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Wyświetlanie 1-15 z 143
Tytuł:
Adaptation of PCR technique for quantitative estimation of genetic material from different regions of chromosome 21 in cases of trisomy 21.
Autorzy:
Nowacka, Joanna
Helszer, Zofia
Walter, Zofia
Płucienniczak, Andrzej
Kałużewski, Bogdan
Powiązania:
https://bibliotekanauki.pl/articles/1041513.pdf
Data publikacji:
2004
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
quantitative polymerase chain reaction
trisomy 21
Opis:
Pre- and postnatal diagnosis of chromosomal aberrations is generally based on conventional cytogenetic analysis. In this paper, we have devised a quantitative polymerase chain reaction (Q-PCR) method to determine gene dose effects and applied it in cases of regular trisomy 21 as a model. The method is based on quantitative assessment of PCR products after using primers amplifying DNA fragments located in the pericentromeric, heterochromatic, euchromatic and telomeric regions of chromosome 21. A gene dose effect on the amount of PCR product in cases of trisomy 21 was confirmed. Moreover, a correlation between the amount of the PCR product of the examined sequences and their location in the chromosome was observed. The obtained results suggest that the Q-PCR technique can be applied in the diagnosis of aneuploidies.
Źródło:
Acta Biochimica Polonica; 2004, 51, 4; 995-1001
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Improved method of isolation of total nucleic acids from hop plants and grapevine before the RT-PCR by addition of polyvinylpolypyrrolidone
Usprawniona metoda izolacji calkowitych kwasow nukleinowych z chmielu i winorosli przez RT-PCR przez dodanie poliwinylopolipirolidonu
Autorzy:
Cajza, M
Folkman, W.
Powiązania:
https://bibliotekanauki.pl/articles/65582.pdf
Data publikacji:
2003
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
isolation
total nucleic acid
nucleic acid
hop plant
grapevine
RT-PCR method zob.reverse transcription polymerase chain reaction
addition
polyvinylpolypyrrolidone
reverse transcription polymerase chain reaction
polymerase chain reaction
Źródło:
Journal of Plant Protection Research; 2003, 43, 4; 375-380
1427-4345
Pojawia się w:
Journal of Plant Protection Research
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Polymorphism in Syringa rDNA regions assessed by PCR technique
Autorzy:
Smolik, M.
Andrys, D.
Franas, A.
Krupa-Malkiewicz, M.
Malinowska, K.
Powiązania:
https://bibliotekanauki.pl/articles/41641.pdf
Data publikacji:
2010
Wydawca:
Polska Akademia Nauk. Instytut Dendrologii PAN
Tematy:
polymorphism
lilac
Syringa
rDNA region
polymerase chain reaction
Opis:
The Syringa genus is characterizedby a multiplicity of forms. Its chief asset is the ornamental value of thousands of accessions, species or hybrids. From a phylogenetic point of view the genus is difficult in an explicit classification due to its frequently complex genome. The aim of this study was to determine the possibility for the identification of genotypic diversity and genetic relationships in the nrDNA sequence of some selected Syringa accessions – part of a collection of the Dendrological Garden in Przelewice (Poland). For this purpose, the PCR technique together with a combination of various ‘universal’ primers designed for the nrDNA sequence analysis were employed. Fourteen Syringa accessions: Syringa × chinensis Willd., S. × prestoniae Mc Kelv., S. × prestoniae ‘Telimena’, S. × prestoniae ‘Jaga’, S. × prestoniae ‘Basia’, S. meyeri ‘Palibin’, S. vulgaris ‘Miss Ellen Willmott’, S. vulgaris, S. vulgaris ‘Jules Simon’, S. vulgaris ‘Katherine Havemeyer’, S. vulgaris ‘Krasawica Moskvy’, S. vulgaris ‘Mirabeau’, S. vulgaris ‘Madame Lemoine’ and S. vulgaris ‘Niebo Moskvy’ made up the research material. In the conducted amplifications, genetic profiles were obtained for 14 combinations among the 25 combinations of different pairs of primers used. The nrDNA templates coding the small subunit (SSU), 5.8S subunit andITS1, ITS2 andIGS sequences were amplified. In PCR reactions a total of 33 PCR products were generated, of which 21 (64%) products were polymorphic, 6 (18%) monomorphic and6 (18%) were genotype-specific. For the lilac accessions examined246 amplicons were generated from ~230 to ~1100 bp in length. The analysis of both the dendrogram and the genetic similarity matrix revealedlow diversity between the examinedaccessions. For most they rangedfrom 70 to 80%, andthe greatest diversity (87%) was foundbetween the S. × prestoniae: ‘Basia’ and‘Telimena’ accessions, while the lowest (57%) was observed between S. vulgaris ‘Katherine Havermeyer’ and S. × chinensis.
