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Tytuł:
Grupa Wyszehradzka urzędów probierczych. Urząd Probierczy w Budapeszcie
Visegrad Group of Assay Offices. Assay Office in Budapest
Autorzy:
Ulaczyk, Maria Magdalena
Powiązania:
https://bibliotekanauki.pl/articles/2200076.pdf
Data publikacji:
2021
Wydawca:
Główny Urząd Miar
Tematy:
probiernictwo
Grupa Wyszehradzka
Urząd Probierczy w Budapeszcie
assay
Visegrad Group
Assay Office in Budapest
Opis:
Artykuł informuje o współpracy urzędów probierczych, prowadzonej w ramach Grupy Wyszehradzkiej oraz przedstawia, wizytowany niedawno przez pracowników OUP w Warszawie, Urząd Probierczy w Budapeszcie. W artykule wskazano jego specyfikę i odrębności w odniesieniu do polskich urzędów probierczych.
The article informs about the cooperation between assay offices carried out within the Visegrad Group and presents the recently visited by employees of Warsaw Assay Office, Assay Office in Budapest. The article shows its specific and distinctiveness, in comparison to polish assay offices.
Źródło:
Metrologia i Probiernictwo : biuletyn Głównego Urzędu Miar; 2021, 2 (27); 49--58
2300-8806
Pojawia się w:
Metrologia i Probiernictwo : biuletyn Głównego Urzędu Miar
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Identification of sterile wild oat (Avena sterilis L.) resistance to acetolactate synthase (ALS)-inhibiting herbicides using different assay techniques
Autorzy:
Abdurruhman, A.M.
Uygur, S.
Mathiassen, S.K.
Uygur, N.
Powiązania:
https://bibliotekanauki.pl/articles/2082753.pdf
Data publikacji:
2020
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
acetolactate synthase
agar-based assay
Avena sterilis
Petri-dish assay
whole-plant assay
Opis:
Different techniques have been devised to detect herbicide resistance in weeds, and the over- all aim from this study was to compare four different assay techniques for evaluating acetol- actate synthase (ALS)-inhibiting herbicide resistance in sterile wild oat (Avena sterilis L.). A resistant sterile wild oat population (R) was collected from the wheat field in Kozan, Adana province, Turkey. The susceptible (S) population was collected from the border of the same field. Effects of different doses of mesosulfuron-methyl + iodosulfuron-methyl- -sodium and pyroxsulam + cloquintocet-mexyl were assessed in agar based (seed and seed- ling) assay, Petri dish with seeds, and whole plant pot assay. In the agar based assays, the level of resistance was evaluated by measuring coleoptile and hypocotyl lengths, and sur- vival of seedlings. Plant height and shoot dry weight were measured in the Petri dish and whole plant pot assays, respectively. Results from the dose response analyses showed that both the R and S populations were extremely sensitive to mesosulfuron-methyl + iodosul- furon in the seedling bioassay. The resistance indices (RI’s) of the R biotype treated with mesosulfuron-methyl + iodosulfuron in the agar based seed, Petri dish, and whole plant assays were 2.29, 2.63 and 4.18, respectively. The resistance indices of the R biotype treated with pyroxsulam + cloquintocet-mexyl was 3.41, 5.05 and 2.82 in the agar based seed, Petri dish, and whole plant pot assays, respectively. The agar based seed assays and Petri dish as- say provided feasible, accurate, rapid, and cost effective opportunities to identify resistance in sterile wild oat.
Źródło:
Journal of Plant Protection Research; 2020, 60, 3; 244-252
1427-4345
Pojawia się w:
Journal of Plant Protection Research
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Modified neutral comet assay for human lymphocytes
Autorzy:
Wojewódzka, M.
Grądzka, I.
Buraczewska, I.
Powiązania:
https://bibliotekanauki.pl/articles/147130.pdf
Data publikacji:
2002
Wydawca:
Instytut Chemii i Techniki Jądrowej
Tematy:
DNA double strand breaks
human lymphocyte
neutral comet assay
Opis:
Comet assay under neutral conditions allows the detection of DNA double-strand breaks, considered to be the biologically relevant radiation-induced lesion. In this report we describe modifications of the neutral comet method, which simplify and facilitate its use for estimation of DNA double strand breaks in human lymphocytes irradiated with doses of 60Co gamma-rays (from 10 to 100 Gy). The analysis carried out according to this protocol takes less time than those published so far. Also, the use of lysis at 50°C is avoided; this is important in view of the presence of heat-labile sites in the DNA of irradiated cells, recently reported by Rydberg [12]. The comets have well defined, sharp limits, are suitable for computer image analysis and chromatin of the control cells remains condensed.
Źródło:
Nukleonika; 2002, 47, 1; 1-5
0029-5922
1508-5791
Pojawia się w:
Nukleonika
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
In vitro evaluation of zearalenone toxicity by comet assay
Autorzy:
Harcarova, M.
Conkova, E.
Kolenicova, S.
Holeckova, B.
Proskovcova, M.
Powiązania:
https://bibliotekanauki.pl/articles/16539336.pdf
Data publikacji:
2022
Wydawca:
Polska Akademia Nauk. Czasopisma i Monografie PAN
Tematy:
mycotoxins
toxicity
comet assay
Opis:
The aim of this study was to reveal the potentially genotoxic effect of zearalenone on bovine lymphocytes by comet assay in vitro. The bovine lymphocytes were exposed to various zearalenone concetrations (50; 10; 2; 0.4 and 0.08 ppm). The viability and DNA damage of lymphocytes was monitored after 2 h, 24 h, 48 h and 72 h. After 2 hours of zearalenone exposure, statistically significant DNA damage occurred at all tested concentrations of 0.08 ppm (12.2±1.25; p<0.05), 0.4 ppm (12.7±0.88; p<0.01), 2 ppm (12.0±0.51; p<0.01), 10 ppm (11.2±0.47; p<0.01) and at 50 ppm (14.2±0 61; p<0.001). Significantly greater DNA damage was also found after 24 h, 48 h and 72 h. The obtained results showed that zearalenone may induce DNA damage of the bovine lymphocytes.
Źródło:
Polish Journal of Veterinary Sciences; 2022, 25, 3; 475-477
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Detection of alkylation damage in human lymphocyte DNA with the comet assay.
Autorzy:
Collins, Andrew
Dušinská, Mária
Horská, Alexandra
Powiązania:
https://bibliotekanauki.pl/articles/1044089.pdf
Data publikacji:
2001
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
AlkA
DNA damage
comet assay
Opis:
The enzyme 3-methyladenine DNA glycosylase II (AlkA) is a bacterial repair enzyme that acts preferentially at 3-methyladenine residues in DNA, releasing the damaged base. The resulting baseless sugars are alkali-labile, and under the conditions of the alkaline comet assay (single cell gel electrophoresis) they appear as DNA strand breaks. AlkA is not lesion-specific, but has a low activity even with undamaged bases. We have tested the enzyme at different concentrations to find conditions that maximise detection of alkylated bases with minimal attack on normal, undamaged DNA. AlkA detects damage in the DNA of cells treated with low concentrations of methyl methanesulphonate. We also find low background levels of alkylated bases in normal human lymphocytes.Single cell gel electrophoresis (the comet assay) is widely used for the detection of strand breaks in nuclear DNA. It is particularly appropriate for studying the low background levels of damage present in normal human cells, such as peripheral lymphocytes. The cells are embedded in agarose on a microscope slide and lysed with Triton X-100 and 2.5 M NaCl, which remove cytoplasm and most nuclear proteins, but leave the DNA, in supercoiled form, as nucleoids. After incubation in alkali, the DNA is electrophoresed at high pH; DNA is drawn out to form a 'tail' (hence the name 'comet assay') - but only if breaks are present to relax the supercoiling of the nucleoid DNA. In order to increase its sensitivity and selectivity, we have incorporated into the assay an extra step in which the nucleoid DNA is digested with a lesion-specific endonuclease; the additional breaks revealed with this procedure indicate the presence of the particular lesion. So far, endonuclease III (NTH, specific for oxidised pyrimidines) (Collins et al., 1993), formamidopyrimidine DNA glycosylase (FPG, acting on ring-opened purines and the major purine oxidation produce, 8-oxoguanine) (Dušinská & Collins, 1996) and T4 endonuclease V (recognising UV-induced cyclobutane pyrimidine dimers) (Collins et al., 1997b) have been successfully employed. Amongst other things, we have estimated background levels of DNA oxidation (Collins et al., 1997a), and have found this damage to be elevated in human diseases such as diabetes and ankylosing spondylitis (Dušinská et al., 1999).We now report the use of AlkA, a bacterial repair enzyme whose main substrate is 3-methyladenine in DNA, though it also recognises - with lower efficiency - other modified bases (Lindahl, 1993). A recent report (Berdal et al., 1998) suggests that repair enzymes supposedly specific for alkylated bases may in fact create breaks non-selectively (though much less efficiently) at normal bases. Given the size of the genome, even a low efficiency of non-specific breakage could significantly interfere in estimations of background levels of alkylation damage. We reasoned that, by employing a range of concentrations of the enzyme, and carrying out incubations for different lengths of time, we might find a concentration at which only the alkylated bases would be detected, so that the number of breaks would increase to a certain level and then plateau. After optimising reaction conditions, we tested the assay on lymphocytes from different individuals, and also, as a positive control, examined alkylation damage induced by methyl methanesulphonate.
