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Wyświetlanie 1-10 z 238
Tytuł:
Grupa Wyszehradzka urzędów probierczych. Urząd Probierczy w Budapeszcie
Visegrad Group of Assay Offices. Assay Office in Budapest
Autorzy:
Ulaczyk, Maria Magdalena
Powiązania:
https://bibliotekanauki.pl/articles/2200076.pdf
Data publikacji:
2021
Wydawca:
Główny Urząd Miar
Tematy:
probiernictwo
Grupa Wyszehradzka
Urząd Probierczy w Budapeszcie
assay
Visegrad Group
Assay Office in Budapest
Opis:
Artykuł informuje o współpracy urzędów probierczych, prowadzonej w ramach Grupy Wyszehradzkiej oraz przedstawia, wizytowany niedawno przez pracowników OUP w Warszawie, Urząd Probierczy w Budapeszcie. W artykule wskazano jego specyfikę i odrębności w odniesieniu do polskich urzędów probierczych.
The article informs about the cooperation between assay offices carried out within the Visegrad Group and presents the recently visited by employees of Warsaw Assay Office, Assay Office in Budapest. The article shows its specific and distinctiveness, in comparison to polish assay offices.
Źródło:
Metrologia i Probiernictwo : biuletyn Głównego Urzędu Miar; 2021, 2 (27); 49--58
2300-8806
Pojawia się w:
Metrologia i Probiernictwo : biuletyn Głównego Urzędu Miar
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Identification of sterile wild oat (Avena sterilis L.) resistance to acetolactate synthase (ALS)-inhibiting herbicides using different assay techniques
Autorzy:
Abdurruhman, A.M.
Uygur, S.
Mathiassen, S.K.
Uygur, N.
Powiązania:
https://bibliotekanauki.pl/articles/2082753.pdf
Data publikacji:
2020
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
acetolactate synthase
agar-based assay
Avena sterilis
Petri-dish assay
whole-plant assay
Opis:
Different techniques have been devised to detect herbicide resistance in weeds, and the over- all aim from this study was to compare four different assay techniques for evaluating acetol- actate synthase (ALS)-inhibiting herbicide resistance in sterile wild oat (Avena sterilis L.). A resistant sterile wild oat population (R) was collected from the wheat field in Kozan, Adana province, Turkey. The susceptible (S) population was collected from the border of the same field. Effects of different doses of mesosulfuron-methyl + iodosulfuron-methyl- -sodium and pyroxsulam + cloquintocet-mexyl were assessed in agar based (seed and seed- ling) assay, Petri dish with seeds, and whole plant pot assay. In the agar based assays, the level of resistance was evaluated by measuring coleoptile and hypocotyl lengths, and sur- vival of seedlings. Plant height and shoot dry weight were measured in the Petri dish and whole plant pot assays, respectively. Results from the dose response analyses showed that both the R and S populations were extremely sensitive to mesosulfuron-methyl + iodosul- furon in the seedling bioassay. The resistance indices (RI’s) of the R biotype treated with mesosulfuron-methyl + iodosulfuron in the agar based seed, Petri dish, and whole plant assays were 2.29, 2.63 and 4.18, respectively. The resistance indices of the R biotype treated with pyroxsulam + cloquintocet-mexyl was 3.41, 5.05 and 2.82 in the agar based seed, Petri dish, and whole plant pot assays, respectively. The agar based seed assays and Petri dish as- say provided feasible, accurate, rapid, and cost effective opportunities to identify resistance in sterile wild oat.
Źródło:
Journal of Plant Protection Research; 2020, 60, 3; 244-252
1427-4345
Pojawia się w:
Journal of Plant Protection Research
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Modified neutral comet assay for human lymphocytes
Autorzy:
Wojewódzka, M.
Grądzka, I.
Buraczewska, I.
