Dose and Time Dependent Response of HL - 60 Cells Against Newly Isolated Phenolic Compound from Pandanus odoratissimus

Bioassay guided chromatographic purification of methanolic extract of Pandanus odoratissium leaves afforded new phenolic compound. The isolated compound was identified as 4-(4-(3,4-dimethoxyphenyl) hexahyddrofuro[3,4-c]furan-l-yl)-2-methoxyphenyl acetate by the spectroscope methods. The puropose of this investigation was to check the effect of the phenolic compound 4-(4-(3, 4-dimethoxyphenyl)hexahyddrofuro[3,4-c]furan-l-yl)-2-methoxyphenyl acetate isolated pure compound, the human leukemia (HL-60) cell line as model to evaluate the cytoxicity of compound on the MTT assay. The results obtained from the parameter indicates the compound has significantly reduced the viability of HL-60 cells shows LD50 values of 21.24 ± 0.5 μg/mL, 10.25 ± 0.3 μg/mL and 5.2 ± 0.6 μg/mL, upon 12, 24 and 48 hours of exposure respective indicates a dose and dependent response relationship. The present study indicate the compound is highly cytotoxic to human leukemia (HL-60) cells supports it as an effective therapeutic agent against leukemia.