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Wyświetlanie 1-15 z 106
Tytuł:
Identification of sterile wild oat (Avena sterilis L.) resistance to acetolactate synthase (ALS)-inhibiting herbicides using different assay techniques
Autorzy:
Abdurruhman, A.M.
Uygur, S.
Mathiassen, S.K.
Uygur, N.
Powiązania:
https://bibliotekanauki.pl/articles/2082753.pdf
Data publikacji:
2020
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
acetolactate synthase
agar-based assay
Avena sterilis
Petri-dish assay
whole-plant assay
Opis:
Different techniques have been devised to detect herbicide resistance in weeds, and the over- all aim from this study was to compare four different assay techniques for evaluating acetol- actate synthase (ALS)-inhibiting herbicide resistance in sterile wild oat (Avena sterilis L.). A resistant sterile wild oat population (R) was collected from the wheat field in Kozan, Adana province, Turkey. The susceptible (S) population was collected from the border of the same field. Effects of different doses of mesosulfuron-methyl + iodosulfuron-methyl- -sodium and pyroxsulam + cloquintocet-mexyl were assessed in agar based (seed and seed- ling) assay, Petri dish with seeds, and whole plant pot assay. In the agar based assays, the level of resistance was evaluated by measuring coleoptile and hypocotyl lengths, and sur- vival of seedlings. Plant height and shoot dry weight were measured in the Petri dish and whole plant pot assays, respectively. Results from the dose response analyses showed that both the R and S populations were extremely sensitive to mesosulfuron-methyl + iodosul- furon in the seedling bioassay. The resistance indices (RI’s) of the R biotype treated with mesosulfuron-methyl + iodosulfuron in the agar based seed, Petri dish, and whole plant assays were 2.29, 2.63 and 4.18, respectively. The resistance indices of the R biotype treated with pyroxsulam + cloquintocet-mexyl was 3.41, 5.05 and 2.82 in the agar based seed, Petri dish, and whole plant pot assays, respectively. The agar based seed assays and Petri dish as- say provided feasible, accurate, rapid, and cost effective opportunities to identify resistance in sterile wild oat.
Źródło:
Journal of Plant Protection Research; 2020, 60, 3; 244-252
1427-4345
Pojawia się w:
Journal of Plant Protection Research
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Synthesis and anticancer activity evaluation of some new derivatives of 2-(4-benzoyl-1-piperazinyl)-quinoline and 2-(4-cinnamoyl-1-piperazinyl)-quinoline.
Autorzy:
Kubica, Krzysztof P.
Taciak, Przemyslaw P.
Czajkowska, Agnieszka
Sztokfisz-Ignasiak, Alicja
Wyrebiak, Rafal
Podsadni, Piotr
Mlynarczuk-Bialy, Izabela
Malejczyk, Jacek
Mazurek, Aleksander P.
Powiązania:
https://bibliotekanauki.pl/articles/895388.pdf
Data publikacji:
2018-08-31
Wydawca:
Polskie Towarzystwo Farmaceutyczne
Tematy:
anticancer activity
cytotoxicity assay
2-quinoline
cell migration assay
Opis:
In this study we designed and synthesized twenty new derivatives of 2-(4-benzoyl-1-piperazinyl)-quinoline and 2-(4-cinnamoyl-1-piperazinyl)-quinoline with potential anticancer activity. The structures of synthesized compounds were confirmed by 1H and 13C NMR spectroscopy and MS spectrometry. The activity of novel compounds was evaluated in the cell viability assay as well as in the wound healing assay. Presented data show that examined substances have anticancer activity in cell culture. Seven compounds which showed a high rate of cell growth inhibition were selected for further studies. Three of them strongly reduced growth of B16F10 cells. The novel compounds constitute a good base for further studies and optimization of structure for new therapeutically effective anti-cancerous drugs.
Źródło:
Acta Poloniae Pharmaceutica - Drug Research; 2018, 75, 4; 891-901
0001-6837
2353-5288
Pojawia się w:
Acta Poloniae Pharmaceutica - Drug Research
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
In vitro evaluation of zearalenone toxicity by comet assay
Autorzy:
Harcarova, M.
Conkova, E.
Kolenicova, S.
Holeckova, B.
Proskovcova, M.