Źródło:
Acta Biochimica Polonica; 2001, 48, 3; 611-614
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Test mikrojądrowy w retrospektywnej dozymetrii biologicznej
Micronucleus assay in rapid retrospective biological dosimetry
Autorzy:
Rawojć, K.
Miszczyk, J.
Powiązania:
https://bibliotekanauki.pl/articles/134746.pdf
Data publikacji:
2016
Wydawca:
ADVSEO
Tematy:
micronuclei
micronucleus assay
retrospective biological dosimetry
ionizing radiation
cell death
Opis:
Rapid retrospective biological dosimetry allows absorbed dose evaluation post exposure to ionizing radiation. One of the main tools of biodosimetry is based on the analysis of the effects resulting from the impact of ionizing radiation on the cell. Various cytogenetic tests give possibility of the accurate dose estimation. To investigate cell response to radiation one performs the analysis of biomarkers approved by International Atomic Energy Agency e.g. the analysis of dicentric chromosomes or micronuclei frequency. Micronucleus test is relatively a faster and therefore more effective method to study changes in the genetic material, induced by various genotoxic agents. This study confirms that micronulei frequency and nuclear division index analysis allows for appropriate absorbed dose estimation when it comes to ionizing radiation. In order to further optimize and facilitate the micronucleus assay and other cytogenetic tests in rapid retrospective biological dosimetry, the research are still ongoing.
Źródło:
Technical Issues; 2016, 4; 78-82
2392-3954
Pojawia się w:
Technical Issues
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The use of Allium cepa L. assay as bioindicator for the investigation of genotoxic effects of industrial waste water
Autorzy:
Rasgele, Pinar Goc
Powiązania:
https://bibliotekanauki.pl/articles/2073789.pdf
Data publikacji:
2021
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
Allium cepa assay
mitotic index
waste water
Opis:
In this study, genotoxic potential of industrial waste water (IWW) samples was investigated using Allium cepa assay. The root tips were treated with different IWW samples (A, B and C) for 48 hours. The effects of IWW on cytological effects were determined. It was found that all IWW samples significantly increased the percentage of total abnormality. Mitotic chromosomal abnormalities such as irregular metaphase, stickiness, c-mitosis, micronucleus, vagrant chromosomes and bridges were determined. Furthermore, a significant reduction for the mitotic index that is indicative of cellular toxicity was observed in root tips cells, which were treated with IWW samples. A. cepa assay can be used as useful tool for the detection of genotoxic and cytotoxic potential of IWWs.
Źródło:
Archives of Environmental Protection; 2021, 47, 4; 3--8
2083-4772
2083-4810
Pojawia się w:
Archives of Environmental Protection
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Aggressiveness of Fusarium langsethiae isolates towards wheat, barley and oats in an in vitro leaf assay.
Autorzy:
Opoku, Nelson
Back, Matthew
Edwards, Simon G.
Powiązania:
https://bibliotekanauki.pl/articles/2199610.pdf
Data publikacji:
2011-12-20
Wydawca:
Instytut Hodowli i Aklimatyzacji Roślin
Tematy:
aggressiveness
Fusarium langsethiae
HT-2
in vitro
leaf assay
T-2
trichothecenes
Opis:
Fusarium langsethiae has been identified as the primary producer of HT-2 and T-2 in European cereals. HT-2 and T-2 are considered as two of the most potent trichothecenes mycotoxins and a public health concern in Europe. There is currently no legislation on HT-2 and T-2, however, there is a discussion limit of 500 µg kg -1  in unprocessed oats, 200 µg kg-1 for oat products and 50 µg kg-1 for infant food. There are limited data regarding F. langsethiae’s pathogenicity and mycotoxin production, but it is evident that its behaviour deviates from traditional trichothecene-producing Fusarium species. This experiment was aimed at assessing the aggressiveness (measured by lesion length) of 20 different F. langsethiae isolates on wheat, barley and oats using an in vitro detached leaf assay. There was a significant (P<0.001) difference between lesion lengths formed by different F. langsethiae isolates used. Isolate Fl/2004/17(a) caused the shortest lesion on all cereals and this was significantly (P<0.001) different from that caused by isolate Fl/0/08/009/1 which caused the longest lesion on all cereals used. A highly significant difference (P<0.001) was also observed between lesions on the different cereals (wheat, barley and oats). Lesions on oats were the longest, followed by barley with wheat showing the shortest lesions.
Źródło:
Plant Breeding and Seed Science; 2011, 64; 55-64
1429-3862
2083-599X
Pojawia się w:
Plant Breeding and Seed Science
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Utworzenie Urzędu Probierczego w Krakowie i jego działalność w latach 1843–1853
Establishment of the Assay Office in Kraków and its activities in the years 1843–53
Autorzy:
Mataniak, Mateusz
Powiązania:
https://bibliotekanauki.pl/articles/965379.pdf
Data publikacji:
2016
Wydawca:
Krakowska Akademia im. Andrzeja Frycza Modrzewskiego
Tematy:
Free City of Kraków
Assay Office
Jewellers and Goldsmiths Guild
jewellery
assay of gold
Wolne Miasto Kraków
Urząd Probierczy
cech jubilerów i złotników
wyroby jubilerskie
próba karatowa
Opis:
The article presents the circumstances of setting up the Assay Office in the Free City of Kraków and also its activity in 1843–53. The source base consisted of regulations of the Governing Senate on the organisation and competence of the Assay Office, and documentation related to its activity, being a part of the complex of the Archive of the Free City of Kraków. The fundamental task of the Assay Office was to supervise Kraków jewellers and goldsmiths. The supervision especially focused on gold and silver objects brought for stamping. The office charged fees defined by the government for its services. The article also mentions prosecution of crimes against the assay stamp, which was a prerogative of the administrative authorities: the Senate, and later the Administrative Council and the City Council
W artykule przedstawiono okoliczności powstania Urzędu Probierczego w Wolnym Mieście Krakowie, a także przebieg jego działalności w latach 1843–1853. Podstawę źródłową stanowiły rozporządzenia Senatu Rządzącego dotyczące organizacji i kompetencji Urzędu Probierczego, a także związana z jego działalnością dokumentacja, stanowiąca część zespołu Archiwum Wolnego Miasta Krakowa. Podstawowym zadaniem Urzędu Probierczego było nadzorowanie krakowskich jubilerów i złotników, przy czym kontrola obejmowała zwłaszcza przynoszone przez nich do ostemplowania wyroby ze złota i srebra. Za swoje czynności urząd pobierał opłaty według taksy rządowej. W artykule wspomniano też o ściganiu przestępstw probierczych, co należało do władz administracyjnych: Senatu, a następnie Rady Administracyjnej i Rady Miejskiej.
Źródło:
Studia z Dziejów Państwa i Prawa Polskiego; 2016, 19; 179-198
1733-0335
Pojawia się w:
Studia z Dziejów Państwa i Prawa Polskiego
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Fluorometric assay of oleate-activated phospholipase D isoenzyme in membranes of rat nervous tissue and human platelets
Autorzy:
Krzystanek, Marek
Trzeciak, Henryk
Krzystanek, Ewa
Małecki, Andrzej
Powiązania:
https://bibliotekanauki.pl/articles/1040386.pdf
Data publikacji:
2010
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
human platelets
membranes
rat brain
fluorometric assay
phospholipase D
astrocytes
Opis:
Phospholipase D plays a key role in the biosynthesis of phosphatidic acid, a second messenger involved in essential cellular processes. Oleate-activated phospholipase D was the first mammalian phospholipase D isoform to be discovered but is the least known. The study was aimed to test a fluorometric method of assessment of oleate-activated phospholipase D activity in different biological materials. The brain cortex of male Wistar rats, cultured rat brain astrocytes, and human platelets were processed to yield plasmatic membranes for experiments. To assess phospholipase D activity the modified fluorometric method was used. Previously, the method was used only to determine H2O2. In this enzyme-coupled assay phospholipase D activity is monitored indirectly using 10-acetyl-3,7-dihydroxyphenoxazine. First, phospholipase D cleaves exogenous phosphatidylcholine to yield choline and phosphatidic acid. Second, choline is oxidized by choline oxidase to betaine and H2O2. Finally, in the presence of horseradish peroxidase, H2O2 reacts with 10-acetyl-3,7-dihydroxyphenoxazine to generate the highly fluorescent product, resorufin. The concentration of resorufin was measured using excitation and emission at 560 nm and 590 nm, respectively. The proposed optimal parameters of the tested assay are 25 µg of rat brain cortex protein, 50 µg of rat brain astrocyte protein, and 50 µg of human platelet protein in a reaction volume of 200 µL, and 2 min enzymatic reaction at 37°C. The fluorometric method may be applied to assay phospholipase D in different biological materials.
Źródło:
Acta Biochimica Polonica; 2010, 57, 3; 369-372
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Is concentration and motility of male gametes related to DNA damage measured by comet assay?
Autorzy:
Dobrzynska, M M
Tyrkiel, E.J.
Derezinska, E.
Ludwicki, J.K.