Źródło:
Dendrobiology; 2010, 64
1641-1307
Pojawia się w:
Dendrobiology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Application of polymerase chain reaction-restriction fragment length polymorphism (RFLP-PCR) in the analysis of single nucleotide polymorphisms (SNPs)
Autorzy:
Tarach, Piotr
Powiązania:
https://bibliotekanauki.pl/articles/1830648.pdf
Data publikacji:
2021-09-29
Wydawca:
Uniwersytet Łódzki. Wydawnictwo Uniwersytetu Łódzkiego
Tematy:
nucleotide polymorphisms
DNA analysis
polymerase chain reaction
Opis:
Polymerase chain reaction-restriction fragment length polymorphism (RFLP-PCR) is a technique used to identify single nucleotide polymorphisms (SNPs) based on the recognition of restriction sites by restriction enzymes. RFLP-PCR is an easy-to-perform and inexpensive tool for initial analysis of SNPs potentially associated with some monogenic diseases, as well as in genotyping, genetic mapping, lineage screening, forensics and ancient DNA analysis. The RFLP-PCR method employs four steps: (1) isolation of genetic material and PCR; (2) restriction digestion of amplicons; (3) electrophoresis of digested fragments; and (4) visualisation. Despite its obsolescence and the presence of high-throughput DNA analysis techniques, it is still applied in the analysis of SNPs associated with disease entities and in the analysis of genetic variation of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). RFLP-PCR is a low-cost and low-throughput research method allowing for the analysis of SNPs in the absence of specialised equipment, and it is useful when there is a limited budget.
Źródło:
Acta Universitatis Lodziensis. Folia Biologica et Oecologica; 2021, 17; 48-53
1730-2366
2083-8484
Pojawia się w:
Acta Universitatis Lodziensis. Folia Biologica et Oecologica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Molecular diagnostics of Sarcocystis spp. infections
Autorzy:
Stojecki, K.
Karamon, J.
Sroka, J.
Cencek, T.
Powiązania:
https://bibliotekanauki.pl/articles/2088002.pdf
Data publikacji:
2012
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
molecular diagnostics
Sarcocystis
infection
sarcocystosis
polymerase chain reaction
Opis:
Protozoa of the genus Sarcocystis (phylum Apicomplexa, family Sarcocystidae) is one of the most common parasites affecting animals. Interspecies diagnostic of Sarcocystis genus was based on electron microscopy for many years. Because of absence of visible differences between species with reachable magnifications, light microscopy is useless. In many cases serological diagnostic method have lack of sensitivity. A variety of molecular methods have been developed and used to detect and identify Sarcocystis spp. and to assess the genetic diversity among this protozoan from different population/hosts. Nowadays, molecular diagnostic is the common, time/cost effective method used all over the world to interspecies differentiation.
Źródło:
Polish Journal of Veterinary Sciences; 2012, 15, 3
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Advanced methods of bacteriological identification in a clinical microbiology laboratory
Autorzy:
Zukowska, M.E.
Powiązania:
https://bibliotekanauki.pl/articles/2098275.pdf
Data publikacji:
2020
Wydawca:
Instytut Medycyny Wsi
Tematy:
clinical microbiology
molecular method
modern method
multiplex polymerase chain reaction
real-time polymerase chain reaction
next generation sequencing
MALDI-TOF mass spectrometry
Opis:
Introduction and objective. Conventional, culture-based methods of bacterial identification and drug-susceptibility testing are considered the gold standard in medical microbiology. In recent years, classical microbiological methods have been supplemented with modern analytical and molecular methods. The aim of the review was to discusses the methods which have been permanently adapted to bacteriological microbiological diagnostics. Abbreviated description of the state of knowledge. Currently, PCR, as well as other nucleic acid amplification tests and sequencing techniques, are part of the standard repertoire of microbiological diagnostics. With regard to the quality and speed of pathogen identification, the introduction of mass spectrometry techniques into routine microbiological diagnostics work-up has been revolutionary. Within a short time in many laboratories, Matrix-Assisted Laser Desorption/Ionisation – Time of Flight Mass Spectrometry (MALDI TOF MS) systems have almost completely replaced conventional biochemical pathogen identification. Conclusions. Microbiological diagnostics is an indispensable element of a targeted therapy. The techniques used in the laboratory depend primarily on the laboratory’s apparatus, the costs of the analysis, as well as the sensitivity and specificity of a method. However, regardless of the culture-based methods universality, advanced techniques have permanently established themselves in diagnostics. Confident information about the detected organism and treatment possibilities in a combination with the clinical context are conducive to successful therapy. Although modern methods still require validation and close collaboration between clinicians, microbiologists and bioinformaticians, these methods, once deemed to be the future, have already arrived.
