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    Tytuł:
    Kinetics of a nucleoside release from lactide-caprolactone and lactide-glycolide polymers in vitro.
    Autorzy:
    Kryczka, Tomasz
    Grieb, Paweł
    Bero, Maciej
    Kasperczyk, Janusz
    Dobrzynski, Piotr
    Powiązania:
    https://bibliotekanauki.pl/articles/1044392.pdf
    Data publikacji:
    2000
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    biodegradable polymers
    adenosine
    Opis:
    We assessed the rate of release of a model nucleoside (adenosine, 5%, w/w) from nine different lactide-glycolide or lactide-caprolactone polymers. The polymer discs were eluted every second day with an artificial cerebrospinal fluid at the elution rate roughly approximating the brain extracellular fluid formation rate. Adenosine in eluate samples was assayed by HPLC. Three polymers exhibited a relatively constant release of adenosine for over four weeks, resulting in micromolar concentrations of nucleoside in the eluate. This points to the neccessity of further development of polymers of this types as intracerebral nucleoside delivery systems for local treatment of brain tumors.
    Źródło:
    Acta Biochimica Polonica; 2000, 47, 1; 59-64
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Synteza analogów adenozyny
    Synthesis of adenosine analogues
    Autorzy:
    Samsel, M.
    Dzierzbicka, K.
    Powiązania:
    https://bibliotekanauki.pl/articles/172542.pdf
    Data publikacji:
    2012
    Wydawca:
    Polskie Towarzystwo Chemiczne
    Tematy:
    adenozyna
    receptory dla adenozyny
    analogi adenozyny
    aktywność biologiczna
    adenosine
    adenosine receptors
    synthesis
    adenosine analogues
    biological activity
    Opis:
    Adenosine (Rys. 1) is a purine nucleoside playing an important role in human body. It is involved in key pathways such as purinergic nucleic acid base synthesis, amino acid metabolism and modulation of cellular metabolic status [1,2]. Adenosine acts through the four types of adenosine receptors: A1, A2A, A2B and A3 belonging to the G protein-coupled receptor family [3]. In physiological conditions this nucleoside is present in a micromolar range [5]. However, when metabolic stress occurs extracellular level of adenosine raises revealing its protective properties. Depending on an activated receptor subtype, adenosine demonstrates cardioprotective and neuroprotective activity during hypoxia or ischemia, it stimulates the immunological system [6, 7]. Besides many potential applications, adenosine is used mainly for the treatment of paroxysmal supraventricular tachycardia. Limitations are linked to a very short blood half-time and no receptor specificity [8]. This review is focused on novel literature data about synthesis of adenosine analogues with interesting biological activities. In order to influence adenosine receptor selectivity and pharmacokinetic properties a nucleoside structure can be modified in purine [14, 15, 17, 22, 26, 27, 35] or sugar ring [29, 32]. New interesting compounds are also synthesized by cyclisation of adenosine [36]. Modification of adenosine structure allowed obtaining compounds with targeted action: antiarrhythmic [11, 12], antinociceptive [9], antilipolytic [13], antiviral [29] or anticancer [35].
    Źródło:
    Wiadomości Chemiczne; 2012, 66, 3-4; 321-340
    0043-5104
    2300-0295
    Pojawia się w:
    Wiadomości Chemiczne
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Effect of insulin and glucose on adenosine metabolizing enzymes in human B lymphocytes
    Autorzy:
    Kocbuch, Katarzyna
    Sakowicz-Burkiewicz, Monika
    Grden, Marzena
    Szutowicz, Andrzej
    Pawelczyk, Tadeusz
    Powiązania:
    https://bibliotekanauki.pl/articles/1040536.pdf
    Data publikacji:
    2009
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    glucose
    insulin
    B lymphocytes
    adenosine kinase
    adenosine deaminase
    5'-nucleotidase
    Opis:
    In diabetes several aspects of immunity are altered, including the immunomodulatory action of adenosine. Our study was undertaken to investigate the effect of different glucose and insulin concentrations on activities of adenosine metabolizing enzymes in human B lymphocytes line SKW 6.4. The activity of adenosine deaminase in the cytosolic fraction was very low and was not affected by different glucose concentration, but in the membrane fraction of cells cultured with 25 mM glucose it was decreased by about 35% comparing to the activity in cells maintained in 5 mM glucose, irrespective of insulin concentration. The activities of 5'-nucleotidase (5'-NT) and ecto-5'-NT in SKW 6.4 cells depended on insulin concentration, but not on glucose. Cells cultured with 10-8 M insulin displayed an about 60% lower activity of cytosolic 5'-NT comparing to cells maintained at 10-11 M insulin. The activity of ecto-5'-NT was decreased by about 70% in cells cultured with 10-8 M insulin comparing to cells grown in 10-11 M insulin. Neither insulin nor glucose had an effect on adenosine kinase (AK) activity in SKW 6.4 cells or in human B cells isolated from peripheral blood. The extracellular level of adenosine and inosine during accelerated catabolism of cellular ATP depended on glucose, but not on insulin concentration. Concluding, our study demonstrates that glucose and insulin differentially affect the activities of adenosine metabolizing enzymes in human B lymphocytes, but changes in those activities do not correlate with the adenosine level in cell media during accelerated ATP catabolism, implying that nucleoside transport is the primary factor determining the extracellular level of adenosine.
