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Tytuł:
Chromatographic fractionation of betacyanins from flowers of Gomphrena globosa
Autorzy:
Szot, D.
Starzak, K.
Skopińska, A.
Wybraniec, S.
Powiązania:
https://bibliotekanauki.pl/articles/115427.pdf
Data publikacji:
2015
Wydawca:
Fundacja na Rzecz Młodych Naukowców
Tematy:
betacyanins
Gomphrena globosa
plant pigments
LC-MS
betacyjaniny
Gomfrena kulista
barwniki roślinne
Opis:
In this study, a chromatographic fractionation of betacyanin pigments from extract of purple Gomphrena globosa petals was performed by preparative high performance liquid chromatography (prep-HPLC). The particular betacyanins in each collected fractions were tentatively identified by chromatography with optical amd mass spetrometric detection (LC-DAD-ESI-MS). Betacyanins are natural pigments, which are confirmed to have an antioxidant activity. It was reported that betacyanins can prevent civilization diseases, because of an ability of free radicals scavenging. Betacyanins are present in tissue of plants from Amaranthaceae family, e.g. in petals of Gomphrena globosa. Preparative separation of these particular pigments is difficult, because they are structurally very similar to each other and tend to coelute. However, in this study, fractions, containing a dominant amount of the principal pigments of Gomphrena globosa inflorescences, were obtained successfully. In the first fractions, two isomeric pigments assigned to gomphrenin I and isogomphrenin I were detected. The fractions of significant amounts of gomphrenin III and isogomphrenin III were obtained separately with sufficient purity. The presence of other, minor gomphrenin-type betacyanins were also confirmed as: gomphrenin II, cis-isomer of gomphrenin II and sinapoyl-gomphrenin I as well as their 15S-diastereomers. Moreover, in other fractions, various unknown pigments were detected.
Źródło:
Challenges of Modern Technology; 2015, 6, 2; 19-23
2082-2863
2353-4419
Pojawia się w:
Challenges of Modern Technology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Effect of Cu (II) Cations on 2-Decarboxy-betanin Stability in Aqueous-Organic Solutions
Autorzy:
Skopińska, A.
Szot, D.
Starzak, K.
Wybraniec, S.
Powiązania:
https://bibliotekanauki.pl/articles/115534.pdf
Data publikacji:
2015
Wydawca:
Fundacja na Rzecz Młodych Naukowców
Tematy:
betacyanins
2-decarboxy-betanin
copper ions
betalain stability
Beta vulgaris L.
betacyjaniny
2-dekarboksy-betanina
jony miedzi
stabilność betalainy
Opis:
Several important factors, such us pH, exposure to light, oxygen or temperature affect significantly the betalain stability. In particular, the heavy metal ions exert negative effect on stability of betalain colorants and accelerate pigment decomposition. Trace amounts of metal ions may be present in the food products, as well as in food packaging, leading to undesirable colour changes or even discoloration of betalainic foodstuffs. Decarboxylated betalains, such as 2-decarboxy-betanin, generated, e.g. during betalain thermal treatment, may exhibit greater stability than parent pigments. Moreover, obtained derivatives retain attractive color, making them the promising material for study of pigment stability. An effect of Cu2+ cations as the most degradative metal ions on 2-decarboxy-betanin stability was investigated in ethanolic and methanolic solutions. The increase of the concentration of copper cations has a negative impact on stability of 2-decarboxy-betanin. The cations of Cu (II) in the presence of organic solvents induce the pigment degradation, while the increase of the concentration of organic solvents enhances the pigment decomposition. The main degradation products of the 2-decarboxy-betanin are compounds possessing absorption maxima at λmax ca. 430 nm. These studies allow obtaining the information needed for proper isolation and treatment of pigments, as well as proper storage of products containing betalains.
