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Wyświetlanie 1-15 z 41
Tytuł:
An improved method for RNA isolation from plants using commercial extraction kits
Autorzy:
Kalinowska, Elżbieta
Chodorska, Maria
Paduch-Cichal, Elżbieta
Mroczkowska, Karolina
Powiązania:
https://bibliotekanauki.pl/articles/1039717.pdf
Data publikacji:
2012
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
total RNA
secondary metabolites
Opis:
Isolation of RNA from plants rich in secondary metabolites using commercial kits often results in contaminated preparations which are not suitable for downstream applications. Although many specific protocols appropriate for plants with a high content of phenolics, anthocyanins and polysaccharides have been developed, these are often expensive, time consuming and not applicable to different types of tissues. This study presents a simple and efficient modification of RNA extraction from different types of tissues using two commercial reagent kits. By simple improvement, we routinely obtained high-quality RNA of the following plants: the blackcurrant bush, black chokeberry bush, pear tree, apricot tree, apple tree, hardy kiwi, tangerine tree, highbush blueberry and cranberry plant.
Źródło:
Acta Biochimica Polonica; 2012, 59, 3; 391-393
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Anethum graveolens L. in vitro cultures - a potential source of bioactive metabolites, phenolic acids and furanocoumarins
Autorzy:
Szopa, A.
Ekiert, H.
Powiązania:
https://bibliotekanauki.pl/articles/19449.pdf
Data publikacji:
2015
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
Anethum graveolens
in vitro culture
potential source
bioactive metabolite
phenolic acid
furanocoumarin
Apiaceae
plant growth regulator
secondary metabolite
high performance liquid chromatography
Źródło:
Acta Biologica Cracoviensia. Series Botanica; 2015, 57, 2
0001-5296
Pojawia się w:
Acta Biologica Cracoviensia. Series Botanica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Antibacterial activity of caffeine against plant pathogenic bacteria
Autorzy:
Sledz, Wojciech
Los, Emilia
Paczek, Agnieszka
Rischka, Jacek
Motyka, Agata
Zoledowska, Sabina
Piosik, Jacek
Lojkowska, Ewa
Powiązania:
https://bibliotekanauki.pl/articles/1039014.pdf
Data publikacji:
2015
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
antibacterial activity
plant secondary metabolites
Ralstonia solanacearum
Clavibacter michiganesis subsp. sepedonicus
Dickeya solani
Pectobacterium atrosepticum
Pseudomonas syringae pv. tomato and Xanthomonas campestris subsp. campestris
Opis:
The objective of the present study was to evaluate the antibacterial properties of a plant secondary metabolite - caffeine. Caffeine is present in over 100 plant species. Antibacterial activity of caffeine was examined against the following plant-pathogenic bacteria: Ralstonia solanacearum (Rsol), Clavibacter michiganesis subsp. sepedonicus (Cms), Dickeya solani (Dsol), Pectobacterium atrosepticum (Pba), Pectobacterium carotovorum subsp. carotovorum (Pcc), Pseudomonas syringae pv. tomato (Pst), and Xanthomonas campestris subsp. campestris (Xcc). MIC and MBC values ranged from 5 to 20 mM and from 43 to 100 mM, respectively. Caffeine increased the bacterial generation time of all tested species and caused changes in cell morphology. The influence of caffeine on the synthesis of DNA, RNA and proteins was investigated in cultures of plant pathogenic bacteria with labelled precursors: [3H]thymidine, [3H]uridine or 14C leucine, respectively. RNA biosynthesis was more affected than DNA or protein biosynthesis in bacterial cells treated with caffeine. Treatment of Pba with caffeine for 336 h did not induce resistance to this compound. Caffeine application reduced disease symptoms caused by Dsol on chicory leaves, potato slices, and whole potato tubers. The data presented indicate caffeine as a potential tool for the control of diseases caused by plant-pathogenic bacteria, especially under storage conditions.
Źródło:
Acta Biochimica Polonica; 2015, 62, 3; 605-612
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Application and properties of selected flavanones
Autorzy:
Błazińska, P.
Sykuła, A.
