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Tytuł:
Physical properties of lipid bilayer membranes: relevance to membrane biological functions.
Autorzy:
Subczynski, Witold
Wisniewska, Anna
Powiązania:
https://bibliotekanauki.pl/articles/1044298.pdf
Data publikacji:
2000
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
lipid exchange
hydrophobic barrier
oxygen transport
lipid unsaturation
membrane domains
lipid bilayer
Opis:
Over the last 25 years one of us (WKS) has been investigating physical properties of lipid bilayer membranes. In 1991 a group led by WKS was organized into the Laboratory of Structure and Dynamics of Biological Membranes, the effective member of which is AW. Using mainly the electron paramagnetic resonance (EPR) spin-labeling method, we obtained unexpected results, which are significant for the better understanding of the functioning of biological membranes. We have developed a new pulse EPR spin-labeling method for the detection of membrane domains and evaluation of lipid exchange rates. This review will be focused on our main results which can be summarized as follows: (1) Unsaturation of alkyl chains greatly reduces the ordering and rigidifying effects of cholesterol although the unsaturation alone gives only minor fluidizing effects, as observed by order and reorientational motion, and rather significant rigidifying effects, as observed by translational motion of probe molecules; (2) Fluid-phase model membranes and cell plasma membranes are not barriers to oxygen and nitric oxide transport; (3) Polar carotenoids can regulate membrane fluidity in a way similar to cholesterol; (4) Formation of effective hydrophobic barriers to the permeation of small polar molecules across membranes requires alkyl chain unsaturation and/or the presence of cholesterol; (5) Fluid-phase micro-immiscibility takes place in cis-unsaturated phosphatidylcholine-cholesterol membranes and induces the formation of cholesterol-rich domains; (6) In membranes containing high concentrations of transmembrane proteins a new lipid domain is formed, with lipids trapped within aggregates of proteins, in which the lipid dynamics is diminished to the level of gel-phase.
Źródło:
Acta Biochimica Polonica; 2000, 47, 3; 613-625
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Dynamics of neutral lipid storage in yeast.
Autorzy:
Müllner, Heidemarie
Daum, Günther
Powiązania:
https://bibliotekanauki.pl/articles/1043266.pdf
Data publikacji:
2004
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
steryl esters
triacylglycerols
lipid storage
lipid particles
yeast
Opis:
Since energy storage is a basic metabolic process, the synthesis of neutral lipids occurs in all kingdoms of life. The yeast, Saccharomyces cerevisiae, widely accepted as a model eukaryotic cell, contains two classes of neutral lipids, namely steryl esters and triacylglycerols. Triacylglycerols are synthesized through two pathways governed by the acyl-CoA diacylglycerol acyltransferase Dga1p and the phospholipid diacylglycerol acyltransferase Lro1p, respectively. Steryl esters are formed by the two steryl ester synthases Are1p and Are2p, two enzymes with overlapping function which also catalyze triacylglycerol formation, although to a minor extent. Storage of neutral lipids is tightly linked to the biogenesis of so called lipid particles. The role of this compartment in lipid homeostasis and its interplay with other organelles involved in neutral lipid dynamics, especially the endoplasmic reticulum and the plasma membrane, are subject of current investigations. In contrast to neutral lipid formation, mobilization of triacylglycerols and steryl esters in yeast are less characterized at the molecular level. Only recently, the triacylglycerol lipase Tgl3p was identified as the first yeast enzyme of this kind by function. Genes and gene products governing steryl ester mobilization still await identification. Besides biochemical properties of enzymes involved in yeast neutral lipid synthesis and degradation, regulatory aspects of these pathways and cell biological consequences of neutral lipid depletion will be discussed in this minireview.
Źródło:
Acta Biochimica Polonica; 2004, 51, 2; 323-347
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Lipid-binding proteins as stabilizers of membrane microdomains - possible physiological significance.
Autorzy:
Bandorowicz-Pikuła, Joanna
Powiązania:
https://bibliotekanauki.pl/articles/1044288.pdf
Data publikacji:
2000
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
lipid-protein interactions
lipid-binding proteins
membrane microdomains
Opis:
Below the melting point temperature of lipids, artificial lipid membranes usually exist in the ordered gel phase. Above these temperatures lipid acyl chains become fluid and disordered (liquid-crystalline phase). Depending on the chemical composition of artificial membranes, phase separation may occur, leading to the formation of transient or stable membrane domains. A similar phase separation of lipids into ordered and disordered domains has been observed in natural membranes at physiological temperature range. Moreover, it has been reported that certain proteins prefer certain organization of lipids, as for example glycosylphosphatidylinositol-anchored proteins or Src family of tyrosine kinases. The aim of present review is to discuss the possibility that some lipid microdomains are induced or stabilized by lipid-binding proteins that under certain conditions, for example due to a rise of cytosolic Ca2+ or pH changes, may attach to the membrane surface, inducing clustering of lipid molecules and creation of ordered lipid microdomains. These domains may than attract other cytosolic proteins, either enzymes or regulatory proteins. It is, therefore, postulated that lipid microdomains play important roles within a cell, in signal transduction and enzymatic catalysis, and also in various pathological states, as Alzheimer's disease, anti-phosphatidylserine syndrome, or development of multidrug resistance of cancer cells.
Źródło:
Acta Biochimica Polonica; 2000, 47, 3; 553-564
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Fatty acid composition of horse tissues depending on their position in the carcass
Autorzy:
Jankowska, B.
Korzeniowski, W.
