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    Tytuł:
    The influence of different composite mixtures (PLA/HA) manufactured with additive laser technology on the ability of S. aureus and P. aeruginosa to form biofilms
    Autorzy:
    Woźna, A. E.
    Junka, A. F.
    Szymczyk, A. E.
    Powiązania:
    https://bibliotekanauki.pl/articles/306425.pdf
    Data publikacji:
    2018
    Wydawca:
    Politechnika Wrocławska. Oficyna Wydawnicza Politechniki Wrocławskiej
    Tematy:
    biomateriały
    biofilm
    biopolimery
    bioceramika
    biomaterials
    prototype additive manufacturing biopolymers
    bioceramic
    prototype additive manufacturing
    Opis:
    Staphylococcus aureus (Gram-positive coccus) and Pseudomonas aeruginosa (Gram-negative bacterium) are the leading etiologic agents of biofilm-related, life-threatening infections in patients after orthopaedic implantations. The aim of the present paper is to estimate the ability of these two bacterial strains to form a biofilm on bioresorbable composites manufactured from polylactide (PLA) and hydroxyapatite (HA) with the use of Selective Laser Sintering (SLS) method. Methods: Microbiological tests were conducted on two variants of a solid specimen made with additive laser technology. Samples with different content of hydroxyapatite were made, with appropriate manufacturing parameters to ensure stability of both composite ingredients. The geometry of samples was obtained by technical computed tomography. Microbiological tests determined the number of bacterial cells after incubation. Results: The results indicate significantly decreased ability of S. aureus and P. aeruginosa to form biofilms on the surface of materials with higher content of hydroxyapatite ceramics. Conclusions: The data may be useful for future applications of SLS technology in the production of bioresorbable PLA/HA medical implants
    Źródło:
    Acta of Bioengineering and Biomechanics; 2018, 20, 3; 101-106
    1509-409X
    2450-6303
    Pojawia się w:
    Acta of Bioengineering and Biomechanics
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Motility activity, slime production, biofilm formation and genetic typing by ERIC-PCR for Pseudomonas aeruginosa strains isolated from bovine and other sources (human and environment)
    Autorzy:
    Wolska, K.
    Szweda, P.
    Lada, K.
    Rytel, E.
    Gucwa, K.
    Kot, B.
    Piechota, M.
    Powiązania:
    https://bibliotekanauki.pl/articles/30220.pdf
    Data publikacji:
    2014
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Opis:
    The molecular-typing strategy, ERIC-PCR was used in an attempt to determine the genomic relationship of 28 P. aeruginosa strains isolated from faeces of healthy bovine, bovine mastitis and from faeces of hospital patients as well as from environment. ERIC-PCR fingerprinting revealed large molecular differentiation within this group of isolates. Twenty two out of 28 strains tested generated unique patterns of DNA bands and only three genotypes consisted of two isolates each were identified. We also tested the P. aeruginosa isolates for their ability to form a biofilm on abiotic surfaces including polyvinylchloride and polystyrene. Different biofilm-forming abilities were demonstrated among strains; however, most of them (64.3%) showed moderate-biofilm forming ability. The strains with increased swimming and twitching motility displayed elevated biofilm formation. However, a negative correlation was found between slime and initial biofilm production. On the basis of the results obtained, we suggest that there are no major differences in phenotypic properties between P. aeruginosa strains isolated from different sources.