Źródło:
Challenges of Modern Technology; 2015, 6, 3; 24-29
2082-2863
2353-4419
Pojawia się w:
Challenges of Modern Technology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Enzymatic oxidation of neobetanin monitored by liquid chromatography with mass spectrometric detection
Autorzy:
Starzak, K.
Szot, D.
Skopińska, A.
Swiergosz, T.
Wybraniec, S.
Powiązania:
https://bibliotekanauki.pl/articles/115945.pdf
Data publikacji:
2016
Wydawca:
Fundacja na Rzecz Młodych Naukowców
Tematy:
neobetanin
betalains
betacyanins
horseradish peroxidase
oxidation
enzymatic
spectrophotometry
mass spectrometry
chromatography
neobetanina
betalainy
betacyjaniny
peroksydaza chrzanowa
enzymatyczne utlenianie
spektrofotometria
spektrometria masowa
chromatografia
Opis:
The aim of this study was monitoring of enzymatic oxidation of neobetanin, an interesting type of betalains which is a partially oxidized betacyanin. As it belongs to betalains, it is water soluble and non-toxic, but a presence of a few functional groups makes it very reactive. Oxidation reactions were performed using horseradish peroxidase followed by spectrophotometric and mass spectrometric detection (LC-DAD-ESI-MS/MS) of obtained products. Enzymatic oxidation of neobetanin leads to a formation of new decarboxy- and dehydro-derivatives. The main identified oxidation product is 2-decarboxy-2,3-dehydroneobetanin. Searching for all formed oxidation products is extremely important for elucidation of the betalains oxidation mechanism.
Źródło:
Challenges of Modern Technology; 2016, 7, 1; 29-31
2082-2863
2353-4419
Pojawia się w:
Challenges of Modern Technology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
High salt-solvent systems in separation of betanin and its derivatives from red beet (Beta vulgaris L.) by high-performance countercurrent chromatography (HPCCC)
Autorzy:
Spórna-Kucab, A.
Ignatova, S,
Garrard, I.
Wybraniec, S.
Powiązania:
https://bibliotekanauki.pl/articles/115564.pdf
Data publikacji:
2012
Wydawca:
Fundacja na Rzecz Młodych Naukowców
Tematy:
betanin
betalains
betacyanins
countercurrent chromatography
Beta vulgaris L.
Opis:
A study on a separation of betanin and its decarboxy- and dehydro-derivatives obtained from red beet roots (Beta vulgaris L.) using analytical high-performance countercurrent chromatography (HPCCC — Dynamic Extractions Ltd., UK) was performed. The HPCCC process was accomplished in the ‘tail to head’ mode with three highly polar solvent systems with high salt concentrations: 1-propanol-acetonitrile-saturated ammonium sulphate-water (v/v/v/v, 1:0.5:1.2:1); ethanol-acetonitrile-1- propanol-saturated ammonium sulphate-water (v/v/v/v/v, 0.5:0.5:0.5:1.2:1) and ethanol-1-butanol-acetonitrile-saturated ammonium sulphate-water (volume ratio), 0.5:0.5:0.5:1.2:1). HPLC analysis was performed in a conventional reversed phase mode with diode-array (DAD) detection to characterize the composition of obtained fractions. The applied solvent systems enabled the separation of the betalain pigments with high efficiency for the first time. In the mode of separation selected, the more hydrophobic compounds eluted first as expected. Moreover, for the first time, the applied HPCCC solvent systems generated a separation of 2-decarboxy-betanin from 17- and 2,17-bidecarboxy-betanin as well as from neobetanin and betanin.
Źródło:
Challenges of Modern Technology; 2012, 3, 1; 57-61
2082-2863
2353-4419
Pojawia się w:
Challenges of Modern Technology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Influence of EDTA on stabilization of decarboxylated betalains
Autorzy:
Skopińska, A.
Starzak, K.
Wybraniec, S.
Powiązania:
https://bibliotekanauki.pl/articles/115483.pdf
Data publikacji:
2012
Wydawca:
Fundacja na Rzecz Młodych Naukowców
Tematy:
betacyanins
decarboxylation
Beta vulgaris L.