Powiązania:
https://bibliotekanauki.pl/articles/147130.pdf
Data publikacji:
2002
Wydawca:
Instytut Chemii i Techniki Jądrowej
Tematy:
DNA double strand breaks
human lymphocyte
neutral comet assay
Opis:
Comet assay under neutral conditions allows the detection of DNA double-strand breaks, considered to be the biologically relevant radiation-induced lesion. In this report we describe modifications of the neutral comet method, which simplify and facilitate its use for estimation of DNA double strand breaks in human lymphocytes irradiated with doses of 60Co gamma-rays (from 10 to 100 Gy). The analysis carried out according to this protocol takes less time than those published so far. Also, the use of lysis at 50°C is avoided; this is important in view of the presence of heat-labile sites in the DNA of irradiated cells, recently reported by Rydberg [12]. The comets have well defined, sharp limits, are suitable for computer image analysis and chromatin of the control cells remains condensed.
Źródło:
Nukleonika; 2002, 47, 1; 1-5
0029-5922
1508-5791
Pojawia się w:
Nukleonika
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
In vitro evaluation of zearalenone toxicity by comet assay
Autorzy:
Harcarova, M.
Conkova, E.
Kolenicova, S.
Holeckova, B.
Proskovcova, M.
Powiązania:
https://bibliotekanauki.pl/articles/16539336.pdf
Data publikacji:
2022
Wydawca:
Polska Akademia Nauk. Czasopisma i Monografie PAN
Tematy:
mycotoxins
toxicity
comet assay
Opis:
The aim of this study was to reveal the potentially genotoxic effect of zearalenone on bovine lymphocytes by comet assay in vitro. The bovine lymphocytes were exposed to various zearalenone concetrations (50; 10; 2; 0.4 and 0.08 ppm). The viability and DNA damage of lymphocytes was monitored after 2 h, 24 h, 48 h and 72 h. After 2 hours of zearalenone exposure, statistically significant DNA damage occurred at all tested concentrations of 0.08 ppm (12.2±1.25; p<0.05), 0.4 ppm (12.7±0.88; p<0.01), 2 ppm (12.0±0.51; p<0.01), 10 ppm (11.2±0.47; p<0.01) and at 50 ppm (14.2±0 61; p<0.001). Significantly greater DNA damage was also found after 24 h, 48 h and 72 h. The obtained results showed that zearalenone may induce DNA damage of the bovine lymphocytes.
Źródło:
Polish Journal of Veterinary Sciences; 2022, 25, 3; 475-477
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Detection of alkylation damage in human lymphocyte DNA with the comet assay.
Autorzy:
Collins, Andrew
Dušinská, Mária
Horská, Alexandra
Powiązania:
https://bibliotekanauki.pl/articles/1044089.pdf
Data publikacji:
2001
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
AlkA
DNA damage
comet assay
Opis:
The enzyme 3-methyladenine DNA glycosylase II (AlkA) is a bacterial repair enzyme that acts preferentially at 3-methyladenine residues in DNA, releasing the damaged base. The resulting baseless sugars are alkali-labile, and under the conditions of the alkaline comet assay (single cell gel electrophoresis) they appear as DNA strand breaks. AlkA is not lesion-specific, but has a low activity even with undamaged bases. We have tested the enzyme at different concentrations to find conditions that maximise detection of alkylated bases with minimal attack on normal, undamaged DNA. AlkA detects damage in the DNA of cells treated with low concentrations of methyl methanesulphonate. We also find low background levels of alkylated bases in normal human lymphocytes.Single cell gel electrophoresis (the comet assay) is widely used for the detection of strand breaks in nuclear DNA. It is particularly appropriate for studying the low background levels of damage present in normal human cells, such as peripheral lymphocytes. The cells are embedded in agarose on a microscope slide and lysed with Triton X-100 and 2.5 M NaCl, which remove cytoplasm and most nuclear proteins, but leave the DNA, in supercoiled form, as nucleoids. After incubation in alkali, the DNA is electrophoresed at high pH; DNA is drawn out to form a 'tail' (hence the name 'comet assay') - but only if breaks are present to relax the supercoiling of the nucleoid DNA. In order to increase its sensitivity and selectivity, we have incorporated into the assay an extra step in which the nucleoid DNA is digested with a lesion-specific endonuclease; the additional breaks revealed with this procedure indicate the presence of the particular lesion. So far, endonuclease III (NTH, specific for oxidised pyrimidines) (Collins et al., 1993), formamidopyrimidine DNA glycosylase (FPG, acting on ring-opened purines and the major purine