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    Wyświetlanie 1-23 z 23
    Tytuł:
    Localization of the attachment site of oligoglucans to Mesorhizobium loti HAMBI 1148 murein
    Autorzy:
    Karaś, Magdalena
    Russa, Ryszard
    Powiązania:
    https://bibliotekanauki.pl/articles/1040650.pdf
    Data publikacji:
    2009
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    oligoglucan
    murein
    MALDI-TOF
    deamination
    Mesorhizobium
    Opis:
    The location and nature of the linkage between peptidoglycan and oligoglucans in the cell wall of Mesorhizobium loti HAMBI 1148 have been defined by the analysis of nitrous acid deamination of peptidoglycan glucosaminyl residues. The MurNH2-Glcn fraction was obtained after converting deaminoacylated and N-deacetylated muramyl residues in the cell wall preparation to lactam forms which were stable during subsequent deamination, followed by reduction and opening of the lactams. GC/MS analysis of this material, subjected to partial hydrolysis and reduction or to methanolysis followed by peracetylation, confirmed the presence of glucosyl residues glycosidically attached to muramic acid. The MALDI-TOF spectroscopic analysis of the deaminated material also revealed the presence of [M-H]- or [M+Na-2H]- ions representing fragments containing muramic acid with one to three linked glucose residues. The analysis of fully methylated neutral oligosaccharides released from the peptidoglycan with lysozyme followed by borohydride reduction showed the presence of di- and trisaccharides lacking the reducing end.
    Źródło:
    Acta Biochimica Polonica; 2009, 56, 1; 155-160
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Nasal carriage of various staphylococcal species in small ruminant lentivirus-infected asymptomatic goats
    Autorzy:
    Moroz, A.
    Szaluś-Jordanow, O.
    Czopowicz, M.
    Brodzik, K.
    Petroniec, V.
    Augustynowicz-Kopeć, E.
    Lutyńska, A.
    Roszczynko, M.
    Gołoś-Wójcicka, A.
    Korzeniowska-Kowal, A.
    Gamian, A.
    Mickiewicz, M.
    Frymus, T.
    Petelicka, H.
    Kaba, J.
    Powiązania:
    https://bibliotekanauki.pl/articles/2087322.pdf
    Data publikacji:
    2020
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    caprine arthritis-encephalitis
    MALDI-TOF
    PFGE
    Staphylococcus aureus
    Źródło:
    Polish Journal of Veterinary Sciences; 2020, 23, 2; 203-209
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Identification of bacterial species in milk by MALDI-TOF and assessment of some oxidant-antioxidant parameters in blood and milk from cows with different health status of the udder
    Autorzy:
    Ozbey, G.
    Cambay, Z.
    Yilmaz, S.
    Aytekin, O.
    Zigo, F.
    Ozçelik, M.
    Otlu, B.
    Powiązania:
    https://bibliotekanauki.pl/articles/16539172.pdf
    Data publikacji:
    2022
    Wydawca:
    Polska Akademia Nauk. Czasopisma i Monografie PAN
    Tematy:
    dairy cows
    CMT
    mastitis
    malondialdehyde
    glutathione
    MALDI-TOF
    Opis:
    This study aimed to identify bacterial pathogens in milk samples from dairy cows with subclinical and clinical mastitis as well as to assess the concentrations of oxidant-antioxidant parameters [malondialdehyde (MDA), reduced glutathione (GSH), and total GSH levels] in both blood and milk samples. From a total of 200 dairy cows in 8 farms, 800 quarter milk samples obtained from each udder were tested in the laboratory for the presence of udder pathogens. Cultivated bacteria causing intramammary infection from milk samples were identified by Matrix-Assisted Laser Desorption/Ionization-Time of Flight (MALDI-TOF). In addition, from tested animals 60 cows were selected includıng 20 healthy cows that were CMT negative, 20 cows with subclinical mastitis (SM), and 20 cows with clinical mastitis (CM) for detection of MDA, GSH, and total GSH levels in blood and milk samples. Three hundred and eighty (47.5%; 380/800), 300 (37.5%; 300/800), and 120 (15%; 120/800) of milk samples, respectively were CMT positive or SM and CM, and those positives were cows from different farms. We observed that 87.4% (332/380), 25.3% (76/300), and 34.2% (41/120) of cows with CMT positive, CMT negative, and CM had bacterial growth. The most predominantly identified bacteria were Staphylococcus chromogenes (18.7%) obtained mainly from SM and Staphylococcus aureus (16.7%) as the most frequent cause of CM. According to our results, dairy cows with CM had the highest MDA levels, the lowest GSH, and total GSH levels in both blood and milk samples however, high MDA levels and low GSH levels in milk samples with SM were observed. Based on our results, lipid oxidant MDA and antioxidant GSH could be excellent biomarkers of cow’s milk for developing inflammation of the mammary gland. In addition, there was no link between nutrition and MDA and GSH levels.
    Źródło:
    Polish Journal of Veterinary Sciences; 2022, 25, 2; 269-277
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Mass spectrometric analysis of head-to-tail connected cyclic peptides.
    Autorzy:
    Macht, Marcus
    Pelzing, Matthias
    Palloch, Petra
    Sauerland, Volker
    Volz, Jürgen
    Powiązania:
    https://bibliotekanauki.pl/articles/1044055.pdf
    Data publikacji:
    2001
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    electrospray
    post source decay
    MALDI
    mass spectrometry
    peptide sequencing
    Opis:
    Tandem mass spectrometry is an extremly useful tool for high sensitive sequence identification of peptides. In the case of cyclic peptides fragmentation can easily be performed for sequence analysis. However, analysis is usually tedious due to the lack of a defined beginning and end of the sequence. Since cyclic peptides are a highly interesting class of compounds especially for the pharmaceutical industry, ways have to be found to identify their structures. In this work we demonstrate how software and dedicated analytical strategies can be used for detailed analysis of these substances.