    Źródło:
    Polish Journal of Veterinary Sciences; 2014, 17, 2; 321-329
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Analiza wstępowania szpitalnych zakażeń układu moczowego w 10-letniej obserwacji oddziału chorób wewnętrznych i nefrologii w Małopolsce
    Autorzy:
    Wałaszek, Marta
    Kołpa, Małgorzata
    Wolak, Zdzisław
    Sydor, Antoni
    Pelczar, Anna
    Powiązania:
    https://bibliotekanauki.pl/articles/2097675.pdf
    Data publikacji:
    2017-06-30
    Wydawca:
    Państwowa Wyższa Szkoła Zawodowa w Tarnowie
    Tematy:
    ddział chorób wewnętrznych
    zakażenie szpitalne
    Healthcare – Associated Infections (HAI)
    szpitalne zakażenie układu moczowego (UTI)
    E. coli
    P. aeruginosa
    internal medicine department
    hospital-acquired infections
    healthcare associated infections
    urinary tract infections
    Opis:
    Wstęp: Zakażenia szpitalne są jednym z poważniejszych zagrożeń zdrowia pacjenta podczas hospitalizacji. Wśród nich zakażenia szpitalne HAI (Healthcare – Associated Infections). Najczęściej występującą formą są szpitalne zakażenia układu moczowego (UTI – Urinary Tract Infection). Cel: W celu zbadania częstości występowania szpitalnych zakażeń układu moczowego poddano analizie strukturę zakażeń szpitalnych występujących u pacjentów hospitalizowanych w Oddziale Chorób Wewnętrznych i Nefrologii Szpitala Wojewódzkiego im. Św. Łukasza w Tarnowie. Materiał i metody: Analizowano dane dotyczące 13 965 pacjentów hospitalizowanych w Oddziale Chorób Wewnętrznych i Nefrologii w latach od 2006 do 2015. W analizie materiału wykorzystano standardowe metody epidemiologiczne i ujednolicone definicje zakażeń szpitalnych wydane przez ECDC (European Center for Disease Prevention and Control) oraz CDC (Centers for Disease Control and Prevention). Wyniki: Wykryto 237 szpitalnych UTI co stanowiło 33% wszystkich zakażeń szpitalnych wykrytych w badanym oddziale. Zachorowalność wynosiła 1,7%, w tym: 1,5% dla potwierdzonych mikrobiologicznie zakażeń (UTI-A: Microbiologically Confirmed Symptomatic UTI) i 0,2% bez potwierdzenia mikrobiologicznego (UTI-B: Not Microbiologically Confirmed Symptomatic UTI). Współczynnik gęstości zachorowań na 1000 osobodni pobytu (ID – density incidence /1000 osobodni pobytu) wynosił 0,2/1000. Rozpoznano 168 przypadków zakażeń UTI związanych z cewnikiem moczowym i 69 przypadków bez cewnika moczowego. Współczynnik gęstości zachorowań dla UTI z cewnikiem moczowym wynosił 3,3 na 1000 osobodni z cewnikiem moczowym. Wśród czynników etiologicznych, które izolowano z materiałów pochodzących od pacjentów z zakażeniem układu moczowego (UTI) dominowały: Escherichia coli 63 (29%), Enterococcus spp. 37 (16%), Klebsiella spp. 23 (11%). Wnioski: Dziesięcioletnia obserwacja szpitalnych UTI występujących w oddziale chorób wewnętrznych i nefrologii wykazała możliwość przeprowadzenia bardzo dokładnej analizy epidemiologicznej tych zakażeń. Porównanie gęstości występowania szpitalnych UTI uzyskane w badanym oddziale z odnotowaną w programach CDC pozwala wnioskować, iż sytuacja epidemiologiczna w badanym oddziale nie odbiega znacząco od sytuacji w innych krajach.
    Introduction: Hospital-acquired infections are one of the most serious health threats during a patient’s stay in hospital, including healthcare associated infections (HAI). The most typical form of hospital-acquired infections is urinary tract infection (UTI). Objective: To examine the frequency of appearing UTIs, the structure of UTIs in in-patients in the department of internal medicine and nephrology at Saint Lucas’s general hospital in Tarnów was analysed. Materials and methods: Data analysis of 13 965 in-patients staying in the department of internal medicine and nephrology from 2006 to 2015 was carried out. To investigate these data epidemiological methods and standard definitions of hospital- acquired infections issued by European Center for Disease Prevention as well as Control and Centers for Disease Control and Prevention were used. Results: 237 hospital-acquired UTIs were revealed, which is 33% of all UTIs revealed in the investigated ward. The UTI incidence rate was 1.7% including 1.5% for microbiologically confirmed symptomatic UTIs and 0.2% not microbiologically confirmed symptomatic UTIs. The incidence density rate per 1 000 person-days was 0.2 over 1 000. The number of revealed catheter-related cases was 168, and not catheter-related cases – 69. The incidence density rate of UTIs associated with urinary catheters was 3.3 per 1 000 person-days. The dominant etiological factors, which were taken to be detected from the infected patients’ specimens, were: Escherichia coli 63 (29%), Enterococcus spp. 37(16%), Klebsiella spp. 23 (11%). Conclusions: A 10 year observation of UTIs, which have appeared in the department of internal medicine and nephrology, allowed to conduct the accurate analysis of these infections. The comparison of urinary tract infection rates done in the investigated ward, and recorded in the Research Participation Programs at the Centers for Disease Control and Prevention (CDC), allows to draw the conclusion that the presented epidemiological situation does not differ significantly from other countries.
