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    Wyświetlanie 1-41 z 41
    Tytuł:
    Primary breast angiosarcoma – a case report
    Autorzy:
    Ostańska, Elżbieta
    Aebisher, David
    Kaznowska, Ewa
    Bartusik-Aebisher, Dorota
    Powiązania:
    https://bibliotekanauki.pl/articles/2040145.pdf
    Data publikacji:
    2020-12-30
    Wydawca:
    Uniwersytet Rzeszowski. Wydawnictwo Uniwersytetu Rzeszowskiego
    Tematy:
    breast biopsy histopathological examination
    immunohistochemistry
    Opis:
    Introduction. Angiosarcoma is a rare breast cancer that can be primary or secondary after surgery or after breast cancer radiotherapy. It is important that breast angiosarcoma belongs to tumors with a non-specific clinical and radiological picture. Aim. The study of the biopsies contained aggressive vasomotor hyperplasia. Description of the case. The presented case concerns the primary angiosarcoma of the right breast in a 56-year-old woman who had never had a surgical procedure before, nor radiotherapy in the area of the breast. Conclusion. Histopathological examination supported by immunohistochemistry is a reliable and indispensable diagnostic element in the diagnosis of vascular sarcoma.
    Źródło:
    European Journal of Clinical and Experimental Medicine; 2020, 4; 331-334
    2544-2406
    2544-1361
    Pojawia się w:
    European Journal of Clinical and Experimental Medicine
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Epidemiological profile and distribution of prognostic factors in invasive breast cancer among Algerian women
    Autorzy:
    Elbasyouni, Amel
    Saadi, Leila
    Baha, AbdelKarim
    Powiązania:
    https://bibliotekanauki.pl/articles/2047226.pdf
    Data publikacji:
    2021-09-22
    Wydawca:
    Medical Education
    Tematy:
    breast cancer
    epidemiology
    biomarkers
    immunohistochemistry
    prognostic
    Opis:
    Although the widespread of early screening and advanced medical therapies, the breast cancer incidence rate continues to rise among Algerian women. This retrospective study investigated mammary lesions’ epidemiological profile and histopathological characteristics and evaluated primary invasive breast cancer prognostic factors. We found that the incidence of breast cancer increases in middle- aged women between 40 and 60 years. Scarff Bloom Richardson grade II predominates in invasive breast cancer samples. In this study, molecular profiling shows that 82.1% of invasive tumours are hormone receptor-positive. A significant correlation is observed between the age of the patient and the SBR grade (p = 0.001) and with the hormone receptor expression (p = 0.001). In addition, the tumour grade is significantly correlated to oestrogen and progesterone receptor expression (p = 0.000; p = 0.000, respectively). Twenty-two per cent of cases were human epidermal growth factor receptor 2-positive. The Ki-67 proliferation index is expressed in 91% of breast cancer patients and was significantly associated with Scarff Bloom Richardson grade (p = 0.030), the progesterone receptor expression (p = 0.029) and with human epidermal growth factor receptor 2-positivity (p = 0.023). Primary breast cancer with a high grade is more frequent (31%) in young women under 40 years old, presenting 17% of our population. In summary, breast cancer patients in Algeria develop an unfavourable profile. Immunohistochemistry assay has played a pivotal role in assessing breast cancer predictive biomarkers improving the tumour behaviour and response to treatment.
    Źródło:
    OncoReview; 2021, 11, 4; 95-101
    2450-6125
    Pojawia się w:
    OncoReview
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    The inflammatory reaction and the expression of pro- and anti-inflammatory cytokines to a novel polyester-polyurethane aortic prosthesis evaluated in dog sat 6 and 12 months post-implantation
    Autorzy:
    Mālniece, A.
    Auzāns, A.
    Powiązania:
    https://bibliotekanauki.pl/articles/2087649.pdf
    Data publikacji:
    2018
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    polyester
    polyurethane
    inflammation
    immunohistochemistry
    aortic prosthesis
    Źródło:
    Polish Journal of Veterinary Sciences; 2018, 21, 4; 655-664
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Protective effectiveness of anise against testicular ischemia and reperfusion injury: An experimental study in rats
    Autorzy:
    Yildizhan, E.
    Akkus, M.
    Ulger, B.V.
    Asır, F.
    Söker, S.
    Gündüz, E.
    Rençber, M.
    Barçin, M.
    Powiązania:
    https://bibliotekanauki.pl/articles/16647584.pdf
    Data publikacji:
    2023
    Wydawca:
    Polska Akademia Nauk. Czasopisma i Monografie PAN
    Tematy:
    Anise
    testicle
    ischemia
    reperfusion
    immunohistochemistry
    Opis:
    Testicular torsion is a frequently encountered clinical condition that requires urgent treatment. The aim of this study is to investigate the efficacy of Anise (Pimpinella anisum L.) in treating the pathological condition due to ischemia and reperfusion injury by using biochemical, histopathological and immunohistochemical methods. A total of 6 groups were formed with 8 male Wistar Albino rats in each group. Group 1 (n=8): control group, Group 2 (n=8): Anise aqueous solution was given orally 5 ml/kg by gavage for 30 days. Group 3 (n=8): Ischemia and Reperfusion (I/R) group, bilateral testicles were rotated 270° and reperfused after 30 minutes of ischemia. Group 4 (n=8): I/R+ Anise group, Group 5 (n=8): Anise+ I/R group and Group 6 (n=8): Anise+ I/R+ Anise group. The results of the Anise group and the Control group were similar. However, the damage in the I/R group was considerably more severe than in any of the other study groups. While it was observed that spermatogenic cells started to regenerate in the I/R+Anise group, edema and congestion were observed in the Anise+I/R group. In the Anise+I/R+Anise group, all histological findings and biochemical parameters were similar to those of the control group. It was observed that anise had protective effects in ischemia and reperfusion injury in rat testicles.
    Źródło:
    Polish Journal of Veterinary Sciences; 2023, 26, 2; 249-255
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Immunohistochemical evaluation of the influence of L-arginine on biomarkers of environmental (cellular) stress in the kidneys of pregnant female rats
    Autorzy:
    Pedrycz, Agnieszka
    Siermontowski, Piotr
    Powiązania:
    https://bibliotekanauki.pl/articles/989964.pdf
    Data publikacji:
    2017
    Wydawca:
    Instytut Medycyny Wsi
    Tematy:
    nitric oxide
    cellular stress
    kidneys
    pregnancy
    immunohistochemistry
    Opis:
    Introduction. Kidney damage during pregnancy constitutes a diagnostic and therapeutic challenge. However, it is not entirely known whether a kidney condition recognised before the pregnancy releases an organism’s response to pregnancy, or whether pregnancy itself worsens kidney function. Objective. The aim of the study was immunohistochemical evaluation of cells of kidneys of pregnant rats under the influence of nitric oxide (NO), with measurement of the immunoexpression of cellular stress markers (p-53, HSP 70). The dose of administered L-arginine (NO substrate) was approximated to that applied in obstetrics in gestosis prevention and treatment in pregnant women. Materials and method. 60 female rats used in experiment were divided into 6 groups: 3 experimental and 3 control. The females from experimental groups were administered L-arginine (40g/kg, per os) every other day starting from the seventh day or pregnancy. The animals were decapitated on the 10th, 20th day of pregnancy, and 10 days after the delivery. Kidneys taken from decapitated rats were evaluated using the immunohistochemical three step method. HSP 70 and p-53 proteins were detected. Conclusions. L-arginine increased the expression of p-53 protein – on the 10th day of pregnancy, which increased at the end of pregnancy; however, 10 days after delivery the level dropped below that observable during physiological pregnancy. Hormonal changes in physiological pregnancy cause an increase in expression of the p-53 (cell stress marker) in the epithelial cells of renal tubules, mainly at the end of pregnancy (20th day). 10 days after the delivery, this expression decreases. The expression of HSP-70 protein increases already on the 10th day of pregnancy and maintains a similar level throughout the pregnancy, but is reduced after the puerperium.
    Źródło:
    Annals of Agricultural and Environmental Medicine; 2017, 24, 1
    1232-1966
    Pojawia się w:
    Annals of Agricultural and Environmental Medicine
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Significance of mucin expression in pancreatic intraepithelial neoplasia (PanIN) – precursor lesions of pancreatic ductal adenocarcinoma (PDAC)
    Autorzy:
    Zińczuk, J.
    Zaręba, K.
    Pryczynicz, A.
    Kuczyńska, P.
    Boroń, Z.
    Ustymowicz, W.
    Maciorkowska, M.
    Ćwiklińska-Dworakowska, M.
    Baszun, M.
    Jelski, S.
    Guzińska-Ustymowicz, K.
    Powiązania:
    https://bibliotekanauki.pl/articles/1918638.pdf
    Data publikacji:
    2018-06-18
    Wydawca:
    Uniwersytet Medyczny w Białymstoku
    Tematy:
    Mucin
    immunohistochemistry
    pancreatic intraepithelial neoplasia
    PanIN
    Opis:
    Purpose: To evaluate chosen mucins (mucin 1, 4, 5AC) expression in pancreatic intraepithelial neoplasia, which is a precursor lesion of pancreatic ductal adenocarcinoma. Materials and methods: The study group included 70 patients operated on due to inflammation, cysts and pancreatic ductal adenocarcinoma with pancreatic intraepithelial neoplasia revealed additionally. Mucin 1, 4 and 5AC expression was assessed by immunohistochemical method using polyclonal antibodies. Results: Statistical analysis proved a positive correlation between the expression of mucin 1, 4 and 5AC proteins and the presence and staging of pancreatic intraepithelial neoplasia (p<0.001). Statistically significant correlations were determined between mucin 1, 4 and 5AC and the location of PanIN lesion in the pancreas. Positive correlations were found between mucin 5AC expression and the type of a basic disease (p=0.014). Differences in the expression of MUC 1, 4 and 5AC proteins between healthy pancreatic ducts and various stages of pancreatic intraepithelial neoplasia were statistically significant (p<0.001). Conclusions: Overexpression of mucin 1, 4 and 5AC is related to the presence of pancreatic intraepithelial neoplasia. This suggests that overproduction of mucus is a phenomenon occurring early in the process of carcinogenesis in the pancreas and has its beginning in precancerous lesions of an early stage.
    Źródło:
    Progress in Health Sciences; 2018, 8(1); 63-73
    2083-1617
    Pojawia się w:
    Progress in Health Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Horners syndrome during spindle cell lipoma of theneck–a case report
    Autorzy:
    Kaczmarczyk, Dariusz
    Kubiak, Marcin
    Komoń-Kotecka, Ewa
    Bartoszewicz, Robert
    Bruzgielewicz, Antoni
    Powiązania:
    https://bibliotekanauki.pl/articles/1401930.pdf
    Data publikacji:
    2014
    Wydawca:
    Index Copernicus International
    Tematy:
    Spindle cell lipoma
    Immunohistochemistry
    Horner's syndrome
    Opis:
    Horner's syndrome is most often caused by a tumour compressing the sympathetictrunk. Spindle cell lipoma (SCL) is a rare tumour, corresponding to just 1.5% of the benigntumours of soft tissues. Its most common localisation is subcutaneous tissue in theupper part of the body. This paper presents a rare case of Horner's syndrome caused bya SCL of the neck.A 42-year-old male patient was admitted to the ENT Ward of the Mazovian SpecialHospital in Siedlce because of a neck tumour on the left side. Laryngological examinationrevealed only a hard tumour with a limited mobility on the left side of the neck. Oph-thalmological and neurological consultations indicated Horner's syndrome. Fine-needleaspiration biopsy gave no clear diagnosis. The patient was qualified for surgery. Thetumour was radically excised and sent for histopathological examination. Thefinal his-topathological diagnosis was: SCL. Symptoms of the Horner's syndrome disappearedwithin 3 weeks after the surgery.The discussed case should be noted because of untypical SCL localisation within thecervical perivascular space, and also because of presence of symptoms of Horner's synd-rome caused by compression of the tumour on the sympathetic trunk that disappearedfollowing the surgical treatment.