    Źródło:
    Acta Biochimica Polonica; 2009, 56, 3; 439-446
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Purinergic signaling in B cells
    Autorzy:
    Przybyła, Tomasz
    Sakowicz-Burkiewicz, Monika
    Pawełczyk, Tadeusz
    Powiązania:
    https://bibliotekanauki.pl/articles/1038516.pdf
    Data publikacji:
    2018
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    B cells
    purinergic signaling
    adenosine
    adenosine triphosphate
    P1 receptors
    P2 receptors
    Opis:
    Adenosine and adenosine triphosphate are involved in purinergic signaling which plays an important role in control of the immune system. Much data have been obtained regarding impact of purinergic signaling on dendritic cells, macrophages, monocytes and T lymphocytes, however less attention has been paid to purinergic regulation of B cells. This review summarizes present knowledge on ATP- and Ado-dependent signaling in B lymphocytes. Human B cells have been shown to express A1-AR, A2A-AR, A2B-AR and A3-AR and each subtype of P2 receptors. Surface of B cells exhibits two antagonistic ectoenzymatic pathways, one relies on constitutive secretion and resynthesis of ATP, while the second one depends on degradation of adenosine nucleotides to nucleosides and their subsequent degradation. Inactivated B cells remain under the suppressive impact of autocrine and paracrine Ado, whereas activated B lymphocytes increase ATP release and production. ATP protects B cells from Ado-induced suppression and exerts pro-inflammatory effect on the target tissues, and it is also involved in the IgM release. On the other hand, Ado synthesis is necessary for optimal development, implantation and maintenance of the plasmocyte population in bone marrow in the course of the primary immune response. Moreover, Ado plays an important role in immunoglobulin class switching, which is a key mechanism of humoral immune response. Disruption of purinergic signaling leads to severe disorders. Impairment of Ado metabolism is one of the factors responsible for common variable immunodeficiency. There are several lines of evidence that dysfunction of the immune system observed during diabetes may in part depend on disrupted ATP and Ado metabolism in the B cells.
    Źródło:
    Acta Biochimica Polonica; 2018, 65, 1; 1-7
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Adenosine as a metabolic regulator of tissue function: production of adenosine by cytoplasmic 5-nucleotidases
    Autorzy:
    Borowiec, Agnieszka
    Lechward, Katarzyna
    Tkacz-Stachowska, Kinga
    Składanowski, Andrzej
    Powiązania:
    https://bibliotekanauki.pl/articles/1041236.pdf
    Data publikacji:
    2006
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    5'-nucleotidase
    adenosine
    metabolic regulation
    Opis:
    Adenosine is a product of complete dephosphorylation of adenine nucleotides which takes place in various compartments of the cell. This nucleoside is a significant signal molecule engaged in regulation of physiology and modulation of the function of numerous cell types (i.e. neurons, platelets, neutrophils, mast cells and smooth muscle cells in bronchi and vasculature, myocytes etc.). As part a of purinergic signaling system, adenosine mediates neurotransmission, conduction, secretion, vasodilation, proliferation and cell death. Most of the effects of adenosine help to protect cells and tissues during stress conditions such as ischemia or anoxia. Adenosine receptors and nucleoside transporters are targets for potential drugs in many pathophysiological situations. The adenosine-producing system in vertebrates involves a cascade dephosphorylating ATP and ending with 5'-nucleotidase (EC 3.1.3.5) localized either on the membrane or inside the cell. In this paper the cytoplasmic variants of 5'-nucleotidase are broadly characterized as well as their clinical relevance. The role of AMP-selective 5'-nucleotidase (cN-I) in the heart, skeletal muscle and brain is highlighted. cN-I action is crucial during ischemia and important for the efficacy of some nucleoside-based drugs and in the regulation of the substrate pool for nucleic acids synthesis. Inhibitors used in studying the roles of cytoplasmic and membrane-bound 5'-nucleotidases are also described.
    Źródło:
    Acta Biochimica Polonica; 2006, 53, 2; 269-278
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Inhibition study of adenosine deaminase by caffeine using spectroscopy and isothermal titration calorimetry.
    Autorzy:
    Saboury, A
    Divsalar, A
    Ataie, G
    Amanlou, M
    Moosavi-Movahedi, A
    Hakimelahi, G
    Powiązania:
    https://bibliotekanauki.pl/articles/1043464.pdf
    Data publikacji:
    2003
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    calorimetry
    inhibition
    caffeine
    adenosine deaminase
    Opis:
    Kinetic and thermodynamic studies were made on the effect of caffeine on the activity of adenosine deaminase in 50 mM sodium phosphate buffer, pH 7.5, using UV spectrophotometry and isothermal titration calorimetry (ITC). An uncompetitive inhibition was observed for caffeine. A graphical fitting method was used for determination of binding constant and enthalpy of inhibitor binding by using isothermal titration microcalorimetry data. The dissociation-binding constant is equal to 350 μM by the microcalorimetry method, which agrees well with the value of 342 μM for the inhibition constant that was obtained from the spectroscopy method. Positive dependence of caffeine binding on temperature indicates a hydrophobic interaction.
    Źródło:
    Acta Biochimica Polonica; 2003, 50, 3; 849-855
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Czy pochodne nukleozydów i nukleotydów mogą w przyszłości stać się skutecznymi lekami przeciwpadaczkowymi?
    Can nucleoside and nucleotide precursors become future successful anti-epileptic drugs?
    Autorzy:
    Cieślak, Marek
    Komoszyński, Michał
    Powiązania:
    https://bibliotekanauki.pl/articles/1058218.pdf
    Data publikacji:
    2010
    Wydawca:
    Medical Communications
    Tematy:
    adenosine
    adenosine receptors
    epileptic seizure
    guanosine
    neuroprotection
    napad drgawkowy
    neuroprotekcja
    adenozyna
    guanozyna
    receptory adenozynowe
    Opis:
    Many examples of experimental epilepsy show that epileptic seizures occur due to release of stimulatory neurotransmitters into intracellular spaces. In CNS adenosine suppresses exocytosis of glutamate and asparginate but guanosine increases the reverse uptake of glutamate by astrocytes and thus lowers it concentration outside the cell. In this process both nucleosides participate in suppressing the epileptic seizures. By decreasing concentration of ectoadenosine and ectoguanosine outside the cell, that compounds can protect neurons from cellular degeneration. It was shown in many animal models for experimental epilepsy that adenosine A1 and A2A receptors were involved in the process of stopping the seizures. Moreover, some of the conventional anti-epileptic drugs reveal enhance their therapeutic abilities by interactions with the adenosine receptors, being either agonists or antagonists. These interactions modulate the activity of receptors and consequently regulate the neuroprotection processes. Some agonists of adenosine receptors increase the epileptic episodes reaction to those compounds. Anti-episode action of adenosine and guanosine as well as agonists and antagonists of nucleoside receptors indicate the possibility of applying the knowledge about these processes towards production of new anti-epileptic medication. Successful anti-epileptic medication may be based on compounds that have the ability to increase the concentration of ectoadenosine i.e; adenosine deaminase inhibitors, adenosine kinase inhibitors or compounds with ability to suppress reverse uptake of nucleosides. Another method to increase the concentration of extracellular adenosine is to increase the activity of 5’-nucleotidase. That in effect will increase the amount of ectoadenosine by degradation of ectoAMP. There are very promising results revealed that oral administration of guanosine and GMP as well as guanosine by itself given intraperitoneally and intraventricularly what halted epileptic seizures caused by quinolinic acid which is a glutamate agonist.