    Źródło:
    Acta Biochimica Polonica; 2001, 48, 4; 1109-1112
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Characterisation of Mesorhizobium huakuii cyclic β-glucan.
    Autorzy:
    Choma, Adam
    Komaniecka, Iwona
    Powiązania:
    https://bibliotekanauki.pl/articles/1043423.pdf
    Data publikacji:
    2003
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    OPG
    NMR
    cyclic β-glucan
    Mesorhizobium huakuii
    MALDI-TOF
    osmoregulation
    Opis:
    Periplasmic and extracellular glucans of Mesorhizobium huakuii were isolated and characterized by compositional and MALDI-TOF analyses, as well as 1H and 13C NMR spectroscopy. It was shown that M. huakuii produces a cyclic β-glucan composed entirely of nonbranched glucose chains and unmodified by nonsugar substituents. The degree of polymerisation of the cyclic oligosaccharides was estimated to be in the range from 17 to 28. The most abundant glucan molecules contained 22 glucose residues. Glucose residues within the glucan were connected by β-(1,2) glycosidic linkages. The cyclic glucan produced by M. huakuii is quite similar to the periplasmic β-(1,2) glucans synthesized by Agrobacterium and Sinorhizobium genera. The synthesis of β-glucan in M. huakuii is osmoregulated and this glucan could function as an osmoprotectant in free living cells.
    Źródło:
    Acta Biochimica Polonica; 2003, 50, 4; 1273-1281
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    The structure of the oligosaccharides of α3β1 integrin from human ureter epithelium (HCV29) cell line.
    Autorzy:
    Lityńska, Anna
    Pocheć, Ewa
    Hoja-Łukowicz, Dorota
    Kremser, Elżbieta
    Laidler, Piotr
    Amoresano, Angela
    Monti, Chiara
    Powiązania:
    https://bibliotekanauki.pl/articles/1043787.pdf
    Data publikacji:
    2002
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    glycosylation
    α3β1 integrin
    cell line
    MALDI MS
    Opis:
    There is a growing line of evidence that glycosylation of α and β subunits is important for the function of integrins. Integrin α3β1, from human ureter epithelium cell - line HCV29, was isolated by affinity chromatography on laminin GD6 peptide. Characterization of its carbohydrate moieties was carried out using sodium dodecyl sulfate/polyacrylamide gel electrophoresis followed by Western blotting on Immobilon P and on-blot deglycosylation with peptide N-glycosidase-F. Profiles of N-glycans for each subunit were obtained by matrix-assisted laser desorption/ionization mass spectrometry. Our findings demonstrated, in both subunits of integrin α3β1, the presence of complex type oligosaccharides with a wide heterogeneity. Bi- tri- and tetraantennary structures were the most common, while high-mannose type structures were minor. Also the presence of short poly-N-acetyllactosamine entities was shown. These results show that while the predominant oligosaccharides of both subunits are identical, some slight differences between them do exist.
    Źródło:
    Acta Biochimica Polonica; 2002, 49, 2; 491-500
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Construction and verification of mathematical model of mass spectrometry data
    Konstrukcja i weryfikacja matematycznego modelu danych widm masowych
    Autorzy:
    Plechawska-Wójcik, M.
    Powiązania:
    https://bibliotekanauki.pl/articles/408752.pdf
    Data publikacji:
    2013
    Wydawca:
    Politechnika Lubelska. Wydawnictwo Politechniki Lubelskiej
    Tematy:
    Maldi-Tof mass spectrometry
    Gaussians
    Gaussian mixture models
    SVM-RFE classification
    spektrometria masowa Maldi-Tof
    rozkłady Gaussa
    mieszaniny rozkładów Gaussa
    klasyfikacja SVM-RFE
    Opis:
    The article presents issues concerning construction, adjustment and implementation of mass spectrometry mathematical model based on Gaussians and Mixture Models and the mean spectrum. This task is essential to the analysis and it needs specification of many parameters of the model.
    Artykuł przedstawia kwestie związane z konstrukcją, dopasowaniem i implementacją modelu matematycznego widm masowych opartego o rozkłady normalne i mieszaniny rozkładów oraz o widmo średnie. To zadanie jest kluczowe dla analizy, wymaga też określenia wielu parametrów modelu.
    Źródło:
    Informatyka, Automatyka, Pomiary w Gospodarce i Ochronie Środowiska; 2013, 1; 9-14
    2083-0157
    2391-6761
    Pojawia się w:
    Informatyka, Automatyka, Pomiary w Gospodarce i Ochronie Środowiska
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Probiotic Bacillus sp. environmental strains as a component of improved dishwasher cleaning product
    Autorzy:
    Banaszczyk, Justyna
    Jędrzejczak, Gabriela
    Zarzeczańska, Dorota
    Ramotowska, Sandra
    Fiutak, Marta
    Skowron, Małgorzata
    Ossowski, Tadeusz
    Skowron, Piotr
    Jeżewska-Frąckowiak, Joanna
    Powiązania:
    https://bibliotekanauki.pl/articles/1179658.pdf
    Data publikacji:
    2017
    Wydawca:
    Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
    Tematy:
    Dishwasher microbiota
    MALDI-TOF microorganism identification
    Probiotic microorganisms
    Surface colonization
    Opis:
    The use of probiotics has become a mainstream application in pharmaceutical, food, cosmetic and cleaning technologies. Probiotic microorganisms seem to be a remedy for the problem of undesired, persistent microbiological contamination or uncontrolled biofilm formation in the automatic household equipments, dedicated to the undirect contact with food. We propose the mixture of commonly widespread and bio-safe environmental probiotic Bacillus sp. strains as a component of an improved cleaning product for automatic dishwashers. Our design of probiotic-improved cleaning product combines the chemical detergent formula and probiotic Bacillus sp. preparation in two separate pockets of self-soluble cleaning capsule. To pinpoint the validity of an improved probiotic-based cleaning product design, we present a case study of the microflora from the chosen localizations of an old household dishwasher. The swab collecting, followed by bacterial cultivation and MALDI-TOF identification revealed Enterobacter cloacae and Pseudomonas oleovorans forming the major inner microflora of an old household dishwasher, where a commonly available dishwashing products were used. We applied probiotic Bacillus sp. improved cleaning product to a model dishwasher in a conventional cleaning cycle and conducted the before-after trial to identify the inner microflora in chosen localizations. Using standard microbiological Gram-staining method with microscopic observation, followed by MALDI-TOF identification we confirmed, that Bacillus sp. replaced Acinetobacter sp., residing formerly model dishwasher filter and water drain. Conducted experiments have shown the potential for use of the chosen probiotic Bacillus sp. strains mixture as an addition for encapsulated formula of cleaning product, to provide microbiologically safe and controllable conditions during the conventional dishwasher cleaning cycle.