    Źródło:
    Polski Przegląd Otorynolaryngologiczny; 2014, 3, 4; 222-225
    2084-5308
    2300-7338
    Pojawia się w:
    Polski Przegląd Otorynolaryngologiczny
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Immunohistochemical study on the development of cholinergic and nitrergic nerve structures in the bovine esophageal groove
    Autorzy:
    Marszałek, M.
    Serzysko, T.
    Sienkiewicz, W.
    Powiązania:
    https://bibliotekanauki.pl/articles/16539106.pdf
    Data publikacji:
    2022
    Wydawca:
    Polska Akademia Nauk. Czasopisma i Monografie PAN
    Tematy:
    esophageal groove
    acetylcholine
    nitric oxide
    immunohistochemistry
    development
    Opis:
    The proper functioning of the perinatal sucking reflex in calves is essential for the prevention of milk leakage into the rumen. The complex process behind its regulation is mediated at the gut level via multiple excitatory and inhibitory neurotransmitters, of which acetylcholine and nitric oxide are of fundamental importance. The aim of our study was to depict age-related alterations in the cholinergic and nitrergic innervation of the esophageal groove (EG) using immunohistochemistry and Real-Time PCR methods. We found out that the highest number of cholinergic nerve cells was present in the second trimester fetuses. From this developmental stage onward, their amount was gradually decreasing and reached the lowest value in 4-year-old cows. The same developmental pattern was observed for nitrergic nerve structures with the highest percentage of nitrergic neurons in the third trimester fetuses. Our observations prove that both neuronal populations are crucial for a proper closure of EG in calves. Therefore, their contribution to a general neuronal activity in the ENS diminishes with age as the high motility of a gastric groove is not necessarily required in older cattle.
    Źródło:
    Polish Journal of Veterinary Sciences; 2022, 25, 1; 165-174
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Expression of follicle-stimulating hormone receptor (FSHR), protein kinase B-2 (AKT2) and adapter protein with PH domain, PTB domain, and leucine zipper (APPL1) in pig ovaries
    Autorzy:
    Ning Zhou
    Ning Wang
    Xiang Qin
    Qian Liu
    Juhua Wang
    Hui Dong
    Jie Zhou
    Fugui Fang
    Powiązania:
    https://bibliotekanauki.pl/articles/2087861.pdf
    Data publikacji:
    2017
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    pigs
    immunohistochemistry
    FSHR
    AKT2
    APPL1
    Źródło:
    Polish Journal of Veterinary Sciences; 2017, 4; 661-667
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    High expression of CD133 – stem cell marker for prediction of clinically agressive type of colorectal cancer
    Autorzy:
    Kostovski, Ognen
    Antovic, Svetozar
    Trajkovski, Gjorgji
    Kostovska, Irena
    Jovanovic, Rubens
    Jankulovski, Nikola
    Powiązania:
    https://bibliotekanauki.pl/articles/1391698.pdf
    Data publikacji:
    2020
    Wydawca:
    Index Copernicus International
    Tematy:
    CD133
    colorectal cancer
    immunohistochemistry
    stem cells
    Opis:
    Background: Colorectal cancer (CRC) is one of the most common malignancies in the world. The cancer stem cell (CSC) markers are associated with aggressive cancer types and poor prognosis. The objective of the study was to evaluate the CD133 expression and to correlate it with clinicopathological features in patients with CRC. Material and Methods: Our study included ninety patients with CRC who underwent curative surgical resection from 2012 to 2017 at the University Clinic for Digestive Surgery, Skopje, North Macedonia. Tumor samples were first analyzed with standard histopathological methods and then the CD133 expression was investigated immunohistochemically. The level of expression of CD133 was classified semiquantitatively. Low positivity was defined as positive immunoreactivity in <50% of tumor glands, and high positivity was defined as positive immunoreactivity in ≥50% of tumor glands. Furthermore, clinicopathological features of patients were retrospectively reviewed. Results: High expression of CD133 was found in 47.8% of patients’ CRC samples. In 69.6% of patients with metastatic lesions in visceral organs we found high expression of CD133. We found statistically significant differences in the expression of CD133 between patients with and without visceral metastatic lesions (P = 0.0153), between patients with a different T category (P = 0.0119), N status (P = 0.0066) and grade (G) (P = 0.0115). Our results showed that the stage of disease has the greatest impact on expression of CD133 (P < 0.00001). Conclusion: High expression of CD133 is a useful marker for prediction of the clinically aggressive type of CRC and can be routinely implemented in standard pathohistological diagnostics.
    Źródło:
    Polish Journal of Surgery; 2020, 92, 3; 9-14
    0032-373X
    2299-2847
    Pojawia się w:
    Polish Journal of Surgery
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Immunohistochemical characterization of nerve elements in porcine intrinsic laryngeal ganglia
    Autorzy:
    Sienkiewicz, W.
    Klimczuk, M.
    Gulbinowicz-Gowkielewicz, M.
    Lepiarczyk, E.
    Kaleczyc, J.
    Powiązania:
    https://bibliotekanauki.pl/articles/16539221.pdf
    Data publikacji:
    2022
    Wydawca:
    Polska Akademia Nauk. Czasopisma i Monografie PAN
    Tematy:
    larynx
    intrinsic neurons
    pig
    immunohistochemistry
    biologically active substances
    Opis:
    The present study investigated the chemical coding of neurons and nerve fibres in local laryngeal ganglia in pigs (n=5) using double-labelling immunohistochemistry. Virtually all the neurons were cholinergic in nature (ChAT- or VAChT-positive). Only very solitary, small nerve cells (presumably representing interneurons) stained intensely for adrenergic marker, DβH. Many neurons also contained immunoreactivity for NOS (91%), VIP (62.7%), NPY (24.7%), galanin (10%), SP (1.3%) and CGRP (5.3%). No neurons expressing somatostatin or Leu-enkephalin were observed. Nearly all the neuronal somata were densely supplied with varicose cholinergic nerve terminals, which presumably represented preganglionic axons, and some of them were also closely apposed with CGRP- and/or SP-positive varicose nerve endings, which were putative collaterals of extrinsic primary sensory fibres. In conclusion, this study has revealed that intrinsic neurons in the porcine larynx, like in many other mammalian species studied, should be classified as parasympathetic cholinergic neurons expressing biologically active substances, predominantly NOS and VIP. Furthermore, they are likely to receive inputs from not only preganglionic neurons but also primary sensory nerve cells. Finally, it appears that the information on the occurrence of the local laryngeal ganglia should be regularly included in textbooks dealing with the cranial portion of the parasympathetic nervous system in mammals.
    Źródło:
    Polish Journal of Veterinary Sciences; 2022, 25, 2; 325-334
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Dispersal and immunohistochemical characteristics of neurons in the stem of the porcine vagus nerve
    Autorzy:
    Sienkiewicz, W.
    Klimczuk, M.
    Majewska, M.
    Kaleczyc, J.
    Powiązania:
    https://bibliotekanauki.pl/articles/16647386.pdf
    Data publikacji:
    2022
    Wydawca:
    Polska Akademia Nauk. Czasopisma i Monografie PAN
    Tematy:
    vagus nerve
    intravagal neurons
    neurotransmitter markers
    immunohistochemistry
    pig
    Opis:
    The present study investigated the distribution and chemical properties of nerve cell bodies within the trunk of the vagus nerve in juvenile female pigs (n=4) using double-labelling immunofluorescence. The neurons appeared mostly as single cells or formed streaks of cells or small ganglia. Many of the perikarya were cholinergic (VAChT-positive; VAChT+) or adrenergic (DβH+) in nature and no SP+ or CGRP+ neurons were encountered. There were no distinct left-right differences regarding the number and chemical coding of the neuronal somata, however, these characteristics significantly varied between particular nerve segments investigated. The vagosympathetic trunks, and thoracic and abdominal segments of the vagus nerve contained on average (the numerical values represent the means for both the left and right corresponding nerve segments) 142, 236, and 111 PGP 9.5-positive neurons, respectively. Proportions of cholinergic and adrenergic neurons were as follows: 0% and 100%, 54.2% and 33.2%, and 52.8% and 35.4%, respectively. Relatively many neurons in the thoracic and abdominal segments stained also for NOS (39.2% and 39.9%, respectively). It remains to be determined whether the porcine intravagal neurons represent a developmental relic, or whether they have any specific functional significance.
    Źródło:
    Polish Journal of Veterinary Sciences; 2022, 25, 4; 617-619
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Immunohistochemical properties of motoneurons supplying the trapezius muscle in the rat
    Autorzy:
    Dudek, A.
    Sienkiewicz, W.
    Marczak, M.
    Kaleczyc, J.
    Powiązania:
    https://bibliotekanauki.pl/articles/30477.pdf
    Data publikacji:
    2011
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    immunohistochemical property
    motoneuron
    trapezius muscle
    rat
    tracing
    immunohistochemistry
    Opis:
    Combined retrograde tracing (using fluorescent tracer Fast blue) and double-labelling immunofluorescence were used to study the distribution and immunohistochemical characteristics of neurons projecting to the trapezius muscle in mature male rats (n=9). As revealed by retrograde tracing, Fast blue-positive (FB+) neurons were located within the ambiguous nucleus and accessory nucleus of the grey matter of the spinal cord. Immunohistochemistry revealed that nearly all the neurons were cholinergic in nature [choline acetyltransferase (ChAT)-positive]. Retrogradely labelled neurons displayed also immunoreactivities to calcitonin gene-related peptide (CGRP; approximately 60% of FB+ neurons), nitric oxide synthase (NOS; 50%), substance P (SP; 35%), Leu5-Enkephalin (LEnk; 10%) and vasoactive intestinal polypeptide (VIP; 5%). The analysis of double-stained tissue sections revealed that all CGRP-, VIP- and LEnk-immunoreactive FB+ perikarya were simultaneously ChAT-positive. The vast majority of the neurons expressing SP- or NOS-immunoreactivity were also cholinergic in nature; however, solitary somata were ChAT-negative. FB+ perikarya were surrounded by numerous varicose nerve fibres (often forming basket-like structures) immunoreactive to LEnk or SP. They were also associated with some CGRP-, NOS- and neuropeptide Y-positive nerve terminals.