Powiązania:
https://bibliotekanauki.pl/articles/16539336.pdf
Data publikacji:
2022
Wydawca:
Polska Akademia Nauk. Czasopisma i Monografie PAN
Tematy:
mycotoxins
toxicity
comet assay
Opis:
The aim of this study was to reveal the potentially genotoxic effect of zearalenone on bovine lymphocytes by comet assay in vitro. The bovine lymphocytes were exposed to various zearalenone concetrations (50; 10; 2; 0.4 and 0.08 ppm). The viability and DNA damage of lymphocytes was monitored after 2 h, 24 h, 48 h and 72 h. After 2 hours of zearalenone exposure, statistically significant DNA damage occurred at all tested concentrations of 0.08 ppm (12.2±1.25; p<0.05), 0.4 ppm (12.7±0.88; p<0.01), 2 ppm (12.0±0.51; p<0.01), 10 ppm (11.2±0.47; p<0.01) and at 50 ppm (14.2±0 61; p<0.001). Significantly greater DNA damage was also found after 24 h, 48 h and 72 h. The obtained results showed that zearalenone may induce DNA damage of the bovine lymphocytes.
Źródło:
Polish Journal of Veterinary Sciences; 2022, 25, 3; 475-477
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Synthesis and anticancer activity evaluation of novel derivatives of 7-amino-4-methylquinolin-2(1H)-one
Autorzy:
Kubica, Krzysztof P.
Taciak, Przemyslaw P.
Czajkowska, Agnieszka
Sztokfisz-Ignasiak, Alicja
Wyrebiak, Rafal
Mlynarczuk-Bialy, Izabela
Malejczyk, Jacek
Mazurek, Aleksander P.
Powiązania:
https://bibliotekanauki.pl/articles/895717.pdf
Data publikacji:
2018-08-31
Wydawca:
Polskie Towarzystwo Farmaceutyczne
Tematy:
anticancer activity
cytotoxicity assay
cell migration assay
quinolin-2(1H)-one
Opis:
In this study we designed and synthesized sixteen new derivatives of 7-amino-4-methylquinolin-2(1H)-one with potential anticancer activity. The structures of synthesized compounds were confirmed by 1H and 13C NMR. The activity of novel substances was evaluated by cell viability assay and wound healing assay. In vitro tests for series of sixteen novel compounds were performed. The results showed that examined compounds are selective for cancer cells, but their activity for various types of cancer is different. Three of new compounds presented ability to inhibit cells migration. The novel compounds constitute a good starting point for further studies and optimization of structure for new therapeutically effective anti-cancerous drugs. Seven compounds, which showed the highest rate of cell inhibition, were selected for further studies.
Źródło:
Acta Poloniae Pharmaceutica - Drug Research; 2018, 75, 4; 903-910
0001-6837
2353-5288
Pojawia się w:
Acta Poloniae Pharmaceutica - Drug Research
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Grupa Wyszehradzka urzędów probierczych. Urząd Probierczy w Budapeszcie
Visegrad Group of Assay Offices. Assay Office in Budapest
Autorzy:
Ulaczyk, Maria Magdalena
Powiązania:
https://bibliotekanauki.pl/articles/2200076.pdf
Data publikacji:
2021
Wydawca:
Główny Urząd Miar
Tematy:
probiernictwo
Grupa Wyszehradzka
Urząd Probierczy w Budapeszcie
assay
Visegrad Group
Assay Office in Budapest
Opis:
Artykuł informuje o współpracy urzędów probierczych, prowadzonej w ramach Grupy Wyszehradzkiej oraz przedstawia, wizytowany niedawno przez pracowników OUP w Warszawie, Urząd Probierczy w Budapeszcie. W artykule wskazano jego specyfikę i odrębności w odniesieniu do polskich urzędów probierczych.
The article informs about the cooperation between assay offices carried out within the Visegrad Group and presents the recently visited by employees of Warsaw Assay Office, Assay Office in Budapest. The article shows its specific and distinctiveness, in comparison to polish assay offices.
Źródło:
Metrologia i Probiernictwo : biuletyn Głównego Urzędu Miar; 2021, 2 (27); 49--58
2300-8806
Pojawia się w:
Metrologia i Probiernictwo : biuletyn Głównego Urzędu Miar
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Detection of alkylation damage in human lymphocyte DNA with the comet assay.
