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Tytuł:
Numerical and experimental investigation on nonlinear dynamic characteristics of planetary gear train
Autorzy:
Zhang, Jianwu
Guo, Han
Yu, Haisheng
Zhang, Tong
Powiązania:
https://bibliotekanauki.pl/articles/1839620.pdf
Data publikacji:
2020
Wydawca:
Polskie Towarzystwo Mechaniki Teoretycznej i Stosowanej
Tematy:
deep hybrid transmission
power split devices
lumped parameter model
dynamic instability
Opis:
A deep hybrid electric vehicle (DHEV) equipped with a Ravigneaux compound planetary gear train (PGT) encounters severe gear whine noises during acceleration in the EV drive mode. For the analysis of vibro-acoustic sources, a 5DOF lumped-parameter vibration model for the PGT dynamic system is established as well as sound pressures radiated from the transmission on a test bench are measured for data processing and recognition. By comparison between numerical and experimental analyses, natural vibration modes of the PGT are examined and high frequency modal resonances in association with the planetary gears are observed only to cause narrow band whine noises. Furthermore, a 2DOF reduced dynamic model for the planetary gears with consideration of nonlinearities such as time-varying mesh stiffness and backlash is proposed, and numerical solutions to bifurcations and dynamic instabilities of the two sets of planetary gears are obtained. It is found that nonlinear vibration behaviour of the long and short planets are major causes of shock and vibration of the hybrid transmission. Severe vibro-acoustic noises excited dominantly by the planetary gears are alleviated after implementing micro-geometry modifications to the PGT.
Źródło:
Journal of Theoretical and Applied Mechanics; 2020, 58, 4; 1009-1022
1429-2955
Pojawia się w:
Journal of Theoretical and Applied Mechanics
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Interception of a hypersonic vehicle by low-speed interceptors: novel perspectives and cost optimisation
Autorzy:
Liu, Shuangxi
Liu, Shijun
Yan, Binbin
Zhang, Tong
Zhang, Xu
Yan, Jie
Powiązania:
https://bibliotekanauki.pl/articles/2173726.pdf
Data publikacji:
2022
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
small speed ratio
hypersonic vehicle
interception performance
boundary conditions
współczynnik prędkości mały
pojazd hipersoniczny
wydajność przechwytywania
warunki brzegowe
Opis:
The conditions for accurately intercepting hypersonic vehicles by low-speed interceptors in the terminal guidance process are examined, considering the general form of a guidance scheme. First, based on the concept of the engagement geometry, three interception scenarios are established considering different manoeuvring configurations of the interceptors and hypersonic vehicle. Second, the boundary conditions for intercepting hypersonic vehicles (with speeds higher than those of the interceptors) are specified for the three scenarios, considering several factors: the speed, path angle, line-of-sight angle, and available overload of the interceptor; path angle and manoeuvrability of the hypersonic vehicle; and relative distance between the interceptor and vehicle. A series of simulations are performed to clarify the influence of each factor on the interception performance in the three interception scenarios. The challenges associated with accurately intercepting hypersonic vehicles by low-speed interceptors are summarised, and several recommendations for designing guidance laws are presented.
Źródło:
Bulletin of the Polish Academy of Sciences. Technical Sciences; 2022, 70, 6; art. no. e143537
0239-7528
Pojawia się w:
Bulletin of the Polish Academy of Sciences. Technical Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Overexpression of zeaxanthin epoxidase gene from Medicago sativa enhances the tolerance to low light in transgenic tobacco
Autorzy:
Cao, Yuman
Zhang, Zhiqiang
Zhang, Tong
You, Zhang
Geng, Jincai
Wang, Yafang
Hu, Tianming
Yang, Peizhi
Powiązania:
https://bibliotekanauki.pl/articles/1038375.pdf
Data publikacji:
2018
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
Medicago sativa
low-light tolerance
zeaxanthin epoxidase
overexpression
Opis:
Zeaxanthin epoxidase (ZEP) plays an important role in xanthophyll cycle which is a process closely related to photosynthesis. However, an impact of ZEP on low-light stress has not been studied. In this study, the functions of an alfalfa (Medicago sativa) zeaxanthin epoxidase gene, MsZEP, in response to low-light stress were investigated by heterologous expression in tobacco (Nicotiana tabacum). Under normal light conditions, the measured parameters were not significantly different between transgenic and wild-type (WT) plants except for non-photochemical quenching value and chlorophyll a content. However, the differences were detected under low-light stress. We found that MsZEP-overexpression tobacco grew faster than WT (p≤0.05). The leaf fresh weight and leaf area of transgenic plants were significantly higher, and the number of stomata was greater in MsZEP-overexpression tobacco. As for photosynthetic characteristics, quantum yield of PSII (ΦPSII) and maximal photochemical efficiency of PSII (Fv/Fm) were not significantly different, whereas non-photochemical quenching (NPQ), net photosynthetic rate (Pn), stomatal conductance (Gs) and transpiration rate (Tr) of MsZEP-overexpression tobacco were significantly higher than in WT plants. However, no significant difference was detected between the two types of tobacco in chlorophyll and carotenoids content. In conclusion, MsZEP can improve the ability of tobacco to withstand low-light stress, which might be due to its stronger photosynthetic activity and the improvement of stomatal density under low light.