Powiązania:
https://bibliotekanauki.pl/articles/410506.pdf
Data publikacji:
2018
Wydawca:
Politechnika Łódzka. Wydawnictwo Politechniki Łódzkiej
Tematy:
flavanones
flavonoids
secondary plant metabolites
biological activity
flawanony
flawonoidy
wtórne metabolity roślinne
aktywność biologiczna
Opis:
Flavanones, secondary plant metabolites, are one of the main group of flavanoids. They are widely spread in nature in many plants. The large diversity of structural structure of flavanones and controlled methods of modifying their molecules have a huge impact on biological activity. The present review will summarize the current knowledge about occurrence, obtaining by chemical synthesis, application and bioactivity of flavanones. Also, they are received from specific chemical synthesis. Flavanones have a great biological activity. Derivatives of flavanone have many different properties such as anti-inflammatory, anticancer, antioxidant, antimicrobial or hepatoprotective activities. These natural polyphenolic compounds are used in cosmetology, pharmacy and medicine. The demand and usage on them increases.
Źródło:
Biotechnology and Food Science; 2018, 82, 1; 61-74
2084-0136
2299-6818
Pojawia się w:
Biotechnology and Food Science
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Artemisia species in vitro cultures for production of biologically active secondary metabolites
Autorzy:
Grech-Baran, M.
Pietrosiuk, A.
Powiązania:
https://bibliotekanauki.pl/articles/80902.pdf
Data publikacji:
2012
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
Artemisia
in vitro culture
hairy root
bioreactor
natural drug
arglabin
artemisinin
plant regeneration
callus culture
shoot culture
Źródło:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2012, 93, 4
0860-7796
Pojawia się w:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Betaxanthin Profiling in Beta vulgaris Leaves and Gymnocalycium mihanovichii Grafted Cacti: A Comprehensive Study
Autorzy:
Spórna-Kucab, Aneta
Tekieli, Anna
Knapek, Magdalena
Zużałek, Marta
Wybraniec, Sławomir
Powiązania:
https://bibliotekanauki.pl/articles/24024625.pdf
Data publikacji:
2023-12-05
Wydawca:
Instytut Rozrodu Zwierząt i Badań Żywności Polskiej Akademii Nauk w Olsztynie
Tematy:
beet
betalains
colorants
grafted cactus
LC-MS
secondary metabolites
Opis:
This study was focused on the identification and quantification of betaxanthins using high-performance liquid chromatography with a diode array detector coupled to electrospray ionisation mass spectrometry (HPLC-DAD-ESI-MS) in leaves of various cultivars of Beta vulgaris (beet) and Gymnocalycium mihanovichii grafted cacti. In G. mihanovichii grafted cacti, four betaxanthins, namely histidine-Bx, histamine-Bx, serine-Bx, and proline-Bx, were tentatively identified in the yellow, orange, pink, and red varieties, with contents ranging from 0.09 to 1.55 mg/kg fresh weight (FW). Betaxanthins were not detected in the green cactus. Histidine-Bx was the prevailing betaxanthin compound in the majority of cultivars. Fifteen betaxanthins were successfully identified in the leaves of five B. vulgaris cultivars (cv.): Snow Ball, Boldor, Cylindra, Rhubarb, and Round Dark Red. Leaves of yellow beet (cv. Boldor) had the highest total betaxanthin content (20.4 mg/kg FW), while white beet (cv. Snow Ball) had the lowest one (3.43 mg/kg FW). The leaves of red cultivars had comparable betaxanthin contents, ranging from 13.4 to 18.8 mg/kg FW, similary to the yellow cultivar, indicating their potential as valuable sources of betaxanthins. There was no single dominant betaxanthin in B. vulgaris leaves. The leaves of B. vulgaris were found to be a richer source of betaxanthin than the grafted cactus G. mihanovichii, which opens up new prospects for research into the potential utilization of these compounds in the industry.
Źródło:
Polish Journal of Food and Nutrition Sciences; 2023, 73, 4; 375-384
1230-0322
2083-6007
Pojawia się w:
Polish Journal of Food and Nutrition Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Bioinformatic insight into Portulaca oleracea L. (Purslane) of Bulgarian and Greek origin
Autorzy:
Balabanova, V.
Hristov, I.
Zheleva-Dimitrova, D.
Sugareva, P.
Lozanov, V.
Gevrenova, R.
