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Tytuł:
Synthesis, antiprotozoal and antibacterial activity of nitro- and halogeno-substituted benzimidazole derivatives.
Autorzy:
Kazimierczuk, Zygmunt
Upcroft, Jacqueline
Upcroft, Peter
Górska, Agata
Starościak, Bohdan
Laudy, Agnieszka
Powiązania:
https://bibliotekanauki.pl/articles/1043825.pdf
Data publikacji:
2002
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
antibacterial activity
antiprotozoal activity
benzimidazoles
Opis:
Two series of benzimidazole derivatives were sythesised. The first one was based on 5,6-dinitrobenzimidazole, the second one comprises 2-thioalkyl- and thioaryl-substituted modified benzimidazoles. Antibacterial and antiprotozoal activity of the newly obtained compounds was studied. Some thioalkyl derivatives showed remarkable activity against nosocomial strains of Stenotrophomonas malthophilia, and an activity comparable to that of metronidazole against Gram-positive and Gram-negative bacteria. Of the tested compounds, 5,6-dichloro-2-(4-nitrobenzylthio)-benzimidazole showed the most distinct antiprotozoal activity.
Źródło:
Acta Biochimica Polonica; 2002, 49, 1; 185-195
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Nanosilver - does it have only one face?
Autorzy:
Likus, Wirginia
Bajor, Grzegorz
Siemianowicz, Krzysztof
Powiązania:
https://bibliotekanauki.pl/articles/1039429.pdf
Data publikacji:
2013
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
silver nanoparticles
antibacterial activity
toxicology
Opis:
Silver nanoparticles (NPs) have at least one dimension of a particle smaller than 100 nm and contain 20-15,000 silver atoms. Due to its antibacterial activity nanosilver (NS) is used for medical purposes. NS particles can be obtained by various methods. Potentially, the best method of the NS synthesis for medical purposes is based on a brief flow of electric current between two silver electrodes placed in deionized water. It is accepted that the major antibacterial effect of silver is its partial oxidation and releasing silver ions, which interact with thiol groups of peptidoglicans of bacterial cell wall, and proteins of the cell membrane causing cell lysis. Silver ions can also bind to bacterial DNA preventing its replication and stopping synthesis of bacterial proteins. The rise in exposure to silver NPs has spurred interest into their toxicology. NS undergoes a set of biochemical transformations including accelerated oxidative dissolution in gastric acid, binding to thiol groups of serum and tissue proteins, exchange between thiol groups, sulfides and selenides, binding to selenoproroteins and photoreduction in skin to zerovalent metallic silver. Animal studies have shown that exposure to NS may lead to liver and spleen damage. NS can also stimulate an increased secretion of proinflammatory cytokines by monocytes. As a spectrum of NS applications is still growing, the complex evaluation of a safety of its use becomes an important task. This requires an elucidation of not only the influence of NS on human cells and organism, but also its biotransformation in organism and in environment.
Źródło:
Acta Biochimica Polonica; 2013, 60, 4; 495-501
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Biomaterials with antibacterial activity
Autorzy:
Wysocka, K.
Buzalewicz, I.
Wieliczko, A.
Kowal, K.
Stręk, W.
Podbielska, H.
Powiązania:
https://bibliotekanauki.pl/articles/284386.pdf
Data publikacji:
2008
Wydawca:
Akademia Górniczo-Hutnicza im. Stanisława Staszica w Krakowie. Polskie Towarzystwo Biominerałów
Tematy:
biomaterials
silver nanoparticles
antibacterial activity
Opis:
The sol-gel derived silica spheres with surfaces modified by silver nanoparticles were used to enhance the photodynamic effect. The silica nanoparticles were prepared by modified Stöber synthesis. The diameter of obtained silica spheres was ca. 100 nm. These silica spheres we used as a carrier for noble nanoparticles. It was shown that Ag-Au nanoparticles have an antibacterial activity against Escherichia coli. This effect depends on the nanoparticles concentration and it is stronger for higher concentrations. Laser irradiation enhances this effect, and starting from certain concentration it is possible to kill Escherichia coli, totally, when using laser light.