Powiązania:
https://bibliotekanauki.pl/articles/50202.pdf
Data publikacji:
2010
Wydawca:
Instytut Medycyny Wsi
Tematy:
spermatozoon
male
germ cell
DNA damage
motility
comet assay
sperm quality
Źródło:
Annals of Agricultural and Environmental Medicine; 2010, 17, 1; 73-77
1232-1966
Pojawia się w:
Annals of Agricultural and Environmental Medicine
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Application of the micronucleus assay performed by different scorers in case of large-scale radiation accidents
Autorzy:
Rawojć, K.
Tarnawska, D. M.
Miszczyk, J. U.
Swakoń, J.
Stolarczyk, L.
Rydygier, M.
Powiązania:
https://bibliotekanauki.pl/articles/146534.pdf
Data publikacji:
2015
Wydawca:
Instytut Chemii i Techniki Jądrowej
Tematy:
biological dosimetry
cytokinesis-blocked micronucleus assay
frequency of micronuclei
Opis:
Mass casualty scenarios of radiation exposure require high throughput biological dosimetry techniques for population triage, in order to rapidly identify individuals, who require clinical treatment. Accurate dose estimates can be made by biological dosimetry, to predict the acute radiation syndrome (ARS) within days after a radiation accident or a malicious act involving radiation. Timely information on dose is important for the medical management of acutely irradiated persons [1]. The aim of the study was to evaluate the usefulness of the micronuclei (MNi) scoring procedure in an experimental mode, where 500 binucleated cells were analyzed in different exposure dose ranges. Whole-body exposure was simulated in an in vitro experiment by irradiating whole blood collected from one healthy donor with 60 MeV protons and 250 keV X-rays, in the dose range of 0.3–4.0 Gy. For achieving meaningful results, sample scoring was performed by three independent persons, who followed guidelines described in detail by Fenech et al. [2, 3]. Compared results revealed no signifi cant differences between scorers, which has important meaning in reducing the analysis time. Moreover, presented data based on 500 cells distribution, show that there are significant differences between MNi yields after 1.0 Gy exposure of blood for both protons and X-rays, implicating this experimental mode as appropriate for the distinction between high and low dose-exposed individuals, which allows early classification of exposed victims into clinically relevant subgroups.
Źródło:
Nukleonika; 2015, 60, No. 3, part 2; 643-649
0029-5922
1508-5791
Pojawia się w:
Nukleonika
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
APPROACH OF THE STATE PHARMACOPEIA OF UKRAINE TO ANALYTICAL PROCEDURES VALIDATION ON THE EXAMPLE OF CHLORIDE IONS ASSAY IN PERITONEAL DIALYSIS SOLUTIONS
Autorzy:
Hudz, Nataliia
Leontiev, Dmytro
Wieczorek, Piotr P.
Powiązania:
https://bibliotekanauki.pl/articles/895322.pdf
Data publikacji:
2019-08-30
Wydawca:
Polskie Towarzystwo Farmaceutyczne
Tematy:
uncertainty
validation
solutions for peritoneal dialysis
chlorides assay
argentometric method
Opis:
The objective of this study was to develop and validate an alternative analytical procedure for the total chloride assay in solutions for peritoneal dialysis (PD). The proposed analytical procedure was validated according to the requirements of the International Conference on Harmonization guideline: Topic ICH Q2(R1) and the approach of the State Pharmacopeia of Ukraine (SPU). The analytical procedure was specific. The linearity of the procedure was evaluated in the concentration range of 76 to 114 mmol/L of chloride ions (80-120 % of the stated content 95 mmol/L) with the regression equation y=1.0029•X-0.2263 and a correlation coefficient of 0.9989. The y-intercept of the regression line did not exceed the maximum permissible value of 2.6. The residual standard deviation (s0=0.65) of the calibration curve met the requirement for max s0 (0.84). The mean recovery was found as 100.07%±0.62 %. The precision study also showed a low value of one-sided 95% confidence limit (∆Z=1.15%) that did not exceed the critical value of 1.6%. The accuracy study also showed that the systematic error had not differed statistically from zero. The developed analytical procedure was also found to be robust and reproducible as contents differences were less 1.6%. The reproducibility studies were conducted with different samples of the same laboratory-made PD solutions in different days and laboratories. The performed studies indicated that the developed analytical procedure is simple, fast and cost-efficient, specific, linear, precise, accurate, and robust. The presented approach could be also applied to the validation of other assay analytical procedures.
Źródło:
Acta Poloniae Pharmaceutica - Drug Research; 2019, 76, 4; 635-643
0001-6837
2353-5288
Pojawia się w:
Acta Poloniae Pharmaceutica - Drug Research
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Assay of the thermally stable, insensitive, high explosive 1,3,5-triamino-2,4,6-trinitrobenzene (TATB)
Autorzy:
Nandi, A. K.
Thirupathi, N.
Mandal, A. K.
Pandey, P. K.
Powiązania:
https://bibliotekanauki.pl/articles/358608.pdf
Data publikacji:
2014
Wydawca:
Sieć Badawcza Łukasiewicz - Instytut Przemysłu Organicznego
Tematy:
assay TATB
acid-base titration
base hydrolysis
trinitrophloroglucinol
ammonium chloride
Opis:
An aqueous titration method is described to assay the insensitive, high explosive 1,3,5-triamino-2,4,6-trinitrobenzene (TATB). TATB is initially quantitatively converted to the weak acid 1,3,5-trihydroxy-2,4,6-trinitrobenzene (THTNB) by base catalysed hydrolysis. Subsequently THTNB is assayed by acidbase titration. TATB samples obtained from regular batch operations are assayed by this method, and the results are compared with those obtained by the total amino functional group estimation method using a modified Kjeldhal apparatus. The method is simple and has good accuracy and precision.
Źródło:
Central European Journal of Energetic Materials; 2014, 11, 2; 295-305
1733-7178
Pojawia się w:
Central European Journal of Energetic Materials
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Resistance of potato tubers to Phytophthora infestans evaluated in laboratory tests and field trials
Autorzy:
Rogozina, Elena V.
Patrikeyeva, Maria V.
Powiązania:
https://bibliotekanauki.pl/articles/2198858.pdf
Data publikacji:
2004-12-20
Wydawca:
Instytut Hodowli i Aklimatyzacji Roślin
Tematy:
advanced potato clone
field trial
Phytophthora infestans
tetraploid interspecific hybrid
tuber resistance
tuber slices assay
whole tubers assay
Opis:
Thirty potato genotypes of different maturity were evaluated for tuber blight resistance both under laboratory and field conditions. From 2000 to 2003 replicated experiments were conducted with three potato cultivars registered in Russia and 27 tetraploid interspecific hybrids that were generated at VIR. Each genotype was assessed twice using laboratory tests, and its resistance was evaluated every year in a field trial. The results showed that four hybrid clones: 88-2, 95-23-3, 97-152-6 and 97-162-5 exhibited a superior performance of tuber resistance to Phytophthora infestans both in laboratory tests and field trials. Two cultivars and 10 hybrid clones were found susceptible to infection in all tests. Significant differences between the remaining 14 genotypes in tuber slice resistance, whole tuber resistance and the expression of resistance to tuber blight in the field were observed.
Źródło:
Plant Breeding and Seed Science; 2004, 50; 147-154
1429-3862
2083-599X
Pojawia się w:
Plant Breeding and Seed Science
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Development of an indirect enzyme-linked immunosorbent assay for the detection of novel chicken orthoreovirus
Autorzy:
Wei, Z.
Yang, J.
Hu, J.
Tang, Y.
Diao, Y.
Powiązania:
https://bibliotekanauki.pl/articles/16539093.pdf
Data publikacji:
2022
Wydawca:
Polska Akademia Nauk. Czasopisma i Monografie PAN
Tematy:
novel avian orthoreovirus
σC gene
recombinant plasmid
indirect enzyme linked immunosorbent assay
epidemiological investigation
Opis:
A novel avian orthoreovirus (N-ARV) variant characterized with obvious arthritis and synovial inflammation, was isolated from Shandong, China in May 2016. It caused chicken poor growth and enormous economic losses to the poultry industry of China. However, there are few effective methods for detecting the antibody levels of N-ARV. In this study, a viral structural protein σC was expressed using the prokaryotic expression vector pET32a (+). The target protein was obtained by inducing for 6 hours at an IPTG concentration of 0.6mM. The optimal dilution of the coating antigen and serum antibody were determined to be 1000 fold and 10 fold respectively. A specificity test showed that there was no positive reactivity between N-ARV and other pathogens, and when the positive serum was diluted 100 times detection results were still checkable. The repeatability of this method was determined by the inter assay and intra assay tests with variability ranging from 4.85% to 7.93%. In conclusion, this indirect enzyme linked immunosorbent assay (ELISA) will be useful for large-scale serological surveys and monitoring antibody levels in N-ARV infection.
