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Tytuł:
Implementation of NSOM to Biological Samples
Autorzy:
Prauzner-Bechcicki, S.
Wiltowska-Zuber, J.
Budkowski, A.
Lekka, M.
Rysz, J.
Powiązania:
https://bibliotekanauki.pl/articles/1490227.pdf
Data publikacji:
2012-02
Wydawca:
Polska Akademia Nauk. Instytut Fizyki PAN
Tematy:
87.64.M-
87.64.mt
Opis:
Near-field scanning optical microscopy is a technique providing images of structures with spatial resolution better than λ/2, which is undetectable in far-field where the Abbe law of limiting resolution is critical. In parallel to the optical imaging, topography maps are also acquired. Near-field scanning optical microscopy measurements can be performed both in air and liquid environments. The later makes the technique very useful for biomaterials analysis offering information that could not be obtained with other methods. Our work presents the results of recent studies on application of near-field scanning optical microscopy to imaging of cells in air as well as in physiological buffers. Differences in cell's topography and morphology have been noticed between two cell lines from human bladder non-malignant (HCV29) and malignant (T24) cancers. Presented results are part of the research that characterizes physiological changes of cells depending on stage of cancer.
Źródło:
Acta Physica Polonica A; 2012, 121, 2; 533-538
0587-4246
1898-794X
Pojawia się w:
Acta Physica Polonica A
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Investigation of Mechanical Properties of Fe-Based Metal Matrix Composites by Warm Compaction for Gear Production
Autorzy:
Gun, T.
Simsir, M.
Powiązania:
https://bibliotekanauki.pl/articles/1032024.pdf
Data publikacji:
2017-03
Wydawca:
Polska Akademia Nauk. Instytut Fizyki PAN
Tematy:
81.20.ev
87.15.La
87.64.M
Opis:
In this study, mechanical behavior of iron-based (Fe-0.8C-2.0Cu-4.5Ni-1.8Mo-1.0B (wt.%)) metal matrix composite synthesized by powder metallurgy was investigated for gear production. Metal matrix composite has been produced by warm compaction, followed by free sintering in controlled Ar gas atmosphere. Green composite was produced under pressure of 650 MPa at 160°C. The green products have been sintered at various temperatures (1050, 1150 and 1250°C) and for various time periods (30, 60 and 90 min). Mechanical tests (hardness and wear tests) have been conducted. The microstructure and the worn surfaces of the samples have been examined under scanning electron microscope and analyzed by energy dispersive spectroscopy and X-ray diffraction method. The results have shown that hardness and wear resistance of the samples increase with increasing sintering temperature and time. Effect of sintering temperature is greater than that of sintering time. The highest hardness and wear resistance have been obtained in the composite sample produced at 1250°C for 90 min.
Źródło:
Acta Physica Polonica A; 2017, 131, 3; 443-447
0587-4246
1898-794X
Pojawia się w:
Acta Physica Polonica A
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The Distribution of Biological Particles Suspended in Normal Cerebrospinal Fluid (CSF)
Autorzy:
Staroń, W.
Herbowski, L.
Powiązania:
https://bibliotekanauki.pl/articles/1537551.pdf
Data publikacji:
2010-07
Wydawca:
Polska Akademia Nauk. Instytut Fizyki PAN
Tematy:
87.64.M-
02.50.-r
61.20.-p
Opis:
Cerebrospinal fluid's functions are protecting, expelling and transporting which are influenced by properties of the fluid. Disorder of one of the functions may bring a disease. Cerebrospinal fluid mainly consists of water (99%), cells and proteins suspended in it. Due to the suspension it can be considered as dispersion medium. Every dispersion medium is characterized by the parameters of particles suspended in it. Parameters of dispersion medium may be classified as single or cumulative parameters. Hence, there is possible to determine additional parameters which characterize CSF in order to give full description of the fluid. The authors present results of research on suspension in normal cerebrospinal fluid worked out on the basis of 2500 microscoping pictures and they also give a statistical analysis of particle diameters. The paper is a part of research project on "Physico-chemical processes in cerebrospinal fluid obtained by puncture from patients diagnosed with the disorders of cerebrospinal fluid (CSF) circulation" realized by Institute of Physics, University of Szczecin in co-operation with Neurosurgery Ward of Public Provincial Hospital Complex in Szczecin.
Źródło:
Acta Physica Polonica A; 2010, 118, 1; 159-160
0587-4246
1898-794X
Pojawia się w:
Acta Physica Polonica A
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Plasmon Enhancement of Fluorescence in Single Light-Harvesting Complexes from em Amphidinium carterae
Autorzy:
Bujak, Ł.
Piątkowski, D.
Mackowski, S.
Wörmke, S.
Jung, C.
Bräuchle, C.
Agarwal, A.
Kotov, N.
Schulte, T.
Hofmann, E.
Brotosudarmo, T.
Scheer, H.
Govorov, A.
Hiller, R.
Powiązania:
https://bibliotekanauki.pl/articles/1807594.pdf
Data publikacji:
2009-12
Wydawca:
Polska Akademia Nauk. Instytut Fizyki PAN
Tematy:
87.80.Nj
87.14.E-
87.64.M-
Opis:
We show that single peridinin-chlorophyll a-protein light-harvesting complexes from dinoflagellate Amphidinium carterae placed near to silver nanoparticles show strongly enhanced fluorescence emission. Single molecule spectroscopy experiments performed at room temperature point toward an enhancement of more than an order of magnitude for optimal conditions. Irrespective of the enhancement, we observe no effect of the metal nanoparticle on the fluorescence emission energy of the complex. This result provides a way to control the optical properties of biomolecules via plasmon excitations in metal nanoparticles.
Źródło:
Acta Physica Polonica A; 2009, 116, S; S-22-S-25
0587-4246
1898-794X
Pojawia się w:
Acta Physica Polonica A
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Preliminary Analysis of Size Distribution of Objects Suspended in Normal Cerebrospinal Fluid in Case of Venticular Hydrocephalic Enlargement and Internal Hydrocephalus
Autorzy:
Staroń, W.
Kubisz, L.
Herbowski, L.
Powiązania:
https://bibliotekanauki.pl/articles/1490409.pdf
Data publikacji:
2012-01
Wydawca:
Polska Akademia Nauk. Instytut Fizyki PAN
Tematy:
87.64.M-
02.50.-r
61.20.-p
Opis:
The paper presents preliminary analysis of normal non-coloured cerebrospinal fluid obtained from patients diagnosed due to suspicion of cerebrospinal fluid malabsorption. According to the findings, the normal cerebrospinal fluid was classified into two groups: A - with clinical diagnosis of ventricular hydrocephalic enlargement and B - with clinical diagnosis of internal hydrocephalus. The analysis of microscopic pictures of normal cerebrospinal fluid in both groups according to numbers and sizes of suspended objects was performed with the aid of Eclipse 600 microscope (with magnification of 1200×) working with a computer by a digital video camera. The authors observed that there is a significant difference in a shape of the distribution curve of objects' diameters between groups A and B. The maximum number of objects in group A was recorded within the range from 5 to 10 μm in diameter size whereas in group B the maximum was recorded within the range from 0.5 to 5 μm in diameter size.
Źródło:
Acta Physica Polonica A; 2012, 121, 1A; A-046-A-048
0587-4246
1898-794X
Pojawia się w:
Acta Physica Polonica A
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Fluorescence Mapping of PCP Light-Harvesting Complexes Coupled to Silver Nanowires
Autorzy:
Krajnik, B.
Piatkowski, D.
Olejnik, M.
Czechowski, N.
Hofmann, E.
Heiss, W.
Mackowski, S.
Powiązania:
https://bibliotekanauki.pl/articles/1419489.pdf
Data publikacji:
2012-08
Wydawca:
Polska Akademia Nauk. Instytut Fizyki PAN
Tematy:
87.64.kv
42.62.Fi
81.07.Pr
87.64.M-
Opis:
In this work we demonstrate confocal fluorescence imaging of hybrid nanostructures composed of silver nanowires and peridinin-chlorophyll-protein light-harvesting complexes. The length of silver nanowires, which reaches 10 μm, allows for determination of the nanowire position and consequently direct correlation with the fluorescence image. In this way we probe the influence of plasmon induced electromagnetic field on the fluorescence of light-harvesting complexes. When the nanowires are spaced from the light-harvesting complexes by a 10 nm thick dielectric layer, we observe a fluorescence enhancement, which depends upon the laser excitation wavelength. The measured enhancement values are 2.5 and 1.9 for 405 nm and 485 nm, respectively. Larger enhancement for the 405 nm excitation is attributed to direct creation of plasmon excitations in the silver nanowires.
Źródło:
Acta Physica Polonica A; 2012, 122, 2; 259-262
0587-4246
1898-794X
Pojawia się w:
Acta Physica Polonica A
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Realization of a Phase Noise Measurement Bench Using Cross Correlation and Double Optical Delay Line
Autorzy:
Salzenstein, P.
Cussey, J.
Jouvenceau, X.
Tavernier, H.
Larger, L.
Rubiola, E.
Sauvage, G.