Źródło:
Engineering of Biomaterials; 2008, 11, no. 81-84; 119-121
1429-7248
Pojawia się w:
Engineering of Biomaterials
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Formation and properties of dbc/pla microfibres
Autorzy:
Biniaś, Dorota
Biniaś, Włodzimierz
Machnicka, Alicja
Janicki, Jarosław
Hałuszka, Patrycja
Powiązania:
https://bibliotekanauki.pl/articles/1034598.pdf
Data publikacji:
2017
Wydawca:
Sieć Badawcza Łukasiewicz - Polskie Towarzystwo Chitynowe
Tematy:
PLA
antibacterial activity
dibutyrylchitin
electrospinning
microfibres
Opis:
In this study, a solution of dibutyrylchitin (DBC)/polylactide (PLA) blend micro and nanofibres were successfully fabricated using blends of 2,2,2-trifluoroethanol (TFE) as solvents. Fibres were produced from the solutions by electrospinning. The DBC/PLA blend solutions in various ratios were studied for electrospinning into micro/nanofibres. The morphology of the micro and nanofibres was observed by scanning electron microscope (SEM). The biggest diameters of DBC/PLA fibres were obtained for the blended microfibres in ratios of 10/90 and 25/75. The smallest diameter was observed for pure polymers. The antibacterial properties were examined for materials obtained by electrospinning. In the experiments, materials with antibacterial properties were made. It is likely that the electrospun micro and nanofibres will be used in the native extracellular matrix for tissue engineering.
Źródło:
Progress on Chemistry and Application of Chitin and its Derivatives; 2017, 22; 5-13
1896-5644
Pojawia się w:
Progress on Chemistry and Application of Chitin and its Derivatives
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Highly developed construction of Heterostructured InWO3-InMoO3 loaded-TiO2 nanomaterial and Expanding multi-application
Autorzy:
Kamalakkannan, J.
Senthilvelan, S.
Powiązania:
https://bibliotekanauki.pl/articles/1178704.pdf
Data publikacji:
2017
Wydawca:
Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
Tematy:
HR-TEM
Photocatalytic
antibacterial activity
electrochemical study
Opis:
InWO3-InMoO3 loaded-TiO2 nanomaterial was synthesised by co-precipitation method and sonication technique. The nanomaterial was characterized by High-resolution scanning electron microscopy (HR-SEM) with elementary dispersive X-ray (EDX), High-resolution transmission electron microscopy (HR-TEM), photoluminescence spectroscopy (PL) and UV-Vis diffuse reflectance spectroscopy (DRS). The photocatalytic activity of InWO3-InMoO3 loaded-TiO2 nanomaterial was studied from the photodegradation of methyl green (MEG) and methylene blue (MB) under UV-light irradiation at 365 nm. The hydroxyl radical formation in the mechanism was confirmed by fluorescence quenching technique. The mineralization confirmed by chemical oxygen demand measurements. A flaxisible mechanism is proposed for higher catalytic activity of InWO3-InMoO3-TiO2 under UV-light for 365 nm. This catalyst was found to be stable and reusable without appreciable loss of catalytic activity up to five cycles. The InWO3- InMoO3 loaded-TiO2 nanomaterial on MEG was high photocatalytic activity that of MB so high photocatalytic activity has been discussed by first order kinetics, rate constant value and high quantum yield. This material was showed increased hydrophobicity that of TiO2 electrochemical study and antibacterial activity of the analysis was also studied.
Źródło:
World Scientific News; 2017, 67, 2; 338-364
2392-2192
Pojawia się w:
World Scientific News
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Synthesis and antimicrobial activity of substituted (E)-1-(4-fluoro-3-methylphenyl)-3-phenylprop-2-en-1-one compounds
Autorzy:
Balaji, S.
Senbagam, R.
Rajarajan, M.
Vijayakumar, R.
Manikandan, V.
Vanangamud, G.
Thirunarayanan, G.
Powiązania:
https://bibliotekanauki.pl/articles/1189942.pdf
Data publikacji:
2016
Wydawca:
Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
Tematy:
Antibacterial activity
Antifungal activity
Bacillus subtilis
vibrio cholera
Opis:
In an effort to develop antimicrobial agents, a series of substituted (E)-1-(4-fluoro-3-methylphenyl)-3-phenylprop-2-en-1-one compounds were synthesized by crossed-aldol condensation reaction of 4-fluoro-3-methyl acetophenone with various substituted benzaldehydes in the presence of sodium hydroxide. The synthesized compounds were characterized by means of their UV, IR and NMR spectral data. The synthesized substituted (E)-1-(4-fluoro-3-methylphenyl)-3-phenylprop-2-en-1-one compounds were tested for their antibacterial and antifungal activities by the Bauer-Kirby disc diffusion method.