    Źródło:
    Polish Journal of Veterinary Sciences; 2011, 14, 2
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Określenie wartości diagnostycznej wybranych badań immunohistochemicznych u chorych na mięsaki macicy w aspekcie ich przydatności terapeutycznej i prognostycznej
    Determination of diagnostic value of selected immunohistochemical tests in patients with uterine corpus sarcoma and their therapeutic and prognostic usefulness
    Autorzy:
    Jońska-Gmyrek, Joanna
    Nasierowska-Guttmejer, Anna
    Bidziński, Mariusz
    Dańska-Bidzińska, Anna
    Powiązania:
    https://bibliotekanauki.pl/articles/908070.pdf
    Data publikacji:
    2007
    Wydawca:
    Medical Communications
    Tematy:
    corpus uteri sarcoma
    immunohistochemistry
    markers
    treatment
    diagnosis
    immunohistochemia
    Opis:
    Uterine sarcomas represent a rare and heterogenous group of tumours, sometimes demonstrating multidirectional differentiation. Histological type of the sarcoma influences the choice of therapeutic modality. Aim of paper: Determination of diagnostic value of selected immunohistochemical studies (MIB1, caldesmon, desmin, SMA, SrMa, CKAE1/3) in the definition of histological type of tumour. Assessment of expression of the protein c-kit (CD117) MIB1 and inhibin and their prognostic role. Material and method: Study material consisted of a group of patients with uterine sarcoma, treated at the Center of Oncology in Warsaw, Poland, since 1999 thru 2006, from whom surgical specimens and paraffin blocks were obtained. Of 120 eligible patients, complete clinical data and specimens suitable for foreseen studies were available in 66 cases and this group became the subject of further analyses. Immunohistochemical (IHC) tests were performed using a panel of antibodies (SMA, SrMa, desmin, h-caldesmon, CD10, CKAE, MIB1, inhibin and CD117). Diagnostic value of IHC studies was assessed by descriptive statistical tests. Original histopathological findings were confronted with the results of validated IHC tests. Survival was assessed based on Kaplan-Meier curves and follow-up time – by an inverse Kaplan-Meier curve. Prognostic factors were analysed by the Cox method. Threshold of statistical significance has been set at p=0.05. Results: Original microscopic findings have had to be modified as to histological type of sarcoma in about 1/3 of the cases. Histological diagnosis was an independent prognostic factor, influencing both overall survival rate and time to tumour recurrence. Prognostic factors influencing recurrence-free survival were: invasion of perivascular lymph space (lymph space invasion, LSI), proliferative index (MB1) and severity of tumour cell necrosis. Conclusions: Application of a panel of suitable IHC tests contributes to a more precise determination of tumour type, assisting in an optimal choice of adjuvant treatment modality and thus in improvement of the patients’ prognosis.
    Mięsaki macicy stanowią rzadko występującą, heterogenną grupę nowotworów, wykazującą niekiedy wielokierunkowe różnicowanie. Typ histopatologiczny mięsaka ma wpływ na wybór metody leczenia. Cel: Określenie przydatności diagnostycznej wybranych badań immunohistochemicznych (MIB1, kaldesmon, desmi-na, SMA, SrMa, CKAE1/3) w ocenie typu histopatologicznego. Ocena ekspresji białka c-kit (CD117), MIB1, oraz inhibiny w aspekcie przydatności prognostycznej. Materiał i metoda: Materiał badawczy stanowiła grupa chorych na mięsaki macicy leczonych w Centrum Onkologii - Instytucie im. Marii Skłodowskiej--Curie w Warszawie w latach 1999-2006, od których uzyskano preparaty i bloczki parafinowe. Spośród 120 chorych pełne dane kliniczne i preparaty nadające się do zastosowania badań uzyskano od 66 chorych, która to grupa stała się przedmiotem analizy. Wykonano badania immunohistochemiczne z użyciem panelu przeciwciał: SMA, SrMa, desmina, h-kaldesmon, CD10, CKAE, MIB1, inhibina oraz CD117. Do oceny wartości diagnostycznej badań immunohistochemicznych (IHC) zastosowano statystykę opisową. Zestawiono wynik pierwotnego badania histopatologicznego z wynikiem zweryfikowanym badaniami IHC. Do oceny przeżycia zastosowano krzywą Kaplana-Meiera, czas obserwacji mierzono odwrotną metodą Kaplana-Me-iera. Do analizy czynników predykcyjnych zastosowano metodę Coksa. Istotność statystyczną określono na poziomie p=0,05. Wyniki: W stosunku do rozpoznania pierwotnego typ histopatologiczny mięsaków został zmieniony w około 1/3 przypadków. Rozpoznanie histopatologiczne okazało się niezależnym czynnikiem rokowniczym mającym wpływ zarówno na przeżycie ogólne, jak i czas do nawrotu choroby. Czynnikami rokowniczymi w aspekcie przeżycia bez nawrotu choroby okazały się: zajęcie naczyniowej przestrzeni chłonnej (LSI), indeks proliferacyjny (MIB1), oraz stopień martwicy w komórkach guza. Wnioski: Zastosowanie panelu odpowiednich badań immunohistochemicznych ma wpływ na precyzyjne określenie charakteru guza, co niesie za sobą wybór odpowiedniej metody leczenia uzupełniającego, a tym samym, być może poprawę rokowania.
    Źródło:
    Ginekologia Onkologiczna; 2007, 5, 2; 75-86
    1731-5379
    Pojawia się w:
    Ginekologia Onkologiczna
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Enteric neuronal development in canine small intestine – an immunohistochemical study
    Autorzy:
    Serzysko, T.
    Skwarek, A.
    Chudziak, E.
    Malina, M.
    Kaleczyc, J.
    Sienkiewicz, W.
    Powiązania:
    https://bibliotekanauki.pl/articles/2087175.pdf
    Data publikacji:
    2021
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    dog
    fetus
    small intestine
    enteric nervous system
    development
    neurons
    immunohistochemistry
    Źródło:
    Polish Journal of Veterinary Sciences; 2021, 24, 2; 293-301
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Distribution and chemical coding of urinary bladder apex-projecting neurons in aorticorenal and testicular ganglia of the male pig
    Autorzy:
    Pidsudko, Z.
    Listowska, Ż.
    Franke-Radowiecka, A.
    Klimczuk, M.
    Załęcki, M.
    Kaleczyc, J.
    Powiązania:
    https://bibliotekanauki.pl/articles/2087510.pdf
    Data publikacji:
    2019
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    prevertebral ganglia
    urinary bladder apex
    immunohistochemistry
    retrogradetracing
    neuropeptides
    male pig
    Źródło:
    Polish Journal of Veterinary Sciences; 2019, 2; 427-430
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Nesfatin-1 prevents negative changes in bone in conditions of developing osteopenia
    Autorzy:
    Puzio, I.
    Tymicki, G.
    Pawłowska, M.
    Bieńko, M.
    Radzki, R.P.
    Powiązania:
    https://bibliotekanauki.pl/articles/2085429.pdf
    Data publikacji:
    2020
    Wydawca:
    Instytut Medycyny Wsi
    Tematy:
    nesfatin-1
    osteopenia
    osteoporosis
    ovariectomy
    rat
    DXA
    pQCT
    immunohistochemistry
    Opis:
    Objective. The aim of the study was to determine the effect of nesfatin-1 on bone properties in female rats in the conditions of developing osteopenia induced by ovariectomy (OVX). Materials and method. The experiment was performed on 21 female Wistar rats assigned to 3 groups receiving intraperitoneally physiological saline (SHO, OVX-PhS) and nesfatin-1 in dose 2 μg/kg BW of (OVX-NES) once a day for 8 wks. At the end of the experiment, the rats were scanned using the DXA method to determine the body composition, tBMC, and tBMD. The isolated femora and tibia were tested with the DXA method for BMD and BMC, and with the pQCT method for separate analysis of the cortical and trabecular bone tissue. The bone strength parameters were also determined. The immunohistochemical method was used for determination of nesfatin-1 localization in growth cartilage. Bone metabolism markers (osteocalcin, bALP, and NTx) were identified using an ELISA kit. Results. OVX exerts a negative effect on bone tissue. The nesfatin-1 administration influenced positively the DXA parameters of tibia. TvBMD and TbvBMD measured by pQCT in metaphysis of bones were significantly higher in the OVX-NES group than in OVX-PhS. No differences were found in the values of bone strength parameters between SHO and OVX-NES females. Extra- and intracellular immunohistochemical reaction for nesfatin-1 was observed in all zones of growth cartilage, with the strongest reaction detected in the calcifying zone. Nesfatin-1 administration caused a significant increase in the osteocalcin and bALP concentration in relation to the OVX-PhS animals. Conclusion. The results of the experiment indicate that nesfatin-1 exerts a protective effect on bone tissue properties and can be used in the prevention of osteoporosis.
    Źródło:
    Annals of Agricultural and Environmental Medicine; 2020, 27, 1; 66-75
    1232-1966
    Pojawia się w:
    Annals of Agricultural and Environmental Medicine
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Differential relationship between two hypoxia markers: HIF-1α and GLUT1 and classic prognostic factors in invasive breast carcinoma
    Zróżnicowana zależność pomiędzy dwoma markerami hipoksji: HIF-1α i GLUT1 a klasycznymi czynnikami prognostycznymi w inwazyjnym raku piersi
    Autorzy:
    Żyromska, Agnieszka
    Andrusewicz, Hanna
    Łysik, Joanna
    Jóźwicki, Wojciech
    Wiśniewski, Tomasz
    Powiązania:
    https://bibliotekanauki.pl/articles/1029826.pdf
    Data publikacji:
    2016
    Wydawca:
    Medical Communications
    Tematy:
    GLUT1
    HIF-1α
    hypoxia
    immunohistochemistry
    prognostic factors
    Opis:
    Background: Tumor hypoxia is an adverse prognostic factor which promotes cancer aggressiveness and limits its radio- and chemosensitivity. The aim of our study was to explore the relationship between endogenous hypoxia markers and classic prognostic factors, including clinical stage and the expression of ER, PR, and HER2 in primary untreated breast carcinoma. Methods: A retrospective immunohistochemical analysis of archived tissue blocks collected from 153 women, who underwent total mastectomy and lymph node dissection, included the expression of two hypoxia-related proteins: HIF-1α and GLUT1. Results: GLUT1 labelling index (LI) showed a positive correlation with T stage (R = 0.18, p = 0.026) and HER2 status (R = 0.25, p = 0.002), and a negative correlation with the expression of ER (R = −0.19, p = 0.017) and PR (R = −0.17, p = 0.032). HIF-1α LI showed a positive correlation with ER expression (R = 0.16, p = 0.045). In the multivariate regression analysis, a different relationship between classic prognostic factors and the two tested hypoxia proteins was proven. Higher GLUT1 expression correlated with ER and PR negativity (p = 0.02 and p = 0.01, respectively) as well as with higher expression of HER2 (p = 0.04). HIF-1α showed no association with PR and HER2, but a positive correlation with ER (p = 0.02). Neither of the hypoxia proteins was associated with a tumor grade. Only one clinical feature, T stage, correlated with both of the hypoxia markers: positively with GLUT1 (p = 0.049) and negatively with HIF-1α (p = 0.01) expression. Conclusions: In breast cancer, GLUT1 expression may be considered an additional prognostic factor which correlates with an adverse status of HER2 and hormonal receptors, and indicates a more hypoxic, radio- and chemotherapy refractory profile of carcinoma.