Powiązania:
https://bibliotekanauki.pl/articles/2047892.pdf
Data publikacji:
2007-11
Wydawca:
Polska Akademia Nauk. Instytut Fizyki PAN
Tematy:
06.30.Ft
43.50.Yw
87.64.M-
87.57.cm
Opis:
In this paper there are presented first results obtained with a phase noise measurement bench operating in X-band, realized in our laboratory, using double optical delay line and cross correlation. Phase noise floor using a microwave sapphire oscillator is better than -160 dB rad$\text{}^{2}$/Hz at 10 kHz from the 10 GHz carrier, using a 2 km optical delay line.
Źródło:
Acta Physica Polonica A; 2007, 112, 5; 1107-1111
0587-4246
1898-794X
Pojawia się w:
Acta Physica Polonica A
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Optical Mini-Disk Resonator Integrated into a Compact Optoelectronic Oscillator
Autorzy:
Salzenstein, P.
Tavernier, H.
Volyanskiy, K.
Kim, N.
Larger, L.
Rubiola, E.
Powiązania:
https://bibliotekanauki.pl/articles/1795637.pdf
Data publikacji:
2009-10
Wydawca:
Polska Akademia Nauk. Instytut Fizyki PAN
Tematy:
06.30.Ft
43.50.Yw
87.64.M-
87.57.cm
Opis:
This work consists in the design, fabrication and characterization of mini-disk $MgF_{2}$ resonators for integration into optoelectronic oscillator and first experimental results of implementation in microwave free spectral range oscillator with taper coupling optoelectronic oscillator.
Źródło:
Acta Physica Polonica A; 2009, 116, 4; 661-663
0587-4246
1898-794X
Pojawia się w:
Acta Physica Polonica A
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Application of μ-FTIR-SR Spectroscopy to Prostate Tissue Analysis
Autorzy:
Kwiatek, W.
Paluszkiewicz, C.
Banaś, A.
Kisiel, A.
Podgórczyk, M.
Marcelli, A.
Cestelli Guidi, M.
Piccinini, M.
Powiązania:
https://bibliotekanauki.pl/articles/1810277.pdf
Data publikacji:
2009-02
Wydawca:
Polska Akademia Nauk. Instytut Fizyki PAN
Tematy:
87.64.km
87.64.kp
87.64.Ee
87.85.jf
Opis:
The infrared spectromicroscopy is a quite recent technique rapidly developing thanks to the availability of new instruments and new brilliant synchrotron radiation sources in different areas and in particular to biomedical researches. In order to achieve a diffraction limited spatial resolution in tissue samples, we performed experiments at SINBAD, the synchrotron infrared beamline of the Laboratori Nazionali di Frascati. We characterized the chemical composition of prostate tissue samples taken from patients affected by prostate cancer disease. Different sizes of the pinholes were considered for the measurements. In the case of prostate tissue sections the results show the possibility to determine the intensity ratio of the $CH_2$ and $CH_3$ bands set at 2930 $cm^{-1}$ and 2960 $cm^{-1}$, respectively. Experiments were also performed with a pinhole of 5 μm of diameter and the differences in both histological and chemical compositions of such samples were determined.
Źródło:
Acta Physica Polonica A; 2009, 115, 2; 602-605
0587-4246
1898-794X
Pojawia się w:
Acta Physica Polonica A
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Surface Study of Selected Biomaterials Using Vibrational Spectroscopy
Autorzy:
Paluszkiewicz, C.
Kwiatek, W.
Długoń, E.
Wesełucha-Birczyńska, A.
Piccinini, M.
Powiązania:
https://bibliotekanauki.pl/articles/1809387.pdf
Data publikacji:
2009-02
Wydawca:
Polska Akademia Nauk. Instytut Fizyki PAN
Tematy:
87.64.km
87.64.kp
87.64.Ee
87.85.jf
Opis:
Vibrational spectroscopy has been extensively used for in vitro and in vivo investigations of degradation mechanism and kinetics of different biomedical materials as well as it has been used to characterize the crystalline and amorphous domains in bio-mineralization process. Infrared and Raman spectroscopy methods are valuable tools in the biomaterials engineering allowing to study processes occurring during their preparation. In vitro tests, where the materials are immersed in simulated body fluids and/or artificial saliva, were used to evaluate the biocompatibility of biomaterials. This kind of tests are a wide range of repeatable and reproducible methods, which are regulated by international standards for commercial use and scientific development of new materials and products. The aim of this work was to examine phase composition of materials applied in dentistry. The bioactivity of such biomaterials was studied by immersing the samples in synthetic body fluid and artificial saliva. The changes were determined by the Fourier transform infrared and Raman microspectroscopy as well as scanning electron microscopy. It was found that results obtained by vibrational spectroscopy show the differences between the studied samples. Chemical reactions occurring during incubation of cements in artificial saliva as well as in synthetic body fluid result in formation of phosphates which deposit on the cement surface.
Źródło:
Acta Physica Polonica A; 2009, 115, 2; 533-536
0587-4246
1898-794X
Pojawia się w:
Acta Physica Polonica A
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Analysis of Human Lenses by Raman Microspectroscopy
Autorzy:
Paluszkiewicz, C.
Chaniecki, P.
Rękas, M.
Rajchel, B.
Piergies, N.
Kwiatek, W.
Powiązania:
https://bibliotekanauki.pl/articles/1398828.pdf
Data publikacji:
2016-02
Wydawca:
Polska Akademia Nauk. Instytut Fizyki PAN
Tematy:
87.64.kp
42.55.Ye
87.64.-t
Opis:
A cataract is an opacity (clouding) of the normally clear lens which develops as a result of aging, metabolic disorders, trauma or heredity. The number of patients with cataract is increasing exponentially. This disease requires surgical intervention, to remove the cloudy lens and to introduce the eye lens polymer. In this work we will present analyses of degraded parts of human lens. Experimental materials were obtained from the lens removed during surgical intervention. These biological samples were measured using Almega XR Confocal Raman spectrometer (Thermo Scientific) with an excitation source of 785 nm laser line. The Raman vibrations in the spectral region of 650-1750 cm¯¹ were analyzed. The difference spectra revealed an excess of tryptophan, tyrosine, phenylalanine, β-sheet conformation, and molecules or molecular groups.
Źródło:
Acta Physica Polonica A; 2016, 129, 2; 244-246
0587-4246
1898-794X
Pojawia się w:
Acta Physica Polonica A
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Determination of the Protein-Binding Properties of Camptothecins by Means of Optical Spectroscopy Methods
Autorzy:
Ziomkowska, B.
Cyrankiewicz, M.
Wybranowski, T.
Kruszewski, S.
Powiązania:
https://bibliotekanauki.pl/articles/1197530.pdf
Data publikacji:
2014-04
Wydawca:
Polska Akademia Nauk. Instytut Fizyki PAN
Tematy:
87.64.K-
87.64.kv
87.15.kp
02.50.Sk
Opis:
Optical spectroscopy methods are widely used in studies of drugs. The affinity of camptothecins - anticancer agents - to human serum albumin (HSA) was determined in this work. Camptothecins (CPTs) exist in two forms: active lactone and open ring inactive carboxylate. In blood, the hydrolysis process of lactone form occurs which leads to deactivation of CPTs. Research is being done on biophysical properties of synthesized CPT compounds, in particular on binding to albumin. The affinity to plasma proteins is an important determinant of stability of CPTs in blood. The following analogues of CPT were tested in this paper: irinotecan, SN-38, topotecan, and 9-amino camptothecin. Using the method of fluorescence anisotropy measurement, the association constants of the studied compounds to HSA were determined. The authors attempted to determine the deactivation rate of topotecan in HSA solution using Principal Component Analysis and Factor Analysis of absorption spectra recorded during hydrolysis process of lactone form.
Źródło:
Acta Physica Polonica A; 2014, 125, 4A; A-61-A-65
0587-4246
1898-794X
Pojawia się w:
Acta Physica Polonica A
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Controlling Structural and Functional Features of Photosynthetic Antenna
Autorzy:
Fiedor, L.
Fiedor, J.
Pilch, M.
Susz, A.
Tworzydło, J.
Michalik, M.
Powiązania:
https://bibliotekanauki.pl/articles/1419488.pdf
Data publikacji:
2012-08
Wydawca:
Polska Akademia Nauk. Instytut Fizyki PAN
Tematy:
87.15.nr
87.15.Cc
87.64.ks
Opis:
In order to gain control over the assembly and functioning of photosynthetic antenna, we have developed methods to manipulate pigment composition of bacterial LH1 complexes via their reconstitution with modified bacteriochlorophylls or carotenoids, major photoactive cofactors of these antennae. In the present work we show how the reconstitution and pigment exchange approach is applied to control structural and functional parameters of LH1 and its subunits. The size of the subunits and the energy of the first excited singlet state can be controlled via the use of detergent while the thermodynamics of LH1 formation can be modified using carotenoids and/or a co-solvent. Carotenoids affect the efficiency of the intracomplex energy transfer, while the replacement of native bacteriochlorophyll a with its Ni-substituted analog allows one to control the excited state properties of LH1. These results show that LH1 is a very promising model system applicable for the design of bio-inspired device performing solar energy conversion.