Źródło:
World Scientific News; 2016, 52; 14-30
2392-2192
Pojawia się w:
World Scientific News
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Biological activities and chemical composition of solvent extracts of Stoechospermum marginatum (C. Agardh)
Autorzy:
Esmaeili, Akbar
Khakpoor, Marjan
Powiązania:
https://bibliotekanauki.pl/articles/1039656.pdf
Data publikacji:
2012
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
brown seaweed
antibacterial activity
Stoechospermum marginatum
extract
antioxidant activity
Opis:
The objective of this study was to evaluate the chemical composition CH3OH-CH2Cl2 (1:1) extract and biological activities of various extracts derived from the aerial parts of the brown marine alga Stoechospermum marginatum (C. Agardh). Gas chromatography (GC) and gas chromatography-mass spectroscopy (GC-MS) were used to analyze the composition of the essential oil. Total phenolics assay demonstrated a high value in hexane extract (HE), with a lower value for chloroform extract (CE), and the lowest value for methanol extract (ME). DPPH (2,2-diphenyl-1-picrylhydrazyl) assay showed that extracts of S. marginatum possess radical scavenging activity (RSA). Tests of the antioxidant property of the extracts revealed both electron and hydrogen transfer mechanisms. The antibacterial activity of the ME, CE, and HE as well as an ethanol extract was estimated against seven Gram-positive and Gram-negative bacteria. The ethanol extract showed the highest antibacterial activity, and the HE showed the lowest.
Źródło:
Acta Biochimica Polonica; 2012, 59, 4; 581-585
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Evaluation of the phenolic contents and antibacterial activity of different concentrations of Onosma chlorotricum Boiss
Autorzy:
Dousti, B.
Nabipor, F.
Powiązania:
https://bibliotekanauki.pl/articles/2096818.pdf
Data publikacji:
2021
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
phenolic compounds
flavonoids
antibacterial activity
Onosma chlorotricum
Opis:
Because of side effects and increasing resistance to antibiotics, considerable attention hasbeen given to extract sand biologically active compounds isolated from medicinal plants. In the present study, phytochemical screening and antibacterial activities of different concentrations of various extracts of Onosma chlorotricum were evaluated and compared with those of vancomycin and amikacin. Total phenolic and flavonoid contents and the antibacterial activity of methanol, n-hexane, and aqueous extracts (5 mg/ml to 0.156 mg/ml final concentration) of O. chlorotricum against Staphylococcus aureus, Pseudomonas aeruginosa, and Escherichia coli were evaluated using disc diffusion and microdilution methods. Total phenolic and flavonoid contents were determined according to the Folin-Ciocalteu and aluminum chloride colorimetric assays, respectively. The results showed that the total phenolic and flavonoid contents of these extracts ranged from 56.10 ± 0.13 to 74.12 ± 0.05 mg GAE/g dry extract and from 19.3 ± 0.6 to 23.20 ± 0.41 mg QE/g dry extract, respectively. The methanol extract with the highest phenolic and flavonoid content showed the highest antibacterial activity against all the tested bacterial strains, with the highest inhibition zone of 21 ± 0.7 mm and the lowest MIC and MBC values of 78.12 μg/ml for S. aureus. The antibacterial effects and the total phenolic content of O. chlorotricum were remarkable. The results suggest that the effects of methanol extract on wound healing could be tested in an animal model.
Źródło:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2021, 102, 4; 377-386
0860-7796
Pojawia się w:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Antibacterial Activities of Some Schiff Bases Involving Thiosemicarbazide and Ketones
Autorzy:
Shariar, S. M. S.
Jesmin, M.
Ali, M. M.
Powiązania:
https://bibliotekanauki.pl/articles/412294.pdf
Data publikacji:
2014
Wydawca:
Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
Tematy:
antibacterial activity
Minimum inhibitory concentration
Brine shrimp lethality
Opis:
Three Schiff bases derived from thiosemicarbazide and ketones (Vanillin, Benzophenone and Acetophenone) were used to study their antibacterial activities against some pathogenic bacteria by disc diffusion method. Of these, benzophenone thiosemicarbazone showed significant antibacterial activity as compared with that of Kanamycin. All these three compounds were found to possess cytotoxic effect. Minimum inhibitory concentration of these compounds was also determined.