    Tło: Hipoksja w  guzie nowotworowym stanowi niekorzystny czynnik prognostyczny, ogranicza jego promienioi chemiowrażliwość oraz promuje bardziej agresywny przebieg choroby. Przewidywanie rokowania i odpowiedzi na leczenie wymaga wiedzy o związku hipoksji z uznanymi czynnikami prognostycznymi. Celem badania było określenie zależności pomiędzy endogennymi markerami hipoksji w  pierwotnym przewodowym raku piersi a  klasycznymi czynnikami prognostycznymi, takimi jak stopień zaawansowania klinicznego oraz ekspresja receptorów ER, PR i HER2. Metody: Retrospektywna analiza immunohistochemiczna archiwizowanych bloczków tkanek pobranych od 153 kobiet, poddanych mastektomii i limfadenektomii pachowej, objęła ekspresję dwóch związanych z hipoksją białek: HIF-1α i GLUT1. Wyniki: Indeks wiązania GLUT1 (GLUT1 LI) wykazał korelację dodatnią z wielkością guza (R = 0,18, p = 0,026) i ekspresją HER2 (R = 0,25, p = 0,002) oraz ujemną z ekspresją ER (R = −0,19, p = 0,017) i PR (R = −0,17, p = 0,032). HIF-1α LI korelował wyłącznie z ekspresją ER (R = 0,16, p = 0,045). W analizie wieloczynnikowej wykazano zróżnicowaną zależność pomiędzy klasycznymi czynnikami prognostycznymi i testowanymi markerami hipoksji. GLUT1 LI korelował negatywnie z ekspresją ER i PR (odpowiednio p = 0,02 i p = 0,01) oraz pozytywnie z ekspresją HER2 (p = 0,04). Nie udowodniono korelacji pomiędzy HIF-1α LI a ekspresją PR czy HER2, natomiast wykazano jego dodatnią zależność z ekspresją ER (p = 0,02). Żaden marker hipoksji nie korelował ze stopniem zróżnicowania histologicznego nowotworu. Tylko jeden kliniczny czynnik – wielkość guza (T) – korelował z ekspresją badanych białek: dodatnio z GLUT1 (p = 0,049), a ujemnie z HIF-1α (p = 0,01). Wnioski: Ekspresja GLUT1 w raku piersi może stanowić dodatkowy czynnik prognostyczny, korelujący z niekorzystnym statusem receptora HER2 i receptorów hormonalnych oraz wskazywać na bardziej hipoksyczny, oporny na radioi chemioterapię, profil raka.
    Źródło:
    Current Gynecologic Oncology; 2016, 14, 4; 197-203
    2451-0750
    Pojawia się w:
    Current Gynecologic Oncology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    The distribution and chemical coding of urinary bladder trigone-projecting neurons in testicular and aorticorenal ganglia in male pigs
    Autorzy:
    Pidsudko, Z.
    Godlewski, J.
    Wąsowicz, K.
    Powiązania:
    https://bibliotekanauki.pl/articles/16539341.pdf
    Data publikacji:
    2022
    Wydawca:
    Polska Akademia Nauk. Czasopisma i Monografie PAN
    Tematy:
    prevertebral ganglia
    urinary bladder trigone
    immunohistochemistry
    retrograde tracing
    neuropeptides
    male pig
    Opis:
    Combined retrograde tracing and double-labelling immunofluorescence were used to investigate the distribution and chemical coding of neurons in testicular (TG) and aorticoerenal (ARG) ganglia supplying the urinary bladder trigone (UBT) in juvenile male pigs (n=4, 12 kg. of body weight). Retrograde fluorescent tracer Fast Blue (FB) was injected into the wall of the bladder trigone under pentobarbital anesthesia. After three weeks all the pigs were deeply anesthetized and transcardially perfused with 4% buffered paraformaldehyde. TG and ARG, were collected and processed for double-labelling immunofluorescence. The expression of tyrosine hydroxylase (TH) or dopamine beta-hydroxylase (DBH), neuropeptide Y (NPY), somatostatin (SOM), galanin (GAL), nitric oxide synthase (NOS) and vesicular acetylcholine transporter (VAChT) were investigated. The cryostat sections were examined with a Zeiss LSM 710 confocal microscope equipped with adequate filter blocks. The TG and ARG were found to contain many FB-positive neurons projecting to the UBT (UBT-PN). The UBT-PN were distributed in both TG and ARG. The majority of them were found in the right ganglia, mostly in TG. Immunohistochemistry disclosed that the vast majority of UBT-PN were noradrenergic (TH- and/or DBH-positive). Many noradrenergic neurons contained also immunoreactivity to NPY, SOM or GAL. Most of the UBT-PN were supplied with VAChT-, or NOS- IR (immunoreactive) varicose nerve fibres. This study has revealed a relatively large population of differently coded prevertebral neurons projecting to the porcine urinary bladder. As judged from their neurochemical organization these nerve cells constitute an important element of the complex neuro-endocrine system involved in the regulation of the porcine urogenital organ function.
    Źródło:
    Polish Journal of Veterinary Sciences; 2022, 25, 3; 483-487
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Iridociliary epithelial tumor in a cat
    Autorzy:
    Ito, S.
    Narasaki, Y.
    Nakano, Y.
    Minami, T.
    Kanemaki, N.
    Powiązania:
    https://bibliotekanauki.pl/articles/9938.pdf
    Data publikacji:
    2012
    Wydawca:
    Okulistyka Weterynaryjna
    Tematy:
    cat
    animal disease
    ocular disease
    iridociliary epithelial tumour immunohistochemistry
    tumour
    Opis:
    A 15-year-old female spayed domestic short-hair cat was referred for a nonpigmented mass within the pupil of the left eye. In the ophthalmic examination, the non-pigmented mass appeared behind the iris. Visual function was normal, and there were no significant clinical findings except the ciliary body mass. Seven days after presentation, the mass was removed with sclerotomy and iridocyclectomy. The mass was determined to be an iridociliary epithelial tumor upon histopathological and immunohistochemical analyses. Thirty-eight days after tumor removal, visual function was maintained, and the papillary light reflex remained normal.
    A 15-year-old female spayed domestic short-hair cat was referred for a non-pigmented mass within the pupil of the left eye. In the ophthalmic examination, the non-pigmented mass appeared behind the iris. Visual function was normal, and there were no significant clinical findings except the ciliary body mass. Seven days after presentation, the mass was removed with sclerotomy and iridocyclectomy. The mass was determined to be an iridociliary epithelial tumor upon histopathological and immunohistochemical analyses. Thirty-eight days after tumor removal, visual function was maintained, and the papillary light reflex remained normal.
    Źródło:
    Okulistyka Weterynaryjna. e-kwartalnik dla lekarzy i studentów weterynarii; 2012, 3
    2082-9256
    Pojawia się w:
    Okulistyka Weterynaryjna. e-kwartalnik dla lekarzy i studentów weterynarii
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Immediate and Delayed Surgical Repair of Duodenal Defects in Rats with Small Intestinal Submucosa Patch
    Autorzy:
    Miliaras, Dimosthenis
    Miliaras, Spyridon
    Meditskou, Soultana
    Papamitsou, Theodora
    Powiązania:
    https://bibliotekanauki.pl/articles/1395733.pdf
    Data publikacji:
    2014-03-01
    Wydawca:
    Index Copernicus International
    Tematy:
    duodenal defect
    healing
    immunohistochemistry
    regeneration
    small intestinal submucosa
    TGF-β
    Opis:
    Duodenal injuries, though rare, carry high rates of morbidity and mortality. The aim of the study was to evaluate the healing of the duodenal wall with the use of a Small Intestinal Submucosa (SIS) patch. Material and methods. We studied 40 Whistar-Albino rats divided into two groups. In group A, we created a small defect in the duodenal wall, which was immediately covered with a SIS patch. In group B, the SIS patch was sutured over the defect after 6-8 hours, in order to induce peritonitis. The animals of both groups were sacrificed after 2, 6, 12 and 16 weeks respectively. In addition, we studied the immunohistochemical expression of TGF-β, which is a major constituent of SIS, and plays a central role in the healing process. Results. Histology showed progressive development of the layers of the duodenal wall over the patch as early as the 2nd week in some of the animals of group A. Mucosa developed later on in the animals of group B, presumably due to the more intense inflammation elicited by peritonitis. Expression of TGF-β was initially more pronounced in the epithelial cells of the regenerating mucosa of animals of group A, but it was maintained in higher levels in the animals of group B, which showed delayed mucosa degeneration. Conclusions. SIS appears to be both efficient and safe for the management of duodenal trauma. TGF-β seems to play an important role in the healing process, inducing regeneration of the stroma, and controling epithelial growth.
    Źródło:
    Polish Journal of Surgery; 2014, 86, 3; 116-121
    0032-373X
    2299-2847
    Pojawia się w:
    Polish Journal of Surgery
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Immunohistochemical study on the expression of biologically active substances in the endocrine pancreas of the European bison
    Autorzy:
    Mozel, S.
    Szymańczyk, S.
    Krzysiak, M.
    Puzio, I.
    Zacharko-Siembida, A.
    Arciszewski, M.B.
    Powiązania:
    https://bibliotekanauki.pl/articles/2087293.pdf
    Data publikacji:
    2020
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    glucagon-like peptide-1
    glucagon
    insulin
    somatostatin
    immunohistochemistry
    pancreas
    European bison
    Źródło:
    Polish Journal of Veterinary Sciences; 2020, 23, 3; 333-340
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Przydatność mikroskopii elektronowej w diagnostyce gruczolaków przysadki
    Usefulness of electron microscopy methods in the diagnostics of pituitary adenomas
    Autorzy:
    Maksymowicz, Maria
    Olszewski, Włodzimierz
    Powiązania:
    https://bibliotekanauki.pl/articles/1032924.pdf
    Data publikacji:
    2010
    Wydawca:
    Łódzkie Towarzystwo Naukowe
    Tematy:
    przysadka
    gruczolaki
    ultrastruktura
    immunohistochemia
    metody diagnostyczne
    pituitary
    adenomas
    ultrastructure
    immunohistochemistry
    diagnostic methods
    Opis:
    Pituitary adenomas represent the most common neoplasms of sella turcica. They are clinically differentiated mainly on the basis of their size, radiological features and hormonal secretion. The patients present symptoms of acromegaly, hyperprolactinemia, Cushing's disease, Nelson syndrome, TSH hypersecretion or clinically nonfunctioning adenomas. The lack of clinicopathological correlations in this tumors limit the importance of routine histological diagnosis. In the current WHO (2004) classification of pituitary adenomas immuno-histochemical evaluation is the “gold diagnostic standard”. However, immunohistochemical profiles may overlap, but ultrastructural features of these tumors are essential to reach a correct diagnosis. EM methods are capable of providing specific diagnosis in the majority of the rare adenoma subtypes with specific biological behavior, invasiveness, aggressive growth potential and recurrences. Our material consists of 2010 consecutive cases of surgically resected pituitary and sellar region tumors diagnosed in our department. Among them 1783 cases of pituitary adenomas were diagnosed. Ultrastructural evaluation was useful in: GH secreting adenomas - sparsely vs. densely granulated; bi-hormonal GH and PRL secreting adenomas; clinically nonfunctioning immunopositive (silent) adenomas; clinically nonfunctioning adenomas - nononcocytic vs. oncocytic; unusual plurihormonal adenomas - monomorphous vs. mixed cell. Immunoelectron microscopy was valuable in bi-hormonal adenomas: GH and PRL immunopositive. Conclusions: Currently, there are no accepted means of predicting adenoma’s invasiveness and long-term aggressiveness, but morphologic separation of some subtypes of pituitary adenomas by EM provided useful knowledge for classification and management. Our own data confirmed the usefulness of ultrastructural methods in diagnostic and prognostic evaluation of pituitary adenomas.