Kwiatkowska, A.
Powiązania:
https://bibliotekanauki.pl/articles/1372711.pdf
Data publikacji:
1996
Wydawca:
Instytut Rozrodu Zwierząt i Badań Żywności Polskiej Akademii Nauk w Olsztynie
Tematy:
horse
meat technology
muscle lipid
carcass
fatty acid
reserve lipid
tissue
composition
Opis:
The purpose of this work was to compare the fatty acid composition of the following reserve lipid tissues: mane, perirenal and intermuscular, and muscles: semimembranous with thigh adductor, middle gluteal, dorsal longissimus and infraspinous with serratus. Perirenal tissue contained significantly less unsaturated fatty acids (59.96%, p<0.05) compared to mane (61.43%, p<0.05) and intermuscular (61.24%, p<0.05) tissues. The tissues differed in the content of palmitic, stearic, oleopalmitic, miristic, and linolenic acids. All the tissues had a predominance (from 2.87% to 3.92%) of linolenic acid over linoleic acid, unique to animal fats. The percentage of particular fatty acids, except palmitic and linoleic acids in all muscle lipids was very similar. In contrast to reserve lipid tissues, the content of linoleic acid in muscle lipids was higher by 1.68% to 4.18% than of linolenic acid.
Celem podjętych badań było porównanie składu kwasów tłuszczowych zapasowych tkanek Puszczowych tusz końskich; grzywowej, okołonerkowej i międzymięśniowej oraz mięśni: półbłoniastego uda z przywodzicielem uda, pośladkowego środkowego, najdłuższego grzbietu, podgrzebieniowego z zębatym brzusznym. Ustalono, że tkanka okołonerkowa zawierała istotnie mniej kwasów nienasyconych (59.96%) w porównaniu do tkanek grzywowej (61.43%) międzymięśniowej (61.24%). Badane tkanki różniły się ilością kwasów palmitynowego, stearynowego, oleopalmitynowego, mirystynowego i linolenowego. Wszystkie tkanki tłuszczowe zapasowe cechowała unikalna wśród tłuszczów zwierzęcych przewaga kwasu linolenowego nad molowym wynosząca od 2.87% do 3.92%. Lipidy wszystkich wymienionych mięśni charakteryzowały się bardzo wyrównanym udziałem procentowym poszczególnych kwasów tłuszczowych, za wyjątkiem kwasów palmitynowego i linolowego. W lipidach mięśni w przeciwieństwie do tkanek zapasowych ilość kwasu linolowego była wyższa niż kwasu linolenowego d 1.68% do 4.14%.
Źródło:
Polish Journal of Food and Nutrition Sciences; 1996, 05, 1; 41-49
1230-0322
2083-6007
Pojawia się w:
Polish Journal of Food and Nutrition Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Processes of lipids peroxidation and antioxidant activity of the oral fluid for patients with acute odontogenic osteomielitis complicated with phlegmons of different location
Autorzy:
Pohodenko-Chudakova, I. O.
Kabanova, A. A.
Powiązania:
https://bibliotekanauki.pl/articles/285451.pdf
Data publikacji:
2011
Wydawca:
Akademia Górniczo-Hutnicza im. Stanisława Staszica w Krakowie. Polskie Towarzystwo Biominerałów
Tematy:
phlegmons
lipid peroxidation
biomaterials
Źródło:
Engineering of Biomaterials; 2011, 14, no. 109-111 spec. iss.; 6-9
1429-7248
Pojawia się w:
Engineering of Biomaterials
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The reactions of hypochlorous acid, the reactive oxygen species produced by myeloperoxidase, with lipids.
Autorzy:
Spickett, Corinne
Jerlich, Andreas
Panasenko, Oleg
Arnhold, Juergen
Pitt, Andrew
Stelmaszyńska, Teresa
Schaur, R.
Powiązania:
https://bibliotekanauki.pl/articles/1044206.pdf
Data publikacji:
2000
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
lipid chlorohydrins
lipids
unsaturated fatty acids
hypochlorous acid
lipid peroxidation
low density lipoprotein
Opis:
Myeloperoxidase (MPO), an abundant enzyme in phagocytes, has been implicated in the pathogenesis of various inflammatory diseases including atherosclerosis. The major oxidant produced by MPO, hypochlorous acid (HOCl), is able to modify a great variety of biomolecules by chlorination and/or oxidation. In this paper the reactions of lipids (preferentially unsaturated fatty acids and cholesterol) with either reagent HOCl or HOCl generated by the MPO-hydrogen peroxide-chloride system are reviewed. One of the major issues has been whether the reaction of HOCl with lipids of low density lipoprotein (LDL) yields predominantly chlorohydrins or lipid hydroperoxides. Electrospray mass spectrometry provided direct evidence that chlorohydrins rather than peroxides are the major products of HOCl- or MPO-treated LDL phosphatidylcholines. Nevertheless lipid peroxidation is a possible alternative reaction of HOCl with polyunsaturated fatty acids if an additional radical source such as pre-formed lipid hydroperoxides is available. In phospholipids carrying a primary amino group such as phosphatidylethanolamine chloramines are the preferred products compared to chlorohydrins. Cholesterol can be converted by HOCl to great variety of oxysterols besides three isomers of chlorohydrins. For the situation in vivo it appears that the type of reaction occurring between HOCl and lipids would very much depend on the circumstances, e.g. the pH and the presence of radical initiators. The biological effects of lipid chlorohydrins are not yet well understood. It has been shown that chlorohydrins of both unsaturated fatty acids as well as of cholesterol may cause lysis of target cells, possibly by disruption of membrane structures.