Źródło:
Journal of Pre-Clinical and Clinical Research; 2021, 15, 2; 68-72
1898-2395
Pojawia się w:
Journal of Pre-Clinical and Clinical Research
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Detection of bovine leucocyte adhesion deficiency [BLAD] carriers using a new PCR test
Autorzy:
Kaminski, S
Czarnik, U
Powiązania:
https://bibliotekanauki.pl/articles/2046599.pdf
Data publikacji:
1997
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
cattle
bovine leucocyte adhesion deficiency
polymerase chain reaction
bacterial infection
Opis:
In this report we demonstrate a simple, effective and reliable diagnostic test of BLAD carrier detection based on specific PCR amplification of a 367 bp CD18 gene fragment and RFLP analysis using Taq I restriction enzyme. In a non-random population of 220 animals we found 48 BLAD carriers. Within the amplified PCR fragment an unknown intron sequence of 159 bp was identified.
Źródło:
Journal of Applied Genetics; 1997, 38, 1; 51-55
1234-1983
Pojawia się w:
Journal of Applied Genetics
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Vegetable oil plant wastewater treatment by bacterial isolates : a study in the city of Hila, Iraq
Autorzy:
Salim, Hanan Kareem
Al-Ahmed, Suad Ghali Kadhim
Powiązania:
https://bibliotekanauki.pl/articles/2048496.pdf
Data publikacji:
2021
Wydawca:
Instytut Technologiczno-Przyrodniczy
Tematy:
biotreatment
polymerase chain reaction
PCR
sewage
vegetable oil plant
wastewater
Opis:
The present study was to reflect the use of some bacteria in the treatment and removal of pollutants in three selected wastewater sites, including a vegetable oil plant (viz. Al-Etihad Food Industries), the main wastewater treatment station in the city of Hila, and Al-Hila River water from October 2019 to January 2020. The bacterial isolates identified in these three sites were Klebsiella pneumoniae, Escherichia coli, Enterobacteria cloacae, Pseudomonas aeruginosa, Thalasobacillus devorans, Acinetobacter baumannii, and Bacillus subtilis. The molecular study of the bacterial isolates involved the detection of bacterial genera using the polymerase chain reaction (PCR). The results showed that water had a variable nature, depending on the substances in it. It recorded varying chemical and physical property values, ranging between 6.36 and 7.82 for pH and from 2500 to 7100 mg∙dm-3 for total alkalinity. Additional values were 713–2051 μS∙cm-1 for electrical conductivity (EC), 5.90–9.80 mg∙dm-3 for chemical oxygen demand (COD), and 480–960 mg∙dm-3 for total hardness. The given values were also 0.20–0.65 μg∙dm-3, 0.03-0.23 μg∙dm-3, and 0–107 mg∙dm-3 for nitrite (NO2), phosphate (PO4) oils, respectively.
Źródło:
Journal of Water and Land Development; 2021, 51; 163-167
1429-7426
2083-4535
Pojawia się w:
Journal of Water and Land Development
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Hantavirus RNA not detected in Ixodes ricinus ticks
Autorzy:
Wojcik-Fatla, A.
Zajac, V.
Knap, J.P.
Dutkiewicz, J.
Powiązania:
https://bibliotekanauki.pl/articles/49890.pdf
Data publikacji:
2011
Wydawca:
Instytut Medycyny Wsi
Tematy:
hantavirus
RNA
Ixodes ricinus
tick
epidemiology
polymerase chain reaction
Polska
Źródło:
Annals of Agricultural and Environmental Medicine; 2011, 18, 2
1232-1966
Pojawia się w:
Annals of Agricultural and Environmental Medicine
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Isolation, Identification and Preservation of Pectinolytic Bacteria Pathogenic to Potato
Autorzy:
Lebecka, Renata
Powiązania:
https://bibliotekanauki.pl/articles/2199695.pdf
Data publikacji:
2017-06-20
Wydawca:
Instytut Hodowli i Aklimatyzacji Roślin
Tematy:
Blackleg
Dickeya
Pectobacterium
Polymerase Chain Reaction
selective medium
soft rot
Opis:
Blackleg of potato plants and soft rot of tubers are caused by several species of pectinolytic bacte-ria from genera Pectobacterium and Dickeya. The text describes simple methods of isolating bacteria from symptomatic and symptomless organs of potato plants, their identification using Polymerase Chain Reaction (PCR) and preservation.