Źródło:
Acta Biochimica Polonica; 2018, 65, 3; 431-435
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Observer-based fault-tolerant control against sensor failures for fuzzy systems with time delays
Autorzy:
Tong, S.
Yang, G.
Zhang, W.
Powiązania:
https://bibliotekanauki.pl/articles/930168.pdf
Data publikacji:
2011
Wydawca:
Uniwersytet Zielonogórski. Oficyna Wydawnicza
Tematy:
sterowanie rozmyte
sterowanie z opóźnieniem
obserwator stanu
sterowanie tolerujące uszkodzenia
liniowa nierówność macierzowa
analiza stateczności
fuzzy time delay systems
sensor faults
state observer
fault tolerant control
linear matrix inequalities
stability analysis
Opis:
This paper addresses the problems of robust fault estimation and fault-tolerant control for Takagi-Sugeno (T-S) fuzzy systems with time delays and unknown sensor faults. A fuzzy augmented state and fault observer is designed to achieve the system state and sensor fault estimates simultaneously. Furthermore, based on the information of on-line fault estimates, an observer-based dynamic output feedback fault-tolerant controller is developed to compensate for the effect of faults by stabilizing the resulting closed-loop system. Sufficient conditions for the existence of both a state observer and a fault-tolerant controller are given in terms of linear matrix inequalities. A simulation example is given to illustrate the effectiveness of the proposed approach.
Źródło:
International Journal of Applied Mathematics and Computer Science; 2011, 21, 4; 617-627
1641-876X
2083-8492
Pojawia się w:
International Journal of Applied Mathematics and Computer Science
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Research on beam supply control strategy based on sliding mode control
Autorzy:
Zhang, Hao
Dong, Haiying
Zhang, Baoping
Wu, Tong
Chen, Changwen
Powiązania:
https://bibliotekanauki.pl/articles/141014.pdf
Data publikacji:
2020
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
beam supply
DC/DC converter
robustness
sliding mode control
Opis:
In the hybrid multiple H-bridge topology of beam supply, the load change of a DC/DC full-bridge converter can greatly affect the output voltage during onsite operation. An improved sliding mode control (SMC) strategy is thus proposed in this paper, where the rate of switching control is added to the law of system equivalent control to create a law that can realize a complete sliding mode control. Considering the special operating conditions of the load can have an influence on the performance of the controller, the impact of uncertainty existing in onsite conditions is suppressed with the proposed strategy utilized. The validity of the proposed strategy, finally, is verified by simulation, which proves the outperformance of the system in both robustness and dynamics.
Źródło:
Archives of Electrical Engineering; 2020, 69, 2; 349-364
1427-4221
2300-2506
Pojawia się w:
Archives of Electrical Engineering
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
A novel method to calculate the mechanical properties of cancer cells based on atomic force microscopy
Autorzy:
Zhang, T
Zhao, Y.
Tong, Z.
Guang, Y.
