Dwuwymiarowa separacja technikami chromatografii i elektroforezy ze szczególnym uwzględnieniem połączenia ich w proces jednoetapowy

Liquid chromatography and electrophoresis techniques are very often applied in contemporary laboratory practice. These techniques usually show different separation selectivity. It is due to various separation mechanisms involved in these two modes. In the former, partition of solutes between stationary and mobile phases influences on separation selectivity and retention contrary to the latter in which electrophoretic effect is involved in separation mechanism. The features mentioned are very useful for combination of these two techniques into two-dimensional separation of complicated samples of biomedical and environmental origin. Development of such approach is a very promising for contemporary separation sciences. The paper presents an overview of two-dimensional separation techniques, in which both liquid chromatography and electrophoresis have been involved especially in continuous mode.