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    Wyświetlanie 1-6 z 6
    Tytuł:
    Identification of mare colostrum proteins
    Identyfikacja bialek siary klaczy – badania wstępne
    Autorzy:
    Medeńska, W.
    Dratwa-Chałupnik, A.
    Ożgo, M.
    Cichy, A.
    Pikuła, R.
    Bobik, J.
    Powiązania:
    https://bibliotekanauki.pl/articles/3131472.pdf
    Data publikacji:
    2020
    Wydawca:
    Zachodniopomorski Uniwersytet Technologiczny w Szczecinie. Wydawnictwo Uczelniane ZUT w Szczecinie
    Tematy:
    colostrum
    mare
    proteomics
    animal feeding
    functional protein
    foal growth
    development
    two-dimensional electrophoresis
    Źródło:
    Acta Scientiarum Polonorum. Zootechnica; 2020, 19, 4; 25-31
    1644-0714
    Pojawia się w:
    Acta Scientiarum Polonorum. Zootechnica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Elektroforeza swobodna z przepływem hydrodynamicznym : podstawy teoretyczne i rozwiązania techniczne
    Free flow electrophoresis : theory and technology
    Autorzy:
    Hoda, P.
    Polak, B.
    Dzido, T. H.
    Powiązania:
    https://bibliotekanauki.pl/articles/172756.pdf
    Data publikacji:
    2018
    Wydawca:
    Polskie Towarzystwo Chemiczne
    Tematy:
    elektroforeza swobodna
    przepływ hydrodynamiczny
    technika elektromigracyjna
    rozdzielanie pseudo-dwuwymiarowe
    rozdzielanie preparatywne
    mikro-elektroforeza swobodna
    free flow electrophoresis
    electromigration technique
    pseudo-two dimensional separation
    preparative separation
    micro free flow electrophoresis
    Opis:
    Free flow electrophoresis, or carrier-free electrophoresis, is a technique based upon the principle of mobility of chargeable species in the electric field. However, unlike more widespread gel electrophoresis, free flow electrophoresis does not utilise any kind of solid support medium. Instead, species to be separated move within the space filled with aqueous buffer, which is constantly pumped in the direction perpendicular to the direction of the applied electric field. Such set-up allows for continuous separation, as compounds of the sample being processed form separate bands and leave the separation system through several different outlets located along the edge of the chamber. Furthermore, the use of mild separation conditions increases the chance of biologically active compounds retaining their activity after the separation is finished. These qualities make free flow electrophoresis an excellent tool for protein research and cytology. In this review, the basic theoretical aspects of the technique are outlined, with a special emphasis placed on various modes in which free flow electrophoresis can be operated. Besides, a review of milestone papers related to free flow electrophoresis technology is presented. Apart from the devices following the original concept of Barrollier and Hannig, an insight into the construction of recirculating instrumentation, multicompartment electrolysers as well as radially-symmetric chambers is provided. A special focus is placed on patents and commercialized solutions. Finally, the challenges of scaling-down free flow electrophoresis to micro-dimensions are introduced.
    Źródło:
    Wiadomości Chemiczne; 2018, 72, 1-2; 39-60
    0043-5104
    2300-0295
    Pojawia się w:
    Wiadomości Chemiczne
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Proteome responses to herbicidal stress in maize
    Autorzy:
    Tyczewska, A.
    Bielinska, I.
    Gracz, J.
    Sikora, M.
    Twardowski, T.
    Powiązania:
    https://bibliotekanauki.pl/articles/951291.pdf
    Data publikacji:
    2015
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    abiotic stress
    plant response
    maize
    proteomic analysis
    gene expression
    two dimensional gel electrophoresis
    Źródło:
    BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2015, 96, 1
    0860-7796
    Pojawia się w:
    BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Primary results of 2-dimensional electrophoresis for protein studies of Gentiana kurroo Royle somatic embryos derived from long-term embryogenic cell suspensions
    Autorzy:
    Niedziela, A.
    Rybczynski, J.J.
