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Wyświetlanie 1-15 z 27
Tytuł:
Identification of a linear epitope in the capsid protein of goose astrovirus with monoclonal antibody
Autorzy:
Dai, G.
Huang, X.
Liu, Q.
Li, Y.
Zhang, L.
Han, K.
Yang, J.
Liu, Y.
Xue, F.
Zhao, D.
Powiązania:
https://bibliotekanauki.pl/articles/16647377.pdf
Data publikacji:
2022
Wydawca:
Polska Akademia Nauk. Czasopisma i Monografie PAN
Tematy:
epitope
goose astrovirus
capsid protein
monoclonal antibody
Opis:
Goose astrovirus (GoAstV) is a novel avastrovirus that typically causes gosling gout and results in 2 to 20% mortality. GoAstV capsid protein is the sole structural protein, which is responsible for viral attachment, assembly, maturation as well as eliciting host antibodies. However, the epitopes within capsid protein have not been well studied. In this study, a monoclonal antibody, named 1D7, was generated against GoAstV capsid protein by hybridoma technology. Western blot results showed that this MAb could react with recombinant capsid protein expressed in E. coli. Also, it recognized the precursor of capsid protein, VP90 and VP70, in GoAstV-infected cells. Besides, excellent specificity of MAb 1D7 was further demonstrated in indirect immunofluorescence assay and immunohistochemical analysis. Epitope mapping results revealed that MAb 1D7 recognized the epitope 33QKVY 36 within Cap protein. Sequence alignment indicated that 33QKVY 36 is a conserved epitope among the isolates of goose astrovirus type 2 (GoAstV-2), suggesting the potential for its use in GoAstV-2 specific diagnostic assay. These findings may provide some insight into a function of the GoAstV capsid protein and further contribute to the development of diagnostic methods for GoAstV infection.
Źródło:
Polish Journal of Veterinary Sciences; 2022, 25, 4; 579-587
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Electron beam technology for biogas and biofertilizer generation at municipal resource recovery facilities
Autorzy:
Chmielewski, Andrzej G.
Sudlitz, Marcin
Han, Bumsoo
Pillai, Suresh D.
Powiązania:
https://bibliotekanauki.pl/articles/2055685.pdf
Data publikacji:
2021
Wydawca:
Instytut Chemii i Techniki Jądrowej
Tematy:
biogas
biofertilizer
cogeneration
electron beam
ionizing radiation
municipal wastewater plant sludge
phosphorus
resource recovery facility
sludge hygenization
Opis:
In the era of circular economies, municipal wastewater treatment plants (WWTPs) are viewed as resource recovery facilities. At the very minimum, the targeted resources are water, biogas, and phosphorus. However, municipal wastewater streams (sludge and effluent) need to be adequately treated to eliminate the potential for the transmission of microbial pathogens including protozoa, bacteria, and viruses. This paper presents the results from a study demonstrating the use of electron beam technology for sludge hygenization and enhanced methane (biogas) production using municipal wastewater samples. Cogeneration of heat for fertilizer drying and granulation and electricity for powering the electron beam system are also demonstrated.
Źródło:
Nukleonika; 2021, 66, 4; 213--219
0029-5922
1508-5791
Pojawia się w:
Nukleonika
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The effect of hip dysplasia on some biochemical parameters, oxidative stress factors and hematocrit levels in dogs
Autorzy:
Polat, E.
Han, M.C.
Kaya, E.
Yilmaz, S.
Kayapinar, S.D.
Coskun, S.
Yildirim, A.
Can, U.K.
Powiązania:
https://bibliotekanauki.pl/articles/2087150.pdf
Data publikacji:
2021
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
alkaline phosphatase
hematocrit
hip dysplasia
creatine kinase
oxidative stress
Źródło:
Polish Journal of Veterinary Sciences; 2021, 24, 4; 473-478
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
An improved resonant thermal converter based on micro-bridge resonator
Autorzy:
Dong, L.
Han, J.
Zhang, P.
Zhao, Z.
Cheng, B.
Han, D.