Źródło:
Polish Journal of Veterinary Sciences; 2022, 25, 1; 109-118
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Teologia społeczna ruchu migracyjnego. Próba zarysu
The Social Theology of Migration Movements. Assay of Appreciation
Autorzy:
Woźnicki, Andrzej
Powiązania:
https://bibliotekanauki.pl/articles/1991532.pdf
Data publikacji:
1977
Wydawca:
Katolicki Uniwersytet Lubelski Jana Pawła II. Towarzystwo Naukowe KUL
Opis:
The phenomenon of migration belongs to. principal problems of the contemporary international life. To its importance testifies the fact that only in the 1946— 1954 years in migration was involve about 8 millions persons. The migration movement grows steadily and is followed by many social problems, both within the local and migration populations. In such a situation the Church must take an appropriate stand towards it and — depending on actual exigencies — elaborate the adequate methods of action in this domain. To some extent this need is met by the decisions of the Apostolic See: the Apostolic Constitution „Exsul familia” (1952) the instruction „De pastoralis migratorum cura” (1969), and by decisions of Vatican' Council II. The fact of differentiating populations by migration makes it susceptible to studies from different points of view and in the light of varoius sciences. This work in an attempt to a migration movement description basing on the Catholic social theology principles, elaborated by Vatican Council II: and applied in the .Apostolic See’s instruction ,,De pastoralis migratorum cura”. The theological basis for reflections on religious importance of the migration movement constitutes the Council Fathers teaching On the pilgriming Church, on collegial Church administration ecumenism and religious tolerance. According to the doctrine all Christians are pilgriming to the heavenly Father and on the Earth they are only guests. The ’biblical picture of the earthly pilgrimage is realized in the Christ’s Church, i.e. pilgriming Church. Thus, to be an immigrant is one form of human pilgriming to the heavenly homeland. Such an understanding of Christian life gives to every immigrant’s life a symbol of the Christ wandering about the Earth. In effect the migration assumes an eschatological character. As the whole Church is pilgriming, it is why she from her very beginnings especially cared for the migrants; the migratory movement helped the Church to grow. The migration issue is influenced by the collegial-Church-government principle. The universalistic understanding of Church government collegiality gives a sense of responsibility to all God’s people for the fate of the brothers living in the migration situation. Therefore the apostolic activity among emigrants should belong not only to compencies of local ordinaries or episcopal conferences of migrants’ dwelling countries, because the local authorities often dispel a lack of understanding of national-religious needs of the religious-ethnic minorities. From it results a necessity of collaboration between the hierarchy of the origin-place and the hierarchy of the present migrants’ .abode. The postulate is important although difficult to realize. The third basis of theological reflection on the migratory movements is a new understanding of ecumenism within the Church. It must be inspired by a atmosphere of religious tolerance. The properly conceived ecumenism and tolerance demand the respecting of distinctness of particular population groups; it is then an •organic unity within multiplicity can be created. Populational migrations in a great measure contribute to overcoming the difficulties in our world and thereby bring closer all the nations. The migration movement is one principal factor of mutual understanding within a society. An appropriate planning of the movements can allow to relieve the tension between nations and will much contribute to maintaining world peace. The migratory movement permits to solve several moral and religious problems and can create occasions the new culture-creating values to originate; it is of significant importance also for the missionary work of the Church. The alignment to a nationality must be determined by personal choice and decision of each migrant individual. Cultivation of a national-ethnic distinctness is today possible only with a conscious cooperation of both sides. This demands of mutual knowledge and understanding. Only in a full confidence and mutual friendliness atmosphere the exchange of values is possible. The Christian, living in the migrant situation, being a sing and symbol of Christ- Pilgrim on the Earth becomes a living witness of the Christ’s truth. In various ways the migrant-Christian can give witness to this truth: 1. by apostolate of abiding in unbelieving environment, 2. by apostolate of pronouncing the God’s word, 3. by the apostolate of one’s own deeds, in a word, by living with faith. In the migration situation the faith inherited is generally lost, and demand is of a faith personally experienced and deepened. It is a faith from one’s own choice. In propagating of one’s own group culture one should add to its attractiveness by shoving the culture in connections with all-human culture. Such an approach to cultural life of one’s own ethnic group will simultaneously allow to bring nearer the migrant and host-country populations and will permit the migrants to became full citizens of the new homeland. Thus the Christian migrant will be a catalyzer precipitating the process of unifying the whole mankind in a one Human Family. This work is supplemented by an annex containing considerations about a necessity of conducting the sociological investigations within migratory population, basing on example of the Polonia community in the United States.
Źródło:
Studia Polonijne; 1977, 2; 5-81
0137-5210
Pojawia się w:
Studia Polonijne
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
DNA damage and repair in lymphocytes of normal individuals and cancer patients: studies by the comet assay and micronucleus tests.
Autorzy:
Palyvoda, Olena
Polańska, Joanna
Wygoda, Andrzej
Rzeszowska-Wolny, Joanna
Powiązania:
https://bibliotekanauki.pl/articles/1043662.pdf
Data publikacji:
2003
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
ionizing radiation
DNA damage
human lymphocytes
comet assay
head and neck tumors
DNA repair
Opis:
A population study is reported in which the DNA damage induced by γ-radiation (2 Gy) and the kinetics of the subsequent repair were estimated by the comet and micronucleus assays in isolated lymphocytes of 82 healthy donors and patients with head and neck cancer before radiotherapy. The parameters of background and radiation-induced DNA damage, rate of repair, and residual non-repaired damage were measured by comet assay, and the repair kinetics for every donor were computer-fitted to an exponential curve. The level of background DNA damage before irradiation measured by comet assay as well as the level of micronuclei were significantly higher in the head and neck cancer patient group than in the healthy donors, while the parameters of repair were widely scattered in both groups. Cancer patient group contained significantly more individuals, whose irradiated lymphocytes showed high DNA damage, low repair rate and high non-repaired DNA damage level. Lymphocytes of donors belonging to this subgroup showed significantly lower inhibition of cell cycle after irradiation.
Źródło:
Acta Biochimica Polonica; 2003, 50, 1; 181-190
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Expression of bovine leukemia virus protein p24 in Escherichia coli and its use in the immunoblotting assay.
Autorzy:
Bicka, Leokadia
Kuźmak, Jacek
Kozaczyńska, Bożena
Płucienniczak, Andrzej
Skorupska, Anna
Powiązania:
https://bibliotekanauki.pl/articles/1044191.pdf
Data publikacji:
2001
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
bovine leukemia virus
fusion protein p24
immunoblotting assay
gag gene cloning
Opis:
The gag gene encoded protein, p24 of bovine leukemia virus (BLV), was cloned and expressed as thioredoxin-6xHis-p24 protein in Escherichia coli. The bacterial cells carrying plasmid pT7THis-p24 expressed the protein of 38 kDa that was detected by immunoblotting analysis using anti-p24 monoclonal antibodies and sera from BLV infected cattle and sheep. The purified p24 fusion protein was shown to be sensitive and specific for detection of BLV antibodies in the infected cattle.
Źródło:
Acta Biochimica Polonica; 2001, 48, 1; 227-232
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
DNA double-strand break rejoining in radioadapted human lymphocytes: evaluation by neutral comet assay and pulse-field gel electrophoresis
Autorzy:
Wojewódzka, M.
Buraczewska, I.
Szumiel, I.
Grądzka, I.
Powiązania:
https://bibliotekanauki.pl/articles/146153.pdf
Data publikacji:
2006
Wydawca:
Instytut Chemii i Techniki Jądrowej
Tematy:
human lymphocytes
radioadaptation
DNA double-strand break rejoining
neutral comet assay
pulsefield gel electrophoresis
Opis:
Adaptive response (AR), an enhanced resistance to a high dose of ionising radiation acquired after pretreatment with a very low dose, was estimated in normal human lymphocytes. The question posed was whether the extent of radioadaptation, assessed by micronucleus test, would be related to the rate of DNA double-strand break (DSB) rejoining. Phytohemagglutinin-stimulated G1-lymphocytes from 5 healthy male volunteers were pre-treated (or not) with an adaptive (5 cGy) dose of X-rays, followed by a higher (5 or 10 Gy) challenge dose after 20-22 h. DSB rejoining after the challenge dose was monitored with the use of two methods: neutral comet assay, modified to reduce the contribution of single-strand breaks (SSBs) and thermolabile sites, and pulse-field gel electrophoresis (PFGE), specific for DSBs. At the level of micronuclei, an AR was observed in lymphocytes of 3 of 5 donors. Up to 60 min, comet assay showed no statistically significant differences in DNA break rejoining between adapted and non-adapted lymphocytes, independently of AR appearance. PFGE gave similar results, although in three donors it revealed secondary increases in DSBs levels at 30 min and/or 60 min post-irradiation in the adapted vs. the non-adapted samples. Failure to demonstrate changes in DSBs rejoining rate in the adapted lymphocytes could be due to diversity of AR intensity/timing at the level of DNA repair in not fully homogenous cell populations. Also, “rare” DNA cuts characteristic of early apoptosis/necrosis could overlap the process of DNA break rejoining.
Źródło:
Nukleonika; 2006, 51, 4; 185-191
0029-5922
1508-5791
Pojawia się w:
Nukleonika
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Prosta metoda oznaczania immunoglobulin
Prostojj metod opredelenija immunoglobulinov
A simple method for immunoglobulins assay
Autorzy:
Gutkowska, J.
Chmielewska, D.