    Napady drgawkowe są wynikiem uwalniania neurotransmiterów pobudzających do przestrzeni pozakomórkowej. W ośrodkowym układzie nerwowym ektoadenozyna hamuje egzocytozę glutaminianu i asparaginianu, natomiast ektoguanozyna, zwiększając wychwyt zwrotny glutaminianu przez astrocyty, obniża jego stężenie poza komórką. W ten sposób oba nukleozydy uczestniczą w hamowaniu napadu drgawkowego. Nukleozydy te, obniżając stężenie powyższych neurotransmiterów poza komórką, chronią neurony przed śmiercią, pełnią więc funkcję neuroprotekcyjną. W różnych modelach zwierzęcych padaczek eksperymentalnych wykazano, że w przerwaniu napadu drgawkowego uczestniczą receptory adenozynowe A1 i A2A. Ma miejsce współdziałanie leków przeciwpadaczkowych i receptorów adenozynowych, bowiem niektóre z nich, takie jak karbamazepina, działają za pośrednictwem receptorów adenozynowych A1, a niektórzy agoniści receptorów A1 potęgują działanie przeciwdrgawkowe tych leków. Przeciwdrgawkowe działanie adenozyny i guanozyny oraz agonistów i antagonistów receptorów nukleozydowych wskazuje na możliwość wykorzystania wiedzy o tych procesach w projektowaniu nowych leków przeciwpadaczkowych. Skutecznymi lekami przeciwdrgawkowymi mogą okazać się związki zwiększające stężenie ektoadenozyny, takie jak: inhibitory deaminazy adenozyny, kinazy adenozynowej oraz związki hamujące wychwyt zwrotny nukleozydów. Innym sposobem zwiększenia stężenia pozakomórkowej adenozyny jest wzrost aktywności 5’-nukleotydazy powiększającej pulę ektoadenozyny przez degradację ektoAMP. Obiecujące są również rezultaty doustnego podania guanozyny i GMP, a także samej guanozyny podanej dokomorowo i dootrzewnowo, które powodowało przerywanie drgawek wywoływanych przez agonistę glutaminianu – kwas chinolinowy.
    Źródło:
    Aktualności Neurologiczne; 2010, 10, 4; 206-212
    1641-9227
    2451-0696
    Pojawia się w:
    Aktualności Neurologiczne
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Czy pochodne nukleozydów i nukleotydów mogą w przyszłości stać się skutecznymi lekami przeciwpadaczkowymi?
    Can nucleoside and nucleotide precursors become future successful anti-epileptic drugs?
    Autorzy:
    Ciślak, Marek
    Komoszyński, Michał
    Powiązania:
    https://bibliotekanauki.pl/articles/1061366.pdf
    Data publikacji:
    2006
    Wydawca:
    Medical Communications
    Tematy:
    adenosine
    adenosine receptors
    epileptic seizure
    guanosine
    neuroprotection
    neuroprotekcja
    receptory adenozynowe
    adenozyna
    guanozyna
    napad drgawkowy
    Opis:
    Many examples of experimental epilepsy show that epileptic seizures occur due to release of stimulatory neurotransmitters into intracellular spaces. In CNS adenosine suppresses exocytosis of glutamate and asparginate but guanosine increases the reverse uptake of glutamate by astrocytes and thus lowers it concentration outside the cell. In this process both nucleosides participate in suppressing the epileptic seizures. By decreasing concentration of ectoadenosine and ectoguanosine outside the cell, that compounds can protect neurons from cellular degeneration. It was shown in many animal models for experimental epilepsy that adenosine A1 and A2A receptors were involved in the process of stopping the seizures. Moreover, some of the conventional anti-epileptic drugs reveal enhance their therapeutic abilities by interactions with the adenosine receptors, being either agonists or antagonists. These interactions modulate the activity of receptors and consequently regulate the neuroprotection processes. Some agonists of adenosine receptors increase the epileptic episodes reaction to those compounds. Anti-episode action of adenosine and guanosine as well as agonists and antagonists of nucleoside receptors indicate the possibility of applying the knowledge about these processes towards production of new anti-epileptic medication. Successful anti-epileptic medication may be based on compounds that have the ability to increase the concentration of ectoadenosine i.e. adenosine deaminase inhibitors, adenosine kinase inhibitors or compounds with ability to suppress reverse uptake of nucleosides. Another method to increase the concentration of extracellular adenosine is to increase the activity of 5’-nucleotidase. That in effect will increase the amount of ectoadenosine by degradation of ecto-AMP. There are very promising results revealed that oral administration of guanosine and GMP as well as guanosine by itself given intraperitoneally and intraventricularly what halted epileptic seizures caused by quinolinic acid which is a glutamate agonist.
    Napady drgawkowe są wynikiem uwalniania neurotransmiterów pobudzających do przestrzeni pozakomórkowej. W ośrodkowym układzie nerwowym ektoadenozyna hamuje egzocytozę glutaminianu i asparaginianu, natomiast ektoguanozyna, zwiększając wychwyt zwrotny glutaminianu przez astrocyty, obniża jego stężenie poza komórką. W ten sposób oba nukleozydy uczestniczą w hamowaniu napadu drgawkowego. Nukleozydy te, obniżając stężenie powyższych neurotransmiterów poza komórką, chronią neurony przed śmiercią, pełnią więc funkcję neuroprotekcyjną. W różnych modelach zwierzęcych padaczek eksperymentalnych wykazano, że w przerwaniu napadu drgawkowego uczestniczą receptory adenozynowe A1 i A2A. Ma miejsce współdziałanie leków przeciwpadaczkowych i receptorów adenozynowych, bowiem niektóre z nich, takie jak karbamazepina, działają za pośrednictwem receptorów adenozynowych A1, a niektórzy agoniści receptorów A1 potęgują działanie przeciwdrgawkowe tych leków. Przeciwdrgawkowe działanie adenozyny i guanozyny oraz agonistów i antagonistów receptorów nukleozydowych wskazuje na możliwość wykorzystania wiedzy o tych procesach w projektowaniu nowych leków przeciwpadaczkowych. Skutecznymi lekami przeciwdrgawkowymi mogą okazać się związki zwiększające stężenie ektoadenozyny, takie jak: inhibitory deaminazy adenozyny, kinazy adenozynowej oraz związki hamujące wychwyt zwrotny nukleozydów. Innym sposobem zwiększenia stężenia pozakomórkowej adenozyny jest wzrost aktywności 5’-nukleotydazy powiększającej pulę ektoadenozyny przez degradację ektoAMP. Obiecujące są również rezultaty doustnego podania guanozyny i GMP, a także samej guanozyny podanej dokomorowo i dootrzewnowo, które powodowało przerywanie drgawek wywoływanych przez agonistę glutaminianu – kwas chinolinowy.