Źródło:
Acta Biochimica Polonica; 2000, 47, 4; 889-899
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Lipid radicals: Properties and detection by spin trapping.
Autorzy:
Stolze, Klaus
Udilova, Natascha
Nohl, Hans
Powiązania:
https://bibliotekanauki.pl/articles/1044212.pdf
Data publikacji:
2000
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
DEPMPO
spin trapping
linoleic acid hydroperoxide
free radicals
lipid peroxidation
ESR
lipid radicals
Opis:
Unsaturated lipids are rapidly oxidized to toxic products such as lipid hydroperoxides, especially when transition metals such as iron or copper are present. In a Fenton-type reaction Fe2+ converts lipid hydroperoxides to the very short-lived lipid alkoxyl radicals. The reaction was started upon the addition of Fe2+ to an aqueous linoleic acid hydroperoxide (LOOH) emulsion and the spin trap in the absence of oxygen. Even when high concentrations of spin traps were added to the incubation mixture, only secondary radical adducts were detected, probably due to the rapid rearrangement of the primary alkoxyl radicals. With the commercially available nitroso spin trap MNP we observed a slightly immobilized ESR spectrum with only one hydrogen splitting, indicating the trapping of a methinyl fragment of a lipid radical. With DMPO or 5-diethoxyphosphoryl-5-methyl-1-pyrroline N-oxide (DEPMPO) adducts were detected with carbon-centered lipid radical, with acyl radical, and with the hydroxyl radical. We also synthesized lipophilic derivatives of the spin trap DEPMPO in order to detect lipid radical species generated in the lipid phase. With all spin traps studied a lipid-derived carbon-centered radical was obtained in the anaerobic incubation system Fe2+/LOOH indicating the trapping of a lipid radical, possibly generated as a secondary reaction product of the primary lipid alkoxyl radical formed. Under aerobic conditions an SOD-insensitive oxygen-centered radical adduct was formed with DEPMPO and its lipophilic derivatives. The observed ESR parameters were similar to those of alkoxyl radical adducts, which were independently synthesized in model experiments using Fe3+-catalyzed nucleophilic addition of methanol or t-butanol to the respective spin trap.
Źródło:
Acta Biochimica Polonica; 2000, 47, 4; 923-930
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Produkty utleniania lipidów jako potencjalny problem zdrowotny oraz analityczny
Lipid oxidation products as a potential health and analytical problem
Autorzy:
Dąbrowska, M.
Zielińska, A.
Nowak, I.
Powiązania:
https://bibliotekanauki.pl/articles/142838.pdf
Data publikacji:
2015
Wydawca:
Stowarzyszenie Inżynierów i Techników Przemysłu Chemicznego. Zakład Wydawniczy CHEMPRESS-SITPChem
Tematy:
utlenianie lipidów
wolne rodniki
produkty utleniania lipidów
stabilność lipidów
analiza produktów utleniania lipidów
lipid oxidation
free radicals
lipid oxidation products
lipid stability
analysis of lipid oxidation products
Opis:
Lipidy, szczególnie te nienasycone, są związkami niestabilnymi chemiczne i łatwo ulegają reakcjom oksydacji. Utlenione lipidy stanowią, trudną w analizie, mieszaninę różnych związków, które wpływają nie tylko na pogorszenie się jakości żywności, ale także prowadzą do niekorzystnych zmian w organizmie człowieka. W artykule przedstawiono ogólny mechanizm reakcji oksydacji lipidów oraz wskazano na znaczenie powstałych produktów w aspekcie żywności, surowców kosmetycznych oraz zdrowia człowieka. Omówiono także dostępne metody analizy produktów utleniania lipidów oraz związane z nią problemy.
Lipids, especially unsaturated ones, are unstable compounds which easily undergo oxidation reactions. Difficult to analyze, oxidized lipids are a mixture of different compounds which not only cause the deterioration of food quality, but also lead to adverse changes in the human body. This article presents a general mechanism of lipid oxidation reaction and shows the importance of the resulting products in terms of food, cosmetic raw materials and human health. The available analysis methods of lipid oxidation products and the associated problems are also discussed.
Źródło:
Chemik; 2015, 69, 2; 89-94
0009-2886
Pojawia się w:
Chemik
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Prognostic efficiency of inflammatory complications development of dental implantation based on indicators of lipid peroxidation (LPO) of oral fluid
Autorzy:
Pohodenko-Chudakova, I. O.
Karsyuk, Y. V.
Powiązania:
https://bibliotekanauki.pl/articles/284842.pdf
Data publikacji:
2017
Wydawca:
Akademia Górniczo-Hutnicza im. Stanisława Staszica w Krakowie. Polskie Towarzystwo Biominerałów
Tematy:
dental implants
complications
lipid peroxidation
Opis:
The frequency of complications of dental implantation at the present stage varies from 6% to 23%, what determined the relevance of this examination. The aim of this work is to determine the predictive effectiveness of the development of inflammatory complications of dental implantation on the basis of indicators of lipid peroxidation (POL) of the oral fluid (level of malondialdehyde (MDA) and the activity level of superoxide dismutase (SOD)). We examined 49 patients 25-59 years old which had no traumas, operations and diseases requiring medical rehabilitation, inflammatory disease in the maxillofacial area and the gastrointestinal tract, as well as other factors that can affect qualitative and quantitative content of the oral fluid. These patients underwent delayed dental implantation (one implant in one segment of the jaw). The POL indices were determined twice: before operation of dental implantation and 3 days after operation. The effectiveness of the proposed method was determined in accordance to the clinical and economic studies requirements. Prognostic efficiency in accordance with the procedure of conducting clinical and economic examinations on the basis of MDA level was 78%, and based on the level of SOD activity – 76%, which classifies it as high and allow to reduce the number of complications and diagnostic mistakes including in the planning and execution of invasive procedures associated with the installation of dental implants.
