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Tytuł:
Indirect enzyme-linked immunosorbent assay method based on Streptococcus agalactiae rSip-Pgk-FbsA fusion protein for detection of bovine mastitis
Autorzy:
Bu, R.-E.
Wang, I.-L.
Wu, J.-H.
Xilin, G.W.
Chen, J.-L.
Wang, H.
Powiązania:
https://bibliotekanauki.pl/articles/30156.pdf
Data publikacji:
2017
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
Objective: The aim of this study was to establish a rapid and accurate method for the detection of the Streptococcus agalactiae antibody (SA-Ab) to determine the presence of the bovine mastitis (BM)-causative pathogen. Methods: The multi-subunit fusion protein rSip-Pgk-FbsA was prokaryotically expressed and purified. The triple activities of the membrane surface-associated proteins Sip, phosphoglycerate kinase (Pgk), and fibronectin (FbsA) were used as the diagnostic antigens to establish an indirect enzyme-linked immunosorbent assay (ELISA) method for the detection of SA-Ab in BM. Results: The optimal antigen coating concentration was 2 μg/mL, the optimal serum dilution was 1:160, and the optimal dilution of the enzyme-labeled secondary antibody was 1:6000. The sensitivity, specificity, and repeatability tests showed that the method established in this study had no cross-reaction with antibodies to Streptococcus pyogenes, Escherichia coli, Staphylococcus aureus, and Staphylococcus epidermidis in the sera. The results of the sensitivity test showed that a positive result could be obtained even if the serum dilution reached 1:12,800, indicating the high sensitivity and good repeatability of the method. The positive coincidence rate of this method was 98.6%, which is higher than that of previous tests established with the Sip or Pgk mono-antigen fusion protein, respectively, demonstrating the relatively higher sensitivity of this newly established method. The detection rate for 389 clinical samples was 46.53%. Conclusions: The indirect ELISA method established in this study could provide a more accurate and reliable serological method for the rapid detection of S. agalactiae in cases of BM.
Źródło:
Polish Journal of Veterinary Sciences; 2017, 20, 2
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
A Lifting Wavelet Domain Audio Watermarking Algorithm Based on the Statistical Characteristics of Sub-Band Coefficients
Autorzy:
Tao, Z.
Zhao, H.
Wu, J.
Gu, J.
Xu, Y.
Wu, D.
Powiązania:
https://bibliotekanauki.pl/articles/177956.pdf
Data publikacji:
2010
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
audio watermarking
lifting wavelet transform
statistical characteristics
sub-band coefficients
Opis:
In this paper, a new lifting wavelet domain audio watermarking algorithm based on the statistical characteristics of sub-band coefficients is proposed. First of all, an original audio signal was segmented and each segment was divided into two sections. Then, the Barker code was used for synchronization, the LWT (lifting wavelet transform) was performed on each section, a synchronization code and a watermark were embedded into the first section and the second section, respectively, by modifying the statistical average value of the sub-band coefficients. The embed strength was determined adaptively according to the auditory masking property. Experiments show that the embedded watermark has better robustness against common signal processing attacks than present algorithms based on LWT and can resist random cropping in particular.
Źródło:
Archives of Acoustics; 2010, 35, 4; 481-491
0137-5075
Pojawia się w:
Archives of Acoustics
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The effects of cavity geometry on an aspirated compressor cascade
Autorzy:
Guo, S.
Lu, H.-W.
Liu, J.
Wu, C.-J.