Źródło:
Acta Physica Polonica A; 2012, 122, 2; 255-258
0587-4246
1898-794X
Pojawia się w:
Acta Physica Polonica A
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
⁵⁷Fe Hyperfine Parameters in Vitamins and Dietary Supplements
Autorzy:
Kądziołka-Gaweł, M.
Michałowska, M.
Powiązania:
https://bibliotekanauki.pl/articles/1388082.pdf
Data publikacji:
2015-02
Wydawca:
Polska Akademia Nauk. Instytut Fizyki PAN
Tematy:
87.64.kx
Opis:
Mössbauer (nuclear gamma-resonance) spectroscopy was used to study various industrial samples of vitamins and dietary supplements containing iron ions, which are used in the anemia treatment. Determination of the iron state (Fe²⁺ or Fe³⁺) in medicaments is important for the pharmaceuticals evaluation quality. The investigated samples contain ferrous fumarate, ferrous gluconiate and ferric diphosphate. ⁵⁷Fe hyperfine parameters of the studied pharmaceuticals indicate that there exist major iron ferrous and ferric compounds. However, Mossbauer spectra of the investigated samples demonstrated the presence of additional ferrous and ferric components, probably related to impurities or to a partially modified main component.
Źródło:
Acta Physica Polonica A; 2015, 127, 2; 653-655
0587-4246
1898-794X
Pojawia się w:
Acta Physica Polonica A
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
ESR Studies of Paramagnetic Centers in Pharmaceutical Materials - Cefaclor and Clarithromycin as an Example
Autorzy:
Skowronska, A.
Wojciechowski, M.
Ramos, P.
Pilawa, B.
Kruk, D.
Powiązania:
https://bibliotekanauki.pl/articles/1490205.pdf
Data publikacji:
2012-02
Wydawca:
Polska Akademia Nauk. Instytut Fizyki PAN
Tematy:
87.15.La
87.64.kj
87.64.kh
75.10.Dg
Opis:
Electron spin resonance (ESR) studies of two antibiotics, Cefaclor and Clarithromycin, have been performed in order to investigate concentration and dynamics of free radicals generated in these compounds due to thermal sterilization. For Cefaclor three combinations of temperature and heating time have been applied: 160°C, 170°C, 180°C for 120 min, 60 min, and 30 min, respectively, according to the pharmaceutical sterilization norm. Clarithromycin has been heated at 160°C for 120 min. The ESR lineshape has been investigated versus microwave power ranging from 2.2 mW to 70 mW. Electron spin-spin relaxation time has been estimated from the ESR lineshape analysis. Concentrations of radicals generated due to different sterilization procedures have been compared with the purpose to select the best sterilization scheme.
Źródło:
Acta Physica Polonica A; 2012, 121, 2; 514-517
0587-4246
1898-794X
Pojawia się w:
Acta Physica Polonica A
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Interactions of Photosystem I with Plasmonic nanostructures
Autorzy:
Hussels, M.
Nieder, J.
Powiązania:
https://bibliotekanauki.pl/articles/1419504.pdf
Data publikacji:
2012-08
Wydawca:
Polska Akademia Nauk. Instytut Fizyki PAN
Tematy:
73.20.Mf
87.64.kv
87.80.Nj
Opis:
The plasmonic interaction effects of various nanostructures on the fluorescence properties of photosystem I as found by single-molecule spectroscopy are summarized. The used nanostructures are spherical Au nanoparticles, silver island films as well as hexagonal arrays of nanometer-sized Au- and Ag-triangles (the Fischer patterns). The fluorescence emission of photosystem I is intensified due to coupling with these nanostructures. For single photosystem I complexes, enhancement factors of up to 37 were observed. The average enhancements vary between 2.2 for Au Fischer pattern and 9 for spherical Au nanoparticles. The enhancement of the fluorescence of photosystem I demonstrates in all cases a strong wavelength dependence. This wavelength dependence can be explained by the spatially largely extended multichromophore composition of photosystem I complexes. From the viewpoint of the usability of these nanostructures for spectroscopic signal enhancement, the Fischer patterns are beneficial, due to their very low autoluminescence.
Źródło:
Acta Physica Polonica A; 2012, 122, 2; 269-274
0587-4246
1898-794X
Pojawia się w:
Acta Physica Polonica A
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Chemical Composition of Atherosclerotic Plaques οf apoE/LDLR-Double Knockout Mice by Synchrotron Radiation FTIR Microspectroscopy
Autorzy:
Kowalska, J.
Gajda, M.
Kwiatek, W.
Franczyk-Żarów, M.
Kostogrys, R.
Chłopicki, S.
Sandt, C.
Dumas, P.
Powiązania:
https://bibliotekanauki.pl/articles/1490259.pdf
Data publikacji:
2012-02
Wydawca:
Polska Akademia Nauk. Instytut Fizyki PAN
Tematy:
87.19.xr
87.64.km
87.64.-t
Opis:
Atherosclerosis is a multietiological inflammatory disease of large and medium-sized arteries of increasing incidence in westernized countries. The aim of this study was to identify the biochemical changes during the progression of atherosclerosis by synchrotron radiation Fourier transform infrared microspectroscopy in atheromas of apoE/$LDLR^{-//-}$ mice fed egg-rich diet supplemented or not with angiotensin converting enzyme inhibitor perindopril. Synchrotron radiation Fourier transform infrared microspectroscopy technique was used to obtain information at high spatial resolution about the distribution of proteins (C-N, N-H, CO for amide I and amide II bands), lipids ($CH_2$, $CH_3$ bands) as well as mineral deposits (calcium carbonates and phosphates). Total contents of lipids and proteins were found to be significantly lower in animals treated with the diet and perindopril. An increase in saturation level of lipids was observed in animals fed with egg-rich diet when compared to the normal diet and perindopril treatment, which did not inhibit this effect. Moreover, a significant change in the secondary structure of proteins (ratio between absorption bands 1634 $cm^{-1}$/1656 $cm^{-1}$ attributed to β-type and α-type, respectively) was observed in both experimental groups in comparison with the control. Principal component analysis was used to analyse the recorded spectra. It has revealed that higher content of phosphates (wavenumber range 950-1020 $cm^{-1}$) was observed between egg-rich diet fed animals and the control group.
Źródło:
Acta Physica Polonica A; 2012, 121, 2; 555-560
0587-4246
1898-794X
Pojawia się w:
Acta Physica Polonica A
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Structural Changes of DPPC Bilayers Induced by Gemini Surfactant
Autorzy:
Kida, W.
Kozak, M.
Powiązania:
https://bibliotekanauki.pl/articles/1431597.pdf
Data publikacji:
2012-04
Wydawca:
Polska Akademia Nauk. Instytut Fizyki PAN
Tematy:
87.15.Zg
61.05.cf
87.64.km
87.16.dt
Opis:
The phospholipid-gemini surfactant systems are promising agents for construction of lipoplexes for DNA delivery systems in gene therapy. In this work the influence of a gemini surfactant - 1,1'-(1,6-hexan)bis3-octyloxymethylimidazolium di-chloride) (IMI_Cl_C6_C8) on the structure and phase behaviour of aqueous suspensions of the fully hydrated phospholipid - 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) were characterised using small angle scattering of synchrotron radiation (SAXS), differential scanning calorimetry (DSC) and Fourier transform infrared spectroscopy (FTIR). The surfactant studied caused a destabilisation of the lamellar phase typical of DPPC. Addition of the surfactant also shifted to lower temperatures and extended the range of the main phase transition
Źródło:
Acta Physica Polonica A; 2012, 121, 4; 893-898
0587-4246
1898-794X
Pojawia się w:
Acta Physica Polonica A
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Micro-Raman Spectroscopy of Natural and Synthetic Ferritins and Their Mimetics
Autorzy:
Szybowicz, M.
Koralewski, M.
Karoń, J.
Melnikova, L.
Powiązania:
https://bibliotekanauki.pl/articles/1386994.pdf
Data publikacji:
2015-02
Wydawca:
Polska Akademia Nauk. Instytut Fizyki PAN
Tematy:
87.15.-v
87.64.Je
Opis:
Micro-Raman spectroscopy was used to studies of naturally occurring biogenic ferritin, synthetic ferritin with magnetic core (magnetoferritin) as well as their several mimetics. We demonstrate the ability of micro-Raman spectroscopy to discriminate between ferritin and magnetoferritin used in the studies. The results are promising for further use of the Raman spectroscopy as potential tool to distinguish between the forms of iron present in biogenic materials and biological tissues.
Źródło:
Acta Physica Polonica A; 2015, 127, 2; 534-536
0587-4246
1898-794X
Pojawia się w:
Acta Physica Polonica A
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Mössbauer Spectrometry Study of Biological Tissues
Autorzy:
Bonková, I.
Miglierini, M.
Bujdoš, M.
Kopáni, M.