Źródło:
International Letters of Chemistry, Physics and Astronomy; 2014, 7; 53-61
2299-3843
Pojawia się w:
International Letters of Chemistry, Physics and Astronomy
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Aktywność przeciwbakteryjna in vitro wytworzonych bioszkieł zawierających srebro
In vitro antibacterial activity of silver-containing bioglasses
Autorzy:
Ciołek, L.
Karaś, J.
Olszyna, A.
Zaczyńska, E.
Czarny, A.
Żywicka, B.
Powiązania:
https://bibliotekanauki.pl/articles/285884.pdf
Data publikacji:
2010
Wydawca:
Akademia Górniczo-Hutnicza im. Stanisława Staszica w Krakowie. Polskie Towarzystwo Biominerałów
Tematy:
bioszkło
działania przeciwbakteryjne
srebro
bioglasses
antibacterial activity
silver
Źródło:
Engineering of Biomaterials; 2010, 13, no. 99-101; 21-23
1429-7248
Pojawia się w:
Engineering of Biomaterials
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Chemical composition, antioxidant and antimicrobial activities of Moroccan species of Psidium guajava extracts
Autorzy:
Lahlou, J.
Amraoui, B.E.
El-Wahidi, M.
Bamhaoud, T.
Powiązania:
https://bibliotekanauki.pl/articles/2082864.pdf
Data publikacji:
2022
Wydawca:
Narodowy Instytut Zdrowia Publicznego. Państwowy Zakład Higieny
Tematy:
antibacterial activity
antifungal activity
polyphenol
medicinal plant
essential oil
Opis:
Background. During the recent years, appropriate attention has been paid to the oxidative stress which damages the body’s cells, proteins, and DNA. Therefore, the need of antioxidants becomes a therapeutic and preventive priority. In addition, microbial infections also constitute a public health problem. Objective. To find efficient, reliable and safe alternatives sources to synthetic antioxidants, antibiotics and antifungals drugs. Materials and methods. Extract and essential oil of Psidium guajava were screened for their antioxidant and antimicrobial activities against gram positive bacteria (Enterococcus faecalis and Staphylococcus aureus), gram negative bacteria (Citrobacter freundii, Escherichia coli and Pseudomonas sp) and fungi (Candida albicans,Candida tropicalis and Cryptococcus neoformans), as well as to determine the functional groups of phytochemicals present in the essential oil by Fourier transform infrared spectroscopy (FTIR). Results. The results indicate that P. guajava leaves extract demonstrated very high antioxidant activity and P.guajava essential oil showed the highest polyphenols content. The antioxidant capacity showed a significant negative linear correlation to total polyphenolic content (TPC) with Pearson’s correlation coefficients. P. guajava essential oil shows high antibacterial and antifungal activity against all the studied bacteria and fungi.The FTIR analysis of P. guajava essential oil showed the presence of several functional groups (ethers, esters, ketones, terpenes, alkanes, aldehydes, aromatic hydrocarbons, alcohols, and phenols). The relationship between the chemical composition and antimicrobial activity of P.guajava essential oil suggests that the attribution of its antimicrobial activity to a particular compound or a synergistic effects between its different constituents remains difficult. Conclusions. The present study demonstrated that Psidium guajava is a valuable source of active compounds with anti- oxidant and antimicrobial activities. This finding suggests the new use of the fruits and the leaves extracts of this plant in the treatment of bacterial and fungal infections, as well as for the extraction of new antioxidants. Therefore, it is necessary to be carried out in another study to identify the active(s) compound(s) in P.guajava essential oil with respect to their mechanisms and synergistic actions.
Źródło:
Roczniki Państwowego Zakładu Higieny; 2022, 73, 1; 65-77
0035-7715
Pojawia się w:
Roczniki Państwowego Zakładu Higieny
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Synthesis of silver nanoparticles by aqueous extract of Zingiber officinale and their antibacterial activities against selected species
Autorzy:
Hussain, Zawar
Jahangeer, Muhammad
ur Rahman, Shafiq
Ihsan, Tamanna
Sarwar, Abid
Ullah, Najeeb
Aziz, Tariq
Alharbi, Metab
Alshammari, Abdulrahman
Alasmari, Abdullah F.
