3 ha-1year-1)before acidic rainfall dose application.Studies revealed that soil amended with the liquid manure along with sulfating was characterized with the highest proteolytical activity. Soil sulfating and liquid manure amendment also stimulated the proteolytical activity.Soil sulfating and liquid manure amendment also stimulated the proteolytical activity. Liquid manure as well as liquid manure along with sulfating had the highest impact on urease activity. Sulfating and liquid manure applied inhibited the intensification of amonification process. However, nitrification process was stimulated by both liquid manure fertilization and acidic rainfall dose applied.
    Źródło:
    Acta Agrophysica; 2000, 38; 105-115
    1234-4125
    Pojawia się w:
    Acta Agrophysica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Influence of Anisakis simplex stage III larvae upon the activity of proteases under in vitro conditions
    Autorzy:
    Dziekonska-Rynko, J
    Rokicki, J.
    Jablonowski, Z.
    Powiązania:
    https://bibliotekanauki.pl/articles/837165.pdf
    Data publikacji:
    2002
    Wydawca:
    Polskie Towarzystwo Parazytologiczne
    Tematy:
    protease activity
    stage
    papain
    pepsin
    trypsin
    larva
    Anisakis simplex
    in vitro
    Opis:
    The larvae of Anisakis simplex had the largest influence upon decreasing the activity of porcine pepsin. The activity of that enzyme in tests, where the larvae were present during the entire period of incubation, was lower than in the controls. No similar trends were observed in case of the solutions with bovine and porcine trypsin. The activity of those enzymes in the solutions containing the larvae was higher than in the controls. Only the activity of porcine trypsin after 10 h of incubation was slightly lower in the experimental sample than in the control, however, during the later hours the dynamics of the activity decrease of that enzyme in the controls was higher than in the experimental samples. The recorded activity of papain in the samples containing the larvae was higher than that in the controls during the entire time of the experiment.
    Źródło:
    Annals of Parasitology; 2002, 48, 2
    0043-5163
    Pojawia się w:
    Annals of Parasitology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Influence of Anisakis simplex stage III larvae upon the activity of proteases under in vitro conditions
    Autorzy:
    Dziekońska-Rynko, J.
    Rokicki, J.
    Jabłonowski, Z.
    Powiązania:
    https://bibliotekanauki.pl/articles/2147699.pdf
    Data publikacji:
    2002
    Wydawca:
    Polskie Towarzystwo Parazytologiczne
    Tematy:
    protease activity
    stage
    papain
    pepsin
    trypsin
    larva
    Anisakis simplex
    in vitro
    Opis:
    The larvae of Anisakis simplex had the largest influence upon decreasing the activity of porcine pepsin. The activity of that enzyme in tests, where the larvae were present during the entire period of incubation, was lower than in the controls. No similar trends were observed in case of the solutions with bovine and porcine trypsin. The activity of those enzymes in the solutions containing the larvae was higher than in the controls. Only the activity of porcine trypsin after 10 h of incubation was slightly lower in the experimental sample than in the control, however, during the later hours the dynamics of the activity decrease of that enzyme in the controls was higher than in the experimental samples. The recorded activity of papain in the samples containing the larvae was higher than that in the controls during the entire time of the experiment.
    Źródło:
    Wiadomości Parazytologiczne; 2002, 48, 2; 217-223
    0043-5163
    Pojawia się w:
    Wiadomości Parazytologiczne
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    In silico studies of selected xanthophylls as potential candidates against SARS-CoV-2 targeting main protease (Mpro) and papain-like protease (PLpro)
    Autorzy:
    Karpiński, T.M.
    Kwaśniewski, M.
    Ożarowski, M.
    Alam, R.
    Powiązania:
    https://bibliotekanauki.pl/articles/2049355.pdf
    Data publikacji:
    2021
    Wydawca:
    Instytut Włókien Naturalnych i Roślin Zielarskich
    Tematy:
    protease
    papain-like protease
    xanthophyll
    coronavirus
    COVID-19
    antiviral activity
    koronawirus
    pandemia
    działanie przeciwwirusowe
    projektowanie leków wspomagane komputerowo
    Opis:
    Introduction: The main protease (Mpro) and the papain-like protease (PLpro) are essential for the replication of SARS-CoV-2. Both proteases can be targets for drugs acting against SARS-CoV-2. Objective: This paper aims to investigate the in silico activity of nine xanthophylls as inhibitors of Mpro and PLpro. Methods: The structures of Mpro (PDB-ID: 6LU7) and PLpro (PDB-ID: 6W9C) were obtained from RCSB Protein Data Bank and developed with BIOVIA Discovery Studio. Active sites of proteins were performed using CASTp. For docking the PyRx was used. Pharmacokinetic parameters of ADMET were evaluated using SwissADME and pkCSM. Results: β-cryptoxanthin exhibited the highest binding energy: –7.4 kcal/mol in the active site of Mpro. In PLpro active site, the highest binding energy had canthaxanthin of –9.4 kcal/mol, astaxanthin –9.3 kcal/mol, flavoxanthin –9.2 kcal/mol and violaxanthin –9.2 kcal/mol. ADMET studies presented lower toxicity of xanthophylls in comparison to ritonavir and ivermectin. Conclusion: Our findings suggest that xanthophylls can be used as potential inhibitors against SARS-CoV-2 main protease and papain-like protease.