Źródło:
Engineering of Biomaterials; 2017, 20, no. 143 spec. iss.; 13
1429-7248
Pojawia się w:
Engineering of Biomaterials
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Arabidopsis lipocalins AtCHL and AtTIL have overlapping functions essential for lipid protection and seed longevity
Autorzy:
Simona, B.
Powiązania:
https://bibliotekanauki.pl/articles/80754.pdf
Data publikacji:
2013
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
conference
Arabidopsis
lipocalin
oxidative stress
photooxidative stress
lipid peroxidation
photosensitivity
polyunsaturated lipid
seed longevity
Źródło:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2013, 94, 2
0860-7796
Pojawia się w:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The effect of hexadecaprenol on molecular organisation and transport properties of model membranes.
Autorzy:
Janas, Teresa
Nowotarski, Krzysztof
Gruszecki, Wiesław
Janas, Tadeusz
Powiązania:
https://bibliotekanauki.pl/articles/1044307.pdf
Data publikacji:
2000
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
voltammetric studies.
bilayer lipid membranes
Langmuir film studies
long-chain polyprenol
lipid monolayer
Opis:
The Langmuir monolayer technique and voltammetric analysis were used to investigate the properties of model lipid membranes prepared from dioleoylphosphatidylcholine (DOPC), hexadecaprenol (C80), and their mixtures. Surface pressure- molecular area isotherms, current-voltage characteristics, and membrane conductance-temperature were measured. Molecular area isobars, specific molecular areas, excess free energy of mixing, collapse pressure and collapse area were determined for lipid monolayers. Membrane conductance, activation energy of ion migration across the membrane, and membrane permeability coefficient for chloride ions were determined for lipid bilayers. Hexadecaprenol decreases the activation energy and increases membrane conductance and membrane permeability coefficient. The results of monolayer and bilayer investigations show that some electrical, transport and packing properties of lipid membranes change under the influence of hexadecaprenol. The results indicate that hexadecaprenol modulates the molecular organisation of the membrane and that the specific molecular area of polyprenol molecules depends on the relative concentration of polyprenols in membranes. We suggest that hexadecaprenol modifies lipid membranes by the formation of fluid microdomains. The results also indicate that electrical transmembrane potential can accelerate the formation of pores in lipid bilayers modified by long chain polyprenols.
Źródło:
Acta Biochimica Polonica; 2000, 47, 3; 661-673
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Efekt zmiennej koncentracji fosforu na efektywność syntezy lipidów mikroglonów Chlorella vulgaris
Lipid accumulation of Chlorella vulgaris under different phosphate concentrations
Autorzy:
Rokicka, M.
Zieliński, M.
Szwarc, K.
Szwarc, D.
Dębowski, M.
Powiązania:
https://bibliotekanauki.pl/articles/400568.pdf
Data publikacji:
2017
Wydawca:
Polskie Towarzystwo Inżynierii Ekologicznej
Tematy:
mikroglony
lipidy
biodiesel
microalgae
lipid
Opis:
Hodowla i wykorzystanie mikroglonów jest obecnie intensywnie rozwijającym się obszarem badawczym. Niektóre gatunki mikroalg, w odpowiednich warunkach, gromadzą duże ilości tłuszczu w komórkach, który ma odpowiedni profil kwasów tłuszczowych do produkcji biodiesela. Etapem kierunkującym mikroglony na akumulację lipidów jest hodowla w określonych warunkach fizykochemicznych. Celem badań było określenie wpływu zmiennego stężenia fosofru w medium hodowlanym na olejogenność mikroglonów Chlorella vulgaris oraz wyznaczenie parametrów hodowli w trybie szoku. Przeprowadzone badania potwierdziły możliwość stosowania szoku fosforowego w celu makasymalizacji syntezy lipidów przez mikroglony Chlorella vulgaris. W trakcie badań uzyskano 45,23% oleju z biomasy z hodowli z szokiem fosforowym, natomiast z biomasy pochodzącej z hodowli standardowej uzyskano o 18% oleju mniej niż z biomasy pochodzącej z hodowli z deficytem fosforu.
The cultivation and utilization of microalgae is now a intensively developing area of research. Some species of microalgae, under appropriate conditions, accumulate large amounts of lipids in the cells. This lipids have a suitable profile of fatty acids for biodiesel production. The culture of microalgae for lipids accumulation should be performed in certain physicochemical conditions. The aim of the study was to determine the effect of variable ortophophates concentrations in the culture medium for lipids accumulation of microalgae Chlorella vulgaris and to determine of parameters of the phosphoric shock in the medium. The study confirmed the possibility of the use of the phosphoric shock in the medium to maximize lipids accumulation by the microalgae Chlorella vulgaris. In the study, 45.23% of the oil was obtained from the biomass from the culture with phosphoric shock in the medium and 18% less of the oil was obtained from the biomass from the standard culture.
Źródło:
Inżynieria Ekologiczna; 2017, 18, 2; 123-129
2081-139X
2392-0629
Pojawia się w:
Inżynieria Ekologiczna
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Resorcinolic lipids from cereal bran as natural antioxidants
Autorzy:
Nienartowicz, B.