    Źródło:
    Health Promotion & Physical Activity; 2017, 2, 3; 141-154
    2544-9117
    Pojawia się w:
    Health Promotion & Physical Activity
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Screening of phytochemical profile and antibacterial activity of various solvent extracts of marine algae Sargassum swartzii
    Autorzy:
    Sujatha, Ravi
    Siva, Durairaj
    Nawas, P. Mohideen Askar
    Powiązania:
    https://bibliotekanauki.pl/articles/1076470.pdf
    Data publikacji:
    2019
    Wydawca:
    Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
    Tematy:
    Aeromonas hydrophila
    Antibacterial activity
    Pseudomonas aeruginosa
    Sargassum swartzii
    Total flavonoid
    Total phenol
    Opis:
    The present study investigates phytochemical screening and antibacterial efficacy of various solvent extracts of marine algae such as Sargassum swartzii against some selected human and fish pathogenic bacteria. The brown seaweed Sargassum swartzii was collected from Kudankulam, Tirunelveli district, Tamil Nadu, India. Soxhlet extraction method was used to get the extracts of different solvents like aqueous, ethanol, methanol, acetone and were tested for their qualitative phytochemical screening using common standard tests. Quantitative phytochemical analysis such as total phenolic content and total flavonoid content of S. swartzii and antibacterial activity against human pathogens viz., Escherichia coli, Pseudomonas aeruginosa and Stephylococcus aureus and fish pathogens viz., Aeromonas hydrophila and Vibrio vulnificus bacteria using disc diffusion methods. Preliminary phytochemical screening of sixteen different chemical compounds were carried out. The maximum biochemical compounds were present in the ethanol extract and the minimum compounds were present in aqueous extract. The ethanol extract was significantly higher in phenolic content (15.35 ±2.61 mg of GAE/g) and the methanol extract was significantly higher in flavonoides content (26.92±7.14 mg of QE/g). In human pathogen the highest antibacterial activity was present in Pseudomonas aeruginosa (18.00±0.00mm) and the fish pathogen was significantly higher in Aeromonas hydrophila. The present study showed that the ethanol extract of marine brown seaweed Sargassum swartzii contains bio active constituents with highest antibacterial activity against human and fish pathogen.
    Źródło:
    World Scientific News; 2019, 115; 27-40
    2392-2192
    Pojawia się w:
    World Scientific News
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Isolation of bacteria from soil sample of Tamil Nadu and their in vitro interaction
    Autorzy:
    Sankari, D.
    Khusro, Ameer
    Aarti, Chirom
    Powiązania:
    https://bibliotekanauki.pl/articles/1031683.pdf
    Data publikacji:
    2020
    Wydawca:
    Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
    Tematy:
    Antagonism
    B. subtilis
    CFU
    E. coli
    Interaction
    P. aeruginosa
    Opis:
    The present study was investigated to determine the competitive interaction between Gram positive (B. subtilis) and Gram negative (P. aeruginosa and E. coli) bacteria in vitro. Bacteria of interest were isolated from soil sample and were identified through morphological and biochemical tests. Bacterial cultures were grown alone and as co- culture in test tubes containing sterile broth. Bacterial growth and their growth inhibition in co- culture tubes were analyzed till 96 h through spectrophotometric assay and colony forming unit (CFU) assay. Spectrophotometric analysis and CFU/mL assay showed that the effect of B. subtilis on P. aeruginosa and E. coli in co- culture tubes was growth inhibitory. This is accompanied by the reduction in absorbance value and CFU/mL of the co-culture tubes. The bactericidal activity of B. subtilis was measured on P. aeruginosa and E. coli using agar well diffusion method. B. subtilis showed zone of inhibition of 8 mm and 6 mm against P. aeruginosa and E. coli, respectively. The antagonistic activity test of B. subtilis favoured the spectrophotometric and colony forming assay results. These results suggest that B. subtilis develop adaptational pathways by extracellular signaling molecules and antibacterial factors in order to compete with bacterial neighbors.