    Gruczolaki przysadki są najczęstszymi nowotworami występującymi w okolicy siodła tureckiego. Klinicznie są one klasyfikowane głównie na podstawie rozmiarów, cech radiologicznych i czynności hormonalnej. Pacjenci prezentują objawy akromegalii, hiperprolaktynemii, choroby Cushinga, zespołu Nelsona, hipertyreozy lub objawy guzów klinicznie niemych. Brak w tych guzach korelacji między obrazem klinicznym i patomorfologicznym, co ogranicza znaczenie rutynowej diagnostyki histologicznej. W aktualnej klasyfikacji Światowej Organizacji Zdrowia (2004) „złoty standard diagnostyczny” stanowi immunohistochemia. JednakŜe profile immunohistochemiczne mogą się pokrywać, zaś cechy ultrastrukturalne tych guzów mogą stanowić podstawę prawidłowego rozpoznania. Metody mikroskopowo-elektronowe umoŜliwiają rozpoznanie większości rzadkich podtypów gruczolaków o swoistej biologii i inwazyjności, agresywnym potencjale wzrostowym oraz skłonności do nawrotów. Materiał własny obejmuje 2010 nieselekcjonowanych przypadków guzów przysadki i okolicy siodła tureckiego leczonych chirurgicznie w latach 1998-2010, wśród których rozpoznano 1783 gruczolaki. Ocena ultrastrukturalna była przydatna w: gruczolakach wydzielających GH – skąpoziarniste vs. bogatoziarniste, dwu-hormonalnych gruczolakach wydzielających GH i PRL, gruczolakach klinicznie nieczynnych hormonalnie, ale immunododatnich (niemych), klinicznie nieczynnych – onkocytarne vs. nieonkocytarne; rzadkich gruczolakach wielohormonalnych – monomorficzne vs. mieszane. Metoda immunomikroskopowo- elektronowa była przydatna w dwu-hormonalnych gruczolakach wydzielających GH i PRL. Wnioski: Obecnie brak ogólnie akceptowanych metod oceny przewidywania inwazyjności lub agresywności, ale rozpoznanie niektórych podtypów gruczolaków metodą mikroskopii elektronowej dostarcza podstawowych informacji umoŜliwiających ich sklasyfikowanie i prawidłowe postępowanie pooperacyjne. Nasze własne obserwacje oraz dane z piśmiennictwa potwierdzają przydatność metod ultrastrukturalnych w diagnostycznej i prognostycznej ocenie tych guzów.
    Źródło:
    Folia Medica Lodziensia; 2010, 37, 1; 151-173
    0071-6731
    Pojawia się w:
    Folia Medica Lodziensia
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Distribution and amount of cathepsin B in gentamicin-induced acute kidney injury in rats
    Autorzy:
    Svara, T
    Pogacnik, M.
    Juntes, P.
    Powiązania:
    https://bibliotekanauki.pl/articles/30766.pdf
    Data publikacji:
    2010
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    rat
    kidney
    cathepsin B
    gentamicin
    distribution
    immunohistochemistry
    vacuolar degeneration
    histopathology
    statistical method
    Opis:
    The aim of our study was to investigate how the distribution and amount of cathepsin B change during acute kidney injury. The research was done on a rat model of acute kidney injury that was induced by nephrotoxic antibiotic gentamicin. Gentamicin was injected at a dose of 40 mg/kg body weight (the first treated group) and 80 mg/kg body weight (the second treated group) for 14 days. Control groups received injections of physiological saline only. One day after the last injection, animals were euthanized, dissected and kidney samples were taken and fixed in 10% buffered formalin. Tissue sections were stained with haematoxylin and eosin, periodic Acid Schiff (PAS) and Oil-red-O. Immunohistochemistry was used for the demonstration of cathepsin B. Vacuolar degeneration of the proximal convoluted tubules was the most prominent pathologic lesion found in the first treated group, while necrosis prevailed in the second treated group in the same localisation. In both treated groups significantly weaker immunohistochemical reaction for cathepsin B was noticed in the proximal convoluted tubules in comparison to the control groups (P < 0.05). The decrease of positive reaction was the largest in the proximal convoluted tubules of the outer renal cortex. Stronger positive reaction for cathepsin B, although not statistically significant, was found in the proximal straight tubules (P > 0.05), as well. However, more numerous cathepsin B-positive large granules appeared in the proximal straight tubules of the second treated group then in the second control group (P < 0.05). We can conclude that the amount of cathepsin B in the affected proximal convoluted tubules significantly decreases along the increased severity of the histopathological lesions of the proximal convoluted tubuls, the amount of enzyme in the well preserved proximal straight tubules increases and more cathepsin B-positive large granules appear in the cytoplasm.
    Źródło:
    Polish Journal of Veterinary Sciences; 2010, 13, 1; 75-82
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Immunohistochemical study of expression of immunoglobulins in canine B-cell lymphomas
    Autorzy:
    Sokolowska, J.
    Micun, J.
    Zabielska, K.
    Malicka, E.
    Lechowski, R.
    Powiązania:
    https://bibliotekanauki.pl/articles/31685.pdf
    Data publikacji:
    2010
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    immunohistochemistry
    expression
    immunoglobulin
    dog
    B cell
    lymphoma
    tumour
    neoplastic cell
    immunoglobulin light chain
    cancer
    Opis:
    Nineteen canine lymphomas were included in this study. Tumors were classified according to the updated Kiel classification adapted for canine lymphomas by Fournel-Fleury et al. Immunoglobulin light chains (κ and λ) and IgM and IgG expression were determined by immunohistochemical method. In all examined cases neoplastic cells were positive for one of the immunoglobulin light chains. Expression of λ light chains and κ light chains was observed in 18/19 and 1/19 tumors, respectively. In the majority of neoplastic cells in each examined specimen this reaction had a membranous pattern (sκ/sλ). In all examined cases the presence of immunoglobulin light chains was also observed in the cytoplasm of some neoplastic cells (cκ/cλ). These cells were usally rare and never constituted a dominant population. The expression of immunoglobulin was found in 13/19 cases. Most lymphomas were sIgM positive (11/13 cases). In one case expression of IgG was found, and in another lymphoma two populations of neoplastic cells with different expression of examined immunoglobulins (cells with IgM+ and IgG+ phenotypes) were observed. The reaction also had a membranous pattern. The cells containing cytoplasmic immunoglobulins were rare, and in most cases were of the same type as the surface immunoglobulins. Our study has confirmed that canine lymphomas are a monoclonal proliferation of B-cells usually expressing immunoglobulin λ light chains and that the vast majority of tumors deriving from B-cells express IgM. Our study also indicates a possibility of occurence of biclonal lymphomas in canine species.
    Źródło:
    Polish Journal of Veterinary Sciences; 2010, 13, 4
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Znaczenie prognostyczne ekspresji HER-2/neu u chorych na wczesne postacie inwazyjnego raka szyjki macicy
    Prognostic significance of HER-2/neu expression in patients at early clinical stages of invasive cervical cancer
    Autorzy:
    Panek, Grzegorz
    Ligaj, Marcin
    Powiązania:
    https://bibliotekanauki.pl/articles/908432.pdf
    Data publikacji:
    2007
    Wydawca:
    Medical Communications
    Tematy:
    HER-2/neu expression
    cervical cancer
    immunohistochemistry
    prognostic factors
    survival rate and mortality rate
    Opis:
    HER-2/neu overexpression is considered an important prognostic factor, which may predict an aggressive clinical course of several tumours, including cervical cancer. Material and method: HER-2/neu expression was assessed by immunohistochemical technique in 298 patients with stage IB and IIA cervical cancer, of either planoepithelial or adenomatous type. HER-2/neu expression was compared in groups of patients selected based on generally accepted prognostic factors, e.g. clinical stage, histological type, presence of metastases to regional lymph nodes, invasion of vascular space and degree of radiation-induced destruction of tumor. Using the Cox model, a multivariate analysis of effect of selected prognostic factors was performed, both in relation to overall survival and to symptom-free survival. Results: Positive reaction to HER-2/neu was obtained in 135 cases (45.3%); the reaction was pronounced (3+) in 23 cases (7.8%) and moderate (2+) in 41 cases (13.7%). No positive reaction was obtained in 163 cases (54.7%). Analysis of survival yielded the following results: probability of 5-year overall survival for the entire patient population was 90.9% and that of 10 years – 85.2%. Overall survival curve for the entire study population is presented. Recurrence-free 5- and 10-year survival rates were 83.3% and 82.3%, respectively. Symptom-free 5- and 10-year survival rates were significantly influenced by: lymph node involvement and their number, invasion of vascular space, histological type and HER-2/neu expression. Cox analysis demonstrated a significant correlation between risk of recurrence or death and the following factors: lymph node metastases and their number, invasion of vascular space, histological type of tumour and HER-2/neu expression. The risk of recurrence or death with a single nodal metastasis and several nodal metastases is 2.4- and 5.1-fold greater than when no nodal metastases are present. The fact of vascular space involvement results in a 3-fold increase of risk of recurrence or death. In the case of planoepithelial cancer, the risk of recurrence or death is 0.4-fold that for adenomatous cancer. Finding of a strong HER-2/neu expression (2+, 3+) increases 2.7-fold the risk of therapeutic failure. Conclusions: No statistically significant differences were found in the HER-2/neu expression in groups of patients defined based on clinical-pathological parameters (i.e. clinical stage, histological type, regional lymph node metastases, radiation-induced cervix sterilization). The following prognostic factors had a significant impact on symptom-free survival: histological type, presence and numbers of lymph node metastases, invasion of vascular space, as well as TP53, BCL2 and HER-2/neu expression. Among all factors analysed, most significant prognostic impact, both in terms of overall survival and symptom-free survival, was associated with involvement of pelvic lymph nodes.
    Nadekspresja HER-2/neu uważana jest za istotny czynnik prognostyczny mogący wskazywać na agresywny przebieg kliniczny wielu nowotworów, w tym RSM. Materiał i metody: Posługując się metodą immunohistoche-miczną, przeprowadzono ocenę ekspresji HER-2/neu u 298 chorych leczonych z powodu RSM w stopniach IB i IIA zarówno w grupie chorych na raka płaskonabłonkowego, jak i gruczołowego. Dokonano porównania ekspresji HER-2/neu w grupach chorych wyróżnionych na podstawie uznanych czynników prognostycznych, takich jak stopień klinicznego zaawansowania, typ histologiczny, obecność przerzutów do regionalnych węzłów chłonnych, zajęcie przestrzeni naczyniowej i stopień uszkodzenia popromiennego nowotworu. Posługując się modelem Coksa, przeprowadzono wielowariantową analizę wpływu ocenianych czynników prognostycznych zarówno na przeżycia całkowite, jak i bezobjawowe. Wyniki: Pozytywną reakcję na HER-2/neu stwierdzono w 135 przypadkach (45,3%): w 23 przypadkach była to reakcja o dużym nasileniu - 3+ (7,8%), a w 41 przypadkach o nasileniu średniego stopnia - 2+ (13,7%). W 163 przypadkach (54,7%) nie stwierdzono pozytywnej reakcji. Wyniki analizy przeżyć są następujące: prawdopodobieństwo całkowitego przeżycia 5 lat dla całej grupy chorych wyniosło 90,9%, a 10 lat - 85,2%. Przedstawiono krzywą przeżycia całkowitego analizowanej grupy chorych. Prawdopodobieństwo przeżycia bez nawrotu choroby 5 i 10 lat wyniosło odpowiednio 83,3% i 82,3%. Istotny wpływ na 5- i 10-letnie przeżycia bezobjawowe miały: przerzuty do węzłów chłonnych i ich liczba, zajęcie przestrzeni naczyniowej, typ histologiczny i ekspresja HER-2/neu. W wyniku przeprowadzonej analizy Coksa wykazano istotny wpływ prognostyczny na ryzyko nawrotu lub zgonu takich czynników, jak: przerzuty do węzłów chłonnych i ilość zajętych węzłów, zajęcie przestrzeni naczyniowej, typ histologiczny i ekspresja HER-2/neu. Ryzyko nawrotu lub zgonu dla jednego zajętego węzła oraz dla większej liczby zajętych węzłów chłonnych jest 2,4 oraz 5,1 razy wyższe od odpowiedniego ryzyka dla węzłów niezajętych. Fakt zajęcia przestrzeni naczyniowej zwiększa niemal 3-krotnie ryzyko nawrotu lub zgonu. Dla raka płaskonabłon-kowego ryzyko nawrotu lub zgonu stanowi 0,4 ryzyka niepowodzenia dla raka gruczołowego. Stwierdzenie silnej ekspresji HER-2/neu (2+, 3+) zwiększa 2,7 razy ryzyko niepowodzenia leczenia. Wnioski: Nie stwierdzono istotnych statystycznie różnic w ekspresji HER-2/neu w grupach chorych wyróżnionych na podstawie parametrów kliniczno-patologicznych (tj.: stopnia klinicznego zaawansowania, typu histologicznego, przerzutów do regionalnych węzłów chłonnych i popromiennej sterylizacji szyjki macicy). Istotny wpływ statystyczny na przeżycia bezobjawowe miały następujące czynniki prognostyczne: typ histologiczny, przerzuty do węzłów chłonnych i ich liczba, inwazja przestrzeni naczyniowej oraz ekspresja TP53, BCL2 i HER-2/neu. Spośród wszystkich analizowanych czynników największy wpływ prognostyczny zarówno na przeżycia całkowite, jak i bezobjawowe miała obecność przerzutów do węzłów chłonnych miednicy.
