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    Wyświetlanie 1-13 z 13
    Tytuł:
    Amino acid substitutions of His296 alter the catalytic properties of Zymomonas mobilis 10232 levansucrase
    Autorzy:
    Li, Shu
    Chen, Ming
    Li, Gang
    Yan, Yong
    Yu, Hai
    Zhan, Yu
    Peng, Zi
    Wang, Jin
    Lin, Min
    Powiązania:
    https://bibliotekanauki.pl/articles/1040841.pdf
    Data publikacji:
    2008
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    Zymomonas mobilis
    His296
    levansucrase
    levan
    transfructosylation activity
    hydrolysis activity
    Opis:
    His296 of Zymomonas mobilis levansucrase (EC 2.4.1.10) is crucial for the catalysis of the transfructosylation reaction. The three-dimensional structures of levansucrases revealed the His296 is involved in the substrate recognition and binding. In this study, nine mutants were created by site-directed mutagenesis, in which His296 was substituted with amino acids of different polarity, charge and length. The substitutions of His296 with Arg or Trp retained partial hydrolysis and transfructosylation activities. The positively charged Lys substitution resulted in a 2.5-fold increase of sucrose hydrolysis. Substitutions with short (Cys or Ser), negatively charged (Glu) or polar (Tyr) amino acids virtually abolished both the activities. Analysis of transfructosylation products indicated that the mutants synthesized different oligosaccharides, suggesting that amino acid substitutions of His296 strongly affected both the enzyme activity and transfructosylation products.
    Źródło:
    Acta Biochimica Polonica; 2008, 55, 1; 201-206
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Molecular cloning, expression and characterization of Bmserpin-2 gene from Bombyx mori
    Autorzy:
    Pan, Ye
    Xia, Hengchuan
    Lü, Peng
    Chen, KePing
    Yao, Qin
    Chen, Huiqin
    Gao, Lu
    He, Yuanqing
    Wang, Lin
    Powiązania:
    https://bibliotekanauki.pl/articles/1040486.pdf
    Data publikacji:
    2009
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    serpin-2
    Bombyx mori
    bioinformatics
    subcellular location
    qPCR
    Opis:
    Serpins are a broadly distributed family of protease inhibitors. In this study, the gene encoding Bombyx mori serpin-2 (Bmserpin-2) was cloned and expressed in E. coli. The Bmserpin-2 cDNA contains a 1125 bp open reading frame (ORF). The deduced protein has 374 amino-acid residues, contains a conserved SERPIN domain and shares extensive homology with other invertebrate serpins. RT-PCR analysis showed that Bmserpin-2 was expressed in all developmental stages of B. mori larvae and various larval tissues. Subcellular localization analysis indicated that Bmserpin-2 protein was located in the cytoplasm. Interestingly, real-time quantitative PCR revealed that the expression of Bmserpin-2 in the midgut of susceptible B. mori strain 306 significantly increased at 72 hours post inoculation (hpi) when infected with BmNPV. However, there was no significant increase of the Bmserpin-2 expression in resistant strain NB infected with BmNPV. Thus, our data indicates that Bmserpin-2 may be involved in B. mori antiviral response.
    Źródło:
    Acta Biochimica Polonica; 2009, 56, 4; 671-677
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Dft investigation of a high energy density polynitro compound, 2,2’-Bis(trinitromethyl)-5,5’-azo-1,2,3,4- tetrazole
    Autorzy:
    Lin, H.
    Zhu, S. G.
    Chen, P. Y.
    Li, K.
    Li, H. Z.
    Peng, X. H.