Powiązania:
https://bibliotekanauki.pl/articles/307289.pdf
Data publikacji:
2016
Wydawca:
Politechnika Wrocławska. Oficyna Wydawnicza Politechniki Wrocławskiej
Tematy:
właściwości mechaniczne
AFM
moduł Younga
cells’ mechanical properties
eliminating thermal drift
Young's modulus
differential Hertz’s model
Opis:
Purpose: Mechanical properties, as the inherent characteristics of cells, play a critical role in many essential physiological processes, including cell differentiation, migration, and growth. The mechanical properties of cells are one of the criteria that help to determine whether the tissue contains lesions at the single cell level, and it is very important for the early prevention and accurate diagnosis of diseases. Atomic force microscopy (AFM) makes it possible to measure the mechanical properties at single cell level in physiological state. This paper presents a novel method to calculate the mechanical properties of cancer cells more accurately through Atomic force microscopy. Methods: A new induced equation of Hertz’s model, called differential Hertz’s model, has been proposed to calculate the mechanical properties of cancer cells. Moreover, the substrate effect has also been effectively reduced through comparing the calculated mechanical properties of cell at different cell surface areas. Results: The results indicate that the method utilized to calculate the mechanical properties of cells can effectively eliminate the errors in calculation, caused by the thermal drift of AFM system and the substrate effect, and thus improve the calculation accuracy. Conclusion: The mechanical properties calculated by our method in this study are closer to the actual value. Thus, this method shows potential for use in establishing a standard library of Young’s modulus.
Źródło:
Acta of Bioengineering and Biomechanics; 2016, 18, 1; 19-24
1509-409X
2450-6303
Pojawia się w:
Acta of Bioengineering and Biomechanics
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Design and fabrication of 125I seeds for brachytherapy using capillary-based microfluidic technique
Autorzy:
Wang, Yuan
Zhang, Miao
Song, Tong
Chang, Zhenqi
Powiązania:
https://bibliotekanauki.pl/articles/2055673.pdf
Data publikacji:
2021
Wydawca:
Instytut Chemii i Techniki Jądrowej
Tematy:
125I seeds
brachytherapy source
microfluidic
Monte Carlo calculation
core-shell
Opis:
A new kind of 125I seeds with a core-shell structure were synthesized by an easy assembling–disassembling coaxial capillaries microfluidic device. The dose distribution of a 125I brachytherapy source fabricated by arranging six 125I seeds collinearly within a cylindrical titanium capsule was simulated by modelling the source in a water phantom using Monte Carlo N-Particle Transport code. The infl uence of the motion and the core size of the 125I seeds on the dose distribution was also studied in this work.
Źródło:
Nukleonika; 2021, 66, 2; 55--60
0029-5922
1508-5791
Pojawia się w:
Nukleonika
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Influence of grouting pressure volatility on additional response of adjacent pile foundation in shield construction
Autorzy:
Zhang, Chuan-Chuan
Li, Dan-Mei
Zhang, Jun
Yu, Tong-Sheng
Chen, Qiang
Powiązania:
https://bibliotekanauki.pl/articles/2086958.pdf
Data publikacji:
2022
Wydawca:
Polskie Towarzystwo Mechaniki Teoretycznej i Stosowanej
Tematy:
grouting pressure fluctuations
adjacent loaded-pile foundation
normal distribution
FLAC3D
Opis:
Soil parameters along the heading direction are subjected to spatial variability during shield construction, so grouting pressure requires constant adjustment to ensure ground stress sta- bility. This causes grouting pressure to fluctuate around the design pressure/curve. There- fore, the influence of the grouting pressure volatility on the adjacent loaded-pile foundation should be considered in shield tunneling. In this study, a refined numerical simulation of the shield construction process is conducted using the Fast Lagrangian Analysis of Continua in Three Dimensions (FLAC3D) software. A total of 300 groups of grouting pressure pa- rameters with a skewed normal distribution are input into the numerical model. Then, the influence of the construction parameter uncertainty on the adjacent loaded-pile foundation is analyzed. Finally, the back analysis method is conducted based on the monitored data to evaluate how the construction process affects the pile foundation. The calculation results are compared with those of the traditional finite element method. The results indicate that in the tunneling process, the grouting pressure fluctuation greatly affects the additional bend- ing moment of the adjacent pile foundation. Under the influence of the grouting pressure, the additional axial force and additional bending moment of the pile foundation also follow the skewed normal distribution. The back analysis results of the pile foundation are greater than those of the typical numerical method by about 60% 100%, that is using the back analysis calculation results to evaluate the pile foundation additional response can reduce the risk.
