Wszystkie pola: in vitro analysis - Katalog OPAC zbiorów

Skocz do pozycji: 1.

Tytuł:: The in vitro analysis of scaffolds with glycosaminoglycans addition
Autorzy:: Kaczmarek, B.
Sionkowska, A.
Łukowicz, K.
Osyczka, A. M.
Unrod, V.
Powiązania:: https://bibliotekanauki.pl/articles/283839.pdf
Data publikacji:: 2018
Wydawca:: Akademia Górniczo-Hutnicza im. Stanisława Staszica w Krakowie. Polskie Towarzystwo Biominerałów
Tematy:: scaffolds
polysaccharides
collagen
Źródło:: Engineering of Biomaterials; 2018, 21, 148; 28
1429-7248
Pojawia się w:: Engineering of Biomaterials
Dostawca treści:: Biblioteka Nauki

Artykuł

Zmień widok

na półce

Skocz do pozycji: 2.

Tytuł:: The in vitro analysis of thin films based on chitosan/tannic ACID
Autorzy:: Kaczmarek, Beata
Miłek, Oliwia
Nadolna, Kinga
Owczarek, Agata
Kleszczyński, Konrad
Osyczka, Anna M.
Powiązania:: https://bibliotekanauki.pl/articles/284432.pdf
Data publikacji:: 2019
Wydawca:: Akademia Górniczo-Hutnicza im. Stanisława Staszica w Krakowie. Polskie Towarzystwo Biominerałów
Źródło:: Engineering of Biomaterials; 2019, 22, no.153; 34
1429-7248
Pojawia się w:: Engineering of Biomaterials
Dostawca treści:: Biblioteka Nauki

Artykuł

Zmień widok

na półce

Skocz do pozycji: 3.

Tytuł:: In Vitro Analysis of AHPlus and MM-Seal by ESR and Thermoanalytical Methods
Autorzy:: Ceylan, Y.
Usta, A.
Usta, K.
Kont Cobankara, F.
Yildirim, C.
Birey, M.
Powiązania:: https://bibliotekanauki.pl/articles/1402525.pdf
Data publikacji:: 2015-08
Wydawca:: Polska Akademia Nauk. Instytut Fizyki PAN
Tematy:: 87.66.Uv
36.20.Kd
39.30.+w
33.35.+r
Opis:: The free radicals and their concentration in resin materials may impact biocompatibility and polymerization properties of dental materials. The aim of this study was to determine whether there are free radicals and to obtain useful information about thermal stability of materials using electron spin resonance (ESR) spectroscopy, TGA (Thermogravimetry Analysis) and DTA (Differential Thermal Analysis) methods. Epoxy resin-based sealers AHPlus and MM-Seal samples, freshly mixed and set, were prepared to be analysed with ESR and thermal methods. The free radicals were found in dental materials. As radical concentration in AHPlus have changed very slowly, the concentration in MM-Seal have decreased dramatically. Also, MM-Seal was found to decompose in three steps with the increasing temperature, while decomposition of the AHPlus occurred in two steps.
Źródło:: Acta Physica Polonica A; 2015, 128, 2B; B-479-B-482
0587-4246
1898-794X
Pojawia się w:: Acta Physica Polonica A
Dostawca treści:: Biblioteka Nauki

Artykuł

Zmień widok

na półce

Skocz do pozycji: 4.