    Powiązania:
    https://bibliotekanauki.pl/articles/358030.pdf
    Data publikacji:
    2013
    Wydawca:
    Sieć Badawcza Łukasiewicz - Instytut Przemysłu Organicznego
    Tematy:
    polynitro
    electronic structure
    thermodynamic properties
    crystal structure
    detonation performance
    stability
    Opis:
    A novel polynitro compound, 2,2’-bis(trinitromethyl)-5,5’-azo- 1,2,3,4-tetrazole, was designed and investigated at the DFT-B3LYP/6-31G(d) level. Its properties, such as electronic structure, IR spectrum, heat of formation, thermodynamic properties and crystal structure, were predicted. This compound is most likely to crystallize in the P21 space group, and the corresponding cell parameters are Z = 2, a = 5.46 Å, b = 9.72 Å, c = 14.05 Å, α = 90°, β = 90°, γ = 90°. In addition, the detonation velocity and pressure were also estimated by using the empirical Kamlet-Jacobs equations, and were predicted to be 8.28 km/s and 31.61 GPa respectively. The oxygen balance of this compound is +13.79%, which indicates that it could serve as an oxidizer. Bond dissociation energy calculations show that the C(13)-N(21)O2 and C(14)-N(30)O2 bonds are the locations of thermal decomposition and that this compounds meets the thermal stability requirements as an exploitable explosive. Keywords: polynitro, electronic structure, thermodynamic properties, crystal structure, detonation performance, stability.
    Źródło:
    Central European Journal of Energetic Materials; 2013, 10, 3; 325-338
    1733-7178
    Pojawia się w:
    Central European Journal of Energetic Materials
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    A multiplex PCR for simultaneous detection of classical swine fever virus, African swine fever virus, highly pathogenic porcine reproductive and respiratory syndrome virus, porcine reproductive and respiratory syndrome virus and pseudorabies in swines
    Autorzy:
    Hu, L.
    Lin, X.Y.
    Yang, Z.X.
    Yao, X.P.
    Li, G.L.
    Peng, S.Z.
    Wang, Y.
    Powiązania:
    https://bibliotekanauki.pl/articles/30249.pdf
    Data publikacji:
    2015
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Opis:
    In this assay, we developed and evaluated a multiplex PCR (mPCR) for its ability in detecting multiple infections of swine simultaneously. Four pairs of primers were used to detect five viruses. Specific primers were designed for classical swine fever virus (CSFV), African swine fever virus (ASFV) and pseudorabies (PRV). A pair of primers was designed prudently for two different types of porcine reproductive and respiratory syndrome virus that respectively were porcine reproductive and respiratory syndrome virus (PRRSV), highly pathogenic porcine reproductive and respiratory syndrome virus (HP-PRRSV). The detection limits of the mPCR were 1.09×10⁴, 1.50×10³, 2.10×10³, 1.30×10³ and 8.97×10² copies/reaction for CSFV, ASFV, HP-PRRSV, PRRSV and PRV, respectively. A total of 49 clinical specimens were tested by the mPCR, and the result showed that co-infection by two or three viruses was 51%. In conclusion, the PCR is a useful tool for clinical diagnosis of not only single infections but also mixed infections in swines.
    Źródło:
    Polish Journal of Veterinary Sciences; 2015, 18, 4
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    First-principles calculations on phase transition and elastic properties of CoN
    Autorzy:
    De-Chun, He
    Yong, Peng
    Su-Yuan, Li
    Yong-Lin, He
    Powiązania:
    https://bibliotekanauki.pl/articles/1075580.pdf
    Data publikacji:
    2016-09
    Wydawca:
    Polska Akademia Nauk. Instytut Fizyki PAN
    Tematy:
    62.20.-x
    62.20.de
    62.50.-p
    62.20.dq
    Opis:
    The structural phase transition and elastic properties of CoN are investigated by ab initio plane-wave pseudopotential density function theory method. The equilibrium lattice parameters a₀, elastic constants C_{ij}, bulk modulus B₀ and its derivative B'₀ are calculated. From the usual condition of equal enthalpy, the phase transition of CoN from zinc-blende to rocksalt structure occurs at 35.4 GPa with a volume collapse of about 15.6%, consistent with the calculated result 36 GPa (FP-LDA), but an uncertainty is about 4.4 GPa compared with the 31 GPa (ASA-GGA). All three independent elastic constants, C₁₁, C₁₂, and C₄₄ for CoN are calculated from direct computation of stresses generated by small strains. Both C₁₂ and C₄₄ are less sensitive to pressure as compared with C₁₁. The calculated conclusions offer theoretical data for the further research of the mechanical properties for CoN.