Powiązania:
https://bibliotekanauki.pl/articles/2189605.pdf
Data publikacji:
1972
Wydawca:
Polskie Towarzystwo Diagnostyki Laboratoryjnej
Źródło:
Diagnostyka Laboratoryjna; 1972, 08, 1; 45-51
0867-4043
Pojawia się w:
Diagnostyka Laboratoryjna
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Evaluation of DNA damage in white blood cells of healthy human volunteers using the alkaline comet assay and the chromosome aberration test
Autorzy:
Kopjar, Nevenka
Želježić, Davor
Garaj-Vrhovac, Verica
Powiązania:
https://bibliotekanauki.pl/articles/1041246.pdf
Data publikacji:
2006
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
peripheral blood
chromosome aberration test
white blood cells
DNA damage
alkaline comet assay
lymphocytes
Opis:
The present study was undertaken to contribute to the characterization of the degree of variability in baseline damage in white blood cells from control population, and to investigate how this variability is associated with external and internal factors. Altogether 170 healthy volunteers, randomly selected from the general population of the Republic of Croatia, participated in the study. Two sensitive tests: the alkaline comet assay and the chromosome aberration test were applied to study the background levels of DNA damage in their white blood cells. The results point to inter-individual differences, indicating different genome sensitivity. As revealed by both assays, the background levels of DNA damage were mostly influenced by smoking habit as well as medical exposure (especially to diagnostic X-rays). Sex and age of subjects did not significantly influence the values of DNA damage recorded in the white blood cells. Although higher levels of DNA damage were recorded in blood samples collected during winter and autumn, they were mostly influenced by medicinal exposure and smoking habit. Statistical evaluation of the data confirmed that a positive correlation exists between DNA migration and the number of long-tailed nuclei found with the comet assay and the total number of chromosome aberrations. The data obtained can serve as control values in forthcoming biomonitoring studies.
Źródło:
Acta Biochimica Polonica; 2006, 53, 2; 321-336
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Micronucleus assay in epithelial cells from the oral cavity and urinary tract in female smokers and non-smokers
Autorzy:
Błaszczyk, E.
Mielzynska-Svach, D.
Powiązania:
https://bibliotekanauki.pl/articles/363246.pdf
Data publikacji:
2014
Wydawca:
Uniwersytet Warmińsko-Mazurski w Olsztynie
Tematy:
epithelial cells
micronucleus assay
oral cavity
tobacco smoking
urinary tract
komórki nabłonkowe
test mikrojądrowy
jama ustna
palenie tytoniu
drogi moczowe
Opis:
The aim of the work was to investigate the possible use of epithelial cells from the oral cavity and urinary tract in identifying smoking-related effects in women. Epithelial cells from the oral cavity and urinary tract were collected from 9 smoking and 9 non-smoking women and subjected to micronucleus assay. The DNA damage (cells with micronuclei and nuclear buds), cytokinetic defects (binucleated cells) and cell death (condensed chromatin, karyorrhexis, pyknosis and karyolysis) were observed after DNA specific staining. In pooled analysis of the frequency of binucleated cells and condensed chromatin cells in 18 studied women, statistically significant differences were noted only in epithelial cells from the oral cavity in comparison to those of the urinary tract. Non pooled results demonstrated no differences in damage frequency in cells collected from the oral cavity and isolated from the urine. The lack of differences in the observed frequencies of micronuclei in buccal and urothelial cells could be an effect of the small size of the sampled group, smoking pattern of the women and the number of cigarettes smoked per day.
Źródło:
Environmental Biotechnology; 2014, 10, 2; 60-65
1734-4964
Pojawia się w:
Environmental Biotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Lasery w urzędach probierczych
Lasers in assay offices
Autorzy:
Wytrykus, Beata
Powiązania:
https://bibliotekanauki.pl/articles/952646.pdf
Data publikacji:
2016
Wydawca:
Główny Urząd Miar
Tematy:
lasery
znakowanie laserowe
probiernictwo
lasers
laser marking
hallmarking
Opis:
W artykule opisano rodzaje laserów oraz zastosowanie laserowych urządzeń do oznaczania wyrobów z metali szlachetnych w Okręgowym Urzędzie Probierczym w Warszawie.
The article describes the types of lasers and the application of laser for marking of precious metals in Regional Assay Office in Warsaw.
Źródło:
Metrologia i Probiernictwo : biuletyn Głównego Urzędu Miar; 2016, 1 (12); 53-56
2300-8806
Pojawia się w:
Metrologia i Probiernictwo : biuletyn Głównego Urzędu Miar
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Genotoxicity of the organophosphorus compound methylparaoxon evaluated by the comet assay
Autorzy:
Trzeciak, Andrzej
Błasiak, Janusz
Wojewódzka, Maria
Kowalik, Joanna
Powiązania:
https://bibliotekanauki.pl/articles/895975.pdf
Data publikacji:
2001
Wydawca:
Uniwersytet Łódzki. Wydawnictwo Uniwersytetu Łódzkiego
Opis:
Pesticides are widely used in agriculture, food production and household. Due to the broad spectrum of their activity, their biological action is extensively investigated. We assessed the genotoxidty of methylparaoxon - the main metabolite of commonly used organophosphorus insecticide, methylparathion. Freshly isolated human peripheral blood lymphocytes were incubated for 1 h with methylparaoxon at 37° C, and then, were incubated in methylparaoxon-free medium to examine DNA repair. Alkaline single cell gel electrophoresis (the comet assay) was used to assess DNA damage and repair. Methylparaoxon induced moderate DNA damages that were almost completely repaired after 60 min. Obtained results suggest that methylparaoxon could induce single-strand breaks in DNA both directly and by methylathion of DNA bases.
Źródło:
Acta Universitatis Lodziensis. Folia Biochimica et Biophysica; 2001, 15
0208-614X
Pojawia się w:
Acta Universitatis Lodziensis. Folia Biochimica et Biophysica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Moneta w urzędzie probierczym
Coin at the assay office
Autorzy:
Maciejowski, Robert
Powiązania:
https://bibliotekanauki.pl/articles/952581.pdf
Data publikacji:
2017
Wydawca:
Główny Urząd Miar
Tematy:
złote monety
złotnictwo
probiernictwo
gold coins
goldsmithing
hallmarking
Opis:
Niniejsza publikacja prezentuje metody badania złotych monet, zgłaszanych przez osoby prywatne, firmy złotnicze i instytucje państwowe (policja, prokuratura, urzędy celne) do urzędów probierczych, w celu określenia próby. Publikacja zawiera także wykaz monet, które były najczęściej badane w Wydziale Technicznym Okręgowego Urzędu Probierczego w Krakowie oraz rezultaty przeprowadzonych badań.
This publication presents the methods of testing of gold coins, submitted by private persons, gold-smiths and state institutions (police, prosecutors and customs office) to assay offices, to verify their fineness. The publication includes also the list of coins which were mostly testing at the Technical Department of the Regional Assay Office in Cracow and results of these tests.
Źródło:
Metrologia i Probiernictwo : biuletyn Głównego Urzędu Miar; 2017, 1-2 (16-17); 40-46
2300-8806
Pojawia się w:
Metrologia i Probiernictwo : biuletyn Głównego Urzędu Miar
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Metoda oznaczania kwaśnych mukopolisacharydów osocza
Method of assay of plasma acid mucopolysacharides
Autorzy:
Chmiel, J.
Błażejewska, B.
Suszka, B.
Waliszewski, S.
Powiązania:
https://bibliotekanauki.pl/articles/2189243.pdf
Data publikacji:
1975
Wydawca:
Polskie Towarzystwo Diagnostyki Laboratoryjnej
Źródło:
Diagnostyka Laboratoryjna; 1975, 11, 4; 309-318
0867-4043
Pojawia się w:
Diagnostyka Laboratoryjna
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
A rapid qualitative assay for detection of Clostridium perfringens in canned food products
Autorzy:
Dave, Gayatri
Powiązania:
https://bibliotekanauki.pl/articles/1038632.pdf
Data publikacji:
2017
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
Clostridium perfringens
lecithinase
peranitrophenyl phosphotidyl choline ester
Opis:
Clostridium perfringens (MTCC 1349) is a Gram-positive, anaerobic, endospore forming, and rod-shaped bacterium. This bacterium produces a variety of toxins under strict anaerobic environment. C. perfringens can grow at temperatures ranging between 20°C and 50°C. It is the major causetive agent for gas gangrene, cellulitis, septicemia, necrotic enteritis and food poisoning, which are common toxin induced conditions noted in human and animals. C. perfringens can produce produce four major types of toxins that are used for the classification of strains, classified under type A-E. Across the globe many countries, including the United States, are affected by C. perfringens food poisonings where it is ranked as one of the most common causes of food borne infections. To date, no direct one step assay for the detection of C. perfringens has been developed and only few methods are known for accurate detection of C. perfringens. Long detection and incubation time is the major consideration of these reporter assays. The prensent study proposes a rapid and reliable colorimetric assay for the detection of C. perfringens. In principale, this assay detects the para nitrophenyl (yellow colour end product) liberated due to the hydrolysis of paranitrophenyl phosphetidyl choline (PNPC) through phospholipase C (lecithinase). Constitutive secretion of phospholipase C is a charactristic feature of C. perfringens. This assay detects the presence of the extracellular lecithinse through the PNPC impragnated impregnated probe. The probe is impregnated with peranitrophenyl phosphotidyl choline ester, which is colourless substrate used by lecithinase. The designed assay is specific towards PNPC and detectes very small quantites of lecithinase under conditions used. The reaction is substrate specific, no cross reaction was observed upon incubation with other substrates. In addition, this assay gave negative results with other clostridium strains, no cross reactions were observed with other experimental strains like C. tetani, C. botulinum, C. acetobutyricum, Bacillus subtilis, and Escherichia coli. This assay is extramly rapid and provides reliable and reproducible results within one hour of incubation at 37°C.