    Źródło:
    Ginekologia Onkologiczna; 2007, 5, 4; 218-235
    1731-5379
    Pojawia się w:
    Ginekologia Onkologiczna
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Immunohistochemical characterization of neurons in the vestibular ganglion (Scarpa’s ganglion) of the pig
    Autorzy:
    Dudek, A.
    Sienkiewicz, W.
    Kaleczyc, J.
    Powiązania:
    https://bibliotekanauki.pl/articles/31779.pdf
    Data publikacji:
    2012
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    immunohistochemistry
    neuron
    vestibular ganglion
    pig
    Scarpa’s ganglion zob.vestibular ganglion
    nerve fibre
    Opis:
    The study was carried out on three 4-month old female pigs. All the animals were deeply anesthetized and transcardially perfused with 4% buffered paraformaldehyde (pH 7.4). Vestibular ganglia (VG) were collected and processed for double-labelling immunofluorescence method. The preparations were examined under the Zeiss LSM 710 confocal microscope equipped with adequate filter blocks. Neurons forming VG were round or oval in shape with a round nucleus in the center. The majority of them (58%) were medium (M) (31-50 μm in diameter) while 28 % and 14% were small (S) (up to 30 μm in diameter) or large (L) (above 50 μm in diameter) in size, respectively. Double-labeling immunofluorescence revealed that VG neurons stained for CGRP (approx. 81%; among them 70.5%, 26.2% and 3.3% were M, S and L in size, respectively), VACHT (57%; 63% M, 24% S, 13% L), Met-Enk (25%; 60% M, 12% S, 28% L), VIP (20%; 88% M, 6% S, L), NPY (15%; 67% M, 20% S, 13% L), GAL (15%; 74% M, 21% S, 5% L), SP (12%; 69% M, 25% S, 6% L) and NOS-positive (12%; 50% S, 50% M). The most abundant populations of intraganglionic nerve fibers were those which stained for CGRP or Met-Enk, whereas only single SP- or NOS-positive nerve terminals were observed.
    Źródło:
    Polish Journal of Veterinary Sciences; 2012, 15, 3
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Image analysis of immunohistochemical stains for detection of parathyroid disease
    Autorzy:
    Płaczek, B.
    Orczyk, T.
    Bułdak, R.
    Michalski, M.
    Segiet, O.
    Polaniak, R.
    Powiązania:
    https://bibliotekanauki.pl/articles/333291.pdf
    Data publikacji:
    2015
    Wydawca:
    Uniwersytet Śląski. Wydział Informatyki i Nauki o Materiałach. Instytut Informatyki. Zakład Systemów Komputerowych
    Tematy:
    image processing
    immunohistochemistry
    hyperparathyroidism
    light microscopy
    przetwarzanie obrazu
    immunohistochemia
    nadczynność przytarczyc
    mikroskopia świetlna
    Opis:
    In this paper a method is introduced which enables automatic detection of parathyroid hyperplasia and parathyroid adenoma on the basis of immunohistochemical angiogenesis markers expression in micrographs. The proposed method uses digital image processing techniques and classification algorithms to detect diseased tissue. The disease detection is performed by classification of normalized color intensity histograms. Accuracy of this method was evaluated by using micrographs of parathyroid tissue sections obtained from patients that have undertaken surgery due to primary hyperparathyroidism. Use of different color models, various classifiers, and immunohistochemical markers was considered during the experiments. The experimental results show that the introduced method enables accurate detection of parathyroid disease. The most promising results were obtained for k-nearest neighbor and neural network classifiers.
    Źródło:
    Journal of Medical Informatics & Technologies; 2015, 24; 141-146
    1642-6037
    Pojawia się w:
    Journal of Medical Informatics & Technologies
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    The influence of resiniferatoxin on the chemical coding of neurons in dorsal root ganglia supplying the urinary bladder in the female pig
    Autorzy:
    Bossowska, A.
    Majewski, M.
    Powiązania:
    https://bibliotekanauki.pl/articles/30452.pdf
    Data publikacji:
    2012
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    resiniferatoxin
    chemical coding
    neuron
    dorsal root ganglion
    urinary bladder
    female
    pig
    sensory innervation
    immunohistochemistry
    neuropeptide
    Opis:
    Although resiniferatoxin (RTX) becomes more often used in experimental therapies of sensory system disorders, so far there is no data concerning the influence of RTX on the chemical coding of neurons in dorsal root ganglia (DRG) supplying the urinary bladder in the pig, an animal species considered as a reliable animal model for investigation dealing with human lower urinary tract disorders. Retrograde tracer Fast Blue (FB) was injected into the wall of the right half of the urinary bladder in six juvenile female pigs, and three weeks later, bladder instillation of RTX (500 nmol per animal) was carried out in all the animals. After a week, DRGs were harvested from all the pigs and the neurochemical characterization of FB+ neurons was performed using routine single-immunofluorescence labeling technique on 10-μm-thick cryostat sections. RTX instillation resulted in a distinct decrease in the numbers of FB+ cells containing calcitonin gene-related peptide (CGRP), nitric oxide synthase (NOS), somatostatin (SOM) and calbindin (CB) when compared with those found in the healthy animals (18% vs. 36%, 1% vs. 6%, 0.8% vs. 4% and 0.5% vs. 3%, respectively), and an increase in the number of pituitary adenylate cyclase-activating polypeptide (PACAP)- and galanin (GAL)-immunoreactive (IR) nerve cells (51% vs. 26% and 47% vs. 6.5%). The results obtained suggest that RTX could be taken into consideration when the neuroactive agents are planned to be used in experimental therapies of selected neurogenic bladder illnesses.
    Źródło:
    Polish Journal of Veterinary Sciences; 2012, 15, 1
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Conantokin G-induced changes in the chemical coding of dorsal root ganglion neurons supplying the porcine urinary bladder
    Autorzy:
    Bossowska, A.
    Majewski, M.
    Powiązania:
    https://bibliotekanauki.pl/articles/31315.pdf
    Data publikacji:
    2012
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    conantokin G
    chemical coding
    urinary bladder
    dorsal root ganglion
    neuron
    sensory innervation
    immunohistochemistry
    neuropeptide
    pig
    Opis:
    Conantokin G (CTG), isolated from the venom of the marine cone snail Conus geographus, is an antagonist of N-methyl-d-aspartate receptors (NMDARs), the activation of which, especially those located on the central afferent terminals and dorsal horn neurons, leads to hypersensitivity and pain. Thus, CTG blocking of NMDARs, has an antinociceptive effect, particularly in the case of neurogenic pain treatment. As many urinary bladder disorders are caused by hyperactivity of sensory bladder innervation, it seems useful to estimate the influence of CTG on the plasticity of sensory neurons supplying the organ. Retrograde tracer Fast Blue (FB) was injected into the urinary bladder wall of six juvenile female pigs. Three weeks later, intramural bladder injections of CTG (120 μg per animal) were carried out in all animals. After a week, dorsal root ganglia of interest were harvested from all animals and neurochemical characterization of FB+ neurons was performed using a routine double-immunofluorescence labeling technique on 10-μm-thick cryostat sections. CTG injections led to a significant decrease in the number of FB+ neurons containing substance P (SP), pituitary adenylate cyclase activating polypeptide (PACAP), somatostatin (SOM), calbindin (CB) and nitric oxide synthase (NOS) when compared with healthy animals (20% vs. 45%, 13% vs. 26%, 1.3% vs. 3%, 1.2 vs. 4% and 0.9% vs. 6% respectively) and to an increase in the number of cells immunolabelled for galanin (GAL, 39% vs. 6.5%). These data demonstrated that CTG changed the chemical coding of bladder sensory neurons, thus indicating that CTG could eventually be used in the therapy of selected neurogenic bladder illnesses.
    Źródło:
    Polish Journal of Veterinary Sciences; 2012, 15, 1
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Polish Consensus on Treatment of Gastric Cancer; update 2017
    Autorzy:
    Kulig, Jan
    Wallner, Grzegorz
    Drews, Michał
    Frączek, Mariusz
    Jeziorski, Arkadiusz
    Kielan, Wojciech
    Kołodziejczyk, Piotr
    Nasierowska-Guttmejer, Anna
    Starzyńska, Teresa
    Zinkiewicz, Krzysztof
    Wojtukiewicz, Marek
    Skoczylas, W. Tomasz
    Richter, Piotr
    Krawczyk, Marek
    Powiązania:
    https://bibliotekanauki.pl/articles/1393313.pdf
    Data publikacji:
    2017
    Wydawca:
    Index Copernicus International
    Tematy:
    Gastric Cancer
    Helicobacter pylori
    Laparotomy
    Non-steroidal anti-inflammatory drugs
    Immunohistochemistry
    Surgical treatment
    Panendoscopy
    Biopsy
    Opis:
    The “Polish Research on Gastric Cancer” project has been continued since 1986. The main aim of this project, which is a multicenter and interdisciplinary research, is enhancing the treatment results of gastric cancer patients by developing and promoting the use of optimal methods for diagnosis and treatment, both surgical as well as combined. One of the more important achievements of the project is the development and publication of a document named “Polish Consensus on Treatment of Patients with Gastric Cancer”, whose first version was published in 1998. Following versions were updated adequately to changing trends in the proceedings in patients with gastric cancer. A scientific symposium on “Polish Consensus on Treatment of Gastric Cancer – update 2016” was held in 3-4 June 2016 in Cracow. During the symposium a panel session was held during which all authors publicly presented the Consensus assumptions to be discussed further. Moreover, the already mentioned session was preceded by a correspondence as well as a working meeting in order to consolidate the position. It has to be underlined that the directions and guidelines included in the Consensus are not the arbitrarily assumed rules of conduct in a legal aspect and as such every doctor/team of doctors is entitled to make different decisions as long as they are beneficial to a patient with gastric cancer. The Consensus discusses as follows: a) recommended qualifications (stage of advancement, pathological, lymph node topography and the extent of lymphadenectomy, division of cancer of the gastroesophageal junction), b) rules for diagnostics including recommendations regarding endoscopic examination and clinical evaluation of the advancement stage, c) recommendations regarding surgical treatment (extent of resection, extent of lymphadenectomy, tactics of proceedings in cancer of the gastroesophageal junction), d) recommendations regarding combined treatment with chemotherapy or radiotherapy, e) place of endoscopic and less invasive surgery in the treatment of gastric cancer. This publication is a summary of the arrangements made in the panel session during the abovementioned scientific symposium in Cracow in 2016.