EDTA
Opis:
Betalains are known to be very sensitive to several factors, including higher temperature, therefore, the studies on their stability are essential for the food industry. In order to determine the stability of betanin derivatives during thermal treatment, a set of degradation experiments was performed, including the protective infl uence of EDTA as a chelating agent. The pigments were dissolved in aqueous solutions of acetonitrile, methanol and ethanol. The studies on thermal degradation of the pigments confi rmed their high lability in all investigated solvents. Under the infl uence of protective activity of EDTA, the rate of the degradation of the decarboxylated betalains was substantially decreased. The analysis of obtained absorption spectra of the degradation products indicated that under the infl uence of EDTA, the products retained the basic chromophoric skeleton.
Źródło:
Challenges of Modern Technology; 2012, 3, 4; 29-33
2082-2863
2353-4419
Pojawia się w:
Challenges of Modern Technology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Liquid chromatographic techniques in separation of betacyanins and their derivatives from red beet roots
Autorzy:
Spórna, A.
Stalica, P.
Jerz, G.
Szaleniec, M.
Wybraniec, S.
Powiązania:
https://bibliotekanauki.pl/articles/115819.pdf
Data publikacji:
2010
Wydawca:
Fundacja na Rzecz Młodych Naukowców
Tematy:
betanin
betalains
betacyanins
ion-pair counter-current chromatography
perfluorinated carboxylic acids
Opis:
A study on separation of betacyanins and their decarboxylated as well as dehydrogenated derivatives obtained from red beet roots (Beta vulgaris L.) in high pressure liquid chromatography (HPLC) and ion-pair high-speed counter-current chromatography (IP-HSCCC) was performed. The IP-HSCCC process was accomplished in the ‘head-to-tail’ mode in a solvent system composed of butanol — acetonitrile — water (5:1:6 v/v/v, acidifi ed with 0.7% trifl uoroacetic acid). The HPLC separation was performed in a typical reversed phase mode with mass spectrometry (ESI-MS) and diode-array (DAD) detection. The chromatographic profi les of betalains obtained in these two techniques were signifi cantly diff erent. In HPLC, the most polar compounds, like betanin and isobetanin, eluted before less polar decarboxylated and dehydrogenated derivatives (mostly degradation products of betanin). In IP-HSCCC, the dehydrogenated derivatives were eluted faster then their non-dehydrogenated analogues. It was observed for the fi rst time that betanin and neobetanin (14,15-dehydrogenated betanin), which are present in many plants containing betalains, had reversed elution orders during chromatographic separation by these two techniques.
Źródło:
Challenges of Modern Technology; 2010, 1, 1; 19-22
2082-2863
2353-4419
Pojawia się w:
Challenges of Modern Technology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Mass Spectrometric Detection of New Betalains in Mammillaria Flowers
Autorzy:
Szot, Dominika
Starzak, Karolina
Skopińska, Anna
Wybraniec, Sławomir
Powiązania:
https://bibliotekanauki.pl/articles/1034229.pdf
Data publikacji:
2015
Wydawca:
Uniwersytet Jagielloński. Wydawnictwo Uniwersytetu Jagiellońskiego
Tematy:
Cactaceae
LC-MS
Mammillaria
betacyanins
natural pigments
Opis:
Betacyanins are natural, red-violet betalain pigments which can be found in plenty of plants of the Cactaceae family. The Mammillaria is the widest genus of the Cactaceae family of which fruit was examined for betacyanins contents. As a result of the investigation, a new pigment structure, mammillarinin, was identified. Heretofore, no betacyanins of Mammillaria coronata flowers has been extensively studied. In this report, the results of betacyanin analysis by LC-DAD-ESI-MS/MS in the flower extract are presented.