    Źródło:
    Aktualności Neurologiczne; 2006, 6, 2; 124-130
    1641-9227
    2451-0696
    Pojawia się w:
    Aktualności Neurologiczne
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    A biochemical study on the level of lipids and glycoproteins in the serum and platelets of liver cirrhotic bleeders
    Autorzy:
    Vijayalakshmi, Sivagurunathan
    Geetha, Arumugam
    Jeyachristy, Sam
    Powiązania:
    https://bibliotekanauki.pl/articles/1041294.pdf
    Data publikacji:
    2006
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    glycoproteins
    lipids
    cirrhosis
    adenosine triphosphatases
    platelets
    Opis:
    Bleeding complication and abnormal platelet functions are associated with liver cirrhosis. The aim of the present investigation was to assess the functional integrity of platelets in terms of lipids like cholesterol and phospholipids, glycoproteins and membrane-bound enzymes. Liver cirrhotic patients with bleeding complications were studied. Age and sex matched normal healthy volunteers were also involved in this study as a control group. Levels of cholesterol, phospholipids, glycoproteins and adenosine triphosphatases were assessed in isolated platelet membrane fraction. The level of glycoproteins and the activity of adenosine triphosphatases were found to be decreased significantly in cirrhotic patients. The cholesterol/phospholipid ratio was found to be altered significantly, indicating an alteration in the fluidity of platelet membrane. The results of this study reveal that the functional impairment of platelets in liver cirrhotic patients which is responsible for their bleeding tendency might also be due to altered lipid and enzyme levels in platelet membrane.
    Źródło:
    Acta Biochimica Polonica; 2006, 53, 1; 213-220
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Caffeine and neurodegenerative disorders
    Autorzy:
    BOGATKO, Karolina
    SZOPA, Aleksandra
    POLESZAK, Ewa
    Powiązania:
    https://bibliotekanauki.pl/articles/1033776.pdf
    Data publikacji:
    2014
    Wydawca:
    Zakład Opieki Zdrowotnej Ośrodek Umea Shinoda-Kuracejo
    Tematy:
    Alzheimer’s disease
    Parkinson’s disease
    adenosine
    caffeine
    Opis:
    Neurodegenerative disorders are amongst the most dangerous diseases in modern society. At the end of 1990, adenosine receptor antagonists were used to block the adenosine A1 and A2A receptors causing less physical, cellular and molecular damage caused by Alzheimer’s and Parkinson’s diseases. In recent years, an increase in death rate caused by these diseases has been observed among people under 74. Caffeine, as NMDA receptor antagonist, prevents an uncontrolled influx of calcium ions into the interior of the cells exerting a neuroprotective effect and beneficial procognitive effects. There is much evidence that caffeine intake is associated with a reduced risk of Alzheimer’s and Parkinson’s diseases.
    Źródło:
    Medicina Internacia Revuo; 2014, 26, 103; 89-93
    0465-5435
    Pojawia się w:
    Medicina Internacia Revuo
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Expression level of adenosine kinase in rat tissues. Lack of phosphate effect on the enzyme activity.
    Autorzy:
    Sakowicz, Monika
    Grdeń, Marzena
    Pawełczyk, Tadeusz
    Powiązania:
    https://bibliotekanauki.pl/articles/1044105.pdf
    Data publikacji:
    2001
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    rat
    recombinant protein
    phosphate
    tissue distribution
    adenosine kinase
    Opis:
    In this report we describe cloning and expression of rat adenosine kinase (AK) in Esccherichaia coli cells as a fusion protein with 6xHis. The recombinant protein was purified and polyclonal antibodies to AK were generated in rabbits. Immunoblot analysis of extracts obtained from various rat tissues revealed two protein bands reactive with anti-AK IgG. The apparent molecular mass of these bands was 48 and 38 kDa in rat kidney, liver, spleen, brain, and lung. In heart and muscle the proteins that react with AK antibodies have the molecular masses of 48 and 40.5 kDa. In order to assess the relative AK mRNA level in rat tissues we used the multiplex PCR technique with β-actin mRNA as a reference. We found the highest level of AK mRNA in the liver, which decreased in the order kidney >spleen >lung >heart >brain >muscle. Measurement of AK activity in cytosolic fractions of rat tissues showed the highest activity in the liver (0.58 U/g), which decreased in the order kidney >spleen >lung >brain >heart >skeletal muscle. Kinetic studies on recombinant AK as well as on AK in the cytosolic fraction of various rat tissues showed that this enzyme is not affected by phosphate ions. The data presented indicate that in the rat tissues investigated at least two isoforms of adenosine kinase are expressed, and that the expression of the AK gene appears to have some degree of tissue specificity.