Źródło:
Journal of Theoretical and Applied Mechanics; 2022, 60, 1; 33--47
1429-2955
Pojawia się w:
Journal of Theoretical and Applied Mechanics
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
An improved air supply scheme for battery energy storage systems
Autorzy:
Xinlong, Zhu
Hong, Shi
Wenbing, Xu
Tong, Zhang
Yansong, Wang
Powiązania:
https://bibliotekanauki.pl/articles/2173643.pdf
Data publikacji:
2022
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
battery energy storage system
BESS
air cooling duct
baffles
akumulatorowy system magazynowania energii
kanał chłodzący powietrze
przegrody
Opis:
The overall efficiency of battery energy storage systems (BESSs) strongly depends on the temperature uniformity of the batteries, usually disregarded in studies of the integrated performance of BESSs. This paper presents a new battery thermal management system (BTMS) using a personalized air supply instead of a central air supply. Thermal models are established to predict the thermal behavior of BESSs with 400 battery packs. Moreover, several optimizations comprising the effect of the position and number of air inlets, the number, and angle of the baffles on the air distribution in the ducts are proposed. The results show that the distributed air supply from the main air inlet makes the air velocity in the main air ducts more uniform. It is demonstrated that air deflection is the main source of airflow inhomogeneity at the air outlets. The airflow uniformity is better when the baffles are added at the entrance and the bottom of each riser duct than at other locations. The improved air supply scheme makes the nonuniformity coefficient of air velocity reduced from 1.358 to 0.257. The findings can guide the selection of a cooling form to enhance the safety of BESSs.
Źródło:
Bulletin of the Polish Academy of Sciences. Technical Sciences; 2022, 70, 2; art. no. e140692
0239-7528
Pojawia się w:
Bulletin of the Polish Academy of Sciences. Technical Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Effective separation of specularite and aegirite using chitosan as a novel depressant
Autorzy:
Li, Mingyang
Zhang, Pengpeng
Gao, Xiangpeng
Tong, Xiong
Xie, Xian
Powiązania:
https://bibliotekanauki.pl/articles/2175424.pdf
Data publikacji:
2022
Wydawca:
Politechnika Wrocławska. Oficyna Wydawnicza Politechniki Wrocławskiej
Tematy:
flotation
iron-bearing silicates
aegirite
specularite
chitosan
depressant
Opis:
As a typical iron-bearing silicate gangue, aegirite often associates with specularite. Due to the iron element contained in aegirite, it has similar surface properties to specularite. Flotation is by far one of the most efficient methods of processing this kind of iron ore. But the traditional depressants unable to take action in the separation of specularite and aegirite. Chitosan was used as a novel depressant to attempt to separate specularite from aegirite through microflotation tests, adsorption tests, contact angle measurements, Zeta potential measurements, and XPS analysis. The flotation results indicate that chitosan show more strong depression effect on specularite than aegirite. Zeta potential measurements, contact angle measurements and adsorption tests demonstrate that chitosan is more inclined to adsorb on the specularite surface than aegirite, which hinders the subsequent adsorption of collector sodium oleate and increases difference in hydrophobicity between the two minerals. The XPS results of specularite validate the adsorption of chitosan on specularite, and illustrate that electrons of chitosan were partially transferred to oxygen and iron atoms in specularite during the adsorption process.
Źródło:
Physicochemical Problems of Mineral Processing; 2022, 58, 6; art. no. 151692
1643-1049
2084-4735
Pojawia się w:
Physicochemical Problems of Mineral Processing
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Ammonium chloride’s weakening effect on the copper activation of pyrite in flotation and the surface regulation mechanism behind it
Autorzy:
Zhang, Shengdong
Chen, Yumeng
Tong, Xiong
Xie, Xian
Lu, Yalin
Powiązania:
https://bibliotekanauki.pl/articles/110323.pdf
Data publikacji:
2019
Wydawca:
Politechnika Wrocławska. Oficyna Wydawnicza Politechniki Wrocławskiej
Tematy:
pyrite
depression
cuprammonium solution
pH buffering property
flotation
Opis:
The traditional separation process of pyrite and marmatite is carried out under highly alkaline conditions. Therefore, a large amount of lime is demanded and the zinc recovery cannot be guaranteed. However, under weakly alkaline conditions, copper-activated pyrite has good floatability, which is difficult to separate from marmatite. In this paper, ammonium chloride (NH4Cl) is used for depressing the flotation of copper-activated pyrite to achieve the separation of these two minerals under weakly alkaline environment. The flotation tests show that NH4Cl can significantly reduce the floatability of pyrite in weakly alkaline conditions. The results of adsorption tests and X-ray photoelectron spectroscopy (XPS) analyses indicate that NH4Cl can obviously change the composition of pyrite surface by increasing the content of iron/copper hydroxide and reducing the content of copper sulfides. Calculation of the solution composition demonstrates that the addition of NH4Cl results in the occurrence of Cu(NH3)n2+ and the pH buffering property. Based on these results, it can be concluded that the depression of NH4Cl on copper activated pyrite is mainly derived from two aspects: 1) the pH buffering property of the conjugated acid-base pair (NH4+/NH3) can impede the decline of OH- concentration, which results in more hydroxide adsorbed on pyrite; 2) NH3 (aq) competes with the pyrite surface to consume Cu2+through complexation, which causes a reduction in the amount of copper sulfides formed on the pyrite surface.