Tytuł:: Evaluation of the effects of photostimulation on freeze-thawed bull sperm cells in terms of reproductive potential
Autorzy:: Ömür, A.D.
Powiązania:: https://bibliotekanauki.pl/articles/16539165.pdf
Data publikacji:: 2022
Wydawca:: Polska Akademia Nauk. Czasopisma i Monografie PAN
Tematy:: in vitro analysis
laser
LED (Light Emitting Diode)
photostimulation
sperm cell
Opis:: The aim of this study was to determine the time-dependent effectiveness of photo-stimulation against bovine sperm cells using a multi-wavelength LED (Light Emitting Diode). Spermatological parameters were evaluated for viability, acrosome structure and motility. In addition, the effect of photo-stimulation on frozen-thawed sperm cells subjected to in vitro capacitation was evaluated in terms of changes in mitochondrial membrane potential. The study consisted of two separate experiments and a total of 32 sperm samples obtained from separate bulls were used. All sperm samples were obtained from Holstein bulls using an artificial vagina. Semen was diluted to a final concentration of 92 x 106 spermatozoa per ml in 0.25 ml straws. The sperm cells were frozen using the conventional method. Straws were kept in a 37°C water bath for 20 seconds and diluted 1:4 in phosphate buffered saline (PBS) to eliminate the potentially deleterious effect of glycerol, the main permeable cryoprotectant in the freezing medium for bull sperm. This dilution also helped in the evaluation of sperm quality parameters. In the first experiment, whereas the 15-10-15 showed no differences with the control, other treatments such as 10-10-10, 5-5-5, and 3-1-3 exhibited significantly higher percentages of viable spermatozoa at 24h. The results obtained for acrosome integrity were pretty much similar to those observed in the sperm viability assessment. In effect, while the treatment consisting of 15-10-15 had no positive effects, shorter treatments exerted a much more positive effect. The percentages of acrosome-intact spermatozoa in 2-1-2 and 1-1-1 were significantly higher than those obtained in the control. The significant differences in mitochondrial membrane potential were observed at 0, 2, 4 and 24h post-photo-stimulation in all treatments, except 15-10-15. The highest increase in the percentage of spermatozoa exhibiting high mitochondrial membrane potential was found in 10-10-10, 5-5-5 and 3-1-3 treatments. With regard to total and progressive motility, whereas 10-10-10 was the best regime, 5-5-5 and 3-1-3 treatments also had a positive effect. However, 15-10-15 appeared to have a stimulating effect upon progressive motility at 2h and 4h but later declined and showed no significant differences with regard to the control at 24h. In the second experiment, not immediately after thawing but after having been kept at room temperature for up to 24h, it was observed that there was no statistical difference in terms of viability, acrosome integrity and total/progressive motility between photostimulation and the control group. This indicates that photo- stimulation is less able to exert a beneficial effect when post-thawed sperm are not immediately stimulated. As a result it was determined that photo-stimulation at a pattern of 10-10-10, 5-5-5, 3-1-3 and, to a lesser extent 2-1-2, increases the resilience of frozen-thawed bull sperm when applied upon thawing.
Źródło:: Polish Journal of Veterinary Sciences; 2022, 25, 2; 249-259
1505-1773
Pojawia się w:: Polish Journal of Veterinary Sciences
Dostawca treści:: Biblioteka Nauki

Artykuł

Zmień widok

na półce

Skocz do pozycji: 5.

Tytuł:: In vitro expression analysis of R68G and R68S mutations in phenylalanine hydroxylase gene.
Autorzy:: Żekanowski, Cezary
Perez, Belen
Desviat, Lourdes
Wiszniewski, Wojciech
Ugarte, Magdalena
Powiązania:: https://bibliotekanauki.pl/articles/1044360.pdf
Data publikacji:: 2000
Wydawca:: Polskie Towarzystwo Biochemiczne
Tematy:: phenylalanine hydroxylase
expression analysis
mutation
hyperphenylalaninemia
Opis:: Phenylketonuria (PKU), an autosomal recessive disorder caused be a deficiency of hepatic phenylalanine hydroxylase (PAH), is clinically very heterogeneous. At the molecular level, more than 400 mutations in the PAH gene are known to date, which in different genotype combinations could account for biochemical and clinical variability of symptoms. In vitro expression studies on R68G and R68S mutations causing mild phenylketonuria are presented.
Źródło:: Acta Biochimica Polonica; 2000, 47, 2; 365-369
0001-527X
Pojawia się w:: Acta Biochimica Polonica
Dostawca treści:: Biblioteka Nauki

Artykuł

Zmień widok

na półce

Skocz do pozycji: 6.