Autorzy:
Collins, Andrew
Dušinská, Mária
Horská, Alexandra
Powiązania:
https://bibliotekanauki.pl/articles/1044089.pdf
Data publikacji:
2001
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
AlkA
DNA damage
comet assay
Opis:
The enzyme 3-methyladenine DNA glycosylase II (AlkA) is a bacterial repair enzyme that acts preferentially at 3-methyladenine residues in DNA, releasing the damaged base. The resulting baseless sugars are alkali-labile, and under the conditions of the alkaline comet assay (single cell gel electrophoresis) they appear as DNA strand breaks. AlkA is not lesion-specific, but has a low activity even with undamaged bases. We have tested the enzyme at different concentrations to find conditions that maximise detection of alkylated bases with minimal attack on normal, undamaged DNA. AlkA detects damage in the DNA of cells treated with low concentrations of methyl methanesulphonate. We also find low background levels of alkylated bases in normal human lymphocytes.Single cell gel electrophoresis (the comet assay) is widely used for the detection of strand breaks in nuclear DNA. It is particularly appropriate for studying the low background levels of damage present in normal human cells, such as peripheral lymphocytes. The cells are embedded in agarose on a microscope slide and lysed with Triton X-100 and 2.5 M NaCl, which remove cytoplasm and most nuclear proteins, but leave the DNA, in supercoiled form, as nucleoids. After incubation in alkali, the DNA is electrophoresed at high pH; DNA is drawn out to form a 'tail' (hence the name 'comet assay') - but only if breaks are present to relax the supercoiling of the nucleoid DNA. In order to increase its sensitivity and selectivity, we have incorporated into the assay an extra step in which the nucleoid DNA is digested with a lesion-specific endonuclease; the additional breaks revealed with this procedure indicate the presence of the particular lesion. So far, endonuclease III (NTH, specific for oxidised pyrimidines) (Collins et al., 1993), formamidopyrimidine DNA glycosylase (FPG, acting on ring-opened purines and the major purine oxidation produce, 8-oxoguanine) (Dušinská & Collins, 1996) and T4 endonuclease V (recognising UV-induced cyclobutane pyrimidine dimers) (Collins et al., 1997b) have been successfully employed. Amongst other things, we have estimated background levels of DNA oxidation (Collins et al., 1997a), and have found this damage to be elevated in human diseases such as diabetes and ankylosing spondylitis (Dušinská et al., 1999).We now report the use of AlkA, a bacterial repair enzyme whose main substrate is 3-methyladenine in DNA, though it also recognises - with lower efficiency - other modified bases (Lindahl, 1993). A recent report (Berdal et al., 1998) suggests that repair enzymes supposedly specific for alkylated bases may in fact create breaks non-selectively (though much less efficiently) at normal bases. Given the size of the genome, even a low efficiency of non-specific breakage could significantly interfere in estimations of background levels of alkylation damage. We reasoned that, by employing a range of concentrations of the enzyme, and carrying out incubations for different lengths of time, we might find a concentration at which only the alkylated bases would be detected, so that the number of breaks would increase to a certain level and then plateau. After optimising reaction conditions, we tested the assay on lymphocytes from different individuals, and also, as a positive control, examined alkylation damage induced by methyl methanesulphonate.
Źródło:
Acta Biochimica Polonica; 2001, 48, 3; 611-614
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Labile iron pool correlates with iron content in the nucleus and the formation of oxidative DNA damage in mouse lymphoma L5178Y cell lines.