oxidation produce, 8-oxoguanine) (Dušinská & Collins, 1996) and T4 endonuclease V (recognising UV-induced cyclobutane pyrimidine dimers) (Collins et al., 1997b) have been successfully employed. Amongst other things, we have estimated background levels of DNA oxidation (Collins et al., 1997a), and have found this damage to be elevated in human diseases such as diabetes and ankylosing spondylitis (Dušinská et al., 1999).We now report the use of AlkA, a bacterial repair enzyme whose main substrate is 3-methyladenine in DNA, though it also recognises - with lower efficiency - other modified bases (Lindahl, 1993). A recent report (Berdal et al., 1998) suggests that repair enzymes supposedly specific for alkylated bases may in fact create breaks non-selectively (though much less efficiently) at normal bases. Given the size of the genome, even a low efficiency of non-specific breakage could significantly interfere in estimations of background levels of alkylation damage. We reasoned that, by employing a range of concentrations of the enzyme, and carrying out incubations for different lengths of time, we might find a concentration at which only the alkylated bases would be detected, so that the number of breaks would increase to a certain level and then plateau. After optimising reaction conditions, we tested the assay on lymphocytes from different individuals, and also, as a positive control, examined alkylation damage induced by methyl methanesulphonate.
Źródło:
Acta Biochimica Polonica; 2001, 48, 3; 611-614
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Test mikrojądrowy w retrospektywnej dozymetrii biologicznej
Micronucleus assay in rapid retrospective biological dosimetry
Autorzy:
Rawojć, K.
Miszczyk, J.
Powiązania:
https://bibliotekanauki.pl/articles/134746.pdf
Data publikacji:
2016
Wydawca:
ADVSEO
Tematy:
micronuclei
micronucleus assay
retrospective biological dosimetry
ionizing radiation
cell death
Opis:
Rapid retrospective biological dosimetry allows absorbed dose evaluation post exposure to ionizing radiation. One of the main tools of biodosimetry is based on the analysis of the effects resulting from the impact of ionizing radiation on the cell. Various cytogenetic tests give possibility of the accurate dose estimation. To investigate cell response to radiation one performs the analysis of biomarkers approved by International Atomic Energy Agency e.g. the analysis of dicentric chromosomes or micronuclei frequency. Micronucleus test is relatively a faster and therefore more effective method to study changes in the genetic material, induced by various genotoxic agents. This study confirms that micronulei frequency and nuclear division index analysis allows for appropriate absorbed dose estimation when it comes to ionizing radiation. In order to further optimize and facilitate the micronucleus assay and other cytogenetic tests in rapid retrospective biological dosimetry, the research are still ongoing.
Źródło:
Technical Issues; 2016, 4; 78-82
2392-3954
Pojawia się w:
Technical Issues
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The use of Allium cepa L. assay as bioindicator for the investigation of genotoxic effects of industrial waste water
Autorzy:
Rasgele, Pinar Goc
Powiązania:
https://bibliotekanauki.pl/articles/2073789.pdf
Data publikacji:
2021
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
Allium cepa assay
mitotic index
waste water
Opis:
In this study, genotoxic potential of industrial waste water (IWW) samples was investigated using Allium cepa assay. The root tips were treated with different IWW samples (A, B and C) for 48 hours. The effects of IWW on cytological effects were determined. It was found that all IWW samples significantly increased the percentage of total abnormality. Mitotic chromosomal abnormalities such as irregular metaphase, stickiness, c-mitosis, micronucleus, vagrant chromosomes and bridges were determined. Furthermore, a significant reduction for the mitotic index that is indicative of cellular toxicity was observed in root tips cells, which were treated with IWW samples. A. cepa assay can be used as useful tool for the detection of genotoxic and cytotoxic potential of IWWs.
Źródło:
Archives of Environmental Protection; 2021, 47, 4; 3--8
2083-4772
2083-4810
Pojawia się w:
Archives of Environmental Protection
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Aggressiveness of Fusarium langsethiae isolates towards wheat, barley and oats in an in vitro leaf assay.
Autorzy:
Opoku, Nelson
Back, Matthew
Edwards, Simon G.