Powiązania:
https://bibliotekanauki.pl/articles/280871.pdf
Data publikacji:
2014
Wydawca:
Polskie Towarzystwo Mechaniki Teoretycznej i Stosowanej
Tematy:
highly loaded compressor cascade
boundary layer suction
suction flow distribution
off-take cavity
Opis:
The impact of the bleed off-take cavity within aspirated blades on the aerodynamic perfor- mance of a highly loaded compressor cascade was explored. Three-dimensional Navier-Stokes simulations were conducted in rectangular compressor cascades after validation against expe- rimental results were done. Five different off-take configurations with varying suction loca- tion were numerically evaluated. The results show that the regularities of the suction flow distribution on the suction slot inlet greatly influence the benefits of boundary layer suction. The distribution style greatly depends not only on the flow condition of the blade passage, but also on the realization of the suction process and the geometry of the cavity inside the aspirated blade. The low kinetic fluid in the endwall region compared with that around the midspan is usually prone to be sucked into the suction slot. The distribution of the suc- tion mass flow along the spanwise direction would be symmetrical if the suction position is properly selected and the inside off-take cavity is well designed, even though the bleeding system has been simplified to be asymmetrical as employed in the present document.
Źródło:
Journal of Theoretical and Applied Mechanics; 2014, 52, 1; 117-128
1429-2955
Pojawia się w:
Journal of Theoretical and Applied Mechanics
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Training safely, Training safety
Autorzy:
Wu, J.
An, M.
Jin, Y.
Geng, H.
Powiązania:
https://bibliotekanauki.pl/articles/116104.pdf
Data publikacji:
2014
Wydawca:
Uniwersytet Morski w Gdyni. Wydział Nawigacyjny
Tematy:
safety at sea
Training of Crew
Maritime Education and Training (MET)
Training Safety
Maritime Safety Education
Marine Survival
SOLAS Convention
Self Protection
Opis:
It is the basic requirement of maritime safety education to guarantee the safety of teaching operation while training the crew's occupation safety capability. Marine Training Center of Shanghai Maritime University has undertaken the practical teaching of "marine survival" for many years and come up with the whole safety procedures of training. Based on the requirements of SOLAS convention and regulations of STCW over crew training, this paper introduces the safety allocation, utilization and maintenance of teaching equipments. Through the investigation of the safety situation of students' practical operation, the safety teaching method named "four in one" has been put forward, which includes the pre-teaching safety precaution, the whole monitor during the teaching process, the post-teaching summary evaluation, and the reset and standby of teaching facilities. Finally, during the learning and training of "marine survival", crews and students are called on to place priority on personal safety rather than acquisition of knowledge and skills. Only in this way can they be capable of self-protection and protection of others in the career of seafaring.
Źródło:
TransNav : International Journal on Marine Navigation and Safety of Sea Transportation; 2014, 8, 3; 423-427
2083-6473
2083-6481
Pojawia się w:
TransNav : International Journal on Marine Navigation and Safety of Sea Transportation
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Speech Enhancement Based on the Multi-Scales and Multi-Thresholds of the Auditory Perception Wavelet Transform
Autorzy:
Tao, Z.
Zhao, H. M.
Zhang, X-J.
Wu, D.
Powiązania:
https://bibliotekanauki.pl/articles/177021.pdf
Data publikacji:
2011
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
speech enhancement
low SNR
auditory perception wavelet transform
unvoiced enhancement
masking effect
Opis:
This paper proposes a speech enhancement method using the multi-scales and multi-thresholds of the auditory perception wavelet transform, which is suitable for a low SNR (signal to noise ratio) environment. This method achieves the goal of noise reduction according to the threshold processing of the human ear’s auditory masking effect on the auditory perception wavelet transform parameters of a speech signal. At the same time, in order to prevent high frequency loss during the process of noise suppression, we first make a voicing decision based on the speech signals. Afterwards, we process the unvoiced sound segment and the voiced sound segment according to the different thresholds and different judgments. Lastly, we perform objective and subjective tests on the enhanced speech. The results show that, compared to other spectral subtractions, our method keeps the components of unvoiced sound intact, while it suppresses the residual noise and the background noise. Thus, the enhanced speech has better clarity and intelligibility.
Źródło:
Archives of Acoustics; 2011, 36, 3; 519-532
0137-5075
Pojawia się w:
Archives of Acoustics
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Eddy current density asymmetric distribution of damper bars in bulb tubular turbine generator
Autorzy:
Qiu, H.
Fan, X.
Yi, R.
Feng, J.
Wu, J.
YANG, C.
ZHAO, H.