Powiązania:
https://bibliotekanauki.pl/articles/27315653.pdf
Data publikacji:
2023
Wydawca:
Zachodniopomorski Uniwersytet Technologiczny w Szczecinie. Wydawnictwo Uczelniane ZUT w Szczecinie
Tematy:
Nanotechnology
Antibacterial activity
Silver-nanoparticles
green synthesis
Ginger
Opis:
Silver nanoparticles have special plasmonic and antibacterial characteristics that make them efficient in a variety of commercial medical applications. According to recent research, chemically synthesized silver nanoparticles are harmful even in low concentrations. It was crucial to identify appropriate synthesis methods that may have low costs and be nontoxic to the environment. Zingiber officinale (ginger) extracts used to prepare silver nanoparticles were inexpensive and environmentally friendly, and the best physicochemical characteristics were analyzed. Silver nanoparticles were characterized by using UV-visible spectroscopy, Scanning electron microscopy (SEM), and X-ray diffraction (XRD). The surface Plasmon resonance peak at 425 nm was observed using UV-Visible spectroscopy. Scanning electron microscopy observed that the nanoparticles were spherical and ranged in size from 5 to 35 nm. The XRD pattern values of 2θ: 38.2° , 46.3° , and 64.58° are used to determine the planes (111), (200), and (220). The silver nanoparticle’s existence was verified by the face-centered cubic (FCC). Silver nanoparticles were found to have antibacterial efficacy against gram-positive Staphylococcus and gram-negative bacteria such as Pseudomonas aeruginosa, Klebsiella Aerogenes, Salmonella, Staphylococcus and Escherichia coli. The antibacterial activity of silver nanoparticles was observed using the agar well diffusion (AWD) method at three different concentrations (100 μg/ ml, 75 μg/ml, and 50 μg/ml). The zone of inhibition measured against the bacterial strains pseudomonas Aeruginosa, Klebsiella aerogenes, Escherichia coli, Salmonella and Staphylococcus which were (18.4±1.25 mm, 16.9±0.74 mm, 14.8±1.25 mm), (16.8±0.96 mm, 14.6±0.76 mm, 14.0±1.15 mm), (19.7±0.76 mm, 18.2±0.66 mm, 15.4±1.15 mm), (16.6±0.67 mm, 14.2±0.23 mm, 12.8±0.78 mm) and (12±0.68 mm, 10±0.20 mm, 08±0.15 mm). These nanoparticles’ potent antibacterial properties may enable them to be employed as nanomedicines for a variety of gramnegative bacterial illness treatments.
Źródło:
Polish Journal of Chemical Technology; 2023, 25, 3; 23--30
1509-8117
1899-4741
Pojawia się w:
Polish Journal of Chemical Technology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
In vitro pharmacological interaction of caffeine and first-line antibiotics is antagonistic against clinically important bacterial pathogens
Autorzy:
Olajuyigbe, Olufunmiso
Adeoye-Isijola, Morenike
Okon, Victoria
Adedayo, Otunola
Coopoosamy, Roger
Powiązania:
https://bibliotekanauki.pl/articles/1038643.pdf
Data publikacji:
2017
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
antibacterial activity
bacterial isolates
caffeine
antibiotics
antagonistic effects
Opis:
The in vitro antibacterial activity of pure caffeine powder and its interaction with first line antibiotic against bacterial isolates were investigated with the macrobroth dilution and the checkerboard assay methods. This study showed that caffeine and the antibiotics exhibited various degrees of antibacterial activities. While caffeine had MICs ranging between 67.19 and 268.75 µg/ml, chloramphenicol was characterized by MICs between 0.98 and 31.25 µg/ml, kanamycin - 15.63-62.5 µg/ml, nalidixic acid - 0.49-250 µg/ml, erythromycin - 0.49-62.5 µg/ml, tetracycline - 1.99-62.5 µg/ml and metronidazole - 15.63-31.25 µg/ml. Combining ½ MICs and MICs of caffeine with the antibiotics as well as direct combination of caffeine and the antibiotics resulted in significant reduction of antibiotics' effectiveness. The fractional inhibitory concentration index (FICI) for the combination of ½ MICs of caffeine with different antibiotics showed antagonistic interactions with the antibiotics except kanamycin which had additive and indifferent interactions with caffeine. The FICI of the MICs of caffeine combined with antibiotics showed a reduction in the number of antagonistic interactions as chloramphenicol, nalidixic acid and erythromycin showed some indifferent interactions while kanamycin was the only antibiotic that showed indifferent interaction against all the bacterial isolates. The direct combination of caffeine and the antibiotics resulted in significant antagonistic interactions higher than in the case when caffeine, at the ½ MICs and MICs, was combined with the antibiotics. Although caffeine demonstrated significant antibacterial activity against the selected bacterial isolates, its combination with the selected antibiotics resulted in significant antagonistic interactions. Caffeine should not be combined with antibiotics as this could result in serious therapeutic failure and, possibly, drug toxicity in vivo.