Powiązania:
https://bibliotekanauki.pl/articles/1032139.pdf
Data publikacji:
2017-04
Wydawca:
Polska Akademia Nauk. Instytut Fizyki PAN
Tematy:
33.45.+x
87.64.kx
31.30.Gs
87.15.B-
75.75.-c
Opis:
Magnetic microstructure of iron contained in selected biological tissues is characterized and mutually compared. We have studied three types of biological samples prepared from human brain, human and horse spleen. Original samples were lyophilized (dried in a vacuum) thus providing powder forms. As a principal method of study, ⁵⁷Fe Mössbauer spectrometry in transmission mode was used. The Mössbauer spectrometry experiments were performed at room ( ≈300 K) and at liquid helium (4.2 K) temperature. At room temperature Mössbauer spectra show doublet-like features. Such behaviour indicates possible presence of nanoparticles with fluctuating magnetic moments that acquire arbitrary positions. On the other hand, low temperature Mössbauer spectrometry measurements demonstrate significant contribution of sextets that confirmed occurrence of blocked magnetic moments of iron-containing particles. Different relative contributions of magnetic components in the low temperature spectra for the three inspected biological tissues suggest differences in the blocking temperatures of the magnetic nanoparticles present in them.
Źródło:
Acta Physica Polonica A; 2017, 131, 4; 1081-1083
0587-4246
1898-794X
Pojawia się w:
Acta Physica Polonica A
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Gold Nanoparticles with Elongated Shapes: Synthesis and Optical Properties
Autorzy:
Olejnik, M.
Agarwal, A.
Zaleszczyk, W.
Kotov, N.
Mackowski, S.
Powiązania:
https://bibliotekanauki.pl/articles/1419540.pdf
Data publikacji:
2012-08
Wydawca:
Polska Akademia Nauk. Instytut Fizyki PAN
Tematy:
81.07.-b
81.07.Pr
87.64.kv
87.64.K-
Opis:
We use a seed-mediated growth to fabricate gold nanorods in water solution. By changing the amount of silver salt we demonstrate the control of aspect ratio of the obtained rods. The microscopic and spectroscopic analyses provide ways to characterize the morphology and the optical properties of the gold nanorods. Nanorods synthesized using this approach feature length of the order of 50-60 nm, which translates into longitudinal plasmon resonances in the near infrared spectrum region.
Źródło:
Acta Physica Polonica A; 2012, 122, 2; 346-349
0587-4246
1898-794X
Pojawia się w:
Acta Physica Polonica A
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Challenges in Biology and Medicine with Synchrotron Infrared Light
Autorzy:
Dumas, P.
Miller, L.
Tobin, M.
Powiązania:
https://bibliotekanauki.pl/articles/1808306.pdf
Data publikacji:
2009-02
Wydawca:
Polska Akademia Nauk. Instytut Fizyki PAN
Tematy:
87.80.Dj
87.80.-y
87.64.km
87.56.B-
87.19.xj
87.16.-b
87.15.bd
78.30.-j
Opis:
The brightness (or brilliance) of synchrotron radiation was exploited in infrared microspectrosocopy. Among application of this synchrotron-based microanalytical technique, biological and biomedical investigations, at the diffraction-limited spot size, are exhibit of an increasing interest among almost all the existing infrared beamline worldwide. This paper is presenting the main properties of such a source, coupled with an infrared microscope. Several important applications in biomedical field are reported: cancer cells studies and drug effects, human substantia nigra in Parkinson's disease, β-amyloids deposits in Alzheimer's disease.
Źródło:
Acta Physica Polonica A; 2009, 115, 2; 446-454
0587-4246
1898-794X
Pojawia się w:
Acta Physica Polonica A
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
NMR Relaxation and ESR Lineshape of Anisotropically Rotating Paramagnetic Molecules
Autorzy:
Kubica, A.
Milewska, A.
Noinska, M.
Earle, K.
Kruk, D.
Powiązania:
https://bibliotekanauki.pl/articles/1490224.pdf
Data publikacji:
2012-02
Wydawca:
Polska Akademia Nauk. Instytut Fizyki PAN
Tematy:
87.64.kj
87.64.kh
75.10.Dg
76.60.Es
Opis:
The role of anisotropic rotation for electron spin resonance lineshape and nuclear magnetic relaxation dispersion profiles for paramagnetic molecules with electron spin quantum number S ≥ 1 is discussed. The ESR spectra and nuclear magnetic relaxation dispersion profiles are calculated by means of an approach based on the stochastic Liouville equation and referred to in the literature as "Swedish slow motion theory". This description is valid for arbitrary motional conditions and interaction strengths. Molecular tumbling influences the ESR spectra by modulating zero field splitting interactions. The nuclear spin relaxation is affected by the rotational motion in a twofold way: via the electron spin dynamics and as a direct source of modulations of the electron-nuclear dipole-dipole interactions. For coinciding principal axes systems of the permanent (residual, static) zero field splitting and rotational tensors the ESR lineshape is not affected by rotational anisotropy. Rotational anisotropy is important for nuclear relaxation as it is influenced by molecular rotation not only via the electron spin dynamics, but also directly by modulations of the electron spin-nuclear spin dipole-dipole interaction (when the dipole-dipole and zero field splitting frames do not coincide). The anisotropy effects depend strongly on the relative orientation of the dipole-dipole and permanent zero field splitting axes. Nevertheless, a different scenario is also possible. When the diffusion axis coincides with the dipole-dipole axis (but not with the principal axis system of the permanent zero field splitting), the nuclear spin relaxation as well as the ESR lineshape, become sensitive to the rotational anisotropy. The possible dependence of the ESR lineshape and nuclear spin relaxation on the rotational anisotropy should be carefully considered when attempting a joint analysis of ESR and nuclear magnetic relaxation dispersion results for paramagnetic molecules.
Źródło:
Acta Physica Polonica A; 2012, 121, 2; 527-532
0587-4246
1898-794X
Pojawia się w:
Acta Physica Polonica A
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Status and Vision for Structural Biology at the Canadian Light Source
Autorzy:
Grochulski, P.
Fodje, M.
George, G.
Powiązania:
https://bibliotekanauki.pl/articles/1431525.pdf
Data publikacji:
2012-04
Wydawca:
Polska Akademia Nauk. Instytut Fizyki PAN
Tematy:
87.64.kd
87.80.Dj
Opis:
The status and vision for Structural Biology at the Canadian Light Source (CLS) is presented. The beamlines that have been described in the paper represent a Canadian national resource that is available to science and industry world-wide. They include state-of-the-art infrastructure and include specialized capabilities, many of which are not available elsewhere, including macromolecular crystallography, biological X-ray spectroscopy, soft X-ray spectromicroscopy, as well as small angle and wide angle X-ray scattering beamlines. The vision for Structural Biology at the Canadian Light Source is significantly enhanced by the synergies and collaborations between the users of the different beamlines and by the strengths of the scientific personnel and trainees.
Źródło:
Acta Physica Polonica A; 2012, 121, 4; 866-870
0587-4246
1898-794X
Pojawia się w:
Acta Physica Polonica A
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Sulphur XANES Analysis of Cultured Human Prostate Cancer Cells
Autorzy:
Kwiatek, W.
Podgórczyk, M.
Paluszkiewicz, Cz.
Balerna, A.
Kisiel, A.
Powiązania:
https://bibliotekanauki.pl/articles/1812260.pdf
Data publikacji:
2008-08
Wydawca:
Polska Akademia Nauk. Instytut Fizyki PAN
Tematy:
87.64.kd
87.19.xj
Opis:
Prostate cancer is one of the most commonly diagnosed cancers in men throughout the world. It is believed that changes to the structure of protein binding sites, altering its metabolism, may play an important role in carcinogenesis. Sulphur, often present in binding sites, can influence such changes through its chemical speciation. Hence there is a need for precise investigation of coordination environment of sulphur. X-ray absorption near edge structure spectroscopy offers such possibility. Cell culture samples offer histologically well defined areas of good homogeneity, suitable for successful and reliable X-ray absorption near edge structure analysis. This paper presents sulphur speciation data collected from three different human prostate cancer cell lines (PC-3, LNCaP and DU-145). Sulphur X-ray absorption near edge structure analysis was performed on K-edge structure. The spectra of cells were compared with those of cancerous tissue and with organic substances as well as inorganic compounds.
Źródło:
Acta Physica Polonica A; 2008, 114, 2; 463-470
0587-4246
1898-794X
Pojawia się w:
Acta Physica Polonica A
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Assessing the Indoor Pollutants Effect on Ornamental Plants Leaves by FT-IR Spectroscopy
Autorzy:
Husti, A.
Cantor, M.
Stefan, R.
Miclean, M.
Roman, M.
Neacsu, I.
Contiu, I.
Magyari, K.
Baia, M.