    Źródło:
    World News of Natural Sciences; 2020, 28; 67-75
    2543-5426
    Pojawia się w:
    World News of Natural Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Ocena zdolności tworzenia biofilmu P. Aeruginosa na próbkach stopu AZ31 uzyskanego w technologii SLM
    Ability of clinical P. Aeruginosa strains to form biofilm on the SLM-produced AZ31 specimens
    Autorzy:
    Pawlak, A.
    Szymczyk, P.
    Chlebus, E.
    Junka, A.
    Secewicz, A.
    Powiązania:
    https://bibliotekanauki.pl/articles/99015.pdf
    Data publikacji:
    2015
    Wydawca:
    Politechnika Śląska. Katedra Biomechatroniki
    Tematy:
    selective laser melting
    AZ31
    P.aeruginosa
    technologie przyrostowe
    Opis:
    W pracy przedstawiono wyniki badań nad oceną zdolności tworzenia biofilmu szczepu bakterii P. Aeruginosa, na powierzchni próbek wyprodukowanych z lekkiego stopu magnezu - AZ31 w technologii SLM. Ilość kolonii bakteryjnych wyhodowanych w biofilnie, jest nawet 700x wyższa, w odniesieniu do skafoldów ze stopu Ti-6Al-7Nb, wyprodukowanych w tej samej technologii.
    In this work, results of research on ability to form biofilm by P. Aeruginosa strains on AZ31 specimens manufactured in SLM technology are reported. The number of cells forming biofilm on specimens is even 700 times higher than on scaffolds manufactured from Ti-6Al-7Nb alloy by SLM technique.
    Źródło:
    Aktualne Problemy Biomechaniki; 2015, 9; 89-92
    1898-763X
    Pojawia się w:
    Aktualne Problemy Biomechaniki
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Novel synthesis of 3-(((E)-benzylidene)amino)-5-((Z)-3,4-dimethoxybenzylidene)-2-phenyl-3,5-dihydro-4H-imidazol-4-one derivatives as potent antimicrobial agents
    Autorzy:
    Manawar, Rohit B.
    Parmar, Mukesh B.
    Nayaka, Indresh J.
    Pandit, Brinda H.
    Shah, Manish K.
    Powiązania:
    https://bibliotekanauki.pl/articles/1066261.pdf
    Data publikacji:
    2019
    Wydawca:
    Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
    Tematy:
    4H-imidazol-4-one
    A. niger
    Anti-fungal
    Anti-microbial Agent
    Bioactivity
    C. albicans
    E. coli
    Elemental analysis
    IR spectroscopy
    Mass spectroscopy
    NMR spectroscopy
    P. aeruginosa
    S. aureus
    S. pyogenes
    Opis:
    Novel synthesis of 4H-imidazol-4-one derivative by reaction of (Z)-3-amino-5-(3,4 dimethoxybenzylidene)-2-phenyl-3,5-dihydro-4H-imidazol-4-one with derivative of different aromatic aldehydes and Salicyldehyde. New synthesized compound are thermally stable and neither air-nor moisture sensitive at RT. Successive library of synthesized compound characterized using IR, NMR, Elemental, Mass, and its antimicrobial activity study.
    Źródło:
    World Scientific News; 2019, 130; 42-70
    2392-2192
    Pojawia się w:
    World Scientific News
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Gastric secretion and the pathogenesis of peptic ulcer in the Helicobacter pylori infection
    Autorzy:
    Konturek, P C
    Konturek, J.W.
    Konturek, S.J.
    Powiązania:
    https://bibliotekanauki.pl/articles/69870.pdf
    Data publikacji:
    1996
    Wydawca:
    Polskie Towarzystwo Fizjologiczne
    Tematy:
    gastric microbiology
    pathogenesis
    infection
    gastric washing
    Campylobacter
    gastric acid
    peptic ulcer
    muscle fibre
    dog
    gastric secretion
    stomach
    leucocyte
    Vibrio rugula
    gastrointestinal tract
    cat
    mice
    epithelial cell
    Pseudomonas aeruginosa
    duodenal ulcer
    gastric mucosa
    gastric pathology
    Helicobacter pylori
    gastric milieu
    mouse
    spiral bacteria
    yeast cell
    Źródło:
    Journal of Physiology and Pharmacology; 1996, 47, 1
    0867-5910
    Pojawia się w:
    Journal of Physiology and Pharmacology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    The chemical digestion of Ti6Al7Nb scaffolds produced by Selective Laser Melting reduces significantly ability of Pseudomonas aeruginosa to form biofilm
    Autorzy:
    Junka, A. F.