    Źródło:
    Acta Physica Polonica A; 2016, 130, 3; 743-747
    0587-4246
    1898-794X
    Pojawia się w:
    Acta Physica Polonica A
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Prokaryotic expression, purification and antigenicity analysis of African swine fever virus pK205R protein
    Autorzy:
    Wu, X.
    Xiao, L.
    Peng, B.
    Wang, Y.
    Yang, Z.
    Yao, X.
    Hu, L.
    Lin, X.
    Powiązania:
    https://bibliotekanauki.pl/articles/30963.pdf
    Data publikacji:
    2016
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Opis:
    African swine fever is an acute, febrile and highly virulent porcine disease causing serious economic losses worldwide. The pK205R protein of the African swine fever virus (ASFV) is largely expressed in the early stages of infection, which has given the K205R gene extensive attention. In this study, the ASFV K205R was cloned and expressed in Escherichia coli BL21 (DE3). Expression of histidine-tagged pK205R with a molecular mass of 44 kDa was determined by 12% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and Western blot analysis. Optimisation of culture conditions allowed induction of the recombinant protein with 0.4 mM Isopropyl β-D-thiogalactoside (IPTG) at 37oC for 2 h. The protein existed in cellular supernatant and was purified using a Ni-NTA resin column. The purified protein was used to immunize rabbits four times to enable the production of polyclonal antibodies, and the antiserum titre was detected by ELISA. The results showed that the purified pK205R can react with ASFV positive serum specifically by Western blotting. The pK205R had high antigenicity, which indicated that pK205R could be used as an antigen for detection of ASFV-specific antibodies in ELISA testing, and the recombinant protein could contribute to further research of the action and structure of pK205R.
    Źródło:
    Polish Journal of Veterinary Sciences; 2016, 19, 1
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    The distribution of different virulence grass carp reovirus strains in some neglected tissues
    Autorzy:
    Liang, H.R.
    Fu, X.Z.
    Li, N.Q.
    Liu, L.H.
    Lin, Q.
    Li, Y.G.
    Peng, Y.A.
    Huang, Z.B.
    Wu, S.Q.
    Powiązania:
    https://bibliotekanauki.pl/articles/30668.pdf
    Data publikacji:
    2016
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Źródło:
    Polish Journal of Veterinary Sciences; 2016, 19, 4
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Finite Element Treatment of Vortex States in 3D Mesoscopic Cylindrical Superconductors in a Tilted Magnetic Field
    Autorzy:
    Peng, Lin
    Hu, Yahua
    Li, Zhijian
    Deng, Ke
    Zhu, Yanyan
    Xu, Li
    Zhou, Yun
    Powiązania:
    https://bibliotekanauki.pl/articles/1030584.pdf
    Data publikacji:
    2018-01
    Wydawca:
    Polska Akademia Nauk. Instytut Fizyki PAN
    Tematy:
    three-dimensional mesoscopic cylinders
    TDGL equations
    finite element method
    vortex states
    Opis:
    The time-dependent Ginzburg-Landau equations have been solved numerically by a finite element analysis for the mesoscopic superconducting samples with cylindrical shape in a uniform axial magnetic field. We obtain the different vortex patterns as a function of the applied field perpendicular to its surface. We find that multi-vortex states are ground state in three-dimensional mesoscopic cylinders. These results show that our approach is an effective and useful to interpret experimental data on vortex states in the mesoscopic superconductors.