Źródło:
Acta Biochimica Polonica; 2017, 64, 2; 255-263
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Synthesis, characterization and anti-bacterial activity of mix ligand complexes of copper with isonicotinic acid and different N-donor ligands
Autorzy:
Mokariya, Devang N.
Tejani, Rasik M.
Jadeja, Juvansinh J.
Shah, Manish K.
Powiązania:
https://bibliotekanauki.pl/articles/1031628.pdf
Data publikacji:
2020
Wydawca:
Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
Tematy:
Antibacterial activity
Mix ligand complexes
N-donor ligands
Opis:
Metal complexes of copper with isonicotinic acid and different N-donor ligands like N-Ethyl Piperazine, N-Methyl Piperazine and 4-Methyl Pyridine are synthesized. Complexes are characterized by different techniques like Elemental analysis, IR Spectroscopy, MASS Spectroscopy, Conductance and TGA. Complexes are screened for antimicrobial activity.
Źródło:
World Scientific News; 2020, 146; 95-109
2392-2192
Pojawia się w:
World Scientific News
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Przeciwbakteryjna aktywność modyfikowanego lizozymu wobec bakterii Proteus mirabilis i Pseudomonas fluorescens
Antibacterial activity of modified lysozyme against bacteria Proteus mirabilis and Pseudomonas fluorescens
Autorzy:
Cegielska-Radziejewska, R.
Szablewski, T.
Leśnierowski, G.
Kijowski, J.
Powiązania:
https://bibliotekanauki.pl/articles/270608.pdf
Data publikacji:
2010
Wydawca:
Centralny Ośrodek Badawczo-Rozwojowy Aparatury Badawczej i Dydaktycznej, COBRABiD
Tematy:
aktywność przeciwbakteryjna
modyfikowany lizozym
antibacterial activity
modified lysozyme
Opis:
Celem pracy była ocena aktywności przeciwbakteryjnej modyfikowanego lizozymu w stosunku do bakterii Gram-ujemnych Pseudomonas fluorescens i Proteus mirabilis. Porównano antybakteryjne działanie monomeru lizozymu i jego preparatu uzyskanego w wyniku modyfikacji termiczno-chemicznej. W otrzymanym preparacie lizozymu oznaczono udział form oligomerycznych, aktywność hydrolityczną i hydrofobowość. Stwierdzono wzrost inhibitującego działania lizozymu po modyfikacji w porównaniu z monomerem lizozymu i próbą bez dodatku enzymu w stosunku do badanych bakterii. Wykazano, że modyfikowany preparat lizozymu wykazujący aktywność przeciwbakteryjną wobec bakterii gramujemnych, charakteryzuje się wyższym udziałem form oligomerycznych jak również wyższą hydrofobowością powierzchniową.
The aim of the study was to assess the antibacterial activity of modified lysozyme in relation to Gram-negative bacteria Pseudomonas fluorescens and Proteus mirabilis. Antibacterial action of lysozyme monomer and its preparation produced as a result of thermo-chemical modification were compared. In the obtained lysozyme preparation the proportion of oligomeric forms, hydrolytic activity and hydrophobicity were determined. An increase was found in the inhibitory action of lysozyme after modification in comparison with lysozyme monomer and the sample without an addition of the enzyme in relation to the analysed bacteria. It was shown that the modified lysozyme preparation exhibiting antibacterial activity against Gram-negative bacteria is characterised by a higher proportion of oligomeric forms, as well as higher surface hydrophobicity.
Źródło:
Aparatura Badawcza i Dydaktyczna; 2010, 15, 3; 79-84
2392-1765
Pojawia się w:
Aparatura Badawcza i Dydaktyczna
Dostawca treści:
Biblioteka Nauki
Artykuł
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