Tytuł:: PEGYLATED GEMCITABINE AND METHOTREXATE: IN VITRO CYTOTOXICITY ANALYSIS AND IN VIVO DRUG RELEASE PROFILING TO ASSESS THEIR ENHANCED BLOOD CIRCULATION
Autorzy:: Ahmed, Mahmood
Powiązania:: https://bibliotekanauki.pl/articles/895274.pdf
Data publikacji:: 2019-08-30
Wydawca:: Polskie Towarzystwo Farmaceutyczne
Tematy:: drug delivery
anti-proliferation
Pegylation
spheroids
Opis:: In the present study, four new pegylated drugs were synthesized to investigate the anticancer activity. All the compounds were characterized on the basis of FTIR and 1HNMR. The new compounds were evaluated against HCT116 cell line by CellTiter-Glo® 3D cell viability assay. The sustained release pattern of newly synthesized compounds were also evaluated, slow released lasts for 240 h which was confirmed by in vivo and in vitro drug release profile. The sustained and controlled release behavior of pegylated drugs over the extended period of time showed a linear profile and this release pattern was not limited to diffusion process instead supporting chemically controlled release pattern which was dependent only on how fast the compound hydrolyzed. The newly designed pegylated drugs showed almost same killing effect against the cancer cells as the free drugs. The compounds with lower cLogP value showed more antiproliferative (lower IC50) effect due their higher cell permeability.
Źródło:: Acta Poloniae Pharmaceutica - Drug Research; 2019, 76, 4; 653-659
0001-6837
2353-5288
Pojawia się w:: Acta Poloniae Pharmaceutica - Drug Research
Dostawca treści:: Biblioteka Nauki

Artykuł

Zmień widok

na półce

Skocz do pozycji: 7.