    Źródło:
    Herba Polonica; 2021, 67, 2; 1-8
    0018-0599
    Pojawia się w:
    Herba Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Immobilization of Bacillus megaterium in Carrageenan from Maluku Sea and Their Effect on Protease Production
    Autorzy:
    Hamdani, Syarif
    Nurlatifah, Sri
    Astriany, Dewi
    Singgih, Marlia W.
    Ibrahim, Slamet W.
    Powiązania:
    https://bibliotekanauki.pl/articles/1811540.pdf
    Data publikacji:
    2020
    Wydawca:
    Politechnika Koszalińska. Wydawnictwo Uczelniane
    Tematy:
    carrageenan
    immobilization
    protease activity
    Bacillus megaterium
    Opis:
    Bacteria immobilized in carrageenan are widely used in industry to facilitate bacterial handling and storage. Carrageenan is derived from seaweed and its nature is influenced by the condition of the origin of the sea where seaweed grows, one of the Indonesia sea territories that has seaweed that contains caraganen with good properties is Maluku. This study was conducted to determine the effect of storage time of bacteria immobilized in Maluku sea’s carrageenan on proteolytic activity, the bacteria used were Bacillus megaterium. Bacterial immobilization of carrageenan was made at concentrations of 1%, 1.5%, and 2%, storage in cold conditions for up to 9 months. Protease activity was tested using Kunitz method by adding casein as a substrate. The optimal concentration of carrageenan for immobilization of Bacillus megaterium was obtained at a concentration of 1.5%. Protease isolated from immobilized Bacillus megaterium showed increased activity value from storage for 4 months (0.0489 Ug-1) to 7 months (0.1372 Ug-1), and decreased activity after being stored for 9 months (0.0501 Ug-1).
    Źródło:
    Rocznik Ochrona Środowiska; 2020, Tom 22, cz. 1; 60-69
    1506-218X
    Pojawia się w:
    Rocznik Ochrona Środowiska
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Extrachromosomal expression of nat05 gene encoding an alkaline serine protease from Bacillus subtilis N05
    Autorzy:
    Thu, N.T.A.
    Chau, N.T.T.
    Thien, L.V.
    Huy, N.D.
    Khue, N.T.M.
    Hung, N.B.
    Luong, N.N.
    Thu, L.T.A.
    Loc, N.H.
    Powiązania:
    https://bibliotekanauki.pl/articles/80935.pdf
    Data publikacji:
    2018
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    gene encoding
    serine protease
    Bacillus subtilis
    nat05 gene
    fibrinolytic activity
    alkaline serine protease
    sodium dodecyl sulphate-polyacrylamide gel electrophoresis
    proteolytic enzyme
    Źródło:
    BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2018, 99, 4
    0860-7796
    Pojawia się w:
    BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Enzymatic activity of actinomycetes from the genus Streptomyces isolated from the bulk soil and rhizosphere of the Pinus sylvestris
    Autorzy:
    Golinska, P.
    Dahm, H.
    Powiązania:
    https://bibliotekanauki.pl/articles/41609.pdf
    Data publikacji:
    2011
    Wydawca:
    Polska Akademia Nauk. Instytut Dendrologii PAN
    Tematy:
    enzyme activity
    Actinomycetes
    Streptomyces
    isolation
    bulk soil
    forest soil
    rhizosphere
    Pinus sylvestris
    chitinase
    protease
    pectinase
    cellulase
    Opis:
    The enzymatic activity (chitinolytic, proteolytic, pectolytic andcellulolytic) of twenty strains of Streptomyces isolated from soil and rhizosphere of Scots pine was analyzed. Most strains produced chitinases, catalyzing the degradation of chitin, the main component of fungal cell walls including fungi pathogenic for plants. This activity was about 4 times higher in the presence of Fusariumoxysporum than Rhizoctonia solani mycelium or chitin flakes. The number of the proteolytic strains was also significant. In general, rhizosphere and soil organisms synthesizedcomparable amounts of these enzymes. Over half of the analyzed Streptomyces strains produced pectolytic enzymes (polygalacturonase, pectin lyase and pectate lyase). This property was more common among rhizosphere than among soil strains. The Streptomyces strains also showedcellulolytic activity (endocellulases, exocellulases) – enzymes decomposing basic components of cell walls of plant and lower fungi (cellulose). The cellulolytic activity was differentiated and depended on the Streptomyces strain. Conclusion of our studies is that Streptomyces are the microorganisms more chitinolytic andproteolytic than pectolytic and cellulolytic.
