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    Wyświetlanie 1-12 z 12
    Tytuł:
    Isolation, characterisation and biological activity of melanin from Exidia nigricans
    Autorzy:
    Łopusiewicz, Łukasz
    Powiązania:
    https://bibliotekanauki.pl/articles/1178254.pdf
    Data publikacji:
    2018
    Wydawca:
    Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
    Tematy:
    Exidia nigricans
    antimicrobial
    antioxidant
    light barrier
    melanin
    pigment
    Opis:
    The aim of present study was isolation and characteriation of raw and purified melanin from Exidia nigricans. Native melanin was isolated from the fresh E. nigricans fruiting bodies by alkaline extraction. Obtained pigment was purifed by acid hydrolysis and washed by organic solvents. Chemical tests, FT-IR and Raman spectroscopy analysis were conducted to determine the melanin nature of the isolated pigment. UV-Vis, transmittance and colour properties were evaluated. Antioxidant activity was determined using ABTS and antibacterial activity by a well diffusion method. The results of the study demonstrated that melanins isolated from E. nigricans had antioxidant, light barrier and antibacterial properties. A purified form of melanin offered better light properties and higher antioxidant activity than the raw form. Both melanins inhibited the growth of E. facealis and P. aeruginosa. This study revealed that E. nigricans may be considered as a promising source of natural melanin. Isolated pigments presented all the physical and chemical properties common to natural and synthetic melanins. Raw and purified melanins showed differences in chemical composition, antioxidant activity and light barrier properties. Melanin may play pivotal role in physiology of E. nigricans protecting it against UV radiation and dessication.
    Źródło:
    World Scientific News; 2018, 91; 111-129
    2392-2192
    Pojawia się w:
    World Scientific News
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Scleroderma citrinum melanin: isolation, purification, spectroscopic studies with characterization of antioxidant, antibacterial and light barrier properties
    Autorzy:
    Łopusiewicz, Łukasz
    Powiązania:
    https://bibliotekanauki.pl/articles/1156260.pdf
    Data publikacji:
    2018
    Wydawca:
    Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
    Tematy:
    Scleroderma citrinum
    antibacterial
    antioxidant
    earthball
    light barrier
    melanin
    Opis:
    The aim of this study was to isolate and evaluate biological properties of raw and purified melanins isolated from Scleroderma citrinum. Native melanin was isolated from the gleba of fresh S. citrinum fruiting bodies by alkaline extraction. Obtained pigment was purifed by acid hydrolysis and washed by organic solvents. Chemical tests, FT-IR and Raman spectroscopy analysis were conducted to determine the melanin nature of the isolated pigments. UV-Vis, transmittance, total polyphenolic content and colour properties were evaluated. Antioxidant activity was determined using ABTS and antibacterial activity by a well diffusion method. The results of the study demonstrated that melanins isolated from S. citrinum had antioxidant, light barrier and antibacterial properties. A purified form of melanin offered better light properties and higher antioxidant activity than the raw form. Both melanins inhibited the growth of E. facealis and P. aeruginosa. This study revealed that S. citrinum may be considered as a promising source of natural melanin. Isolated pigments presented all the physical and chemical properties common to natural and synthetic melanins. Raw and purified melanins showed differences in chemical composition, antioxidant activity and light barrier properties. Results suggest that, melanins from S. citrinum possess remarkable therapeutic action and could be applied in the food, cosmetics and pharmaceutical industries.
    Źródło:
    World Scientific News; 2018, 94, 2; 115-130
    2392-2192
    Pojawia się w:
    World Scientific News
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    The isolation, purification and analysis of the melanin pigment extracted from Armillaria mellea rhizomorphs
    Autorzy:
    Łopusiewicz, Łukasz
    Powiązania:
    https://bibliotekanauki.pl/articles/1177769.pdf
    Data publikacji:
    2018
    Wydawca:
    Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
    Tematy:
    Armillaria mellea
    antibacterial
    antioxidant
    light barrier
    melanin
    rhizomorphs
    Opis:
    The aim of present study was isolation and characteriation of raw and purified melanin from Armillaria mellea rhizomorphs. Native melanin was isolated from the rhizomorphs of A. mellea by alkaline extraction. Obtained pigment was purifed by acid hydrolysis and washed by organic solvents. Chemical tests, FT-IR and Raman spectroscopy analysis were conducted to determine the melanin nature of the isolated pigment. UV-Vis, transmittance and colour properties were evaluated. Antioxidant activity was determined using ABTS and antibacterial activity by a well diffusion method. The results of the study demonstrated that melanins isolated from A. mellea rhizomorphs had antioxidant, light barrier and antibacterial properties. A purified form of melanin offered better light properties and higher antioxidant activity than the raw form. Both melanins showed antimicrobial activity, raw melanin form had broader activity compared to the pure form. This study revealed that A. mellea rhizomorphs may be considered as a promising source of natural melanin. Isolated pigments presented all the physical and chemical properties common to natural and synthetic melanins. Raw and purified melanins showed differences in chemical composition, antioxidant activity and light barrier properties. Results of this study suggest that, melanins from A. mellea could be applied in the food, cosmetics and pharmaceutical industries.