Autorzy:
Kruszewski, Marcin
Iwaneńko, Teresa
Powiązania:
https://bibliotekanauki.pl/articles/1043668.pdf
Data publikacji:
2003
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
iron homeostasis
hydrogen peroxide
comet assay
Opis:
Labile iron pool (LIP) constitutes a crossroad of metabolic pathways of iron-containing compounds and is midway between the cellular need for iron, its uptake and storage. In this study we investigated oxidative DNA damage in relation to the labile iron pool in a pair of mouse lymphoma L5178Y (LY) sublines (LY-R and LY-S) differing in sensitivity to hydrogen peroxide. The LY-R cells, which are hydrogen peroxide-sensitive, contain 3 times more labile iron than the hydrogen peroxide-resistant LY-S cells. Using the comet assay, we compared total DNA breakage in the studied cell lines treated with hydrogen peroxide (25 μM for 30 min at 4°C). More DNA damage was found in LY-R cells than in LY-S cells. We also compared the levels of DNA lesions sensitive to specific DNA repair enzymes in both cell lines treated with H2O2. The levels of endonuclease III-sensitive sites and Fapy-DNA glycosylase-sensitive sites were found to be higher in LY-R cells than in LY-S cells. Our data suggest that the sensitivity of LY-R cells to H2O2 is partially caused by the higher yield of oxidative DNA damage, as compared to that in LY-S cells. The critical factor appears to be the availability of transition metal ions that take part in the OH radical-generating Fenton reaction (very likely in the form of LIP).
Źródło:
Acta Biochimica Polonica; 2003, 50, 1; 211-215
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The use of Allium cepa L. assay as bioindicator for the investigation of genotoxic effects of industrial waste water
Autorzy:
Rasgele, Pinar Goc
Powiązania:
https://bibliotekanauki.pl/articles/2073789.pdf
Data publikacji:
2021
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
Allium cepa assay
mitotic index
waste water
Opis:
In this study, genotoxic potential of industrial waste water (IWW) samples was investigated using Allium cepa assay. The root tips were treated with different IWW samples (A, B and C) for 48 hours. The effects of IWW on cytological effects were determined. It was found that all IWW samples significantly increased the percentage of total abnormality. Mitotic chromosomal abnormalities such as irregular metaphase, stickiness, c-mitosis, micronucleus, vagrant chromosomes and bridges were determined. Furthermore, a significant reduction for the mitotic index that is indicative of cellular toxicity was observed in root tips cells, which were treated with IWW samples. A. cepa assay can be used as useful tool for the detection of genotoxic and cytotoxic potential of IWWs.
Źródło:
Archives of Environmental Protection; 2021, 47, 4; 3--8
2083-4772
2083-4810
Pojawia się w:
Archives of Environmental Protection
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Potentiometric determination of cysteine with thiol sensitive silver-mercury electrode
Autorzy:
Drożdż, Ryszard
Naskalski, Jerzy
Ząbek-Adamska, Anna
Powiązania:
https://bibliotekanauki.pl/articles/1041141.pdf
Data publikacji:
2007
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
potentiometry
cysteine
real time assay
homocysteine
glutathione
Opis:
A potentiometric procedure for cysteine thiol group concentration monitoring in media generating free radicals was developed using a thiol specific silver-mercury electrode. Electrolytic deposition of mercury on a silver wire and treatment with 20 mM cysteine in 0.5 M NaOH were used to produce the electrode. A silver-chloride electrode in saturated KCl was the reference. A glass capillary with 1 M KNO3 in 1% agarose gel was the liquid junction. The electrode responded to cysteine concentration in the range from 0.01 to 20 mM yielding a perfect linear relationship for the dependence of log [cysteine] versus electrode potential [mV], with b0 (constant) = -373.43 [mV], b1 (slope) = -53.82 and correlation coefficient r2 = 0.97. The electrode potential change per decade of cysteine concentration was 57 mV. The minimal measurable signal response was at a cysteine concentration of 0.01 mM. The signal CV amounted to 4-6% for cysteine concentrations of 0.01 to 0.05 mM and to less than 1% for cysteine concentrations of 0.5 to 20 mM. The response time ranged from about 100 s for cysteine concentrations of 0.01 to 0.1 mM to 30 s at higher cysteine concentrations. The standard curve reproducibility was the best at cysteine concentrations from 0.1 to 20 mM. In a reaction medium containing cysteine and copper(II)-histidine complex ([His-Cu]2+) solution in 55 mM phosphate buffer pH 7.4 the electrode adequately responded to changes in cysteine concentration. Beside cysteine, the silver-mercury electrode responded also to thiol groups of homocysteine and glutathione, however, the Nernst equation slope was about half of that for cysteine.