    Źródło:
    World Scientific News; 2017, 72; 141-149
    2392-2192
    Pojawia się w:
    World Scientific News
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Identification of a CAPA-PVK [Ixori-PVK] from single cells of the gulf Gulf Coast tick, Amblyomma maculatum
    Autorzy:
    Neupert, S.
    Russell, W.K.
    Russell, D.H.
    Strey, O.F.
    Teel, P.D.
    Strey, A.
    Nachman, R.J.
    Powiązania:
    https://bibliotekanauki.pl/articles/55404.pdf
    Data publikacji:
    2008
    Wydawca:
    Sieć Badawcza Łukasiewicz - Instytut Przemysłu Organicznego
    Tematy:
    diuresis
    MALDI analysis
    single cell
    periviscerokinin
    Boophilus microplus
    Ixodidae
    immunocytochemistry
    Gulf Coast tick
    amino acid sequence
    MALDI-TOF mass spectrometry
    neurohormone
    Ixodes ricinus
    Amblyomma maculatum
    Opis:
    MALDI-TOF/TOF tandem mass spectrometry has been applied to determine the complete sequence of a CAPA-PVK in the Gulf Coast tick, Amblyomma maculatum. Single cell analysis allowed the identification of the amino acid sequence of Ixori-PVK (PALIPFPRV-NH2), a periviscerokinin which had previously been identified from two other ticks, Ixodes ricinus and Boophilus microplus. The identification indicates greater conservation of sequence for the CAPA-PVK/CAP2b family in ticks as compared with insects. Side-chain fragmentation experiments provided data to distinguish between Leu/Ile ambiguities. The tick CAPA peptide shows a high sequence homology with other members of the insect periviscerokinin/ CAP2b peptides, which are associated with the regulation of critical physiological processes such as diuresis. Thus, the identification of this neuropeptide will provide the experimental basis to better understand regulation of water balance in these arthropods, providing a potential opportunity to develop neuropeptide-based control strategies against these livestock pests.
    Źródło:
    Pestycydy; 2008, 1-2; 67-73
    0208-8703
    Pojawia się w:
    Pestycydy
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Gwieździsty poli(tlenek propylenu) jako materiał czuły na promieniowanie rentgenowskie
    Star-shaped poly(propylene oxide) as an x-ray sensitive material
    Autorzy:
    Swinarew, A. S.
    Łężniak, M.
    Gabor, J.
    Flak, T.
    Kubik, K.
    Okła, H.
    Bielicka, A.
    Kwaśniewska, A.
    Guia, A.
    Swinarew, B.
    Powiązania:
    https://bibliotekanauki.pl/articles/946325.pdf
    Data publikacji:
    2018
    Wydawca:
    Sieć Badawcza Łukasiewicz - Instytut Inżynierii Materiałów Polimerowych i Barwników
    Tematy:
    dozymetria
    poliole
    FTIR
    chromatografia żelowa
    MRI
    MALDI-TOF
    dosimetry
    polyols
    gel chromatography
    Opis:
    Aparatura wykorzystywana w teleradioterapii oraz obrazowaniu, wraz z postępem technologicznym, staje się coraz bardziej precyzyjna, jednak niezależnie od stopnia zaawansowania aparatury pomiarowej, przed napromieniowaniem obszarów nowotworowych pacjenta dokonuje się szeregu symulacji rozkładu dawki promieniowania. Nowe generacje dozymetrów żelowych pozwalają na coraz dokładniejszą ocenę zaabsorbowanych dawek oraz przestrzennego rozkładu promieniowania, co jest bardzo ważnym etapem w radioterapii, gdyż pozwala na efektywniejsze naświetlenie obszarów nowotworowych promieniowaniem jonizującym, minimalizując jednocześnie obszar naświetlania zdrowych tkanek. Celem pracy było otrzymanie poli(tlenku propylenu) jako dozymetru żelowego, jego charakterystyka pod kątem procesów sieciowania oraz degradacji i analiza jakościowa powstałych produktów reakcji chemicznych wywołanych promieniowaniem jonizującym. Do badań wykorzystano gwiaździsty poli(tlenek propylenu) przed oraz po napromieniowaniu. Próbką referencyjną był poli(tlenek propylenu) nie poddany ekspozycji na żaden rodzaj promieniowania w tym promieniowania rentgenowskiego. W celu określenia stopnia usieciowania, degradacji polimeru wykorzystano technikę chromatografii żelowej, na podstawie której określono średnie masy cząsteczkowe. Do jakościowej analizy produktów wytworzonych w skutek napromieniowania wykorzystano technikę FTIR/ATR. Określenie grup funkcyjnych badanego polimeru pozwoliło na ocenę zmian chemicznych jakie wystąpiły poprzez napromieniowanie.