    Źródło:
    Acta Biochimica Polonica; 2001, 48, 3; 745-754
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Purinergic signaling in the pancreas and the therapeutic potential of ecto-nucleotidases in diabetes
    Autorzy:
    Cieślak, Marek
    Roszek, Katarzyna
    Powiązania:
    https://bibliotekanauki.pl/articles/1039191.pdf
    Data publikacji:
    2014
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    ATP
    adenosine
    pancreas
    diabetes mellitus
    P-type receptors
    ecto-nucleotidases
    Opis:
    It is widely accepted that purinergic signaling is involved in the regulation of functions of all known tissues and organs. Extracellular purines activate two classes of receptors, P1-adenosine receptors and P2-nucleotide receptors, in a concentration-dependent manner. Ecto-enzymes metabolizing nucleotides outside the cell are involved in the termination of the nucleotide signaling pathway through the release of ligands from their receptors. The pancreas is a central organ in nutrient and energy homeostasis with endocrine, exocrine and immunoreactive functions. The disturbances in cellular metabolism in diabetes mellitus lead also to changes in concentrations of intra- and extracellular nucleotides. Purinergic receptors P1 and P2 are present on the pancreatic islet cells as well as on hepatocytes, adipocytes, pancreatic blood vessels and nerves. The ATP-dependent P2X receptor activation on pancreatic β-cells results in a positive autocrine signal and subsequent insulin secretion. Ecto-NTPDases play the key role in regulation of extracellular ATP concentration. These enzymes, in cooperation with 5'-nucleotidase can significantly increase ecto-adenosine concentration. It has been demonstrated that adenosine, through activation of P1 receptors present on adipocytes and pancreatic islets cells, inhibits the release of insulin. Even though we know for 50 years about the regulatory role of nucleotides in the secretion of insulin, an integrated understanding of the involvement of purinergic signaling in pancreas function is still required. This comprehensive review presents our current knowledge about purinergic signaling in physiology and pathology of the pancreas as well as its potential therapeutic relevance in diabetes.
    Źródło:
    Acta Biochimica Polonica; 2014, 61, 4; 655-662
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Recent advances in studies on biochemical and structural properties of equilibrative and concentrative nucleoside transporters
    Autorzy:
    Podgorska, Marzena
    Kocbuch, Katarzyna
    Pawelczyk, Tadeusz
    Powiązania:
    https://bibliotekanauki.pl/articles/1041310.pdf
    Data publikacji:
    2005
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    nucleoside transporters
    structure
    gene locus
    substrate specificity
    tissue distribution
    adenosine
    Opis:
    Nucleoside transporters (NT) facilitate the movement of nucleosides and nucleobases across cell membranes. NT-mediated transport is vital for the synthesis of nucleic acids in cells that lack de novo purine synthesis. Some nucleosides display biological activity and act as signalling molecules. For example, adenosine exerts a potent action on many physiological processes including vasodilatation, hormone and neurotransmitter release, platelet aggregation, and lipolysis. Therefore, carrier-mediated transport of this nucleoside plays an important role in modulating cell function, because the efficiency of the transport processes determines adenosine availability to its receptors or to metabolizing enzymes. Nucleoside transporters are also key elements in anticancer and antiviral therapy with the use of nucleoside analogues. Mammalian cells possess two major nucleoside transporter families: equilibrative (ENT) and concentrative (CNT) Na+-dependent ones. This review characterizes gene loci, substrate specificity, tissue distribution, membrane topology and structure of ENT and CNT proteins. Regulation of nucleoside transporters by various factors is also presented.
    Źródło:
    Acta Biochimica Polonica; 2005, 52, 4; 749-758
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    The Role of Adenosine A2a Receptors in Antidepressant Activity in An Experimental Animal Model of Depression
    Autorzy:
    DZIUBINA, Anna
    ZYGMUNT, Małgorzata
    FILIPEK, Barbara
    SAŁAT, Kinga
    BRYŁA, Adrian
    LIBROWSKI, Tadeusz
    GDULA-ARGASIŃSKA, Joanna
    Powiązania:
    https://bibliotekanauki.pl/articles/1033607.pdf
    Data publikacji:
    2017
    Wydawca:
    Zakład Opieki Zdrowotnej Ośrodek Umea Shinoda-Kuracejo
    Tematy:
    A2A receptor
    Parkinson’s disease
    adenosine
    antidepressant activity
    depression
    Opis:
    Intensive studies on the role of adenosine A2A receptors in Parkinson’s disease have been carried out for many years,. These studies have indicated that the antagonists of these receptors not only alleviate motor deficits but also exhibit neuroprotective effects in various animal models. Little is known about the role of these receptors in ailments accompanying Parkinson’s disease, such as depression and anxiety. This paper provides a summary of existing research on the role of A2A receptors in comorbid depression in Parkinson’s disease.
    Źródło:
    Medicina Internacia Revuo; 2017, 28, 109; 228-236
    0465-5435
    Pojawia się w:
    Medicina Internacia Revuo
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Elevation of the adenylate pool in rat cardiomyocytes by S-adenosyl-L-methionine.
    Autorzy:
    Smolenski, Ryszard
    Powiązania:
    https://bibliotekanauki.pl/articles/1044271.pdf
    Data publikacji:
    2000
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    cardiomyocytes
    ribose
    adenylate pool
    ATP
    adenine
    S-adenosyl-L-methionine
    adenosine
    Opis:
    Rapid resynthesis of the adenylate pool in cardiac myocytes is important for recovery of contractility and normal function of regulatory mechanisms in the heart. Adenosine and adenine are thought to be the most effective substrates for nucleotide synthesis, but the possibility of using other compounds has been studied very little in cardiomyocytes. In the present study, the effect of S-adenosyl-L-methionine (SAM) on the adenylate pool of isolated cardiomyocytes was investigated and compared to the effect of adenine and adenosine. Adult rat cardiomyocytes were isolated using the collagenase perfusion technique. The cells were incubated in the presence of adenine derivatives for 90 min followed by nucleotide determination by HPLC. The concentrations of adenine nucleotides expressed in nmol/mg of cell protein were initially 22.1 ± 1.4, 4.0 ± 0.3 and 0.70 ± 0.08 for ATP, ADP and AMP, respectively (n = 10, ±S.E.M.), and the total adenylate pool was 26.8 ± 1.6. In the presence of 1.25 mM SAM in the medium, the adenylate pool increased by 5.2 ± 0.4 nmol/mg of cell protein, but only if 1 mM ribose was additionally present in the medium. No changes were observed with SAM alone. A similar increase (by 4.9 ± 0.6 nmol/mg protein) was observed after incubation with 1.25 mM adenine plus 1 mM ribose, but no increase was observed if ribose was omitted. Adenosine at 0.1 or 1.25 mM concentrations also caused an increase in the adenylate pool (by 5.2 ± 1.0 and 5.2 ± 0.9 nmol/mg protein, respectively), which in contrast to the SAM or adenine was independent of the additional presence of ribose. Thus, S-adenosyl-L-methionine could be used as a precursor of the adenylate pool in cardiomyocytes, which is as efficient in increasing the adenylate pool after 90 min of incubation as adenosine or adenine. Nucleotide synthesis from SAM involves the formation of adenine as an intermediate with its subsequent incorporation by adenine phosphoribosyltransferase.