    Źródło:
    World Scientific News; 2018, 100; 135-153
    2392-2192
    Pojawia się w:
    World Scientific News
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Antifungal activity of PLA foils covered with ethylocelulose containing essential oils
    Autorzy:
    Łopusiewicz, Łukasz
    Mizielińska, Małgorzata
    Powiązania:
    https://bibliotekanauki.pl/articles/1113918.pdf
    Data publikacji:
    2017
    Wydawca:
    Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
    Tematy:
    active coatings
    antifungal properties
    essential oils
    Opis:
    The purpose of the research work was to examine the antifungal properties of cumin, rosemary and fennel essential oils. The goal of the study was also to determine antifungal activity of coatings containing the essential oils. The results of the study demonstrated that cumin, rosemary and fennel essential oils exhibited antifungal properties. The most sensitive strains were Botrytis cinerea and Mucor circinelloides. The cumin oil was found to be the most active. The growth of Botrytis cinerea and Mucor circinelloides was not observed even on medium containing 0.1% of cumin oil. The growth of Aspergillus niger, Aspergillus clavatus and Rhizopus oryzae was not noticed on mediums containig 0.5% of the oil. The similar results were obtained for fennel essential oil. 0.5% concentration of the oil in medium inhibited the growth of Botrytis cinerea, Mucor circinelloides, Aspergillus clavatus, Rhizopus oryzae. The rosemary oil did not inhibited the growth of fungi strains in concentration of 0,1%.
    Źródło:
    World News of Natural Sciences; 2017, 12; 27-32
    2543-5426
    Pojawia się w:
    World News of Natural Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    "Czarne złoto" - melaniny w życiu człowieka
    "Black gold" - melanins in human life
    Autorzy:
    Łopusiewicz, Łukasz
    Lisiecki, Sławomir
    Powiązania:
    https://bibliotekanauki.pl/articles/1034438.pdf
    Data publikacji:
    2016
    Wydawca:
    Polskie Towarzystwo Przyrodników im. Kopernika
    Tematy:
    melaniny
    melanogeneza
    melanosomy
    UV
    Opis:
    Melaniny to ogólna nazwa grupy wielkocząsteczkowych barwników odpowiedzialnych za ciemną pigmentację organizmów. Powstają w wyniku oksydacyjnej polimeryzacji związków fenolowych i indolowych. Są to jedne z najbardziej powszechnych, heterogenicznych i odpornych na działanie różnych czynników pigmentów obecnych w przyrodzie. U człowieka powstają w melanosomach będących specyficznymi organellami melanocytów, komórek wyspecjalizowanych w przeprowadzaniu złożonego procesu melanogenezy. Ich rola w życiu człowieka jest wieloaspektowa. Chronią przed szkodliwym skutkiem nadmiernej ekspozycji na promieniowanie UV i powstającymi pod jego wpływem reaktywnymi formami tlenu. Są odpowiedzialne za pigmentację skóry, włosów, tęczówki. Biorą udział w prawidłowym przebiegu procesów widzenia i słyszenia oraz ochronie mózgu przed procesami neurodegeneracyjnymi. Są jednym z czynników wirulencji mikroorganizmów patogennych dla człowieka. Są obecne w niektórych produktach spożywczych, pozytywnie wpływając na ich działanie prozdrowotne. Znalazły również zastosowanie w nowoczesnej medycynie i technologii.