    Equipment used in teleradiotherapy and imaging along with technological progress becomes more and more precise, however, irrespective of the degree of advancement of the measuring apparatus, before the radiation of the tumor areas of the patient, a series of simulations of the radiation dose distribution is performed. New generations of gel dosimeters allow more accurate assessment of dose absorbed, and the spatial distribution of radiation, which is a very important stage in radiotherapy because it allows effective exposure of the tumor areas with ionizing radiation while minimizing the irradiation of healthy tissue area. The aim of the study was to obtain polypropylene oxide as a gel dosimeter and to characterize crosslinking and degradation processes, qualitative analysis of chemical reaction products generated by ionizing radiation. The study used a poly (propylene oxide) before and after irradiation. The reference sample was poly (propylene), not exposed to any type of radiation, including X-rays. To determine the degree of crosslinking, degradation of the polymer in the technique of gel permeation chromatography was used, based on which the average molecular weights were determined. FTIR / ATR technique was used for the qualitative analysis of irradiated products. Determination of the functional groups of the examined polymer allowed the assessment of the chemical changes that had occurred by irradiation.
    Źródło:
    Przetwórstwo Tworzyw; 2018, T. 24, Nr 1 (181), 1 (181); 69-85
    1429-0472
    Pojawia się w:
    Przetwórstwo Tworzyw
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Investigation of asparagine deamidation in a SOD1-based biosynthetic human insulin precursor by MALDI-TOF mass spectrometry
    Autorzy:
    Bierczyńska-Krzysik, Anna
    Łopaciuk, Małgorzata
    Pawlak-Morka, Renata
    Stadnik, Dorota
    Powiązania:
    https://bibliotekanauki.pl/articles/1039302.pdf
    Data publikacji:
    2014
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    asparagine deamidation
    insulin precursor
    superoxide dismutase
    peptide mass fingerprinting
    MALDI-TOF MS
    Opis:
    A biosynthetic human insulin precursor displayed enhanced susceptibility to deamidation at one particular site. The present study was undertaken to monitor progress of precursor deamidation at successive manufacturing stages. MALDI-TOF/TOF MS in combination with controlled endoproteinase Glu-C and endoproteinase Asp-N proteolysis was used for rapid and unambiguous determination of deamidated residue within the investigated structure. Close inspection of isotopic distribution patterns of peptides resulting from enzymatic digestion enabled determination of distinct precursor forms occurring during the production process. Asn, Asp, isoAsp and succinimide derivatives of the amino acid at position 26 were unambiguously identified. These modifications are related to the leader peptide of a precursor encompassing amino acid sequence corresponding to that of superoxide dismutase [Cu-Zn] (SOD1 1, EC=1.15.1.1). Monitoring of precursor deamidation process at successive manufacturing stages revealed that the protein folding stage was sufficient for a prominent replacement of asparagine by aspartic and isoaspartic acid and the deamidated human insulin precursor constituted the main manufactured product. Conversion proceeded through a succinimide intermediate. Significant deamidation is associated with the presence of SNG motif and confirms results achieved previously on model peptides. Our findings highlight an essential role of the specific amino acid sequence on accelerated rate of protein deamidation. To our knowledge, this is the first time that such a dramatic change in the relative abundance of Asp and isoAsp resulting from protein deamidation process is reported.
    Źródło:
    Acta Biochimica Polonica; 2014, 61, 2; 349-357
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Desorption/ionization on silicon for small molecules: a promising alternative to MALDI TOF.
    Autorzy:
    Kraj, Agnieszka
    Dylag, Tomasz
    Gorecka-Drzazga, Anna
    Bargiel, Sylwester
    Dziuban, Jan
    Silberring, Jerzy
    Powiązania:
    https://bibliotekanauki.pl/articles/1043452.pdf
    Data publikacji:
    2003
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    proteomics
    porous silicon
    catecholamines
    peptides
    DIOS
    mass spectrometry
    desorption/ionization
    MALDI TOF
    Opis:
    A method has been developed for laser desorption/ionization of catecholamines from porous silicon. This methodology is particularly attractive for analysis of small molecules. MALDI TOF mass spectrometry, although a very sensitive technique, utilizes matrices that need to be mixed with the sample prior to their analysis. Each matrix produces its own background, particularly in the low-molecular mass region. Therefore, detection and identification of molecules below 400 Da can be difficult. Desorption/ionization of samples deposited on porous silicon does not require addition of a matrix, thus, spectra in the low-molecular mass region can be clearly readable. Here, we describe a method for the analysis of catecholamines. While MALDI TOF is superior for proteomics/peptidomics, desorption/ionization from porous silicon can extend the operating range of a mass spectrometer for studies on metabolomics (small organic molecules and their metabolites, such as chemical neurotransmitters, prostaglandins, steroids, etc.).
    Źródło:
    Acta Biochimica Polonica; 2003, 50, 3; 783-787
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Maldi – metoda do zastosowań w analizie strukturalnej polimerów
    Maldi – method for use in structural analysis of polymers
    Autorzy:
    Swinarew, B.
    Swinarew, A. S.