Powiązania:
https://bibliotekanauki.pl/articles/220878.pdf
Data publikacji:
2018
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
thermoelectric transfer difference
micro-bridge resonator
thermal converter
bifilar heater
Opis:
This paper presents the design, fabrication and testing of an improved thin-film thermal converter based on an electro-thermally excited and piezo-resistively detected micro-bridge resonator. The resonant thermal converter comprises a bifilar heater and an opposing micro-bridge resonator. When the micro-bridge resonator absorbs the radiant heat from the heater, its axial strain changes, then its resonant frequency follows. Therefore the alternating voltage or current can be transferred to the equivalent DC quantity. A non-contact temperature sensing mechanism eliminates heat loss from thermopiles and reduces coupling capacitance between the temperature sensor and the heater compared with traditional thin-film thermal converters based on thermopiles. In addition, the quasi-digital output of the resonant thin-film thermal converter eliminates such problems as intensity fluctuations associated with analogue signals output by traditional thin-film thermal converters. Using the fast-reversed DC (FRDC) method, the thermoelectric transfer difference, which determines the frequency-independent part of the ac-dc transfer difference, is evaluated to be as low as 1.1 · 10-6. It indicates that the non-contact temperature sensing mechanism is a feasible method to develop a high-performance thermal converter.
Źródło:
Metrology and Measurement Systems; 2018, 25, 4; 715-725
0860-8229
Pojawia się w:
Metrology and Measurement Systems
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Development of Hydrophobic Surgical Forceps using Powder Injection Molding and Surface Treatment
Autorzy:
Park, D. Y.
Park, B. R.
Gal, C. W.
Lin, D.
Han, J. S.
Jeong, M.-S.
Bollina, R.
Hwang, W.
Park, S. J.
Powiązania:
https://bibliotekanauki.pl/articles/352093.pdf
Data publikacji:
2018
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
powder injection molding
hydrophobic
debinding
sintering
surface treatment
Opis:
Hydrophobic surgical forceps of end-effectors for laparoscopic operations or minimally invasive surgery were developed through powder injection molding (PIM) and surface treatment. Processing conditions for mixing, debinding, and sintering were investigated to produce defect-free components. An optimum solid loading was determined by torque rheometry experiments. The optimized processing conditions for debinding and sintering were designed through the measurement of weight loss and shrinkage behavior by thermogravimetric analysis and dilatometry experiments. After producing the surgical forceps based on the optimized processing conditions via PIM, surface treatment was carried out to generate the hydrophobic structure on the surface.
Źródło:
Archives of Metallurgy and Materials; 2018, 63, 1; 473-480
1733-3490
Pojawia się w:
Archives of Metallurgy and Materials
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Effect of experimental infections of various Tembusu virus strains isolated from geese, ducks and chickens on ducklings
Autorzy:
Xu, T.
Huang, X.
Li, Y.
Liu, Q.
Liu, X.
Han, K.
Liu, Y.
Yang, J.
Zhao, D.
Bi, K.
Sun, W.
Powiązania:
https://bibliotekanauki.pl/articles/2087719.pdf
Data publikacji:
2018
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
goose-derived
duck-derived
chicken-derived
Tembusu virus
pathogenicity
Źródło:
Polish Journal of Veterinary Sciences; 2018, 21, 2; 389-396
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Prediction of the height of overburden fractured zone in deep coal mining: case study
Prognozowanie wysokości strefy spękań w warstwach nadkładu w podziemnych kopalniach węgla: studium przypadku
Autorzy:
Han, Y.
Cheng, J.
Huang, Q.
Zou, D. H. S.
Zhou, J.
Huang, S.
Long, Y.
Powiązania:
https://bibliotekanauki.pl/articles/218745.pdf
Data publikacji:
2018
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
wysokość strefy spękań w warstwie nadkładu
symulacje numeryczne
układ ortogonalny
pomiary in-situ
the hei ght of overburden fractured zone
numerical simulation
orthogonal design
in-situ test
Opis:
In the process of coal extraction, a fractured zone is developed in the overburden above the goaf. If the fractured zone is connected with an aquifer, then water inrush may occur. Hence, research and analysis of the height of overburden fractured zone (HOFZ) are of considerable significance. This study focuses on the HOFZ determination in deep coal mining. First, general deformation failure characteristics of overburden were discussed. Second, a new method, numerical simulation by orthogonal design(NSOD), have been proposed to determinate the HOFZ in deep coal mining. Third, the validity of NSOD is verified in the practical application, compared with empiric al formula in Chinese Regulations and in-situ test. These three methods were applied to determine the HOFZ of working face No. 111303 in No. 5 coal mine. The pre dicted HOFZ of NSOD is found to be similar to the result of the in-situ test (8.9% relative error), whereas the HOFZ calculated by the empirical formula has extremely large error (25.7% relative error). Results show that the NSOD can reliably predict the HOFZ in deep coal mining and reduce time and expenses required for in-situ test.