Źródło:
Zeszyty Naukowe Towarzystwa Doktorantów Uniwersytetu Jagiellońskiego. Nauki Ścisłe; 2015, 10; 189-196
2082-3827
2084-977X
Pojawia się w:
Zeszyty Naukowe Towarzystwa Doktorantów Uniwersytetu Jagiellońskiego. Nauki Ścisłe
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Profiles of products generated during betacyanins reduction by 2-methylpyridine borane
Autorzy:
Szot, D.
Starzak, K.
Skopińska, A.
Wybraniec, S.
Powiązania:
https://bibliotekanauki.pl/articles/115550.pdf
Data publikacji:
2015
Wydawca:
Fundacja na Rzecz Młodych Naukowców
Tematy:
betanina
neobetanina
betacyjaniny
redukcja
2-2-metylopirydyna boranu
LC-MS
betanin
neobetanin
betacyanins
reduction
2-methylpyridine borane
Opis:
Betanin and neobetanin (14,15-dehydrogenated betanin), natural betacyanin pigments were subjected to a gentle reduction by 2-methylpyridine borane. During each reduction reaction, the visible spectra were collected. The results were tentatively confirmed by LC-DAD-ESI-MS. In comparison to a previous study with sodium borohydride, various types of products were obtained. Depending on pH, one or two double bonds are reduced. Interestingly, a preliminary decarboxylation of the pigments is also noticed. Betacyanins are plant red-violet pigments applied as colorants in food and pharmaceutical industry which are proven to have an antioxidant activity. They are confirmed to be beneficial for human health as free radical scavengers. Therefore, betacyanins can prevent from many diseases which would be induced by an excessive accumulation of free radicals in the human body. There are more and more reports of their pro-health properties. However, the mechanism of betacyanins oxidation remains not completely elucidated. It is postulated that the rearrangement in the chromophoric system is a significant part of this mechanism. The products obtained as a result of reduction of betanin and neobetanin can be useful in oxidation investigations. Betacyanins with partially destroyed chromophoric system by reduction of the double bonds would by perfect models for further studies on their oxidation.
Źródło:
Challenges of Modern Technology; 2015, 6, 4; 19-22
2082-2863
2353-4419
Pojawia się w:
Challenges of Modern Technology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Reactions of decarboxylated betanins under influence of halogen light
Autorzy:
Skopinska, A.
Szot, D.
Starzak, K.
Wybraniec, S.
Powiązania:
https://bibliotekanauki.pl/articles/115710.pdf
Data publikacji:
2014
Wydawca:
Fundacja na Rzecz Młodych Naukowców
Tematy:
betanin
decarboxylated betacyanins
photolability
mass spectrometry
betanina
dekarboksylacja betacyjaniny
spektrometria masowa
Opis:
Betalains as natural plant pigments are very sensitive to several factors, including light, therefore, studies on stability of their derivatives which retain their attractive color, are crucial for their potential application in pharmaceutical, food or cosmetic industries. An effect of irradiation by halogen light on reactivity of main decarboxylated derivatives of betacyanins (2-decarboxy-betanin, 17-decarboxy-betanin and 2,17-decarboxy-betanin) was investigated in aqueous and organic-aqueous solutions: 50% (v/v) acetonitrile, 50% (v/v) methanol and 50% (v/v) ethanol, at pH range 3-8. The double decarboxylation of betanin (at C-2 and C-17 position of carbon) significantly increases the stability of this pigment in the acidic media. As a result of pigments reactions induced by light, some betacyanin derivatives with different decarboxylation and dehydrogenation levels were identified by chromatography with tandem mass spectrometry (LC-MS/MS) and diode array detection (LC-DAD).