Źródło:
Physicochemical Problems of Mineral Processing; 2019, 55, 5; 1070-1081
1643-1049
2084-4735
Pojawia się w:
Physicochemical Problems of Mineral Processing
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Comparison of the depression effect of two thiol depressants on the separation of specularite and aegirite
Autorzy:
Li, Mingyang
Zhang, Chen
Gao, Xiangpeng
Xie, Xian
Tong, Xiong
Powiązania:
https://bibliotekanauki.pl/articles/2175426.pdf
Data publikacji:
2022
Wydawca:
Politechnika Wrocławska. Oficyna Wydawnicza Politechniki Wrocławskiej
Tematy:
aegirite
specularite
flotation
depressant
iron-bearing silicate
Opis:
In this work, two thiol-type reagents, thioglycolic acid (TGA) and mercaptopropionic acid (MPA), were firstly exploited and compared as aegirite depressants with sodium oleate (NaOl) as the collector to separate specularite from aegirite by flotation. The adsorption performances and mechanisms of TGA and MPA on aegirite surface were investigated via flotation experiments, Zeta potential tests, adsorption measurements, contact angle dimensions, and surface characterizations. The results of flotation indicated that TGA and MPA exhibited a considerable depression impact on the flotation of aegirite but little effect on specularite. TGA depicted more excellent depression performance than MPA, which was confirmed by HLB calculation. The results demonstrated that TGA and MPA favorably adsorbed on aegirite surface instead of specularite, hindering the subsequent adsorption of NaOl on specularite and resulting in the surface being hydrophilic. XPS results revealed that TGA and MPA were significantly adsorbed on the surface of aegirite through an interaction between the carboxyl and thiol groups of the depressants and the Si and Fe on the surface of aegirite.
Źródło:
Physicochemical Problems of Mineral Processing; 2022, 58, 6; art. no. 152164
1643-1049
2084-4735
Pojawia się w:
Physicochemical Problems of Mineral Processing
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
A mechanism reliability analysis method considering environmental influence and failure modes’ correlation : a case study of rifle automaton
Autorzy:
Fang, Yi-chuan
Wang, Yong-juan
Sha, Jin-long
Gu, Tong-guang
Zhang, He
Powiązania:
https://bibliotekanauki.pl/articles/24200826.pdf
Data publikacji:
2023
Wydawca:
Polska Akademia Nauk. Polskie Naukowo-Techniczne Towarzystwo Eksploatacyjne PAN
Tematy:
mechanism reliability
environmental influence
failure modes’ correlation
copula function
Kaplan-Meier estimation
rifle automaton
Opis:
In order to overcome the challenge of quantifying the influence of environmental conditions and the coexistence of multiple failure modes involved in mechanism reliability modelling under different environments. In this paper, we propose a method for the analysis of mechanism reliability that takes into account the influence of environmental factors and failure modes’ correlation, quantifies the influence of environmental factors as the random distribution and degradation path of parameters, and derives the Copula description of failure mode correlation from the historical data of environmental experiments. On the basis of the discrete mechanism dynamics model, the output parameters of the characteristic points are calculated, and the failure rate of each failure mode is calculated based on the failure criterion and the performance margin theory. Additionally, the dynamic change pattern of the mechanism reliability is compared with the Kaplan-Meier estimation of the corresponding environmental test history data to assess the validity of the calculation results. The reliability modelling problem of a motion mechanism of an automatic rifle automaton in a high and low temperature environment is applied to the method, and the reliability calculation results are close to those of Kaplan-Meier estimation of the test history data, and all are within the upper and lower bounds given by the reliability boundary theory, demonstrating the method's validity.