    Melanin is a general name of macromolecular dyes responsible for the dark pigmentation of organisms. They are products of oxidative polymerization of phenolic and indolic compounds, the most common, heterogenous and resistant to various factors pigments found in the nature. In man they are synthetized in melanosomes that are specific organelles of melanocytes, cells specialized in carrying out the complex process of melanogenesis. The role of melanin in human life is multiplex. Melanins protect against the harmful effects of excessive exposure to UV radiation and reactive oxygen species. They are responsible for skin, hair and iris pigmentation. They take part in the normal processes of vision and hearing and protect the brain against neurodegenerative processes. Melanins are one of the virulence factors of some pathogenic microorganisms. They are present in certain food products, thus improving their health-promoting effect. Melanins are also used in modern medicine and technology.
    Źródło:
    Kosmos; 2016, 65, 4; 621-629
    0023-4249
    Pojawia się w:
    Kosmos
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    The application of melanin modified gelatin coatings for packaging and the oxidative stability of pork lard
    Autorzy:
    Łopusiewicz, Łukasz
    Jędra, Filip
    Bartkowiak, Artur
    Powiązania:
    https://bibliotekanauki.pl/articles/1177712.pdf
    Data publikacji:
    2018
    Wydawca:
    Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
    Tematy:
    antioxidant
    gelatin
    lard
    lipid oxidation
    melanin
    packaging
    Opis:
    The influence of gelatin coatings modified with fungal melanin on pork lard oxidative stability was studied. The lard was coated with gelatin coatings containing 0.1%; 0.5%; 1% of fungal melanin and control gelatin coating devoid of melanin. The peroxide values (POV), iodine values (IV) and acid values (AV) were studied after 7, 14 and 21 days storage in controlled conditions. Lard covered with modified coatings had lowered oxidative rancidity. Hence, modified coatings containing fungal melanin can be use effectively for the prevention of lard oxidation.
    Źródło:
    World Scientific News; 2018, 101; 108-119
    2392-2192
    Pojawia się w:
    World Scientific News
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    The influence of PU foams modification on the efficiency of Citrobacter freundii cells immobilization
    Autorzy:
    Mizielińska, Małgorzata
    Kowalska, Urszula
    Łopusiewicz, Łukasz
    Powiązania:
    https://bibliotekanauki.pl/articles/1178364.pdf
    Data publikacji:
    2017
    Wydawca:
    Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
    Tematy:
    Citrobacter freundii
    bacterial adhesion
    bioimmobilization
    polyurethane foam
    Opis:
    The purpose of the study was to modify PU (polyurethane) foams to increase the efficiency of Citrobacter freundii cells adhesion. The immobilization can contribute to better productivity during the bioconversion of glycerol to 1,3-propanediol (1,3-PD). The results of the study showed that C. freundii immobilized on foams with organic additives could decompose more glycerol and produce more 1,3-PD than cells immobilized on pure foams. The immobilization made production in long-term operations or repeated runs possible. The results of the experiments have also proved that the foams with additives, especially with wood chips and peanut shells, would be the most suitable carriers because they allow the immobilization of a higher number of bacterial cells. It was demonstrated that the initial number of C. freundii cells in all samples (before incubation) was 1.2×106 CFU/mL. The results showed that the accumulation of bacterial cells in the control sample with pure PUF (polyurethane foams) was 1.5×107 CFU/mL after 24 hours of incubation (the number of bacterial cells attached to surface of foams). It was determined that the accumulation of bacterial cells in samples with 15% of additives at 30 °C increased to 2.17×107/ 3.37×107/ 1.25×108/ 1.65×108/ 5.65×107 CFU (PUFbtb, PUFab, PUFwch, PUFps, PUFrc) CFU/mL after 24 hours of incubation.