Źródło:
Plant Breeding and Seed Science; 2017, 75; 87-96
1429-3862
2083-599X
Pojawia się w:
Plant Breeding and Seed Science
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The influence of LDHA gene polymorphism on relative level of its expression in racing pigeons
Polimorfizm w genie LDHA i jego wpływ na względny poziom ekspresji u gołębi pocztowych
Autorzy:
Jędrzejczak-Silicka, M.
Yu, Y.-H.
Cheng, Y.-H.
Dybus, A.
Powiązania:
https://bibliotekanauki.pl/articles/2606361.pdf
Data publikacji:
2018
Wydawca:
Zachodniopomorski Uniwersytet Technologiczny w Szczecinie. Wydawnictwo Uczelniane ZUT w Szczecinie
Tematy:
gene polymorphism
gene expression
pigeon
real-time polymerase chain reaction
Źródło:
Acta Scientiarum Polonorum. Zootechnica; 2018, 17, 3; 9-15
1644-0714
Pojawia się w:
Acta Scientiarum Polonorum. Zootechnica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Antimicrobial activities and phylogenetic study of Erythrina senegalensis DC (Fabaceae) seed lectin
Autorzy:
Enoma, Samuel
Adewole, Taiwo S.
Agunbiade, Titilayo O.
Kuku, Adenike
Powiązania:
https://bibliotekanauki.pl/articles/16672367.pdf
Data publikacji:
2023
Wydawca:
Polska Akademia Nauk. Czasopisma i Monografie PAN
Tematy:
antimicrobial
Erythrina senegalensis
lectin
phylogenetic analysis
polymerase chain reaction
Opis:
Erythrina senegalensis (Fabaceae) have been traditionally used in the treatment of microbial ailments, and the specific agent mediating its efficacy has been investigated in several studies. In this study, the antimicrobial activity of purified E. senegalensis lectin (ESL) was analyzed. The phylogenetic relationship of the gene encoding lectin with other legume lectins was also established to investigate their evolutionary relationship via comparative genomics. Antimicrobial activity of ESL against selected pathogenic bacteria and fungi isolates was evaluated by the agar well diffusion method, using fluconazole (1 mg/ml) and streptomycin (1 mg/ml) as positive controls for fungi and bacteria sensitivity, respectively. Potent antimicrobial activity of ESL against Erwinia carotovora, Pseudomonas aeruginosa, Klebsiella pneumonia, Staphylococcus aureus, Aspergillus niger, Penicillium camemberti, and Scopulariopsis brevicaulis was observed, with inhibition zones ranging from 18 to 24 mm. Minimum inhibitory concentrations of ESL ranged between 50 and 400 μg/ml. Primer-directed polymerase chain reaction of E. senegalensis genomic DNA detected a 465-bp lectin gene with an open reading frame encoding a 134-amino acid polypeptide. The obtained nucleotide sequence of the ESL gene shared high sequence homology: 100, 100, and 98.18% with Erythrina crista-galli, Erythrina corallodendron, and Erythrina variegata lectin genes, respectively, suggesting that the divergence of Erythrina lectins might follow species evolution. This study concluded that ESL could be used to develop lectin-based antimicrobials, which could find applications in the agricultural and health sectors.
Źródło:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2023, 104, 1; 21-32
0860-7796
Pojawia się w:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
DNA isolation from Cryptosporidium oocysts directly from stool samples for diagnostic goals using PCR
Autorzy:
Sulima, P.
Powiązania:
https://bibliotekanauki.pl/articles/840154.pdf
Data publikacji:
1998
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
diagnostic goal
stool
polymerase chain reaction
Cryptosporidium
DNA
oocyst
Źródło:
Annals of Parasitology; 1998, 44, 3
0043-5163
Pojawia się w:
Annals of Parasitology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
A universal method for the identification of genes encoding amatoxins and phallotoxins in poisonous mushrooms
Autorzy:
Woloszyn, A.
Kotlowski, R.