Źródło:
Acta Biochimica Polonica; 2007, 54, 1; 205-211
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Dose and Time Dependent Response of HL - 60 Cells Against Newly Isolated Phenolic Compound from Pandanus odoratissimus
Autorzy:
Kamble, Abhaykumar
Reddy, Chinnappa
Kulkarni, Amitkumar
Powiązania:
https://bibliotekanauki.pl/articles/1194031.pdf
Data publikacji:
2015
Wydawca:
Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
Tematy:
Cytotoxicity
HL- 60
MTT assay
Phenolic compound
Opis:
Bioassay guided chromatographic purification of methanolic extract of Pandanus odoratissium leaves afforded new phenolic compound. The isolated compound was identified as 4-(4-(3,4-dimethoxyphenyl) hexahyddrofuro[3,4-c]furan-l-yl)-2-methoxyphenyl acetate by the spectroscope methods. The puropose of this investigation was to check the effect of the phenolic compound 4-(4-(3, 4-dimethoxyphenyl)hexahyddrofuro[3,4-c]furan-l-yl)-2-methoxyphenyl acetate isolated pure compound, the human leukemia (HL-60) cell line as model to evaluate the cytoxicity of compound on the MTT assay. The results obtained from the parameter indicates the compound has significantly reduced the viability of HL-60 cells shows LD50 values of 21.24 ± 0.5 μg/mL, 10.25 ± 0.3 μg/mL and 5.2 ± 0.6 μg/mL, upon 12, 24 and 48 hours of exposure respective indicates a dose and dependent response relationship. The present study indicate the compound is highly cytotoxic to human leukemia (HL-60) cells supports it as an effective therapeutic agent against leukemia.
Źródło:
World Scientific News; 2015, 10; 44-58
2392-2192
Pojawia się w:
World Scientific News
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Some substances present in fungal protein preparations as interfering factors in the determination of protein by the Lowry method
Wpływ wybranych substancji występujących w grzybowych preparatach białkowych na wyniki oznaczeń zawartości białka metodą Lowryego
Autorzy:
Czajkowska, D.
Wiącek-Żychlińska, A.
Jędrychowska, B.
Sawicka-Żukowska, R.
Powiązania:
https://bibliotekanauki.pl/articles/1398775.pdf
Data publikacji:
1986
Wydawca:
Instytut Rozrodu Zwierząt i Badań Żywności Polskiej Akademii Nauk w Olsztynie
Tematy:
protein assay
colorimetric method
fungal protein preparation
Opis:
Study was made of the interference with the protein determination using the Lowry procedure, caused by starch, pectin, carboxymethylcellulose and of their enzymie degradation products as well as of other substances contained in the media used for the production of fungal protein preparations. The interfering effect of these substances was investigated prior to and after their pretreatment with 1 M NaOH at 100°C for 10 min. It was found that the investigated substances affect the protein results in a different measure, and that their pretreatment enhances and sometimes reverses the interfering action.
Prześledzono wpływ skrobi, pektyny, celulozy i produktów ich rozkładu enzymatycznego oraz innych związków chemicznych używanych jako składniki podłóż i występujących w grzybowych preparatach białkowych, na wyniki oznaczeń zawartości białka metodą Lowry'ego, zaproponowaną przez Raimbaulta i Alazarda (18) Wpływ wyżej wymienionych substancji określano bez i po wstępnym traktowaniu roztworem 1 M NaOH w temp. 100°C przez 10 min. Stwierdzono, że skrobia i dekstryny (rys. 2), karboksymetyloceluloza i sacharoza (rys. 4) oraz mocznik (rys. 3), nie traktowane wstępnie gorącym roztworem NaOH, w stężeniach do 1% nie wpływają na wyniki oznaczeń zawartości białka metodą Lowry'ego. Większość spośród przebadanych soli mineralnych, w stężeniach powyżej 1%, zaniża w różnym stopniu odczyty ilości białka badaną metodą (rys. 5). Glukoza, galaktoza i maltoza w stężeniach powyżej 0,1% (rys. 4) oraz pektyna i produkty jej enzymatycznego rozkładu w stężeniach wyższych niż odpowiednio 0,45 i 0,3%, nie traktowane wstępnie gorącym roztworem 1 M NaOH, (rys. 3) zawyżają odczyty absorbancji. Wstępne traktowanie roztworem NaOH w temp. 100°C przez 10 min wyżej wymienionych substancji organicznych na ogół potęguje i często zmienia ich wpływ na odczyty zawartości białka w próbce. Na przykład glukoza, galaktoza i maltoza, poddane wstępnemu ogrzewaniu w roztworze NaOH, zawyżają odczyty absorbancji począwszy już od stężenia 0,02% (rys. 4), natomiast mocznik począwszy od tego samego stężenia (0,02%) wyraźnie zaniża wyniki oznaczeń zawartości białka (rys. 3). W związku z dużym rozcieńczaniem próbek przed wykonaniem oznaczenia (od 1000 do 2000 razy) a tym samym znacznym zmniejszaniem się stężenia substancji towarzyszących, mogących wpływać na wyniki analizy, metoda Lowry'ego zaproponowana przez Raimbault a i Alazard a (18) jest przydatna i pozwala uzyskiwać porównywalne wyniki zawartości białka dla większości grzybowych preparatów białkowych. W niektórych jednak przypadkach, na przykład przy stosowaniu podłóż zawierających znaczne ilości słomy i/lub wytłoków jabłkowych oraz wysłoków buraczanych obserwuje się wyraźne zawyżenie wyników oznaczeń białka, co wskazuje na konieczność zmodyfikowania metody (np. uwzględnianie odpowiednich poprawek).