Kozubek, A.
Powiązania:
https://bibliotekanauki.pl/articles/1371610.pdf
Data publikacji:
1993
Wydawca:
Instytut Rozrodu Zwierząt i Badań Żywności Polskiej Akademii Nauk w Olsztynie
Tematy:
bran milling fraction
biochemistry
cereal bran
lipid oxidation
food
natural antioxidant
cereal
antioxidant
resorcinolic lipid
Źródło:
Polish Journal of Food and Nutrition Sciences; 1993, 02, 4
1230-0322
2083-6007
Pojawia się w:
Polish Journal of Food and Nutrition Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Utilization of microorganisms for production of monoacylglycerols
Autorzy:
Bednarski, W.
Kowalewska-Piontas, J.
Adamczak, M.
Zadernowski, R.
Powiązania:
https://bibliotekanauki.pl/articles/1371615.pdf
Data publikacji:
1993
Wydawca:
Instytut Rozrodu Zwierząt i Badań Żywności Polskiej Akademii Nauk w Olsztynie
Tematy:
production
utilization
microorganism
monoacylglycerol
waste lipid
Źródło:
Polish Journal of Food and Nutrition Sciences; 1993, 02, 4; 99-103
1230-0322
2083-6007
Pojawia się w:
Polish Journal of Food and Nutrition Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Does Tofu Wastewater Conversions Nutrient Increase the Content of the Chlorella pyrenoidosa?
Autorzy:
Musa, Muhammad
Arsad, Sulastri
Sari, Luthfiana Aprilianita
Lusiana, Evellin Dewi
Kasitowati, Rarasrum Dyah
Yulinda, Elvana Nur
Nadhira, Tara
Cahyani, Devi
Powiązania:
https://bibliotekanauki.pl/articles/1839091.pdf
Data publikacji:
2021
Wydawca:
Polskie Towarzystwo Inżynierii Ekologicznej
Tematy:
carbohydrate
lipid
microalgae
organic wastewater
protein
Opis:
This study aims to analyze the lipid, carbohydrate and protein content of Chlorella pyrenoidosa after being treated with the tofu wastewater. The method used in this study was an experimental method with 4 treatments and 3 replications. The treatment was performed by administering different concentrations of the tofu wastewater to the C. pyrenoidosa. The concentrations used were 10%, 15% and 20%. Determination of these concentrations was based on the preliminary test. The main parameters observed were the lipid, carbohydrate and protein content of C. pyrenoidosa and the supporting parameters were the growth rate, doubling time and characteristics of the tofu wastewater. The study was conducted for 8 days using the batch culture method. In the exponential phase, the microalgae were harvested and then their contents were analyzed. The data obtained were analyzed using MS Office Excel 2016. The highest content of lipid, carbohydrate and protein of C. pyrenoidosa was in the treatment of 20% tofu wastewater, reaching 1.56%; 28.92%; and 28.92%, respectively. Meanwhile, the highest growth rate and the smallest doubling time in the treatment of 20% tofu wastewater accounted for 0.8264 day-1 and 0.0349 hours-1. Moreover, the rates of BOD and TSS of the tofu wastewater at the end of the study decreased. It can be concluded that administering different concentrations of the tofu wastewater can affect the lipid, carbohydrate and protein content of C. pyrenoidosa. It was suggested that further research is needed to conduct semi-continuous cultivation of C. pyrenoidosa using a higher density so that the results obtained can be optimized.
Źródło:
Journal of Ecological Engineering; 2021, 22, 2; 70-76
2299-8993
Pojawia się w:
Journal of Ecological Engineering
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Influence of lipid stabilization on the retention of available lysine and methionine in stored raw Polish sausage
Wpływ stabilizowania lipidów na zachowanie dostępnej lizyny i metioniny w przechowywanej kiełbasie typu "polska surowa"
Autorzy:
Hes, M.
Korczak, J.
Pyrcz, J.
Kowalski, R.
Powiązania:
https://bibliotekanauki.pl/articles/826678.pdf
Data publikacji:
2009
Wydawca:
Polskie Towarzystwo Technologów Żywności
Tematy:
Polish sausage
lipid oxidation
lipid stabilization
raw sausage
sausage
storage
available lysine
lysine
methionine
antioxidant
Opis:
The aim of the study was to determine the effect of the addition of antioxidants on changes in contents of available lysine and methionine in raw Polish sausage. Experimental material consisted of four variants of experimental sausages. One variant consisted of a sample with no antioxidant added (control), while the following were added to the other variants: BHT (0.02%), rosemary ethanol extract (0.05%) and soy protein hydrolysate (2%). Oxidation degree of lipid using peroxide value (PV), anisidine value (AV), thiobarbituric acid reactive substances (TBARS) and the Totox index was controlled and content of available lysine and methionine were determined periodically. In order to determine the effect of the addition of antioxidants on the stability of available lysine and methionine, values of the coefficient of the slope of curve of changes in time (coefficient a/24 h) were analyzed along with the half-life period of the amino acids (TIC50). Antioxidants exhibited an inhibitory effect on the advancement of lipid autoxidation reactions and reduced quantitative losses of available lysine and methionine in analyzed sausages. Natural antioxidants exhibited a lower capacity to reduce losses of nutritive value of protein than it was found for BHT.