Powiązania:
https://bibliotekanauki.pl/articles/140467.pdf
Data publikacji:
2017
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
damper bars
bulb tubular turbine generator
electromagnetic field
eddy current
Opis:
The major reasons that cause the damage of damper bars in the leeward side are found in this paper. It provides a route for the structure optimization design of a hydro generator. Firstly, capacity of a 24 MW bulb tubular turbine generator is taken as an example in this paper. The transient electromagnetic field model is established, and the correctness of the model is verified by the comparison of experimental results and simulation data. Secondly, when the generator is operated at rated condition, the eddy current density distributions of damper bars are studied. And the asymmetric phenomenon of the eddy current density on damper bars is discovered. The change laws of the eddy currents in damper bars are determined through further analysis. Thirdly, through the study of eddy current distributions under different conditions, it is confirmed that the stator slots and armature reaction are the main factors to affect the asymmetric distribution of the eddy current in damper bars. Finally, the studies of the magnetic density distribution and theoretical analysis revealed the asymmetric distribution mechanism of eddy current density.
Źródło:
Archives of Electrical Engineering; 2017, 66, 3; 571-581
1427-4221
2300-2506
Pojawia się w:
Archives of Electrical Engineering
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Advanced Characterization of Material Properties on the Nanometer Scale Using Atomic Force Microscopy
Autorzy:
Fenner, M.
Wu, S.
Yu, J.
Huber, H.
Kienberger, F.
Powiązania:
https://bibliotekanauki.pl/articles/1490089.pdf
Data publikacji:
2012-02
Wydawca:
Polska Akademia Nauk. Instytut Fizyki PAN
Tematy:
77.55.-g
07.79.-v
68.37.Ps
68.37.Uv
07.57.Pt
Opis:
We report recent advances in material characterization on the nanometer scale using scanning microwave microscopy. This combines atomic force microscopy and a vector network analyzer using microwave tip sample interaction to characterize dielectric and electronic material properties on the nanometer scale. We present the methods for calibration as well as applications. Scanning microwave microscopy features calibrated measurements of: (1) capacitance with attofarad sensitivity. For calibration a well characterized array of capacitors (0.1 fF to 10 fF) is used. The method is applied to determine the dielectric properties of thin organic films, (2) Semiconductor dopant density. Calibration is performed by imaging the cross-section of a standard sample with differently doped layers (dopant stair case) from $10^{16}$ atoms/$cm^3$ to $10^{20}$ atoms/$cm^3$.
Źródło:
Acta Physica Polonica A; 2012, 121, 2; 416-419
0587-4246
1898-794X
Pojawia się w:
Acta Physica Polonica A
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
A fault diagnosis method for substation grounding grid based on the square-wave frequency domain model
Autorzy:
Zhang, P.-H.
He, J.-J.
Zhang, D.-D.
Wu, L.-M.
Powiązania:
https://bibliotekanauki.pl/articles/221635.pdf
Data publikacji:
2012
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
square-wave
frequency characteristics
grounding grid
fault diagnosis
Opis:
Current methods of fault diagnosis for the grounding grid using DC or AC are limited in accuracy and cannot be used to identify the locations of the faults. In this study, a new method of fault diagnosis for substation grounding grids is proposed using a square-wave. A frequency model of the grounding system is constructed by analyzing the frequency characteristics of the soil and the grounding conductors into which two different frequency square-wave sources are injected. By analyzing and comparing the corresponding information of the surface potentials of the output signals, the faults of the grounding grid can be diagnosed and located. Our method is verified by software simulation, scale model experiments and field experiments.
Źródło:
Metrology and Measurement Systems; 2012, 19, 1; 63-71
0860-8229
Pojawia się w:
Metrology and Measurement Systems
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
pUC18-CpG stimulates RAW 264.7 via TBK1-mediated pathway and presents adjuvanticity in mice
Autorzy:
Wu, J.
Chen, Q.
Xin, T.
Sun, Y.
Jia, H.
Hou, S.H.
Guo, X.Y.