    Powiązania:
    https://bibliotekanauki.pl/articles/171598.pdf
    Data publikacji:
    2014
    Wydawca:
    Polskie Towarzystwo Chemiczne
    Tematy:
    polimery
    spektrometria mas z jonizacją laserową wspomaganą matrycą
    MALDI-TOF
    kopolimery
    degradacja polimeru
    polymers
    matrix-assisted laser desorption ionization mass spectrometry
    MALDI-TOF MS
    copolymers
    polymer degradation
    Opis:
    Polymers as one of the fastest growing groups of widespread use of synthetic materials are characterized by a great diversity of structures. Structural characterization of polymers generally includes: an assessment of the average molecular weight (Mn) and the molar mass distribution (PD) to determine the structure of repeating units (mers) sequence analysis of the copolymer, identification of the end groups, the detection and identification of contaminants and substances present in the composition of the polymer asa dopant. Modern mass spectrometry (MS) offers the opportunity to study the smallest structural details of macromolecular materials [1–10]. Because of the variety of potential structures of polymer analysis process is to answer a few questions by a certain pattern. The first step is to determine the chemical structure of the polymer backbone. The second step is to identify whether the chains have branching points and define the degree of branching. The third important step is to correct end groups identification, also for the detection of cyclic oligomers that can be present. The structural studies can be made by mass spectrometer using reflectron mode. In the essence, the method involves three steps. The first analysis is performed with standard mass spectrum of the sample. Then the precursor ion (parent ion) is selected, which is subjected to further analysis by MS changed voltages and reflectron mode. This paper aims to present the issues related to the detailed analysis and characterization of polymeric materials produced on a large scale. Before, for materials such as poly(propylene), poly(ethylene), poly(styrene), polycarbonate, etc., increasing demands on the mechanical and technological parameters were placed. Maintaining a high level of products is associated with a very rigorous process control of the manufacturing, processing and transportation at every stage. The optimal tool for the structural characteristics of these polymeric materials is the defense technique MALDI-TOF MS (Matrix-Assisted Laser Desorption/Ionization Time- -Of-Flight Mass Spectrometer) due to its versatility, speed and extremely high precision. Below, we present some aspects of MALDI MS analysis of polymeric materials and composites. Note, that the following literature review focuses on the recent developments in the field of preparation of the samples, to achieve high mass resolution, the identification of polymers and copolymers, the accuracy of the molar mass determination and the identification of functional end groups, sequence analysis of the copolymer.
    Źródło:
    Wiadomości Chemiczne; 2014, 68, 7-8; 645-660
    0043-5104
    2300-0295
    Pojawia się w:
    Wiadomości Chemiczne
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    A proteomics approach to identify the differential protein level in cardiac muscle of diabetic rat
    Autorzy:
    Karthik, Dhanaraj
    Vijayakumar, Ravichandran
    Pazhanichamy, Kalailingam
    Ravikumar, Sivanesan
    Powiązania:
    https://bibliotekanauki.pl/articles/1039290.pdf
    Data publikacji:
    2014
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    diabetes mellitus
    cardiac muscle proteome
    2D electrophoresis
    MALDI-TOF-MS
    phylogenetic analysis
    Opis:
    Background: Cardiovascular proteomics investigation reveals the characterization and elucidation of the novel therapeutic targets and strategies to prevent the development of heart failure associated diabetic complication by using 2DE and MS. Methods: The experimental animals were made diabetic with a single intraperitoneal injection of alloxan (150 mg/kg of bw). Albino rats were randomly divided into four individual groups: Group-I control (n=6), group-II alloxan-induced diabetic rats, untreated (n=6), group-III (n=6) and group-IV (n=6) alloxan-induced diabetic rats were treated with aqueous and ethanolic extracts of Cynodon dactylon for 15 days, respectively. Animals were euthanized to collect the heart tissues and blood samples. 2DE sample preparation, gel running and staining (n=6: each groups) were performed at the same time to avoid variation. The result of six gel images from each group were analyzed and evaluated as one match set with 2D software (P<0.05). Results: The above experiment revealed two up-regulated proteins in group-II i.e. NTF4 and ETFB. Conclusions: NTF4 is a neuro-protective agent for neuro-degenerative diseases. It will prevent diabetic secondary complications, such as diabetic polyneuropathy and cardiomyopathy. ETFB is active in the mitochondria, the energy-producing centres in cells. It is clear from the experiment that because of up-regulation of ETFB more energy is availabile and the electron transfer for heart during diabetes is possible, what leads to reduce the oxidative stress and free-radical formation. The up-regulated proteins reduced CVD that occurred just before overt hyperglycaemia due to administration of C. dactylon. This approach established the preliminary reference map for decoding cellular mechanisms linked between pathogenesis CVD and diabetes.
    Źródło:
    Acta Biochimica Polonica; 2014, 61, 2; 285-293
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Mass spectrometry based analysis of erythrocyte membrane associated proteins in chronic myeloid leukemia patients in Sri Lanka
    Autorzy:
    Kottahachchi, D. U.
    Ariyaratne, T. R.
    Jayasekera, G. A. U.
    Powiązania:
    https://bibliotekanauki.pl/articles/411827.pdf
    Data publikacji:
    2014
    Wydawca:
    Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
    Tematy:
    erythrocyte membrane associated proteins
    1-D-SDS-PAGE
    MALDI-TOF MS
    PMF
    hematological diseases
    Opis:
    The research reported in this paper was conducted to analyze erythrocyte membrane associated proteins (ERMBPs) of some of chronic myeloid leukemia (CML) patients and selected individuals of Sri Lanka employing one dimensional sodium dodecyl sulphate poly acrylamide gel electrophoresis (1D-SDS-PAGE) combined with matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI–TOF-MS). Erythrocyte membranes from blood were isolated by osmotic lysis, centrifugation and washings. ERMBPs were separated on 1D-SDS-PAGE, visualized by silver staining and the separated protein bands dissected out from the gel and were subjected to digestion by proteolytic enzyme, trypsin, and the resulting peptide mixture was analyzed by MALDI-TOF-MS. Resulting experimental peptide mass values were analyzed by peptide mass fingerprint (PMF) technique. From this analysis 10 ERMBPs including α and β spectrin, ankyrin, band 3, band 4.1, band 4.2, band 7, dematin, actin, 55 KDa erythrocyte membrane protein were identified accurately with their primary structure information. The study was able to provide some evidence for Cathepsin associated cleavage of Band 3 anion transport protein in CML patients reported previously. In addition we were able to detect changes in gel bands between healthy controls and CML patients around the area of 20 kDa in the 1 D-SDS-PAGE. It was identified as nuclear protein Dbf 4 related factor 1 isoform 2. Although erythrocytes are devoid of nuclei, such unexpected nuclear proteins have been identified in previous research. We were successful in identifying several human ERMBPs with available resources. As the identified proteins were known to be related to pathology of some of the hematological diseases, this methodology could be extended to detect the protein changes in erythrocyte membrane protein associated diseases. Therefore, this initial research would at some point lead to discovery of biomarkers to these hematological diseases in Sri Lanka.