    Źródło:
    Polish Journal of Surgery; 2017, 89, 5; 59-73
    0032-373X
    2299-2847
    Pojawia się w:
    Polish Journal of Surgery
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Metastatic and non-metastatic sentinel inguinofemoral lymph nodes in vulvar cancer show an increased lymphangiogenesis
    Autorzy:
    Piechowicz, M.
    Mikos, M.
    Banas, T.
    Okon, K.
    Pietrus, M.
    Balajewicz-Nowak, M.
    Szczudlik, L.
    Kojs, Z.
    Czerw, A.
    Juszczyk, G.
    Pityński, K.
    Powiązania:
    https://bibliotekanauki.pl/articles/2085422.pdf
    Data publikacji:
    2020
    Wydawca:
    Instytut Medycyny Wsi
    Tematy:
    anti-D2–40 antibody
    lymphatic vessel density
    immunohistochemistry
    sentinel lymph node
    vulvar cancer
    Opis:
    Introduction and objective. Lymph node involvement is a strong predictor of disease recurrence and patient survival in vulvar cancer. The aim of the study was to evaluate the feasibility of sentinel lymph node (SLN) screening, the incidence of skip metastases, and lymph node lymphangiogenesis. Materials and method. Fifty-five patients participated in this prospective, single centre study. A double SLN screening method was employed using radiocolloid (technetium-99 sulfur colloid) and 1.0% Isosulfan Blue. Immunohistochemistry, using a mouse monoclonal antibody against D2–40, was used to evaluate lymphatic vessel density (LVD). All calculations were performed using STATISTICA software v. 10 (StatSoft, USA, 2011); p<0.05 was considered significant. Results. Using both methods of SLN detection, 100% accuracy was achieved, and skip metastases were diagnosed in only one woman (1.82%). Peri-tumour median LVD was significantly increased compared with matched intra-tumour samples (p<0.001), while median LVD was significantly lower in negative, compared with positive SLN, regardless of whether matched non-SLN were negative (p<0.001) or positive (p=0.005). Metastatic SLN exhibited significantly higher median LVD compared with matched negative non-SLN (p=0.015), while no significant difference in median LVD was detected between positive SLN and matched positive non-SLN. However, negative SLN had a significantly higher median LVD compared with matched negative non-SLN (p = 0.012). Conclusions. SLN detection is a safe and feasible procedure in vulvar cancer. In patients without nodular involvement, SLN, compared with non-SLN, exhibited significantly higher median LVD, which may be an indication of its preparation to host metastases, and thus requires further investigation.
    Źródło:
    Annals of Agricultural and Environmental Medicine; 2020, 27, 1; 123-128
    1232-1966
    Pojawia się w:
    Annals of Agricultural and Environmental Medicine
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Immunohistochemiczna metoda identyfikacji komórek CD133+ w glioblastoma
    Immunohistochemical identification methods of CD133+ cells in glioblastoma
    Autorzy:
    Zielonka, Emil
    Powiązania:
    https://bibliotekanauki.pl/articles/1053634.pdf
    Data publikacji:
    2013
    Wydawca:
    Medical Communications
    Tematy:
    CD133
    cancer stem cells
    confocal microscopy
    immunohistochemistry
    glioblastoma
    immunohistochemia
    mikroskopia konfokalna
    nowotworowe komórki macierzyste
    Opis:
    Glioblastoma (GBM, WHO grade IV) is the most lethal type of brain tumours. Despite advances in radiotherapy, chemotherapy and surgical techniques, this tumour is still associated with a median overall survival of approximately 1 year. Histopathological features of GBM include nuclear atypia, foci of palisading necrosis, microvascular proliferation and robust mitotic activity. Glioblastoma is one of the best vascularized tumours in humans and its proliferation is hallmarked by a distinct proliferative vascular component. Studies of glioblastoma’s vascularization have cast some light on the role of non-endothelial cells in tumour neoangiogenesis. A characteristic feature of protein CD133 is its rapid down-regulation during cell differentiation, which makes it a unique cell surface marker for identification of stem cells in brain tissue. CD133+ tumour cells are located mainly in perivascular niches. CD133 positive cells were also found in blood vessels wall. The aim of this study was to optimize immunohistochemical staining method to facilitate identification of cells recognized by anti-CD133 antibody in paraffin-embedded glioblastoma tissue sections. In this study, several pretreatments, detection systems, dilution of antibody and time incubation were used. Immunohistochemical staining method in which autoclave, a buffer pH 9.0 and LSAB+System-HRP were used gave the best result.
    Najczęstszym i jednocześnie najgorzej rokującym nowotworem pochodzenia astrocytarnego jest glioblastoma (IV stopień złośliwości według Światowej Organizacji Zdrowia). Obraz mikroskopowy glioblastoma charakteryzuje się atypią komórkową, obecnością ognisk martwicy palisadowej oraz proliferacją drobnych naczyń. Wyróżniamy dwa typy glioblastoma: pierwotny i wtórny. Jedną z podstawowych cech histopatologicznych tego nowotworu jest obecność proliferacji naczyniowych. Proliferacje naczyń glioblastoma rozpoznawane są jako wielowarstwowe kłębki rozrośniętych i mitotycznie aktywnych komórek endotelialnych. Komórki macierzyste odgrywają znaczącą rolę w tworzeniu sieci naczyń krwionośnych nowotworu za sprawą różnicowania w kierunku komórek endotelialnych. Za marker nowotworowych komórek macierzystych uważa się antygen CD133. Komórki nowotworu wykazujące ekspresję tego białka rozpoznawanego przez odpowiednie przeciwciała zlokalizowane są w niszach otaczających naczynia krwionośne występujące w masie nowotworu. Komórki CD133+ występują również w ścianach naczyń krwionośnych. Celem przeprowadzonych badań było opracowanie optymalnej immunohistochemicznej metody barwienia, z wykorzystaniem przeciwciała przeciw CD133, która ułatwi identyfikację komórek macierzystych. Materiał do badań stanowiły preparaty histopatologiczne pobrane do rutynowych badań histopatologicznych w czasie zabiegu operacyjnego z wcześniej rozpoznanymi przypadkami glioblastoma. Badania obejmowały różne metody barwień immunohistochemicznych z wykorzystaniem zróżnicowanych sposobów odmaskowywania antygenów, z zastosowaniem odmiennych buforów, systemów detekcyjnych oraz rozcieńczeń przeciwciała i czasów inkubacji preparatów z przeciwciałem. Najlepsze wyniki barwienia immunohistochemicznego otrzymano w preparatach, w których w celu odmaskowywania antygenu zastosowano autoklaw, bufor o pH 9,0 i system detekcyjny LSAB+System-HRP.
    Źródło:
    Aktualności Neurologiczne; 2013, 13, 4; 253-257
    1641-9227
    2451-0696
    Pojawia się w:
    Aktualności Neurologiczne
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Prognostic significance of p53 protein accumulation in cancer cells obtained from selected group of patients with sporadic colorectal cancer
    Autorzy:
    Paluszkiewicz, P
    Karski, J.
    Berbec, H.
    Pawlowska-Wakowicz, B.
    Cybulski, M.
    Karski, M.
    Paszkowska, A.
    Powiązania:
    https://bibliotekanauki.pl/articles/2043897.pdf
    Data publikacji:
    1999
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    patient
    cancer cell
    monoclonal antibody
    p53 protein
    cancer prognosis
    immunohistochemistry
    sporadic colorectal cancer
    accumulation
    colorectal cancer
    Źródło:
    Journal of Applied Genetics; 1999, 40, 2; 135-144
    1234-1983
    Pojawia się w:
    Journal of Applied Genetics
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    The expression of androgen receptor in neurons of the anterior pelvic ganglion and celiac-superior mesenteric ganglion in the male pig
    Autorzy:
    Kaleczyc, J.
    Kasica-Jarosz, N.
    Pidsudko, Z.
    Przyborowska, A.
    Sienkiewicz, W.
    Powiązania:
    https://bibliotekanauki.pl/articles/2087598.pdf
    Data publikacji:
    2019
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    androgen receptor
    neurons
    anterior pelvic ganglion
    celiac-superior mesenteric ganglion
    quantitative real-time PCR
    immunohistochemistry
    male pig
    Źródło:
    Polish Journal of Veterinary Sciences; 2019, 1; 151-155
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Comparative studies on bone structure in dairy cows with different feeding conditions
    Autorzy:
    Pilmane, M
    Zitare, I.
    Jemeljanovs, A.
    Powiązania:
    https://bibliotekanauki.pl/articles/30482.pdf
    Data publikacji:
    2010
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    dairy cattle
    cow
    animal feeding
    bone structure
    feeding condition
    histomorphometry
    immunohistochemistry
    bone
    diet
    rapeseed
    dynamic structure
    feed component
    Opis:
    The bone belongs to the dynamic tissues and its structure in domestic cows is still not completely understood in correlation to the impact of different food components. The aim of our work was a histomorphometrical and immunohistochemical research on bone morphology and factors influencing it in healthy dairy cows fed with self-produced grain and with rapeseed cakes. The bone of self-produced grain-fed cows demonstrated statistically significant difference in the number of osteocytes from the bone of rapeseed cakes-fed cows. The rapeseed cakes-fed cows didn't show any bone cell positive for BMP2/4, while FGFR1 increased significantly in their supportive tissues. The number of bFGF- and apoptosis-containing structures varied in cows of both groups. MMP2 expression showed statistically significant difference between both animals' groups with domination in bone of cows fed with self-produced grain. Defensin-, osteopontin- and osteocalcin-contain- ing cells showed tendency to increase in bone of cows on rapeseed cakes diet. Conclusions. The rapeseed-fed cow's long bones demonstrate significant decrease of osteocytes per mm2 and selective increase of FGFR1, suggesting the (compensatory) growth stimulation in supportive tissue. The statistically significant selective absence of MMP2 with a slight tendency of increase in osteopontin and osteocalcin in rapeseed-fed cow's long bones indicates the persistence of seemingly still compensated qualitative changes in bone (beginning of disturbances in mineralization, metabolism etc.) proved also by a slight increase of the bone antimicrobial peptide.
    Źródło:
    Polish Journal of Veterinary Sciences; 2010, 13, 1; 89-95
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Pediatric lymphomatoid papulosis – a case report and management considerations
    Lymphomatoid papulosis w populacji pediatrycznej – opis przypadku i rozważania terapeutyczne
    Autorzy:
    Dec, Michał
    Arasiewicz, Hubert
    Powiązania:
    https://bibliotekanauki.pl/articles/26437917.pdf
    Data publikacji:
    2024-01-30
    Wydawca:
    Śląski Uniwersytet Medyczny w Katowicach
    Tematy:
    lymphomatoid papulosis
    CD30-positive lymphoproliferative disorder
    phototherapy
    immunohistochemistry
    methotrexate
    choroby limfoproliferacyjne z komórek CD30+
    fototerapia
    immunohistochemia
    metotreksat
    Opis:
    Lymphomatoid papulosis (LyP) is a rare cutaneous disorder, most commonly observed in adults, and its occurrence in the pediatric population is exceedingly rare. We present the case of an 6-year-old male patient who exhibited clinical and histopathological features consistent with LyP. The patient presented with multiple erythematous papules on the trunk and extremities, which were accompanied by mild pruritus. The lesions intermittently appeared, disappeared, and changed in morphology. No lymphadenopathy or systemic symptoms were noted. The histopathological examination revealed a dense infiltrate of atypical lymphocytes with cerebriform nuclei in the dermis. Immunohistochemical analysis confirmed CD30 expression in the infiltrating cells, supporting the diagnosis of LyP. Topical corticosteroids were administered to alleviate pruritus and inflammation, although only minimal symptomatic relief was achieved. The beneficial effects of narrowband ultraviolet B (UVB) 311 phototherapy were observed for a duration of four months. Nevertheless, following the cessation of treatment, the reappearance of both the nodular lesions and smaller papular lesions was observed. Consequently, a therapeutic regimen consisting of the administration of methotrexate at a dosage of 10 mg once per week was initiated. The treatment of LyP varies depending on the severity of the lesions and the patient’s symptoms, treatment decisions need to be carefully weighed due to the relatively benign nature of the disease. The diagnosis of LyP in pediatric patients is challenging because of its rarity and potential confusion with malignant lymphomas. Histopathology and immunohistochemistry play a pivotal role in distinguishing LyP from more aggressive entities.