    Szymczyk, P.
    Secewicz, A.
    Pawlak, A.
    Smutnicka, D.
    Ziółkowski, G.
    Bartoszewicz, M.
    Chlebus, E.
    Powiązania:
    https://bibliotekanauki.pl/articles/307092.pdf
    Data publikacji:
    2016
    Wydawca:
    Politechnika Wrocławska. Oficyna Wydawnicza Politechniki Wrocławskiej
    Tematy:
    technologia SLM
    Pseudomonas aeruginosa
    skafoldy
    modyfikacja powierzchni
    Ti-6Al-7Nb
    SLM technology
    scaffolds
    surface modification
    Opis:
    In our previous work we reported the impact of hydrofluoric and nitric acid used for chemical polishing of Ti-6Al-7Nb scaffolds on decrease of the number of Staphylococcus aureus biofilm forming cells. Herein, we tested impact of the aforementioned substances on biofilm of Gram-negative microorganism, Pseudomonas aeruginosa, dangerous pathogen responsible for plethora of implant-related infections. The Ti-6Al-7Nb scaffolds were manufactured using Selective Laser Melting method. Scaffolds were subjected to chemical polishing using a mixture of nitric acid and fluoride or left intact (control group). Pseudomonal biofilm was allowed to form on scaffolds for 24 hours and was removed by mechanical vortex shaking. The number of pseudomonal cells was estimated by means of quantitative culture and Scanning Electron Microscopy. The presence of nitric acid and fluoride on scaffold surfaces was assessed by means of IR and rentgen spetorscopy. Quantitative data were analysed using the Mann–Whitney test (P ≤ 0.05). Our results indicate that application of chemical polishing correlates with significant drop of biofilm-forming pseudomonal cells on the manufactured Ti-6Al-7Nb scaffolds ( p = 0.0133, Mann–Whitney test) compared to the number of biofilm-forming cells on non-polished scaffolds. As X-ray photoelectron spectroscopy revealed the presence of fluoride and nitrogen on the surface of scaffold, we speculate that drop of biofilm forming cells may be caused by biofilm-supressing activity of these two elements.
    Źródło:
    Acta of Bioengineering and Biomechanics; 2016, 18, 1; 115-120
    1509-409X
    2450-6303
    Pojawia się w:
    Acta of Bioengineering and Biomechanics
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Impedance Sensors Made in PCB and LTCC Technologies for Monitoring Growth and Degradation of Pseudomonal Biofilm
    Autorzy:
    Chabowski, K.
    Junka, A. F.
    Piasecki, T.
    Nowak, D.
    Nitsch, K.
    Smutnicka, D.
    Bartoszewicz, M.
    Moczała, M.
    Szymczyk, P.
    Powiązania:
    https://bibliotekanauki.pl/articles/221326.pdf
    Data publikacji:
    2017
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    Pseudomonas aeruginosa
    biofilm
    interdigitated sensor
    impedance spectroscopy
    Opis:
    The suitability of low-cost impedance sensors for microbiological purposes and biofilm growth monitoring was evaluated. The sensors with interdigitated electrodes were fabricated in PCB and LTCC technologies. The electrodes were golden (LTCC) or gold-plated (PCB) to provide surface stability. The sensors were used for monitoring growth and degradation of the reference ATCC 15442 Pseudomonas aeruginosa strain biofilm in invitro setting. During the experiment, the impedance spectra of the sensors were measured and analysed using electrical equivalent circuit (EEC) modelling. Additionally, the process of adhesion and growth of bacteria on a sensor’s surface was assessed by means of the optical and SEM microscopy. EEC and SEM microscopic analysis revealed that the gold layer on copper electrodes was not tight, making the PCB sensors susceptible to corrosion while the LTCC sensors had good surface stability. It turned out that the LTCC sensors are suitable for monitoring pseudomonal biofilm and the PCB sensors are good detectors of ongoing stages of biofilm formation.
    Źródło:
    Metrology and Measurement Systems; 2017, 24, 2; 369-380
    0860-8229
    Pojawia się w:
    Metrology and Measurement Systems
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
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