Powiązania:
https://bibliotekanauki.pl/articles/1398690.pdf
Data publikacji:
2016-01
Wydawca:
Polska Akademia Nauk. Instytut Fizyki PAN
Tematy:
87.14.-g
87.15.B-
87.64.km
92.60.Sz
Opis:
Air pollution has become a mass phenomenon, a major and global problem of modern society, affecting billions of people and environment. People are exposed to various levels of pollutants not just in the outdoor environment, but also in indoors. The quality of life and well-being of employees can be increased by incorporating ornamental plants in the work environment. Among the great variety of plants species able to remove/reduce indoor air pollutants Dracaena deremensis, Sansevieria trifasciata and Ficus elastica were hereby investigated. Their ability to remove chemical pollutants was evaluated in real-life conditions and the changes induced by the environmental stress on the structure and biochemical composition of the plants leaves were evidenced by the Fourier transform infrared spectroscopy. The most pronounced concentration decrease was noticed for the CO₂ (58.33% removed concentration), whereas the mean value of the removed concentration of other chemical pollutants was of ≈ 25%. The Fourier transform infrared spectra analysis revealed that, although the plants are subjected to the chemical pollutants action, they maintain the structure by adjusting their metabolism. A decrease in the overall protein contribution in the amide bands and an increase of the bands assigned to polysaccharide vibrations, illustrate the consequences of the pollution action. Moreover, the chlorophyll presence is evidenced in the IR spectra of all samples by the bands around 1040, 1445, 1620, and 1735 cm^{-1}. The results show that the Fourier transform infrared spectra are an important source of information for the rapid characterization of the chemical structure of the biological systems under environmental stress.
Źródło:
Acta Physica Polonica A; 2016, 129, 1; 142-149
0587-4246
1898-794X
Pojawia się w:
Acta Physica Polonica A
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Stability of ferrous fumarate in medicaments for women: Application of Mössbauer spectroscopy
Autorzy:
Kądziołka-Gaweł, M.
Wojtyniak, M.
Powiązania:
https://bibliotekanauki.pl/articles/1065006.pdf
Data publikacji:
2016-06
Wydawca:
Polska Akademia Nauk. Instytut Fizyki PAN
Tematy:
87.64.kx
Opis:
Determination of the iron state (Fe²⁺ or Fe³⁺) and content of iron in commercial pharmaceutical products containing ferrous fumarate FeC₄H₂O₄ was made by the Mössbauer spectroscopy and X-ray fluorescence method. Also, influence of thermal treatment on stability of ferrous fumarate in selected medicaments has been investigated. The investigated samples were annealed in definite temperature: 373, 473, and 573 K for 5 h. Room temperature Mössbauer spectra of initial samples gave clear evidence that two phases of iron were presented. The major component was connected with ferrous fumarate with a contribution from ≈85% to ≈50%, depending on investigated medicaments. Ferrous fumarate was stable up to annealing temperature 473 K. Above this temperature significant oxidation of Fe²⁺ to Fe³⁺ was observed.
Źródło:
Acta Physica Polonica A; 2016, 129, 6; 1242-1244
0587-4246
1898-794X
Pojawia się w:
Acta Physica Polonica A
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Methodology for FTIR Imaging of Individual Cells
Autorzy:
Yao, Seydou
Cestelli Guidi, M.
Delugin, M.
Della-Ventura, G.
Marcelli, A.
Petibois, C.
Powiązania:
https://bibliotekanauki.pl/articles/1398831.pdf
Data publikacji:
2016-02
Wydawca:
Polska Akademia Nauk. Instytut Fizyki PAN
Tematy:
82.80.-d
87.85.Pq
87.64.-t
Opis:
FTIR imaging is a novel spectroscopic technique able to provide cell imaging, in vivo and in real-time. However, one key issue is developing methodologies for cell culture on IR-transparent substrates fitting cell biology requirements. In this work we tested different IR-transparent substrates in terms of biotoxicity, surface properties, and spectral image acquisition qualities. Only a few substrates, namely Si₃N₄, Ge, GLS, LaF₃, Si, SrF₂, ZnS/C, ZnS/F, were found to provide cell culture conditions comparable to those observed on usual polycarbonate Petri dishes, the main limiting parameter being the toxicity of the material (ZnS, GLS, PbF₂, PbCl₂) or a poor adhesiveness (notably diamond, AgCl, CaF₂, ZnS). From substrates eligible for a good-quality cell culture, the spectral acquisition quality is mainly affected by the refractive index value. Finally, the best compromise between cell culture quality and image spectral quality could be obtained using Si and Ge substrates. This rationalization of the available IR-transparent substrates for bioimaging is particularly relevant for live cell analyses, where cell culture conditions must remain unaffected by substrate properties.
Źródło:
Acta Physica Polonica A; 2016, 129, 2; 250-254
0587-4246
1898-794X
Pojawia się w:
Acta Physica Polonica A
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Optical Properties of Acupunctural Points as Diagnostic Method
Autorzy:
Jovanić, B.
Nikolovski, D.
Radenković, B.
Despotović, M.
Powiązania:
https://bibliotekanauki.pl/articles/1795707.pdf
Data publikacji:
2009-10
Wydawca:
Polska Akademia Nauk. Instytut Fizyki PAN
Tematy:
87.64.-t
Opis:
The goal of this work was to establish a connection between the optical parameters of the skin in which the acupuncture points have been settled and the pathological state of the organism. For the first time a connection between the optical characteristics of the specific skin parts (acupuncture points) and the state of the organism has been demonstrated. The optical parameter of the acupuncture point optical parameter (on surface of the reflectance curve between 470 nm and 660 nm) conduct in accordance with the traditional Chinese medicine theories (balance disturbance within the organism, time change). Acquired results point to the possibility of using acupuncture point optical parameter measuring as a method to determine the number of therapeutical treatments during the traditional Chinese medical treatments.
Źródło:
Acta Physica Polonica A; 2009, 116, 4; 693-696
0587-4246
1898-794X
Pojawia się w:
Acta Physica Polonica A
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Computational Method of X-Ray Harmonic Elimination in EXAFS Treating
Autorzy:
Ovsjannikov, Ph. M.
Powiązania:
https://bibliotekanauki.pl/articles/1931720.pdf
Data publikacji:
1994-11
Wydawca:
Polska Akademia Nauk. Instytut Fizyki PAN
Tematy:
87.64.Fb
78.70.Dm
Opis:
The computational method of EXAFS amplitude correction by harmonics elimination and intensity leakage assessment has been worked out. In conventional absorption measurements, the major sources of errors are the transition of harmonics by the monochromator and inevitable leakage of radiation around and (or) through the sample. In this paper we present the method of the effective corrections being based on the measurements of the intensity of X-ray radiation passed through calibrated metal foils both without and together with investigated samples. As the advantage of the method one may emphasise the fact that it does not require any additional data for the investigated samples such as chemical composition, density, table absorption coefficients.
Źródło:
Acta Physica Polonica A; 1994, 86, 5; 749-752
0587-4246
1898-794X
Pojawia się w:
Acta Physica Polonica A
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Primary Electron Transfer Reactions in Membrane-Bound Open and Closed Reaction Centers from Purple Bacterium Rhodobacter sphaeroides
Autorzy:
Gibasiewicz, K.
Pajzderska, M.
Karolczak, J.
Burdziński, G.
Dobek, A.
Jones, M.
Powiązania:
https://bibliotekanauki.pl/articles/1419491.pdf
Data publikacji:
2012-08
Wydawca:
Polska Akademia Nauk. Instytut Fizyki PAN
Tematy:
82.50.-m
87.64.K-
87.15.M-
Opis:
Most ultrafast transient absorption studies of primary electron transfer in reaction centers from purple bacteria have been performed in complexes isolated from their natural lipid membrane environment using detergent. In this contribution we present near-UV-vis transient absorption studies of reaction centers embedded in their natural membrane environment. The evolution of absorption spectra recorded with subpicosecond resolution and reflecting primarily electron transfer reactions has been compared to data obtained previously for isolated reaction centers. We conclude that the overall spectral evolution in both types of samples is similar, and the environment of the reaction center protein has only a minor effect on the primary electron transfer reactions. The differences between the two samples are explained in terms of different energetic levels (and their different temporal evolution) of the two initial charge separated states $P^+B_A^-$ and $P^+H_A^-$, with P being the primary electron donor and $B_A$ and $H_A$ the two consecutive electron acceptors. Additionally, in the electric field generated by $P^+H_A^-,$ $B_A$ in membrane-bound reaction centers undergoes a stronger electrochromic shift than in isolated reaction centers.
Źródło:
Acta Physica Polonica A; 2012, 122, 2; 263-268
0587-4246
1898-794X
Pojawia się w:
Acta Physica Polonica A
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Molecular Interaction between Bacterial Antigens and Macrophage Receptors Studied by Atomic Force Microscopy
Autorzy:
Targosz, M.
Czuba, P.
Biedroń, R.
Strus, M.
Gamian, A.
Marcinkiewicz, J.
Szymoński, M.
Powiązania:
https://bibliotekanauki.pl/articles/2044777.pdf
Data publikacji:
2006-03
Wydawca:
Polska Akademia Nauk. Instytut Fizyki PAN
Tematy:
82.37.Rs
87.64.Dz
87.15.Kg
Opis:
Atomic force spectroscopy was used to study interaction strengths between bacterial antigens and receptors on macrophages. This method allowed for a direct comparison of the interaction strengths in different systems studied at the level of single molecules.