W pracy określono wpływu dodatku przeciwutleniaczy na stabilność dostępnej lizyny i metioniny w kiełbasie typu „polska surowa”, jako istotnych czynników determinujących wartość żywieniową produktów mięsnych. Materiałem badawczym były cztery warianty wędlin doświadczalnych. Jeden wariant stanowiła próba bez dodatku przeciwutleniacza (próba kontrolna), do pozostałych dodano odpowiednio: BHT (0,02%), etanolowy ekstrakt rozmarynu (0,05%) i hydrolizat sojowy (2%). Okresowo oznaczano liczbę nadtlenkową (PV), liczbę anizydynową (AV), zawartość substancji dających reakcję barwną z kwasem 2- tiobarbiturowym (TBARS), indeks Totox oraz zawartość dostępnej lizyny i metioniny. W celu określenia aktywności przeciwutleniającej badanych dodatków wyznaczono współczynniki kierunkowe (Wsp.a/24 h), stanowiące kąt nachylenia krzywej regresji wykreślonej z punktów pomiarowych danego wskaźnika w czasie. Wpływ przeciwutleniaczy na stabilność dostępnej lizyny i metioniny analizowano na podstawie wyznaczonego czasu połowicznego rozpadu aminokwasów (TIC50). Przeciwutleniacze wykazywały hamujący wpływ na postęp reakcji autooksydacji lipidów w badanych wędlinach oraz w istotny sposób zmniejszały straty ilościowe dostępnej lizyny i metioniny. Przeciwutleniacze naturalne wykazywały mniejszą zdolność obniżania strat wartości odżywczej białka niż BHT.
Źródło:
Żywność Nauka Technologia Jakość; 2009, 16, 4
1425-6959
Pojawia się w:
Żywność Nauka Technologia Jakość
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Exceptional molecular organization of canthaxanthin in lipid membranes
Autorzy:
Sujak, Agnieszka
Powiązania:
https://bibliotekanauki.pl/articles/1039765.pdf
Data publikacji:
2012
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
molecular interactions
retinopathy
lipid membranes
canthaxanthin
Opis:
Canthaxanthin (β,β-carotene 4,4' dione) used widely as a drug or as a food and cosmetic colorant may have some undesirable effects on human health, caused mainly by the formation of crystals in the macula lutea membranes of the retina of an eye. Experiments show the exceptional molecular organization of canthaxanthin and a strong effect of this pigment on the physical properties of lipid membranes. The most striking difference between canthaxanthin and other macular pigments is that the effects of canthaxanthin at a molecular level are observed at much lower concentration of this pigment with respect to lipid (as low as 0.05 mol%). An analysis of the molecular interactions of canthaxanthin showed molecular mechanisms such as: strong van der Waals interactions between the canthaxanthin molecule and the acyl chains of lipids, restrictions to the segmental molecular motion of lipid molecules, modifications of the surface of the lipid membranes, effect on the membrane thermotropic properties and finally interactions based on the formation of the hydrogen bonds. Such interactions can lead to a destabilization of the membrane and loss of membrane compactness. In the case of the retinal vasculature, it can lead to an increase in the permeability of the retinal capillary walls and the development of retinopathy.
Źródło:
Acta Biochimica Polonica; 2012, 59, 1; 31-33
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Species- and substrate-specific stimulation of human plasma paraoxonase 1 (PON1) activity by high chloride concentration.
Autorzy:
Bełtowski, Jerzy
Wójcicka, Grażyna
Marciniak, Andrzej
Powiązania:
https://bibliotekanauki.pl/articles/1043697.pdf
Data publikacji:
2002
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
paraoxonase
arylesterase
lipid peroxidation
oxidative stress
Opis:
Paraoxonase 1 (PON1), contained in plasma high-density lipoproteins, plays an important role in the protection of plasma lipoproteins and cell membranes from oxidative damage. Previous studies indicate that human PON1 is stimulated by high NaCl concentrations. The aim of this study was to characterize in more detail the effect of salts on serum PON1. Paraoxon-hydrolyzing activity of human serum was stimulated by 81.6% following the addition of 1 M NaCl. The effect of NaCl was dose-dependent between 0.5 and 2 M. PON1 activity toward phenyl acetate was reduced by 1 M NaCl by 55.2%. Both the paraoxon- and phenyl acetate-hydrolysing activity was slightly lower in heparinized plasma than in serum, but NaCl had similar stimulatory and inhibitory effects on these activities, respectively. In rat, rabbit, and mouse, NaCl reduced PON1 activity. KCl had a similar effect on human PON1 as NaCl. Sodium nitrite also stimulated human PON1 but much less effectively than chloride salts. In contrast, sucrose, sodium acetate and sodium lactate had no significant effect. NaBr was a less effective PON1 activator than NaCl, whereas the effect of NaJ was non-significant. The activity of human PON1 toward homogentisic acid lactone and γ-decanolactone was unaltered by NaCl. These data indicate that: 1) high concentrations of chlorides stimulate human PON1 activity toward paraoxon but not other substrates, 2) PON1 is inhibited by Cl- in other mammalian species, 3) the potency of human PON1 activation by halogene salts increases with decreasing atomic mass of the halide anion.