Tytuł:: Chemical composition and cytotoxic activity of the polysaccharide fraction in Sarcodon imbricatus (Basidiomycota)
Skład chemiczny i aktywność cytotoksyczna frakcji polisacharydowych Sarcodon imbricatus (Basidiomycota)
Autorzy:: Sulkowska-Ziaja, K.
Muszynska, B.
Ekiert, H.
Powiązania:: https://bibliotekanauki.pl/articles/67690.pdf
Data publikacji:: 2012
Wydawca:: Polskie Towarzystwo Botaniczne
Tematy:: chemical composition
cytotoxic activity
polysaccharide fraction
Sarcodon imbricatus
Basidiomycota
cytotoxicity
breast cancer
mycelium
in vitro culture
qualitative analysis
Opis:: The aim of the study was chemical analysis of polysaccharide fractions from sporocarps of Sarcodon imbricatus collected in natural sites and from the mycelium of in vitro cultures. Three polysaccharide fractions (FOI, FOII, FOIII) were isolated from sporocarps and two (FKI, FKII) from in vitro cultures. Qualitative analysis by HPLC method showed that they are composed of galactose and fucose (FOI, FKI) or glucose and fucose (FOII, FKII). FOIII fraction of the sporocarps consisted of glucose only. Molecular weights of isolated fractions ranged from 3.8 to 16.3 kDa for fractions from the sporocarps and from 5.8 to 14.7 kDa for that ones isolated from in vitro culture. The total percentage of sugar content for all fractions ranged from 97.8% to 99.1%. The percentage of uronic acids contents in acidic fractions was 2.6% and 2.7% for the FOI and FKI respectively. The work included also an assessment of cytotoxic activity of polysaccharide fractions in relation to tumor cell lines of human breast cancer MCV-7. FOI polysaccharide fraction of the sporocarps inhibited the growth of cancer cells in 50% compared to the control at a concentration of 0.0125%, while the polysaccharide fraction FKI from in vitro cultures inhibited cell growth in a concentration of 0.016%.
Przedstawiciele gromady Basidiomycota – której reprezentantem jest Sarcodon imbricatus (L.: Fr.) P. Karst., są ważnym źródłem związków aktywnych biologicznie. W ich owocnikach wykryto m.in. związki o działaniu przeciwwirusowym, przeciwbakteryjnym, fungistatycznym, a także przeciwnowotworowym. Najlepiej poznanymi metabolitami grzybowymi są polisacharydy. Coraz częściej podejmowane są badania nad składem chemicznym nie tylko owocników, ale także grzybni pozyskanej z kultur in vitro. W badaniach z tego zakresu dowiedziono istnienia jakościowych i ilościowych różnic w produkcji niektórych grup związków chemicznych przez owocniki i grzybnię z kultur in vitro. Obiektem przeprowadzonych badań były owocniki Sarcodon imbricatus zebrane na terenie lasów świerkowych w roku 2008. Z warstwy hymenialnej owocnika wyprowadzono kultury in vitro, które prowadzono, jako płynne, wytrząsane. W ramach przeprowadzonych badań wyizolowano 3 frakcje polisacharydowe (FOI, FOII, FOIII) z owocników i 2 (FKI, FKII) z mycelium z kultur in vitro. Analiza jakościowa z wykorzystaniem metody HPLC wykazała, że składają się one z galaktozy i fruktozy (FOI, FKI) oraz glukozy i fruktozy (FOII, FKII). Frakcja FOIII z owocników składała się wyłącznie z glukozy. Oznaczono masy cząsteczkowe wyizolowanych frakcji. Wynosiły one od 3.8 do 16.3 kDa dla frakcji z owocników i od 5.8 do 14.7 kDa dla frakcji z kultur in vitro. Całkowita procentowa zawartość cukrów dla wszystkich frakcji mieściła się w przedziale od 97.8% do 99.1%, natomiast procentowa zawartość kwasów uronowych we frakcjach o charakterze kwaśnym wynosiła 2.6% dla frakcji FOI z owocników i 2.7% dla frakcji FKI z kultur in vitro. Druga część pracy obejmowała ocenę aktywności cytotoksycznej wybranych frakcji polisacharydowych w stosunku do linii komórek nowotworowych ludzkich raka sutka MCV-7 w teście in vitro. Frakcje polisacharydowe FOI i FKI wykazywały działanie hamujące na komórki nowotworowe. Frakcja polisacharydowa FOI z owocników hamowała wzrost komórek nowotworowych w 50% w stosunku do próby kontrolnej w stężeniu 0.0125%, natomiast frakcja polisacharydowa FKI z kultur in vitro hamowała wzrost komórek w stężeniu 0.016%.
Źródło:: Acta Mycologica; 2012, 47, 1
0001-625X
2353-074X
Pojawia się w:: Acta Mycologica
Dostawca treści:: Biblioteka Nauki

Artykuł

Zmień widok

na półce

Skocz do pozycji: 8.

Tytuł:: Analysis of the bacterial biofilm formation in different models of the in vitro culture
Autorzy:: Bogut, Agnieszka
Magryś, Agnieszka
Powiązania:: https://bibliotekanauki.pl/articles/2040254.pdf
Data publikacji:: 2021-03-30
Wydawca:: Uniwersytet Rzeszowski. Wydawnictwo Uniwersytetu Rzeszowskiego
Tematy:: in vitro biofilm
microtiter plate assay
RPMI 1640
tryptic soy broth
Opis:: Introduction. Microtiter plate assay (MPA) remains one of workhorses of in vitro biofilm research but it requires optimization of experimental conditions to fulfill the biofilm formation requirements of different bacterial pathogens. Aim. The aim was to determine the effect of TSB and RPMI1640 culture media and selected culture variables (O2 vs. 5% CO2 , extended incubation time) on the biofilm production by bacteria commonly involved in biofilm-related infections: Enterococcus faecalis (EF), Escherichia coli (EC), Staphylococcus aureus (SA), Pseudomonas aeruginosa (PA), Klebsiella pneumoniae (KP). Material and methods. The investigation was performed using the MPA with crystal violet. Results. Statistically significant (p<0.05) increase in biofilm production between 24h and 72h time points was observed for EF (TSB o2, RPMIo2 and RPMIco2 ), EC (TSBo2 ) , SA (TSBo2 , TSBco2 ), KP (TSBo2 , TSBco2 ), PA (RPMIco2, TSBco2 ). The TSB caused a significantly greater stimulation of biofilm production compared to RPM1640. It outcompeted RPMI1640 irrespective of the atmospheric conditions for SA and KP and under aerobic conditions for EF. Conclusion. Although the TSB provided the most optimal conditions for biofilm production, the process was influenced by the strain type, atmospheric conditions and period of cultivation which limits the ability to design a single universal model of the in vitro biofilm investigation.
Źródło:: European Journal of Clinical and Experimental Medicine; 2021, 1; 40-45
2544-2406
2544-1361
Pojawia się w:: European Journal of Clinical and Experimental Medicine
Dostawca treści:: Biblioteka Nauki