    Źródło:
    Acta Biochimica Polonica; 2000, 47, 4; 1171-1178
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Low-active high-density Noctiluca scintillans cells in surface seawater
    Autorzy:
    Tada, K.
    Asahi, T.
    Kitatsuji, S.
    Nomura, M.
    Yamaguchi, H.
    Ichimi, K.
    Powiązania:
    https://bibliotekanauki.pl/articles/2079194.pdf
    Data publikacji:
    2020
    Wydawca:
    Polska Akademia Nauk. Instytut Oceanologii PAN
    Tematy:
    Noctiluca scintillans
    cell
    dinoflagellate
    mesozooplankton
    adenosine triphosphate
    sea surface
    surface water
    activity
    Opis:
    Noctiluca scintillans is an important member of the mesozooplankton in terms of biomass and production in the Seto Inland Sea, Japan. The densities and adenosine triphosphate (ATP) contents of N. scintillans cells were measured. Vertical profiles of N. scintillans cellular activity in the coastal water were determined and the ATP contents were high at middle layers, with a maximum depth of 10 m. ATP contents were low in the surface and lower layers. These results suggest that active N. scintillans cells in subsurface layers with low density play an important role in the coastal ecosystem, and high-density cells in the surface water are not active.
    Źródło:
    Oceanologia; 2020, 62, 3; 402-407
    0078-3234
    Pojawia się w:
    Oceanologia
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Plant nucleoside 5-phosphoramidate hydrolase; simple purification from yellow lupin (Lupinus luteus) seeds and properties of homogeneous enzyme
    Autorzy:
    Guranowski, Andrzej
    Wojdyła, Anna
    Rydzik, Anna
    Stepiński, Janusz
    Jemielity, Jacek
    Powiązania:
    https://bibliotekanauki.pl/articles/1039966.pdf
    Data publikacji:
    2011
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    yellow lupin
    Lupinus luteus
    adenosine 5'-phosphoramidate
    purification to homogeneity
    nucleoside 5'-phosphoramidase
    Opis:
    Adenosine 5'-phosphoramidate (NH2-pA) is an uncommon natural nucleotide of poorly understood biochemistry and function. We studied a plant enzyme potentially involved in the catabolism of NH2-pA. A fast and simple method comprising extraction of yellow lupin (Lupinus luteus) seed-meal with a low ionic strength buffer, ammonium sulfate and acetone fractionations, removal of contaminating proteins by heat denaturation, and affinity chromatography on AMP-agarose, yielded homogenous nucleoside 5'-phosphoramidase. Mass spectrometric analysis showed that the lupin hydrolase exhibits closest similarity to Arabidopsis thaliana Hint1 protein. The substrate specificity of the lupin enzyme, in particular its ability to split the P-S bond in adenosine 5'-phosphorothioate, is typical of known Hint1 proteins. Adenosine 5'-phosphofluoride and various derivatives of guanosine 5'-phosphoramidate were also substrates. Neither common divalent metal cations nor 10 mM EDTA or EGTA affected the hydrolysis of NH2-pA. The enzyme functions as a homodimer (2 × 15 800 Da). At the optimum pH of 7.0, the Km for NH2-pA was 0.5 µM and kcat 0.8 s-1 (per monomer active site). The properties of the lupin nucleoside 5'-phosphoramidase are compared with those of its counterparts from other organisms.
    Źródło:
    Acta Biochimica Polonica; 2011, 58, 1; 131-136
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Mucosal adenosine deaminase activity and stump ulcer healing
    Autorzy:
    Namiot, Z
    Namiot, A.
    Stasiewicz, J.
    Marcinkiewicz, M.
    Jaroszewicz, W.
    Gorski, J.
    Powiązania:
    https://bibliotekanauki.pl/articles/69936.pdf
    Data publikacji:
    1995
    Wydawca:
    Polskie Towarzystwo Fizjologiczne
    Tematy:
    gastric ulcer
    ulcer crater
    stump ulcer
    duodenal ulcer
    gastric mucosa
    stomach
    adenosine deaminase
    Źródło:
    Journal of Physiology and Pharmacology; 1995, 46, 2
    0867-5910
    Pojawia się w:
    Journal of Physiology and Pharmacology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    QUERCETIN AMELIORATES INSULIN RESISTANCE CONCOMITANT EARLY CARDIOVASCULAR CHANGES IN EXPERIMENTAL RATS
    Autorzy:
    KUSHWAH, AJAY S.
    GUPTA, GHANSHYAM D.
    Powiązania:
    https://bibliotekanauki.pl/articles/895413.pdf
    Data publikacji:
    2018-08-31
    Wydawca:
    Polskie Towarzystwo Farmaceutyczne
    Tematy:
    insulin resistance
    oxidative stress
    myocardium
    quercetin
    high-fructose diet
    5' Adenosine Monophosphate-activated Protein Kinase
    Opis:
    Quercetin is a dietary flavonoid found in a wide range of fruits and vegetables; it has diverse biological activities, possesses beneficial effects in ameliorating diabetic complications, apart from the effect of quercetin on fructose feed induced insulin resistance (IR) linked cardiac dysfunction have not been entirely revealed. This study aspires to explore the effect of quercetin on metabolism, oxidative stress, cardiomyocytes damage and cardiac function in IR state. Wistar rats either sex weighing 220-250 g (n ꞊8), were divided into four groups, kept on either control diet and high fructose diet and supplement with a quercetin as a test drug and metformin as a standard, at the dose of 50 and 200 mg/ kg; p.o., respectively. Daily measured body weight, feed, and water intake for 35 days, Oral glucose tolerance test (OGTT) performed in animals on the 32nd day. At end of the study (36th day), measured hemodynamic parameters after that estimation of various biochemical parameters. Finally, the animals were sacrificed for isolation of tissues and measured heart weight, the oxidative stress level of heart and histopathological changes. Treatment of quercetin with fructose-fed ameliorated all the parameters revile by the contrast of IR rats. The outcome of quercetin associated improves insulin sensitivity, normalized lipid profile, abolish hemodynamic changes, oxidative stress and cardiac injury markers within fructose-fed, and lesser histopathological changes were observed contrast with IR rats. These beneficial effects of quercetin mediated by improving insulin sensitivity and metabolism; reduced oxidative stress could potentially be used to ameliorate the myocardial damage.