Powiązania:
https://bibliotekanauki.pl/articles/2087554.pdf
Data publikacji:
2019
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
adjuvant
CpG DNA
Echinococcus granulosus
TBK1
type I interferons
Źródło:
Polish Journal of Veterinary Sciences; 2019, 2; 195-201
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Saving water used for vegetable production by applying regulated deficit irrigation practices
Autorzy:
Chang, Y.-S.
Lin, K.-H.
Chen, Y.-J.
Wu, C.-W.
Chang, Y.-J.
Powiązania:
https://bibliotekanauki.pl/articles/13003875.pdf
Data publikacji:
2021
Wydawca:
Uniwersytet Przyrodniczy w Lublinie. Wydawnictwo Uniwersytetu Przyrodniczego w Lublinie
Źródło:
Acta Scientiarum Polonorum. Hortorum Cultus; 2021, 20, 3; 27-36
1644-0692
Pojawia się w:
Acta Scientiarum Polonorum. Hortorum Cultus
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Magnified holographic projection based on spatial light modulators
Autorzy:
Su, Y.
Cai, Z.
Shi, L.
Zhou, F.
Chen, H.
Wu, J.
Powiązania:
https://bibliotekanauki.pl/articles/174247.pdf
Data publikacji:
2018
Wydawca:
Politechnika Wrocławska. Oficyna Wydawnicza Politechniki Wrocławskiej
Tematy:
holographic display
spatial light modulators
computer-generated holograms
magnified holographic projection
Opis:
In this paper, a magnified holographic projection based on spatial light modulators is proposed and implemented by combining four magnification methods, including similarity principle of Fourier transform, spatial division, digital lens, and image splicing methods. The Fourier holographic display system is constructed for the experimental verification of the proposed methods. With such four methods of holographic magnification, the reconstruction image can be magnified to 10 × 5 times in two-dimensional directions, which is verified by the experiments. Furthermore, the undesirable light of holographic projection is eliminated by encoding the linear phase onto the computer-generated holograms. The experimental results prove that the proposed system can realize magnified holographic projection with good reconstructed quality, which provides a promising potential for the dynamic holographic projector.
Źródło:
Optica Applicata; 2018, 48, 4; 589-600
0078-5466
1899-7015
Pojawia się w:
Optica Applicata
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
A polarization selective beam splitter based on a subwavelength multisubpart profile grating structure
Autorzy:
Xiao, Y.
Huang, L.
Wu, H.
Sun, X.
Xiao, W.
Duan, J.
Powiązania:
https://bibliotekanauki.pl/articles/174760.pdf
Data publikacji:
2016
Wydawca:
Politechnika Wrocławska. Oficyna Wydawnicza Politechniki Wrocławskiej
Tematy:
diffraction and gratings
polarizing beam splitter
PBS
leaky-mode resonance
LMR
Opis:
In this study, a polarization selective beam splitter constructed by only a single layer subwavelength multisubpart profile grating is presented. Rigorous coupled-wave analysis is adopted to investigate the properties of the structure. It is shown that for a transverse electric polarized wave, the device demonstrates very high reflectivity (>97%) from 1.46 to 1.58 μm; and for a transverse magnetic polarized wave, at the wavelength of 1.55 μm, it exhibits about 50/50 beam ratio under normal incidence. To evaluate the response of the polarizing beam splitters under variation in structure parameters, we also investigated the fabrication tolerances of the device.
Źródło:
Optica Applicata; 2016, 46, 4; 665-672
0078-5466
1899-7015
Pojawia się w:
Optica Applicata
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Flotation behaviour and surface characteristic of anosovite in a sodium oleate solution
Autorzy:
Wang, Y.
Wen, S.
Zhang, J.
Wu, D.
Xian, Y.
Shen, H.