    Lymphomatoid papulosis (LyP) jest rzadką chorobą skóry, najczęściej obserwowaną u dorosłych, a jej występowanie w populacji pediatrycznej jest niezwykle rzadkie. W pracy przedstawiono przypadek 6-letniego dziecka płci męskiej, u którego stwierdzono cechy kliniczne i histopatologiczne zgodne z rozpoznaniem LyP. Na skórze pacjenta obserwowano liczne rumieniowe grudki zlokalizowane na tułowiu i kończynach, a towarzyszył im łagodny świąd. Zmiany chorobowe pojawiały się nawrotowo, następnie ustępowały i zmieniały morfologię. W badaniu przedmiotowym nie stwierdzono limfadenopatii ani objawów ogólnoustrojowych. W badaniu histopatologicznym w skórze właściwej stwierdzono gęsty naciek limfocytów atypowych z hiperchromatycznymi i nieregularnymi jądrami komórkowymi. Analiza immunohistochemiczna potwierdziła ekspresję CD30 w naciekających komórkach, co potwierdza rozpoznanie LyP. W celu złagodzenia świądu i stanu zapalnego podawano miejscowo glikokortykosteroidy, chociaż uzyskano jedynie minimalne złagodzenie objawów. Korzystne efekty fototerapii wąskopasmowej UVB 311 obserwowano przez cztery miesiące. Niemniej jednak po zaprzestaniu leczenia zaobserwowano ponowne pojawienie się zarówno zmian guzkowych, jak i mniejszych zmian grudkowych. W związku z tym rozpoczęto schemat terapeutyczny polegający na podawaniu metotreksatu w dawce 10 mg raz na tydzień. Leczenie LyP różni się zależnie od ciężkości zmian i objawów u pacjenta. Decyzje dotyczące leczenia należy dokładnie rozważyć ze względu na stosunkowo łagodny charakter choroby. Rozpoznanie LyP u dzieci i młodzieży stanowi wyzwanie ze względu na rzadkość występowania choroby i możliwość błędnego rozpoznania tej jednostki chorobowej z chłoniakami złośliwymi. Histopatologia i immunohistochemia odgrywają kluczową rolę w odróżnianiu LyP od bardziej agresywnych jednostek chorobowych.
    Źródło:
    Annales Academiae Medicae Silesiensis; 2024, 78; 24-30
    1734-025X
    Pojawia się w:
    Annales Academiae Medicae Silesiensis
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Density of tumor-associated macrophages (TAMs) and expression of their growth factor receptor MCSF-R and CD14 in canine mammary adenocarcinomas of various grade of malignancy and metastasis
    Autorzy:
    Krol, M.
    Pawlowski, K.
    Majchrzak, K.
    Dolka, I.
    Abramowicz, A.
    Szyszko, K.
    Motyl, T.
    Powiązania:
    https://bibliotekanauki.pl/articles/32107.pdf
    Data publikacji:
    2011
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    adenocarcinoma
    canine adenocarcinoma
    density
    dog
    expression
    growth factor
    immunohistochemistry
    immunology
    malignancy
    mammary adenocarcinoma
    metastasis
    tissue sample
    tumour microenvironment
    tumour-associated macrophage
    Opis:
    Several years ago, the presence of macrophages in the tumor microenvironment was thought to be an inflammatory response to kill the cancer cells. Now, this is clear that the inflammatory cells that exit blood vessels and migrate to the tumor tissue play an important role in cancer progression. Various cells present in the tumor microenvironment enhance cancer growth and invasiveness by secretion of tumor-enhancing products. That is why tumors should not be treated as only aggregates of cancer cells but as separate structures. Macrophages form a major component of the inflammatory infiltration in tumors, where they are termed tumor-associated macrophages (TAMs). To the best of our knowledge, up-to-date there were no studies on tumor associated macrophages and the role of the tumor microenvironment in tumor invasion/metastasis in dogs. This is the first study performed to asses if the number of TAMs and expression of MCSF-R (macrophages colony stimulating factor receptor) and CD14 (LPS co-receptor) are associated with the grade of tumor malignancy and its ability to metastasize. We have performed immunohistochemical analysis of 50 canine mammary adenocarcinomas of various grade of malignancy (1st, 2nd, 3rd) and tumors that gave local or distant metastases. The results indicate that in dogs, similarly to humans and mice, the number of tumor associated macrophages is related to the cancer ability to metastasize. Our results also indicate that the expression of MCSF-R and, what is particularly new finding, CD14 is associated with tumor malignancy and its ability to metastasize. Hence, these molecules play a role in tumor progression, metastasis and microenvironment interactions. These results show that in dogs we should treat the tumor as a whole organ rather than just try to eliminate the cancer cells.
    Źródło:
    Polish Journal of Veterinary Sciences; 2011, 14, 1
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    The influence of botulinum toxin type A (BTX) on the immunohistochemical characteristics of noradrenergic and cholinergic nerve fibres supplying the porcine urinary bladder wall
    Autorzy:
    Lepiarczyk, E.
    Bossowska, A.
    Kaleczyc, J.
    Majewski, M.
    Powiązania:
    https://bibliotekanauki.pl/articles/32489.pdf
    Data publikacji:
    2011
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    botulinic toxin type A
    immunohistochemistry
    noradrenergic nerve fibre
    cholinergic nerve fibre
    nerve fibre
    pig
    urinary bladder
    bladder wall
    botulinum toxin
    neurotoxin
    autonomic neuron
    Opis:
    Botulinum toxin (BTX) belongs to a family of neurotoxins which strongly influence the function of autonomic neurons supplying the urinary bladder. Accordingly, BTX has been used as an effective drug in experimental therapies of a range of neurogenic bladder disorders. However, there is no detailed information dealing with the influence of BTX on the morphological and chemical properties of nerve fibres supplying the urinary bladder wall. Therefore, the present study investigated, using double-labeling immunohistochemistry, the distribution, relative frequency and chemical coding of cholinergic and noradrenergic nerve fibers supplying the wall of the urinary bladder in normal female pigs (n=6) and in the pigs (n=6) after intravesical BTX injections. In the pigs injected with BTX, the number of adrenergic (DβH-positive) nerve fibers distributed in the bladder wall (urothelium, submucosa and muscle coat) was distinctly higher while the number of cholinergic (VAChT-positive) nerve terminals was lower than that found in the control animals. Moreover, the injections of BTX resulted in some changes dealing with the chemical coding of the adrenergic nerve fibers. In contrast to the normal pigs, in BTX injected animals the number of DβH/NPY- or DβH/CGRP-positive axons was higher in the muscle coat, and some fibres distributed in the urothelium and submucosa expressed immunoreactivity to CGRP. The results obtained suggest that the therapeutic effects of BTX on the urinary bladder might be dependent on changes in the distribution and chemical coding of nerve fibers supplying this organ.
    Źródło:
    Polish Journal of Veterinary Sciences; 2011, 14, 2
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Histopathologic and immunohistochemical lesions in liver of mink infected with Aleutian disease virus
    Autorzy:
    Valdovska, A.
    Pilmane, M.
    Powiązania:
    https://bibliotekanauki.pl/articles/30104.pdf
    Data publikacji:
    2011
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    Aleutian disease
    Aleutian mink disease parvovirus
    animal infection
    beta-defensin-2
    histopathology
    immunohistochemistry
    liver
    liver parenchyma
    mink
    nerve growth factor receptor
    metalloproteinase
    Opis:
    Parvovirus of Aleutian disease causes mainly damage to kidneys, but immune complexes deposition and damage may occur also in other organs. In mink farms of Latvia the liver dystrophy or hepatic lipidosis of mink is widely distributed. The goal of this study was to examine probability of liver damage and regeneration of mink infected with Aleutian disease virus. Liver injury was assessed histologically. The mink liver demonstrated inflammation of liver parenchyma and foci of fatty liver. In immunohistochemistry, during liver regeneration the matrix metalloproteinases MMP-9, vascular endothelial growth factor and β-defensin 2 expressions were lower, but MMP-2 and nerve growth factor receptor p75 expression was increased.
    Źródło:
    Polish Journal of Veterinary Sciences; 2011, 14, 1
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Klinicznie nieczynne hormonalnie guzy przysadki
    NFPAs-non-functioning pituitary adenomas
    Autorzy:
    Zawada, Natalia Bożena
    Kunert-Radek, Jolanta
    Powiązania:
    https://bibliotekanauki.pl/articles/1032601.pdf
    Data publikacji:
    2012
    Wydawca:
    Łódzkie Towarzystwo Naukowe
    Tematy:
    klinicznie nieczynne gruczolaki przysadki
    guzy przysadki
    immunohistochemia guzów przysadki
    scyntygrafia receptorowa
    receptory
    dopaminergiczne
    receptory somatostatynowe
    leczenie operacyjne
    radioterapia
    nóż gamma
    analogi somatostatyny
    agoniści dopaminy
    clinically non-functioning pituitary adenomas
    pituitary tumours
    immunohistochemistry of pituitary tumours
    receptor scintigraphy
    dopamine
    receptors
    somatostatin receptors
    surgery
    radiotherapy
    gamma knife
    somatostatin analogues
    dopamine agonists
    Opis:
    The significant progress made in recent years, especially in the field of imaging techniques and immunohistochemistry, enables an earlier diagnosis and the treatment of clinically non-functioning pituitary adenomas (NFPAs). In spite of this, these tumours still remain an important medical issue for a large number of specialities. The aim of this article is to provide an overview of the etiopathogenesis, diagnosis and treatment of clinically non-functioning pituitary adenomas with special reference to the development of highly advanced methods in surgery as well as radiotherapy and, especially, in pharmacotherapy.
    Istotny postęp, jaki dokonał się w ostatnich latach, zwłaszcza w zakresie rozwoju diagnostyki obrazowej i badań immunohistochemicznych, umożliwia wcześniejsze rozpoznawanie, a następnie leczenie nieczynnych hormonalnie gruczolaków przysadki (NFPAs). Mimo to guzy te dalej pozostają istotnym problemem klinicznym dla lekarzy wielu specjalności. Celem pracy jest szczegółowe przedstawienie poglądów na temat etiopatogenezy, obrazu klinicznego, diagnostyki i metod leczenia klinicznie nieczynnych hormonalnie gruczolaków przysadki ze szczególnym uwzględnieniem postępów w technikach chirurgicznych oraz rozszerzających się możliwości radioterapii, a szczególnie farmakoterapii.
    Źródło:
    Folia Medica Lodziensia; 2012, 39, 1; 85-136
    0071-6731
    Pojawia się w:
    Folia Medica Lodziensia
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
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