    Źródło:
    International Letters of Chemistry, Physics and Astronomy; 2014, 19, 1; 74-86
    2299-3843
    Pojawia się w:
    International Letters of Chemistry, Physics and Astronomy
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Comparative proteomic analysis of Bombyx mori hemolymph and fat body after calorie restriction
    Autorzy:
    Chen, Huiqing
    Li, Yijia
    Chen, Keping
    Yao, Qin
    Li, Guohui
    Wang, Lin
    Powiązania:
    https://bibliotekanauki.pl/articles/1040312.pdf
    Data publikacji:
    2010
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    calorie restriction
    Bombyx mori
    two-dimensional gel electrophoresis
    proteomic analysis
    MALDI-TOF/TOF MS
    Opis:
    Calorie restriction (CR) is known to extend life span from yeast to mammals. To gain an insight into the effects of CR on growth and development of the silkworm Bombyx mori at protein level, we employed comparative proteomic approach to investigate proteomic differences of hemolymph and fat body of the silkworm larvae subjected to CR. Thirty-nine differentially expressed proteins were identified by MALDI TOF/TOF MS. Among them, 19 were from the hemolymph and 20 from the fat body. The hemolymph of the CR group contained two down-regulated and 17 up-regulated proteins, whereas the fat body contained 15 down-regulated and five up-regulated ones. These proteins belonged to those functioning in immune system, in signal transduction and apoptosis, in regulation of growth and development, and in energy metabolism. Our results suggest that CR can alter the expression of proteins related to the above four aspects, implying that these proteins may regulate life span of the silkworm through CR.
    Źródło:
    Acta Biochimica Polonica; 2010, 57, 4; 505-511
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Bacterial species identification
    Autorzy:
    Kshikhundo, Ronald
    Itumhelo, Shayalethu
    Powiązania:
    https://bibliotekanauki.pl/articles/1153736.pdf
    Data publikacji:
    2016
    Wydawca:
    Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
    Tematy:
    16S rRNA gene
    Bacteria
    Biolog
    Gram staining
    MALDI-TOF MS
    RiboPrinter
    computational tools
    fatty acids
    identification
    metagenomics
    morphology
    Opis:
    The traditional methods of bacterial identification are based on observation of either the morphology of single cells or colony characteristics. However, the adoption of newer and automated methods offers advantage in terms of rapid and reliable identification of bacterial species. The review provides a comprehensive appreciation of new and improved technologies such fatty acid profiling, sequence analysis of the 16S rRNA gene, matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF), metabolic finger profiling using BIOLOG, ribotyping, together with the computational tools employed for querying the databases that are associated with these identification tools and high throughput genomic sequencing in bacterial identification. It is evident that with the increase in the adoption of new technologies, bacterial identification is becoming easier.
    Źródło:
    World News of Natural Sciences; 2016, 3; 26-38
    2543-5426
    Pojawia się w:
    World News of Natural Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Metody identyfikacji gatunkowej grzybów z rodzaju Candida. Część II. Techniki molekularne
    Methods for species identification of genus Candida fungi. Part II. Molecular techniques
    Autorzy:
    Gnat, Sebastian
    Powiązania:
    https://bibliotekanauki.pl/articles/22326536.pdf
    Data publikacji:
    2022
    Wydawca:
    Krajowa Izba Lekarsko-Weterynaryjna
    Tematy:
    choroby grzybicze
    grzybice
    Candida
    identyfikacja gatunkowa
    metody
    metody molekularne
    test PCR
    metoda multiplex PCR
    metoda nested-PCR
    metoda real time PCR
    fluorescencyjna hybrydyzacja in situ
    spektrometria mas MALDI-TOF MS
    czynniki chorobotwórcze
    grzyby chorobotwórcze
    łańcuchowa reakcja polimerazy
    piroliza ze spektometrią mas
    identification
    molecular techniques
    MALDI-TOF MS
    Opis:
    A rapid and accurate identification of the Candida is crucial for clinical treatment of local and systemic candidiasis. Premature diagnosis of invasive fungal infections is problematic because most clinical signs are non-specific and cultures are often negative or become positive too late for the initiation of effective antifungal therapy. Therefore, studies have been performed to improve molecular techniques for the diagnosis of candidiasis. Today, molecular strategies, such as PCR and non-PCR based methods are used to complement conventional laboratory approach. They provide accurate results in much short time of 1.5–3 h. Given the high accuracy and time saving with molecular typing techniques it is likely that most of these methods could improve routine clinical laboratory identification of Candida yeast species. However, further studies are needed for standardization of such procedures. This article presents an overview and discussion on molecular identification methods in the context of their application for the diagnosis of Candida fungi. Particular attention has been focused on the advantages and limitations of the indicated methods and the possibilities of their implementation for routine use in clinical laboratories.
    Źródło:
    Życie Weterynaryjne; 2022, 97, 03; 167-174
    0137-6810
    Pojawia się w:
    Życie Weterynaryjne
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Advanced methods of bacteriological identification in a clinical microbiology laboratory
    Autorzy:
    Zukowska, M.E.