Artykuł

Zmień widok

na półce

Skocz do pozycji: 9.

Tytuł:: Isobolographic in vitro interactions of fluconazole with citrus essential oils against Cladosporium cladosporioides
Autorzy:: Wroblewska-Luczka, P.
Powiązania:: https://bibliotekanauki.pl/articles/2098240.pdf
Data publikacji:: 2020
Wydawca:: Instytut Medycyny Wsi
Tematy:: fluconazole
essential oil
in vitro interaction
isobolographic analysis
Cladosporium cladosporioides
Opis:: Introduction. Cladosporium is one of the most abundant genera of environmental fungi worldwide and a very common respiratory allergen. To-date, many C. cladosporoides infections have been identified. Risk factors for C. cladosporioides infection are primarily injuries, metabolic disorders, organ transplantation and autoimmune diseases, among others. Objective. The aim of the study was to assess the type of pharmacodynamic interactions between fluconazole and some selected essential oils: orange, mandarin, lemon and grapefruit, in an in vitro study against C. cladosporioides. Materials and method. Experiments were carried out using the plate cultivation method. Fluconazole was tested against C. cladosporioides at concentrations ranging from 0.05–3.3 mg / ml, and the activity of essential oils added to PDA medium at concentrations ranging from 1–30%. The dose-effect curves for the collected results were determined with by the log- probit method. Isobolographic analysis of the results allowed determining the type of interactions between fluconazole and the tested essential oils. Results. Lemon essential oil was the most active, and in a concentration of 1% it inhibited the growth of C. cladosporioides by 21%. Isobolographic analysis showed that the combination of fluconazole with orange and grapefruit essential oil had an additive interaction, and with mandarin and lemon – an additive interaction with a tendency to synergy in the plate culture test for C. cladosporioides. Conclusions. The use of isobolographic analysis can contribute to the introduction of natural substances with the desired activities into the pharmacotherapy of many infections and diseases. The use of natural substances can also help to reduce the number of side-effects caused by conventional and standard therapies.
Źródło:: Journal of Pre-Clinical and Clinical Research; 2021, 15, 1; 15-19
1898-2395
Pojawia się w:: Journal of Pre-Clinical and Clinical Research
Dostawca treści:: Biblioteka Nauki

Artykuł

Zmień widok

na półce

Skocz do pozycji: 10.

Tytuł:: Analysis of microbiologically stimulated biomass of Salix viminalis L. in the presence of CD2plus under in vitro conditions - implications for phytoremediation
Autorzy:: Zloch, M.
Tyburski, J.
Hrynkiewicz, K.
Powiązania:: https://bibliotekanauki.pl/articles/19478.pdf
Data publikacji:: 2015
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: biomass analysis
Salix viminalis
willow
in vitro condition
phytoremediation
cadmium
hydrogen peroxide
Źródło:: Acta Biologica Cracoviensia. Series Botanica; 2015, 57, 2
0001-5296
Pojawia się w:: Acta Biologica Cracoviensia. Series Botanica
Dostawca treści:: Biblioteka Nauki

Artykuł

Zmień widok

na półce

Informacja

Wyszukujesz frazę "in vitro analysis" wg kryterium: Wszystkie pola

Źródło danych

Dostawca treści

Kolekcja

Rok wydania

Wydawca

Temat

Autor

Typ dokumentu

Język