Źródło:
Acta Biochimica Polonica; 2002, 49, 4; 927-936
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The effect of sauna bathing on lipid profile in young, physically active, male subjects
Autorzy:
Gryka, Dorota
Pilch, Wanda
Szarek, Marta
Szygula, Zbigniew
Tota, Łukasz
Powiązania:
https://bibliotekanauki.pl/articles/2178864.pdf
Data publikacji:
2014-08-06
Wydawca:
Instytut Medycyny Pracy im. prof. dra Jerzego Nofera w Łodzi
Tematy:
human lipid profile
sauna
plasma volume
Opis:
Objectives: The aim of the study was to evaluate effects of Finnish sauna bathing on lipid profile in healthy, young men. Material and Methods: Sixteen male subjects (20–23 years) were subjected to 10 sauna bathing sessions in a Finnish sauna every 1 or 2 days. The mean sauna temperature was 90±2°C, while humidity was 5–16%. Each session consisted of three 15-minute parts and a 2-minute cool-down between them. The following measurements were taken before and after the sauna sessions: body mass, heart rate, body skinfold thickness. The percentage fat content and then, the lean body mass were calculated. Total cholesterol, triacylglycerols, lipoprotein cholesterol LDL and HDL were measured in blood samples. Results: A statistically significant decrease of total cholesterol and LDL cholesterol was observed during 3 weeks of sauna treatment and in the week afterwards. A significant decline in triacylglycerols was found directly after the 1st and 24 h directly after the 10th sauna session. After the 10th sauna session the level of HDL cholesterol remained slightly increased, but this change was not statistically significant. A decrease in blood plasma volume was found directly after the 1st and the last sauna bathing session due to perspiration. An adaptive increase in blood plasma volume was also found after the series of 10 sauna sessions. Conclusions: Ten complete sauna bathing sessions in a Finnish sauna caused a reduction in total cholesterol and LDL cholesterol fraction levels during the sessions and a gradual return of these levels to the initial level during the 1st and the 2nd week after the experiment. A small, statistically insignificant increase in HDL-C level and a transient decline in triacylglycerols were observed after those sauna sessions. The positive effect of sauna on lipid profile is similar to the effect that can be obtained through a moderate-intensity physical exercise.
Źródło:
International Journal of Occupational Medicine and Environmental Health; 2014, 27, 4; 608-618
1232-1087
1896-494X
Pojawia się w:
International Journal of Occupational Medicine and Environmental Health
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Changes in content of polar and non-polar lipids in plants with different chilling sensitivity
Autorzy:
Wojtowicz, J.
Gieczewska, K.
Garstka, M.
Powiązania:
https://bibliotekanauki.pl/articles/80143.pdf
Data publikacji:
2013
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
conference
polar lipid
non-polar lipid
content change
chilling stress
necrotic spot
chloroplast
plant cell
thylakoid membrane
Źródło:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2013, 94, 3
0860-7796
Pojawia się w:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Optimization of flotation assay conditions for syndapin binding to phosphatidic acid containing liposomes
Autorzy:
Piaścik, Magda
Zegarlińska, Jolanta
Sikorski, Aleksander F.
Czogalla, Aleksander
Powiązania:
https://bibliotekanauki.pl/articles/764838.pdf
Data publikacji:
2017
Wydawca:
Uniwersytet Łódzki. Wydawnictwo Uniwersytetu Łódzkiego
Tematy:
protein-lipid interactions
LUVs
density gradient
ultracentrifugation
Opis:
Flotacja jest jedną z najefektywniejszych metod wstępnej identyfikacji oddziaływań białko-błony lipidowe. W większości przypadków wykorzystuje się w niej małe jednowarstwowe pęcherzyki lipidowe, które służą jako modele błonowe i nie wymagają dodatkowych nośników, takich jak membrany czy nanocząstki polimerowe, które są często używane w innych metodach mających na celu identyfikację oddziaływań białkolipid. W poniższej pracy prezentujemy wyniki uzyskane podczas badań oddziaływań kwasu fosfatydowego i syndapiny. Omawiamy także niektóre techniczne aspekty metody, kładąc nacisk na to jak małe zmiany w warunkach metody mogą wpłynąć na otrzymywane wyniki.
Flotation is one of the best method for preliminary identification of protein-lipid interactions. In most widely used approach it utilizes large unilamellar vesicles, that are excellent models of freestanding membranes and do not require any additional components, like solid supports or beads that are needed in other methods commonly used for protein-lipid binding studies. Here we present results obtained during our studies on phosphatidic acid - syndapin interactions and discuss some technical aspects of this method underlying how relatively small changes in the conditions can influence the results.
Źródło:
Acta Universitatis Lodziensis. Folia Biologica et Oecologica; 2017, 13; 9-17
1730-2366
2083-8484
Pojawia się w:
Acta Universitatis Lodziensis. Folia Biologica et Oecologica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Straight and branched (ω-1)-hydroxylated very long chain fatty acids are components of Bradyrhizobium lipid A
Autorzy:
Choma, Adam
Komaniecka, Iwona
Powiązania:
https://bibliotekanauki.pl/articles/1039947.pdf
Data publikacji:
2011
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
symbiosis
fatty acids
LPS
Bradyrhizobium
lipid A
Opis:
Lipopolysaccharides of seven Bradyrhizobium strains and three whole-cell fatty acid preparations from bacteria isolated from nodules of Sarothamnus scoparius (Common Broom) were studied for the presence of very long chain (ω-1)-hydroxy fatty acids. Several such fatty acids were identified. Among them, straight-chain as well as mono- and dimethyl branched acids with chains in the range from 26 to 34 carbon atoms were found. Pyrrolidides and 4,4-dimethyloxazoline derivatives were used to determine the branching position. Carbons at the (ω-10) and/or (ω-11) positions in alkyl chains were points of attachment of methyl groups. These data complete the structure of bradyrhizobial lipid A with important details. The obtained results can be applied in the chemotaxonomy of Bradyrhizobium.