    Źródło:
    Acta Physica Polonica A; 2018, 133, 1; 152-156
    0587-4246
    1898-794X
    Pojawia się w:
    Acta Physica Polonica A
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Comparative Study of 2060-T8 Al-Li Alloy Friction Stir Welded Joints Between Natural Cooling and Water Cooling
    Autorzy:
    Ma, Lin
    Niu, Shiyu
    Ji, Shude
    Gong, Peng
    Powiązania:
    https://bibliotekanauki.pl/articles/355846.pdf
    Data publikacji:
    2020
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    2060-T8 Al-Li alloy
    friction stir welding
    water cooling
    microstructure
    mechanical properties
    Opis:
    2060-T8 Al-Li alloy was friction stir butt welded under natural and water cooling conditions. Microstructures and mechanical properties of the welding joints were mainly compared and discussed. By spraying water on the top surface of stir zone, the grain size was reduced, attributing to the improvement of microhardness. The maximum tensile strength under the water cooling reached 461.1 MPa. The joint fractured at the stir zone due to the thickness reduction and the joint softening. The fracture surface consisted of many dimples with various sizes, indicating the typical ductile fracture. The strategy to apply the low heat input at the welding stage and high cooling rate at the cooling stage during FSW is necessary to obtain a high-quality FSW joint.
    Źródło:
    Archives of Metallurgy and Materials; 2020, 65, 1; 305-312
    1733-3490
    Pojawia się w:
    Archives of Metallurgy and Materials
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Experimental study of dam-break-like tsunami bore impact mechanism on a container model
    Autorzy:
    Chen, Cheng
    Chen, Jun
    Lin, Peng
    Chen, Chiwei
    Chen, Haozhe
    Powiązania:
    https://bibliotekanauki.pl/articles/260608.pdf
    Data publikacji:
    2020
    Wydawca:
    Politechnika Gdańska. Wydział Inżynierii Mechanicznej i Okrętownictwa
    Tematy:
    tsunami bore
    dam break
    container
    Froude number
    movement distance
    coast slope
    Opis:
    Tsunami disasters have frequently occurred in recent years. More and more researchers are focusing on this topic. To investi-gate the tsunami bore impact mechanism on a container model, a multi-functional slope-changing tsunami flume is built in this study. To simulate a tsunami bore, a dam-break wave was generated by a free-falling gate in a reservoir. A needle water level gauge and a high-speed camera were used to measure the tsunami wave heights and velocities for different storage water levels in the test flume, and the corresponding Froude numbers of tsunami waves were also calculated. The factors af-fecting the movement distance of the tsunami wave impacting the container model are explored in this experiment, and the results show that the movement distance is positively correlated with the storage water level, and negatively correlated with the container density and the coast slope.
    Źródło:
    Polish Maritime Research; 2020, 1; 53-59
    1233-2585
    Pojawia się w:
    Polish Maritime Research
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Research on the Acoustic Scattering Characteristics of Multi-Sections Conning Tower Geometries in Monostatic Configuration
    Autorzy:
    Han, Lin-Jiang
    Song, Hao
    Chen, Chang-Xiong
    Peng, Xi-Rui
    Peng, Zi-long
    Powiązania:
    https://bibliotekanauki.pl/articles/2141632.pdf
    Data publikacji:
    2022
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    conning tower
    Kirchhoff approximation
    target strength
    monostatic configuration
    Opis:
    The overall acoustic echo of a submarine is greatly dependent on the conning tower. For enhancing the acoustic stealth performance of a submarine, it is necessary to research an innovative design scheme of the conning tower to reduce its target strength (TS). The aim of this work is to reduce the TS of a conning tower by varying its geometry and streamlining. The accuracy in modelling the acoustic scattering of a conning tower using the Kirchhoff approximation (KA) was validated, compared with finite element analysis (FEA). Several angular conning tower geometries were designed to analyze the effect of streamlining and the number of lateral facets on TS using the KA method. In consideration of the actual situation, the acoustic effect of backing medium was analyzed by compared water-filled elastic hulls with rigid hulls. From the observed TS calculation results, it is shown that the non-streamlined four lateral-facet conning tower geometries are optimal for acoustic stealth performance during the range of incidence angles from −10° to 10°, whereas the streamlined versions have better performance at incidence angles beyond this range. Furthermore, elastic hulls and rigid hulls provide similar spatial distribution regularities in monostatic configuration with the rigidity affecting the magnitude of the TS.