    Powiązania:
    https://bibliotekanauki.pl/articles/56507.pdf
    Data publikacji:
    2014
    Wydawca:
    Polskie Towarzystwo Botaniczne
    Tematy:
    two dimensional gel electrophoresis
    protein
    Gentiana kurroo
    somatic embryo
    embryogenic cell
    cell suspension
    Opis:
    Two-dimensional gel electrophoresis was used to compare protein expression profiles between various stages of Gentiana kurroo Royle somatic embryos. Seven distinct stages (I–VII) were pointed out and measured from long-term embryogenic cell suspension. Isoelectric focusing was done in the pH intervals 3–10, and the second dimension was carried out with 13% SDS-polyacrylamide gel electrophoresis. Dependent on the stage from about 400 (stage IV) to more than 700 (stage II) protein spots were totally detected. The molecular weight of abundant proteins range from 12 to 70 kDa, however, majority of proteins were located between 20–49 kDa spots on the gels. The highest difference in the number of spots appeared in the case of globular embryo (stage I) and elongated cotyledonary stage (stage VII) with differences being about 130 spots. The relevance of embryogenic cell suspension choice for proteomic analysis as well as expediency of the increasing number of particular embryo stages is discussed.
    Źródło:
    Acta Societatis Botanicorum Poloniae; 2014, 83, 3
    0001-6977
    2083-9480
    Pojawia się w:
    Acta Societatis Botanicorum Poloniae
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Dwuwymiarowa separacja technikami chromatografii i elektroforezy ze szczególnym uwzględnieniem połączenia ich w proces jednoetapowy
    Two-dimensional separation with chromatography and electrophoresis techniques with special focus on their combination into single process
    Autorzy:
    Łopaciuk, E.
    Dzido, T. H.
    Powiązania:
    https://bibliotekanauki.pl/articles/171869.pdf
    Data publikacji:
    2013
    Wydawca:
    Polskie Towarzystwo Chemiczne
    Tematy:
    separacja dwuwymiarowa
    elektroforeza
    elektrochromatografia
    chromatografia cieczowa
    rozdzielanie dwuwymiarowe ciągłe
    two dimensional separation
    electrophoresis
    electrochromatography
    liquid chromatography
    continuous two-dimensional separation
    Opis:
    Liquid chromatography and electrophoresis techniques are very often applied in contemporary laboratory practice. These techniques usually show different separation selectivity. It is due to various separation mechanisms involved in these two modes. In the former, partition of solutes between stationary and mobile phases influences on separation selectivity and retention contrary to the latter in which electrophoretic effect is involved in separation mechanism. The features mentioned are very useful for combination of these two techniques into two-dimensional separation of complicated samples of biomedical and environmental origin. Development of such approach is a very promising for contemporary separation sciences. The paper presents an overview of two-dimensional separation techniques, in which both liquid chromatography and electrophoresis have been involved especially in continuous mode.
    Źródło:
    Wiadomości Chemiczne; 2013, 67, 9-10; 899-927
    0043-5104
    2300-0295
    Pojawia się w:
    Wiadomości Chemiczne
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Comparative proteomic analysis of Bombyx mori hemolymph and fat body after calorie restriction
    Autorzy:
    Chen, Huiqing
    Li, Yijia
    Chen, Keping
    Yao, Qin
    Li, Guohui
    Wang, Lin
    Powiązania:
    https://bibliotekanauki.pl/articles/1040312.pdf
    Data publikacji:
    2010
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    calorie restriction
    Bombyx mori
    two-dimensional gel electrophoresis
    proteomic analysis
    MALDI-TOF/TOF MS
    Opis:
    Calorie restriction (CR) is known to extend life span from yeast to mammals. To gain an insight into the effects of CR on growth and development of the silkworm Bombyx mori at protein level, we employed comparative proteomic approach to investigate proteomic differences of hemolymph and fat body of the silkworm larvae subjected to CR. Thirty-nine differentially expressed proteins were identified by MALDI TOF/TOF MS. Among them, 19 were from the hemolymph and 20 from the fat body. The hemolymph of the CR group contained two down-regulated and 17 up-regulated proteins, whereas the fat body contained 15 down-regulated and five up-regulated ones. These proteins belonged to those functioning in immune system, in signal transduction and apoptosis, in regulation of growth and development, and in energy metabolism. Our results suggest that CR can alter the expression of proteins related to the above four aspects, implying that these proteins may regulate life span of the silkworm through CR.
    Źródło:
    Acta Biochimica Polonica; 2010, 57, 4; 505-511
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-6 z 6

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