    Źródło:
    Dendrobiology; 2011, 65
    1641-1307
    Pojawia się w:
    Dendrobiology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Effect of new lines of winter wheat on microbiological activity in Luvisol
    Autorzy:
    Jezierska-Tys, S.
    Rachon, L.
    Rutkowska, A.
    Szumilo, G.
    Powiązania:
    https://bibliotekanauki.pl/articles/24850.pdf
    Data publikacji:
    2012
    Wydawca:
    Polska Akademia Nauk. Instytut Agrofizyki PAN
    Tematy:
    new line
    winter wheat
    wheat
    microbiological activity
    Luvisol
    soil
    bacteria
    fungi
    protease
    urease
    ammonification
    nitrification
    plant
    root zone
    microorganism
    Opis:
    The study presented in this paper was conducted under the conditions of a field experiment. Microbiological analyses were made at various stages of winter wheat plants developmentie heading, milk ripeness and full ripeness. The objective of the study was to acquire knowledge on the effect of cultivation of various lines of winter wheat on the numbers of bacteria and fungi with proteolytic capabilities, on protease and urease activity, and on the rate of the processes of ammonification and nitrification. The results of conducted study demonstrated that the number of proteolytic bacteria and fungi, as well as the activity of protease and urease, and the intensity of ammonification and nitrification processes in soil depended on both the development stage and cultivated line of winter wheat.
    Źródło:
    International Agrophysics; 2012, 26, 1
    0236-8722
    Pojawia się w:
    International Agrophysics
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Bioactive metabolites produced by Spirulina subsalsa from the Baltic Sea
    Autorzy:
    Szubert, K.
    Wiglusz, M.
    Mazur-Marzec, H.
    Powiązania:
    https://bibliotekanauki.pl/articles/47501.pdf
    Data publikacji:
    2018
    Wydawca:
    Polska Akademia Nauk. Instytut Oceanologii PAN
    Tematy:
    bioactive metabolite
    Spirulina subsalsa
    Baltic Sea
    Cyanoprokaryota
    bioremediation
    cytotoxic activity
    liquid chromatography-tandem mass spectrometry
    protease inhibitor
    Źródło:
    Oceanologia; 2018, 60, 3
    0078-3234
    Pojawia się w:
    Oceanologia
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    AtDeg2 - a chloroplast protein with dual protease/chaperone activity
    Autorzy:
    Jagodzik, P.
    Adamiec, M.
    Jackowski, G.
    Powiązania:
    https://bibliotekanauki.pl/articles/57301.pdf
    Data publikacji:
    2014
    Wydawca:
    Polskie Towarzystwo Botaniczne
    Tematy:
    chloroplast protein
    protease
    proteolytic enzyme
    chaperone
    enzyme activity
    chloroplast
    PDZ domain
    Opis:
    Chloroplast protease AtDeg2 (an ATP-independent serine endopeptidase) is cytosolically synthesized as a precursor, which is imported into the chloroplast stroma and deprived of its transit peptide. Then the mature protein undergoes routing to its functional location at the stromal side of thylakoid membrane. In its linear structure AtDeg2 molecule contains the protease domain with catalytic triad (HDS) and two PDZ domains (PDZ1 and PDZ2). In vivo AtDeg2 most probably exists as a supposedly inactive haxamer, which may change its oligomeric stage to form active 12-mer, or 24-mer. AtDeg2 has recently been demonstrated to exhibit dual protease/chaperone function. This review is focused on the current awareness with regard to AtDeg2 structure and functional significance.
    Źródło:
    Acta Societatis Botanicorum Poloniae; 2014, 83, 3
    0001-6977
    2083-9480
    Pojawia się w:
    Acta Societatis Botanicorum Poloniae
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Alkaline trypsin from the viscera and heads of Engraulis anchoita: partial purification and characterization
    Autorzy:
    Lamas, D.L.