    Źródło:
    Archives of Acoustics; 2022, 47, 3; 307-317
    0137-5075
    Pojawia się w:
    Archives of Acoustics
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Study on Tensile Properties and Microstructures of Different Sites in Al-Si Alloy Casting Component
    Autorzy:
    Tao, Jianquan
    Xiang, Lin
    Chen, Xidong
    Sun, Jipeng
    Wang, Yanbin
    Du, Chuanhang
    Peng, Feifei
    Gao, Shiqing
    Chen, Qiang
    Powiązania:
    https://bibliotekanauki.pl/articles/27765052.pdf
    Data publikacji:
    2023
    Wydawca:
    Polska Akademia Nauk. Czasopisma i Monografie PAN
    Tematy:
    ZL114A alloy
    plaster-mold casting
    tensile properties
    microstructure
    fracture
    Opis:
    The tensile properties and microstructures of ZL114A alloy component with a complex shape are investigated at room temperature and 200°C, using the tensile tests, scanning electron microscopy and electron backscattering diffraction. Both thin wall and thick structure exhibit excellent properties, of which max ultimate tensile strength and elongation at break reach 314 MPa and 2.5% at room temperature, respectively. The ultimate tensile strengths of thin wall are 40 MPa and 25 MPa greater than those of thick structure at room temperature and 200°C, respectively. Moreover, the eutectic Si phases of thin wall exhibit a predominantly spherical morphology while of the morphology of thick structure are rod-like, resulting in the different mechanical properties between thin wall and thick structure. The fracture morphologies of thin wall and thick structure are studied to explain the difference in performance between thin wall and thick structure.
    Źródło:
    Archives of Metallurgy and Materials; 2023, 68, 2; 585--589
    1733-3490
    Pojawia się w:
    Archives of Metallurgy and Materials
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Time-frequency Representation -enhanced Transfer Learning for Tool Condition Monitoring during milling of Inconel 718
    Autorzy:
    Zhou, Yuqing
    Sun, Wei
    Ye, Canyang
    Peng, Bihui
    Fang, Xu
    Lin, Canyu
    Wang, Gonghai
    Kumar, Anil
    Sun, Weifang
    Powiązania:
    https://bibliotekanauki.pl/articles/24200823.pdf
    Data publikacji:
    2023
    Wydawca:
    Polska Akademia Nauk. Polskie Naukowo-Techniczne Towarzystwo Eksploatacyjne PAN
    Tematy:
    tool condition monitoring
    time-frequency analysis
    Markov Transition Field
    transfer learning
    Opis:
    Accurate tool condition monitoring (TCM) is important for the development and upgrading of the manufacturing industry. Recently, machine-learning (ML) models have been widely used in the field of TCM with many favorable results. Nevertheless, in the actual industrial scenario, only a few samples are available for model training due to the cost of experiments, which significantly affects the performance of ML models. A time-series dimension expansion and transfer learning (TL) method is developed to boost the performance of TCM for small samples. First, a time-frequency Markov transition field (TFMTF) is proposed to encode the cutting force signal in the cutting process to two-dimensional images. Then, a modified TL network is established to learn and classify tool conditions under small samples. The performance of the proposed TFMTF-TL method is demonstrated by the benchmark PHM 2010 TCM dataset. The results show the proposed method effectively obtains superior classification accuracies for small samples and outperforms other four benchmark methods.
    Źródło:
    Eksploatacja i Niezawodność; 2023, 25, 2; art. no. 165926
    1507-2711
    Pojawia się w:
    Eksploatacja i Niezawodność
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-13 z 13

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