W trakcie prowadzenia wydobycia węgla w warstwie skalnej leżącej ponad zrobami powstaje strefa spękań. Jeśli nieciągłości te związane są z formacjami wodonośnymi, może nastąpić nagły wypływ wód do kopalni. Stąd też waga problemu i konieczność badania wysokości strefy spękań w warstwach nadkładu zalegających nad wyrobiskiem. W pracy tej główny nacisk położono na określenie wysokości strefy spękań warstwy nadkładu zalegającej ponad wyrobiskiem w kopalni podziemnej. W pierwszej części pracy przedstawiono główne charakterystyki powstawania deformacji i pęknięć w warstwach nadkładu. Następnie zaproponowano nową metodę symulacji numerycznych w układzie ortogonalnym i jej wykorzystanie do określania wysokości strefy spękań w warstwach nadkładu w kopalni podziemnej. Następnie powyższą metodę zweryfikowano w praktycznym zastosowaniu, jako poziomy odniesienia wykorzystano odpowiednie wzory empiryczne określone w przepisach obowiązujących w Chinach oraz wyniki pomiarów in-situ. Powyższe trzy metody zastosowane zostały do określenia wysokości strefy spękań w warstwach nadkładu przy prowadzeniu ściany 111303 w kopalni węgla nr 5. Wyniki obliczeń wysokości strefy spękań uzyskane w oparciu o zaproponowaną nową metodę w dużym stopniu pokrywały się z wynikami pomiarów in-situ (błąd względny 8.9%); podczas gdy obliczenia wysokości strefy spękań w oparciu o odpowiednie wzory empiryczne obarczone były dużym błędem (błąd względny 25.7%). Uzyskane wyniki wskazują wiarygodność obliczeń w oparciu o zaproponowaną metodę, co pozwoli na ograniczenie czasu i kosztów związanych z wykonywaniem pomiarów in-situ.
Źródło:
Archives of Mining Sciences; 2018, 63, 3; 617-631
0860-7001
Pojawia się w:
Archives of Mining Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Transcriptomic landscape of Dendrobium huoshanense and its genes related to polysaccharide biosynthesis
Autorzy:
Han, R.
Xie, D.
Tong, X.
Zhang, W.
Liu, G.
Peng, D.
Yu, N.
Powiązania:
https://bibliotekanauki.pl/articles/57911.pdf
Data publikacji:
2018
Wydawca:
Polskie Towarzystwo Botaniczne
Opis:
Dendrobium huoshanense has long been used to treat various diseases in oriental medicine. In order to study its gene expression profile, transcripts involved in the biosynthesis of precursors of polysaccharides, as well as mechanisms underlining morphological differences between wild and cultivated plants, three organs of both wild type and cultivated D. huoshanense were collected and sequenced by Illumina HiSeq4000 platform, yielding 919,409,540 raw reads in FASTQ format. After Trinity de novo assembly and quality control, 241,242 nonredundant contigs with the average length of 967.5 bp were generated. qRT-PCR experiment on the selected transcripts showed the transcriptomic data were reliable. BLASTx was conducted against NR, SwissProt, String, Pfam, and KEGG. Gene ontology annotation revealed more than 40,000 contigs assigned to catalytic activity and metabolic process, suggesting its dynamic physiological activities. By searching KEGG pathway, six genes potentially involved in mannose biosynthetic pathway were retrieved. Gene expression analysis for stems between wild and cultivated D. huoshanense resulted in 956 genes differentially expressed. Simple sequence repeats (SSRs) analysis revealed 143 SSRs with the unit size of 4 and 3,437 SSRs the size of 3. The obtained SSRs are the potential molecular markers for discriminating distinct cultivars of D. huoshanense.
Źródło:
Acta Societatis Botanicorum Poloniae; 2018, 87, 1
0001-6977
2083-9480
Pojawia się w:
Acta Societatis Botanicorum Poloniae
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
An active fault-tolerant control framework against actuator stuck failures under input saturations
Autorzy:
Qi, X.
Theilliol, D.
He, Y.
Han, J.