Źródło:
Challenges of Modern Technology; 2014, 5, 3; 37-43
2082-2863
2353-4419
Pojawia się w:
Challenges of Modern Technology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Reduction of Selected Betacyanins
Autorzy:
SZOT, DOMINIKA
STARZAK, KAROLINA
SKOPIŃSKA, ANNA
WYBRANIEC, SŁAWOMIR
Powiązania:
https://bibliotekanauki.pl/articles/1034140.pdf
Data publikacji:
2014
Wydawca:
Uniwersytet Jagielloński. Wydawnictwo Uniwersytetu Jagiellońskiego
Tematy:
1
7-diazaheptamethin
LC-MS
betanin
chromophore
decarboxy-betacyanins
reduction
Opis:
Betacyanins are conjugates of betalamic acid and glycosylated cyclo-DOPA which bring about the creation of a specific chromophoric system, the 1,7-dia- zaheptamethin chain. Betanin, a betacyanin pigment from red beet (Beta vulga- ris L.), as well as its decarboxylated derivatives were subjected to reduction with the use of NaBH4. The pigment structures contain a vinyl linker which is postu- lated to be protonated during reaction with NaBH4. In this study, 17-decarboxy- betanin and 2,17-bidecarboxy-betanin were obtained from betanin, which had been previously isolated from red beet root (Beta vulgaris L.). Purified pigments were tested in the presence of the reduction reagent while looking for the prod- ucts of the reaction identified by LC-MS.
Źródło:
Zeszyty Naukowe Towarzystwa Doktorantów Uniwersytetu Jagiellońskiego. Nauki Ścisłe; 2014, 8; 265-273
2082-3827
2084-977X
Pojawia się w:
Zeszyty Naukowe Towarzystwa Doktorantów Uniwersytetu Jagiellońskiego. Nauki Ścisłe
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Research on dehydrogenation of gomphrenin in heated ammonia solutions of organic solvents
Autorzy:
Szmyr, N.
Wybraniec, S.
Powiązania:
https://bibliotekanauki.pl/articles/115847.pdf
Data publikacji:
2017
Wydawca:
Fundacja na Rzecz Młodych Naukowców
Tematy:
Gomphrena globosa
Betalains
Betacyanins
Gomphrenin
LC-DAD-ESI-MS
dehydrogenation
decarboxylation
Gomfrena kulista
betalainy
betacyjaniny
gomphereniny
odwodnienie
dekarboksylacja
Opis:
In this report, the influence of ammonia on dehydrogenation of gomphrenin pigment, isolated from purple inflorescences of Gomphrena globosa L., in selected organic solvents (methanol, ethanol, acetone and acetonitrile) at 40°C is presented. Betacyanins are water-soluble, vacuolar plant pigments. Due to their numerous pro-health properties, they can be applied in the pharmaceutical industry and as food colorants. However, as most of natural products, they are less stable than synthetic dyes, therefore, further studies need to be carried out on their stability, but also on determination of their degradation products as well as degradation mechanisms. For the experiments of the ammonia treatment, prolonged isolation of gomphrenin from the plant material including extraction, preliminary purification, preparative HPLC fractionation, concentration and liofilization were performed. The tested pigment dissolved in organic solvents was treated by ammonia and the samples for analysis were taken during and after reaction. For identification of obtained derivatives of gomphrenin, high-performance liquid chromatography with diode-array and mass spectrometric detection (LC-DAD-ESI-MS) was performed. As a result of heat processing, the mixture of decarboxylated and dehydrogenated gomphrenin derivatives was detected. Based on the experimental results, it can be stated that gomphrenin is characterized by the greatest stability in methanolic solutions while it is the most labile in the acetonitrilic ones.
Źródło:
Challenges of Modern Technology; 2017, 8, 1; 8-12
2082-2863
2353-4419
Pojawia się w:
Challenges of Modern Technology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Research on stability of gomphrenin pigments influenced by Cu2+ ions
Autorzy:
Szmyr, N.
Wybraniec, S.