Powiązania:
https://bibliotekanauki.pl/articles/2117810.pdf
Data publikacji:
2020
Wydawca:
Polska Akademia Nauk. Czasopisma i Monografie PAN
Tematy:
Portulaca oleracea
LC–HRAMS
secondary metabolites
descriptive analysis
multivariate statistics
Opis:
Portulaca oleracea L. (Portulacaceae) is used as functional food and its nutritional and therapeutic properties are related to the high levels of organic and fatty acids, polyphenols, polysaccharides and cyclo-dopa amides. This study presents a strategy based on liquid chromatography – high resolution accurate mass spectrometry method (LC – HRAMS) and bioinformatic methods to analyze 33 purslane accessions originating from 11 floristic regions in Bulgaria together with 5 accessions of Greek provenance. Extracts were obtained by microwave extraction. Based on the LC-MS metabolic “fingerprints” of assayed samples, a purslane metabolic database was developed. LC-MS data were proceeded with Software application Compound Discover 2.0 (Thermo Fischer Sci., USA). Principal Component Analysis (PCA) combined with both descriptive and differential analyses were used to find marker metabolites to distinguish different geographical regions. The differential analysis of the Bulgarian and Greek samples allowed the identification of 50 marker metabolites. Based on accurate masses, retention times, fragmentation patterns in MS/MS, comparison with commercial standards and literature data, these secondary metabolites were identified after detailed analysis of Volcano-plots. For the first time, 29 compounds are reported. The identified compounds were used to perform a study of the biosynthetic pathways of purslane secondary metabolites using Kyoto Encyclopedia of Genes and Genomes (KEGG) software platform. The statistical treatments identified marker compounds that can be used to distinguish the origin of accession set. Combining LC-MS data with multivariate statistical analysis was shown to be effective in studying the purslane metabolites, allowing for integration of chemistry with geographic origin.
Źródło:
Acta Biologica Cracoviensia. Series Botanica; 2020, 62, 1; 7-21
0001-5296
Pojawia się w:
Acta Biologica Cracoviensia. Series Botanica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Biosynteza metabolitów wtórnych przez Aspergillus terreus
Biosynthesis of secondary metabolites by Aspergillus terreus
Autorzy:
Bizukojć, M.
Ledakowicz, S.
Powiązania:
https://bibliotekanauki.pl/articles/2070446.pdf
Data publikacji:
2009
Wydawca:
Stowarzyszenie Inżynierów i Techników Mechaników Polskich
Tematy:
Aspergillus terreus
lowastatyna
(+)-geodyna
lovastatin
(+)-geodin
Opis:
W pracy przedstawiono biosyntezę metabolitów wtórnych Aspergillus terreus na przykładzie lowastatyny i (+)-geodyny, w ujęciu biochemiczno-bioinformatycznym, fizjologicznym, kinetycznym i morfologicznym.
Biosynthesis of Aspergillus terreus secondary metabolites lovastatin and (+)-geodin as the examples was presented. Biochemical and bioin-formatics, physiological, kinetic and morphological aspects of this process were discussed.
Źródło:
Inżynieria i Aparatura Chemiczna; 2009, 3; 23-25
0368-0827
Pojawia się w:
Inżynieria i Aparatura Chemiczna
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Biosynthesis of defensive secondary metabolites of spice plants in answer to heavy metal stress condition
Autorzy:
Szczodrowska, A.
Kulbat, K.
Leszczynska, J.
Powiązania:
https://bibliotekanauki.pl/articles/80817.pdf
Data publikacji:
2013
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
conference
biosynthesis
secondary metabolite
spice plant
heavy metal
stress condition
polyphenolic compound
pathogenesis-related protein
antimicrobial activity
protein
Źródło:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2013, 94, 3
0860-7796
Pojawia się w:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Callus cultures of Harpagophytum procumbens (Burch.) DC. ex Meisn. – production of secondary metabolites and antioxidant activity
Autorzy:
Grabkowska, R.
Matkowski, A.
Grzegorczyk-Karolak, I.
Wysokinska, H.
Powiązania:
https://bibliotekanauki.pl/articles/951258.pdf
Data publikacji:
2015
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
conference
callus culture
Harpagophytum procumbens
antioxidant activity
secondary metabolite
root tuber
analgesic effect
antiinflammatory effect
rheumatic disease
osteoarthritis
Źródło:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2015, 96, 1
0860-7796
Pojawia się w:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Chemical diversity of silverweed (Potentilla anserina L.) growing at the edges of arable fields.
Autorzy:
Angielczyk, Mirosław
Bączek, Katarzyna
Geszprych, Anna
Przybył, Jarosław L.