Źródło:
Acta Biochimica Polonica; 2017, 64, 2; 207-213
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The value of Fluorescence Polarisation Assay in verification of problematic sera from pigs for brucellosis
Autorzy:
Weiner, M.
Szulowski, K.
Iwaniak, W.
Powiązania:
https://bibliotekanauki.pl/articles/31794.pdf
Data publikacji:
2012
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
The aim of the study was an evaluation of fluorescence polarisation assay (FPA) as a potential tool improving specificity of serological diagnosis of brucellosis in pigs. The evaluation was done by comparing the results of FPA with the results of rose bengal test (RBT), serum agglutination test (SAT), complement fixation test (CFT) and ELISA when false positive sera were tested. One hundred ninety porcine samples, reacting positively in at least one classical serological assay were used. We observed that among 198 sera, 104 were also positive in FPA. The studies confirmed that porcine FPA adds little as far as specificity in comparison to other methods is concerned.
Źródło:
Polish Journal of Veterinary Sciences; 2012, 15, 4
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Isolation of Proteins From Potato Tubers
Autorzy:
Murawska, Zofia
Dębski, Janusz
Szajko, Katarzyna
Lebecka, Renata
Powiązania:
https://bibliotekanauki.pl/articles/2199680.pdf
Data publikacji:
2017-06-20
Wydawca:
Instytut Hodowli i Aklimatyzacji Roślin
Tematy:
bicinchoninic acid assay
mass spectrometry
tuber proteomes
Opis:
Here we optimized an efficient and reproducible method for proteins isolation from potato tubers for quan-titative proteomic analysis, aimed at detection of differentially expressed proteins upon various experimental conditions.
Źródło:
Plant Breeding and Seed Science; 2017, 75; 23-27
1429-3862
2083-599X
Pojawia się w:
Plant Breeding and Seed Science
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Validation of the assay and purity determination of the FOX-7 (1,1-diamino-2,2-dinitroethylene) using HPLC method
Walidacja oznaczania czystości i zawartości FOX 7 (1,1-diamino-2,2-dinitroetylenu) metodą HPLC
Autorzy:
Kamieńska-Duda, A.
Kasprzak, P.
Powiązania:
https://bibliotekanauki.pl/articles/235621.pdf
Data publikacji:
2014
Wydawca:
Wojskowy Instytut Techniczny Uzbrojenia
Tematy:
validation
FOX-7
DADNE
HPLC method
assay
purity
walidacja
metoda HPLC
zawartość
Opis:
1,1-diamino-2,2 dinitroethylen (DADNE, FOX-7) is a novel explosive with low sensivity. The valuable properties is a result of specific chemical construction, in which inter- and intramolecular hydrogen bonds stabilize the structure. From the reason of low sensivity, FOX-7 has a very broad spectrum of use for elaborating many kinds of munnition. Hence a very important aspect is to conduct the research of this substance to determine chemical purity, assay content of water etc. The HPLC method is the one of the analytical technique which has a very high precision of determination of the purity and assay of the substance. The aim of this work was to compile a new method of the assay and purity determination of FOX-7. The C18-Xbridge column and the mobile phase: Acetonitryle: Water: Triethylamine were used. Through application of the C18- XBridge column and mobile phase the substrate MPD and main substance FOX-7 had very good resolution and the analysis time was relatively short. The validation of the new method has been performed: selectivity, specificity, linearity, precision, assay and limits of detection and quantitation. We have also checked the stress tests of the FOX-7 in acidic, basic and oxidative conditions.
1,1-diamino-2,2 dinitroetylen (DADNE, FOX-7) jest nowoczesną małowrażliwą substancją wybuchową. Jej cenne właściwości są rezultatem specyficznej budowy chemicznej, w której wewnątrz i zewnątrz cząsteczkowe wiązania wodorowe stabilizują strukturę, powodując jej małą wrażliwość na bodźce. Dzięki takim właściwościom, FOX-7 może być zastosowany do elaboracji wielu rodzajów małowrażliwej amunicji. Dlatego tak ważnym aspektem jest prowadzenie badań chemicznych uwzględniających czystość i zawartość chemiczną tej substancji. Metoda HPLC jest nowoczesną techniką, szeroko stosowaną do określenia tych parametrów z dużą dokładnością i precyzją. Celem naszej pracy było zwalidowanie nowej metody oznaczania czystości i zawartości FOX-7. Do oznaczania tych parametrów użyto kolumny C-18 X-Bridge natomiast jako fazy ruchomej mieszaniny acetonitrylu trietyloaminy: wody zmieszanej w odpowiednim stosunku. Zastosowanie właśnie takiej kolumny oraz fazy z modyfikatorem pozwoliło na podzielenie zanieczyszczenia MPD od substancji głównej w odpowiednio krótkim czasie z dobrą rozdzielczością oraz symetrią pików. Walidacja metody obejmowała: selektywność, specyficzność, precyzję, dokładność oraz limity detekcji oraz oznaczalności. Podczas walidacji zostały również przeprowadzone testy stresowe w warunkach kwaśnych, zasadowych oraz utleniających.
Źródło:
Problemy Techniki Uzbrojenia; 2014, 43, 130; 79-87
1230-3801
Pojawia się w:
Problemy Techniki Uzbrojenia
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Nitrate Reductase Activity in Eucalyptus urophylla and Khaya senegalensis Seedlings: Optimization of the in vivo Assay
Autorzy:
Barbosa, Mateus Pires
do Bonfim, Raul Antonio Araújo
da Silva, Leandro Dias
Souza, Mikaela Oliveira
de Souza Soares, Poliana Prates
Sá, Milton Carriço
Cairo, Paulo Araquém Ramos
Powiązania:
https://bibliotekanauki.pl/articles/2027823.pdf
Data publikacji:
2022
Wydawca:
Polskie Towarzystwo Inżynierii Ekologicznej
Tematy:
nitrogen assimilation
enzymatic activity
in vivo assay
woody plant
Opis:
Nitrate assimilation in the plant cell is mainly regulated by the enzyme nitrate reductase (EC 1.6.6.1), which catalyzes the nitrate to nitrite reduction. Nitrate reductase activity (NRA) is measured at the major nitrate reduction site, which can be the root or shoot, depending on the species. The in vivo assay has often been used for NRA measurement, and protocol also usually varies with the species. The goals of this study were: (1) to identify the major nitrate reduction site in seedlings of two tree species, Eucalyptus urophylla and Khaya senegalensis, and (2) to optimize the in vivo nitrate reductase assay at the major nitrate reduction site in these species. Healthy seedlings aged 180 and 160 days, respectively, were selected for NRA measurement in fully expanded leaves and main root. After identifying the main nitrate reduction site of each species, the effects of variations in temperature, nitrate concentration and pH in the incubation medium were assessed. The results showed that the leaf and the root are the major nitrate reduction site of Eucalyptus urophylla and Khaya senegalensis, respectively. The optimal conditions for the in vivo assay in the leaf were 35 °C, $KNO_3$ 100 mM, and pH 7.0, whereas for the root they were 30 °C, $KNO_3$ 100 mM, and pH 7.5.
Źródło:
Journal of Ecological Engineering; 2022, 23, 2; 204-211
2299-8993
Pojawia się w:
Journal of Ecological Engineering
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Synthesis and anticancer activity evaluation of novel derivatives of 7-amino-4-methylquinolin-2(1H)-one
Autorzy:
Kubica, Krzysztof P.
Taciak, Przemyslaw P.
Czajkowska, Agnieszka
Sztokfisz-Ignasiak, Alicja
Wyrebiak, Rafal
Mlynarczuk-Bialy, Izabela
Malejczyk, Jacek
Mazurek, Aleksander P.
Powiązania:
https://bibliotekanauki.pl/articles/895717.pdf
Data publikacji:
2018-08-31
Wydawca:
Polskie Towarzystwo Farmaceutyczne
Tematy:
anticancer activity
cytotoxicity assay
cell migration assay
quinolin-2(1H)-one
Opis:
In this study we designed and synthesized sixteen new derivatives of 7-amino-4-methylquinolin-2(1H)-one with potential anticancer activity. The structures of synthesized compounds were confirmed by 1H and 13C NMR. The activity of novel substances was evaluated by cell viability assay and wound healing assay. In vitro tests for series of sixteen novel compounds were performed. The results showed that examined compounds are selective for cancer cells, but their activity for various types of cancer is different. Three of new compounds presented ability to inhibit cells migration. The novel compounds constitute a good starting point for further studies and optimization of structure for new therapeutically effective anti-cancerous drugs. Seven compounds, which showed the highest rate of cell inhibition, were selected for further studies.