    Yeannes, M.I.
    Massa, A.E.
    Powiązania:
    https://bibliotekanauki.pl/articles/951322.pdf
    Data publikacji:
    2017
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    alkaline protease
    trypsin
    viscera
    head
    Engraulis anchoita
    purification
    proteinase activity
    ethylenediaminetetraacetic acid
    sodium dodecyl sulphate-polyacrylamide gel electrophoresis
    proteolytic enzyme
    Źródło:
    BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2017, 98, 2
    0860-7796
    Pojawia się w:
    BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Aktywnosc proteolityczna i amonifikacyjna uzytkowanych rolniczo gleb zlewni jeziora Piaseczno i Glebokie [Pojezierze Leczynsko-Wlodawskie]
    Proteolytic and ammonification activity of cultivated soils of the piaseczno and glebokie catchment basin (The Leczynsko-Wlodawskie Lake District)
    Autorzy:
    Szwed, A
    Furczak, J.
    Powiązania:
    https://bibliotekanauki.pl/articles/1401802.pdf
    Data publikacji:
    2000
    Wydawca:
    Polska Akademia Nauk. Instytut Agrofizyki PAN
    Tematy:
    Pojezierze Leczynsko-Wlodawskie
    Jezioro Glebokie
    jezioro Piaseczno
    zlewnie jeziorne
    gleby
    gleby bielicowe
    proteaza
    aktywnosc proteolityczna
    amonifikacja
    aktywnosc amonifikacyjna
    Leczynsko-Wlodawskie Lakeland
    Lake Glebokie
    Lake Piaseczno
    lake catchment
    soil
    podzolic soil
    protease
    proteolytic activity
    ammonification
    ammonification activity
    Opis:
    Badania realizowano w glebie bielicowej wytworzonej z piasku luźnego, która cechuje się niewielką żyznością oraz w czarnej ziemi wytworzonej z piasku słabo gliniastego, która posiada dużą ilość części spławialnych, wysoki poziom węgla i azotu organicznego oraz znaczną pojemność sorpcyjną. Gleby te występują w sektorze rolniczym zlewni dwu jezior - Piaseczno i Głębokie (Pojezierze Łęczyńsko-Włodawskie). Próby do analiz biochemicznych pobierano 7, różnych stanowisk czterokrotnie w następujących terminach: kwiecień, maj, lipiec i wrzesień. Aktywność proteazy w glebach badano metodą Ladda i Butlera. Natomiast nasilenie procesu amonifikacji określano na podstawie przyrostu zawartości N-NH4+ (w próbkach glebowych inkubowanych z 0,1 % asparaginy) oznaczonego metodą Nesslera. Przeprowadzone badania wykazały, że aktywność proteolityczna zależała od właściwości gleby. W glebie bielicowej kształtowała się na ogół na niższym poziomie w porównaniu z czarną ziemią i była zróżnicowana w zależności od odległości stanowiska od jeziora. Potencjalna zdolność amonifikacyjna gleb nie była wyraźnie powiązana z ich właściwościami. W glebie bielicowej utrzymywała się w całym sektorze na zbliżonym poziomie. Natomiast w przypadku czarnej ziemi stwierdzono zależność badanego procesu od punktu pobrania gleby. Najwyższą wartość tej aktywności odnotowano w glebie zlokalizowanej na brzegu jeziora.
    The investigations were carried out in podzolic soil of poor fertility developed from loose sand and black soil formed from slightly loamy sand with great part of fine fraction, high level of carbon and organic nitrogen and considerable sorp tivity. The soils arc located in the agricultural sector of the two lakes basin - Piaseczno and Głębokie (the Łęczyńsko-Włodawskie Lake District),The samples for biochemical analyses were gathered from different test sites for four times in the following terms: April, May, July and September, The protease activity in soils were examined after Ladd and Butler's method. Intensification of ammonification process was determined on die basis of N-NH4+ content growth (in soil samples incubated with 0,1 % asparagine) determined by Nesslef s method. The examinations proved the proteolytic activity depended on soil properties. In podzolic soil it demonstrated a lower level as compared to black soil and was differentiated in relation to test site distance off a lake. Potential ammonification activity of soils was not clearly connected with their characteristics. In podzolic .soil it maintained a similar level all over the agricultural sector, while in black soil there was stated dependence of a process examined on a sampling point. The highest value of the activity was recorded in the soil located on the lake bank.
    Źródło:
    Acta Agrophysica; 2000, 38; 237-246
    1234-4125
    Pojawia się w:
    Acta Agrophysica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
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