    Źródło:
    World Scientific News; 2017, 77, 2; 211-225
    2392-2192
    Pojawia się w:
    World Scientific News
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Polihydroksyalkanolany - obiecujące polimery biodegradowalne
    Polyhydroxyalkanoates - promising biodegradable polymers
    Autorzy:
    Mizielińska, Małgorzata
    Łopusiewicz, Łukasz
    Soból, Marcin
    Powiązania:
    https://bibliotekanauki.pl/articles/1033731.pdf
    Data publikacji:
    2018
    Wydawca:
    Polskie Towarzystwo Przyrodników im. Kopernika
    Tematy:
    biopolymers
    microorganisms
    plastics
    polyhydroxyalkanoates
    biopolimery
    mikroorganizmy
    polihydroksyalkanolany
    tworzywa sztuczne
    Opis:
    W pracy omówiono polihydroksyalkanolany, biopolimery o ogromnym potencjale. Scharakteryzowano ich właściwości i zastosowanie, zwracając uwagę na takie ich zalety jak biozgodność (brak toksyczności w stosunku do organizmu), podatność na biodegradację, czy właściwości termoplastyczne, zbliżone do tworzyw sztucznych. Niestety, pomimo wielu zalet, PHA nie są powszechnie wykorzystywane, głównie ze względu na wysoki koszt ich wytwarzania i oczyszczania. Dlatego w celu obniżenia kosztów podejmuje się działania w kierunku zastąpienia podczas hodowli drobnoustrojów źródła węgla tanimi materiałami odpadowymi. Kolejnym sposobem zmniejszenia kosztów produkcji PHA jest wykorzystanie modyfikowanych genetycznie mikroorganizmów. Alternatywą może być także poszukiwanie nowych metod wyodrębniania biopolimeru z komórek. Dobrym pomysłem może być także łączenie chemicznych i mechanicznych metod oczyszczania PHA. PHA bardzo często wykorzystywane są w medycynie, zastępując tworzywa sztuczne, gdzie istotne są właściwości materiałów oraz ich podatność na biodegradację. Niestety, ze względu na cenę, do wytwarzania polimerowych przedmiotów użytku codziennego, polimery syntetyczne wykorzystywane są częściej.
    This paper discusses properties of polyhydroxyalkanoates (PHA), biopolymers with a huge potential for practical applications. PHA have a number of advantages such as biocompatibility (no toxicity for organism), biodegradability and thermoplastic properties similar to those of synthetic plastics. Unfortunately, despite these advantages PHA are not widely used owing to the high costs of their preparation and purification. Therefore, in order to reduce these costs efforts are being undertaken to replace traditional carbon sources for cultivation of microorganisms by cheaper waste materials. Another way to reduce the cost of PHA production is the use of genetically modified microorganisms. An alternative approach may be also a search for new methods for extraction of these biopolymer from the cells including combination of chemical and mechanical methods of purification. PHA are very often used in medicine, because of their properties and biodegradability. In this area they substitute plastics. Unfortunately, costs of production from PHA polymeric objects for everyday use are still too high, so synthetic polymers remain predominantly in use.
    Źródło:
    Kosmos; 2018, 67, 2; 299-306
    0023-4249
    Pojawia się w:
    Kosmos
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Evaluation of petroleum hydrocarbons adsorbtion and biodegradation by Pseudomonas aeruginosa cells entrapped into silica-alginate beads
    Autorzy:
    Mizielińska, Małgorzata
    Kowalska, Urszula
    Łopusiewicz, Łukasz
    Powiązania:
    https://bibliotekanauki.pl/articles/1179265.pdf
    Data publikacji:
    2017
    Wydawca:
    Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
    Tematy:
    Pseudomonas aeruginosa
    biodegradation
    bioimmobilization
    encapsulation
    petroleum hydrocarbons
    Opis:
    The aim of the work was to elaborate new method of wool entrapping into silica alginate beads and checking of their adsorption properties. The purpose of the present study was also to determine water bioremediation efficiency using Pseudomonas aeruginosa cells immobilized into wool entrapped into silica-alginate beads. The results of study showed that obtained capsules are stable in saline water environment. Additionally neither silica adsorbers nor wool impacted negatively on viability of bacterial strains that were entrapped into these capsules. Moreover, it should be underline that 83,01% of adsorbed engine oil has been degraded by immobilized microorganisms after 3 months of biodegradation process. The results proved that properties and non-toxic character of these beads are suitable enough to use them in purification of water from petroleum hydrocarbons.
    Źródło:
    World Scientific News; 2017, 80; 256-267
    2392-2192
    Pojawia się w:
    World Scientific News
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    New Active Packaging Films Made from Gelatin Modified with Fungal Melanin
    Autorzy:
    Łopusiewicz, Łukasz
    Jędra, Filip
    Bartkowiak, Artur
    Powiązania:
    https://bibliotekanauki.pl/articles/1177694.pdf
    Data publikacji:
    2018
    Wydawca:
    Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
    Tematy:
    Agaricus bisporus
    antioxidant
    barrier properties
    gelatin
    mechanical properties
    melanin
    packaging
    Opis:
    Fungal melanin was used to prepare gelatin-based composite films. The films of various melanin concentrations (0.1%, 0.5% and 1% w/w) were prepared using a solution casting method. The mechanical, antioxidant, antimicrobial, water vapor, oxygen and UV-Vis barrier properties, as well as any available surface polyphenolics were studied. FT-IR and Raman spectroscopy studies were carried out to analyse the chemical composition of the resulting films. The hydrophobocity, solubility, colour response, optical properties and opacity were also determined. The results of this study showed that the modification of gelatin with fungal melanin had no influence on mechanical and water vapor barrier properties, oxygen barrier properties were improved. The UV-Vis barrier properties of modified films were significantly improved. Modified gelatin films showed good antioxidant activity but were inactive against microorganisms. The modification with melanin caused changes in colour values, decreasing lightness and increasing the redness and yellowness of the films. Based on the results of this study, fungal melanin has good potential to be utilised as a value-added modifier that can improve the properties of gelatin films.