Powiązania:
https://bibliotekanauki.pl/articles/331084.pdf
Data publikacji:
2017
Wydawca:
Uniwersytet Zielonogórski. Oficyna Wydawnicza
Tematy:
fault tolerant control
actuator stuck failure
actuator constraints
reference redesign
linear matrix inequality
sterowanie tolerujące uszkodzenia
projekt odniesienia
liniowa nierówność macierzowa
Opis:
In this paper, a control framework including active fault-tolerant control (FTC) and reference redesign is developed subject to actuator stuck failures under input saturations. FTC synthesis and reference redesign approaches are proposed to guarantee post-fault system safety and reference reachability. Then, these features are analyzed under both actuator stuck failures and constraints before fault-tolerant controller switches. As the main contribution, actuator stuck failures and constraints are unified so that they can be easily considered simultaneously. By means of transforming stuck failures into actuator constraints, the post-fault system can be regarded as an equivalent system with only asymmetrical actuator constraints. Thus, methods against actuator saturations can be used to guarantee regional stability and produce the stability region. Based on this region, stuck compensation is analyzed. Specifically, an unstable open-loop system is considered, which is more challenging. Furthermore, the method is extended to a set-point tracking problem where the reachability of the original reference can be evaluated. Then, a new optimal reference will be computed for the post-fault system if the original one is unreachable. Finally, simulation examples are shown to illustrate the theoretical results.
Źródło:
International Journal of Applied Mathematics and Computer Science; 2017, 27, 4; 749-761
1641-876X
2083-8492
Pojawia się w:
International Journal of Applied Mathematics and Computer Science
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Understanding the Oxide Dispersion Behavior of Yttria in Metal Matrix of MA956 Alloy through High-Energy Milling and Hot Press Sintering
Autorzy:
Swain, B.
Han, D.
Kim, G.-H.
Kong, M.-S.
Ahn, B.
Powiązania:
https://bibliotekanauki.pl/articles/351770.pdf
Data publikacji:
2017
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
ODS
mechanical alloying
high-energy milling
oxide particle distribution
Opis:
MA956 (Fe-Cr-Al) alloy powder was high-energy ball milled with various amount of yttria contents (1,2,3, and 4 wt.%) to fabricate an oxide dispersion strengthened alloy. The milled powders were then consolidated using hot press sintering at 1150°C. The surface morphology and crystal structure of MA956 powder during the high-energy milling depending on the yttria contents was investigated using particle size analysis, X-ray diffraction, and scanning electron microscopy. The microstructural analysis of sintered alloy was performed using transmission electron microscopy and energy dispersive spectroscopy to evaluate the dispersion behavior of yttrium oxide. The results showed that, as yttria contents increased, the oxide particles became finer and are uniformly distributed during the high-energy milling. However, after the sintering, the oxide particles were coarsened with more than 3 wt.% of yttria addition.
Źródło:
Archives of Metallurgy and Materials; 2017, 62, 2B; 1377-1381
1733-3490
Pojawia się w:
Archives of Metallurgy and Materials
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
A Study on Discharge Characteristics by Using MF and RF Power in Remote Dielectric Barrier Discharge
Autorzy:
Kim, D.
Shim, Y.
Kim, H.
Han, J.
Powiązania:
https://bibliotekanauki.pl/articles/1398758.pdf
Data publikacji:
2016-04
Wydawca:
Polska Akademia Nauk. Instytut Fizyki PAN
Tematy:
52.77.Bn
Opis:
We have developed an atmospheric pressure plasma apparatus of remote dielectric barrier discharge (RDBD) applicable for a large area. We have systematically studied the characteristics of medium frequency (MF, 40 kHz) and radio frequency (RF, 13.56 MHz) discharge using an optical emission spectroscope. Nitrogen (N₂) and argon (Ar) gases were used in the MF and RF discharge excitation, respectively, in a mixture with clean dry air (CDA). The peak of oxygen radical (O*₂) appears at 259.3 nm when the RDBD is employed. Furthermore, intensive peaks are observed at gas ratios of N₂:CDA=100:1 in MF excitation and at gas ratios of Ar:CDA=70:0.5 in RF discharge excitation. On the other hand, the contact angle shows about 5° in PET samples after the RDBD treatment using the RF and MF discharge excitation. Surface analyses of polyethylene terephthalate (PET) samples were carried out using an atomic force microscope and X-ray photoelectron spectroscope.
Źródło:
Acta Physica Polonica A; 2016, 129, 4; 707-710
0587-4246
1898-794X
Pojawia się w:
Acta Physica Polonica A
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Generation and characterization of a monoclonal antibody against duck Tembusu virus envelope protein
Autorzy:
Han, K.
Zhao, D.
Liu, Y.
Liu, Q.