Źródło:
Acta Biochimica Polonica; 2011, 58, 1; 51-58
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The application of melanin modified gelatin coatings for packaging and the oxidative stability of pork lard
Autorzy:
Łopusiewicz, Łukasz
Jędra, Filip
Bartkowiak, Artur
Powiązania:
https://bibliotekanauki.pl/articles/1177712.pdf
Data publikacji:
2018
Wydawca:
Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
Tematy:
antioxidant
gelatin
lard
lipid oxidation
melanin
packaging
Opis:
The influence of gelatin coatings modified with fungal melanin on pork lard oxidative stability was studied. The lard was coated with gelatin coatings containing 0.1%; 0.5%; 1% of fungal melanin and control gelatin coating devoid of melanin. The peroxide values (POV), iodine values (IV) and acid values (AV) were studied after 7, 14 and 21 days storage in controlled conditions. Lard covered with modified coatings had lowered oxidative rancidity. Hence, modified coatings containing fungal melanin can be use effectively for the prevention of lard oxidation.
Źródło:
World Scientific News; 2018, 101; 108-119
2392-2192
Pojawia się w:
World Scientific News
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Transmembrane segment M2 of glycine receptor as a model system for the pore-forming structure of ion channels.
Autorzy:
Bednarczyk, Piotr
Szewczyk, Adam
Dołowy, Krzysztof
Powiązania:
https://bibliotekanauki.pl/articles/1043689.pdf
Data publikacji:
2002
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
black lipid membrane
ion channels
glycine receptor
Opis:
The glycine receptor belongs to the ligand-gated ion channel superfamily. It is a chloride conducting channel composed of four transmembrane domains. It was previously shown that the second transmembrane domain (M2) of the glycine receptor forms an ion conduction pathway throught lipid bilayers. The amino-acid sequence of the transmembrane segment M2 of the glycine receptor has a high homology to all receptors of the ligand-gated ion channel superfamily. In our report, we have used a synthetic M2 peptide. It was incorporated into a planar membrane of known lipid composition and currents induced by M2 were measured by the Black Lipid Membrane technique. When the planar lipid bilayer was composed of 75% phosphatidylethanolamine and 25% phosphatidylserine, the reversal potential measured in a 150/600 mM KCl (cis/trans) gradient was -19 mV suggesting that the examined pore was preferential to anions, PK/PCl = 0.25. In contrast, when 75% phosphatidylserine and 25% phosphatidylethanolamine was used, the reversal potential was +20 mV and the pore was preferential to cations, PK/PCl = 4.36. Single-channel currents were recorded with two predominant amplitudes corresponding to the main-conductance and sub-conductance states. Both conductance states (about 12 pS and 30 pS) were measured in a symmetric solution of 50 mM KCl. The observed single-channel properties suggest that the selectivity and conductance of the pore formed by the M2 peptide of the glycine receptor depend on the lipid composition of the planar bilayer.
Źródło:
Acta Biochimica Polonica; 2002, 49, 4; 869-875
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Effect of metal ions from Ginkgo biloba extracts on the oxidative stability of rapeseed oil and its triacylglycerols
Autorzy:
Kobus-Cisowska, J.
Dziedzinski, M.
Szczepaniak, O.
Flaczyk, E.
Telichowska, A.
Krol, E.
Staniek, H.
Byczkiewicz, S.
Powiązania:
https://bibliotekanauki.pl/articles/1192072.pdf
Data publikacji:
2020
Wydawca:
Uniwersytet Warmińsko-Mazurski w Olsztynie / Polskie Towarzystwo Magnezologiczne im. Prof. Juliana Aleksandrowicza
Tematy:
Ginkgo biloba
minerals
lipid
oil
antioxidants
triacylglycerol
Opis:
Total antixodative effect is difficult to predict because the total antiradical capacity depends not only on properties of individual compounds, but also on their mutual interactions. The study examined the effect of the addition of Ginkgo biloba antioxidants to refined rapeseed oil on the stabilization of the oil and its triacylglycerols, measured by an Oxidograph accelerated oxidative stability test and the Rancimat test. The mineral composition of oil was also determined. The tested samples were observed to respond differently in terms of the induction period, which was the longest periods for ethanolic extracts, and the shortest for aqueoeus ones. The highest values of the protective factor were observed in the samples with added ethanolic extract of green leaves, while the lowest ones were noted in all aqueous extracts. In all the tested samples, values of the protection factor increased along with the growing extract concentration in a sample. Among the tested additives, butylated hydroxytoluene added to a sample of oil resulted in the longest induction period. Among the analysed microelements, iron was the prevalent one, with its concentration being significantly higher in water extracts. The least abundant was selenium. The antioxidative capacity of Ginkgo biloba extract resulted from the presence of transitional metals and polyphenols, as the presence of zinc, copper and iron affects the bioavailability of flavonoids. Negative relationships were noted between microelements in the extracts and its oxidative stability, which indicated the oxidative activity of the former. G. biloba extracts may be a new source of stabilising additives for high-fat foods. However, further reasearch I needed to confirm this assumption, particularly because food is a complex matrix, in which ingredients might interact with one another, causing higher or lower antioxidative capacity
Źródło:
Journal of Elementology; 2020, 25, 3; 851-862
1644-2296
Pojawia się w:
Journal of Elementology
Dostawca treści:
Biblioteka Nauki
Artykuł
Wyświetlanie 1-25 z 253

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