    Źródło:
    World Scientific News; 2018, 101; 1-30
    2392-2192
    Pojawia się w:
    World Scientific News
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Microscopic fungi on Schoenoplectus lacustris in Plociczno and Plociowe lakes in Drawa National Park (northwest Poland)
    Autorzy:
    Mazurkiewicz-Zapałowicz, Kinga
    Rybińska, Aleksandra
    Łopusiewicz, Łukasz
    Powiązania:
    https://bibliotekanauki.pl/articles/952562.pdf
    Data publikacji:
    2017
    Wydawca:
    Polskie Towarzystwo Botaniczne
    Tematy:
    biodiversity
    microfungi
    schoenoplectus
    drawa national park
    Opis:
    In the period 2009–2011, the micromycetes causing symptoms on the leaves of Schoenoplectus lacustris (SL) from Płociczno and Płociowe lakes in Drawa National Park (DNP) were investigated. A total of 39 taxa of microscopic fungi and chromistan fungi were found. The taxonomic structure of the mycobiota was dominated by anamorphs of Ascomycota. In each year of the study, the SL fungal species richness was higher in Płociowe Lake than in Płociczno Lake. In all the years of study in both lakes, the fungal community on SL was composed of the following six dominant species: Alternaria alternata, Cladosporium herbarum, Fusarium incarnatum, F. sacchari, Gibberella avenacea, and Pleospora scirpicola. The species occurring exclusively in Płociczno Lake were Papulaspora immersa, Puccinia scirpi, and Trichothecium roseum, and those found only in Płociowe Lake were Acremoniella atra, Alternaria atra, Aspergillus niger, A. versicolor, Fusarium oxysporum, Gonatobotrys simplex, Massariosphaeria scirpina, Microascus brevicaulis, Penicillium chrysogenum, and Stagonospora aquatica. Only one confirmation of the occurrence of Puccinia scirpi and the lack of other specialized obligate parasites indicate the good health of SL in DNP. The facultative parasites, inter alia, Boeremia exigua, Chaetomium globosum, Fusarium culmorum, F. incarnatum, F. sacchari, Gibberella avenacea, and Stagonospora aquatica that are present on plants as part of the natural process of apoptosis may influence the health of SL.
    Źródło:
    Acta Mycologica; 2017, 52, 2
    0001-625X
    2353-074X
    Pojawia się w:
    Acta Mycologica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Lactobacillus casei cell immobilisation on mineral carriers for continuous lactic acid production
    Autorzy:
    Kowalska, Urszula
    Mizielińska, Małgorzata
    Łopusiewicz, Łukasz
    Bartkowiak, Artur
    Powiązania:
    https://bibliotekanauki.pl/articles/1178777.pdf
    Data publikacji:
    2017
    Wydawca:
    Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
    Tematy:
    Lactobacillus casei
    adsorption
    biofilm
    continuous production
    immobilisation
    lactic acid
    mineral carriers
    Opis:
    The first purpose of the study was to evaluate the influence of incubation parameters for efficiency of bacterial adhesion on surface of three solid, mineral porous carriers. Second aim of the study was the use of the immobilized Lactobacillus casei cells, in the production of lactic acid and selection, best carriers for continuous production using column bioreactor. The results showed that bacterial adhesion was different depending on the incubation conditions. In all tested samples, the highest efficiency of bacterial adhesion achieved after incubation at 30 °C without shaking which was related to bacterial viability. The results showed that, the highest efficiency of lactic acid production by free cells has been obtained at 30 °C after 48h – 110% (w/w) (lactic acid yield from consumed glucose). This results have been confirmed by further tests which have shown that in stationary culture, the highest production of lactic acid was reached by immobilised bacteria cells on pumice and kermiste after 48 hours at 30 °C.
    Źródło:
    World Scientific News; 2017, 90; 62-76
    2392-2192
    Pojawia się w:
    World Scientific News
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
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