Powiązania:
https://bibliotekanauki.pl/articles/110766.pdf
Data publikacji:
2017
Wydawca:
Politechnika Wrocławska. Oficyna Wydawnicza Politechniki Wrocławskiej
Tematy:
anosovite
sodium oleate
flotation
adsorption
Opis:
Properties of anosovite in titanium slag, anosovite flotation response in a collector solution of sodium oleate, and adsorption behaviour of sodium oleate on the mineral surface were studied in the present work using XRD, Raman spectra, flotation tests, zeta potential analysis, FTIR and XPS. The results show that the anosovite crystal contains magnesium, and its chemical composition is Mg0.09Ti2.91O5. The chemical bonds on the anosovite surface mainly comprise Ti-O bonds. Micro-flotation tests indicate that anosovite has better floatability at a wide pH range and the recovery reaches 88% at pH=6, when the dosage of sodium oleate is only 4·10−6 mol/dm3. The point of zero charge of anosovite was determined near pH 3.2 by the zeta potential measurement. In the flotation process, chemical adsorption occurs between the carboxyl of sodium oleate and the titanium sites on the anosovite surface.
Źródło:
Physicochemical Problems of Mineral Processing; 2017, 53, 2; 714-723
1643-1049
2084-4735
Pojawia się w:
Physicochemical Problems of Mineral Processing
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Demonstration of a new indicator for studying upwelling in the Northern South China Sea
Autorzy:
Lin, L.
Wang, Y.-S.
Sun, C.-C.
Li, N.
Wang, H.
Mitchell, B.G.
Wu, M.-L.
Song, H.
Wu, J.-F.
Powiązania:
https://bibliotekanauki.pl/articles/48889.pdf
Data publikacji:
2011
Wydawca:
Polska Akademia Nauk. Instytut Oceanologii PAN
Tematy:
China Sea
cluster analysis
multivariate statistical analysis
principal component analysis
remote sensing
satellite monitoring
sea surface temperature
silicate
spatial distribution
upwelling
Opis:
In order to demonstrate that silicate (SiO3-Si) can be used as an indicator to study upwelling in the northern South China Sea, hierarchical cluster analysis (CA) and principle component analysis (PCA) were applied to analyse the metrics of the data consisting of 14 physical-chemical-biological parameters at 32 stations. CA categorized the 32 stations into two groups (low and high nutrient groups). PCA was applied to identify five Principal Components (PCs) explaining 78.65% of the total variance of the original data. PCA found important factors that can describe nutrient sources in estuarine, upwelling, and non-upwelling areas. PC4, representing the upwelling source, is strongly correlated to SiO3-Si. The spatial distribution of silicate from the surface to 200 m depth clearly showed the upwelling regions, which is also supported by satellite observations of sea surface temperature.
Źródło:
Oceanologia; 2011, 53, 2
0078-3234
Pojawia się w:
Oceanologia
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Signature of Hot Phonons in Reliability of Nitride Transistors and Signal Delay
Autorzy:
Matulionis, A.
Liberis, J.
Matulionienė, I.
Šermukšnis, E.
Leach, J.
Wu, M.
Ni, X.
Morkoç, H.
Powiązania:
https://bibliotekanauki.pl/articles/1506173.pdf
Data publikacji:
2011-02
Wydawca:
Polska Akademia Nauk. Instytut Fizyki PAN
Tematy:
72.70.+m
73.50.Fq
73.61.Ey
Opis:
Lifetime of non-equilibrium (hot) phonons in biased GaN heterostructures with two-dimensional electron gas channels was estimated from hot-electron fluctuations. Dependence of the lifetime on the electron density is not monotonous - the resonance-type fastest decay serves as a signature of hot phonons. The signature is resolved in nitride heterostructure field effect transistors when the gate voltage is used to change the channel electron density. The transistor cut-off frequency decreases on both sides of the resonance in agreement with the enhanced electron scattering caused by longer hot-phonon lifetimes. The signature is also noted in device reliability experiment: the enhanced temperature of hot phonons, possibly, triggers formation of new defects and accelerates device degradation.
Źródło:
Acta Physica Polonica A; 2011, 119, 2; 225-227
0587-4246
1898-794X
Pojawia się w:
Acta Physica Polonica A
Dostawca treści:
Biblioteka Nauki
Artykuł
Wyświetlanie 1-15 z 24

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