Źródło:
Acta Alimentaria Polonica; 1986, 12, 2; 135-142
0137-1495
Pojawia się w:
Acta Alimentaria Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Niedotlenienie guza jako czynnik predykcyjny w radioterapii onkologicznej
Tumour hypoxia as a predictive factor for radiotherapy
Autorzy:
Martenka, Piotr
Roszak, Andrzej
Powiązania:
https://bibliotekanauki.pl/articles/908212.pdf
Data publikacji:
2006
Wydawca:
Medical Communications
Tematy:
anemia
cervix carcinoma
hypoxia
predictive assay
radiotherapy
Opis:
Radiotherapy is, next to surgery, the basic method of treatment in oncology. The intensive searching of the predictive factors for radiotherapy effectiveness, are still continuing. The mechanism of radiotherapy action depends on the cellular oxygen presence. The latest studies evidence, that the molecular oxygen, through the mechanism of fixation, leads to destruction of the DNA damage, caused by the ionizing radiation, repair. Number of studies has demonstrated, that the tumour hypoxia evaluation correlates with radiotherapy effectiveness. However, the universal method assessing the tumour hypoxia does not exist. Actually used methods, as Eppendorf’s electrode, the molecular markers of hypoxia evaluation, or PET studies with oxydo-reductive radiopharmaceutical agents, bring conflicting results. So, a lot of doubts about the quantitative and qualitative character of relationship between hypoxia and sensitivity for radiotherapy still exist. Moreover, the analysis is impeded by fact, that the hypoxia has significant negative prognostic influence on the treatment results with radiotherapy, through the genome and proteomic changes induction. The paper presents the actual status of knowledge about the influence of tumour hypoxia on radiotherapy effectiveness.
Radioterapia jest obok chirurgii podstawową metodą leczenia nowotworów. Trwają obecnie intensywne poszukiwania czynników predykcyjnych dla skuteczności radioterapii. Mechanizm działania radioterapii zależny jest od obecności tlenu w komórce. Najnowsze doświadczenia dowodzą, że tlen cząsteczkowy poprzez mechanizm fiksacji zaburza naprawę uszkodzeń wywołanych w DNA promieniowaniem jonizującym. Liczne badania wykazały, że ocena niedotlenienia guza koreluje z wynikiem radioterapii. Brak jednak uniwersalnej metody oceniającej hipoksję. Aktualnie stosowane metody, jak pomiar elektrodą Eppendorfa, ocena markerów molekularnych hipoksji czy badania PET z użyciem oksydoredukcyjnych radiofarmaceutyków dostarczają sprzecznych wyników. Istnieje więc wiele wątpliwości co do ilościowego i jakościowego charakteru zależności między niedotlenieniem a wrażliwością na radioterapię. Ponadto analizę utrudnia fakt, że niedotlenienie wywiera znaczny negatywny prognostyczny wpływ na wyniki leczenia radioterapią poprzez indukcję zmian genomowych i proteomicznych. W pracy przedstawiono aktualny stan wiedzy na temat wpływu niedotlenienia guza na skuteczność radioterapii.