Źródło:
Acta Poloniae Pharmaceutica - Drug Research; 2018, 75, 4; 903-910
0001-6837
2353-5288
Pojawia się w:
Acta Poloniae Pharmaceutica - Drug Research
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Enzymatic assay of polyenoic fatty acids contents in blood plasma lipids
Autorzy:
Bulhak-Jachymczyk, B.
Powiązania:
https://bibliotekanauki.pl/articles/1371725.pdf
Data publikacji:
1992
Wydawca:
Instytut Rozrodu Zwierząt i Badań Żywności Polskiej Akademii Nauk w Olsztynie
Tematy:
blood plasma
enzymatic method
lipid
polyenoic fatty acid
lipoxygenase
Źródło:
Polish Journal of Food and Nutrition Sciences; 1992, 01, 1; 81-87
1230-0322
2083-6007
Pojawia się w:
Polish Journal of Food and Nutrition Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Probiernictwo w czasie pandemii
The hallmarking during the time of pandemic
Autorzy:
Ulaczyk, Maria Magdalena
Powiązania:
https://bibliotekanauki.pl/articles/88777.pdf
Data publikacji:
2020
Wydawca:
Główny Urząd Miar
Tematy:
probiernictwo
pandemia
urzędy probiercze
hallmarking
pandemic
assay offices
Opis:
W artykule omówiono problemy, jakie w powstały w urzędach probierczych w związku z panującą pandemią oraz jej negatywne skutki.
The article presents the problems that arose in assay offices in connection with the prevailing pandemic and its negative effects.
Źródło:
Metrologia i Probiernictwo : biuletyn Głównego Urzędu Miar; 2020, 1 (24); 45-49
2300-8806
Pojawia się w:
Metrologia i Probiernictwo : biuletyn Głównego Urzędu Miar
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Insect antifeedant potent quinoxalines
Autorzy:
Lakshmanan, K.
Sekar, K. G.
Sathiyendiran, V.
Muthuvel, I.
Powiązania:
https://bibliotekanauki.pl/articles/412501.pdf
Data publikacji:
2015
Wydawca:
Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
Tematy:
6-substituted aryl qyinoxalines
leaf-disc bio assay
Insect antifeedant activity
Opis:
Some 6-substituted aryl quinoxalines have been prepared and their purities were checked by their physical constants and spectroscopic data reported earlier. The insect antifeedant activities of these quinolones were studied by leaf-discs bio assay method using 4th instar larvae Achoea Janata L.
Źródło:
International Letters of Chemistry, Physics and Astronomy; 2015, 41; 82-87
2299-3843
Pojawia się w:
International Letters of Chemistry, Physics and Astronomy
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Współpraca międzynarodowa w dziedzinie badania i cechowania artykułów z metali szlachetnych
Autorzy:
Mikiel, M.
Powiązania:
https://bibliotekanauki.pl/articles/1425564.pdf
Data publikacji:
2014
Wydawca:
Główny Urząd Miar
Tematy:
urzędy probiercze
współpraca
historia probiernictwa
assay offices
cooperation
history of hallmarking
Opis:
Artykuł omawia współpracę międzynarodową polskich urzędów probierczych. Prezentuje poszczególne międzynarodowe organizacje probiercze, przyczynę ich powstania, strukturę i zakres działania. W artykule opisane zostały także korzyści, jakie odnosi nasz kraj dzięki przynależności do tych organizacji. Treść uzupełnia, przedstawiony w skrócie, zarys historii probiernictwa.
The article tells about the international cooperation of the polish assay offices. It presents international hallmarking organizations, its history, structure, terms of reference. Emphasizes benefits that our country receives due to the participation in these organizations as well as some short sketch of the history of hallmarking.
Źródło:
Metrologia i Probiernictwo : biuletyn Głównego Urzędu Miar; 2014, 4 (7); 40--42
2300-8806
Pojawia się w:
Metrologia i Probiernictwo : biuletyn Głównego Urzędu Miar
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Enzymatic assay of opioid analgesic, tramadol, using horseradish peroxidase
Autorzy:
Sridevi, N.
Srilakshmi, C.
Alekya, G.
Bala Sekaran, C.
Powiązania:
https://bibliotekanauki.pl/articles/10767.pdf
Data publikacji:
2014
Wydawca:
Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
Tematy:
opioid
tramadol
horseradish peroxidase
enzymatic method
Opis:
Three novel and sensitive enzymatic methods have been developed for the quantification of tramadol in pharmaceutical dosage forms. The proposed methods are based on the reaction of tramadol with 3-methylbenzothiazoline-2-one hydrazone (method A), aniline (method B) and aminoantipyrine in the presence of hydrogen peroxide and horseradish peroxidase to give colored complexes. The colored complexes obtained with 3-methylbenzothiazoline-2-one hydrazone, aniline and aminoantipyrine exhibit absorption maxima at 480 nm, 550 nm and 530 nm, respectively. Regression analysis of Beer’s plots showed good correlation for tramadol in the concentration range (μg/mL) 2-12 for the methods A & B and 4-24 for the method C. The experimental parameters were studied and optimized. The precision and accuracy of the methods were satisfactory. The proposed methods were successfully applied for the quantification of tramadol in 3 brands of commercially available tablet dosage forms. The results were compared satisfactorily with the official method.
Źródło:
International Letters of Natural Sciences; 2014, 11, 1
2300-9675
Pojawia się w:
International Letters of Natural Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Zastosowanie spektrofotofluorymetrii do oznaczeń katecholamin w moczu
Primenenie spektrofotofluorimetrii dlja opredelenija katekholaminov v moche
Spectrofluorometry in assay of urinary catecholamines
Autorzy:
Maruchin, J.
Olesiński, J.
Powiązania:
https://bibliotekanauki.pl/articles/2188676.pdf
Data publikacji:
1978
Wydawca:
Polskie Towarzystwo Diagnostyki Laboratoryjnej
Źródło:
Diagnostyka Laboratoryjna; 1978, 14, 5; 287-295
0867-4043
Pojawia się w:
Diagnostyka Laboratoryjna
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Wybrane metody poprawy wyników segmentacji obiektów w postaci skupisk na przykładzie obrazów kometowych
Selected methods for improvement of image segmentation of cluster-structured objects on an example of comet assay images
Autorzy:
Bal, A.
Powiązania:
https://bibliotekanauki.pl/articles/158425.pdf
Data publikacji:
2014
Wydawca:
Stowarzyszenie Inżynierów i Techników Mechaników Polskich
Tematy:
analiza obrazów
segmentacja obrazów
binaryzacja obrazu
filtracja obrazów
operacje morfologiczne
obrazy kometowe
image analysis
image segmentation
image binarization
image filtering
morphological operations
comet assay images
Opis:
W pracy przedstawiono metody poprawy wyników segmentacji obiektów w postaci skupisk. Poprawa jakości segmentacji dla takich obiektów może być uzyskana m.in. poprzez odpowiednie połączenie filtracji dolnoprzepustowej (np. filtracji medianowej) z metodami pozwalającymi na określenie przynależności poszczególnych obszarów uzyskanych po segmentacji wybraną metodą do obszarów wynikowych reprezentujących obiekty interesujące z punktu widzenia celu segmentacji (w pracy zastosowano w tym celu operację morfologicznego zamknięcia oraz metodę grafową wykorzystującą koncepcję minimalnego drzewa rozpinającego). W pracy zwrócono także uwagę na możliwość poprawy wyników segmentacji poprzez wyznaczenie otoczki wypukłej rozpiętej na obszarach spełniających określone wymagania. Wyniki działania opisanych metod przedstawiono na przykładzie poprawy wyników segmentacji obrazów otrzymanych w wyniku jednokomórkowej elektroforezy żelowej.
This paper deals with the problem of segmentation of cluster-structured objects, that is the objects which are formed by a set of unconnected elements smaller than the object. Images representing such objects are very difficult for segmentation. A good example of cluster-structured objects are “comet” images from Single Cell Gel Electrophoresis (comet assay). In the analysis of comet assay images a pivotal role plays the detection of comet regions - the comet region is formed by unconnected fragments of DNA (Fig. 1) which originate from the same cell nucleus. Because those regions are not solid, the usage of standard segmentation methods leads to poor results (Fig. 2). Pre- and post-processing methods can be used for improvement of segmentation results. Some of them are presented in the paper. The aim of the work was not the selection of the best improvement method of the comet assay segmentation results but the presentation of different approaches which could be used in the case of segmentation of this kind of objects. The first presented method is based on the idea of artificial removing of connectivity lack - this is done by the usage of a low-pass filter with large window before segmentation (Fig. 3). The next method uses the morphological closing for assignment of regions ri to the metaregion Rk which represent one comet (Fig. 4). For improving Rk shape the idea of convex hull spanned on ri ∈ Rk is used (Fig. 5). In this method the assignment condition is enclosed in structuring elements - because of limitations of the discrete space, in which the structuring element is defined, there are also some limitations of conditions which can be used. This drawback does not exist in the last of the presented method which uses the conception of the minimum spanning tree for assignment of regions ri to Rk. In this method the segmentation result is represented by graph G, whose vertexes vi represent regions ri, and length dij of edge eij between vertexes vi, and vj is equal to the closest distance between pixels of ri and rj (distances dij in general case can be variously defined). In G the minimum spanning trees Tk are searched, such that ∀eij ∈ Tk : dij ≤ ε, then for each Tk its convex hull is created - it defines the region of comet Kp (Fig. 6).