Źródło:
Acta Physica Polonica A; 2006, 109, 3; 421-426
0587-4246
1898-794X
Pojawia się w:
Acta Physica Polonica A
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Distinguishing Prostate Cancer from Hyperplasia
Autorzy:
Kwiatek, W. M.
Banaś, A.
Banaś, K.
Podgórczyk, M.
Dyduch, G.
Falkenberg, G.
Gajda, M.
Cichocki, T.
Powiązania:
https://bibliotekanauki.pl/articles/2044731.pdf
Data publikacji:
2006-03
Wydawca:
Polska Akademia Nauk. Instytut Fizyki PAN
Tematy:
87.64.-t
Opis:
The prostate gland is the most common site of neoplastic disorders in men. The knowledge of the pathogenesis of benign prostate hyperplasia and prostate cancer is still under investigation. X-ray fluorescence analysis using synchrotron radiation seems to be an appropriate technique to study the elemental composition of biological tissues. The measurements presented in this paper were carried out on the L-beam line at the HASYLAB, DESY (Germany). The observed concentrations of Fe, Cu, Mn, and Zn are significantly higher in cancerous tissues, as compared to normal and hyperplastic ones. Our results reveal also the existence of the two types of hyperplasia.
Źródło:
Acta Physica Polonica A; 2006, 109, 3; 377-381
0587-4246
1898-794X
Pojawia się w:
Acta Physica Polonica A
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Laser- and Light-Induced Autofluorescence Spectroscopy of Human Skin in Dependence on Excitation Wavelengths
Autorzy:
Bliznakova, I.
Borisova, E.
Avramov, L.
Powiązania:
https://bibliotekanauki.pl/articles/2047898.pdf
Data publikacji:
2007-11
Wydawca:
Polska Akademia Nauk. Instytut Fizyki PAN
Tematy:
42.72.Bj
87.15.M-
87.64.kv
Opis:
Human skin contains various types of native fluorophores and absorbers with unique absorption and emission spectra, different quantum efficiency, concentration and spatial distribution within the skin. Autofluorescence spectroscopy is applied as diagnostic tool for cutaneous tumor detection that increases the importance of evaluation of natural existing fluorophores and unification of data for given class of pathologies. In the current study, several excitation sources in the region 337-405 nm are applied, to achieve information about typical autofluorescent properties of normal human skin.
Źródło:
Acta Physica Polonica A; 2007, 112, 5; 1131-1136
0587-4246
1898-794X
Pojawia się w:
Acta Physica Polonica A
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Magneto-Optical Study Toward Discrimination of Iron Mineral in Human Tissues
Autorzy:
Koralewski, M.
Żurawski, J.
Langkammer, C.
Karoń, J.
Haybaeck, J.
Powiązania:
https://bibliotekanauki.pl/articles/1030495.pdf
Data publikacji:
2018-03
Wydawca:
Polska Akademia Nauk. Instytut Fizyki PAN
Tematy:
87.15.-v
87.64.Ni
78.20.Ls
Opis:
Magnetic optical rotatory dispersion (MORD) of thin selected biological tissues and thin film of composite made from akaganeite mineral and PVA as well as ferritin and their mimetics aqueous suspensions were performed in spectral range 250-650 nm at room temperature. Good correlation between MORD spectra for akaganeite composite film, ferritin and their mimetics aqueous suspensions with spectra of thin slices of human tissue obtained from white matter of the brain and spleen were observed. Comparison suggest a contribution from Fe(III) to MORD spectra of tissues. This preliminary results show that application of MORD spectroscopy to clinical analysis may be useful.
Źródło:
Acta Physica Polonica A; 2018, 133, 3; 742-744
0587-4246
1898-794X
Pojawia się w:
Acta Physica Polonica A
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Hydrothermal Synthesis of Hydroxyapatite on Titanium after Anodic Oxidation
Autorzy:
Strzała, A.
Simka, W.
Marszałek, M.
Powiązania:
https://bibliotekanauki.pl/articles/1490260.pdf
Data publikacji:
2012-02
Wydawca:
Polska Akademia Nauk. Instytut Fizyki PAN
Tematy:
85.85.+j
81.20.Ka
82.80.Gk
87.64.Bx
87.85.J-
Opis:
Hydrothermal synthesis of hydroxyapatite (HA) is a method which is relatively easy to apply and enables HA precipitation on substrates of various shapes, which is vital to endoprostheses fabrication. Anodic oxidation facilitates HA precipitation, making the coating thicker and more uniform. In this paper the influence of anodic oxidation of titanium substrates on HA precipitation in hydrothermal synthesis is discussed. To determine chemical composition and coating uniformity of anodised and polished Ti substrates the Raman microspectroscopy was employed. The composition was also confirmed using X-ray diffraction method. HA coatings on Ti after anodic oxidation exhibit higher uniformity in comparison to untreated Ti. The X-ray diffraction patterns showed that the HA coating was partly amorphous. Also influence of additional treatment (soaking in NaOH and/or HBSS) after anodic oxidation is discussed in the present paper. It seems that pretreatment may be favourable in some cases, but if the anodic oxidation was conducted in the presence of calcium phosphates the pretreatment seems to prevent the HA precipitation.
Źródło:
Acta Physica Polonica A; 2012, 121, 2; 561-564
0587-4246
1898-794X
Pojawia się w:
Acta Physica Polonica A
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
X-ray Diffraction and Elemental Analysis of Medical and Environmental Samples
Autorzy:
Bielecka, K.
Kurtek, W.
Banaś, D.
Kubala-Kukuś, A.
Braziewicz, J.
Majewska, U.
Pajek, M.
Wudarczyk-Moćko, J.
Stabrawa, I.
Powiązania:
https://bibliotekanauki.pl/articles/1197663.pdf
Data publikacji:
2014-04
Wydawca:
Polska Akademia Nauk. Instytut Fizyki PAN
Tematy:
78.70.En
87.64.Bx
32.30.Rj
87.64.kv
Opis:
The results of the elemental and chemical composition analysis of human medical samples (blood, serum, hair, urine, tooth, kidney stones, gallstones) and environmental samples (slag, cereal, vegetables, flour, pork bones, pork meat, fish) are presented. The analysis were performed by application of the total reflection X-ray fluorescence, wavelength dispersive X-ray fluorescence and X-ray powder diffraction methods. With X-ray fluorescence methods the following elements were identified: O, Na, Mg, Al, Si, P, S, Cl, K, Ca, Ti, Mn, Fe, Ni, Cu, Zn, Se, Br, Rb, Sr, Zr, I, Ba, and Pb, whose concentrations were from a few ng/g to tens of percent. For some samples the elemental analysis was extended by X-ray powder diffraction measurements. With this method the chemical composition was determined. In the paper the experimental setups, methodology of samples preparation and methods of carrying out the measurements are described. As an example the X-ray spectra registered for gallstone sample are discussed in detail. Finally, the results of X-ray diffraction and elemental analysis for selected medical and environmental samples are summarized.
Źródło:
Acta Physica Polonica A; 2014, 125, 4; 911-918
0587-4246
1898-794X
Pojawia się w:
Acta Physica Polonica A
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
AFM Investigation of Biological Nanostructures
Autorzy:
Strzelecki, J.
Dąbrowski, M.
Strzelecka, J.
Tszydel, M.
Mikulska, K.
Nowak, W.
Balter, A.
Powiązania:
https://bibliotekanauki.pl/articles/1419535.pdf
Data publikacji:
2012-08
Wydawca:
Polska Akademia Nauk. Instytut Fizyki PAN
Tematy:
87.64.Dz
81.07.-b
Opis:
Nanostructures created by living organisms, optimized through millions of years of evolution, can be a valuable inspiration for nanotechnology. We employ atomic force microscopy to examine such structures in materials created by common organisms - caddisfly and diatoms. Caddisfly larvae are well known for their ability to spin silk, which serves as an "adhesive tape" to glue various materials and collect food in aqueous environment. Atomic force microscopy imaging of caddisfly silk, performed for the first time by our team, has shown that its surface is patterned with 150 nm extensions - a feature related to its exceptional underwater sticking abilities. Results of force spectroscopy of protein structures found on the surface are also shown. A characteristic feature of diatoms is that they are encased within a unique silica cell wall called frustules, patterned with 200 nm pores, which allow cellular interaction with the environment. We perform atomic force microscopy imaging of frustules in living diatoms as well as adhesion measurements inside pores.
Źródło:
Acta Physica Polonica A; 2012, 122, 2; 329-332
0587-4246
1898-794X
Pojawia się w:
Acta Physica Polonica A
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Spectroscopy of Photosynthetic Pigment-Protein Complex LHCII
Autorzy:
Gruszecki, W.
Zubik, M.
Luchowski, R.
Grudzinski, W.
Gryczynski, Z.
Gryczynski, I.