    Powiązania:
    https://bibliotekanauki.pl/articles/2098275.pdf
    Data publikacji:
    2020
    Wydawca:
    Instytut Medycyny Wsi
    Tematy:
    clinical microbiology
    molecular method
    modern method
    multiplex polymerase chain reaction
    real-time polymerase chain reaction
    next generation sequencing
    MALDI-TOF mass spectrometry
    Opis:
    Introduction and objective. Conventional, culture-based methods of bacterial identification and drug-susceptibility testing are considered the gold standard in medical microbiology. In recent years, classical microbiological methods have been supplemented with modern analytical and molecular methods. The aim of the review was to discusses the methods which have been permanently adapted to bacteriological microbiological diagnostics. Abbreviated description of the state of knowledge. Currently, PCR, as well as other nucleic acid amplification tests and sequencing techniques, are part of the standard repertoire of microbiological diagnostics. With regard to the quality and speed of pathogen identification, the introduction of mass spectrometry techniques into routine microbiological diagnostics work-up has been revolutionary. Within a short time in many laboratories, Matrix-Assisted Laser Desorption/Ionisation – Time of Flight Mass Spectrometry (MALDI TOF MS) systems have almost completely replaced conventional biochemical pathogen identification. Conclusions. Microbiological diagnostics is an indispensable element of a targeted therapy. The techniques used in the laboratory depend primarily on the laboratory’s apparatus, the costs of the analysis, as well as the sensitivity and specificity of a method. However, regardless of the culture-based methods universality, advanced techniques have permanently established themselves in diagnostics. Confident information about the detected organism and treatment possibilities in a combination with the clinical context are conducive to successful therapy. Although modern methods still require validation and close collaboration between clinicians, microbiologists and bioinformaticians, these methods, once deemed to be the future, have already arrived.
    Źródło:
    Journal of Pre-Clinical and Clinical Research; 2021, 15, 2; 68-72
    1898-2395
    Pojawia się w:
    Journal of Pre-Clinical and Clinical Research
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Prekatalizatory oligomeryzacji olefin oparte na kationach chromu(III) oraz kobaltu(II) : właściwości fizykochemiczne oraz katalityczne
    Precatalysts for olefin oligomerization based on chromium(III) and cobalt(II) cations : physicochemical and catalytic properties
    Autorzy:
    Malinowski, Jacek
    Drzeżdżon, Joanna
    Sikorski, Artur
    Jacewicz, Dagmara Elżbieta
    Powiązania:
    https://bibliotekanauki.pl/articles/2200439.pdf
    Data publikacji:
    2022
    Wydawca:
    Polskie Towarzystwo Chemiczne
    Tematy:
    kation chromu(III)
    oligomeryzacja olefin
    2-chloro-2-propen-1-ol
    aktywność katalityczna
    MALDI-TOF MS
    chromium(III)
    olefin oligomerization
    catalytic activity
    Opis:
    The introduction describes the most important facts about the development of polyolefins. This is followed by a description of the role of polyolefins and the most important applications in industry and everyday life. The paper presents new, highly active precatalysts for oligomerization of olefins. These are coordination compounds based on chromium(III) cation, anions of various polycarboxylic acids and auxiliary ligands, such as 1,10-phenanthroline or 2,2'-bipyridyl. This review presents their crystallographic structures and basic parameters describing the elementary cell. The catalytic properties of the obtained oligomerization products using chromium(III) coordination compounds by MALDI-TOF MS are described. In conclusion, the presented precatalysts are compared with others described in the literature.
    Źródło:
    Wiadomości Chemiczne; 2022, 76, 11-12; 943--962
    0043-5104
    2300-0295
    Pojawia się w:
    Wiadomości Chemiczne
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Method for mass spectrometry spectrum deisotoping based on fuzzy inference systems
    Autorzy:
    Glodek, Anna
    Polańska, Joanna
    Powiązania:
    https://bibliotekanauki.pl/articles/747419.pdf
    Data publikacji:
    2018
    Wydawca:
    Polskie Towarzystwo Matematyczne
    Tematy:
    fuzzy logic, fuzzy inference systems, deisotoping, mass spectrometry, algorithms, proteomics, applied mathematics
    fuzzy logic, fuzzy inference systems, deisotoping, mass spectrometry, algorithms, MALDI ToF
    Opis:
    Celem niniejszego artykułu jest omówienie zaproponowanego algorytmu do identyfikacji obwiedni izotopowych, opartego na teorii systemów rozmytych. Obecnie proteomika jest ściśle powiązana z chorobami nowotworowymi. Dlatego też bardzo ważne jest precyzyjne zidentyfikowanie białek znajdujących się w obszarze raka; stosowana jest w tym celu spektrometria masowa. Jedną z technik spektrometrii masowej jest MALDI. Otrzymane dane będące widmem masowym składają się ze stosunku masy do ładunku jonów oraz intensywnosci pików. W celu przetworzenia danych, usunięcia szumu, linii bazowej etc. stosuje się przetwarzanie wstępne. Identyfikacja obwiedni izotopowych jest częścią procesu przetwarzania wstępnego w proteomice. Polega ona na identyfikacji izotopów wchodzących w skład obwiedni izotopowej, a także pozwala na zredukowanie wymiaru danych. Istnieje wiele algorytmów do identyfikacji obwiedni izotopowej, jednak każdy z nich dedykowany jest dla innego rodzaju techniki spektrometrii masowej (MALDI, LC-MS, ESI, etc.) bądź dla konkretnego rodzaju cząsteczek. Dlatego też zaproponowany algorytm został oparty na teorii systemów rozmytych, a reguły wnioskowania zostały oparte na wieloletnich doświadczeniach eksperta w dziedzinie spektrometrii masowej. Przetestowany był on na danych uzyskanych z Instytutu Onkologii im. Marii Skłodowskiej-Curie w Gliwicach, pochodzących z badań nad rakiem głowy i szyi dla losowo wybranej grupy peptydów i lipidów. Wyniki autorskiego algorytmu do identyfikacji obwiedni izotopowych porównano z jedną z istniejących metod do identyfikacji obwiedni izotopowych.
    Nowadays, mass spectrometry is widely used in proteomics for confident and precise identification of the protein. One of the most important steps in the signal analysis is deisotoping because some peaks in the spectrum are not the unique compound, but there are members of an isotopic envelope. Although the mass spectrometry is present in proteomics for a long time already, the problem of isotope peaks identification is not solved yet. The existing algorithms, usually designed for the particular type of spectrometer, are semi-supervised and do not give satisfactory results. We propose a new algorithm based on fuzzy inference systems that can accurately identify the isotopic envelopes in the spectrum of the complex structure.