Powiązania:
https://bibliotekanauki.pl/articles/875623.pdf
Data publikacji:
2017
Wydawca:
Narodowy Instytut Zdrowia Publicznego. Państwowy Zakład Higieny
Tematy:
identification method
gene encoding
amatoxin
phallotoxin
poisonous mushroom
polymerase chain reaction
Opis:
Background. As the currently known diagnostic DNA targets amplified in the PCR assays for detection of poisonous mushrooms have their counterparts in edible species, there is a need to design PCR primers specific to the genes encoding amanitins and phallotoxins, which occur only in poisonous mushrooms. Objective. The aim of the study was testing of PCR-based method for detection of all genes encoding hepatotoxic cyclic peptides - amanitins and phallotoxins present in the most dangerous poisonous mushrooms. Material and Methods. Degenerate primers in the PCR were designed on the basis of amanitins (n=13) and phallotoxins (n=5) genes in 18 species of poisonous mushrooms deposited to Genbank of the National Center for Biotechnology Information. Results. The specificity of the PCR assays was confirmed against 9 species of edible mushrooms, death cap - Amanita phalloides and panther cap - Amanita pantherina. Conclusions. Designed two couples of PCR-primers specific to amanitins and phallotoxins genes can be recommended for detection of Amanita phalloides and other mushroom species producing hepatotoxic cyclic peptides - amanitins and phallotoxins.
Wprowadzenie. Ponieważ, obecnie znane diagnostyczne cele molekularne w genomach trujących grzybów kapeluszowych amplifikowane metodą PCR mają swoje odpowiedniki u grzybów jadalnych, istnieje potrzeba zastosowania specyficznych sekwencji starterowych wobec amanityn i fallotoksyn, występujących jedynie u grzybów trujących. Cel. Celem prowadzonych badań było sprawdzenie przydatności sekwencji starterowych do reakcji PCR specyficznych wobec wszystkich aktualnie poznanych genów kodujących hepatotoksyczne cykliczne peptydy - amanityny oraz fallotoksyny trujących grzybów kapeluszowych. Materiał i Metody. Sekwencje oligonokleotydowe starterów do reakcji PCR zaprojektowane zostały w oparciu o zdeponowane w Genbanku geny amanityn (n=13) oraz fallotoksyn (n=5). Wyniki. Specyficzność opracowanych testów PCR potwierdzono wobec 9 gatunków grzybów jadalnych oraz muchomora sromotnikowego - Amanita phalloides, jak i muchomora plamistego - Amanita pantherina. Wnioski. Zastosowane sekwencje starterowe do wykrywania genów kodujących amanityny i fallotoksyny metodą PCR, mogą być wykorzystane do wykrywania muchomora sromotnikowego oraz innych gatunków zdolnych do syntezy amanityn oraz fallotoksyn.
Źródło:
Roczniki Państwowego Zakładu Higieny; 2017, 68, 3
0035-7715
Pojawia się w:
Roczniki Państwowego Zakładu Higieny
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Principles and applications of Ligation Mediated PCR methods for DNA-based typing of microbial organisms
Autorzy:
Krawczyk, Beata
Kur, Józef
Stojowska-Swędrzyńska, Karolina
Śpibida, Marta
Powiązania:
https://bibliotekanauki.pl/articles/1038839.pdf
Data publikacji:
2016
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
rapid methods
molecular epidemiology
genotyping
microbial phylogenetics
PCR (polymerase chain reaction)
Opis:
A significant number of DNA-based techniques has been introduced into the field of microorganisms' characterization and taxonomy. These genomic fingerprinting methods were developed to detect DNA sequence polymorphisms by using general principles, such as restriction endonuclease analysis, molecular hybridization, and PCR amplification. In recent years, some alternative techniques based on ligation of oligonucleotide adapters before DNA amplification by PCR, known as Ligation-Mediated PCR methods (LM PCR), have been successfully applied for the typing of microorganisms below the species level. These molecular methods include: Amplified Fragment Length Polymorphism (AFLP), Amplification of DNA fragments Surrounding Rare Restriction Sites (ADSRRS), PCR Melting Profiles (PCR MP), Ligation Mediated PCR/Shifter (LM PCR/Shifter), Infrequent-Restriction-Site Amplification (IRS PCR), double digestion Ligation Mediated Suppression PCR (ddLMS PCR). These techniques are now applied more and more often because they involve less time, are comparably inexpensive, and require only standard lab equipment. Here, we present a general review of this group of methods showing their possibilities and limitations. We also identify questions and propose solutions which may be helpful in choosing a particular LM PCR method for the achievement of the required goal.
Źródło:
Acta Biochimica Polonica; 2016, 63, 1; 39-52
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Wyświetlanie 1-15 z 143

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