Powiązania:
https://bibliotekanauki.pl/articles/1490052.pdf
Data publikacji:
2012-02
Wydawca:
Polska Akademia Nauk. Instytut Fizyki PAN
Tematy:
87.64.-t
Opis:
Light-harvesting pigment-protein complex of photosystem II is the most abundant membrane protein in the biosphere, comprising more than half chlorophyll molecules. The protein plays a role of photosynthetic antenna, collecting solar radiation and transferring excitations towards the reaction centers, where electric charge separation takes place. Efficient excitation energy capture and transfer requires unique organization of the complex and unique photophysical properties of the accessory pigments: chlorophylls and carotenoids. LHCII is also a place where extremely harmful singlet oxygen may be generated, under strong illumination conditions. Several physical mechanisms have been found in LHCII, operating to protect the photosynthetic apparatus against light-induced damage, including chlorophyll triplet and singlet excitations quenching by carotenoids. In this paper we discuss the results of our recent studies, carried out with the application of several molecular spectroscopy techniques (electronic absorption, fluorescence, resonance Raman and FTIR), designed to investigate molecular mechanisms responsible for regulation of excitation density in LHCII. Among the most interesting findings are the light-induced molecular configuration changes of the LHCII-bound xanthophylls, leading to conformational rearrangements of the protein. These mechanisms are discussed in terms of excessive excitation quenching in the pigment-protein complex subjected to overexcitation. Such an activity seems to represent a vital regulatory process in the photosynthetic apparatus, at the molecular level, protecting plants against photodegradation.
Źródło:
Acta Physica Polonica A; 2012, 121, 2; 397-400
0587-4246
1898-794X
Pojawia się w:
Acta Physica Polonica A
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Elemental Mapping of Prostate Tissue by Micro-SRIXE
Autorzy:
Banaś, A.
Banaś, K.
Falkenberg, G.
Dyduch, G.
Kwiatek, W. M.
Powiązania:
https://bibliotekanauki.pl/articles/2044669.pdf
Data publikacji:
2006-03
Wydawca:
Polska Akademia Nauk. Instytut Fizyki PAN
Tematy:
87.64.-t
Opis:
Synchrotron radiation-induced X-ray emission is now a proven analytical method for the determination of trace elements. Better insight of the role of trace elements in tissue samples can be gained by acquiring complete distribution maps of these elements. Our approach is based on the acquisition of digital maps. In this contribution we present two different methods for obtaining normalization matrix: first - based on argon intensity in the single spectrum and second - based on changes of beam current intensity. The region for the scan was carefully chosen according to the histological view of the sample. A total area was scanned with a step size of 15 μm in each direction and measuring time of 30 s per pixel. The thickness of the samples was 15 μm. A polycapillary was used for beam focusing. At the energy of 18 keV the beam size on the sample was approximately 15 μm which is of the order of a cell diameter.
Źródło:
Acta Physica Polonica A; 2006, 109, 3; 323-328
0587-4246
1898-794X
Pojawia się w:
Acta Physica Polonica A
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
$[FeCl_{4}]¯$ Ionic Liquid Based Ferrofluids
Autorzy:
Parnica, J.
Kesa, P.
Kovac, J.
Timko, M.
Antalik, M.
Powiązania:
https://bibliotekanauki.pl/articles/1368745.pdf
Data publikacji:
2014-07
Wydawca:
Polska Akademia Nauk. Instytut Fizyki PAN
Tematy:
87.64.kp
75.50.Bb
47.65.Cb
75.50.Mm
Opis:
Magnetic ionic liquids derived from hydrochloride methyl ester alanine amino acids (AA) and $FeCl_{3}$ were synthesized in ethanol and their magnetic properties were investigated. Iron (III) chloride forms ionic liquid in ethanol at saturated concentrations by the transfer of Cl¯ anion from one molecule of $FeCl_{3}$ to the other molecule with the creation of tetrachloro ferrate anion $[FeCl_{4}]$¯ as well as dimer $[Fe_{2}Cl_{7}]$¯. Raman scattering indicates that after addition of hydrochloride methyl ester alanine to ethanol solutions of $FeCl_{3}$ increase the intensities of signal at the frequencies at 318 $cm^{-1}$ and 380 $cm^{-1}$ as markers for the presence of $[FeCl_{4}]$¯ and $[Fe_{2}Cl_{7}]$¯ complexes. The magnetization at room temperature showed in both samples a linear dependence on the applied magnetic field. On the other hand, in the Raman scattering experiments the magnetization is smaller for $FeCl_{3}$ sample in the presence of methyl ester alanine.
Źródło:
Acta Physica Polonica A; 2014, 126, 1; 276-277
0587-4246
1898-794X
Pojawia się w:
Acta Physica Polonica A
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
X-Ray Fluorescence Techniques in Medical Applications: Reference Values of Elements in Human Serum, Urine and Hair
Autorzy:
Majewska, U.
Banaś, D.
Braziewicz, J.
Kubala-Kukuś, A.
Pajek, M.
Sychowska, I.
Wudarczyk-Moćko, J.
Antczak, G.
Borkowska, B.
Góźdź, S.
Powiązania:
https://bibliotekanauki.pl/articles/1197643.pdf
Data publikacji:
2014-04
Wydawca:
Polska Akademia Nauk. Instytut Fizyki PAN
Tematy:
32.30.Rj
87.64.kd
87.64.kv
Opis:
The aim of undertaken long-term studies of the elemental composition of human serum, urine, and hair is to define reference values of elements concentration. For this purpose the total reflection X-ray fluorescence method was applied to determination of several elements concentration in human serum, urine and hair (S, K, Ca, Fe, Cu, Zn, Br, P, Cr, and Rb in serum samples; Fe, Cu, Zn, Rb, Cr, Mn, and Sr in urine samples; S, K, Ca, Fe, Cu, Br, Zn, Cl, Ti, Cr, Mn, Ni, and Se in hair samples) in the range of concentration from ppb to several hundred ppm. The method of selection of the control group, the experimental setup and its calibration procedure are described. We also present sample preparation methods and procedure of measurements.
Źródło:
Acta Physica Polonica A; 2014, 125, 4; 864-868
0587-4246
1898-794X
Pojawia się w:
Acta Physica Polonica A
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Raman spectroscopy of shear modes in a few-layer MoS₂
Autorzy:
Gołasa, K.
Grzeszczyk, M.
Zinkiewicz, M.
Nogajewski, K.
Potemski, M.
Wysmołek, A.
Babiński, A.
Powiązania:
https://bibliotekanauki.pl/articles/1160654.pdf
Data publikacji:
2016-01
Wydawca:
Polska Akademia Nauk. Instytut Fizyki PAN
Tematy:
78.20.-e
87.64.Je
63.20.Kr
Opis:
We study low frequency vibrational modes in atomically thin molybdenum disulfide (MoS₂) by means of the Raman scattering spectroscopy. A shear mode related to rigid interlayer vibrations is identified. Its energy evolution with the increasing number of layers is well described using a linear chain model with only nearest neighbor interactions. The resulting force constant (Kₓ = 2.7 × 10¹⁹ N/m³) corresponds well to the previously published data.
Źródło:
Acta Physica Polonica A; 2016, 129, 1a; A-132-A-134
0587-4246
1898-794X
Pojawia się w:
Acta Physica Polonica A
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Preliminary Study on Chemical Speciation of Sulphur in Cancerous Tissues
Autorzy:
Kwiatek, W. M.
Banaś, A.
Banaś, K.
Kisiel, A.
Cinque, G.
Falkenberg, G.
Powiązania:
https://bibliotekanauki.pl/articles/2044744.pdf
Data publikacji:
2006-03
Wydawca:
Polska Akademia Nauk. Instytut Fizyki PAN
Tematy:
87.64.Gb
Opis:
Sulphur is vital for almost all living organisms by participating in a wide variety of metabolic processes. Nevertheless its biochemistry is only partially understood, due to it a few tools such as X-ray absorption near edge structure may be used to determine its chemical speciation in biological system. In this paper we focused on analysis of composition and elemental distribution in tissue structures of biological samples. We examined cancerous and non-cancerous prostate tissues. Experiments were carried out on the L-beam line at the HASYLAB, DESY (Hamburg, Germany) and DAΦNE-Light Facility at the Laboratori Nationali di Frascati (Frascati, Italy). Synchrotron radiation induced X-ray emission measurements reveal the significant differences in concentration of sulphur between cancerous and non-cancerous tissues. X-ray absorption near edge structure spectra show two main types of sulphur that are represented by X-ray peaks at 2476 eV and 2484 eV. This suggests that different sulphur components are present in the measured samples.
Źródło:
Acta Physica Polonica A; 2006, 109, 3; 383-387
0587-4246
1898-794X
Pojawia się w:
Acta Physica Polonica A
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Differentiation of Native and Reconstructed Ferritin using the MRI Gradient Echo Pulse Sequence
Autorzy:
Balejcikova, L.
Strbak, O.
Baciak, L.
Kovac, J.
Masarova, M.
Krafcik, A.
Kopcansky, P.
Dobrota, D.
Frollo, I.