Powiązania:
https://bibliotekanauki.pl/articles/115831.pdf
Data publikacji:
2017
Wydawca:
Fundacja na Rzecz Młodych Naukowców
Tematy:
Gomphrena globosa
Betalains
Betacyanins
Gomphrenin
LC-DAD-ESI-MS
dehydrogenation
decarboxylation
Gomfrena kulista
betalainy
betacyjaniny
gomphereniny
odwodnienie
dekarboksylacja
Opis:
This paper concerns the results of dehydrogenation of gomphrenin pigments isolated from the purple flowers of Gomphrena globosa L. depending on pH and Cu2+ activity. Betalains are the plant dyes divided into red-violet betacyanins and yellow-orange betaxanthins. These compounds show prohealth properties proven by numerous studies [1]. Isolation of gomphrenins from the plant material was performed in the following stages: preparative extraction, filtration on a silica layer, preliminary purification and concentration on a bed of strong anion exchanger, preparative chromatographic fractionation and sample liofilization. The dehydrogenation studies of gomphrenin I and II were carried out in aqueous solutions (pH 3, 5 and 7) catalyzed by Cu2+ ions addition. The resulting product mixtures of dehydrogenated and decarboxylated gomphrenin derivatives were monitored by high-performance liquid chromatography with diode-array and mass spectrometric detection (LC-DAD-ESI-MS). Based on the results, it can be stated that decarboxylated and dehydrogenated gomphrenin II derivatives are more stable than gomphrenin I derivatives, presumably due to acylation of the glucose moiety. The highest levels of gomphrenin I derivatives were observed at pH 7, however, they were mainly monodecarboxylated ones. At pH 3, significant fraction was represented by tridecarboxylated and dehydrogenated derivatives. The lowest yield of the degradation products was indicated at pH 5.
Źródło:
Challenges of Modern Technology; 2017, 8, 1; 13-18
2082-2863
2353-4419
Pojawia się w:
Challenges of Modern Technology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Semi-synthesis of red beet betacyanin ethyl-esters by esterification
Autorzy:
Tuwalska, D.
Starzak, K.
Szot, D.
Wybraniec, S.
Winterhalter, P.
Jerz, G.
Powiązania:
https://bibliotekanauki.pl/articles/115735.pdf
Data publikacji:
2014
Wydawca:
Fundacja na Rzecz Młodych Naukowców
Tematy:
betacyanin ethyl-esters
betalains
betacyanins
betanin
betanidin
esterification
estry etylowe
betalainy
betacyjaniny
betanina
betanidyna
estryfikacja
Opis:
Red beet pigments recovered from Beta vulgaris L. (Chenopodiaceae), mainly consist of purple betacyanins, such as 15R/15S-betanin. Currently,this pigment class is intensively investigated in respect of stability in food systems. Quite popular is the use of red beet extracts in dairy products such as yoghurts, and ice-creams. So far, there are no issues of toxicity using these natural pigments in food – so they seem to be a very good alternative to replace synthetic dyes which had been frequently discussed for negative side-effects to human health. Betacyanins, the glycosidation products of betanidin are very polar and therefore water soluble pigments, which are biosynthetically derived from condensation of cyclo-DOPA and betalamic acid. The significant polarity of betacyanins requires the use of ion-pair reagents such as trifluoro-acetic acid (TFA) or other homologue fluorinated additives under standard separation conditions on larger scale in spiral coil countercurrent chromatography (spCCC). In our synthetic experiment, the polarity of the natural betacyanins was reduced. This omitted the use of toxic per-fluoro ion-pair reagents. The three carboxylic groups of the betanin pigment backbone were the target functional groups of the derivatisation. In semi-synthesis, fortified red beet pigment extract was esterified with water-free ethanolic hydrogen chloride solution. The reaction was carried out at ambient temperature (7 days) to yield a mixture of betacyanin mono-, di- and tri-ethyl-esters, and their epimeric forms, as well as the respective betanidin-ethyl-esters. The time course of the reaction mixture was monitored by analytical C18-HPLC with ESI-DAD-MS/MS detection. The detected molecular weights of the pigments confirmed the presence of expected products. To the best of our knowledge, the presence of betalains containing ethyl-ester groups in biological samples have not been studied before.