Węglarz, Zenon
Powiązania:
https://bibliotekanauki.pl/articles/2199570.pdf
Data publikacji:
2010-06-01
Wydawca:
Instytut Hodowli i Aklimatyzacji Roślin
Tematy:
catechins
flavonoids
phenolic acids
populations
secondary metabolites
silverweed herb
tannins
Opis:
The medicinal usage of silverweed herb (Anserinae herba) is primarily related to the presence of phenolic compounds. Three populations of silverweed (Potentilla anserina L.) occurring at the edges of arable fields in Podlasie area (north-eastern Poland) were compared in respect of the content and composition of phenolics. Herb collected from the investigated populations differed in the content of determined compounds. The tannin content was very low (0.81-0.91%). The total flavonoid content ranged from 0.48 to 0.60%. Phenolic acid content was much higher (1.38-2.26%). Three flavan-3-ols: (+)-catechin, (-)-epicatechin, and (-)-epigallocatechin, four flavonol glycosides: rutoside, quercetin-3-O-glucoside, isorhamnetin-3-O-glucoside, and kaempferol-3-O-glucoside, as well as one phenolic acid (ellagic acid) were identified as a_result of HPLC analysis of methanolic extracts from the investigated raw materials. The most considerable differences between populations concerned the content of (+)-catechin (87.0-199.3 mg × 100 g-1), (-)-epigallocatechin (141.9-290.6 mg × 100 g-1), and rutoside (192.3-386.0 mg × 100 g-1) in herb...
Źródło:
Plant Breeding and Seed Science; 2010, 61; 41-46
1429-3862
2083-599X
Pojawia się w:
Plant Breeding and Seed Science
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Chromatographic and mass spectrometric analysis of secondary metabolites of Deschampsia antarctica from Galindez Island, Argentine Islands
Autorzy:
Ivannikov, Roman
Anishchenko, Viktor
Kuzema, Pavlo
Stavinskaya, Oksana
Laguta, Iryna
Poronnik, Oksana
Parnikoza, Ivan
Powiązania:
https://bibliotekanauki.pl/articles/16538469.pdf
Data publikacji:
2022
Wydawca:
Polska Akademia Nauk. Czasopisma i Monografie PAN
Tematy:
West Antarctic
antarctic hairgrass
polyphenolic antioxidants
flavonoids
luteolin derivatives
Opis:
The aim of this work was to study the polyphenolic composition of Deschampsia antarctica È. Desv. plants grown at natural conditions on different locations on the Galindez Island, Argentine Islands, the maritime Antarctic. The plants were collected during the summer season of the 26th Ukrainian Antarctic Expedition (2020–2022). The extracts of 21 plants were obtained and the composition of the extracts was analyzed by means of high-performance liquid chromatography and matrix-assisted laser desorption/ionization mass spectrometry. The antioxidant properties of the extracts were characterized using the DPPH (2,2-diphenyl-1-picrylhydrazyl) test. The extracts were found to contain large amount of polyphenolic compounds, with flavonoids and phenolic acids, as well as their derivatives, being the most common classes of the phenols. Using the HPLC data the content of various phenols in the plants was systematic studied. It has been found that in all plants the most abundant phenols are flavonoids/flavonoid derivatives (on average about 75% of total mass of phenols). Among the flavonoids, luteolin derivatives predominate (86–94% of the total mass of flavonoids), and, among luteolin derivatives, the main compounds are orientin, orientin 2"- O-β-arabinopyranoside and isoswertiajaponin 2"- O-β-arabinopyranoside (67–83% of the total mass of luteolin derivatives). It has been also found that all the extracts possess the high activity in inhibition of DPPH radicals and that the antioxidant activity of the extracts correlates with total content of phenols in the samples. Thus, Deschampsia antarctica É. Desv. plants are a valuable source of natural phenolic antioxidants, and the most common antioxidants in the extracts are orientin, orientin 2"- O-β-arabinopyranoside and isoswertiajaponin 2"- O-β-arabinopyranoside.
Źródło:
Polish Polar Research; 2022, 43, 4; 341-362
0138-0338
2081-8262
Pojawia się w:
Polish Polar Research
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Comparative analysis of secondary metabolites contents in Fragaria vesca L. fruits
Autorzy:
Najda, A.
Dyduch-Sieminska, M.
Dyduch, J.
Gantner, M.