Huang, X.
Yang, J.
Bi, K.
Xu, T.
Li, Y.
Powiązania:
https://bibliotekanauki.pl/articles/31495.pdf
Data publikacji:
2016
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
Duck Tembusu virus (DTMUV) is a newly emerging pathogenic flavivirus that has caused massive economic losses to the duck industry in China. Envelope (E) protein of DTMUV is an important structural protein, which is able to induce protective immune response in target animals and can be used as specific serological diagnosis tool. In this study, a novel monoclonal antibody, designated mAb 3E9, was generated against DTMUV E protein. It is positive in indirect ELISA against both His-E protein and the purified whole viral antigen. Also, this mAb showed positive reaction with DTMUV in Western blot and indirect immunofluorescence assay, and the isotype was IgG1. End-point neutralizing assay performed in BHK-21 cells revealed that the neutralization titer of 3E9 against DTMUV JS804 strain reached 1:50. Furthermore, functional studies revealed that 3E9 blocks infection of DTMUV at a step on viral attachment. The anti-E mAbs produced in the present work may be valuable in developing an antigen-capture ELISA test for antigen detection or a competitive ELISA test for antibody detection or therapeutic medicine for DTMUV in poultry.
Źródło:
Polish Journal of Veterinary Sciences; 2016, 19, 4
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Double Step Sintering Behavior Of 316L Nanoparticle Dispersed Micro-Sphere Powder
Dwuetapowe spiekanie mikrosferycznych nanocząstek proszku 316L
Autorzy:
Jeon, B.
Sohn, S. H.
Lee, W.
Han, C.
Kim, Y. D.
Choi, H.
Powiązania:
https://bibliotekanauki.pl/articles/355221.pdf
Data publikacji:
2015
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
316L stainless steel
nanoparticle dispersed micro-sphere
pulse wire explosion
sintering
stal nierdzewna 316L
spiekanie dwuetapowe
mikrosferyczne nanocząstki
Opis:
316L stainless steel is a well-established engineering material and lots of components are fabricated by either ingot metallurgy or powder metallurgy. From the viewpoints of material properties and process versatility, powder metallurgy has been widely applied in industries. Generally, stainless steel powders are prepared by atomization processes and powder characteristics, compaction ability, and sinterability are quite different according to the powder preparation process. In the present study, a nanoparticle dispersed micro-sphere powder is synthesized by pulse wire explosion of 316L stainless steel wire in order to facilitate compaction ability and sintering ability. Nanoparticles which are deposited on the surface of micro-powder are advantageous for a rigid die compaction while spherical micro-powder is not to be compacted. Additionally, double step sintering behavior is observed for the powder in the dilatometry of cylindrical compact body. Earlier shrinkage peak comes from the sintering of nanoparticle and later one results from the micro-powder sintering. Microstructure as well as phase composition of the sintered body is investigated.
Źródło:
Archives of Metallurgy and Materials; 2015, 60, 2B; 1155-1158
1733-3490
Pojawia się w:
Archives of Metallurgy and Materials
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Multi-grid model for crowd’s evacuation in ships based on cellular automata
Autorzy:
Chen, M.
Han, D.
Powiązania:
https://bibliotekanauki.pl/articles/963361.pdf
Data publikacji:
2015
Wydawca:
Politechnika Gdańska. Wydział Inżynierii Mechanicznej i Okrętownictwa
Tematy:
crowds’ evacuation in ships
multi-grid model
cellular automata model
finer lattice
interaction force
Opis:
In order to enhance the authenticity and accuracy simulation of passengers’ evacuation in ships, a new multi-grid model is proposed on the basis of cellular automata theory. By finer lattice the multi-grid model could enhance the continuity of passengers’ track and the precision of boundary’s qualification compared with traditional cellular automata model. Attraction, repulsion and friction are also quantized in the multi-grid model to present the impact of interaction force among pedestrians. Furthermore, crowd’s evacuation simulated by traditional cellular automata and multi-grid model in single exit room and typical cabin environment have been taken as examples to analyze crowd’s motion laws. It is found that the laws of passengers’ evacuation simulated by the two models are similar, and the simulation authenticity and accuracy is enhanced by the multi-grid model.
Źródło:
Polish Maritime Research; 2015, S 1; 75-81
1233-2585
Pojawia się w:
Polish Maritime Research
Dostawca treści:
Biblioteka Nauki
Artykuł
Wyświetlanie 1-15 z 27

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