    Źródło:
    Acta Poloniae Pharmaceutica - Drug Research; 2018, 75, 4; 977-987
    0001-6837
    2353-5288
    Pojawia się w:
    Acta Poloniae Pharmaceutica - Drug Research
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Dissociation of parathyroid hormone and cyclic-3',5'AMP effects on Na-Pi uptake by cells isolated from proximal straight tubules of rat kidney
    Autorzy:
    Rybczynska, A.
    Angielski, S.
    Hoppe, A.
    Powiązania:
    https://bibliotekanauki.pl/articles/69520.pdf
    Data publikacji:
    2000
    Wydawca:
    Polskie Towarzystwo Fizjologiczne
    Tematy:
    proximal tubule
    adenosine 3',5'-cyclic monophosphate
    parathyroid hormone
    kidney
    3-isobutyl-1-methylxanthine
    rat
    Źródło:
    Journal of Physiology and Pharmacology; 2000, 51, 2
    0867-5910
    Pojawia się w:
    Journal of Physiology and Pharmacology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Effect of platelet activating factor (PAF) supplementation in semen extender on viability and ATP content of cryopreserved canine spermatozoa
    Autorzy:
    Kordan, W.
    Lecewicz, M.
    Strzezek, R.
    Dziekonska, A.
    Fraser, L.
    Powiązania:
    https://bibliotekanauki.pl/articles/30804.pdf
    Data publikacji:
    2010
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    platelet-activating factor
    supplementation
    semen
    viability
    spermatozoon
    cryopreservation
    cryopreserved spermatozoon
    dog
    adenosine triphosphate content
    sperm
    Opis:
    The aim of this study was to investigate the effect of platelet activating factor (PAF) on the quality characteristics of cryopreserved canine spermatozoa. Cryopreserved semen of 5 mixed-breed dogs was treated with different concentrations of exogenous PAF (1 × 10⁻³M, 1 × 10⁻⁴M, 1 × 10⁻⁵M and 1 × 10-6M) and examined at different time intervals (0, 30, 60 and 120 min). Cryopreserved semen treated without PAF was used as the control. Sperm quality was evaluated for motility (computer-assisted semen analysis, CASA), mitochondrial function (JC-1/PI assay) and plasma membrane integrity (SYBR-14/PI assay and Hoechst 33258). Also, ATP content of spermatozoa was determined using a bioluminescence assay. Treatment of cryopreserved semen with 1 × 10⁻³ M PAF at 120 min of incubation resulted in significantly higher total sperm motility compared with the control. It was observed that PAF-improved total sperm motility was concurrent with enhanced sperm motility patterns after treatment of cryopreserved semen. Treatment of cryopreserved semen with PAF did not improve either sperm mitochondrial function or plasma membrane integrity, as monitored by different fluorescent membrane markers. Furthermore, ATP content of cryopreserved spermatozoa was significantly higher when PAF was used at a concentration of 1 × 10⁻³ M compared with the control and other PAF treatments, regardless of the incubation time. The findings of this study indicated that treatment with 1 × 10⁻³ M PAF at 120 min of incubation rendered better quality of cryopreserved canine semen, which was associated with improved sperm motility parameters and ATP content. It can be suggested that exogenous PAF addition is beneficial as a supplement for canine semen extender used for cryopreservation.
    Źródło:
    Polish Journal of Veterinary Sciences; 2010, 13, 4
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Adenosine triphosphate in the marine boundary layer in the Southern Baltic Sea
    Autorzy:
    Pryputniewicz, D.
    Falkowska, L.
    Burska, D.
    Powiązania:
    https://bibliotekanauki.pl/articles/48770.pdf
    Data publikacji:
    2002
    Wydawca:
    Polska Akademia Nauk. Instytut Oceanologii PAN
    Tematy:
    organic matter
    marine boundary
    adenosine triphosphate
    coastal water
    vertical distribution
    spring
    microlayer
    Baltic Sea
    marine ecosystem
    Opis:
    Changes in adenosine triphosphate (ATP) concentration were measured in the offshore and coastal waters of the GdańskBasi n in spring. As regards the vertical distribution, it was found that high ATP concentrations occurred mainly in the euphotic layer (above the thermocline) and near the bottom (below the halocline). The high concentrations of ATP in the euphotic layer resulted from primary and secondary production, while the other maximum was due to the presence of bacteria actively degrading organic matter. Changes in ATP concentration in the euphotic layer were closely correlated with the phase of the day. An increase in ATP concentrations in the surface microlayer was observed in the evening and at night, probably as a result of heterotroph proliferation. During daylight, ATP production was inhibited by increasing radiation, hence its concentrations in the sea surface microlayer were considerably lower. Strong winds exerted a significant influence on ATP concentrations in the surface microlayer and in the subsurface water. Windstress depressed ATP concentrations. The biomass of living microorganisms in the microlayer was comparable with the microbiomass beneath the halocline.
    Źródło:
    Oceanologia; 2002, 44, 4
    0078-3234
    Pojawia się w:
    Oceanologia
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Cross-talk between the ATP and ADP nucleotide receptor signalling pathways in glioma C6 cells.
    Autorzy:
    Czajkowski, Rafał
    Barańska, Jolanta
    Powiązania:
    https://bibliotekanauki.pl/articles/1043690.pdf
    Data publikacji:
    2002
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    ATP
    cross-talk
    glioma C6 cells
    ADP
    phospholipase C
    nucleotide receptors
    adenylyl cyclase
    signalling pathways
    adenosine
    Opis:
    In this review we summarize the present status of our knowledge on the enzymes involved in the extracellular metabolism of nucleotides and the receptors involved in nucleotide signalling. We focus on the mechanism of the ATP and ADP signalling pathways in glioma C6, representative of the type of nonexcitable cells. In these cells, ATP acts on the P2Y2 receptor coupled to phospholipase C, whereas ADP on two distinct P2Y receptors: P2Y1 and P2Y12. The former is linked to phospholipase C and the latter is negatively coupled to adenylyl cyclase. The possible cross-talk between the ATP-, ADP- and adenosine-induced pathways, leading to simultaneous regulation of inositol 1,4,5-trisphosphate and cAMP mediated signalling, is discussed.