Powiązania:
https://bibliotekanauki.pl/articles/1032829.pdf
Data publikacji:
2017-04
Wydawca:
Polska Akademia Nauk. Instytut Fizyki PAN
Tematy:
75.60.Ej
83.85.Fg
87.61.-c
87.64.Cc
87.85.jf
Opis:
Ferritin is a biological iron storage biomacromolecule, consisting of a spherical protein shell (apoferritin) and mineral iron core. It plays a crucial role in the pathological processes of disrupted iron homeostasis followed by iron accumulation, linked with various disorders (e.g. neuroinflammation, neurodegeneration, cirrhosis, cancer, etc.) In vitro reconstructed ferritin, with the assistance of a non-invasive magnetic resonance imaging technique, has the potential to become a suitable biomarker of these pathological processes. Through gradient echo pulse sequencing, we were able to clearly distinguish between native (physiological) and reconstructed/iron-overloaded (pathological) ferritin, which can serve as a starting point for the development of a method for their differentiation. Such method is necessary for the early diagnosis of iron-based diseases.
Źródło:
Acta Physica Polonica A; 2017, 131, 4; 1093-1095
0587-4246
1898-794X
Pojawia się w:
Acta Physica Polonica A
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Fourier Transform Near Infrared Raman Spectroscopy in Studies on Connective Tissue
Autorzy:
Połomska, M.
Kubisz, L.
Kalawski, R.
Oszkinis, G.
Filipiak, R.
Mazurek, A.
Powiązania:
https://bibliotekanauki.pl/articles/1537548.pdf
Data publikacji:
2010-07
Wydawca:
Polska Akademia Nauk. Instytut Fizyki PAN
Tematy:
78.30.-j
78.30.Jw
61.80.Ed
61.82.Pv
81.05.-t
87.-g
87.93.-j
87.64.K-
Opis:
Studies of tissue such as bones and veins often are carried out by means of microscopic and ultrasonographic methods. Therefore studies of changes caused by pathological or physical factors often are limited to morphological level. Application of Raman spectroscopy permits to associate morphological changes and molecular changes. The paper presents preliminary studies of temperature and ionizing radiation effects on Raman spectra of bone and collagen. Furthermore, effects of pathological factors on Raman spectra of human veins had been studied. It was noticed that heating of bone collagen up to 110°C gives the reversible changes in spectra. In the case of γ-irradiation, any effects were observed up to the dose of 1 MGy on mineral component of bone, whereas the influence on collagen was observed for doses higher than 100 kGy. The Raman spectra of varicose vs. normal veins permit to conclude some important changes observed for veins from chronic venous insufficiency patients.
Źródło:
Acta Physica Polonica A; 2010, 118, 1; 136-140
0587-4246
1898-794X
Pojawia się w:
Acta Physica Polonica A
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Magnetocaloric Properties of Fe₇₅Mo₈Cu₁B₁₆ and Fe₈₁Mo₈Cu₁B₁₀ Metallic Glasses
Autorzy:
Hasiak, M.
Miglierini, M.
Kaleta, J.
Bujdoš, M.
Powiązania:
https://bibliotekanauki.pl/articles/1387599.pdf
Data publikacji:
2015-02
Wydawca:
Polska Akademia Nauk. Instytut Fizyki PAN
Tematy:
75.30.Sg
71.23.Cq
76.80.+y
87.64.Pj
Opis:
Microstructure and thermomagnetic properties for the Fe₇₅Mo₈Cu₁B₁₆ and Fe₈₁Mo₈Cu₁B₁₀ metallic glasses in the as-quenched state and after heat treatment at 643 K and 723 K are studied. The inverse change of the Curie point was observed for Fe₇₅Mo₈Cu₁B₁₆ metallic glass after annealing below the onset of crystallization. It is attributed to structural relaxation of the amorphous phase. The maximum of magnetic entropy change calculated for magnetic field of 1.0 T occurs for the Fe₇₅Mo₈Cu₁B₁₆ alloy annealed at 643 K at temperature close to the Curie point and equals 0.74 J kg¯¹ K¯¹.
Źródło:
Acta Physica Polonica A; 2015, 127, 2; 608-610
0587-4246
1898-794X
Pojawia się w:
Acta Physica Polonica A
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Study of Adhesion Interaction Using Atomic Force Microscopy
Autorzy:
Gryboś, J.
Pyka-Fościak, G.
Lebed, K.
Lekka, M.
Stachura, Z.
Styczeń, J.
Powiązania:
https://bibliotekanauki.pl/articles/2038080.pdf
Data publikacji:
2004-05
Wydawca:
Polska Akademia Nauk. Instytut Fizyki PAN
Tematy:
87.64.Dz
68.35.Gy
Opis:
An atomic force microscope is a useful tool to study the interaction forces at molecular level. In particular the atomic force microscope can measure an unbinding force needed to separate the two single molecule complexes. Recent studies have shown that such unbinding force depends linearly on the logarithm of the applied loading rate, defined as a product of scanning velocity and the spring constant characterizing the investigated system (cantilever vs. surface). This dependence can be used to study the energy landscape shape of a molecular complex by the estimation of energy barrier locations and the related dissociation rates. In the present work the complex consisting of ethylene(di)aminetetraacetic acid and the bovine serum albumin was measured. The dependence between the unbinding force and the logarithm of the loading rate was linear. Using the Bell model describing the dissociation of the above molecules caused by the action of the external bond breaking force, two parameters were estimated: the dissociation rate and the position of the energy barrier needed to overcome during a transition from a bound to unbound state. The obtained results are similar to those obtained for a typical ligand-receptor interaction.
Źródło:
Acta Physica Polonica A; 2004, 105, 5; 501-510
0587-4246
1898-794X
Pojawia się w:
Acta Physica Polonica A
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Atomic Force Microscopy and Quartz Crystal Microbalance Study of the Lectin-Carbohydrate Interaction Kinetics
Autorzy:
Lebed, K.
Kulik, A. J.
Forró, L.
Lekka, M.
Powiązania:
https://bibliotekanauki.pl/articles/2047270.pdf
Data publikacji:
2007-02
Wydawca:
Polska Akademia Nauk. Instytut Fizyki PAN
Tematy:
87.64.Dz
84.37.+q
87.15.Kg
82.37.Np
81.65.Cf
52.77.Bn
Opis:
Two analytical methods, atomic force microscopy and quartz crystal microbalance, were applied to the study of the reaction kinetics occurring between concanavalin A and carboxypeptidase Y, presenting the specific lectin-carbohydrate recognition. The dissociation rate constants for concanavalin A-carboxypeptidase Y complex obtained using both atomic force microscopy and quartz crystal microbalance were of the same order of magnitude: k$\text{}_{diss}$=0.170± 0.060 s$\text{}^{-1}$ and k$\text{}_{diss}$=0.095±0.002 s$\text{}^{-1}$, respectively. In addition, each method alone aided in determining other parameters characterizing the studied interaction. Quartz crystal microbalance permitted us to estimate the association rate (k$\text{}_{ass}$=(5.6 ±0.1)×10$\text{}^{4}$ M$\text{}^{-1}$ s$\text{}^{-1}$) and the equilibrium (K$\text{}_{a}$=(0.59×0.01)×10$\text{}^{6}$ M$\text{}^{-1}$) constants for the binding process occurring between concanavalin A and mannose residues of carboxypeptidase Y under given experimental conditions. Atomic force microscopy in force spectroscopy mode enabled the determination of the energy barrier position of r=2.29±0.04 Å characterizing the dissociation of concanavalin A- carboxypeptidase Y molecular complex. The presented results show that both atomic force microscopy and quartz crystal microbalance can be used to determine quantitative parameters characterizing the specific molecular interaction. Both methods can be easily combined for complementary and/or alternative studies of a chosen molecular interaction. By preparing the samples in the same manner the direct comparison between the data obtained via atomic force microscopy and quartz crystal microbalance can be made.
Źródło:
Acta Physica Polonica A; 2007, 111, 2; 273-286
0587-4246
1898-794X
Pojawia się w:
Acta Physica Polonica A
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Optical Biopsy Method for Breast Cancer Diagnosis Based on Artificial Neural Network Classification οf Fluorescence Landscape Data
Autorzy:
Dramićanin, T.
Zeković, I.
Dimitrijević, B.
Ribar, S.
Dramićanin, M.
Powiązania:
https://bibliotekanauki.pl/articles/1795706.pdf
Data publikacji:
2009-10
Wydawca:
Polska Akademia Nauk. Instytut Fizyki PAN
Tematy:
87.64.kv
84.35.+i
87.19.xj
33.50.-j
Opis:
Supervised self-organizing map, a type of artificial neural network, is applied for classification of human breast tissue samples utilizing data obtained from fluorescence landscape measurements. Female breast tissue samples were taken soon after the surgical resection, identified and stored at -80°C until fluorescence measurements. From fluorescence landscapes obtained in UV-VIS region spectral features showing statistically significant differences between malignant and normal samples are identified and further quantified to serve as a training input to neural network. Additional set of samples was used as a test group input to trained network in order to evaluate performance of proposed optical biopsy method. Classification sensitivity of 83.9% and specificity of 88.9% are found.
Źródło:
Acta Physica Polonica A; 2009, 116, 4; 690-692
0587-4246
1898-794X
Pojawia się w:
Acta Physica Polonica A
Dostawca treści:
Biblioteka Nauki
Artykuł
Wyświetlanie 1-50 z 66

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