    Źródło:
    Mathematica Applicanda; 2018, 46, 1
    1730-2668
    2299-4009
    Pojawia się w:
    Mathematica Applicanda
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    The application of MALDI-TOF MS for dermatophyte identification
    Autorzy:
    Dabrowska, I.
    Dworecka-Kaszak, B.
    Powiązania:
    https://bibliotekanauki.pl/articles/6182.pdf
    Data publikacji:
    2014
    Wydawca:
    Polskie Towarzystwo Parazytologiczne
    Tematy:
    application
    MALDI-TOF MS zob.matrix-assisted laser desorption-ionization time-of-flight mass spectrometry
    matrix-assisted laser desorption-ionization time-of-flight mass spectrometry
    dermatophyte
    identification
    detection
    fungi
    mammal
    skin disease
    hair
    nail
    Opis:
    Dermatophytes are keratinolytic fungi responsible for a wide variety of diseases of the skin, nails and hair of mammals. Their identification is often complicated, labor-intensive and time consuming due to the high degree of intra-species morphological similarity, and also requires scientific knowledge and practice. The aim of this study was to demonstrate that MALDI-TOF MS technique may be a faster and more sophisticated method useful for the identification of dermatophytes and mycoses in general.
    Źródło:
    Annals of Parasitology; 2014, 60, 3
    0043-5163
    Pojawia się w:
    Annals of Parasitology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Analiza syntetycznych polimerów biodegradowalnych metodą spekrometrii mas z udziałem matrycy (MALDI-TOF) kontra pomiary za pomocą wzmocnienia laserowej desorpcji/jonizacji (ELDI-TOF) z wykorzystaniem parylenu
    Analyses of synthetic biodegradable polymers by matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) versus enhanced laser desorption/ionization (ELDI-TOF) mass spectrometry using the parylene surface
    Autorzy:
    Miksa, B.
    Potrzebowski, M. J.
    Powiązania:
    https://bibliotekanauki.pl/articles/278479.pdf
    Data publikacji:
    2014
    Wydawca:
    Sieć Badawcza Łukasiewicz - Instytut Inżynierii Materiałów Polimerowych i Barwników
    Tematy:
    polimer biodegradowalny
    polimer syntetyczny
    metoda analityczna
    metoda spektroskopowa
    spektrometria mas
    MS
    poli(L-laktyd)
    PLLA
    matryca jonowa
    MALDI-TOF
    ELDI-TOF
    parylen
    biodegradable polymer
    synthetic polymer
    analytical method
    spectroscopic method
    mass spectrometry
    poly(L-lactide)
    ionic matrix
    parylene
    Opis:
    Metody spekrometrii mas w tym MALDI-TOF (matrix-assisted laser desorption/ionization time-of-flight) umożliwiają analizę mas cząsteczkowych polimerów syntetycznych (w tym biodegradowalnych) przez dobranie matrycy ułatwiającej ich jonizację lub jak w przypadku ELDI (enhanced laser desorption/ionization) w wyniku zastosowania modyfikowanej powierzchni ułatwiającej jonizację próbek. Ciecze jonowe będące solami N,N-diizopropyloetyloaminy (DEA) oraz kwasów: 2,5-dihydroksybenzoesowego (DHB), 3-indoloakrylowego (IAA) oraz 2-(4-hydroxyfenylazo)benzoesowego (HABA) znalazły zastosowanie jako matryce jonowe do wyznaczania ciężarów cząsteczkowych polilaktydów o Mw ≥ 4000 metodą MALDI-TOF. Polimery o niskich masach molowych ≤ 2000 mogą być łatwo analizowane bez udziału matrycy na powierzchni płytki pokrytej parylenem-N (poli(p-ksylylenem), PPX), który ułatwia proces laserowej desorpcji/jonizacji próbki (LDI) eliminując sygnały pochodzące od matrycy, zakłócające analizę widma masowego związków o niskich - zbliżonych do matrycy ciężarach cząsteczkowych. Zastosowanie parylenu-N do pokrywania powierzchni płytek poddanych silnej energii lasera wywołującego jonizację analizowanej próbki, nie powoduje pojawienie się dodatkowych sygnałów wynikających z obecności parylenu w widmie masowym, zakłócających interpretację otrzymanych ciężarów cząsteczkowych polilaktydu.
    Mass spectrometry MALDI-TOF (matrix-assisted laser desorption/ionization time-of-flight) enables spectral analysis of synthetic biodegradable polymers with the addition of ammonium salts using ionic matrices from N,N-diisopropylethylamine (DEA) and DHB (2,5-dihydroxybenzoic acid), IAA (3-indoleacrylic acid), and HABA (2-(4-hydroxyphenylazo) benzoic acid. However, the MALDI mass spectra of polymers of molecular mass up to 1000 Da are often heavily populated with peaks originating from the matrix and matrix secondary reaction products that introduce noise, making it difficult to interpret peaks in this region of the mass spectrum. Thus, polymers of molecular masses up to a few kDa can be analyzed, without the use of a matrix, by direct laser desorption/ionization (LDI). We used ELDI-TOF (enhanced laser desorption/ionization time-of-flight) for the rapid characterization of molecules directly from the support covered with a thin layer of parylen-N (poly(p-ksylylenes)). Hydrophobic parylen used as a support on the target plate influenced on decreasing the area size of the droplets, thus concentrating the spot including a sample of the polymer.
    Źródło:
    Przetwórstwo Tworzyw; 2014, [R.] 20, nr 1 (157), 1 (157); 50-55
    1429-0472
    Pojawia się w:
    Przetwórstwo Tworzyw
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
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