    Źródło:
    Acta Biochimica Polonica; 2002, 49, 4; 877-889
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Influence of dipeptidyl peptidase IV on enzymatic properties of adenosine deaminase
    Autorzy:
    Sharoyan, Svetlana
    Antonyan, Alvard
    Mardanyan, Sona
    Lupidi, Giulio
    Cristalli, Gloria
    Powiązania:
    https://bibliotekanauki.pl/articles/1041210.pdf
    Data publikacji:
    2006
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    protein-protein interaction
    large and small adenosine deaminases
    CD26-dipeptidyl peptidase IV
    enzyme-substrate and enzyme-inhibitor interactions
    Opis:
    The importance of ADA (adenosine deaminase) in the immune system and the role of its interaction with an ADA-binding cell membrane protein dipeptidyl peptidase IV (DPPIV), identical to the activated immune cell antigen, CD26, has attracted the interest of researchers for many years. To investigate the specific properties in the structure - function relationship of the ADA/DPPIV-CD26 complex, its soluble form, identical to large ADA (LADA), was isolated from human blood serum, human pleural fluid and bovine kidney cortex. The kinetic constants (Km and Vmax) of LADA and of small ADA (SADA), purified from bovine lung and spleen, were compared using adenosine (Ado) and 2'-deoxyadenosine (2'-dAdo) as substrates. The Michaelis constant, Km, evidences a higher affinity of both substrates (in particular of more toxic 2'-dAdo) for LADA and proves the modulation of toxic nucleoside neutralization in the extracellular medium due to complex formation between ADA and DPPIV-CD26. The values of Vmax are significantly higher for SADA, but the efficiency, Vmax /Km, in LADA-catalyzed 2'-dAdo deamination is higher than that in Ado deamination. The interaction of all enzyme preparations with derivatives of adenosine and erythro-9-(2-hydroxy-3-nonyl)adenine (EHNA) was studied. 1-DeazaEHNA and 3-deazaEHNA demonstrate stronger inhibiting activity towards LADA, the DPPIV-CD26-bound form of ADA. The observed differences between the properties of the two ADA isoforms may be considered as a consequence of SADA binding with DPPIV-CD26. Both SADA and LADA indicated a similar pH-profile of adenosine deamination reaction with the optimum at pHs 6.5 - 7.5, while the pH-profile of dipeptidyl peptidase activity of the ADA/DPPIV-CD26 complex appeared in a more alkaline region.
    Źródło:
    Acta Biochimica Polonica; 2006, 53, 3; 539-546
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Jakość wody pitnej po zastosowaniu trzystopniowej baterii filtrów i dezynfekcji UVC
    The quality of drinking water after application of a three-stage home water filter and UVC radiation
    Autorzy:
    Rożej, A.
    Powiązania:
    https://bibliotekanauki.pl/articles/960234.pdf
    Data publikacji:
    2015
    Wydawca:
    Towarzystwo Chemii i Inżynierii Ekologicznej
    Tematy:
    woda pitna
    dezynfekcja
    bakterie heterotroficzne
    filtry domowe do wody
    adenozynotrifosforan
    ATP
    stagnacja wody
    promieniowanie UVC
    drinking water
    disinfection
    heterotrophic plate count
    HPC
    home water filter
    adenosine tri-phosphate
    stagnation
    UVC radiation
    Opis:
    Celem pracy było zbadanie zmian jakości wody pitnej po jej dodatkowym uzdatnieniu za pomocą baterii filtrów domowych: z polipropylenu (PP), węgla aktywnego i żywicy jonowymiennej oraz dezynfekcji z użyciem lampy UV-C. Doświadczenie prowadzono w budynku użyteczności publicznej przez 70 dni. Po nocnej stagnacji cztery kolejne próbki wody, każda o objętości 500 cm3, pobierano bezpośrednio po otworzeniu zaworu czerpalnego oraz po spuszczeniu 10 litrów wody wodociągowej, jak również dodatkowo uzdatnionej. W próbkach wody oznaczano temperaturę, skład chemiczny, stężenie chloru wolnego i całkowitego, liczbę bakterii heterotroficznych oraz stężenie wolnego i wewnątrzkomórkowego adenozynotrifosforanu (ATP). Jakość mikrobiologiczna wody po dodatkowym uzdatnieniu i dezynfekcji za pomocą lampy UV-C poprawiła się tylko w pierwszych dniach trwania doświadczenia. W czasie trwania eksperymentu doszło do znacznego wzrostu liczebności bakterii heterotroficznych i stężenia ATP w wodzie filtrowanej, na co decydujący wpływ miały prawdopodobnie: niskie stężenie czynnika dezynfekującego oraz wysoka temperatura wody (ponad 40ºC) podgrzewanej podczas zastoju w zestawie filtrów w wyniku pracy ciągłej lampy UV.
    The influence of three-stage home water filter consisting of polypropylene (PP), activated carbon and ion exchange filters as well as UV-C lamp on a drinking water quality was studied. Experiment was conducted for 70 days in a public building. After night stagnation the water samples were taken directly from water installation and after additional treatment. In both treatments the four consecutive samples of 500 cm3 volume (0.5; 1; 1.5; 2) were sampled immediately after opening the flow and the one sample after 10 dm3 of flushing. Temperature, concentration of free and total chlorine, some inorganic components, free and total adenosine tri-phosphate (ATP) as well as heterotrophic plate count (HPC) were measured immediately. Microbial quality of drinking water treated by battery filters and UV-C radiation has been improved only in first days of the system operation. With time the lack of disinfecting agent, the high temperature exceeding 40ºC as the result of continuous operation mode of UV-C lamp and the water stagnation affected the high increase in the HPC and ATP concentration inside a basin mixer.
    Źródło:
    Proceedings of ECOpole; 2015, 9, 2; 721-728
    1898-617X
    2084-4557
    Pojawia się w:
    Proceedings of ECOpole
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
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