Źródło:
Eksploatacja i Niezawodność; 2023, 25, 2; art. no. 166145
1507-2711
Pojawia się w:
Eksploatacja i Niezawodność
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Research on communication emitter identification based on semi-supervised dimensionality reduction in complex electromagnetic environment
Autorzy:
Ge, Wei
Qi, Lin
Tong, Lin
Zhu, Jun
Zhang, Jing
Zhao, Dongyang
Li, Ke
Powiązania:
https://bibliotekanauki.pl/articles/27311449.pdf
Data publikacji:
2023
Wydawca:
Polska Akademia Nauk. Czasopisma i Monografie PAN
Tematy:
communication emitter identification
feature extraction
dimensionality reduction
VMD
ESDA
variational mode decomposition
exponential semi-supervised discriminant analysis
identyfikacja emitera komunikacyjnego
ekstrakcja cech
redukcja wymiarowości
rozkład w trybie wariacyjnym
analiza dyskryminacyjna wykładnicza półnadzorowana
Opis:
The individual identification of communication emitters is a process of identifying different emitters based on the radio frequency fingerprint features extracted from the received signals. Due to the inherent non-linearity of the emitter power amplifier, the fingerprints provide distinguishing features for emitter identification. In this study, approximate entropy is introduced into variational mode decomposition, whose features performed in each mode which is decomposed from the reconstructed signal are extracted while the local minimum removal method is used to filter out the noise mode to improve SNR. We proposed a semi-supervised dimensionality reduction method named exponential semi-supervised discriminant analysis in order to reduce the high-dimensional feature vectors of the signals, and LightGBM is applied to build a classifier for communication emitter identification. The experimental results show that the method performs better than the state-of-the-art individual communication emitter identification technology for the steady signal data set of radio stations with the same plant, batch and model.
Źródło:
Bulletin of the Polish Academy of Sciences. Technical Sciences; 2023, 71, 4; art. no. e145766
0239-7528
Pojawia się w:
Bulletin of the Polish Academy of Sciences. Technical Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Transcriptomic landscape of Dendrobium huoshanense and its genes related to polysaccharide biosynthesis
Autorzy:
Han, R.
Xie, D.
Tong, X.
Zhang, W.
Liu, G.
Peng, D.
Yu, N.
Powiązania:
https://bibliotekanauki.pl/articles/57911.pdf
Data publikacji:
2018
Wydawca:
Polskie Towarzystwo Botaniczne
Opis:
Dendrobium huoshanense has long been used to treat various diseases in oriental medicine. In order to study its gene expression profile, transcripts involved in the biosynthesis of precursors of polysaccharides, as well as mechanisms underlining morphological differences between wild and cultivated plants, three organs of both wild type and cultivated D. huoshanense were collected and sequenced by Illumina HiSeq4000 platform, yielding 919,409,540 raw reads in FASTQ format. After Trinity de novo assembly and quality control, 241,242 nonredundant contigs with the average length of 967.5 bp were generated. qRT-PCR experiment on the selected transcripts showed the transcriptomic data were reliable. BLASTx was conducted against NR, SwissProt, String, Pfam, and KEGG. Gene ontology annotation revealed more than 40,000 contigs assigned to catalytic activity and metabolic process, suggesting its dynamic physiological activities. By searching KEGG pathway, six genes potentially involved in mannose biosynthetic pathway were retrieved. Gene expression analysis for stems between wild and cultivated D. huoshanense resulted in 956 genes differentially expressed. Simple sequence repeats (SSRs) analysis revealed 143 SSRs with the unit size of 4 and 3,437 SSRs the size of 3. The obtained SSRs are the potential molecular markers for discriminating distinct cultivars of D. huoshanense.
Źródło:
Acta Societatis Botanicorum Poloniae; 2018, 87, 1
0001-6977
2083-9480
Pojawia się w:
Acta Societatis Botanicorum Poloniae
Dostawca treści:
Biblioteka Nauki
Artykuł
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