Powiązania:
https://bibliotekanauki.pl/articles/50720.pdf
Data publikacji:
2014
Wydawca:
Instytut Medycyny Wsi
Źródło:
Annals of Agricultural and Environmental Medicine; 2014, 21, 2
1232-1966
Pojawia się w:
Annals of Agricultural and Environmental Medicine
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Composition, biological properties and therapeutic effects of lavender (Lavandula angustifolia L.). A review
Lawenda wąskolistna (Lavandula angustifolia L.) - lecznicza siła natury. Przegląd
Autorzy:
Prusinowska, R.
Smigielski, K.B.
Powiązania:
https://bibliotekanauki.pl/articles/950560.pdf
Data publikacji:
2014
Wydawca:
Instytut Włókien Naturalnych i Roślin Zielarskich
Tematy:
lavender
lavandula angustifolia
herbs
essential oils
phytotherapy
secondary plant
metabolites
Opis:
Lavender (Lavandula angustifolia) is a shrub of the family Lamiaceae, native to the Mediterranean region. The material used for herbal purposes includes lavender flowers (Lavandula flores) containing essential oil (3%), anthocyanins, phytosterols, sugars, minerals, and tannins. The qualitative and quantitative composition of the essential oil of lavender is variable and depends on genotype, growing location, climatic conditions, propagation, and morphological features. The essential oil contains over 300 chemical compounds. The dominant components are linalool, linalyl acetate, terpinen-4-ol, acetate lavandulol, ocimene, and cineole. Lavender essential oil has good antioxidant and antimicrobial activities and a significant positive effect on the digestive and nervous systems. Lavender extract prevents dementia and may inhibit the growth of cancer cells, while lavender hydrolate is recommended for the treatment of skin problems and burns.
Lawenda wąskolistna (Lavandula angustifolia) to krzewiasta roślina z rodziny jasnotowatych (Lamiaceae), pochodząca z terenów basenu Morza Śródziemnego. Surowcem zielarskim są kwiaty lawendy (Lavandula flores), zawierające olejek eteryczny (do 3%), antocyjany, fitosterole, cukry, związki mineralne i garbniki. Skład jakościowy i ilościowy olejku eterycznego z lawendy jest zmienny i zależy od genotypu, miejsca uprawy, warunków klimatycznych, sposobu rozmnażania i cech morfologicznych. W olejku eterycznym wstępuje ponad 300 związków chemicznych, składnikami dominującymi są linalol, octan linalilu, terpinen-4-ol, octan lawandulolu, ocymen oraz cyneol. Olejek eteryczny charakteryzuje dobra aktywność przeciwdrobnoustrojowa i antyoksydacyjna oraz wyraźny pozytywny wpływ na układ trawienny i nerwowy. Ekstrakt z lawendy przeciwdziałała otępieniu oraz może wpływać hamująco na rozwój komórek rakowych, zaś hydrolat lawendowy zalecany jest w leczeniu problemów skórnych i poparzeń.
Źródło:
Herba Polonica; 2014, 60, 2
0018-0599
Pojawia się w:
Herba Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Discoloured seeds of amaranth plant infected by Alternaria alternata: physiological, histopathological alterations and fungal secondary metabolites associated or registered
Autorzy:
Noelting, M.C.
Sisterna, M.
Lovisolo, M.
Molla-Kralj, A.
Lori, G.
Sandoval, M.C.
Sulyok, M.
Molina, M.C.
Powiązania:
https://bibliotekanauki.pl/articles/65891.pdf
Data publikacji:
2016
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
seed
pathology
amaranth
plant infection
Alternaria alternata
physiological alteration
histopathological alteration
fungal metabolite
secondary metabolite
hypertrophy
Opis:
In the present study the aspects of discolouration that could influence both the production and consumption of amaranth were analyzed with the objectives to identify the presence of Alternaria alternata on seeds, to analyze possible changes in the anatomy of seed tissues and to detect the presence of fungal secondary metabolites. Component plating, histopathological and mycological analyses on discoloured seeds allowed i) location of propagules of A. alternata in all seminal components; ii) observation of hypertrophies in perisperm and embryo and iii) determination of several fungal secondary metabolites, mainly high concentrations of tenuazonic acid. To our knowledge, the information presented in this paper, related to physiological, histopathological changes and fungal secondary metabolites on discoloured seeds of (Amaranthus mantegazzianus syn. A. caudatus subsp. mantegazzianus (Pass) Hanelt affected by A. alternata, is the first worldwide record.
Źródło:
Journal of Plant Protection Research; 2016, 56, 3
1427-4345
Pojawia się w:
Journal of Plant Protection Research
Dostawca treści:
Biblioteka Nauki
Artykuł
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