Źródło:
Ginekologia Onkologiczna; 2006, 4, 2; 99-107
1731-5379
Pojawia się w:
Ginekologia Onkologiczna
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Laboratory automatic measuring system for gamma specimens
Autorzy:
Jakowiuk, A.
Filipiak, P.
Bartak, J.
Pieńkos, P.
Kowalska, E.
Powiązania:
https://bibliotekanauki.pl/articles/146868.pdf
Data publikacji:
2012
Wydawca:
Instytut Chemii i Techniki Jądrowej
Tematy:
gamma counter
radioimmunoassay (RIA)
immunoradiometric assay (IRMA)
Opis:
LG Gamma Counter is designed for low activity measurement of the radioactive isotope iodine 125I in liquid or solid samples during radioimmunoassay (RIA) and immunoradiometric assay (IRMA) in small or medium size clinic laboratories. Apart from RIA and IRMA programmed procedures, a measurement of small activity gamma samples is possible. The well scintillator NaI(Tl) is used as radiation detector for the isotope 125 I in the measuring channel, the well dimensions fi being 17 x 38 mm. Gamma Counter was adapted for measuring radioactivity of single samples. The counter is equipped with an automatic system allowing transportation of examined samples to a scintillator well. The gauge is based on an integrated computer which is working under the control of Windows CE and is equipped with special software allowing for setting measurement parameters and communication with an external computer. The measured sample is placed inside the scintillator well either by an automatic sample feeder or manually. The pulses from a photomultiplier tube after amplification and shaping in pulse amplifiers are counted in the window of a single channel analyser by a programmable pulse counter under the control of microprocessor system. The measured count rate is the measure of activity of the assayed sample. Expected count rate error due to unstable operation of amplification is lower than 1%.
Źródło:
Nukleonika; 2012, 57, 4; 633-635
0029-5922
1508-5791
Pojawia się w:
Nukleonika
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Isolation of Proteins From Potato Tubers
Autorzy:
Murawska, Zofia
Dębski, Janusz
Szajko, Katarzyna
Lebecka, Renata
Powiązania:
https://bibliotekanauki.pl/articles/2199680.pdf
Data publikacji:
2017-06-20
Wydawca:
Instytut Hodowli i Aklimatyzacji Roślin
Tematy:
bicinchoninic acid assay
mass spectrometry
tuber proteomes
Opis:
Here we optimized an efficient and reproducible method for proteins isolation from potato tubers for quan-titative proteomic analysis, aimed at detection of differentially expressed proteins upon various experimental conditions.
Źródło:
Plant Breeding and Seed Science; 2017, 75; 23-27
1429-3862
2083-599X
Pojawia się w:
Plant Breeding and Seed Science
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Resistance of potato tubers to Phytophthora infestans evaluated in laboratory tests and field trials
Autorzy:
Rogozina, Elena V.
Patrikeyeva, Maria V.
Powiązania:
https://bibliotekanauki.pl/articles/2198858.pdf
Data publikacji:
2004-12-20
Wydawca:
Instytut Hodowli i Aklimatyzacji Roślin
Tematy:
advanced potato clone
field trial
Phytophthora infestans
tetraploid interspecific hybrid
tuber resistance
tuber slices assay
whole tubers assay
Opis:
Thirty potato genotypes of different maturity were evaluated for tuber blight resistance both under laboratory and field conditions. From 2000 to 2003 replicated experiments were conducted with three potato cultivars registered in Russia and 27 tetraploid interspecific hybrids that were generated at VIR. Each genotype was assessed twice using laboratory tests, and its resistance was evaluated every year in a field trial. The results showed that four hybrid clones: 88-2, 95-23-3, 97-152-6 and 97-162-5 exhibited a superior performance of tuber resistance to Phytophthora infestans both in laboratory tests and field trials. Two cultivars and 10 hybrid clones were found susceptible to infection in all tests. Significant differences between the remaining 14 genotypes in tuber slice resistance, whole tuber resistance and the expression of resistance to tuber blight in the field were observed.
Źródło:
Plant Breeding and Seed Science; 2004, 50; 147-154
1429-3862
2083-599X
Pojawia się w:
Plant Breeding and Seed Science
Dostawca treści:
Biblioteka Nauki
Artykuł
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