Powiązania:
https://bibliotekanauki.pl/articles/2199610.pdf
Data publikacji:
2011-12-20
Wydawca:
Instytut Hodowli i Aklimatyzacji Roślin
Tematy:
aggressiveness
Fusarium langsethiae
HT-2
in vitro
leaf assay
T-2
trichothecenes
Opis:
Fusarium langsethiae has been identified as the primary producer of HT-2 and T-2 in European cereals. HT-2 and T-2 are considered as two of the most potent trichothecenes mycotoxins and a public health concern in Europe. There is currently no legislation on HT-2 and T-2, however, there is a discussion limit of 500 µg kg -1  in unprocessed oats, 200 µg kg-1 for oat products and 50 µg kg-1 for infant food. There are limited data regarding F. langsethiae’s pathogenicity and mycotoxin production, but it is evident that its behaviour deviates from traditional trichothecene-producing Fusarium species. This experiment was aimed at assessing the aggressiveness (measured by lesion length) of 20 different F. langsethiae isolates on wheat, barley and oats using an in vitro detached leaf assay. There was a significant (P<0.001) difference between lesion lengths formed by different F. langsethiae isolates used. Isolate Fl/2004/17(a) caused the shortest lesion on all cereals and this was significantly (P<0.001) different from that caused by isolate Fl/0/08/009/1 which caused the longest lesion on all cereals used. A highly significant difference (P<0.001) was also observed between lesions on the different cereals (wheat, barley and oats). Lesions on oats were the longest, followed by barley with wheat showing the shortest lesions.
Źródło:
Plant Breeding and Seed Science; 2011, 64; 55-64
1429-3862
2083-599X
Pojawia się w:
Plant Breeding and Seed Science
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Utworzenie Urzędu Probierczego w Krakowie i jego działalność w latach 1843–1853
Establishment of the Assay Office in Kraków and its activities in the years 1843–53
Autorzy:
Mataniak, Mateusz
Powiązania:
https://bibliotekanauki.pl/articles/965379.pdf
Data publikacji:
2016
Wydawca:
Krakowska Akademia im. Andrzeja Frycza Modrzewskiego
Tematy:
Free City of Kraków
Assay Office
Jewellers and Goldsmiths Guild
jewellery
assay of gold
Wolne Miasto Kraków
Urząd Probierczy
cech jubilerów i złotników
wyroby jubilerskie
próba karatowa
Opis:
The article presents the circumstances of setting up the Assay Office in the Free City of Kraków and also its activity in 1843–53. The source base consisted of regulations of the Governing Senate on the organisation and competence of the Assay Office, and documentation related to its activity, being a part of the complex of the Archive of the Free City of Kraków. The fundamental task of the Assay Office was to supervise Kraków jewellers and goldsmiths. The supervision especially focused on gold and silver objects brought for stamping. The office charged fees defined by the government for its services. The article also mentions prosecution of crimes against the assay stamp, which was a prerogative of the administrative authorities: the Senate, and later the Administrative Council and the City Council
W artykule przedstawiono okoliczności powstania Urzędu Probierczego w Wolnym Mieście Krakowie, a także przebieg jego działalności w latach 1843–1853. Podstawę źródłową stanowiły rozporządzenia Senatu Rządzącego dotyczące organizacji i kompetencji Urzędu Probierczego, a także związana z jego działalnością dokumentacja, stanowiąca część zespołu Archiwum Wolnego Miasta Krakowa. Podstawowym zadaniem Urzędu Probierczego było nadzorowanie krakowskich jubilerów i złotników, przy czym kontrola obejmowała zwłaszcza przynoszone przez nich do ostemplowania wyroby ze złota i srebra. Za swoje czynności urząd pobierał opłaty według taksy rządowej. W artykule wspomniano też o ściganiu przestępstw probierczych, co należało do władz administracyjnych: Senatu, a następnie Rady Administracyjnej i Rady Miejskiej.
Źródło:
Studia z Dziejów Państwa i Prawa Polskiego; 2016, 19; 179-198
1733-0335
Pojawia się w:
Studia z Dziejów Państwa i Prawa Polskiego
Dostawca treści:
Biblioteka Nauki
Artykuł
Wyświetlanie 1-10 z 238

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