Źródło:
Challenges of Modern Technology; 2014, 5, 3; 27-31
2082-2863
2353-4419
Pojawia się w:
Challenges of Modern Technology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Separation of betalains by gradient high-speed counter-current chromatography
Autorzy:
Spórna-Kucab, A.
Udziela, D.
Sobocińska, M.
Szot, D.
Wybraniec, S.
Powiązania:
https://bibliotekanauki.pl/articles/115813.pdf
Data publikacji:
2016
Wydawca:
Fundacja na Rzecz Młodych Naukowców
Tematy:
betanin
betalains
betacyanins
high-speed counter-current chromatography
Beta vulgaris L.
betanina
betalainy
betacyjaniny
HSCCC
Opis:
A study on separation of betalain mixture obtained from red beet juice (Beta vulgaris L.) by analytical high-speed counter-current chromatography (HSCCC) was performed. The extract was obtained by thermal treatment of acidified red beet juice for 30 min in 85 °C. The pigment mixture consisted of betanin/isobetanin as well as their decarboxy- and dehydro-derivatives. The HSCCC process was accomplished in the ‘tail to head’ mode with two polar solvent systems containing salt: BuOH-EtOH-NaClsolution-H2OH3PO4 (1300:700-1000:1300:700:2.5-5.5 (system I), 1300:200-400:1300:700:2.5-4.5 (system II); v/v/v/v/v). The retention of the stationary phase was 73% (system I) and 79% (system II). The mobile phase was pumped at 2 ml/min flow rate. HPLC-DAD-ESI-MS analyses were performed in reversed phase mode for the obtained HSCCC fractions and crude extract. The solvent systems enabled separation of betanin and decarboxy-betanins (system I and II) as well as neobetanin (system II). Additionally, some pure fractions of 17-decarboxy-betanin and 2,17-bidecarboxy-betanin were obtained in system II.
Źródło:
Challenges of Modern Technology; 2016, 7, 1; 23-28
2082-2863
2353-4419
Pojawia się w:
Challenges of Modern Technology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Spectrophotometric study on betanin photodegradation
Autorzy:
Skopińska, A.
Tuwalska, D.
Wybraniec, S.
Starzak, K.
Mitka, K.
Kowalski, P.
Szaleniec, M.
Powiązania:
https://bibliotekanauki.pl/articles/115905.pdf
Data publikacji:
2012
Wydawca:
Fundacja na Rzecz Młodych Naukowców
Tematy:
betanin
betacyanins
photodegradation
Beta vulgaris L.
Opis:
The most commonly occurring betacyanin plant pigment in nature is betanin (5-O-glucoside of betanidin). Its main source is red beet root (Beta vulgaris L.), used for production of food colorants on a commercial scale [1]. Due to photo-lability of betacyanins, an eff ect of UV-irradiation on betanin degradation in various solutions containing organic solvents was investigated. Organic solvents are used frequently in many stages of pigment preparation or isolation, therefore, knowledge of betalain stability and degradation characteristics in these solutions is essential. Pigment solutions at a concentration of 1 mg/ml were prepared in diff erent solvents: water as well as aqueous solutions of 50% (v/v) acetonitrile, 50% (v/v) methanol or 50% (v/v) ethanol, at the pH range 3–8. Acetate, phosphate and citrate buff ers were used for the experiments. As a result of the pigment photo-decomposition, numerous decarboxylated and dehydrogenated derivatives were formed. Spectrophotometric studies enabled determination of the pigment retention (percentage of the pigment residue relative to its initial concentration after decomposition time). The studies demonstrated betanin high lability in all solvents, not only at extreme pH values, but also in the middle pH range which, in general, provides higher stability of betalains.
Źródło:
Challenges of Modern Technology; 2012, 3, 4; 34-38
2082-2863
2353-4419
Pojawia się w:
Challenges of Modern Technology
Dostawca treści:
Biblioteka Nauki
Artykuł
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