Źródło:
Pomiary Automatyka Kontrola; 2014, R. 60, nr 5, 5; 288-289
0032-4140
Pojawia się w:
Pomiary Automatyka Kontrola
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
DNA damage in subpopulations of human lymphocytes irradiated with doses in the range of 0-1 Gy of X-radiation
Autorzy:
Wojewódzka, M.
Machaj, E. K.
Goździk, A.
Iwaneńko, T.
Ołdak, T.
Kruszewski, M.
Pojda, Z.
Powiązania:
https://bibliotekanauki.pl/articles/147760.pdf
Data publikacji:
2008
Wydawca:
Instytut Chemii i Techniki Jądrowej
Tematy:
alkaline comet assay
biodosimetry
micronucleus test
histone gammaH2AX foci immunofluorescence assay
T lymphocytes
B lymphocytes
ionizing radiation
DNA damage
Opis:
We compared three methods usually applied in biological dosimetry for estimation of radiation-induced DNA damage in human T and B lymphocytes: alkaline comet assay, micronucleus (MN) test and formation of histone gamma-H2AX foci. Human peripheral blood lymphocytes were fractionated using T cells and B cells isolation kits. Cells were irradiated with doses in the range of 0-1 Gy of X-rays. Induction of DNA damage was assessed by the standard alkaline comet assay, MN test and histone gammaH2AX foci immunofluorescence assay. Notwithstanding different end-points measured by the applied methods, all tests revealed a similar induction of DNA damage in B lymphocytes as compared with T lymphocytes. The results indicated that all three tests detect DNA damage with similar sensitivity, the lowest dose being approximately 0.3 Gy. The difference between irradiated and control cells was expressed as the ratio of the value obtained for irradiated cells (1 Gy) to that for control cells. The highest ratio was obtained for formation of gammaH2AX foci and was 6.2 for T and 13.8 for B lymphocytes, whereas those for comet assay and micronucleus test were 3.5; 3.6 and 5.6; 4.8, respectively.
Źródło:
Nukleonika; 2008, 53, 4; 145-149
0029-5922
1508-5791
Pojawia się w:
Nukleonika
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Ocena cytotoksyczności kopolimeru glikolidu z laktydem (PLGA) w warunkach in vitro
Cytotoxicity of polylactide-co-glycolide (PLGA) - evaluation in vitro
Autorzy:
Cieślik, M.
Król, W.
Mertas, A.
Morawska-Chochół, A.
Ziąbka, M.
Chłopek, J.
Powiązania:
https://bibliotekanauki.pl/articles/284968.pdf
Data publikacji:
2008
Wydawca:
Akademia Górniczo-Hutnicza im. Stanisława Staszica w Krakowie. Polskie Towarzystwo Biominerałów
Tematy:
biomateriały
kopolimer PLGA
cytotoksyczność
test MTT
test LDH
osteoblasty ludzkie
badania biologiczne
biomaterials
copolymer PLGA
cytotoxicity
MTT assay
LDH assay
human osteoblasts
biological evaluations
Opis:
Celem pracy była ocena in vitro cytotoksycznego działania bioresorbowalnego kopolimeru glikolidu z laktydem (PLGA) na ludzkie osteoblasty linii hFOB 1.19 poprzez pomiar aktywności dehydrogenazy mitochondrialnej (test MTT) oraz dehydrogenazy mleczanowej (test LDH). Do badań użyto ekstrakt uzyskany po 8 dniach inkubacji kopolimeru PLGA w medium wykorzystywanym do hodowli osteoblastów. Ekstrakt ten następnie kontaktowano przez 24 oraz 48 godziny z zaadherowanymi do dna naczynia hodowlanego osteoblastami. Po upływie założonego czasu inkubacji zarówno test MTT, jak i test LDH nie wykazał cytotoksycznego działania kopolimeru PLGA na ludzkie komórki kościotwórcze.
The aim of the work was to evaluate in vitro the cytotoxic effect of bioresorbable polylactide-co-glycolide (PLGA) on the hFOB 1.19 human osteoblastic cell line by measuring the activity of mitochondrial dehydrogenase (MTT test) and lactate dehydrogenase (LDH test). The research made use of an extract obtained after 8 days of PLGA incubation in a medium used for osteoblast culturing. The extract was then brought into contact with osteoblasts adhered to the bottom of the culture vessel for 24 and 48 hours. After the set incubation time neither the MTT test nor the LDH test showed a cytotoxic effect of PLGA on human osteogenic cells.
Źródło:
Engineering of Biomaterials; 2008, 11, no. 81-84; 35-39
1429-7248
Pojawia się w:
Engineering of Biomaterials
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Synthesis and anticancer activity evaluation of some new derivatives of 2-(4-benzoyl-1-piperazinyl)-quinoline and 2-(4-cinnamoyl-1-piperazinyl)-quinoline.
Autorzy:
Kubica, Krzysztof P.
Taciak, Przemyslaw P.
Czajkowska, Agnieszka
Sztokfisz-Ignasiak, Alicja
Wyrebiak, Rafal
Podsadni, Piotr
Mlynarczuk-Bialy, Izabela
Malejczyk, Jacek
Mazurek, Aleksander P.
Powiązania:
https://bibliotekanauki.pl/articles/895388.pdf
Data publikacji:
2018-08-31
Wydawca:
Polskie Towarzystwo Farmaceutyczne
Tematy:
anticancer activity
cytotoxicity assay
2-quinoline
cell migration assay
Opis:
In this study we designed and synthesized twenty new derivatives of 2-(4-benzoyl-1-piperazinyl)-quinoline and 2-(4-cinnamoyl-1-piperazinyl)-quinoline with potential anticancer activity. The structures of synthesized compounds were confirmed by 1H and 13C NMR spectroscopy and MS spectrometry. The activity of novel compounds was evaluated in the cell viability assay as well as in the wound healing assay. Presented data show that examined substances have anticancer activity in cell culture. Seven compounds which showed a high rate of cell growth inhibition were selected for further studies. Three of them strongly reduced growth of B16F10 cells. The novel compounds constitute a good base for further studies and optimization of structure for new therapeutically effective anti-cancerous drugs.
Źródło:
Acta Poloniae Pharmaceutica - Drug Research; 2018, 75, 4; 891-901
0001-6837
2353-5288
Pojawia się w:
Acta Poloniae Pharmaceutica - Drug Research
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Grafowa metoda segmentacji obiektów w postaci skupisk na przykładzie obrazów kometowych
Graph-based aggregate object image segmentation method on example of comet assay images
Autorzy:
Bal, A.
Powiązania:
https://bibliotekanauki.pl/articles/154889.pdf
Data publikacji:
2012
Wydawca:
Stowarzyszenie Inżynierów i Techników Mechaników Polskich
Tematy:
analiza obrazów
obiekty w postaci skupisk
minimalne drzewo rozpinające
test kometowy
jednokomórkowa elektroforeza żelowa
image analysis
image segmentation
aggregate objects
minimum spanning tree
comet assay
single cell gel electrophoresis
Opis:
W pracy przedstawiono propozycję metody segmentacji obiektów będących skupiskami - przykładem takich obiektów są tzw. komety, które są wynikiem jednokomórkowej elektroforezy żelowej. Opracowana metoda działa dwuetapowo: etap 1. to segmentacja służąca wyznaczeniu elementów składowych należących do obiektów, etap 2 wykorzystuje minimalne drzewo rozpinające do określenia zbioru elementów tworzących poszczególne obiekty, obszar poszczególnych obiektów wyznaczany jest jako otoczka wypukła odpowiedniego drzewa rozpinającego.
This paper deals with a problem of segmentation of aggregate objects, that is objects which are formed by a set of unconnected elements smaller than the object itself. Images of such a type of objects are very difficult for segmentation. An example of this type of objects are "comets" (Fig. 1, left column) from Single Cell Gel Electrophoresis images (also called comet assay images). In comet assay images the comet region is formed by unconnected fragments of DNA. Because of not satisfying results of comet segmentation with use of the standard methods, a new method for segmentation of such images was developed. The new method works in two stages. The first stage is the image segmentation-for comets the Bernsen binarization method (Eqs. (1) and (2)) with median filtering of the obtained results was chosen-the result of this stage is a set of comet elements ei which represent DNA fragments (Fig. 1, the 2nd column). In the second stage the minimum spanning trees Tp are created (Fig. 1, 3th column)-graph vertexes vi represent elements ei, and length dij of edge eij between vertexes vi and vj is equal to the closest distance between pixels of elements ei and ej-then for each connected tree Tp its convex hull which defines the region of comet Kp (Fig. 1, the 4th column) is created. In case of defects appearing in comet images, the incorrect region can be rejected e.g. by use of geometrical or photometrical features of the regions.
Źródło:
Pomiary Automatyka Kontrola; 2012, R. 58, nr 4, 4; 313-315
0032-4140
Pojawia się w:
Pomiary Automatyka Kontrola
Dostawca treści:
Biblioteka Nauki
Artykuł
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